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1.
BMJ Case Rep ; 13(12)2020 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-33370932

RESUMO

A 58-year-old woman presented with a 1-week history of lower limb bruising. She had a medical history of recurrent metastatic colon cancer with a sigmoid colectomy and complete pelvic exenteration leading to colostomy and urostomy formation. She had malignant sacral mass encroaching on the spinal cord. This caused a left-sided foot drop for which she used an ankle-foot orthosis. She was on cetuximab and had received radiotherapy to the sacral mass 1 month ago. On examination, there were macular ecchymoses with petechiae on the lower limbs. There was sparing of areas that had been compressed by the ankle-foot orthosis. Bloods showed mild thrombocytopaenia and anaemia with markedly raised inflammatory markers. Coagulation studies consistent with inflammation rather than disseminated intravascular coagulation. She was found to have Klebsiella bacteraemia secondary to urinary source. Skin biopsy showed dermal haemorrhage without vessel inflammation. Vitamin C levels were low confirming the diagnosis of scurvy.


Assuntos
Ácido Ascórbico , Colectomia/efeitos adversos , Neoplasias Colorretais , Equimose , Desnutrição , Apoio Nutricional/métodos , Escorbuto , Antineoplásicos Imunológicos/uso terapêutico , Ácido Ascórbico/administração & dosagem , Ácido Ascórbico/sangue , Bacteriemia/diagnóstico , Bacteriemia/microbiologia , Colectomia/métodos , Neoplasias Colorretais/patologia , Neoplasias Colorretais/fisiopatologia , Neoplasias Colorretais/secundário , Neoplasias Colorretais/terapia , Diagnóstico Diferencial , Equimose/sangue , Equimose/diagnóstico , Equimose/etiologia , Feminino , Humanos , Klebsiella/isolamento & purificação , Extremidade Inferior , Desnutrição/etiologia , Desnutrição/terapia , Pessoa de Meia-Idade , Metástase Neoplásica , Estadiamento de Neoplasias , Exenteração Pélvica/efeitos adversos , Exenteração Pélvica/métodos , Escorbuto/sangue , Escorbuto/etiologia , Escorbuto/fisiopatologia , Escorbuto/terapia , Pele/patologia , Resultado do Tratamento , Vitaminas/administração & dosagem
2.
PLoS One ; 15(6): e0235391, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32603368

RESUMO

BACKGROUND: Neonatal septicemia is a life threatening medical emergency that requires timely detection of pathogens with urgent rational antibiotics therapy. METHODS: A cross-sectional study was conducted between March 2017 to September 2018 among 317 septicemia suspected neonates at neonatal intensive care unit, Ayder Comprehensive Specialized Hospital, Mekelle, Tigray, North Ethiopia. A 3 mL of blood was collected from each participant. Identification of bacterial species was done using the standard microbiological techniques. Antibiotic sensitivity test was done using disk diffusion method. Data were entered and analyzed using computer software SPSS version 22. Bivariate and multivariate regression analysis was applied to determine the association between variables. RESULTS: Of the 317 (190 male and 127 female) neonates, 116 (36.6%) were found to be with culture proven septicemia. Klebsiella species were the predominant etiologic agents. Length of hospital stay (AOR (adjusted odds ratio) = 3.65 (2.17-6.13), p < 0.001) and low birth weight (AOR = 1.64 (1.13-2.78), p = 0.04) were the factors associated with neonatalsepticemia. Most isolates showeda frightening drug resistance rate to the commonly used antimicrobial drugs. K. pneumoniae, E. coli, Enterobacter and Citrobacter species were 57% to100% resistant to ceftazidime, ceftriaxone, gentamycin, amoxacillin-clavulunic acid and ampicillin. All, 9 (100%) isolates of S. aureus were resistant to oxacilline, ampicillin,erythromycin and gentamycin. Furthermore, 55.6% S. aureus isolates were Methicillin Resistant Staphylococcus aureus. CONCLUSION: Neonaltal septicemia is found to be significantly high in the present study. As most of the isolates are potentially related to hospital acquired infections, prevention and control policy should have to be more strengthening in the neonatal intensive care unit.


Assuntos
Antibacterianos/uso terapêutico , Bactérias , Sepse Neonatal , Ampicilina/uso terapêutico , Bactérias/classificação , Bactérias/efeitos dos fármacos , Bactérias/isolamento & purificação , Citrobacter/efeitos dos fármacos , Citrobacter/isolamento & purificação , Estudos Transversais , Farmacorresistência Bacteriana , Enterobacter/efeitos dos fármacos , Enterobacter/isolamento & purificação , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Etiópia , Feminino , Humanos , Recém-Nascido , Unidades de Terapia Intensiva Neonatal , Klebsiella/efeitos dos fármacos , Klebsiella/isolamento & purificação , Masculino , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Testes de Sensibilidade Microbiana , Sepse Neonatal/tratamento farmacológico , Sepse Neonatal/microbiologia , Oxacilina/uso terapêutico , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/isolamento & purificação
3.
Ecotoxicol Environ Saf ; 201: 110804, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32502907

