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1.
Science ; 368(6486): 78-84, 2020 04 03.
Artigo em Inglês | MEDLINE | ID: mdl-32241946

RESUMO

The design of modular protein logic for regulating protein function at the posttranscriptional level is a challenge for synthetic biology. Here, we describe the design of two-input AND, OR, NAND, NOR, XNOR, and NOT gates built from de novo-designed proteins. These gates regulate the association of arbitrary protein units ranging from split enzymes to transcriptional machinery in vitro, in yeast and in primary human T cells, where they control the expression of the TIM3 gene related to T cell exhaustion. Designed binding interaction cooperativity, confirmed by native mass spectrometry, makes the gates largely insensitive to stoichiometric imbalances in the inputs, and the modularity of the approach enables ready extension to three-input OR, AND, and disjunctive normal form gates. The modularity and cooperativity of the control elements, coupled with the ability to de novo design an essentially unlimited number of protein components, should enable the design of sophisticated posttranslational control logic over a wide range of biological functions.


Assuntos
Receptor Celular 2 do Vírus da Hepatite A/química , Engenharia de Proteínas , Mapas de Interação de Proteínas , Processamento de Proteína Pós-Traducional , Receptor Celular 2 do Vírus da Hepatite A/genética , Humanos , Lógica , Espectrometria de Massas , Biologia Sintética , Linfócitos T/metabolismo , Transcrição Genética , Leveduras/metabolismo
3.
BMC Health Serv Res ; 20(1): 107, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32046710

RESUMO

BACKGROUND: Clinical practice improvements based on quality-register data are influenced by multiple factors. Although there is agreement that information from quality registers is valuable for quality improvement, practical ways of organising register use have been notoriously difficult to realise. The present study sought to investigate the mechanisms that lead various clinicians to use quality registers for improvement. METHODS: This research involves studying individuals' decisions in response to a Swedish programme focusing on increasing the use of quality registers. Through a case study, we focused on heart failure care and its corresponding register: the Swedish Heart Failure Register. The empirical data consisted of a purposive sample collected longitudinally by qualitative methods between 2013 and 2015. In total, 18 semi-structured interviews were carried out. We used realist evaluation to identify contexts, mechanisms, and outcomes. RESULTS: We identified four contexts - registration, use of output data, governance, and improvement projects - that provide conditions for the initiation of specific mechanisms. Given a professional theoretical perspective, we further showed that mechanisms are based on the logics of either organisational improvement or clinical practice. The two logics offer insights into the ways in which clinicians choose to embrace or reject certain registers' initiatives. CONCLUSIONS: We identified a strong path dependence, as registers have historically been tightly linked to the medical profession's competence. Few new initiatives in the studied programme reach the clinical context. We explain this through the lack of an organisational improvement logic and its corresponding mechanisms in the context of the medical profession. Implementation programmes must understand the logic of clinical practice; that is, be integrated with the ways in which work is carried out in everyday practice. Programmes need to be better at helping core health professionals to reach the highest standards of patient care.


Assuntos
Lógica , Melhoria de Qualidade , Sistema de Registros , Pesquisa sobre Serviços de Saúde , Humanos , Suécia
4.
PLoS One ; 15(2): e0228960, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32045454

RESUMO

In this study, we aimed to compare developmental changes in nonsymbolic and symbolic magnitude representations across the elementary school years. For this aim, we used a four-wave longitudinal study with a one-year interval in schoolchildren in grades 1-4 in Russia and Kyrgyzstan (N = 490, mean age was 7.65 years at grade 1). The results of mixed-effects growth models revealed that growth in the precision of symbolic representation was larger than in the nonsymbolic representation. Moreover, growth in nonsymbolic representation was fully explained by growth in fluid intelligence (FI), visuospatial working memory (VSWM) and processing speed (PS). The analysis demonstrated that growth in nonsymbolic magnitude representation was significant only for pupils with a high level of FI and PS, whereas growth in precision of symbolic representation did not significantly vary across pupils with different levels of FI or VSWM.


