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1.
Wiad Lek ; 74(5): 1079-1085, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34090268

RESUMO

OBJECTIVE: The aim: The article presents the theoretical substantiation of the value-motivational component of a healthy lifestyle, the diagnostic procedure, the results of quantitative and qualitative analysis of the real state of its formation in students of modern universities; the pedagogical tools of health-oriented, containing interactive and reflexively oriented forms and methods of educating a healthy lifestyle are presented; specific examples of their implementation are given. PATIENTS AND METHODS: Materials and methods: A set of general scientific methods (analysis, synthesis, comparison, systematization, generalization) and diagnostic standardized methods (M. Rokich's "Value orientations" method, R. Berezivska's questionnaire "Attitude to health" (value-motivational scale), questionnaire "Motivational component of a healthy lifestyle" (N. Voloshko, V. Rybalka). RESULTS: Results: A healthy lifestyle is a way of life based on the value attitude to maintaining, improving and strengthening health through motivated activities aimed at improving living conditions, which ensures quality self-realization of the individual in all spheres of life. An essential component of the structure of a healthy lifestyle is the value-motivational component as a set of motives, needs, attitudes to maintain one's own health and fostering a sense of responsibility for it. The pedagogical tools of health orientation are a set of interactive and reflexively oriented forms and methods of forming a healthy lifestyle, which are based on the partner subject-subject interaction of teacher and students, their dialogue, polylogy and cooperation. CONCLUSION: Conclusions:Theoretical understanding and the results of diagnostic research made it possible to determine the nature and structure of a healthy lifestyle, to determine the real state of formation of its value-motivational component and to develop pedagogical tools of health orientation.


Assuntos
Motivação , Universidades , Estilo de Vida Saudável , Humanos , Lógica , Estudantes
2.
BMC Health Serv Res ; 21(1): 580, 2021 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-34134672

RESUMO

BACKGROUND: The French healthcare system is characterised by a shift towards outpatient care and the desire to develop telemedicine affirmed in the collective commitment "Ma santé 2022" presented by President Macron in 2018. In France, remote patient follow up has recently been developed in the active phase of cancer treatment inspired by the patient navigation approach used in other countries. According to Service-Dominant Logic (S-D L), patients become more active. Their role in co-production of services is strengthened and their behaviours changed. Telephone follow-ups can contribute to modifying the relationship between the patient and the nurse navigators in charge of it, moving logically from a passive attitude from the patient to a more active one. METHODS: This study was carried out at Léon Bérard, a cancer control unit, in France. It concerned patients treated in an oncohaematology department, who benefited from telephone follow-ups carried out by nurse specialists during the active phase of their treatment. The multidisciplinary research team including social science researchers, physicians and carers developed a research protocol to study this pilot case. Essentially based on a qualitative approach, it was validated by the centre's management to study this follow-up on patients' behaviours. The 1st phase of the research, based on 24 semi-structured interviews with patients undergoing treatment undertaken from November 2018 to September 2019, is presented. RESULTS: The Telephone follow-up was a positive experience for all patients. The action of the nurse specialist helped to develop certain dimensions of in-role and extra-role behaviour that created value. The patients' discourse has reported a positive follow-up in its clinical dimensions, its psychological dimensions and an enhanced quality of life. We detected a patient activation through their roles but it remained limited. The telephone follow-up also created a patient dependency. CONCLUSIONS: The telephone follow-up is a relevant tool for patients undergoing treatment and it deserves to be more widely deployed. It brings comfort and creates a relationship based on trust but at the same time it limits the emancipation of the patient, which is a central element of the S-D logic and its empowerment.


Assuntos
Neoplasias , Enfermeiras Especialistas , Seguimentos , França , Hospitais , Humanos , Lógica , Neoplasias/terapia , Qualidade de Vida , Telefone
3.
Nanoscale ; 13(17): 7988-8003, 2021 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-33885099

RESUMO

Nucleic acid-based nanodevices have been widely used in the fields of biosensing and nanomedicine. Traditionally, the majority of these nanodevices were first constructed in vitro using synthetic DNA or RNA oligonucleotides and then delivered into cells. Nowadays, the emergence of genetically encoded RNA nanodevices has provided a promising alternative approach for intracellular analysis and regulation. These genetically encoded RNA-based nanodevices can be directly transcribed and continuously produced inside living cells. A variety of highly precise and programmable nanodevices have been constructed in this way during the last decade. In this review, we will summarize the recent advances in the design and function of these artificial genetically encoded RNA nanodevices. In particular, we will focus on their applications in regulating cellular gene expression, imaging, logic operation, structural biology, and optogenetics. We believe these versatile RNA-based nanodevices will be broadly used in the near future to probe and program cells and other biological systems.


