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1.
Cell Host Microbe ; 27(3): 358-375.e7, 2020 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-32101704

RESUMO

Despite the recognized capacity of the gut microbiota to regulate intestinal lipid metabolism, the role of specific commensal species remains undefined. Here, we aimed to understand the bacterial effectors and molecular mechanisms by which Lactobacillus paracasei and Escherichia coli regulate lipid metabolism in enterocytes. We show that L-lactate produced by L. paracasei inhibits chylomicron secretion from enterocytes and promotes lipid storage by a mechanism involving L-lactate absorption by enterocytes, its conversion to malonyl-CoA, and the subsequent inhibition of lipid beta-oxidation. In contrast, acetate produced by E. coli also inhibits chylomicron secretion by enterocytes but promotes lipid oxidation by a mechanism involving acetate absorption by enterocytes, its metabolism to acetyl-CoA and AMP, and the subsequent upregulation of the AMPK/PGC-1α/PPARα pathway. Our study opens perspectives for developing specific bacteria- and metabolite-based therapeutic interventions against obesity, atherosclerosis, and malnutrition by targeting lipid metabolism in enterocytes.


Assuntos
Enterócitos/metabolismo , Escherichia coli/metabolismo , Fermentação , Lactobacillus paracasei/metabolismo , Metabolismo dos Lipídeos , Simbiose , Animais , Linhagem Celular , Quilomícrons , Enterócitos/microbiologia , Feminino , Intestinos/microbiologia , Camundongos Endogâmicos C57BL
2.
J Med Microbiol ; 68(9): 1359-1366, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31364964

RESUMO

Methodology. Biochemical and molecular methods were used to identify 100 lactobacilli isolated from rectal swabs. Among these, L. paracasei ssp. paracasei LP5 and L. brevis LP9 showed significant antibacterial activity against S. agalactiae and L. monocytogenes. Accordingly, characterization of their bacteriocins, BacLP5 and BacLP9, was conducted to obtain information on their kinetic production, sensitivity to chemico-physical parameters and molecular weight. To investigate the possible use of the two Lactobacillus strains as probiotics, their gastrointestinal resistance, cellular adhesiveness and sensitivity to antibiotics were also studied.Results. The obtained data show that BacLP5 and BacLP9 most likely belong to class II bacteriocins and both have a molecular weight of approximately 3 kDa. The production of BacLP5 and BacLP9 started after 4 h (40 and 80 AU ml-1), respectively. Both of the Lactobacillus strains survived gastric and intestinal juices well and showed adhesive capability on HEp-2 cells.Conclusion. Due to their peculiar antimicrobial characteristics, L. paracasei ssp. paracasei LP5 and L. brevis LP9 are suitable for use in the treatment of vaginal disorders, through both oral and transvaginal administration.


Assuntos
Antibacterianos/metabolismo , Bacteriocinas/metabolismo , Lactobacillus brevis/metabolismo , Lactobacillus paracasei/metabolismo , Antibacterianos/química , Antibacterianos/isolamento & purificação , Aderência Bacteriana , Bacteriocinas/química , Bacteriocinas/isolamento & purificação , Ácidos e Sais Biliares/farmacologia , Linhagem Celular , Fenômenos Químicos , Suco Gástrico/metabolismo , Humanos , Lactobacillus brevis/classificação , Lactobacillus brevis/isolamento & purificação , Lactobacillus paracasei/classificação , Lactobacillus paracasei/isolamento & purificação , Listeria monocytogenes/efeitos dos fármacos , Listeria monocytogenes/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Viabilidade Microbiana/efeitos dos fármacos , Peso Molecular , Probióticos , Reto/microbiologia , Streptococcus agalactiae/efeitos dos fármacos , Streptococcus agalactiae/crescimento & desenvolvimento
3.
Chirality ; 31(10): 892-897, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31423658

RESUMO

Optically active aromatic alcohols are valuable chiral building blocks of many natural products and chiral drugs. Lactobacillus paracasei BD87E6, which was isolated from a cereal-based fermented beverage, was shown as a biocatalyst for the bioreduction of 1-(benzofuran-2-yl) ethanone to (S)-1-(benzofuran-2-yl) ethanol with highly stereoselectivity. The bioreduction conditions were optimized using L. paracasei BD87E6 to obtain high enantiomeric excess (ee) and conversion. After optimization of the bioreduction conditions, it was shown that the bioreduction of 1-(benzofuran-2-yl)ethanone was performed in mild reaction conditions. The asymmetric bioreduction of the 1-(benzofuran-2-yl)ethanone had reached 92% yield with ee of higher than 99.9% at 6.73 g of substrate. Our study gave the first example for enantiopure production of (S)-1-(benzofuran-2-yl)ethanol by a biological green method. This process is also scalable and has potential in application. In this study, a basic and novel whole-cell mediated biocatalytic method was performed for the enantiopure production of (S)-1-(benzofuran-2-yl)ethanol in the aqueous medium, which empowered the synthesis of a precious chiral intermediary process to be converted into a sophisticated molecule for drug production.


