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1.
Food Chem ; 339: 127985, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-32920305

RESUMO

There is limited research focusing on the effects of human gut microbiota on the oral bioaccessibility and intestinal absorption of pesticide residues in food. In the present study, we use a modified setup of the Simulator of the Human Intestinal Microbial Ecosystem for the determination of pesticide residue bioaccessibility in Chaenomeles speciosa, and a Caco-2 cell model of human intestinal absorption. Results showed that gut microbiota played a dual role based their effects on contaminant release and metabolism in the bioaccessibility assay, and Lactobacillus plantarum was one of key bacterial species in the gut microbiota that influenced pesticide stability significantly. The addition of L. plantarum to the system reduced the relative amounts (by 11.40-86.51%) of six pesticides. The interaction between the food matrix and human gut microbiota led to different absorption rates, and the barrier effects increased with an increase in incubation time.


Assuntos
Microbioma Gastrointestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Praguicidas/farmacologia , Rosaceae/química , Bactérias/metabolismo , Células CACO-2 , Humanos , Mucosa Intestinal/citologia , Mucosa Intestinal/microbiologia , Lactobacillus plantarum/efeitos dos fármacos , Lactobacillus plantarum/isolamento & purificação , Neonicotinoides/metabolismo , Neonicotinoides/farmacologia , Nitrocompostos/metabolismo , Nitrocompostos/farmacologia , Compostos Organotiofosforados/metabolismo , Compostos Organotiofosforados/farmacologia , Praguicidas/química , Praguicidas/metabolismo , Rosaceae/metabolismo , Tiametoxam/metabolismo , Tiametoxam/farmacologia
2.
J Appl Microbiol ; 129(5): 1374-1388, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32356362

RESUMO

AIMS: To determine the mechanism underlying the serum cholesterol reduction effect by probiotics isolated from local fermented tapioca (Tapai). METHODS AND RESULTS: Lactic acid bacteria strains were isolated and examined for acid tolerance, bile salt resistance and hypocholesterolemic properties. Among the isolates, Lactobacillus plantarum TAR4 showed the highest cholesterol reduction ability (48·01%). The focus in the in vivo trial was to elucidate the cholesterol balance from findings pertaining to serum cholesterol reduction in rat model fed with high fat diet via oral administration. Rats fed with high-cholesterol diet supplemented with Lact. plantarum TAR4 showed significant reduction in serum total cholesterol (29·55%), serum triglyceride (45·31%) and liver triglyceride (23·44%) as compared to high-cholesterol diet (HCD) group. There was a significant increment in faecal triglyceride (45·83%) and faecal total bile acid (384·95%) as compared to HCD group. CONCLUSIONS: The findings showed that probiotic Lact. plantarum TAR4 supplementation reduced the absorption of bile acids for enterohepatic recycling and increased the catabolism of cholesterol to bile acids and not by suppressing the rate of cholesterol synthesis. SIGNIFICANCE AND IMPACT OF STUDY: Probiotic supplements could provide a new nonpharmacological alternative to reduce cardiovascular risk factors.


Assuntos
Colesterol/metabolismo , Suplementos Nutricionais , Alimentos e Bebidas Fermentados/microbiologia , Homeostase , Probióticos/administração & dosagem , Animais , Ácidos e Sais Biliares/metabolismo , Dieta Hiperlipídica/efeitos adversos , Homeostase/efeitos dos fármacos , Lactobacillales/classificação , Lactobacillales/isolamento & purificação , Lactobacillales/metabolismo , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Manihot , Probióticos/farmacologia , Ratos , Triglicerídeos/metabolismo
3.
J Dairy Sci ; 103(7): 5893-5905, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32389475

RESUMO

Jiaoke is a traditional Mongolian fermented dairy product that is nutritious and has a unique taste. It is made from the fat separated from fermented milk. In this study, we collected 24 jiaoke samples from the Xilingol region of Inner Mongolia. The microbiota composition of the collected samples was analyzed using 16S rRNA small-molecule real-time sequencing, and the lactic acid bacteria (LAB) population was enumerated and isolated by laboratory culture techniques. We used an electronic tongue device to assess the taste quality of the products. One hundred fifty LAB isolates (5 genera and 14 species) were recovered and identified by 16S rRNA sequencing across all samples. Lactococcus lactis and Lactobacillus plantarum accounted for 51.33% and 10.67% of the total isolates, respectively. The small-molecule real-time sequencing of full-length 16S rRNAs revealed an overall bacterial microbiota composition of 10 phyla, 121 genera, and 186 species, largely represented by sequences of Lactococcus (68.46%) and Lactococcus lactis (52.92%) at the genus and species levels, respectively. The electronic tongue analysis revealed that the sweetness, bitterness, sourness, and saltiness of jiaoke varied greatly between samples. The presence of Lactococcus lactis correlated positively with bitter aftertaste; the presence of Lactococcus piscium correlated positively with umami and negatively with astringent and bitter aftertastes; and the presence of Lactobacillus helveticus correlated positively with sourness and negatively with other taste qualities. These results suggest that the microbiota composition and product taste are closely related. The novel LAB strains collected in this work represent valuable natural microbial resources.