RESUMO

Phenanthrene (PHE) is harmful to human health and is difficult to be eliminated from environment. In this study, an aerobic bacterium capable of use PHE as a sole carbon source and energy was isolated and classified as Klebsiella sp. PD3 according to 16S rDNA analysis. The degradation efficiency of PHE reached to about 78.6% after 12 days of incubation with strain PD3. Identification of metabolites formed during PHE degradation process by this strain was carried out by GC-MS. The first degradation step of PHE by PD3 was proposed to generate 1-hydroxy-2-naphthoic acid. Two subsequent different routes for the metabolism of 1-hydroxy-2-naphthoic acid were proposed. Strain PD3 also showed two plant growth promoting properties like phosphate solubilization and ACC deaminase activity. Inoculation with Klebsiella sp. PD3 significantly improved growth performance, biomass production, seed germination rate, photosynthetic capacity, antioxidant levels, relative water content and chlorophyll accumulation in rice (Oryza sativa L.) plants under PHE stress conditions in comparison with non-inoculation treatment. Moreover, PD3-inoculated rice showed lower ROS accumulation, ethylene production, ACC content, ACC oxidase activity and electrolyte leakage under PHE treatment compared to non-inoculated ones. The combination use of rice plants and strain PD3 was also shown to enhance the removal efficiency of PHE from the soil and decline the PHE accumulation in plants. Synergistic use of plants and bacteria with PHE degradation ability and PGPR attributes to remediate the PHE-contaminated soil will be an important and effective way in the phytoremediation of PHE-contaminated soils.


Assuntos
Adaptação Fisiológica , Klebsiella/metabolismo , Oryza/crescimento & desenvolvimento , Fenantrenos/análise , Microbiologia do Solo , Poluentes do Solo/análise , Biodegradação Ambiental , Clorofila/metabolismo , Etilenos/metabolismo , Klebsiella/isolamento & purificação , Oryza/metabolismo , Oryza/microbiologia , Estresse Oxidativo/efeitos dos fármacos , Fenantrenos/metabolismo , Solo/química , Poluentes do Solo/metabolismo
4.
PLoS One ; 15(6): e0234558, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32584852

RESUMO

Recently, our understanding of the elusive bacterial communities in the lower respiratory tract and their role in chronic lung disease has increased significantly. However, little is known about the respiratory microorganisms in patients with endobronchial tuberculosis (EBTB), which is a chronic inflammatory disease characterized by destruction of the tracheobronchial tree due to Mycobacterium tuberculosis (MTB) infection. We retrospectively reviewed data for histopathologically and microbiologically confirmed EBTB patients diagnosed at a tertiary referral hospital in South Korea between January 2013 and January 2019. Bacterial cultures were performed on bronchial washing from these patients at the time of EBTB diagnosis. A total of 216 patients with EBTB were included in the study. The median age was 73 years and 142 (65.7%) patients were female. Bacteria were detected in 42 (19.4%) patients. Additionally, bacterial co-infection was present in 6 (2.8%) patients. Apart from MTB, the most common microorganisms identified were Staphylococcus aureus (n = 14, 33.3%) followed by Klebsiella species (n = 12, 28.6%; 10 Klebsiella pneumoniae, 2 Klebsiella oxytoca), Streptococcus species (n = 5, 11.9%), Enterobacter species (n = 4, 9.5%), and Pseudomonas aeruginosa (n = 3, 7.1%). A variety of microorganisms were isolated from the bronchial washing indicating that changes in microorganism composition occur in the airways of patients with EBTB. Further studies are needed to investigate the clinical significance of this finding.


Assuntos
Sistema Respiratório/microbiologia , Tuberculose Pulmonar/microbiologia , Idoso , Broncoscopia , Enterobacter/isolamento & purificação , Feminino , Humanos , Klebsiella/isolamento & purificação , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/isolamento & purificação , Pseudomonas aeruginosa/isolamento & purificação , República da Coreia , Estudos Retrospectivos , Staphylococcus/isolamento & purificação
5.
Appl Environ Microbiol ; 86(16)2020 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-32503911

RESUMO

Traditional fermentations have been widely studied from the microbiological point of view, but little is known from the functional perspective. In this work, nitrogen fixation by free-living nitrogen-fixing bacteria was conclusively demonstrated in pozol, a traditional Mayan beverage prepared with nixtamalized and fermented maize dough. Three aspects of nitrogen fixation were investigated to ensure that fixation actually happens in the dough: (i) the detection of acetylene reduction activity directly in the substrate, (ii) the presence of potential diazotrophs, and (iii) an in situ increase in acetylene reduction by inoculation with one of the microorganisms isolated from the dough. Three genera were identified by sequencing the 16S rRNA and nifH genes as Kosakonia, Klebsiella, and Enterobacter, and their ability to fix nitrogen was confirmed.IMPORTANCE Nitrogen-fixing bacteria are found in different niches, as symbionts in plants, in the intestinal microbiome of several insects, and as free-living microorganisms. Their use in agriculture for plant growth promotion via biological nitrogen fixation has been extensively reported. This work demonstrates the ecological and functional importance that these bacteria can have in food fermentations, reevaluating the presence of these genera as an element that enriches the nutritional value of the dough.


Assuntos
Acetileno/metabolismo , Bactérias/metabolismo , Enterobacteriaceae/metabolismo , Alimentos e Bebidas Fermentados/microbiologia , Fixação de Nitrogênio , Enterobacter/isolamento & purificação , Enterobacter/metabolismo , Enterobacteriaceae/isolamento & purificação , Klebsiella/isolamento & purificação , Klebsiella/metabolismo , México , Oxirredução , Oxirredutases/análise , RNA Bacteriano/análise , RNA Ribossômico 16S/análise
6.
Int J Syst Evol Microbiol ; 70(5): 3278-3286, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32375936