Assuntos
Benzofuranos/síntese química , Etanol/análogos & derivados , Etanol/síntese química , Química Verde/métodos , Lactobacillus paracasei/metabolismo , Biocatálise , Concentração de Íons de Hidrogênio , Lactobacillus paracasei/isolamento & purificação , Estereoisomerismo , Temperatura Ambiente
4.
Molecules ; 24(16)2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31426284

RESUMO

Allergic disease is one of the most important and common health problems worldwide. We have previously demonstrated that a fig leaf-derived lactic acid bacterium Lactobacillus (Lb.) paracasei IJH-SONE68 produces a novel exopolysaccharide (EPS). Furthermore, we have shown that the EPS inhibits the catalytic activity of hyaluronidase (EC 3.2.1.36) promoting inflammatory reactions. To evaluate the anti-allergy and anti-inflammatory effects of the EPS, in the present study, we employed the picryl-chloride-induced delayed-type (type IV) allergy model mice, which is used to evaluate the contact dermatitis. Oral administration of the EPS was observed to reduce the ear swelling in the model mice. We also observed that the overexpression of ear interleukin-4 (T helper (Th) 2 cytokine) mRNA and the increase in serum immunoglobulin E (IgE) are repressed. However, the expression of interferon-γ (Th1 cytokine) was not accelerated in all of the allergen-challenged model mice. The improvement may be responsible for the Th2 downregulation rather than the Th1 upregulation. In addition, the symptom of immediate-type (type I) allergy model mice was improved by oral administration of the IJH-SONE68 cell (data not shown). We can conclude that the IJH-SONE68-derived EPS is useful to improve the type I and IV allergies including atopic dermatitis.


Assuntos
Antialérgicos/farmacologia , Anti-Inflamatórios/farmacologia , Dermatite de Contato/prevenção & controle , Fármacos Dermatológicos/farmacologia , Lactobacillus paracasei/química , Polissacarídeos Bacterianos/farmacologia , Administração Oral , Alérgenos/imunologia , Alérgenos/metabolismo , Animais , Antialérgicos/isolamento & purificação , Anti-Inflamatórios/isolamento & purificação , Dermatite de Contato/etiologia , Dermatite de Contato/imunologia , Dermatite de Contato/patologia , Fármacos Dermatológicos/isolamento & purificação , Modelos Animais de Doenças , Orelha , Expressão Gênica/efeitos dos fármacos , Hialuronoglucosaminidase/antagonistas & inibidores , Hialuronoglucosaminidase/imunologia , Hialuronoglucosaminidase/metabolismo , Imunoglobulina E/sangue , Imunoglobulina E/genética , Interferon gama/genética , Interferon gama/imunologia , Interleucina-4/genética , Interleucina-4/imunologia , Lactobacillus paracasei/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Cloreto de Picrila/administração & dosagem , Polissacarídeos Bacterianos/isolamento & purificação , Células Th1/efeitos dos fármacos , Células Th1/imunologia , Células Th2/efeitos dos fármacos , Células Th2/imunologia
5.
PLoS One ; 14(7): e0219558, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31295295

RESUMO

The aim of this research was to improve nutritive value of fishmeal-based feed by lactobacilli in order to achieve satisfactory nutrient availability needed to support fish development. Feed was solid-state treated at a laboratory scale with the combination of Lactobacillus paracasei subsp. paracasei BGHN14 and Lactobacillus rhamnosus BGT10 in different experimental settings, which included the variation of strain ratio, total lactobacilli concentration, percentage of moisture and duration of incubation. Short peptides, soluble proteins, phospho-, neutral and unsaturated lipids were quantified. Differences among treated and control feeds were evaluated by Student t-test, while Gaussian process regression (GPR) modeling was employed to simulate the incubation process and define the optimal treatment combination in the context of overall feed nutritional profile. Treatment duration was shown to be the critical determinant of final outcome, either as single factor or via interaction with strain ratio. Optimal nutrient balance was achieved with 12 h incubation period, 260% moisture, 75:25 and 50:50 BGHN14:BGT10 ratios and 200 mg of lactobacilli per g of dry feed. This study should serve as the basis for large-scale tests which would simulate on-farm production of both fishmeal-based and unconventional, lower cost aquafeed with added value.


