Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 4.557
Filtrar
1.
Braz. j. oral sci ; 21: e224013, jan.-dez. 2022. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1354702

RESUMO

Aim: The aim of the study was to evaluate the cleaning of mandibular incisors with WaveOne Gold® (WO) under different preparation techniques. Methods: A total of 210 human mandibular incisors were selected and divided into seven groups (n = 30), prepared by WO single-files (Small 20/.07 ­ WOS; Primary 25/.07 ­ WOP; Medium 35/.06 ­ WOM; or Large 45/.05 - WOL) and sequential-file techniques (WOS to WOP; WOS to WOM; and WOS to WOL). Further subdivision was made according to irrigation protocol: control group (manual irrigation - CON), E1 Irrisonic® - EIR, and EDDY® - EDD. Debris removal and the smear layer were evaluated by scanning electron microscopy. Data were analyzed by using Spearman's correlation test. The significance level was set at 5%. Results: For debris and smear layer removal, WOS and WOP, EIR differed from CON and EDD (p <0.05). Conclusion: Regardless of the instrumentation used, the agitation of the irrigant solution provided better cleanability. These findings reinforce the need for agitation techniques as adjuvants in cleaning root canal systems in mandibular incisors


Assuntos
Irrigantes do Canal Radicular , Microscopia Eletrônica de Varredura , Preparo de Canal Radicular , Endodontia
2.
J Evid Based Dent Pract ; 22(2): 101728, 2022 06.
Artigo em Inglês | MEDLINE | ID: mdl-35718432

RESUMO

ARTICLE TITLE AND BIBLIOGRAPHIC INFORMATION: Báez, Viviana; Corcos, Lorena; Morgillo, Florencia; Imperatrice, Lorena; Gualtieri, Ariel Félix (2022). Meta-analysis of regenerative endodontics outcomes with antibiotics pastes and calcium hydroxide. The apex of the iceberg. J Oral Biol Craniofac Res 12(1): 90-98. SOURCE OF FUNDING: Non-funded study TYPE OF STUDY/DESIGN: Systematic review and meta-analysis.


Assuntos
Hidróxido de Cálcio , Endodontia Regenerativa , Antibacterianos , Dentina , Humanos , Irrigantes do Canal Radicular
3.
Niger J Clin Pract ; 25(6): 895-902, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35708431

RESUMO

Background: The whole root canal disinfection is crucial in terms of long-term endodontic success. In this context, applying medicaments following effective irrigation activation procedures becomes an important point. Aims: The purpose of this study was to evaluate and compare the efficacy of various irrigation activation techniques on non-infected dentinal tubule penetration of calcium hydroxide (CH), double (DAP), and triple (TAP) antibiotic pastes. Materials and Methods: A total of 180 extracted human permanent mandibular premolar single-rooted teeth were selected and randomly divided into a control group and four main groups according to the irrigation activation procedures (n = 36) as KTP laser irradiation, conventional needle, NaviTip FX, sonic (SI) and ultrasonic activation (UI) procedures. Each group was randomly subdivided into three subgroups according to the medicament type (n = 12); CH, DAP, and TAP. After the activation procedures, the root canals were filled with CH, TAP, and DAP mixed with 0.1% fluorescent rhodamine B isothiocyanate. Specimens were sectioned at 2, 5, and 8 mm from the apex, and all the sections were examined under a confocal laser scanning microscope to calculate the dentinal tubule penetration. Data were analyzed using a three-way analysis of variance and Tukey's post hoc tests (P = 0.05). Results: TAP provided a statistically significant greater penetration than the other groups (P < 0.05). UI provided a statistically significant higher dentinal tubules penetration area than other activation procedures (P < 0.05). Conclusion: Medicament penetration depends on the type of medicament, activation procedures, and root canal level. The TAP may be preferred following the ultrasonic activation in terms of long term root canal treatment success.


