Invertebrate dispersal by waterbird species in neotropical wetlands / A dispersão de invertebrados por aves aquáticas na região neotropical

Abstract Endozoochory by waterbirds is particularly relevant to the dispersal of non-flying aquatic invertebrates. This ecological function exercised by birds has been demonstrated in different biogeographical regions, but there are no studies for the neotropical region. In this work, we identified propagules of invertebrates in faeces of 14 syntopic South American waterbird species representing six families, and hatched additional invertebrates from cultured faeces. We tested whether propagule abundance, species richness and composition varied among bird species, and between the cold and warm seasons. We found 164 invertebrate propagules in faecal samples from seven different waterbirds species, including eggs of the Temnocephalida and Notonectidae, statoblasts of bryozoans (Plumatella sp.) and ephippia of Cladocera. Ciliates (including Paramecium sp. and Litostomatea), nematodes and rotifers (Adineta sp. and Nottomatidae) hatched from cultured samples. Potential for endozoochory was confirmed for 12 of 14 waterbird species. Our statistical models suggest that richness and abundance of propagules are associated with bird species and not affected by seasonality. Dispersal by endozoochory is potentially important to a broad variety of invertebrates, being promoted by waterbirds with different ecological and morphological traits, which are likely to drive the dispersal of invertebrates in neotropical wetlands.

Resumo A endozoocoria promovida por aves aquáticas é particularmente relevante para a dispersão de invertebrados aquáticos não-voadores. Essa função ecológica exercida pelas aves tem sido demonstrada para diferentes regiões biogeográficas, porém, não existem estudos para a região neotropical. Neste trabalho nós identificamos propágulos de invertebrados encontrados em fezes de 14 espécies sintópicas de aves aquáticas da América do Sul, representando seis famílias de aves, e também invertebrados emergidos de amostras fecais cultivadas em laboratório. Testamos se a abundância, riqueza de espécies e composição de propágulos de invertebrados variavam entre as espécies de aves e entre estações. Nós encontramos 164 propágulos de invertebrados em amostras fecais de sete espécies de aves, incluindo ovos de Temnocephalida e Notonectidae, estatoblastos de briozoários (Plumatella sp.) e efípios de Cladocera. Ciliados (incluindo Paramecium sp. e Litostomatea), nematóides e rotíferos (Adineta sp. e Nottomatidae) eclodiram de amostras cultivadas. O potencial para endozoocoria foi confirmado para 12 das 14 espécies de aves aquáticas investigadas. Nossos modelos estatísticos sugerem que a riqueza e abundância de propágulos estão associadas às espécies de aves e não são afetadas pela sazonalidade. A dispersão por endozoocoria é importante para uma ampla variedade de invertebrados, sendo promovida por aves aquáticas com diferentes características ecológicas e morfológicas as quais provavelmente regulam a dispersão de invertebrados entre áreas úmidas neotropicais.

Benthic community ecology for Algerian river Seybouse / Ecologia da comunidade bêntica para o rio Seybouse da Argélia

Abstract The Seybouse is the second largest river basin in Algeria, hosting an important biodiversity and providing various ecosystem services. This watershed is highly influenced by agricultural and industrial activities, which threaten its biodiversity and ecosystem integrity. The use of benthic macroinvertebrates as biological indicators has a long tradition in developed countries and integrated into all assessments of the ecological quality of river systems. However, the macroinvertebrates of many North African regions are still not well studied, including those of the Seybouse river. The aim of this study is to assess the inventory and ecological role of benthic macroinvertebrates in inland waters of the Seybouse River and determine the impact of pollution on their spatial distributions. We sampled the benthic macrofauna of Wadi Seybouse and its affluents using regular surveys in three sites, of which one was in the upper Seybouse Bouhamdane in Medjez Amar and two in the middle Seybouse. Between December 2019 and May 2020, 10 physico-chemical parameters (pH, EC, OD, water speed, NO3, Salinity, NO2, MES, turbidity, depth) were measured in order to establish a health state diagnosis of these aquatic ecosystems. The complementary biological approach by the analysis of populations of macroinvertebrates identified 7482 individuals and 40 taxa divided into five classes: Crustaceans which were the most dominant, insects with the main orders (Ephemeroptera, Diptera, Trichoptera, Heteroptera and Odonata), Molluscs, Nematodes and Annelids. The physico-chemical analyzes and the application of the organic pollution indices indicated a strong to excessive pollution for all sites, especially in Seybouse upstream