Assuntos
Bactérias/isolamento & purificação , Produtos Fermentados do Leite/microbiologia , Microbiota , China , Microbiologia de Alimentos , Lactobacillales/isolamento & purificação , Lactobacillus plantarum/isolamento & purificação , Lactococcus lactis/isolamento & purificação , RNA Bacteriano/análise , RNA Ribossômico 16S/análise
4.
Benef Microbes ; 11(2): 163-173, 2020 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-32131607

RESUMO

This study reports the probiotic attributes of Lactobacillus strains isolated from chicken faeces and mainly their capabilities to prevent infectious diseases and improve chicken production performance. Thus, 22 Lactobacillus strains were isolated from 50 chickens' faeces samples and assessed for their resistance to gastric acidity (pH 0.5, 1, 1.5, 2 and 2.5), tolerance to bile salts, adherence to broiler intestinal cells and antibacterial activity. These in vitro screening analyses revealed Lactobacillus plantarum S22 and L. plantarum S27 as the only strains capable to survive at pH 2.0 in MRS broth (log10 cfu/ml=5.02 and 8.46 log respectively), while the remaining strains were not resistant to pH≤2.0. Similarly, 21 strains were resistant to bile at 0.5% (log10 cfu/ml=0.09-3.32 log), but only Lactobacillus fermentum S26, L. plantarum S22 and L. plantarum S27 were able to grow in the presence of 0.1% (w/v) bile (8.23±0.15; 8.39±0.17 and 8.57±0.07 respectively). Most of these isolates (19/22) were active against Escherichia coli ATCC 25922, E. coli SL2016 and Salmonella enterica CIP 81-3. Lactic acid is likely the main antibacterial compound produced since the neutralised supernatant was devoid of any antibacterial activity. In vitro characterisation of these 22 novel strains, based on the aforementioned criteria revealed L. plantarum S27 as the most suitable strain for in vivo analyses. To this end, this strain was assessed for its sensitivity to different antibiotics and adhesion to poultry intestinal cells to ascertain it probiotic attributes. The administration of L. plantarum S27 to the chicks at 109 cfu/ml permitted to improve the animal food intake and weight. Taken together, data from in vitro and in vivo analyses indicated that L. plantarum S27 might be a worthy probiotic for chickens rather than adding antibiotics to animals feeding.


Assuntos
Fezes/microbiologia , Lactobacillus plantarum/isolamento & purificação , Lactobacillus/isolamento & purificação , Probióticos/administração & dosagem , Animais , Antibacterianos/farmacologia , Aderência Bacteriana , Ácidos e Sais Biliares/química , Galinhas/crescimento & desenvolvimento , Galinhas/microbiologia , Meios de Cultura/química , Células Epiteliais/microbiologia , Escherichia coli/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Intestinos/citologia , Ácido Láctico/metabolismo , Lactobacillus/classificação , Lactobacillus plantarum/efeitos dos fármacos , Lactobacillus plantarum/fisiologia , Masculino
5.
J Appl Microbiol ; 128(6): 1764-1775, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32027448