Assuntos
Ácidos Nucleicos , RNA , Lógica , Nanomedicina , Oligonucleotídeos
5.
BMJ Open ; 11(3): e044649, 2021 03 26.
Artigo em Inglês | MEDLINE | ID: mdl-33771827

RESUMO

OBJECTIVE: This study used a programme logic model to describe the inputs, activities and outputs of the '10,000 Lives' smoking cessation initiative in Central Queensland, Australia. DESIGN: A programme logic model provided the framework for the process evaluation of '10,000 Lives'. The data were collected through document review, observation and key informant interviews and subsequently analysed after coding and recoding into classified themes, inputs, activities and outputs. SETTING: The prevalence of smoking is higher in the Central Queensland region of Australia compared with the national and state averages. In 2017, Central Queensland Hospital and Health Services set a target to reduce the percentage of adults who smoke from 16.7% to 9.5% in the Central Queensland region by 2030 as part of their strategic vision ('Destination 2030'). Achieving this target is equivalent to 20,000 fewer smokers in Central Queensland, which should result in 10,000 fewer premature deaths due to smoking-related diseases. To translate this strategic goal into an actionable smoking cessation initiative, the '10,000 Lives' health promotion programme was officially launched on 1 November 2017. RESULT: The activities of the initiative coordinated by a senior project officer included building clinical and community taskforces, organising summits and workshops, and regular communications to stakeholders. Public communication strategies (e.g., Facebook, radio, community exhibitions of '10,000 Lives' and health-related events) were used to promote available smoking cessation support to the Central Queensland community. CONCLUSION: The '10,000 Lives' initiative provides an example of a coordinated health promotion programme to increase smoking cessation in a regional area through harnessing existing resources and strategic partnerships (e.g., Quitline). Documenting and describing the process evaluation of the '10,000 Lives' model is important so that it can be replicated in other regional areas with high prevalence of smoking.


Assuntos
Abandono do Hábito de Fumar , Adulto , Austrália , Promoção da Saúde , Humanos , Lógica , Queensland
6.
Nanoscale ; 13(10): 5429-5434, 2021 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-33682870

RESUMO

In vitro transcription is a convenient platform for fabricating nanodevices and has been used for assembling synthetic networks. However, it remains challenging to regulate synthetic cell-free in vitro transcription by multiple stimuli in a simple and programmable way. We proposed a strategy to regulate in vitro transcription by controlling the transcription templates' promoter domain via variable DNA inputs. To demonstrate the utility of this strategy, various logic circuits and cascading circuits were implemented. With the advantage of simplicity, modularity, programmability, and extensibility, the proposed strategy has potential in biocomputing, bioanalytical, and therapeutic applications.


Assuntos
DNA , Lógica , Regiões Promotoras Genéticas
7.
Nanoscale ; 13(11): 5706-5713, 2021 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-33683263

RESUMO

Living organisms use interconnected chemical reaction networks (CRNs) to exchange information with the surrounding environment and respond to diverse external stimuli. Inspired by nature, numerous artificial CRNs with a complex information processing function have been recently introduced, with DNA as one of the most attractive engineering materials. Although much progress has been made in DNA-based CRNs in terms of controllable reaction dynamics and molecular computation, the effective integration of signal translation with information processing in a single CRN remains to be difficult. In this work, we introduced a stimuli-responsive DNA reaction network capable of integrated information translation and processing in a stepwise manner. This network is designed to integrate sensing, translation, and decision-making operations by independent modules, in which various logic units capable of performing different functions were realized, including information identification (YES and OR gates), integration (AND and AND-AND gates), integration-filtration (AND-AND-NOT gate), comparison (Comparator), and map-to-map analysis (Feynman gate). Benefitting from the modular and programmable design, continuous and parallel processing operations are also possible. With the innovative functions, we show that the DNA network is a highly useful addition to the current DNA-based CRNs by offering a bottom-up strategy to design devices capable of cascaded information processing with high efficiency.