Assuntos
Dentina , Irrigantes do Canal Radicular , Antibacterianos/farmacologia , Hidróxido de Cálcio/farmacologia , Cavidade Pulpar , Humanos , Irrigantes do Canal Radicular/farmacologia , Preparo de Canal Radicular/métodos , Raiz Dentária
5.
Niger J Clin Pract ; 25(5): 670-676, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35593611

RESUMO

Background: Irrigant activation techniques, which are more effective in anatomically complex areas, can be used to maximize irrigant efficacy. Aim: This in vitro study aimed to evaluate the efficacy of different agitation techniques on the dislocation resistance of Biodentine to the root canal dentin. Materials and Methods: Seventy single-rooted teeth divided into seven experimental groups (n = 10); Group I-Irritrol/Photon-induced-photoacoustic-streaming (PIPS), Group II-Irritrol/EDDY®, Group III- Irritrol/Syringe-needle-irrigation (SNI), Group IV-Chlorhexidine-gluconate (CHX)/PIPS, Group V-CHX/EDDY®, Group VI-CHX/SNI, Group VII-Saline. The midroot dentin slice was obtained from each tooth, and Biodentine was condensed with hand pluggers into the root canal lumen. The push-out bond strength values were measured using a universal testing machine. Each sample was categorized into one of the three failure modes: adhesive/cohesive/mixed. Scanning-Electron-Microscopy (SEM) was used to conduct the analyses, and the composition of Biodentine was analyzed using Energy-Dispersive Spectroscopy. The One-way ANOVA, post-hoc Tukey's test, and the Chi-square test were used for statistical analysis. Results: The push-out bond strength values of Biodentine showed that Group VII-Saline had a statistically significant difference (P = 0.002), however, the differences between the other groups were not statistically significant (P = 0.922). The percentages of the failure modes of the samples showed that there was a higher rate of mixed failure except for Group VII-Saline. SEM examination showed that Group VII-Saline had no open dentinal tubules, whereas the other groups, particularly the Irritrol groups, had open dentinal tubule areas. Conclusions: Within the scope of the study, using Irritrol or CHX as the final irrigation in the root canal treatment did not result in differences in the dislocation resistance of Biodentine to root canal dentin when PIPS and EDDY® were used.


Assuntos
Clorexidina , Colagem Dentária , Compostos de Cálcio , Clorexidina/química , Clorexidina/farmacologia , Cavidade Pulpar , Dentina , Humanos , Irrigantes do Canal Radicular/química , Irrigantes do Canal Radicular/farmacologia , Preparo de Canal Radicular , Silicatos , Análise Espectral
6.
BMC Oral Health ; 22(1): 207, 2022 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-35614409

RESUMO

BACKGROUND: This study aimed to assess the effect of different commercially used calcium hydroxide [Ca(OH)2], the mixture of Ca(OH)2 + silver nanoparticles (AgNPs), and other intracanal medicaments on dislodgement resistance of mineral trioxide aggregate (MTA) to root canal dentin in short- and long-term application. METHODS: Forty-six human single-rooted maxillary teeth were sectioned horizontally at mid-root into 1 mm thick slices. The lumen of the slices was standardized using #2-#5 Gates Glidden drills to a standard diameter of 1.3 mm in all samples. After smear layer removal, the samples were randomly divided into eight groups (n = 20) and treated with the following medicaments; Ca(OH)2 paste, Calcipex, Metapex, chlorhexidine (CHX), Ca(OH)2/CHX paste, Ca(OH)2/AgNPs, triple antibiotic paste and control group (normal saline). The samples were then incubated at 37 °C with 100% humidity for 1 week. Next, half of the teeth in each group (n = 10) were removed from the incubator and washed in an ultrasonic bath. ProRoot MTA was placed in the canal lumen and the samples were incubated at 37 °C and 100% humidity for 48 h to allow complete setting of MTA. The remaining half in each group (n = 10) was subjected to the same process after 1 month of application of medicaments. The dislodgement resistance of MTA to root dentin was measured. The data were analysed using two-way ANOVA and Tukey's post hoc test. RESULTS: No significant difference was noted in dislodgement resistance of samples after 1 week and 1 month in any group (P > 0.05). The only significant difference was noted between the control and CHX groups and the higher dislodgement resistance was recorded in the CHX group (P = 0.006). No other significant differences were noted between the groups (P > 0.05). CONCLUSION: Duration of application and the type of intracanal medicament do not affect the dislodgement resistance of MTA to root dentin. Although there was no statistically significant difference in the dislodgment resistance of MTA between the medicaments, CHX had a promising effect.