RESUMO

A novel bacterial strain, designated TOUT106T, was isolated from the surface of a tomato. The cells were rod-shaped, Gram-negative, encapsulated and non-motile. Strain TOUT106T grows best at 28 °C and pH 7.0 and can tolerate up to 2 % (w/v) NaCl. Based on 16S rRNA gene phylogeny, strain TOUT106T was placed close to the Salmonella clade, with close similarity to Salmonella enterica subsp. arizonae strain NCTC 8297T (98.42 %). Results of genome-based phylogenetic analysis revealed that strain TOUT106T is placed well in the Klebsiella-Raoultella clade, by forming a distinct branch with Klebsiella michiganensis DSM25444T, Klebsiella oxytoca NCTC132727T, Klebsiella grimontii 06D021T and Klebsiella pasteurii SB6412T. The genomic DNA G+C content of strain TOUT106T is 53.53 mol%. The average nucleotide identity values of TOUT106T were less than 86.5 % with closely related members of the family Enterobacteriaceae. The major fatty acids of strain TOUT106T were C16 : 0, C17:0 cyclo, C14:0 3OH/C16:1 iso, C14 : 0, C19:0 cyclo ω8c, C18:1 ω6c/C18:1 ω7c, C12 : 0 and C16:1 ω7c/C16:1 ω6c. Strain TOUT106T showed differences in physiological, phenotypic and protein profiles by MALDI-TOF MS compared to its closest relatives. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain TOUT106T could be distinguished from the recognized species of the genus Klebsiella. It is suggested to represent a novel species of this genus, for which the name Klebsiella indica sp. nov. is proposed. The type strain is TOUT106T (=MCC 2901T=KACC 21384T=JCM 33718T).


Assuntos
Frutas/microbiologia , Klebsiella/classificação , Lycopersicon esculentum/microbiologia , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Índia , Klebsiella/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
7.
PLoS Pathog ; 16(4): e1008441, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32294136

RESUMO

Gut symbiotic bacteria have a substantial impact on host physiology and ecology. However, the contribution of gut microbes to host fitness during long-term low-temperature stress is still unclear. This study examined the role of gut microbiota in host low-temperature stress resistance at molecular and biochemical levels in the oriental fruit fly Bactrocera dorsalis. The results showed that after the gut bacteria of flies were removed via antibiotic treatment, the median survival time was significantly decreased to approximately 68% of that in conventional flies following exposure to a temperature stress of 10°C. Furthermore, we found that Klebsiella michiganensis BD177 is a key symbiotic bacterium, whose recolonization in antibiotic treated (ABX) flies significantly extended the median survival time to 160% of that in the ABX control, and restored their lifespan to the level of conventional flies. Notably, the relative levels of proline and arginine metabolites were significantly downregulated by 34- and 10-fold, respectively, in ABX flies compared with those in the hemolymph of conventional flies after exposure to a temperature stress of 10°C whereas recolonization of ABX flies by K. michiganensis BD177 significantly upregulated the levels of proline and arginine by 13- and 10- fold, respectively, compared with those found in the hemolymph of ABX flies. qPCR analysis also confirmed that K. michiganensis-recolonized flies significantly stimulated the expression of transcripts from the arginine and proline metabolism pathway compared with the ABX controls, and RNAi mediated silencing of two key genes Pro-C and ASS significantly reduced the survival time of conventional flies, postexposure low-temperature stress. We show that microinjection of L-arginine and L-proline into ABX flies significantly increased their survival time following exposure to temperature stress of 10°C. Transmission electron microscopy (TEM) analysis further revealed that low-temperature stress caused severe destruction in cristae structures and thus resulted in abnormal circular shapes of mitochondria in ABX flies gut, while the recolonization of live K. michiganensis helped the ABX flies to maintain mitochondrial functionality to a normal status, which is important for the arginine and proline induction. Our results suggest that gut microbiota plays a vital role in promoting the host resistance to low-temperature stress in B. dorsalis by stimulating its arginine and proline metabolism pathway.


Assuntos
Arginina/metabolismo , Microbioma Gastrointestinal , Prolina/metabolismo , Tephritidae/microbiologia , Animais , Temperatura Baixa , Klebsiella/genética , Klebsiella/crescimento & desenvolvimento , Klebsiella/isolamento & purificação , Klebsiella/fisiologia , Masculino , Estresse Fisiológico , Simbiose , Tephritidae/fisiologia
8.
Diagn Microbiol Infect Dis ; 97(1): 114998, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32139114

RESUMO

There is limited data on the gut colonization rate of colistin resistant (Col-R) bacteria in patients and healthy volunteers in India. Aim of this study was to investigate the stool carriage rate of Col-R in hospitalized patients. Stool samples were inoculated in Eosin Methylene Blue agar plates supplemented with colistin. Colistin minimum inhibitory concentrations (MICs) were determined by the broth microdilution method. PCR for the mcr-1 was performed on Col-R Enterobacteriaceae isolates. Mutations in the mgrB gene were analyzed in K. pneumoniae isolates. Mcr-1 positive E. coli was subjected to whole-genome sequencing. Out of 65 stool samples screened, 33 (51%) samples carried Col-R bacteria. Majority (76.7%) of the isolates were sensitive to carbapenem.


Assuntos
Antibacterianos/farmacologia , Portador Sadio/microbiologia , Colistina/farmacologia , Enterobacteriaceae/efeitos dos fármacos , Fezes/microbiologia , Intestinos/microbiologia , Adulto , Carbapenêmicos/farmacologia , Farmacorresistência Bacteriana , Enterobacteriaceae/classificação , Enterobacteriaceae/fisiologia , Infecções por Enterobacteriaceae/microbiologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/genética , Escherichia coli/isolamento & purificação , Proteínas de Escherichia coli/genética , Feminino , Hospitalização , Humanos , Índia , Klebsiella/efeitos dos fármacos , Klebsiella/genética , Klebsiella/isolamento & purificação , Masculino , Testes de Sensibilidade Microbiana , Mutação
9.
J Med Microbiol ; 69(3): 396-401, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32125266