Assuntos
Ração Animal , Peixes/fisiologia , Lactobacillus paracasei/metabolismo , Lactobacillus rhamnosus/metabolismo , Animais , Disponibilidade Biológica , Valor Nutritivo , Probióticos/metabolismo , Alimentos Marinhos/microbiologia
6.
Lett Appl Microbiol ; 69(3): 148-154, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31278768

RESUMO

Consumption of probiotics has an important role in colorectal cancer prevention. In this study, we aimed to explore that the cell wall protein fractions from Lactobacillus paracasei could induce apoptosis on Caco-2 cell line. The cell wall proteins from L. paracasei were fractionated by gel filtration chromatography (F1, F2 and F3) and characterized by polyacrylamide gel electrophoresis (SDS-PAGE). The anticancer properties were evaluated using MTT assay and Annexin V-FITC/PI staining. Administration of L. paracasei increased a significant concentration- and time-dependent anti-proliferative effect on Caco-2 cell line, determined by cell viability assays. However, a dramatic decrease in cell viability of Caco-2 cells was observed at the concentration of 100 µg ml-1 of F1 L. paracasei for 72 h (58% cell viability, P < 0·05) The results showed that F1 L. paracasei could induce apoptosis in Caco-2 cancer cell line by increased in annexin V and propidium iodide staining for 72 h (up to 90·6%, P < 0·001). These results indicated the importance of the anticancer effects of cell wall protein fractions of L. paracasei in human colon carcinoma Caco-2 cell line. Thus, cell wall protein fractions of L. paracasei can be a potential chemotherapeutic agent against Caco-2 cell lines. SIGNIFICANCE AND IMPACT OF THE STUDY: Significance and Impact of the Study: Our findings revealed that the newly identified cell wall protein fractions from probiotic Lactobacillus paracasei inhibit the cell growth of human colon carcinoma cell line (Caco-2), and the results indicated that the cell wall proteins from L. paracasei can be a potential chemotherapeutic agent against Caco-2 cell lines.


Assuntos
Antineoplásicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Parede Celular/metabolismo , Neoplasias do Colo/tratamento farmacológico , Lactobacillus paracasei/metabolismo , Proteínas de Membrana/farmacologia , Apoptose/efeitos dos fármacos , Células CACO-2 , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Humanos , Intestinos/citologia , Probióticos/farmacologia
7.
Appl Microbiol Biotechnol ; 103(18): 7617-7634, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31359102

RESUMO

Various Lactobacillus paracasei strains are found in diverse environments, including dairy and plant materials and the intestinal tract of humans and animals, and are also used in the food industry or as probiotics. In this study, we have isolated a new strain L. paracasei subsp. paracasei IBB3423 from samples of raw cow milk collected in a citizen science project. IBB3423 showed some desired probiotic features such as high adhesion capacity and ability to metabolize inulin. Its complete genome sequence comprising the chromosome of 3,183,386 bp and two plasmids of 5986 bp and 51,211 bp was determined. In silico analysis revealed numerous genes encoding proteins involved in carbohydrate metabolism and of extracellular localization likely supporting interaction with host tissues. In vitro tests confirmed the high adhesion capacity of IBB3423 and showed that it even exceeds that of the highly adhesive L. rhamnosus GG. Curing of the larger plasmid indicated that the adhesive properties depend on the plasmid and thus could be determined by its pilus-encoding spaCBA genes.


Assuntos
Aderência Bacteriana , Genoma Bacteriano , Genômica , Lactobacillus paracasei/genética , Lactobacillus paracasei/metabolismo , Leite/microbiologia , Animais , Metabolismo dos Carboidratos , Fímbrias Bacterianas/genética , Inulina/metabolismo , Lactobacillus paracasei/isolamento & purificação , Família Multigênica , Plasmídeos , Probióticos/isolamento & purificação
8.
J Sci Food Agric ; 99(12): 5293-5302, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31006866