Assuntos
Nanopartículas Metálicas , Compostos de Alumínio , Compostos de Cálcio , Hidróxido de Cálcio/farmacologia , Combinação de Medicamentos , Humanos , Óxidos , Pemetrexede , Irrigantes do Canal Radicular , Silicatos , Prata/farmacologia
8.
Braz Dent J ; 33(2): 1-11, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35508031

RESUMO

The aim was to assess the physicochemical properties and the penetration into dentinal tubules of calcium hypochlorite solution [Ca(OCl)2], with or without surfactants. The surfactants benzalkonium chloride, cetrimide, Tween 80 and Triton X-100 were mixed at different concentrations with sodium hypochlorite solution (NaOCl), Ca(OCl)2 and distilled water (control). Once the critical micellar concentration (CMC) of the surfactants in Ca(OCl)2 and NaOCl was determined, pH, free chlorine, surface tension and free calcium ions were evaluated. The penetration into dentinal tubules of NaOCl and Ca(OCl)2, with or without benzalkonium chloride and Triton X-100 [surfactants that promoted the lowest surface tension of Ca(OCl)2], was assessed using human premolars stained with crystal violet. The statistical tests were one-way ANOVA and Tukey's post-test, Kruskal-Wallis and Dunn's post-test, two-way ANOVA and Bonferroni's post-test, and t-test; depending on the assay. The addition of surfactants reduced the surface tension of NaOCl and Ca(OCl)2, and did not alter the pH or the free available chlorine of either solution. The addition of all surfactants increased the availability of free calcium ions in Ca(OCl)2, especially benzalkonium chloride. Ca(OCl)2 exhibited lower penetration into dentinal tubules than NaOCl, and the addition of surfactants did not improve the penetration of Ca(OCl)2, but did increase the penetration of NaOCl. It can be concluded that the addition of surfactants to Ca(OCl)2 did not increase the penetration into dentinal tubules, but it did promote lower surface tension, without changing the pH or free available chlorine values, and higher availability of free calcium ions in Ca(OCl)2.


Assuntos
Irrigantes do Canal Radicular , Hipoclorito de Sódio , Compostos de Benzalcônio , Cálcio , Compostos de Cálcio , Cloro , Humanos , Octoxinol , Irrigantes do Canal Radicular/química , Hipoclorito de Sódio/química , Tensoativos/química
9.
BMC Oral Health ; 22(1): 179, 2022 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-35568838

RESUMO

BACKGROUNDS: To compare the irrigation efficiency with different needle working length and different root canal curvature based on a real unshaped root canal using computational fluid dynamics (CFD) method. METHODS: Images of the root canal of the maxillary first molar after being prepared to .04/15 were scanned using micro-CT, and then imported into the software for three-dimensional reconstruction. A palatal root canal with a curvature of 23.4° was selected as the experiment canal. The needle working length of the 30-G flat needle was 4.75 mm, 5 mm, 5.25 mm and 5.5 mm short of apical foramen respectively, the flow pattern, irrigation velocity, shear stress were compared. The modified curved canals with a curvature of 0°, 5°, 10°, 20° and 30°were reconstructed via software. The flat needle was replaced at the optical inserted depth, and key parameters of irrigation efficiency were analyzed. RESULTS: Decreased needle working length had a positive impact on irrigation efficiency. With the optimal needle working length, the replacement of the apical irrigation fluid, the effective velocity, and wall shear stress were significantly improved in more severely curved root canals. With the same needle working depth and analogous canal curvature, irrigation efficiency is higher in real canal than that of modified canal. CONCLUSIONS: Short needle working depth, large curvature and the anomalous inner wall of canals help to improve irrigation efficiency.