RESUMO

Introduction. Transmission of Enterobacterales in neonatal intensive care units (NICU) can cause outbreaks of colonization and invasive infections among neonates. Two clusters of nosocomial transmission of Klebsiella pneumoniae identified by MALDI-ToF mass-spectrometry were suspected at two NICUs in July and August 2016.Aim. To assess the potential transmission of K. pneumoniae among neonates.Methodology. Whole-genome sequencing (WGS) was performed of K. pneumoniae isolates obtained through targeted surveillance of patients and environmental sampling.Results. WGS data revealed that patient and environmental isolates represented two species, K. pneumoniae and K. variicola. Core-genome multi-locus sequence typing (cgMLST) of the isolates identified three separate transmission clusters, in Hospital A a cluster of K. pneumoniae isolates in 12 children and two environmental samples and a second cluster of K. variicola isolates in five children. In Hospital B a cluster of K. pneumoniae isolates from three children and five unrelated isolates of K. pneumoniae and two unrelated isolates of K. variicola were found.Conclusion. K. variicola can cause hospital outbreaks of colonization and infection similar to other Klebsiella spp.Preliminary results from this study were presented at the 27th European Congress of Clinical Microbiology and Infectious Diseases, April 22-25, 2018, Vienna, Austria.


Assuntos
Infecção Hospitalar , Surtos de Doenças , Genoma Bacteriano/genética , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/genética , Klebsiella/genética , Técnicas de Tipagem Bacteriana , Alemanha/epidemiologia , Humanos , Recém-Nascido , Unidades de Terapia Intensiva Neonatal , Klebsiella/efeitos dos fármacos , Klebsiella/isolamento & purificação , Infecções por Klebsiella/microbiologia , Infecções por Klebsiella/transmissão , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/isolamento & purificação , Tipagem de Sequências Multilocus , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Sequenciamento Completo do Genoma
10.
J Dairy Sci ; 103(4): 3392-3400, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32089315

RESUMO

Klebsiella spp. are important opportunistic pathogens commonly defined as environmental clinical mastitis agents. Despite Klebsiella mastitis being clinically impairing in cows and costly to the industry, only a few studies describe Klebsiella isolated from mastitis cases. The aim of this work was to characterize species of Klebsiella involved in clinical mastitis cases in Canada. Klebsiella isolated from clinical mastitis cases (n = 53) were identified to the species level using a biochemical test panel and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The rpoB gene sequence was used as the gold standard method and identified Klebsiella pneumoniae (n = 40), Klebsiella oxytoca (n = 9), Raoultella ornithinolytica (n = 2), and Raoultella planticola (n = 2). Raoultella, a genus closely related to Klebsiella, was also accurately identified using mass spectrometry but not via biochemical testing. Using the disc diffusion technique, 31 (58%) isolates were found to be susceptible to all antimicrobials tested (n = 18). The remaining 22 (42%) isolates were resistant to 1 or more of the following antimicrobials: kanamycin (2%), streptomycin (38%), spectinomycin (13%), sulfisoxazole (13%), and tetracycline (19%). The following antimicrobial resistance genes were identified: tetA, tetB, sul1, strA/strB, and aadA. Random amplified polymorphic DNA revealed the majority of our isolates as unrelated and having different patterns, indicating environmental contamination as the primary source of infection. All isolates were shown to be biofilm producers. In conclusion, although antimicrobial resistance was low for both Klebsiella and Raoultella species, genetically related Klebsiella spp. isolates appeared to be more resistant.


Assuntos
Klebsiella/isolamento & purificação , Mastite Bovina/microbiologia , Animais , Antibacterianos/farmacologia , Canadá , Bovinos , Farmacorresistência Bacteriana/genética , Feminino , Klebsiella/classificação , Klebsiella/efeitos dos fármacos , Klebsiella/fisiologia , Mastite Bovina/tratamento farmacológico , Testes de Sensibilidade Microbiana
11.
Microb Pathog ; 140: 103966, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31911192

RESUMO

Replacing agrochemicals with plant growth promoting bacteria (PGPB) may offset some of the environmental impacts of food production. The objectives of this study were to (1) isolate and characterize bacterial strains from tomato rhizosphere, including root, shoot and leaf, (2) select and identify the most promising PGPB strains, (3) verify the phytostimulatory activity and mineral uptake potential of selected strains. Bacterial strains isolated from tomato rhizosphere, were screened for phosphorous (P) solubilization, production of indole acetic acid (IAA), amylase activity, antibiotic resistance, and quick test strip (QTS) for biochemical characterization. The tested strains, positive for all five of these assays were selected for molecular identification and subjected to greenhouse growth trails with tomato and mung bean. Two strains were selected and identified as Bacillus cereus (B. cereus) isolated from rhizosphere and Klebsiella variicola (K. variicola) isolated from root endosphere using 16s rRNA sequences. Both strains produced IAA, gibberellic acid (GA3) and kinetin, however B. cereus showed potential GA3 and IAA production as compared to K. variicola. In tomato, only one growth variable (shoot length) was increased over the control by one of the selected bacterial strains (B. cereus). In mung bean, inoculation with either strain B. cereus or K. variicola increased shoot length and dry weight. Moreover, our results showed that the use of PGPB significantly increased plant growth and Fe, Zn, Ca, Mg, Cu, Na and K contents of plants. It seems that evaluated strains had a higher ability in boosting plant growth and higher yield.