Resumo O Seybouse é um rio no nordeste da Argélia, é o segundo maior rio, tem uma área de captação de cerca de 6.500 km2 que acolhe cerca de 1,5 milhões de habitantes. Importantes atividades agrícolas e industriais são desenvolvidas nesta bacia hidrográfica. O uso de macroinvertebrados bentônicos como indicadores biológicos tem uma longa tradição em países desenvolvidos e está integrado em todas as avaliações da qualidade ecológica dos sistemas fluviais. O objetivo deste estudo foi revisar o inventário e o papel ecológico dos macroinvertebrados bentônicos nas águas interiores do rio Seybouse e determinar o impacto da poluição em suas distribuições. Resultados semelhantes foram relatados para outros países e rios argelinos. Este estudo enfoca a macrofauna bentônica de Wadi Seybouse e seus afluentes. Foram prospectados 03 locais, um no alto Seybousse Bouhamdane em Medjez Amar e dois no meio Seybousse Salah Salah Salah e Oued Zimba. Entre dezembro de 2019 e maio de 2020, dez parâmetros físico-químicos (pH, CE, DO, velocidade da água, NO3, Salinidade, NO2, MES, turbidez, deth) foram medidos para estabelecer um diagnóstico do estado de saúde desses ecossistemas aquáticos. A abordagem biológica complementar pela análise de populações de macroinvertebrados identificou 7.482 indivíduos e 40 táxons divididos em cinco classes: Crustáceos que são os mais dominantes, Insetos com as ordens principais (Ephemeroptera, Diptera, Trichoptera, Heteroptera e Odonata), Moluscos, Nematóides e Anelídeos. As análises físico-químicas e a aplicação dos índices de poluição orgânica, indicaram uma poluição forte a excessiva para todos os locais especialmente Salah Salah Salah.

Bayesian analyses indicate bivalves did not drive the downfall of brachiopods following the Permian-Triassic mass extinction.

Certain times of major biotic replacement have often been interpreted as broadly competitive, mediated by innovation in the succeeding clades. A classic example was the switch from brachiopods to bivalves as major seabed organisms following the Permian-Triassic mass extinction (PTME), ~252 million years ago. This was attributed to competitive exclusion of brachiopods by the better adapted bivalves or simply to the fact that brachiopods had been hit especially hard by the PTME. The brachiopod-bivalve switch is emblematic of the global turnover of marine faunas from Palaeozoic-type to Modern-type triggered by the PTME. Here, using Bayesian analyses, we find that unexpectedly the two clades displayed similar large-scale trends of diversification before the Jurassic. Insight from a multivariate birth-death model shows that the extinction of major brachiopod clades during the PTME set the stage for the brachiopod-bivalve switch, with differential responses to high ocean temperatures post-extinction further facilitating their displacement by bivalves. Our study strengthens evidence that brachiopods and bivalves were not competitors over macroevolutionary time scales, with extinction events and environmental stresses shaping their divergent fates.

Organ-oriented proteogenomics functional atlas of three aquatic invertebrate sentinel species.

Proteogenomic methodologies have enabled the identification of protein sequences in wild species without annotated genomes, shedding light on molecular mechanisms affected by pollution. However, proteomic resources for sentinel species are limited, and organ-level investigations are necessary to expand our understanding of their molecular biology. This study presents proteomic resources obtained from proteogenomic analyses of key organs (hepatopancreas, gills, hemolymph) from three established aquatic sentinel invertebrate species of interest in ecotoxicological/ecological research and environmental monitoring: Gammarus fossarum, Dreissena polymorpha, and Palaemon serratus. Proteogenomic analyses identified thousands of proteins for each species, with over 90% of them being annotated to putative function. Functional analysis validated the relevance of the proteomic atlases by revealing similarities in functional annotation of catalogues of proteins across analogous organs in the three species, while deep contrasts between functional profiles are delimited across different organs in the same organism. These organ-level proteomic atlases are crucial for future research on these sentinel animals, aiding in the evaluation of aquatic environmental risks and providing a valuable resource for ecotoxicological studies.

Metabolomic responses in freshwater benthic invertebrate, Chironomus tepperi, exposed to polyethylene microplastics: A two-generational investigation.