Assuntos
Cavidade Pulpar , Hidrodinâmica , Cavidade Pulpar/diagnóstico por imagem , Humanos , Agulhas , Irrigantes do Canal Radicular , Preparo de Canal Radicular/métodos , Irrigação Terapêutica
10.
Shanghai Kou Qiang Yi Xue ; 31(1): 6-11, 2022 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-35587660

RESUMO

PURPOSE: To evaluate the safety and effectiveness of passive ultrasonic irrigation(PUI) in curved root canals, and debris cleaning ability and the amount of root canal transportation of passive ultrasonic irrigation(PUI) in curved root canals. METHODS: A total of 36 mesially curved root canals of mandibular molars with a curvature above 25° were selected. The root canals were prepared with XP-endo Shaper root canal file. The samples were divided into A1 group (curved length>3 mm, syringe irrigation), B1 group(curved length>3 mm, PUI+K file), C1 group(curved length>3 mm, PUI+irrisafe), A2 group (curved length<3 mm, syringe irrigation), B2 group (curved length<3 mm, PUI+K file), and C2 group (curved length<3 mm, PUI+ irrisafe) (n=6). Micro-CT scans were performed on all samples before and after irrigation, and the volume increase in the root canal after irrigation and the transportation of the root canal were calculated. SPSS 22.0 software package was used to analyze the data. RESULTS: In the apical area of the root canal with a curved length greater than 3 mm, the root canal volume increase in the PUI+irrisafe group was significantly greater than that of the PUI+K file and syringe irrigation (P<0.05), and at 5 mm section, the transportation of the root canal formed by PUI+irrisafe was significantly lower than that of the PUI+K file (P<0.05), but there was no significant difference from syringe irrigation (P>0.05); in root canals with a curved length less than 3 mm, root canal volume increment of the file group was significantly greater than that of syringe irrigation (P<0.05), but there was no significant difference between the root canal transportation and syringe irrigation(P<0.05). CONCLUSIONS: In root canals with larger curved length, passive ultrasonic irrigation combined with a pre-curved file can obtain a better cleaning effect, while in root canals with smaller curved length, both K file and pre-curved file with passive ultrasonic irrigation are safe and effective.


Assuntos
Cavidade Pulpar , Preparo de Canal Radicular , Cavidade Pulpar/diagnóstico por imagem , Dente Molar , Irrigantes do Canal Radicular/efeitos adversos , Preparo de Canal Radicular/efeitos adversos , Tratamento do Canal Radicular , Ultrassom
11.
Shanghai Kou Qiang Yi Xue ; 31(1): 17-23, 2022 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-35587662

RESUMO

PURPOSE: To obtain an efficient and simple root canal disinfection method based on minimally invasive root canal treatment by comparing different root canal disinfection methods between minimally invasive root canal treatment and conventional root canal treatment. METHODS: Sixty-six extracted maxillary first molars were randomly divided into experimental group (computer-guided precision minimally invasive root canal treatment) and control group (conventional root canal treatment). All teeth were prepared to ProTaper universal F2, and Enterococcus faecalis infection models were established.Each group was randomly divided into three subgroups, sodium hypochlorite+EDTA root canal irrigation, sodium hypochlorite+EDTA+ultrasonic and sodium hypochlorite +EDTA +Er: YAG laser. After root canal disinfection,the samples were collected by paper tip method and cultured, and colony forming units (CFU) values of each sample were calculated. Then dentin debris was prepared and collected with F3 file. After being diluted and cultured, the CFU value was calculated. Statistical analysis was performed by SPSS 26.0 software package. RESULTS: Comparison of the amount of bacterial inner wall of root canal between the experimental group and the control group showed that the germicidal efficacy of group C and group B were significantly better than that of group A (P<0.05), but there was no significant difference between group B and group C(P>0.05). In the experimental group, there was significant difference between group B1, C1 and A1 (P<0.05). The results of group B1 and C1 were lower than that of group A1, but there was no significant difference between group B1 and group C1(P>0.05). In the control group, there were significant differences between group B2, C2 and A2 (P<0.05). The results of group B2 and C2 were lower than that of group A2, but there was no significant difference between group B2 and C2(P>0.05). Comparison of the amount of bacteria in dentin debris between the experimental group and the control group showed that the effect of group C was the best, followed by group B, and group A, and there were significant differences between three groups(P<0.05). CONCLUSIONS: The disinfection effect of Er:YAG laser or ultrasound assisted computer-guided precision minimally invasive root canal treatment is similar to conventional root canal treatment, and Er:YAG laser is better than ultrasound in removing bacteria from dentinal tubules, which is more suitable for minimally invasive root canal treatment.