RESUMO

BACKGROUND: Growing challenges of resource depletion, food security and environmental protection are putting stress on the development of biorefinery processes for bioprocessing of residues from food and agro-industry into value-added products. In this study, the simultaneous production of lactic acid (LA) and livestock feed on a combined substrate based on molasses and potato stillage by Lactobacillus paracasei NRRL B-4564 immobilized onto sunflower seed hull (SSH), brewer's spent grain (BSG) and sugar beet pulp (SBP) was studied. RESULTS: The highest total LA concentration of 399 g L-1 with overall productivity of 1.27 g L-1  h-1 was achieved in repeated batch fermentation by SBP-immobilized biocatalyst, followed by BSG- and SSH-immobilized cells. Fermentation improved the content of proteins and ash, and decreased the content of fibers in all three support materials. In addition, the fermentation had favorable effect on in vitro dry matter digestibility and energy values of SSH and BSG. According to assessment of probiotic potential, L. paracasei demonstrated a favorable probiotic profile, exhibiting high resistance to simulated ruminant digestive tract and significant antioxidant and antimicrobial activity. CONCLUSIONS: The proposed strategy enables valorization of agro-industrial residues as value-added ruminant feed and simultaneous LA production. Following principles of circular economy, the developed process combines different raw materials and integrates them into a biorefinery process, improving the overall profitability and productivity. © 2019 Society of Chemical Industry.


Assuntos
Ração Animal/análise , Resíduos Industriais/análise , Ácido Láctico/metabolismo , Lactobacillus paracasei/metabolismo , Probióticos/análise , Agricultura , Ração Animal/microbiologia , Animais , Fermentação , Lactobacillus paracasei/crescimento & desenvolvimento , Melaço/análise , Melaço/microbiologia , Rúmen/metabolismo , Ruminantes , Solanum tuberosum/metabolismo , Solanum tuberosum/microbiologia
9.
Int J Mol Sci ; 20(7)2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30935131

RESUMO

Bacterial cell wall hydrolases, including amidases and peptidases, play a critical role in peptidoglycan turnover during growth, impacting daughter cell separation, and cell death, through autolysis. When exploring the regulation of protein expression across the growth cycle of an acid-resistant strain of Lactobacillus paracasei, GCRL 46, we observed temporal up-regulation of proteins in the 40⁻45 kDa molecular weight range for whole-cell extracts when culturing in fermenters at a controlled pH of 4.0 versus optimum growth pH of 6.3. Up-regulation of proteins in this size range was not detected in SDS-PAGE gels of the cytosolic fraction, but was routinely detected following growth at low pH in whole cells and cell debris obtained after bead beating and centrifugation, indicating a cell surface location. N-terminal sequencing and in silico analyses showed sequence similarity with proteins in the L. casei group (L. casei, L. paracasei and L. rhamnosus) which were variously annotated as uncharacterized proteins, surface antigens, possible TrsG proteins, CHAP (cysteine, histidine-dependent amidohydrolases/peptidases)-domain proteins or putative peptidoglycan d,l-endopeptidase due to the presence of a CwlO domain. This protein is a homologue of the p40 (Msp2) secreted protein of L. rhamnosus LGG, which is linked to probiotic functionality in this species, and is phylogenetically related to structurally-similar proteins found in Enterococcus, Streptococcus and Bifidobacterium species, including the glucan-binding (GbpB), surface antigen (SagA) proteins detected in pathogenic group A streptococci species as secreted, immunoglobulin-binding (SibA) proteins (also named PcsB). Three-dimensional (3D) modelling predicted structural similarities in the CHAP proteins from the L. casei group and streptococcal species, indicating retention of overall architecture despite sequence divergence, and an implied retention of function during evolution. A phylogenetically-related hydrolase also contained the CwlO domain with a NLPC_P60 domain, and showed similar overall but distinct architecture to the CHAP proteins. We concluded that the surface-located, CHAP protein in L. casei is up-regulated during long-term exposure to acidic conditions during growth but not during acid shock.


Assuntos
Proteínas de Bactérias/metabolismo , Lactobacillus paracasei/metabolismo , Streptococcus/metabolismo , Antígenos de Superfície/metabolismo , Bifidobacterium/metabolismo , Eletroforese em Gel de Poliacrilamida , Enterococcus/metabolismo , Concentração de Íons de Hidrogênio , Filogenia
10.
Int J Food Sci Nutr ; 70(8): 950-958, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30969137

RESUMO

Coeliac disease is an increasingly recognised pathology, induced by the ingestion of gluten in genetically predisposed patients. Undigested gliadin peptide can induce adaptive and innate immune response that unleash the typical intestinal mucosal alterations. A growing attention is paid to alternative therapeutic approaches to the gluten-free diet: one of these approaches is the use of probiotics and/or postbiotics. We performed lactic fermentation of rice flour with and without pH control, using Lactobacillus paracasei CBA L74 as fermenting strain. We evaluated bacterial growth, lactic acid production during fermentation and gliadin peptide P31-43 entrance in CaCo-2 cells with and without pH control. When pH control was applied no differences were observed in terms of bacterial growth; on the contrary, lactic acid production was greater, as expected. Both samples could inhibit the P31-43 entrance in CaCo-2 cells but the effect was significantly greater for samples obtained when the pH control was applied.