Assuntos
Desenvolvimento Infantil/fisiologia , Cognição/fisiologia , Inteligência/fisiologia , Adolescente , Criança , Feminino , Humanos , Quirguistão , Lógica , Estudos Longitudinais , Masculino , Conceitos Matemáticos , Memória , Memória de Curto Prazo/fisiologia , Federação Russa , Instituições Acadêmicas , Simbolismo
5.
Oncology ; 98(5): 259-266, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32045926

RESUMO

The optimal type of surgery (e.g., anatomic or non-anatomic resection) or radiofrequency ablation (RFA) for hepatocellular carcinoma (HCC) is still under debate despite numerous comparative studies based on overall survival. This debate continues not only because these endpoints are influenced by non-surgical factors, such as liver function, but because the definition of non-anatomic resection for HCC has remained unclear. The optimal surgery could be logically determined based on the mechanism of local intrahepatic metastasis, that is, the drainage of tumour blood flow (TBF), because HCC spreads locally through tumour blood flowing to the peri-tumourous liver parenchyma. Since TBF is clearly demonstrated by CT scan under hepatic arteriography, the surgical margin can be determined individually based on the drainage of TBF without deteriorating local curability. Controversy regarding RFA and surgery does not result from the curability of treatment itself but from the lack of scientific evidence on safety margins. Based on proper concepts and self-evident truths, an algorithm of loco-regional treatment for HCC is proposed.


Assuntos
Carcinoma Hepatocelular/terapia , Medicina Baseada em Evidências/estatística & dados numéricos , Neoplasias Hepáticas/terapia , Hepatectomia/estatística & dados numéricos , Humanos , Lógica , Tomografia Computadorizada por Raios X/estatística & dados numéricos , Resultado do Tratamento
6.
Nucleic Acids Res ; 48(6): e33, 2020 04 06.
Artigo em Inglês | MEDLINE | ID: mdl-31989175

RESUMO

Light-regulated modules offer unprecedented new ways to control cellular behaviour with precise spatial and temporal resolution. Among a variety of bacterial light-switchable gene expression systems, single-component systems consisting of single transcription factors would be more useful due to the advantages of speed, simplicity, and versatility. In the present study, we developed a single-component light-activated bacterial gene expression system (eLightOn) based on a novel LOV domain from Rhodobacter sphaeroides (RsLOV). The eLightOn system showed significant improvements over the existing single-component bacterial light-activated expression systems, with benefits including a high ON/OFF ratio of >500-fold, a high activation level, fast activation kinetics, and/or good adaptability. Additionally, the induction characteristics, including regulatory windows, activation kinetics and light sensitivities, were highly tunable by altering the expression level of LexRO. We demonstrated the usefulness of the eLightOn system in regulating cell division and swimming by controlling the expression of the FtsZ and CheZ genes, respectively, as well as constructing synthetic Boolean logic gates using light and arabinose as the two inputs. Taken together, our data indicate that the eLightOn system is a robust and highly tunable tool for quantitative and spatiotemporal control of bacterial gene expression.


Assuntos
Regulação Bacteriana da Expressão Gênica/efeitos da radiação , Luz , Rhodobacter sphaeroides/citologia , Rhodobacter sphaeroides/efeitos da radiação , Proteínas de Bactérias/metabolismo , Divisão Celular/efeitos da radiação , Cinética , Lógica , Fatores de Transcrição/metabolismo
7.
Cell Mol Life Sci ; 77(7): 1319-1343, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31612240

RESUMO

Quorum sensing (QS), a microbial cell-to-cell communication process, dynamically regulates a variety of metabolism and physiological activities. In this review, we provide an update on QS applications based on autoinducer molecules including acyl-homoserine lactones (AHLs), auto-inducing peptides (AIPs), autoinducer 2 (AI-2) and indole in population-level control of bacteria, and highlight the potential in developing novel clinical therapies. We summarize the development in the combination of various genetic circuits such as genetic oscillators, toggle switches and logic gates with AHL-based QS devices in Gram-negative bacteria. An overview is then offered to the state-of-the-art of much less researched applications of AIP-based QS devices with Gram-positive bacteria, followed by a review of the applications of AI-2 and indole based QS for interspecies communication among microbial communities. Building on these general-purpose QS applications, we highlight the disruptions and manipulations of QS devices as potential clinical therapies for diseases caused by biofilm formation, antibiotic resistance and the phage invasion. The last part of reviewed literature is dedicated to mathematical modelling for QS applications. Finally, the key challenges and future perspectives of QS applications in monoclonal synthetic biology and synthetic ecology are discussed.