Assuntos
Enterococcus faecalis , Hipoclorito de Sódio , Cavidade Pulpar/microbiologia , Desinfecção/métodos , Ácido Edético , Irrigantes do Canal Radicular , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio/farmacologia
12.
BMC Oral Health ; 22(1): 201, 2022 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-35606751

RESUMO

BACKGROUND: To investigate the effect of a rotary agitation method or ultrasonically activated irrigation on the antibiofilm effect of a mixture of sodium hypochlorite (NaOCl) and etidronate (1-hydroxyethylidene-1,1-bisphosphonate, HEBP) using a dual-species biofilm model in root canal system. METHODS: Mature dual-species biofilms of Enterococcus faecalis and Streptococcus gordonii were formed in root canals of mandibular premolars. Teeth were randomly allotted (n = 12) to group 1, XP-endo Finisher (XPF); group 2, ultrasonically activated irrigation (UAI); group 3, syringe-and-needle irrigation (SNI). In all groups, canals were instrumented with a rotary instrument (XP-endo Shaper) prior to irrigant agitation/activation. A mixture containing 2.5% NaOCl and 9% HEBP was used throughout the experiment. Bacterial counts from the canal were determined using qPCR before preparation (S1), after preparation (S2), and after final irrigation agitation/activation (S3). Bacterial viability within the dentinal tubules in the coronal, middle and apical root-thirds was quantified using confocal microscopy after Live/Dead staining. The bacterial counts and viability were compared between groups using one-way ANOVA and post-hoc Tukey's tests. Paired t-test was used to compare the bacterial counts within groups. RESULTS: Instrumentation alone could significantly reduce the microbial counts in all the groups (P < 0.0001). Subsequent agitation/activation resulted in significant microbial reduction only in XPF and UAI (P < 0.05), both of which reduced significantly more microbial counts than SNI (P < 0.05). Live/Dead staining revealed that XPF and UAI showed significantly greater percentage of dead bacteria within the dentinal tubules than SNI in the coronal third (P < 0.05); UAI resulted in the significantly highest percentage of dead bacteria in the middle third (P < 0.05); while there was no significant difference between the groups in the apical third (P > 0.05). CONCLUSIONS: When using the sodium hypochlorite/etidronate mixture for irrigation, final irrigant agitation/activation with XP-endo Finisher or ultrasonic can improve disinfection of the main root canal space and the dentinal tubules in the coronal third, while ultrasonically activated irrigation appears to exhibit better disinfection within dentinal tubules in the middle third.


Assuntos
Ácido Etidrônico , Hipoclorito de Sódio , Biofilmes , Cavidade Pulpar/microbiologia , Ácido Etidrônico/farmacologia , Ácido Etidrônico/uso terapêutico , Humanos , Ácido Hipocloroso , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio/farmacologia , Irrigação Terapêutica
13.
Sci Rep ; 12(1): 7595, 2022 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-35534609

RESUMO

Calcium hydroxide Ca(OH)2 has been used as an intracanal medicament to targets microbial biofilms and avert secondary infection in the root canal system. This study evaluated the effects of this material on the morphology and physicochemical properties of an established in-vitro biofilm of Enterococcus faecalis. A biofilm of E. faecalis was grown in multichannel plates. The chemicals including Ca2+, OH-, and saturated Ca(OH)2 (ie 21.6 mM) were prepared in order to evaluate which component eradicated or amplified biofilm structure. Various biochemical and microscopic methods were used to investigate the properties of the biofilm. Biofilms treated with Ca(OH)2 absorbed more Ca2+ because of the alkaline pH of the environment and the ions affected the physicochemical properties of the E. faecalis biofilm. A denser biofilm with more cavities and a granular surface was observed in the presence of Ca2+ ions. This resulted in a decrease in the surface-to-biofilm ratio with increases in its biomass, thickness, colony size, and volume. Calcium hydroxide did not destroy E. faecalis biofilms but rather contributed to the biofilm structure. This in-vitro study sheds light on a missing link in the formation of E. faecalis biofilm in which the Ca2+ in Ca(OH)2.