The accumulation of microplastics (MPs) in sediments could pose risks to benthic organisms and their progeny. Here, we examined effects on traditional apical endpoints along with changes to whole body metabolite profiles induced by irregular shaped polyethylene MPs (1-45 µm) at environmentally relevant concentrations (125, 250, 500 and 1000 MPs/kg sediment) in Chironomus tepperi using a two-generation exposure regime. Survival and emergence of C. tepperi were negatively affected in the parental generation at the two highest concentrations, whereas endpoints associated with growth were only impacted at 1000 MPs/kg sediment. Metabolites associated with several amino acid and energy metabolism pathways were present at lower abundances at the highest exposure concentration suggesting an overall impact on bioenergetics which relates to the inhibition of food acquisition or nutrient assimilation caused by ingestion of MPs, rather than a traditional receptor-mediated toxicity response. In contrast, no significant effects on apical endpoints were observed in the continuous exposure of first filial generation, and lactic acid was the only metabolite that differed significantly between groups. Larvae in unexposed conditions showed no differences in survival or metabolite profiles suggesting that effects in the parental generation do not carry over to the next filial generation. The findings provide evidence on the underlying impacts of MP ingestion and potential adaption to MP exposure of C. tepperi.

The synaptic basis for sexual dimorphism in the invertebrate nervous system.

Many animal behaviors are manifested differently in the two sexes of a given species, but how such sexual dimorphism is imprinted in the nervous system is not always clear. One mechanism involved is synaptic dimorphism, by which the same neurons exist in the two sexes, but form synapses that differ in features such as anatomy, molecular content or fate. While some evidence for synaptic dimorphism exists in humans and mammals, identifying these mechanisms in invertebrates has proven simpler, due to their smaller nervous systems and absence of external regulation by sex hormones. This review aims to present the current status of the field in invertebrates, the available toolkit for the study of synaptic dimorphism, and the standing questions that still remain incompletely answered.

The mercury flow through a terrestrial songbird food chain in subtropical pine forest: Elucidated by Bayesian isotope mixing model and stable mercury isotopes.

In order to comprehend the transfer of inorganic mercury (IHg) and methylmercury (MeHg) within food chains in terrestrial pine forests, we collected samples of Great Tit nestlings, common invertebrates, plants, and soil in a subtropical pine forest and used Bayesian isotope mixing model analysis, Hg daily intake, and stable Hg isotopes to elucidate the flow of MeHg and IHg in these food chains. Results indicate that caterpillars and cockroaches are the predominant prey items for nestlings, accounting for a combined contribution of 81.5%. Furthermore, caterpillars, cockroaches, and spiders were found to contribute the most (∼80%) of both IHg and MeHg that dietary accumulated in nestlings. The provisoned invertebrates tend to supply more IHg and diluting the proportion of MeHg as total Hg (MeHg%). Notably, nestling feathers displayed the highest Δ199Hg values but a relatively lower MeHg%, suggesting an imbalanced incorporation of Hg from maternal transfer and dietary accumulation during the nestling stage. This study highlights the efficacy of nestlings as indicators for identifying Hg sources and transfers in avian species and food chains. However, caution must be exercised when using Hg isotope compositions in growing feathers, and the contribution of maternally transferred Hg should not be ignored.

The recovery of European freshwater biodiversity has come to a halt.

Owing to a long history of anthropogenic pressures, freshwater ecosystems are among the most vulnerable to biodiversity loss1. Mitigation measures, including wastewater treatment and hydromorphological restoration, have aimed to improve environmental quality and foster the recovery of freshwater biodiversity2. Here, using 1,816 time series of freshwater invertebrate communities collected across 22 European countries between 1968 and 2020, we quantified temporal trends in taxonomic and functional diversity and their responses to environmental pressures and gradients. We observed overall increases in taxon richness (0.73% per year), functional richness (2.4% per year) and abundance (1.17% per year). However, these increases primarily occurred before the 2010s, and have since plateaued. Freshwater communities downstream of dams, urban areas and cropland were less likely to experience recovery. Communities at sites with faster rates of warming had fewer gains in taxon richness, functional richness and abundance. Although biodiversity gains in the 1990s and 2000s probably reflect the effectiveness of water-quality improvements and restoration projects, the decelerating trajectory in the 2010s suggests that the current measures offer diminishing returns. Given new and persistent pressures on freshwater ecosystems, including emerging pollutants, climate change and the spread of invasive species, we call for additional mitigation to revive the recovery of freshwater biodiversity.

Fascinating Furanosteroids and Their Pharmacological Profile.