Assuntos
Bactérias/crescimento & desenvolvimento , Percepção de Quorum , Biofilmes/crescimento & desenvolvimento , Microbioma Gastrointestinal , Lógica , Percepção de Quorum/genética , Especificidade da Espécie
8.
Nat Biotechnol ; 38(1): 56-65, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31792407

RESUMO

How transcription factors (TFs) interpret cis-regulatory DNA sequence to control gene expression remains unclear, largely because past studies using native and engineered sequences had insufficient scale. Here, we measure the expression output of >100 million synthetic yeast promoter sequences that are fully random. These sequences yield diverse, reproducible expression levels that can be explained by their chance inclusion of functional TF binding sites. We use machine learning to build interpretable models of transcriptional regulation that predict ~94% of the expression driven from independent test promoters and ~89% of the expression driven from native yeast promoter fragments. These models allow us to characterize each TF's specificity, activity and interactions with chromatin. TF activity depends on binding-site strand, position, DNA helical face and chromatin context. Notably, expression level is influenced by weak regulatory interactions, which confound designed-sequence studies. Our analyses show that massive-throughput assays of fully random DNA can provide the big data necessary to develop complex, predictive models of gene regulation.


Assuntos
Eucariotos/genética , Regulação da Expressão Gênica , Lógica , Regiões Promotoras Genéticas , Sítios de Ligação , DNA/metabolismo , Genes Reporter , Modelos Genéticos , Saccharomyces cerevisiae/genética , Fatores de Transcrição/metabolismo
9.
Talanta ; 208: 120438, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31816755

RESUMO

The threatening of antibiotic drugs for human and environment is being paid more and more attention. Ciprofloxacin (CIP), a strong quinolone antibiotic drug widely used in therapeutic treatments, is the most frequently detected in surface waters among the fluoroquinolones, which represents animal and human health risks. A novel highly fluorescent Ga-based hybrid (Eu3+@1) has been synthesized based on metal-organic framework (MOF) by encapsulating lanthanide cations Eu3+ in its channels. The as-synthesized compound possesses excellent water and pH-independent stability. It displays week red luminescence of Eu3+ in itself and can sense the CIP concentration as turn-on fluorescent probe in the human urine. With addition of CIP, the evident luminescence enhancement is clearly observed from the Eu3+@1. Linear correlation between the fluorescence intensity and the concentration of CIP is investigated, proving the excellent performance of Eu3+@1 in the detection of CIP with linear range (0.01-0.2 mg/mL) and low detection limit (2.4 ppm or 2.4 µg/mL). The response time is also very quick, less than 3 min. Based on these findings, we introduce AND logic gate strategy to the probe. The input of the logic gates (0, 1), (0, 1, 1), (1, 1, 1) cause the different outputs of CIP determination "LOW" (<25 ppm),"NORMAL" (25-76 ppm), "HIGH" (>76 ppm), respectively. The novel strategy can be applied for a real-time CIP concentration evaluation by intelligent discrimination.


Assuntos
Antibacterianos/urina , Ciprofloxacino/urina , Európio/química , Corantes Fluorescentes/química , Gálio/química , Estruturas Metalorgânicas/química , Antibacterianos/química , Ciprofloxacino/química , Humanos , Lógica , Luminescência
11.
PLoS One ; 14(12): e0225299, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31790450

RESUMO

Without election or re-election motivations, what factors have impacted public goods preferences in an authoritarian country such as China? More specifically, what makes political elites be devoted to or not be devoted to local public goods provision? This study, using basic education provision as an example, intends to gauge the impact of leadership selection on public goods provision in China. It is found that career trajectories of politicians have a bearing on basic education provision. The findings suggest that even under a top-down appointment system, homegrown politicians are more willing to cater to local preferences, especially on basic education provision, which suggests an extension of Riker's theory, applied in a non-democratic regime. Numerous studies have examined the impacts of decentralization on a variety of aspects of public governance in different contexts. Nevertheless, the unique contribution of this study is its policy implication that political centralization may not be an effective solution for local public governance even in an authoritarian context.