Assuntos
Hidróxido de Cálcio , Enterococcus faecalis , Antibacterianos/farmacologia , Biofilmes , Hidróxido de Cálcio/farmacologia , Irrigantes do Canal Radicular/farmacologia , Tratamento do Canal Radicular
14.
BMC Oral Health ; 22(1): 119, 2022 04 09.
Artigo em Inglês | MEDLINE | ID: mdl-35397605

RESUMO

BACKGROUND: The main goal of an endodontic treatment is a complete debridement of the root canal system; however, currently mechanical shaping and chemical cleaning procedures for this purpose have deemed non-satisfactory. METHODS: The efficacy of peracetic acid (PAA; 0.5, 1.0, 2.0%), as a root canal irrigation solution, against Enterococcus faecalis (DSM 20478) and Parvimonas micra (DSM 20468) when compared with the one of sodium hypochlorite (NaOCI; 1.0, 3.0, 5.0%), chlorhexidine digluconate (CHX; 0.12, 0.2, 2.0%) and 0.9% NaCI (as a control solution) was in vitro investigated with the agar diffusion and direct contact methods. The inhibition zone diameters observed with the agar diffusion test were determined. The viable bacterial counts (CFU/ml) were calculated with the direct method. RESULTS: The agar diffusion test showed that all three root canal irrigation solutions had an efficacy against E. faecalis at all concentrations. The largest inhibition zone diameters against E. faecalis were observed with 5.0% NaOCI. At all three concentrations of PAA, NaOCI, and CHX, the inhibition zone diameter increased with increase in concentration. For P. micra, PAA had a similar inhibition zone diameter despite a concentration increase. In contrast, for NaOCI and CHX, the inhibition zone diameter increased with increasing concentration. 2.0% CHX produced the largest inhibition zone diameter against P. micra. For E. faecalis, only the comparison between 2.0% PAA and 5.0% NaOCI showed statistical significance (p = 0.004). For P. micra the efficacy comparison between the lowest, middle, and highest concentrations of each solution, a statistical significance (p < 0.05) was found for all three solutions. After direct contact with PAA, NaOCI and CHX, no viable bacteria could be determined for either P. micra or E. faecalis. CONCLUSIONS: PAA had a similar antibacterial efficacy as the one of NaOCl and CHX when in direct contact with E. faecalis and P. micra. In the agar diffusion test, PAA showed a similar antibacterial efficacy as the one of CHX and a lower one as the one of NaOCl for E. faecalis.


Assuntos
Enterococcus faecalis , Hipoclorito de Sódio , Ágar , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Clorexidina/farmacologia , Clorexidina/uso terapêutico , Cavidade Pulpar , Firmicutes , Humanos , Ácido Peracético/farmacologia , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/uso terapêutico , Hipoclorito de Sódio/farmacologia , Hipoclorito de Sódio/uso terapêutico
16.
Int Endod J ; 55(7): 758-771, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35470434

RESUMO

AIM: The addition of etidronic acid (HEDP) to sodium hypochlorite (NaOCl) could increase the antibiofilm potency of the irrigant, whilst maintaining the benefits of continuous chelation. Studies conducted so far have shown that mixing HEDP with NaOCl solutions of relatively low concentration does not compromise the antibiofilm efficacy of the irrigant. However, the working lifespan of NaOCl may decrease resulting in a reduction of its antibiofilm efficacy over time (efficiency). In this regard, continuous irrigant replenishment needs to be examined. This study investigated the response of a dual-species biofilm when challenged with 2% and 5% NaOCl mixed with HEDP for a prolonged timespan and under steady laminar flow. METHODOLOGY: Dual-species biofilms comprised of Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were grown on human dentine discs in a constant depth film fermenter (CDFF) for 96 h. Biofilms were treated with 2% and 5% NaOCl, alone or mixed with HEDP. Irrigants were applied under steady laminar flow for 8 min. Biofilm response was evaluated by means of optical coherence tomography (OCT). Biofilm removal, biofilm disruption, rate of biofilm loss and disruption as well as bubble formation were assessed. One-way anova, Wilcoxon's signed-rank test and Kruskal-Wallis H test were performed for statistical analysis of the data. The level of significance was set at a ≤.05. RESULTS: Increasing NaOCl concentration resulted in increased biofilm removal and disruption, higher rate of biofilm loss and disruption and increased bubble formation. Mixing HEDP with NaOCl caused a delay in the antibiofilm action of the latter, without compromising its antibiofilm efficacy. CONCLUSIONS: NaOCl concentration dictates the biofilm response irrespective of the presence of HEDP. The addition of HEDP resulted in a delay in the antibiofilm action of NaOCl. This delay affects the efficiency, but not the efficacy of the irrigant over time.