Assuntos
Bactérias/isolamento & purificação , Lycopersicon esculentum/crescimento & desenvolvimento , Lycopersicon esculentum/microbiologia , Microbiologia do Solo , Bacillus cereus/classificação , Bacillus cereus/genética , Bacillus cereus/isolamento & purificação , Bacillus cereus/metabolismo , Bactérias/classificação , Bactérias/genética , Bactérias/metabolismo , Giberelinas/metabolismo , Ácidos Indolacéticos/metabolismo , Klebsiella/classificação , Klebsiella/genética , Klebsiella/isolamento & purificação , Klebsiella/metabolismo , Filogenia , Folhas de Planta/microbiologia , Raízes de Plantas/microbiologia , Rizosfera
12.
BMC Res Notes ; 12(1): 807, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31842990

RESUMO

OBJECTIVE: Edema syndrome is highly prevalent but under researched in captive frogs around the world. The objective of the present study was to characterize at a basic microbiological and cytological level of the bacteria of the edema fluid of 20 individuals of the genus Gastrotheca to determine the presence of possible anaerobic and aerobic bacteria. RESULTS: Fourteen types of bacteria were identified in the edema fluid, 12 of them at the species level (Pasteurella haemolytica, Hafnia alvei, Enterobacter agglomerans, Aeromonas hydrophila, Pseudomonas fluorescens, Burkholderia pseudomallei, Salmonella arizonae, Enterobacter gergoviae, Enterobacter sakazakii, Yersinia enterocolitica, Klebsiella oxytoca, and Klebsiella ozaenae) and two at the genus level (Enterococcus spp. and Streptococcus spp.). The most frequently identified cells were lymphocytes (37.7% in females and 46.4% in males), erythrocytes (23.5% in females and 17.5% in males) and neutrophils (4.2% in females and 2.8% in males). Finally, no relationship was found between the data obtained and the sex of the individuals studied.


Assuntos
Anuros/microbiologia , Edema/veterinária , Animais , Bactérias/isolamento & purificação , Burkholderia pseudomallei/isolamento & purificação , Edema/microbiologia , Espécies em Perigo de Extinção , Enterococcus/isolamento & purificação , Eritrócitos/citologia , Feminino , Klebsiella/isolamento & purificação , Linfócitos/citologia , Masculino , Neutrófilos/citologia
13.
BMC Infect Dis ; 19(1): 946, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31703559

RESUMO

BACKGROUND: Klebsiella variicola and K. quasipneumoniae are new species distinguishable from K. pneumoniae but they are often misidentified as K. pneumoniae in clinical settings. Several reports have demonstrated the possibility that the virulence factors and clinical features differ among these three phylogroups. In this study, we aimed to clarify whether there were differences in clinical and bacterial features between the three phylogroups isolated from patients with bloodstream infections (BSIs) in Japan. METHODS: Isolates from all patients with BSIs caused by K. pneumoniae admitted to two hospitals between 2014 and 2017 (n = 119) were included in the study. Bacterial species were identified via sequence analysis, and their virulence factors and serotypes were analyzed via multiplex PCR results. Clinical data were retrieved from medical records. RESULTS: Of the 119 isolates, 21 (17.7%) were identified as K. variicola and 11 (9.2%) as K. quasipneumoniae; K1 serotype was found in 16 (13.4%), and K2 serotype in 13 (10.9%). Significant differences in the prevalence of rmpA, iutA, ybtS, entB and kfu (p < 0.001), and allS genes (p < 0.05) were found between the three phylogroups. However, there were no significant differences in clinical features, including the 30-day mortality rate, between the three organisms, although K. variicola was more frequently detected in patients over 80 years old compared with other Klebsiella species (p < 0.005), and K. quasipneumoniae more frequently occurred in patients with malignancy (p < 0.05). CONCLUSIONS: Our findings demonstrated the differences in bacterial pathogenicity and clinical features among these three phylogroups. Further epidemiological studies into BSI caused by Klebsiella species are warranted.


Assuntos
Bacteriemia/microbiologia , Infecções por Klebsiella/microbiologia , Infecções por Klebsiella/mortalidade , Klebsiella pneumoniae/genética , Klebsiella/genética , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Doença Iatrogênica , Japão , Klebsiella/isolamento & purificação , Klebsiella pneumoniae/isolamento & purificação , Masculino , Filogenia , Reação em Cadeia da Polimerase , Estudos Retrospectivos , Fatores de Risco , Sorogrupo , Fatores de Virulência/genética
14.
J Dairy Sci ; 102(12): 11384-11400, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31606215

RESUMO

Objectives of this study were to (1) describe the intramammary infection (IMI) prevalence and pathogen profiles in quarters of cows approaching dry-off in US dairy herds, (2) compare IMI prevalence in quarters of cows exposed to different bedding material types, and (3) identify associations between bedding bacteria count and IMI in cows approaching dry-off. Eighty herds using 1 of 4 common bedding materials (manure solids, organic non-manure, new sand, and recycled sand) were recruited in a multi-site cross-sectional study. Each herd was visited twice for sampling. At each visit, aseptic quarter-milk samples were collected from 20 cows approaching dry-off (>180 d pregnant). Samples of unused and used bedding were also collected. Aerobic culture was used to determine the IMI status of 10,448 quarters and to enumerate counts (log10 cfu/mL) of all bacteria, Staphylococcus spp., Streptococcus spp. and Streptococcus-like organisms (SSLO), coliforms, Klebsiella spp., noncoliform gram-negatives, Bacillus spp., and Prototheca spp. in unused (n = 148) and used (n = 150) bedding. The association between bedding bacteria count and IMI was determined using multivariable logistic regression with mixed effects. Quarter-level prevalence of IMI was 21.1%, which was primarily caused by non-aureus Staphylococcus spp. (11.4%) and SSLO (5.6%). Only modest differences in IMI prevalence were observed between the 4 common bedding material types. Counts of all bacteria in unused bedding was positively associated with odds of IMI caused by any pathogen [ALL-IMI; odds ratio (OR) = 1.08]. A positive association was also observed for counts of SSLO in unused bedding and SSLO-IMI (OR = 1.09). These patterns of association were generally consistent across the 4 common bedding materials. In contrast, the association between counts of all bacteria in used bedding and ALL-IMI varied by bedding type, with positive associations observed in quarters exposed to manure solids (OR = 2.29) and organic non-manure (OR = 1.51) and a negative association in quarters exposed to new sand (OR = 0.47). Findings from this study suggest that quarter-level IMI prevalence in late-lactation cows is low in US dairy herds. Furthermore, bedding material type may not be an important risk factor for IMI in late lactation. Higher levels of bacteria in bedding may increase IMI prevalence at dry-off in general, but this relationship is likely to vary according to bedding material type.