This review article delves into the realm of furanosteroids and related isoprenoid lipids derived from diverse terrestrial and marine sources, exploring their wide array of biological activities and potential pharmacological applications. Fungi, fungal endophytes, plants, and various marine organisms, including sponges, corals, molluscs, and other invertebrates, have proven to be abundant reservoirs of these compounds. The biological activities exhibited by furanosteroids and related lipids encompass anticancer, cytotoxic effects against various cancer cell lines, antiviral, and antifungal effects. Notably, the discovery of exceptional compounds such as nakiterpiosin, malabaricol, dysideasterols, and cortistatins has revealed their potent anti-tuberculosis, antibacterial, and anti-hepatitis C attributes. These compounds also exhibit activity in inhibiting protein kinase C, phospholipase A2, and eliciting cytotoxicity against cancer cells. This comprehensive study emphasizes the significance of furanosteroids and related lipids as valuable natural products with promising therapeutic potential. The remarkable biodiversity found in both terrestrial and marine ecosystems offers an extensive resource for unearthing novel biologically active compounds, paving the way for future drug development and advancements in biomedical research. This review presents a compilation of data obtained from various studies conducted by different authors who employed the PASS software 9.1 to evaluate the biological activity of natural furanosteroids and compounds closely related to them. The utilization of the PASS software in this context offers valuable advantages, such as screening large chemical libraries, identifying compounds for subsequent experimental investigations, and gaining insights into potential biological activities based on their structural features. Nevertheless, it is crucial to emphasize that experimental validation remains indispensable for confirming the predicted activities.

Ecological structure of aquatic macroinvertebrate communities in the Hauts Plateaux of Northeast Algeria.

Aquatic macroinvertebrates are integral parts of functioning wetlands, inhabiting a diversity of aquatic ecosystems where communities are spatially structured. Macroinvertebrates of some Mediterranean regions such as North Africa are still not well studied. Here, we study the community structure of benthic macroinvertebrates in four different types of lotic and lentic wetlands in the semiarid Haut Plateaux of Northeast Algeria. Macroinvertebrates and 06 physicochemical parameters were sampled in 12 sites belonging to four types of habitats (lotic river, lentic river, dams, and sebkha [saline lentic water body]) during January-December 2021. Eventually, the character lentic-lotic was the most important variable affecting the affecting invertebrate communities in the Mediterranean region. The results revealed that sebkha and dams had a lower Shannon index than lentic and lotic rivers. Non-metric multidimensional scaling analysis showed a strong overlap between the community composition in lotic and lentic sites . Howeverin the three types of aquatic groups lentic river, dam and lotic habitats showed a strong overlap between the community whereas sebkha was markedly separated. Redundancy analysis showed that water velocity and pH, were the main drivers of community structure of macroinvertebrates, revealed a strong effect with pH (F1,8=4.15, P = 0.001) and water velocity (F1,8= 3.22, P = 0.002) , separating lotic communities from those that inhabited dams, lentic rivers, and sebkhas. As conclusion, this study contributes to the better understanding of the community structure of macroinvertebrates in semiarid North Africa where wetlands have been experiencing high anthropogenic disturbance.

Validation of biomarkers for neonicotinoid exposure in Folsomia candida under mutual exposure to diethyl maleate.

Neonicotinoid insecticides are harmful to non-target soil invertebrates, which are crucial for sustainable agriculture. Gene expression biomarkers could provide economic and high-throughput metrics of neonicotinoid exposure and toxicity to non-target invertebrates. Thereby, biomarkers can help guide remediation efforts or policy enforcement. Gene expression of Glutathione S-Transferase 3 (GST3) has previously been proposed as a biomarker for the neonicotinoid imidacloprid in the soil ecotoxicological model species Folsomia candida (Collembola). However, it remains unclear how reliably gene expression of neonicotinoid biomarkers, such as GST3, can indicate the exposure to the broader neonicotinoid family under putative GST enzymatic inhibition. In this work, we exposed springtails to two neonicotinoids, thiacloprid and imidacloprid, alongside diethyl maleate (DEM), a known GST metabolic inhibitor that imposes oxidative stress. First, we determined the influence of DEM on neonicotinoid toxicity to springtail fecundity. Second, we surveyed the gene expression of four biomarkers, including GST3, under mutual exposure to neonicotinoids and DEM. We observed no effect of DEM on springtail fecundity. Moreover, the expression of GST3 was only influenced by DEM under mutual exposure with thiacloprid but not with imidacloprid. The results indicate that GST3 is not a robust indicator of neonicotinoid exposure and that probable GST enzymatic inhibition mediates the toxicity of imidacloprid and thiacloprid differentially. Future research should investigate biomarker reliability under shifting metabolic conditions such as provided by DEM exposure.