Assuntos
Ácido Etidrônico , Irrigantes do Canal Radicular , Biofilmes , Humanos , Irrigantes do Canal Radicular/farmacologia , Hipoclorito de Sódio/farmacologia , Tomografia de Coerência Óptica
17.
ACS Appl Bio Mater ; 5(5): 2135-2142, 2022 05 16.
Artigo em Inglês | MEDLINE | ID: mdl-35476392

RESUMO

Conventional methods used to control bacterial biofilm infection in root canals have poor efficacy, causing repeated and chronic infections, which pose a great challenge to clinical treatment. Microbubbles, due to their small size and ultrasound (US)-enhanced cavitation effects, have attracted considerable clinical attention. They possess the potential for therapeutic application in restricted spaces. We address the above problem with a strategy for the restricted space of root canals. Herein, phase-change nanodroplets (P-NDs) exposed to US are combined with common antibacterial drugs to disrupt a 7 day Enterococcus faecalis biofilm in an in vitro human tooth model. Specifically, the preparation of P-NDs is based on secondary cavitation. Their average particle size is ∼144 nm, and the stability is favorable. The clearance effect for the biofilm is notable (the disruption rate of P-NDs + US is 63.1%, P < 0.01), while the effect of an antibacterial in conjunction with 2% chlorhexidine (Chx) is significant (the antibiofilm rate of P-NDs@2% Chx + US is 96.2%, P < 0.001). Furthermore, biocompatibility testing on human periodontal ligament fibroblasts demonstrated that P-NDs are safe. In summary, the strategy that we have proposed is suitable for the removal of biofilms in root canals. Notably, it also has great potential for application in the treatment of bacterial infections in restricted spaces.


Assuntos
Enterococcus faecalis , Irrigantes do Canal Radicular , Antibacterianos/farmacologia , Biofilmes , Clorexidina/farmacologia , Cavidade Pulpar , Humanos , Irrigantes do Canal Radicular/farmacologia
18.
Sci Rep ; 12(1): 6354, 2022 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-35428859

RESUMO

Silane-based/fully hydrolyzed, endodontic irrigant exhibiting antimicrobial properties, is prepared, and is hypothesized to control macrophage polarization for tissue repair. Albino wistar rats were injected with 0.1 ml root canal irrigant, and bone marrow cells procured. Cellular mitochondria were stained with MitoTracker green along with Transmission Electron Microscopy (TEM) performed for macrophage extracellular vesicle. Bone marrow stromal cells (BMSCs) were induced for M1 and M2 polarization and Raman spectroscopy with scratch assay performed. Cell counting was used to measure cytotoxicity, and fluorescence microscopy performed for CD163. Scanning Electron Microscopy (SEM) was used to investigate interaction of irrigants with Enterococcus faecalis. K21 specimens exhibited reduction in epithelium thickness and more mitochondrial mass. EVs showed differences between all groups with decrease and increase in IL-6 and IL-10 respectively. 0.5%k21 enhanced wound healing with more fibroblastic growth inside scratch analysis along with increased inflammation-related genes (ICAM-1, CXCL10, CXCL11, VCAM-1, CCL2, and CXCL8; tissue remodelling-related genes, collagen 1, EGFR and TIMP-2 in q-PCR analysis. Sharp bands at 1643 cm-1 existed in all with variable intensities. 0.5%k21 had a survival rate of BMSCs comparable to control group. Bacteria treated with 0.5%k21/1%k21, displayed damage. Antimicrobial and reparative efficacy of k21 disinfectant is a proof of concept for enhanced killing of bacteria across root dentin acquiring functional type M2 polarization for ethnopharmacological effects.