Assuntos
Infecções por Klebsiella/veterinária , Mastite Bovina/microbiologia , Leite/microbiologia , Infecções Estafilocócicas/veterinária , Infecções Estreptocócicas/veterinária , Animais , Carga Bacteriana/veterinária , Roupas de Cama, Mesa e Banho/microbiologia , Roupas de Cama, Mesa e Banho/veterinária , Bovinos , Estudos Transversais , Feminino , Klebsiella/isolamento & purificação , Infecções por Klebsiella/epidemiologia , Infecções por Klebsiella/microbiologia , Lactação , Modelos Logísticos , Glândulas Mamárias Animais/microbiologia , Esterco/microbiologia , Mastite Bovina/epidemiologia , Prevalência , Fatores de Risco , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus/isolamento & purificação , Infecções Estreptocócicas/epidemiologia , Infecções Estreptocócicas/microbiologia , Streptococcus/isolamento & purificação
15.
J Dairy Sci ; 102(12): 11401-11413, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31606221

RESUMO

Because cloth udder towels (CUT) may function as a fomite for mastitis-causing pathogens, most udder health laboratories offer towel culture services as a tool to monitor towel hygiene. However, no studies have investigated if an association exists between bacteria levels in CUT and udder health outcomes. The objectives of this cross-sectional study were to (1) describe associations between herd-level measures of towel bacteria count (ToBC) and quarter-level intramammary infection (IMI) status in late-lactation cows, (2) establish pathogen-specific target levels of bacteria in CUT to aid the interpretation of towel culture reports, and (3) identify laundering-related risk factors for high ToBC. The study was conducted in 67 herds from 10 dairy states in the United States that used CUT. These 67 herds were originally recruited as part of a larger (80 herd) cross-sectional study of bedding management. Each herd was visited once during December 2017 to April 2018 and quarter-milk samples (n = 4,656) were collected from late-gestation (>180 d pregnant) cows (n = 1,313). Two recently laundered CUT were collected and a questionnaire was used to collect information about pre-milking teat preparation and CUT management practices. Quarter-level IMI status was determined using standard bacteriologic methods. In addition, colony-forming units of all bacteria (total bacteria), Staphylococcus spp., Streptococcus spp. or Streptococcus-like organisms (SSLO), coliforms, noncoliform gram-negatives, and Bacillus spp. were determined for each pair of CUT (log10 cfu/cm2). The association between ToBC and IMI was determined using multivariable logistic regression with mixed effects. After dichotomizing ToBC into high and low categories, associations between towel management practices and ToBC category were determined using unconditional logistic regression. The quarter-level prevalence of IMI was 19.6%, which was predominantly caused by non-aureus Staphylococcus spp. (NAS; 10.2%) and SSLO (5.1%). The predominant bacteria in CUT were Bacillus spp. (median = 3.13 log10 cfu/cm2). Total bacteria count was not associated with odds of IMI (odds ratio = 1.06), likely due to the predominance of Bacillus spp. in CUT and low number of IMI caused by Bacillus spp. In contrast, counts of Staphylococcus spp. and SSLO were positively associated with odds of IMI caused by NAS (odds ratio = 1.33) and SSLO (odds ratio = 1.45), respectively. Of 12 CUT management practices evaluated, only the failure to use a dryer was identified as a clear predictor of risk for a high ToBC (risk ratio of high coliform count = 8.17). Our study findings suggest that CUT may act as a fomite for NAS and SSLO. We recommend that herds aim to keep counts of Staphylococcus spp. and SSLO in CUT below 32 cfu/cm2 (or 5 cfu/in2), and that laundered towels be completely dried in a hot air dryer.


Assuntos
Infecções por Klebsiella/veterinária , Mastite Bovina/microbiologia , Leite/microbiologia , Infecções Estafilocócicas/veterinária , Infecções Estreptocócicas/veterinária , Animais , Carga Bacteriana/veterinária , Bovinos , Estudos Transversais , Feminino , Klebsiella/isolamento & purificação , Infecções por Klebsiella/epidemiologia , Infecções por Klebsiella/microbiologia , Lactação , Modelos Logísticos , Glândulas Mamárias Animais/microbiologia , Esterco/microbiologia , Mastite Bovina/epidemiologia , Gravidez , Prevalência , Fatores de Risco , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus/isolamento & purificação , Infecções Estreptocócicas/epidemiologia , Infecções Estreptocócicas/microbiologia , Streptococcus/isolamento & purificação
16.
Pesqui. vet. bras ; 39(10): 796-801, Oct. 2019. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1056903