Assuntos
Células Epiteliais/metabolismo , Fermentação , Gliadina/metabolismo , Concentração de Íons de Hidrogênio , Oryza/microbiologia , Fragmentos de Peptídeos/metabolismo , Células CACO-2 , Doença Celíaca/tratamento farmacológico , Doença Celíaca/prevenção & controle , Dieta Livre de Glúten , Hipersensibilidade Alimentar/prevenção & controle , Alimento Funcional , Gliadina/antagonistas & inibidores , Glutens , Humanos , Ácido Láctico/metabolismo , Lactobacillus paracasei/metabolismo , Oryza/metabolismo , Fragmentos de Peptídeos/antagonistas & inibidores
11.
Food Microbiol ; 82: 541-550, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31027817

RESUMO

Amidst the rising popularity of craft beers, it would be opportune to develop a novel, unfiltered and unpasteurized sour beer with high probiotic live counts. However, as beer typically contains hop iso-α-acids that prevent the growth and survival of probiotic lactic acid bacteria, the use of suitable fermentation strategies is crucial. The growth, and survival of the probiotic bacterium, Lactobacillus paracasei L26, were assessed during a 10-day co-fermentation period with a brewer's yeast, Saccharomyces cerevisiae S-04, in unhopped wort. Isomerized hop extract was added prior to storage of the beers at 25 °C and 5 °C. During co-fermentation in unhopped wort, L. paracasei L26 maintained high viable cell counts above 8 Log CFU/mL, indicating species compatibility with the yeast. The majority of fermentable sugars were attenuated by S. cerevisiae S-04, with a concomitant production of alcohols and esters. Significant amounts of lactic acid were produced by L. paracasei L26 (P < 0.05). During storage with added isomerized hop extract, maximal probiotic viability enhancing effects were observed in the presence of live S. cerevisiae S-04, in combination with refrigeration. The results suggest that beers could be a vehicle for probiotic delivery under appropriate conditions. This was the first study demonstrating the feasibility of utilizing probiotic lactobacilli as starter cultures in beer brewing.


Assuntos
Cerveja/microbiologia , Lactobacillus paracasei/fisiologia , Probióticos , Saccharomyces cerevisiae/fisiologia , Cerveja/análise , Ésteres/análise , Fermentação , Concentração de Íons de Hidrogênio , Ácido Láctico/análise , Lactobacillus paracasei/crescimento & desenvolvimento , Lactobacillus paracasei/metabolismo , Interações Microbianas , Viabilidade Microbiana , Probióticos/metabolismo , Refrigeração , Saccharomyces cerevisiae/metabolismo , Açúcares/análise
12.
World J Microbiol Biotechnol ; 35(4): 66, 2019 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-30941513

RESUMO

It is widely assumed that bacterial resistance will be acquired when bacteria are exposed to long-term sublethal concentrations of antibiotics. The objective of this study was to evaluate the ability of two bacterial strains [Lactobacillus plantarum (18A) and Lactobacillus paracasei (18C)] isolated from the fuel ethanol industry to acquire bacterial resistance during long-term (≥ 14 days) exposure to sublethal concentrations of penicillin G and virginiamycin. Neither strain acquired resistance to virginiamycin after 69 days of exposure, but both strains did acquire resistance to penicillin G after 18 days. Strain 18A appeared to acquire resistance to a penicillin G and virginiamycin mixture after 7 days of exposure, but the incubation period was not long enough to verify. These results indicate that antibiotic resistance in two common Lactobacillus strains does not develop from sublethal exposure to virginiamycin after 69 days of exposure, but resistance can be developed with sublethal exposure to penicillin G.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/metabolismo , Farmacorresistência Bacteriana/efeitos dos fármacos , Etanol/metabolismo , Bactérias/crescimento & desenvolvimento , Bactérias/isolamento & purificação , Biocombustíveis , Fermentação , Lactobacillus paracasei/efeitos dos fármacos , Lactobacillus paracasei/crescimento & desenvolvimento , Lactobacillus paracasei/isolamento & purificação , Lactobacillus paracasei/metabolismo , Lactobacillus plantarum/efeitos dos fármacos , Lactobacillus plantarum/crescimento & desenvolvimento , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/metabolismo , Testes de Sensibilidade Microbiana , Modelos Teóricos , Penicilina G/farmacologia , Virginiamicina/farmacologia
13.
N Biotechnol ; 51: 8-13, 2019 Jul 25.
Artigo em Inglês | MEDLINE | ID: mdl-30716417