Assuntos
Computadores Moleculares , DNA , Lógica
10.
Anal Chim Acta ; 1149: 338213, 2021 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-33551052

RESUMO

Here, a MNAzyme probes mediated DNA logic platform was developed for microRNAs (miRNAs) logic detection and cancer cells identification. A series of MNAzyme probes containing the cleavage active center were designed. Four types of logic gates were constructed, including YES, AND, XOR and NOR gate. These logic gates used miRNAs that were high expression in cancer cells as logic inputs and used MNAzyme cleavage amplification reaction to output signals. For the construction of intracellular logic gates, MnO2 nanosheets were used as carriers and cofactor providers. When MnO2 nanoprobes entered the cells through endocytosis, the intracellular glutathione degraded the MnO2 nanosheets to release the cofactor Mn2+ and MNAzyme probes. The MNAzyme probes bound to the miRNAs and catalyze the MNAzyme cleavage amplification reaction, producing enhanced fluorescent signal with "true" output. The logic detection of miRNAs was achieved by integrating information from the AND, XOR and NOR logic gates. Moreover, through the construction of intracellular YES and AND logic gates, the cancer cells identification, especially the identification of same type of cancer cells with different phenotypes was achieved. These experimental results showed that this platform held great promise in accurate diagnosis and treatment of cancer.


Assuntos
MicroRNAs , Neoplasias , DNA , Lógica , Compostos de Manganês , MicroRNAs/genética , Neoplasias/diagnóstico , Neoplasias/genética , Óxidos
11.
Science ; 371(6529): 576, 2021 02 05.
Artigo em Inglês | MEDLINE | ID: mdl-33542128
12.
Sensors (Basel) ; 21(3)2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-33573308

RESUMO

Wireless sensor networks (WSN) are widely used to provide users with convenient services such as health-care, and smart home. To provide convenient services, sensor nodes in WSN environments collect and send the sensing data to the gateway. However, it can suffer from serious security issues because susceptible messages are exchanged through an insecure channel. Therefore, secure authentication protocols are necessary to prevent security flaws in WSN. In 2020, Moghadam et al. suggested an efficient authentication and key agreement scheme in WSN. Unfortunately, we discover that Moghadam et al.'s scheme cannot prevent insider and session-specific random number leakage attacks. We also prove that Moghadam et al.'s scheme does not ensure perfect forward secrecy. To prevent security vulnerabilities of Moghadam et al.'s scheme, we propose a secure and lightweight mutual authentication protocol for WSNs (WSN-SLAP). WSN-SLAP has the resistance from various security drawbacks, and provides perfect forward secrecy and mutual authentication. We prove the security of WSN-SLAP by using Burrows-Abadi-Needham (BAN) logic, Real-or-Random (ROR) model, and Automated Verification of Internet Security Protocols and Applications (AVISPA) simulation. In addition, we evaluate the performance of WSN-SLAP compared with existing related protocols. We demonstrate that WSN-SLAP is more secure and suitable than previous protocols for WSN environments.


Assuntos
Segurança Computacional , Confidencialidade , Simulação por Computador , Lógica
13.
J Neurosci ; 41(5): 813-822, 2021 02 03.
Artigo em Inglês | MEDLINE | ID: mdl-33431633

RESUMO

The sensory and cognitive abilities of the mammalian neocortex are underpinned by intricate columnar and laminar circuits formed from an array of diverse neuronal populations. One approach to determining how interactions between these circuit components give rise to complex behavior is to investigate the rules by which cortical circuits are formed and acquire functionality during development. This review summarizes recent research on the development of the neocortex, from genetic determination in neural stem cells through to the dynamic role that specific neuronal populations play in the earliest circuits of neocortex, and how they contribute to emergent function and cognition. While many of these endeavors take advantage of model systems, consideration will also be given to advances in our understanding of activity in nascent human circuits. Such cross-species perspective is imperative when investigating the mechanisms underlying the dysfunction of early neocortical circuits in neurodevelopmental disorders, so that one can identify targets amenable to therapeutic intervention.