Assuntos
Grupo com Ancestrais do Continente Asiático/educação , Política Fiscal , Lógica , Política , Autoritarismo , China , Comunismo/economia , Humanos , Liderança , Governo Local , Responsabilidade Social , Fatores Socioeconômicos
12.
Nature ; 576(7787): 446-451, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31801999

RESUMO

Individual neurons in many cortical regions have been found to encode specific, identifiable features of the environment or body that pertain to the function of the region1-3. However, in frontal cortex, which is involved in cognition, neural responses display baffling complexity, carrying seemingly disordered mixtures of sensory, motor and other task-related variables4-13. This complexity has led to the suggestion that representations in individual frontal neurons are randomly mixed and can only be understood at the neural population level14,15. Here we show that neural activity in rat orbitofrontal cortex (OFC) is instead highly structured: single neuron activity co-varies with individual variables in computational models that explain choice behaviour. To characterize neural responses across a large behavioural space, we trained rats on a behavioural task that combines perceptual and value-guided decisions. An unbiased, model-free clustering analysis identified distinct groups of OFC neurons, each with a particular response profile in task-variable space. Applying a simple model of choice behaviour to these categorical response profiles revealed that each profile quantitatively corresponds to a specific decision variable, such as decision confidence. Additionally, we demonstrate that a connectivity-defined cell type, orbitofrontal neurons projecting to the striatum, carries a selective and temporally sustained representation of a single decision variable: integrated value. We propose that neurons in frontal cortex, as in other cortical regions, form a sparse and overcomplete representation of features relevant to the region's function, and that they distribute this information selectively to downstream regions to support behaviour.


Assuntos
Comportamento de Escolha/fisiologia , Neurônios/citologia , Neurônios/fisiologia , Córtex Pré-Frontal/citologia , Animais , Antecipação Psicológica , Aprendizagem por Discriminação , Lógica , Masculino , Modelos Neurológicos , Neostriado/citologia , Neostriado/fisiologia , Vias Neurais , Odorantes/análise , Especificidade de Órgãos , Córtex Pré-Frontal/anatomia & histologia , Córtex Pré-Frontal/fisiologia , Psicometria , Ratos , Ratos Long-Evans , Recompensa
13.
Curr Biol ; 29(24): R1303-R1305, 2019 12 16.
Artigo em Inglês | MEDLINE | ID: mdl-31846675

RESUMO

Quantitative analysis of the giant ciliate Stentor roeselii shows that a single cell can make decisions, based on the ability to switch between several different behaviors in a non-random order.


Assuntos
Aprendizagem da Esquiva , Cilióforos , Cognição , Células Eucarióticas , Lógica
14.
Nat Commun ; 10(1): 5390, 2019 11 26.
Artigo em Inglês | MEDLINE | ID: mdl-31772166

RESUMO

Powerful information processing and ubiquitous computing are crucial for all machines and living organisms. The Watson-Crick base-pairing principle endows DNA with excellent recognition and assembly abilities, which facilitates the design of DNA computers for achieving intelligent systems. However, current DNA computational systems are always constrained by poor integration efficiency, complicated device structures or limited computational functions. Here, we show a DNA arithmetic logic unit (ALU) consisting of elemental DNA logic gates using polymerase-mediated strand displacement. The use of an enzyme resulted in highly efficient logic gates suitable for multiple and cascaded computation. Based on our basic single-rail DNA configuration, additional combined logic gates (e.g., a full adder and a 4:1 multiplexer) have been constructed. Finally, we integrate the gates and assemble the crucial ALU. Our strategy provides a facile strategy for assembling a large-scale complex DNA computer system, highlighting the great potential for programming the molecular behaviors of complicated biosystems.