RESUMO

Rayon filaments composed of regenerated cellulose are used as reinforcement materials in tires and to a lower extent in the clothing industry as personal protective equipment e.g. flame retardant cellulosic based materials. After use, these materials are currently transferred to landfills while chemical degradation does not allow the recovery of the cellulose (as glucose) nor the separation of the high valuable flame-retardant pigment. In this study, rayon fibers were enzymatically hydrolyzed to allow recovery of glucose and valuable additives. The glucose was successfully used as carbon source for the production of high value compounds such as itaconic acid, lactic acid and chitosan. 14.2 g/L of itaconic acid, 36.5 g/L of lactic acid and 39.2 g/L of chitosan containing biomass were produced from Escherichia coli, Lactobacillus paracasei and Aspergillus niger, respectively, comparable to yields obtained when using commercial glucose as carbon source.


Assuntos
Carbono/metabolismo , Celulose/metabolismo , Quitosana/metabolismo , Glucose/metabolismo , Ácido Láctico/biossíntese , Succinatos/metabolismo , Aspergillus niger/metabolismo , Biomassa , Biotecnologia , Carbono/química , Celulose/química , Quitosana/química , Escherichia coli/metabolismo , Glucose/química , Ácido Láctico/química , Lactobacillus paracasei/metabolismo , Succinatos/química , Resíduos
14.
J Dairy Res ; 86(1): 120-128, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30729899

RESUMO

The aim of this work was to evaluate the impact of two factors on the ripening profiles of hard cooked cheeses: (F1) the growth medium for the primary and adjunct cultures, constituted by autochthonous strains: Lactobacillus helveticus 209 (Lh209) and Lactobacillus paracasei 90 (Lp90), respectively, and (F2) the addition of L. paracasei Lp90 as adjunct culture. Four types of cheeses were made: W and M cheeses in which only Lh209 was added after its growth in whey and MRS, respectively; Wa and Ma cheeses in which both strains (Lh209 and Lp90) were added after their growth in whey and MRS, respectively. Physicochemical and microbial composition, proteolysis and profiles of organic acids and volatile compounds were analyzed. According to the methodology of the cultures preparation, W and Wa cheeses showed a higher level of secondary proteolysis and lower level of primary proteolysis (P < 0·05), lower content of citric and acetic acids and higher amount of propionic acid (P < 0·05), in comparison with M and Ma cheeses. The incorporation of Lp90 increased the secondary proteolysis (P < 0·05), decreased the citric acid (P < 0·05), and increased the propionic acid only when was added after their growth in whey (P < 0·05). Both factors significantly modified the percentages of the volatile compounds grouped in chemical families; in addition, for the half of the compounds detected, significant differences were found. Based on the obtained results, the use of Lp90 as an adjunct in hard cooked cheeses, and the preincubation of the cultures in whey are strategies to accelerate the cheese ripening and to enhance the production of some characteristic compounds of this type of cheeses, such as propan-2-one, hexan-2-one, 2- and 3-methyl butanal, heptan-2-ol, acetic and 3-methylbutanoic acids and 3-hydroxy butan-2-one.


Assuntos
Queijo/análise , Queijo/microbiologia , Tecnologia de Alimentos/métodos , Lactobacillus helveticus/crescimento & desenvolvimento , Lactobacillus paracasei/crescimento & desenvolvimento , Ácido Acético/análise , Carboidratos/análise , Fenômenos Químicos , Ácido Cítrico/análise , Meios de Cultura , Fermentação , Manipulação de Alimentos/métodos , Concentração de Íons de Hidrogênio , Lactobacillus helveticus/metabolismo , Lactobacillus paracasei/metabolismo , Propionatos , Proteólise , Compostos Orgânicos Voláteis/análise
15.
J Sci Food Agric ; 99(9): 4200-4210, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30767231