Assuntos
Neocórtex/citologia , Neocórtex/crescimento & desenvolvimento , Rede Nervosa/citologia , Rede Nervosa/crescimento & desenvolvimento , Células-Tronco Neurais/fisiologia , Neurônios/fisiologia , Animais , Humanos , Lógica
14.
Cogn Process ; 22(2): 353-362, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33404900

RESUMO

How can we rethink 'rationality' in the wake of animal and artificial intelligence studies? Can nonhuman systems be rational in any nontrivial sense? In this paper, we propose that all organisms, under certain circumstances, exhibit rationality to a diverse degree and aspect in the sense of the standard picture (SP): Their inferential processes conform to logic and probability rules. We first show that according to Calvo and Friston (J R Soc Interface 14(131):20170096, 2017) and Orlandi (2018), all biological systems must embody a top-down process (active inference) to minimize free energy. Next, based on Maddy's (Second philosophy, Oxford University Press, Oxford, 2007; The logical must: Wittgenstein on logic, Oxford University Press, Oxford, 2014) analysis, we argue that this inferential process conforms to logic and probability rules; thus, it satisfies the SP, which explains the rudimentary logic and arithmetic (e.g., categorizing and numbering) found among pigeons and mice. We also hold that the mammalian brain is only one among many ways of implementing rationality. Finally, we discuss data from microorganisms to support this view.


Assuntos
Inteligência Artificial , Lógica , Animais , Encéfalo , Humanos , Camundongos , Filosofia , Probabilidade
15.
Nanoscale ; 13(4): 2100-2112, 2021 Feb 04.
Artigo em Inglês | MEDLINE | ID: mdl-33475669

RESUMO

SemiSynBio is an emerging topic toward the construction of platforms for next-generation information processing. Recent research has indicated its promising prospect toward information processing including algorithm design and pattern manipulation with the DNA TMSD reaction, which is one of the cores of the SemiSynBio technology route. The DNA TMSD reaction is the process in which an invader strand displaces the incumbent strand from the gate strand through initiation at the exposed toehold domain. Also, the DNA TMSD reaction generally involves three processes: toehold association, branch migration and strand disassociation. Herein, we review the recent progress on information processing with the DNA TMSD reaction. We highlight the diverse developments on information processing with the logic circuit, analog circuit, combinational circuit and information relay with the DNA origami structure. Additionally, we explore the current challenges and various trends toward the design and application of the DNA TMSD reaction in future information processing.


Assuntos
DNA , Lógica
16.
Chem Commun (Camb) ; 57(9): 1125-1128, 2021 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33410447

RESUMO

Two fragments of the COVID-19 genome (specific and homologous) were used as two inputs to construct an AND logic gate for COVID-19 detection based on exonuclease III and DNAzyme. The detection sensitivity of the assay can reach fM levels. Satisfactory recovery values were obtained in real sample analysis.


Assuntos
Teste para COVID-19 , COVID-19/diagnóstico , DNA Viral/análise , SARS-CoV-2/genética , Saliva/virologia , DNA Catalítico , DNA Viral/sangue , DNA Viral/urina , Exodesoxirribonucleases , Genoma Viral , Humanos , Lógica
17.
Nat Commun ; 12(1): 567, 2021 01 25.
Artigo em Inglês | MEDLINE | ID: mdl-33495464

RESUMO

The regulatory elements controlling gene expression during acute inflammation are not fully elucidated. Here we report the identification of a set of NF-κB-bound elements and common chromatin landscapes underlying the acute inflammatory response across cell-types and mammalian species. Using primary vascular endothelial cells (human/mouse/bovine) treated with the pro-inflammatory cytokine, Tumor Necrosis Factor-α, we identify extensive (~30%) conserved orthologous binding of NF-κB to accessible, as well as nucleosome-occluded chromatin. Regions with the highest NF-κB occupancy pre-stimulation show dramatic increases in NF-κB binding and chromatin accessibility post-stimulation. These 'pre-bound' regions are typically conserved (~56%), contain multiple NF-κB motifs, are utilized by diverse cell types, and overlap rare non-coding mutations and common genetic variation associated with both inflammatory and cardiovascular phenotypes. Genetic ablation of conserved, 'pre-bound' NF-κB regions within the super-enhancer associated with the chemokine-encoding CCL2 gene and elsewhere supports the functional relevance of these elements.