RESUMO

AIMS: The purpose of this study was to isolate Lactobacillus from gastrointestinal tract of healthy postweaning piglets and investigate its synergistic antimicrobial and probiotic effects with ZnO nanoparticles (nZnO). METHODS AND RESULTS: Of the 128 isolates, Lactobacillus plantarum BLPL03 was selected based on its excellent acid and bile salt tolerance properties. Lactobacillus plantarum BLPL03 was sensitive to ß-lactams, macrolides, amphenicols and cephalosporins, whereas it displayed the steady resistance to aminoglycosides, tetracyclines, quinolones and peptide antibiotics. In vitro analysis of antibacterial activities showed that L. plantarum BLPL03 inhibited the four common food-borne pathogenic bacteria including Escherichia coli O157:H7 CMCC 44828, Salmonella Typhimurium ATCC 13311, Staphylococcus aureus CMCC 26003 and Listeria monocytogenes CMCC 54007 in synergy with nZnO. Furthermore, the quantitative polymerase chain reaction test demonstrated that the combined administration of L. plantarum BLPL03 fermentation liquor (LFL) and nZnO synergistically elevated the faecal number of Bifidobacterium by 73·19-fold, and reduced the two potential enteropathogenic bacteria Enterobacteriaceae and Clostridium perfringens in mice challenged with Salm. Typhimurium. Finally, dietary supplementation with low dose of nZnO (20 mg kg-1 ) when combined with LFL administration enhanced final body weight, fur appearance and average daily gain, and decreased feed conversion ratio and diarrhoea incidence in weaned piglets. The faecal Bifidobacterium and Lactobacillus of piglets were dramatically enhanced by 81·96- and 3·15-fold, respectively, after administration of a mixture of nZnO and LFL. Meanwhile, combination of nZnO with LFL resulted in low levels of Bacteroides, Enterococcus, and Enterobacteriaceae. CONCLUSIONS: A combination of nZnO and LFL exhibits potential health-benefit properties for the control of gut microbial composition by their synergistic antimicrobial and probiotic effects. SIGNIFICANCE AND IMPACT OF THE STUDY: This study may provide a potential nutritional strategy to improve performance and gut health of animals with gut microbiota disorders caused by pathogen infections and weanling, and so on.


Assuntos
Antibacterianos/farmacologia , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/fisiologia , Probióticos/farmacologia , Óxido de Zinco/farmacologia , Animais , Antibacterianos/administração & dosagem , Suplementos Nutricionais , Sinergismo Farmacológico , Fezes/microbiologia , Microbioma Gastrointestinal/efeitos dos fármacos , Trato Gastrointestinal/microbiologia , Lactobacillus plantarum/efeitos dos fármacos , Camundongos , Nanopartículas/administração & dosagem , Nanopartículas/química , Probióticos/administração & dosagem , Suínos/microbiologia , Desmame , Óxido de Zinco/administração & dosagem , Óxido de Zinco/química
6.
Mol Biol Rep ; 47(3): 1871-1881, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32006197

RESUMO

A recent spike in demand for chemical preservative free food has derived the scientific community to develop natural ways of food preservation. Therefore, bio-preservation could be considered as the great alternative over chemical ones owing to its potential to increase shelf-life and nutritional values of foodstuffs. In the present study, lactic acid producing bacterial species were isolated from rice rinsed water and identified by 16S rRNA gene sequencing as Lactobacillus plantarum BCH-1 (KX388380) and Lactobacillus coryniformis BCH-4 (KX388387). Antifungal metabolites from both Lactobacillus species were extracted by polarity-based solvents in which ethyl acetate showed remarkable antifungal activity against Aspergillus flavus and Aspergillus fumigatus by disc diffusion assay. Different organic acids and fatty acids have been identified by reversed-phase high-performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-MS) analysis, respectively. Lactic acid and citric acid were the major organic acids found in ethyl acetate fractions of L. plantarum and L. coryniformis, respectively. Similarly, 9,12-otadecadienoic acid (Z,Z)-methyl ester and hexadecanoic acid, methyl ester were the major fatty acids found in n-hexane fractions of L. plantarum and L. coryniformis respectively. Moreover, the isolation of novel antifungal metabolites from locally isolated Lactobacillus species was focused and it was revealed that organic acids are important contributors towards antifungal potential. A novel fatty acid (i.e. 12-hydroxydodecanoic acid) has also been explored and found as potential metabolite against filamentous fungi. Conclusively, various metabolites isolated from non-dairy source showed antifungal activity especially against Aspergillus species. Hence, these metabolites have been considered as a good choice for bio-preservation.


Assuntos
Antifúngicos/farmacologia , Aspergillus/efeitos dos fármacos , Lactobacillus/classificação , Metabolômica/métodos , Oryza/microbiologia , Acetatos/isolamento & purificação , Antifúngicos/isolamento & purificação , Aspergillus/crescimento & desenvolvimento , Cromatografia de Fase Reversa , DNA Fúngico/genética , DNA Ribossômico/genética , Conservação de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Lactobacillus/química , Lactobacillus/genética , Lactobacillus/isolamento & purificação , Lactobacillus plantarum/química , Lactobacillus plantarum/genética , Lactobacillus plantarum/isolamento & purificação , Ácidos Láuricos/isolamento & purificação , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
7.
Sci Rep ; 10(1): 1165, 2020 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-31980710