RESUMO

BACKGROUND: Equol is a major isoflavone metabolite, and equol-producing bacteria have been isolated and characterized; however, fermentation has been performed with soybean-based products as substrates. Pueraria lobata has been reported as a plant with higher content of isoflavones. RESULTS: The genome of new equol-producing bacteria, Lactobacillus paracasei JS1, was analyzed. Also, the effect of P. lobata extract fermented with L. paracasei JS1 (FPE) on the skin and intestinal immune response was examined. With gene expression analysis, it was proven that seven skin-related proteins, hyaluronan synthase-1, -2, -3, collagen, elastin, epidermal growth factor, and epidermal growth factor receptor were differentially expressed upon FPE treatment. The messenger RNA expression increased with treatment with the FPE, and a skin moisturizing effect was confirmed by a hematoxylin-eosin staining experiment. In addition, such an experiment showed that proinflammatory cytokines, tumor necrosis factor-α, cyclooxygenase-2, inducible nitric oxide synthase, interleukin-1ß, -4, and -6, were reduced in large intestine when treated with FPE. CONCLUSION: L. paracasei JS1 has the ability to produce equol having beneficial effects on the skin. Moreover, FPE also has an inhibitory effect on inflammation cytokines in the large intestine. Thus, the novel and edible equol-producing L. paracasei JS1 and FPE have thepotential to be developed as nutricosmetic resources. © 2019 Society of Chemical Industry.


Assuntos
Equol/metabolismo , Enteropatias/prevenção & controle , Lactobacillus paracasei/metabolismo , Probióticos/administração & dosagem , Dermatopatias/prevenção & controle , Animais , Colágeno/genética , Colágeno/metabolismo , Elastina/genética , Elastina/metabolismo , Fermentação , Humanos , Hialuronan Sintases/genética , Hialuronan Sintases/metabolismo , Interleucina-1beta/genética , Interleucina-1beta/metabolismo , Enteropatias/genética , Enteropatias/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Dermatopatias/genética , Dermatopatias/metabolismo
16.
Ultrason Sonochem ; 51: 315-324, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30322762

RESUMO

Herein the effect of low intensity ultrasound on the fermentation of skim milk medium by Lactobacillus paracasei were investigated to obtain optimum ultrasonic conditions for the highest yield of yoghurt peptides. The results showed that the fermented skim milk medium treated with ultrasound with its seed culture without ultrasonic treatment was an optimum scheme. In this scheme with the ultrasonic conditions of 28 kHz, ultrasonic pulsed model of on-time 100 s and off-time 10 s, 100 W/L for the treatment time of 30 min after the fermentation time of 9 h, the peptide content in the fermented skim milk media increased by 49.5% and the viable cells in the same media increased by 43.5% compared with those in the untreated samples. By response surface methodology (RSM) analysis and its verification experiments, a reasonably accurate empirical model was established for investigating and predicting the relationship between skim milk concentration, ultrasonic treatment time, power and the yield of yoghurt peptides. The former two parameters 12.6% w/v and 35 min were taken in the verification experiments in which the peptide content of the fermented media reached 5.9 mg/mL with an increase by 64.23% and the peptide yield was 14.2%, similar to its theoretical value of 14.6% according to the empirical model. The comparison of extracellular enzyme activities in the fermented skim milk media between with and without ultrasonic treatment under the conditions in the optimum scheme indicated that the mechanism of the ultrasound-activated peptide content increment might be the extracellular enzyme activities immediately activated by the ultrasound, effect of which would disappear in the progress of fermentation after the ultrasound was removed.


Assuntos
Fermentação , Lactobacillus paracasei/metabolismo , Leite/microbiologia , Peptídeos/metabolismo , Ondas Ultrassônicas , Iogurte/microbiologia , Animais , Biomassa , Espaço Extracelular/enzimologia , Concentração de Íons de Hidrogênio , Lactobacillus paracasei/citologia , Lactobacillus paracasei/crescimento & desenvolvimento
17.
Probiotics Antimicrob Proteins ; 11(1): 264-272, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-29119465

RESUMO

The aims of the present study were to develop and evaluate different formulations of probiotic and synbiotic sorbets produced with jussara (Euterpe edulis) pulp, polydextrose, Lactobacillus acidophilus LA3, and Lactobacillus paracasei BGP1. The pasteurized jussara pulp presented high content of phenolic compounds, especially anthocyanins, which were not inhibitory to the probiotics used in this study. The levels of polyphenols and anthocyanins present in the sorbets were also high and kept stable for 120 days, as well as the populations of both probiotics. On the other hand, probiotic populations reduced ca. 4 log CFU/g when exposed to simulated gastrointestinal fluids. Altogether, the sorbets produced in this study showed interesting results, indicating the viability on producing functional foods with probiotics, prebiotics, and other components that are rich in polyphenols, such as jussara pulp. The combination of these elements can improve the health beneficial effects of these compounds and provide important advantages to the intestinal microbiota of consumers.