RESUMO

Respiratory diseases are among the most important diseases in sheep flocks. Herein was studied the bacterial etiology of respiratory disease and the clinical signs of 99 female and male sheep breed in the states of São Paulo (SP) and Rio de Janeiro (RJ), Brazil. After physical examination of animals, tracheobronchial flushing samples were obtained. The usual bacteria and Mycoplasma spp. were searched, as well as their association with the clinical status and clinical signs of sheep with respiratory disease. The main observed signs were: tachypnea (75%), increase of rectal temperature (09.4%), mucopurulent/purulent nasal discharge (21.9%), cough (25%), dyspnea (31.2%), changes of lung sounds at auscultation (87.5%) and chest percussion (28.1%) in pneumonic sheep. Non-fermenting gram-negative bacteria and Bacillus sp. were the most isolated bacteria. Microorganisms of the Mollicutes class were molecularly (PCR) detected in 33.3% of the animals. In addition, the specific detection of M. mycoides subsp. capri was described for the first time in sheep from the state of São Paulo, Brazil.(AU)


A doença respiratória é uma das doenças mais importantes em rebanhos ovinos. Esta pesquisa teve como objetivo determinar a etiologia bacteriana da doença respiratória e sua relação com sinais clínicos em ovinos criados nos estados de São Paulo e Rio de Janeiro, Brasil. Noventa e nove ovelhas machos e fêmeas dos Estados de São Paulo (SP) e Rio de Janeiro (RJ) foram estudadas. Após o exame físico, amostras de lavagem traqueobrônquica foram obtidas. A presença de bactérias aeróbias e Mycoplasmaspp. foram estudados, assim como a associação entre os microrganismos e estado clínico e sinais clínicos de doença respiratória em ovinos. As principais manifestações clínicas observadas foram: taquipneia (75%), alta temperatura retal (09,4%), secreção nasal mucopurulenta/purulenta (21,9%), tosse (25%), dispneia (31,2%), sons pulmonares alterados na ausculta (87,5%) e na percussão torácica (28,1%) em ovelhas pneumônicas. Bactérias gram-negativas não fermentadoras e Bacillus sp. foram as bactérias mais isoladas. Microrganismos da classe Mollicutes foram detectados molecularmente (PCR) em 33,3% dos ovinos. Além disso, descreve-se pela primeira vez no estado de São Paulo, Brasil, a detecção do M. mycoides subsp. capri na espécie ovina utilizando a reação de polimerase em cadeia.(AU)


Assuntos
Animais , Infecções por Pasteurella/veterinária , Pneumonia/etiologia , Pneumonia/veterinária , Ovinos , Infecções por Mycoplasma/veterinária , Doenças dos Ovinos/microbiologia , Bacillus/isolamento & purificação , Pasteurella/isolamento & purificação , Klebsiella/isolamento & purificação , Mycoplasma/isolamento & purificação
17.
Rev. esp. quimioter ; 32(5): 426-431, oct. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-188708

RESUMO

INTRODUCCIÓN: La colecistitis constituye una importante causa de ingreso hospitalario. En colecistitis moderada o severa, el retraso en el tratamiento puede acarrear complicaciones graves. Nuestro objetivo es analizar los microorganismos aislados en bilis de pacientes colecistectomizados y su patrón de sensibilidad para evaluar el tratamiento empírico en aquellos casos en que la extirpación quirúrgica de la vesícula deba demorarse. PACIENTES Y MÉTODOS: Estudio descriptivo prospectivo de los cultivos biliares de pacientes sometidos a colecistectomía desde mayo de 2013 hasta febrero de 2015, en el Servicio de Cirugía del Hospital General Universitario de Castellón. RESULTADOS: Se estudiaron 196 pacientes, 83 mujeres (42,3%) y 113 hombres (57,7%), con una media de edad de 61,5 años. Los antibióticos más utilizados como tratamiento empírico fueron piperacilina/tazobactam (77,8%) y amoxicilina/clavulánico (14,8%). En el 46,4% de los pacientes (91/196) los cultivos de bilis fueron positivos. Se aislaron un total de 165 microorganismos. La mayoría eran bacilos gramnegativos (60,5%), principalmente Enterobacterales (91/54,5%), siendo Escherichia coli el microorganismo más frecuente (24%) seguido de Klebsiella spp. (12,5%). Se aislaron 3 E. coli productoras de betalactamasa de espectro extendido (BLEE) y 1 Klebsiella pneumoniae BLEE. No se aislaron microorganismos productores de carbapenemasa ni Staphylococcus aureus resistente a meticilina. CONCLUSIÓN: La microbiota biliar, con predominio de Enterobacterales, es similar a la encontrada en estudios europeos


INTRODUCTION: Cholecystitis is an important cause of hospital admission. In moderate or severe cholecystitis, the delay in treatment can lead to serious complications. Our objective is to analyze the microorganisms isolated in bile from cholecystectomized patients and their sensitivity pattern, to evaluate the empirical treatment in those cases in which the surgical removal of the gallbladder should be delayed. PATIENTS AND METHODS: Prospective descriptive study of biliary cultures of patients undergoing cholecystectomy from May 2013 to February 2015, in the Surgery Department of the Hospital General Universitari de Castelló. RESULTS: We studied 196 patients, 83 women (42.3%) and 113 men (57.7%), with an average age of 61.5 years. The most used antibiotics as empiric treatment were piperacillin/tazobactam (77.8%) and amoxicillin/clavulanic (14.8%). In 46.4% of patients (91/196) bile cultures were positive.165 microorganisms were isolated. The majority were Gram-negative bacilli (60.5%), mainly of the Enterobacterales order (91/54.5%), with Escherichia coli being the most frequent microorganism (24%) followed by Klebsiella spp. (12.5%).3 E. coli with extended-spectrum beta-lactamase (ESBL) and 1 K. pneumoniae with ESBL were isolated. Microorganisms producing carbapenemase and methicillin-resistant Staphylococcus aureus were not isolated. CONCLUSION: The bile microbiota, with a predominance of Enterobacterales is similar to that found in european studies