Assuntos
Cromatina/genética , Células Endoteliais/metabolismo , Regulação da Expressão Gênica/genética , Inflamação/genética , NF-kappa B/metabolismo , Sequências Reguladoras de Ácido Nucleico/genética , Doença Aguda , Animais , Sítios de Ligação/genética , Bovinos , Células Cultivadas , Quimiocina CCL2/genética , Quimiocina CCL2/metabolismo , Cromatina/metabolismo , Sequência Conservada/genética , Células Endoteliais/citologia , Células Endoteliais/efeitos dos fármacos , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Inflamação/metabolismo , Inflamação/patologia , Lógica , Camundongos , Modelos Genéticos , Ligação Proteica , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/farmacologia
18.
Nat Commun ; 12(1): 325, 2021 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-33436562

RESUMO

A crucial step towards engineering biological systems is the ability to precisely tune the genetic response to environmental stimuli. In the case of Escherichia coli inducible promoters, our incomplete understanding of the relationship between sequence composition and gene expression hinders our ability to predictably control transcriptional responses. Here, we profile the expression dynamics of 8269 rationally designed, IPTG-inducible promoters that collectively explore the individual and combinatorial effects of RNA polymerase and LacI repressor binding site strengths. We then fit a statistical mechanics model to measured expression that accurately models gene expression and reveals properties of theoretically optimal inducible promoters. Furthermore, we characterize three alternative promoter architectures and show that repositioning binding sites within promoters influences the types of combinatorial effects observed between promoter elements. In total, this approach enables us to deconstruct relationships between inducible promoter elements and discover practical insights for engineering inducible promoters with desirable characteristics.


Assuntos
Isopropiltiogalactosídeo/farmacologia , Lógica , Regiões Promotoras Genéticas , Sítios de Ligação , Fenômenos Biofísicos , RNA Polimerases Dirigidas por DNA/metabolismo , Escherichia coli/efeitos dos fármacos , Escherichia coli/metabolismo , Fluorescência , Genes Reporter , Mutação/genética , Regiões Operadoras Genéticas/genética , Ligação Proteica , Reprodutibilidade dos Testes , Termodinâmica , Fatores de Transcrição/metabolismo
19.
Nat Commun ; 12(1): 355, 2021 01 13.
Artigo em Inglês | MEDLINE | ID: mdl-33441561

RESUMO

The implementation of Boolean logic circuits in cells have become a very active field within synthetic biology. Although these are mostly focussed on the genetic components alone, the context in which the circuit performs is crucial for its outcome. We characterise 20 genetic NOT logic gates in up to 7 bacterial-based contexts each, to generate 135 different functions. The contexts we focus on are combinations of four plasmid backbones and three hosts, two Escherichia coli and one Pseudomonas putida strains. Each gate shows seven different dynamic behaviours, depending on the context. That is, gates can be fine-tuned by changing only contextual parameters, thus improving the compatibility between gates. Finally, we analyse portability by measuring, scoring, and comparing gate performance across contexts. Rather than being a limitation, we argue that the effect of the genetic background on synthetic constructs expands functionality, and advocate for considering context as a fundamental design parameter.


Assuntos
Algoritmos , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Modelos Genéticos , Pseudomonas putida/genética , Escherichia coli/citologia , Redes Reguladoras de Genes , Lógica , Pseudomonas putida/citologia , Especificidade da Espécie , Biologia Sintética/métodos
20.
Food Chem ; 348: 129098, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-33515942

RESUMO

We report the modular design and synthesis of an amine dangled Schiff base quinoline-morpholine conjugate (QMC) for highly selective detection of Pb2+ ions via fluorimetry. The sensing strategy of QMC towards Pb2+ ion exhibits a large blue shift with fluorescent enhancement via the intramolecular charge transfer (ICT) process. At the same time, QMC coordination with Pb2+, the CN single bond rotation between quinoline and morpholine rings and the CN isomerization process were blocked. Best of our knowledge, this is the first blue shifted turn-on fluorescent chemosensor for Pb2+ ion via the ICT process. Furthermore, QMC selectively detects Pb2+ ion without any interference with alkali, alkaline earth, and transition metal ions, and limit of detection (LOD) downs to 13 µM, which is a permissible level of Pb2+ ion in drinking water reported by WHO. The 1:2 binding stoichiometry between QMC and Pb2+ was confirmed by fluorimetric, 1H NMR titration, mass spectrometry, and theoretical studies. Finally, QMC was potentially applied for the sensing of Pb2+ ions in milk, red wine, live cells and an INHIBIT molecular logic function was constructed by using Pb2+ and EDTA as chemical inputs.


Assuntos
Corantes Fluorescentes/química , Lógica , Leite/química , Imagem Molecular/métodos , Quinolinas/química , Animais , Chumbo/análise , Chumbo/química , Limite de Detecção , Bases de Schiff/química , Espectrometria de Fluorescência
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