Transcriptomic sequencing data illuminate insecticide-induced physiological stress mechanisms in aquatic non-target invertebrates.

Pesticides are major agricultural stressors for freshwater species. Exposure to pesticides can disrupt the biotic integrity of freshwater ecosystems and impair associated ecosystem functions. Unfortunately, physiological mechanisms through which pesticides affect aquatic organisms are largely unknown. For example, the widely-used insecticide chlorantraniliprole is supposed to be highly selective for target pest species, i.e. Lepidoptera (butterflies), but its effect in aquatic non-target taxa is poorly studied. Using RNA-sequencing data, we quantified the insecticide effect on three aquatic invertebrate species: the caddisfly Lepidostoma basale, the mayfly Ephemera danica and the amphipod Gammarus pulex. Further, we tested how the insecticide-induced transcriptional response is modulated by biotic interaction between the two leaf-shredding species L. basale and G. pulex. While G. pulex was only weakly affected by chlorantraniliprole exposure, we detected strong transcriptional responses in L. basale and E. danica, implying that the stressor receptors are conserved between the target taxon Lepidoptera and other insect groups. We found in both insect species evidence for alterations of the developmental program. If transcriptional changes in the developmental program induce alterations in emergence phenology, pronounced effects on food web dynamics in a cross-ecosystem context are expected.

Optical Control of Invertebrate nAChR and Behaviors with Dithienylethene-Imidacloprid.

Photopharmacology involving azobenzene or dithienylethene has changed established methods of studying receptor functions, allowing for increasing the spatiotemporal resolution. There are no photopharmacological tools available for the invertebrate nicotinic acetylcholine receptor (nAChR). Here, we report a photochromic ligand, dithienylethene-imidacloprid (DitIMI), using a dithienylethene photoswitch embedded in the nAChR agonist imidacloprid. It was found that DitIMI displayed good photochromism and fluorescence switching behaviors upon irradiation with UV/vis light in aqueous solution. We demonstrated that open-DitIMI has low spontaneous in vitro and in vivo activity but can be photoisomerized to a highly active closed-form. Surprisingly, the photoswitchable DitIMI showed a large difference in insecticidal activity between the open and closed forms, in which the insecticidal activity against Aphis craccivora of the ring-closed isomers for DitIMI was 355 times that of the corresponding ring-open isomers. This photoisomerization can further be translated to photomodulation of neuron membrane potential and behavioral responses of living mosquito larvae and American cockroaches. The photomanipulation of nACh neurotransmission opens new avenues to understanding inhibitory circuits in intact animals.

Latest Triassic and Early Jurassic Spiriferinida (Brachiopoda) of Zealandia(New Zealand and New Caledonia).

The Late Triassic spiriferinides of Zealandia include the endemic or Austral genera Rastelligera, Psioidea and Psioidiella, and the cosmopolitan Zugmayerella. Mentzelia kawhiana is revised and placed in Callospiriferina. It appears in the late Rhaetian and is found in the Téremba Terrane in New Caledonia and throughout the Murihiku Terrane in New Zealand. The spiriferinides were severely affected at the Triassic-Jurassic boundary in Zealandia as elsewhere, but a moderately diverse fauna developed in the Early Jurassic. This has strong links to South America, and affinities with southern Europe and North Africa. In this study, a total of six species of Jurassic spiriferinides are recognised. Callospiriferina ongley is present in middle and late Hettangian and Sinemurian faunas. It is succeeded in the Pliensbachian and early Toarcian by Callospiriferina radiata. Two species of Spiriferina are recognised. S. sophiaealbae n. sp. first appears in the Middle Hettangian and is present in the Southland and Kawhia Synclines until the early Toarcian. S. arakiwa n. sp. is found mainly on the southwest limb of the Southland Syncline in the Pliensbachian and early Toarcian. The non-costate European genus Cisnerospira is represented in Zealandia by the small C. antipoda n. sp. This species ranges from Hettangian to early Toarcian. Two specimens of a spiriferinide with a costate sulcus are tentatively identified as Dispiriferina sp. cf. D. chilensis. The highest stratigraphic level at which Zealandian spiriferinides have been found is that of the Dactylioceras band at Kawhia, which is correlated with the Crassum Subzone of the Bifrons Zone (highest Early Toarcian) and includes three species of spiriferinide. This suggests that the Zealandian spiriferinides survived the Toarcian Event, only to meet their demise slightly later.