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Antibacterianos/uso terapêutico , Bile/microbiologia , Colecistectomia , Colecistite/microbiologia , Microbiota , Combinação Amoxicilina e Clavulanato de Potássio/uso terapêutico , Colecistite/cirurgia , Ciprofloxacino/uso terapêutico , Escherichia coli/isolamento & purificação , Bactérias Gram-Negativas/isolamento & purificação , Klebsiella/isolamento & purificação , Metronidazol/uso terapêutico , Testes de Sensibilidade Microbiana , Combinação Piperacilina e Tazobactam/uso terapêutico , Estudos Prospectivos
18.
BMC Res Notes ; 12(1): 607, 2019 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-31547886

RESUMO

OBJECTIVE: Research suggests human norovirus binding to histo-blood group antigen (HBGA)-like molecules on enteric bacteria may enhance viral pathogenesis; however, the properties of these bacterial ligands are not well known. Previous work identified, but did not characterize, seven norovirus-binding bacteria. To further examine this bacteria-virus binding interaction, enteric bacteria were analyzed via Western blot with anti-HBGA antibodies and lectins targeting HBGA-associated sugar components. Virus overlay assays using capsids from six different human norovirus strains further identified responsible ligands and strain dependent binding properties. RESULTS: Each bacterial species possessed varying degrees of HBGA-like activity, and lectin binding further elucidated potential sugar residues involved (N-acetyl-galactosamine, α-D-galactose or α-L-fucose). Both GI and GII norovirus capsids bound specific bacterial ligand sizes, and generally corresponded to anti-HBGA Western blot patterns. A 35-kDa band reacted with all HBGA antibodies, bound all six of the noroviruses tested, and had a high affinity for the lectins. Collectively, this work characterizes the varying carbohydrate residues potentially responsible for norovirus-bacteria interactions and provides a basis for future ligand identification.


Assuntos
Proteínas de Bactérias/metabolismo , Enterobacter cloacae/virologia , Interações Microbianas/genética , Norovirus/genética , Staphylococcus aureus/virologia , Acetilgalactosamina/química , Acetilgalactosamina/metabolismo , Anticorpos Antibacterianos/química , Anticorpos Antibacterianos/metabolismo , Bacillus/isolamento & purificação , Bacillus/virologia , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Antígenos de Grupos Sanguíneos/química , Antígenos de Grupos Sanguíneos/genética , Antígenos de Grupos Sanguíneos/metabolismo , Western Blotting , Capsídeo/química , Capsídeo/metabolismo , Enterobacter cloacae/isolamento & purificação , Fucose/química , Fucose/metabolismo , Galactose/química , Galactose/metabolismo , Microbioma Gastrointestinal/genética , Expressão Gênica , Humanos , Klebsiella/isolamento & purificação , Klebsiella/virologia , Lectinas/química , Lectinas/metabolismo , Ligantes , Mimetismo Molecular , Norovirus/metabolismo , Ligação Proteica , Staphylococcus aureus/isolamento & purificação
19.
Biocontrol Sci ; 24(3): 173-178, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31527349

RESUMO

We investigated the midstream bottom of the Tama River, which flows through Tokyo, to evaluate the occurrence and degree of antibiotic-resistant fecal coliforms including multidrug-resistant fecal coliforms. The genera Klebsiella and Escherichia were the major isolates among the fecal coliforms. For the genus Klebsiella, the highest antibiotic resistance was observed for ampicillin (100%) , followed by kanamycin, tetracycline, cefotaxime, and cefoxitin. The highest resistance to E. coli was found for kanamycin (44.4%) , followed by ampicillin, tetracycline, chloramphenicol, amoxicillin-clavulanate, cefotaxime, ceftazidime, and aztreonam. Multidrug resistance (MDR) was observed in three E. coli isolates. A double disc synergy test confirmed the production of extended-spectrum ß-lactamases by the six-antibiotic-resistant isolate E. coli hfa7, and the strain had CTX-M-1 group gene. Assessments of antibiotic-resistant fecal coliforms at the bottom of the Tama River are important toward the goals of preventing the spread of antibiotic-resistant fecal coliforms in humans, animals, and the environment.


Assuntos
Farmacorresistência Bacteriana , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Sedimentos Geológicos/microbiologia , Klebsiella/efeitos dos fármacos , Klebsiella/isolamento & purificação , Rios/microbiologia , Testes de Sensibilidade Microbiana , Tóquio
20.
Mar Pollut Bull ; 149: 110548, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31550576

RESUMO

The present study is aimed to monitoring the ecological indicator and pathogenic microorganism diversity in pilgrimage places beach sand on the Bay of Bengal coast. The samples were collected from three locations and four different sites, and were analyzed by following standard methods. The results clearly indicates, ritual activities were highly contaminated in the beach sand qualities, and exceeded with the standard permissible limit of WHO, USEPA, EU, CPCB beach sand recreational and other contacts activities including pH (11%), TBC (100%), TCB (97%), FCB (88%), TEB (75%), E. coli (75%), disease-causing possible level of Klebsiella (84%), Shigella (75%), Salmonella (63%) and Vibrio (56%). The statistical tools were applied to find the strong evidence. The current study pointed out the major effects on the diffusion of potentially pathogenic microorganisms along the shoreline provided useful information for the setup of measures for public health protection in the Bay of Bengal coast.


Assuntos
Praias , Baías/microbiologia , Comportamento Ritualístico , Indicadores Ambientais , Monitoramento Biológico/métodos , Enterobacteriaceae/isolamento & purificação , Escherichia coli/isolamento & purificação , Humanos , Índia , Klebsiella/isolamento & purificação , Salmonella/isolamento & purificação , Shigella/isolamento & purificação , Vibrio/isolamento & purificação , Microbiologia da Água
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