Assuntos
Computadores Moleculares , DNA de Cadeia Simples/química , DNA de Cadeia Simples/genética , Desenho de Equipamento , Lógica , Reação em Cadeia da Polimerase em Tempo Real
15.
Nat Chem Biol ; 15(12): 1173-1182, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31686032

RESUMO

Efforts to construct synthetic biological circuits with more complex functions have often been hindered by the idiosyncratic behavior, limited dynamic range and crosstalk of commonly utilized parts. Here, we employ de novo RNA design to develop two high-performance translational repressors with sensing and logic capabilities. These synthetic riboregulators, termed toehold repressors and three-way junction (3WJ) repressors, detect transcripts with nearly arbitrary sequences, repress gene expression by up to 300-fold and yield orthogonal sets of up to 15 devices. Automated forward engineering is used to improve toehold repressor dynamic range and SHAPE-Seq is applied to confirm the designed switching mechanism of 3WJ repressors in living cells. We integrate the modular repressors into biological circuits that execute universal NAND and NOR logic and evaluate the four-input expression NOT ((A1 AND A2) OR (B1 AND B2)) in Escherichia coli. These capabilities make toehold and 3WJ repressors valuable new tools for biotechnological applications.


Assuntos
Biossíntese de Proteínas , Biologia Sintética , Escherichia coli/genética , Lógica , Conformação de Ácido Nucleico , RNA/química , RNA/metabolismo
16.
BMC Bioinformatics ; 20(Suppl 9): 390, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31757200

RESUMO

BACKGROUND: Logic Learning Machine (LLM) is an innovative method of supervised analysis capable of constructing models based on simple and intelligible rules. In this investigation the performance of LLM in classifying patients with cancer was evaluated using a set of eight publicly available gene expression databases for cancer diagnosis. LLM accuracy was assessed by summary ROC curve (sROC) analysis and estimated by the area under an sROC curve (sAUC). Its performance was compared in cross validation with that of standard supervised methods, namely: decision tree, artificial neural network, support vector machine (SVM) and k-nearest neighbor classifier. RESULTS: LLM showed an excellent accuracy (sAUC = 0.99, 95%CI: 0.98-1.0) and outperformed any other method except SVM. CONCLUSIONS: LLM is a new powerful tool for the analysis of gene expression data for cancer diagnosis. Simple rules generated by LLM could contribute to a better understanding of cancer biology, potentially addressing therapeutic approaches.


Assuntos
Regulação Neoplásica da Expressão Gênica , Lógica , Aprendizado de Máquina , Neoplasias/diagnóstico , Neoplasias/genética , Adulto , Criança , Bases de Dados Genéticas , Feminino , Humanos , Masculino , Redes Neurais de Computação , Curva ROC
17.
BMC Bioinformatics ; 20(Suppl 9): 344, 2019 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-31757209

RESUMO

BACKGROUND: In silico experiments, with the aid of computer simulation, speed up the process of in vitro or in vivo experiments. Cancer therapy design is often based on signalling pathway. MicroRNAs (miRNA) are small non-coding RNA molecules. In several kinds of diseases, including cancer, hepatitis and cardiovascular diseases, they are often deregulated, acting as oncogenes or tumor suppressors. miRNA therapeutics is based on two main kinds of molecules injection: miRNA mimics, which consists of injection of molecules that mimic the targeted miRNA, and antagomiR, which consists of injection of molecules inhibiting the targeted miRNA. Nowadays, the research is focused on miRNA therapeutics. This paper addresses cancer related signalling pathways to investigate miRNA therapeutics. RESULTS: In order to prove our approach, we present two different case studies: non-small cell lung cancer and melanoma. KEGG signalling pathways are modelled by a digital circuit. A logic value of 1 is linked to the expression of the corresponding gene. A logic value of 0 is linked to the absence (not expressed) gene. All possible relationships provided by a signalling pathway are modelled by logic gates. Mutations, derived according to the literature, are introduced and modelled as well. The modelling approach and analysis are widely discussed within the paper. MiRNA therapeutics is investigated by the digital circuit analysis. The most effective miRNA and combination of miRNAs, in terms of reduction of pathogenic conditions, are obtained. A discussion of obtained results in comparison with literature data is provided. Results are confirmed by existing data. CONCLUSIONS: The proposed study is based on drug discovery and miRNA therapeutics and uses a digital circuit simulation of a cancer pathway. Using this simulation, the most effective combination of drugs and miRNAs for mutated cancer therapy design are obtained and these results were validated by the literature. The proposed modelling and analysis approach can be applied to each human disease, starting from the corresponding signalling pathway.