Assuntos
Euterpe/química , Trato Gastrointestinal/microbiologia , Lactobacillus acidophilus/química , Lactobacillus paracasei/química , Extratos Vegetais/química , Prebióticos/análise , Probióticos/química , Simbióticos/análise , Armazenamento de Medicamentos , Humanos , Lactobacillus acidophilus/metabolismo , Lactobacillus paracasei/metabolismo , Modelos Biológicos , Resíduos/análise
18.
J Med Food ; 22(3): 257-263, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30543483

RESUMO

Compound K (CK) is a metabolite of a saponin in Panax ginseng, formed from ginsenoside, a triterpenoid glycoside, by human intestinal bacteria. Lactobacillus paracasei A221 isolated from fermented food can hydrolyze (deglycosylate) the main ginsenoside, ginsenoside Rb1, and generate CK. However, the pharmacokinetics of L. paracasei A221 fermented ginseng (FG) and nonfermented ginseng (NFG) have not been investigated so far. The aim of this study was to investigate the pharmacokinetics of CK after oral administration of single doses of FG and NFG in healthy Japanese adults. An open-label, randomized, single-dose, two-period, crossover study was conducted in 12 Japanese healthy volunteers (five men and seven women, aged 40-60 years). All subjects were equally allocated into two groups and administered tablets containing FG or NFG. Until 24 h after the administration, blood samples were sequentially collected, plasma concentrations of CK were measured, and the pharmacokinetic parameters were calculated. We also expected restoration of decreased testosterone level as one of the beneficial effects of FG and measured plasma total testosterone concentrations in male volunteers. The means of Tmax, Cmax, and area under the concentration-time curve (AUC) were significantly different between the two groups. In the FG group, AUC0-12h (ng h/mL) and AUC0-24h (ng h/mL) were, respectively, 58.3- and 17.5-fold higher than those in the NFG group. Moreover, mean testosterone concentration in the FG group significantly increased 24 h after administration. These results showed that the main ginsenoside metabolite of ginseng, CK, produced by L. paracasei A221 has potential utility in health maintenance in healthy middle-aged and old Japanese adults.


Assuntos
Ginsenosídeos/farmacocinética , Lactobacillus paracasei/metabolismo , Panax/microbiologia , Adulto , Estudos Cross-Over , Feminino , Fermentação , Ginsenosídeos/administração & dosagem , Ginsenosídeos/sangue , Humanos , Japão , Masculino , Pessoa de Meia-Idade , Panax/química , Testosterona/sangue
19.
Appl Biochem Biotechnol ; 187(3): 753-769, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30058018

RESUMO

The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4 g L-1 of LA, with a yield of 0.89 g g-1, a productivity of 1.49 g L-1 h-1, and an optical purity greater than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9 g L-1) compared to parent strain (107.1 g L-1). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate.


Assuntos
Adaptação Fisiológica , Agricultura , Evolução Molecular , Indústrias , Ácido Láctico/biossíntese , Lactobacillus paracasei/metabolismo , Técnicas de Cultura Celular por Lotes , Fermentação , Lactobacillus paracasei/fisiologia
20.
J Dairy Sci ; 101(12): 10664-10674, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30292551

RESUMO

We investigated in vitro and in vivo antioxidant activity of Lactobacillus paracasei ssp. paracasei YBJ01 (LPSP-YBJ01) isolated and identified from fermented yogurt. Strain LPSP-YBJ01 had stress tolerance against acidity, bile salt, and osmotic pressure. Five in vitro antioxidant assays were used to evaluate antioxidant activity of LPSP-YBJ01, which could scavenge free radicals (2,2-diphenyl-1-picrylhydrazyl and hydroxyl) and superoxide anion in vitro. In addition, strain LPSP-YBJ01 had stronger antilipid peroxidation activity and weak reducing power in vitro. We measured in vivo antioxidant activity of LPSP-YBJ01 in an oxidation mouse model induced by d-galactose injection. Strain LPSP-YBJ01 significantly increased serum superoxide dismutase (SOD), glutathione peroxidase, and total-antioxidant capability, and inhibited generation of malondialdehyde in a dose-dependent manner. In addition, strain LPSP-YBJ01 also increased the hepatic and splenic protein expressions of some antioxidant enzymes such as catalase, Cu/Zn-SOD, and Mn-SOD in mice treated with d-galactose. Thus, LPSP-YBJ01 had antioxidant activity in vitro and in vivo and may be a useful probiotic.


Assuntos
Galactose/efeitos adversos , Lactobacillus paracasei/metabolismo , Estresse Oxidativo , Animais , Antioxidantes/farmacologia , Catalase/metabolismo , Bovinos , Fermentação , Galactose/metabolismo , Glutationa Peroxidase/metabolismo , Lactobacillus paracasei/isolamento & purificação , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Probióticos/farmacologia , Superóxido Dismutase/metabolismo , Iogurte/microbiologia
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