Assemblages of Trichoptera larvae on water moss in the middle reaches of the Yenisei River (Siberia, Russia).

This study provides first data on species composition of trichopterans inhabiting water moss, Fontinalis antipyretica Hedw., in the middle reaches of the Yenisei River. Analysis of Trichoptera larvae collected from samples of water moss in tail waters of the Krasnoyarsk Hydroelectric Plant in 2008-2021 revealed nine species of the order, which belonged to four families. Among them, six species belonging to the Limnephilidae family have not been mentioned in previous studies of the Yenisei invertebrates. Images of species are provided. One species, Apatania crymophyla McLachlan, was the most dense in all samples of water moss. The densities of A. crymophyla in samples of water moss in 2021 ranged within 1.2-7.0 thousand individuals / kg f.w. Other representatives of the order occurred in samples of water moss sporadically or consistently but in very low densities, which did not allow reliable quantification of their abundances. Consequently, numerous large clumps of water moss in the fast-flowing waters of the Yenisei favor an abundance of Trichoptera larvae, providing these insects refuge, food, and material for their cases. However, the reasons for the predominance of one species, A. crymophyla, are to be elucidated in further studies. The present study suggests that the contributions of Trichoptera and the total invertebrate community inhabiting water moss to biomass and energy budgets in the Yenisei have been usually underestimated before now. Hence, seasonal trends of species composition and abundance of invertebrates associated with water moss in the Yenisei River, including comparison with the entire zoobenthic community, are to be studied in the future.

Community recovery of benthic macroinvertebrates in a stream influenced by mining activity: Importance of microhabitat monitoring.

The decrease in freshwater biodiversity owing to anthropogenic disturbances such as mining activity is a global challenge; hence, there is an urgent need for systematic approaches to continuously monitor such disturbances and/or the recovery of biodiversity in freshwater habitats. The Hwangjicheon Stream is the source of South Korea's longest river and has been subjected to runoff from coal mining. We investigated changes in the diversity of the benthic macroinvertebrate community in various microhabitats, including riffle, run, and pool, to monitor the recovery of biodiversity in the stream following the improvement of a mining water treatment plant in 2019. The dataset comprised 111 samples obtained from four types of microhabitats (riffle, run, pool, and riparian) over a four-year period from 2018 to 2021. The mining-affected sites had lower macroinvertebrate community complexities according to a network analysis, and grouped into the same cluster based on self-organizing map (SOM) analysis. Moreover, 51 taxa selected as indicator species represented each cluster obtained through the SOM analysis. Among them, only Limnodrilus gotoi and Radix auricularia were included as indicator species at the mining-affected sites. However, after 2020, the benthic macroinvertebrate community complexity increased, and some of the microhabitats at the mining-affected sites were included in the same cluster as the reference sites in the SOM analysis, indicating that the recovery of benthic macroinvertebrate communities had initiated in certain microhabitats (e.g., riparian). Further analysis confirmed that the macroinvertebrate community clearly differed according to the survey year, even in different microhabitats at the same sites. This suggests that more acute microhabitat monitoring may be necessary to quickly confirm biodiversity restoration when assessing the degree of the recovery in river biodiversity from anthropogenic disturbances.

Stream Habitats and Aquatic Communities in an Agricultural Watershed: Changes Related to a Mandatory Riparian Buffer Law.

Riparian buffers along streams can intercept eroding soils, contaminants, and nutrients, improving stream habitats and increasing the health of aquatic communities. Instream and riparian habitats and fish and benthic invertebrate communities were surveyed in a Minnesota stream draining an agricultural watershed before and after implementation of a state-mandated buffer law passed in 2014 and aimed at protecting water quality. Intensive habitat assessments, electrofishing, and benthic invertebrate sampling were used at the same 13 sites in 2005 and 2018. Average buffer width nearly doubled between surveys, and instream abundance of fine sediments and embeddedness of coarse substrates by fine sediments both declined significantly within 1 to 3 years of buffer establishment. Stream sites also were significantly deeper with faster current velocities, and sites had increased riffle habitat and increased instream vegetative cover for fish after buffer mandates. However, fish and invertebrate biotic integrity scores, and other biotic community metrics, did not display significant improvements after buffer establishment. Stream habitats appear to improve quickly when intact and continuous riparian buffers insulate streams from surrounding agricultural activities, but improvements in biotic communities likely will require more time to adapt to changed habitat conditions.