Assuntos
Lógica , MicroRNAs/genética , Transdução de Sinais/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Simulação por Computador , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Pulmonares/genética , MicroRNAs/metabolismo , Mutação/genética
18.
IET Nanobiotechnol ; 13(7): 665-673, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31573534

RESUMO

Early detection of cancer is very critical because it can reduce the treatment risk and cost. MicroRNAs (miRNAs) have been introduced in recent years as an efficient class of biomarkers for cancer early detection. Now, real-time polymerase chain reaction has been used to profile the miRNA expression, which is costly, time consuming and low accuracy. Most recently, DNA logic gates are used to detect the miRNA expression level that is more accurate and faster than previous methods. The DNA-based logic gates face with serious challenges such as the large complexity and low scalability. In this study, the authors proposed a methodology to design multi-threshold and multi-input DNA-based logic gates in response to specific miRNA inputs in live mammalian cells. The proposed design style can simultaneously recognise multiple miRNAs with different rising and falling thresholds. The design style has been evaluated on the lung cancer biomarkers and the experimental results show the efficiency of the proposed method in terms of accuracy, efficiency and speed.


Assuntos
Biomarcadores Tumorais/genética , Computadores Moleculares , DNA de Neoplasias/análise , Perfilação da Expressão Gênica/métodos , MicroRNAs/genética , Neoplasias/genética , DNA de Neoplasias/genética , Detecção Precoce de Câncer/métodos , Regulação Neoplásica da Expressão Gênica , Humanos , Lógica , Reprodutibilidade dos Testes , Projetos de Pesquisa , Sensibilidade e Especificidade , Transcriptoma
19.
J Biol Phys ; 45(4): 335-366, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31617032

RESUMO

We employ some of the machinery developed in previous work to investigate the inferential and memory functions of quantum-like neural networks. We set up a logical apparatus to implement this in the form of a Gentzen sequent calculus which codifies some of the combinatory rules for the state spaces of the neuronal networks introduced earlier. We discuss memory storage in this context and along the way find formal proof that synchronicity promotes binding and storage. These results lead to an algorithmic fragment in calculus that simulates the memory function known as pattern completion. This claim is tested by noting that the failure of certain steps in the algorithm leads to memory deficits essentially identical to those found in such pathologies as Alzheimer's dementia, schizophrenia, and certain forms of autism. Moreover, a specific "power-of-two" wiring architecture and computational logic, which have been postulated and observed across many brain circuits, emerge spontaneously from our model. We draw conclusions concerning the possible nature of such mental processes qua computations.


Assuntos
Lógica , Memória , Modelos Neurológicos , Teoria Quântica , Rede Nervosa/fisiologia
20.
Psychon Bull Rev ; 26(5): 1596-1618, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31515732

RESUMO

A worrying number of psychological findings are not replicable. Diagnoses of the causes of this "replication crisis," and recommendations to address it, have nearly exclusively focused on methods of data collection, analysis, and reporting. We argue that a further cause of poor replicability is the often weak logical link between theories and their empirical tests. We propose a distinction between discovery-oriented and theory-testing research. In discovery-oriented research, theories do not strongly imply hypotheses by which they can be tested, but rather define a search space for the discovery of effects that would support them. Failures to find these effects do not question the theory. This endeavor necessarily engenders a high risk of Type I errors-that is, publication of findings that will not replicate. Theory-testing research, by contrast, relies on theories that strongly imply hypotheses, such that disconfirmation of the hypothesis provides evidence against the theory. Theory-testing research engenders a smaller risk of Type I errors. A strong link between theories and hypotheses is best achieved by formalizing theories as computational models. We critically revisit recommendations for addressing the "replication crisis," including the proposal to distinguish exploratory from confirmatory research, and the preregistration of hypotheses and analysis plans.


Assuntos
Teoria Psicológica , Psicologia/métodos , Erro Experimental , Simulação por Computador , Coleta de Dados , Humanos , Lógica , Reprodutibilidade dos Testes
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