Assuntos
Anti-Infecciosos , Silanos , Animais , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Biofilmes , Dentina , Enterococcus faecalis , Macrófagos , Modelos Animais , Irrigantes do Canal Radicular/farmacologia , Silanos/farmacologia , Hipoclorito de Sódio/farmacologia
19.
J Endod ; 48(6): 768-774, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35247369

RESUMO

INTRODUCTION: This study evaluated the effectiveness of the GentleWave System (GWS; Sonendo, Laguna Hills, CA) and passive ultrasonic irrigation (PUI) in removing Enterococcus faecalis lipoteichoic acid (LTA) from infected root canals with a minimally invasive technique (MIT) and the conventional instrumentation technique (CIT). METHODS: Sixty extracted human maxillary first premolars were included. All teeth were dentin pretreated and inoculated with LTA prepared from E. faecalis. First, 12 teeth were cryogenically ground to investigate the viability of recovering intraradicular E. faecalis LTA. Afterward, 48 teeth were randomly divided into the following groups: GWS + MIT, GWS + CIT, PUI + MIT, and GWS + CIT (all n = 12). Teeth were instrumented with a Vortex Blue (Dentsply Sirona, Ballaigues, Switzerland) rotary file size 15/.04 for MIT and 35/.04 for CIT. Samples were collected before and after a root canal procedure with sterile/apyrogenic paper points and after cryogenically ground for intraradicular LTA analysis. LTA was quantified with an LTA enzyme-linked immunosorbent assay kit. RESULTS: E. faecalis LTA was recovered from 100% of the samples (48/48) before the root canal procedure. GWS + MIT and GWS + CIT were the most effective protocols against E. faecalis LTA, with no difference between them (P > .05). PUI + CIT was more effective than PUI + MIT (P < .05) but less effective than GWS + MIT and GWS + CIT (P < .05). The GWS groups showed more root canals with undetected E. faecalis LTA after treatment than all groups tested. CONCLUSIONS: GWS + MIT and GWS + CIT were the most effective protocols against E. faecalis LTA in infected root canals.


Assuntos
Cavidade Pulpar , Enterococcus faecalis , Humanos , Lipopolissacarídeos , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular , Hipoclorito de Sódio , Ácidos Teicoicos , Ultrassom
20.
J Endod ; 48(6): 775-780, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35314292

RESUMO

INTRODUCTION: Lateral canals are particularly challenging to clean and disinfect. The aim of this study was to compare the removal efficacy of a dual-species biofilm from a lateral canal model by different ultrasonic irrigant activation protocols in vitro. METHODS: Artificial root canal models with 270 simulated lateral canals were made of polydimethylsiloxane. A dual-species biofilm (Streptococcus oralis and Actinomyces naeslundi) was grown in vitro in the lateral canals using a constant depth film fermenter. Two percent NaOCl or demineralized water was delivered by a syringe and an open-ended needle for 30 seconds and subsequently activated by an ultrasonic file for a total activation time of 30, 60, or 90 seconds divided in 1 or 3 consecutive activation cycles. In the control groups, the irrigant was allowed to rest for 30, 60, or 90 seconds. The volume of the biofilm in the lateral canal was evaluated before and after the final irrigation protocol by optical coherence tomography. The results were analyzed by 3-way factorial analysis of variance (α = 0.05). RESULTS: Irrigation with NaOCl rather than demineralized water resulted in more effective biofilm removal from the lateral canal (P < .001). Three cycles of intermittent ultrasonic activation were significantly more effective than no activation (P = .029). The total irrigant contact time did not affect biofilm removal (P = .403). CONCLUSIONS: The type of the irrigant and the ultrasonic activation protocol affected biofilm removal from artificial lateral canals. None of the compared protocols was able to eradicate the biofilm.


Assuntos
Cavidade Pulpar , Irrigantes do Canal Radicular , Biofilmes , Irrigantes do Canal Radicular/farmacologia , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio/farmacologia , Irrigação Terapêutica/métodos , Ultrassom , Água
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...