RESUMO

Bile acid (BA) signatures are altered in many disease states. BA metabolism is an important microbial function to assist gut colonization and persistence, as well as microbial survival during gastro intestinal (GI) transit and it is an important criteria for potential probiotic bacteria. Microbes that express bile salt hydrolase (BSH), gateway BA modifying enzymes, are considered to have an advantage in the gut. This property is reported as selectively limited to gut-associated microbes. Food-associated microbes have the potential to confer health benefits to the human consumer. Here, we report that food associated Lactobacillus plantarum strains are capable of BA metabolism, they can withstand BA associated stress and propagate, a recognised important characteristic for GIT survival. Furthermore, we report that these food associated Lactobacillus plantarum strains have the selective ability to alter BA signatures in favour of receptor activation that would be beneficial to humans. Indeed, all of the strains examined showed a clear preference to alter human glycol-conjugated BAs, although clear strain-dependent modifications were also evident. This study demonstrates that BA metabolism by food-borne non-pathogenic bacteria is beneficial to both microbe and man and it identifies an evolutionary-conserved characteristic, previously considered unique to gut residents, among food-associated non-pathogenic isolates.


Assuntos
Ácidos e Sais Biliares/metabolismo , Microbiologia de Alimentos , Lactobacillus plantarum/metabolismo , Animais , Ácidos e Sais Biliares/farmacologia , Brassica/microbiologia , Pão/microbiologia , Queijo/microbiologia , Relação Dose-Resposta a Droga , Fermentação , Humanos , Lactobacillus plantarum/efeitos dos fármacos , Lactobacillus plantarum/isolamento & purificação , Olea/microbiologia , Probióticos , Saliva/microbiologia , Suínos
8.
Int J Biol Macromol ; 144: 938-946, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31672637

RESUMO

Various industries highly regard the functionalities and bioactivities of bacterial polysaccharides. We aimed to characterize the exopolysaccharide (EPS) produced by novel probiotic Lactobacillus plantarum C70 (accession number KX881779) isolated from camel milk and to investigate its bioactivities and rheological properties. EPS-C70 had a weight-average molecular weight (Mw) of 3.8 × 105 Da. Arabinose (13.3%), mannose (7.1%), glucose (74.6%), and galactose (5.0%) were the major monosaccharides constituents. EPS-C70 had two endothermic peaks at 76.95 °C and 158.76 °C corresponding to glass transition (Tg) and melting point (Tm), respectively. Zeta potential and particle size of EPS-C70 were -330.71 mV and 525.5 nm, respectively. DPPH and ABTS of EPS-C70 were 75.91% and 49.42% at 10 mg/mL concentration, respectively. The cytotoxic activities against colon cancer and breast cancer lines were 88.1% and 73.1% at concentration 10 mg/mL, respectively. EPS-C70 exhibited shear-thinning behaviour. Salts and pH values had a significant impact on the rheological properties of EPS-C70.


Assuntos
Camelus , Lactobacillus plantarum/metabolismo , Leite/microbiologia , Polissacarídeos Bacterianos/biossíntese , Polissacarídeos Bacterianos/farmacologia , Probióticos/metabolismo , Reologia , Animais , Benzotiazóis/química , Compostos de Bifenilo/química , Elasticidade , Inibidores de Glicosídeo Hidrolases/química , Inibidores de Glicosídeo Hidrolases/metabolismo , Inibidores de Glicosídeo Hidrolases/farmacologia , Lactobacillus plantarum/isolamento & purificação , Monossacarídeos/análise , Picratos/química , Polissacarídeos Bacterianos/química , Probióticos/isolamento & purificação , Ácidos Sulfônicos/química , Temperatura , Viscosidade , alfa-Amilases/antagonistas & inibidores , alfa-Glucosidases/metabolismo
9.
J Dairy Sci ; 103(2): 1223-1237, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31759592

RESUMO

Raw milk contains wide microbial diversity, composed mainly of lactic acid bacteria (LAB), which are used as probiotics in both human and animal husbandry. We isolated, characterized, and evaluated LAB from indigenous Bangladeshi raw milk to assess probiotic potential, including antagonistic activity (against Escherichia coli O157: H7, Enterococcus faecalis, Salmonella Typhimurium, Salmonella Enteritidis, and Listeria monocytogenes), survivability in simulated gastric juice, tolerance to phenol and bile salts, adhesion to ileum epithelial cells, auto- and co-aggregation, hydrophobicity, α-glucosidase inhibitory activity, and antibiotic susceptibility tests. The 4 most promising LAB strains showed probiotic potential and were identified as Lactobacillus casei, Lactobacillus plantarum (which produced plantaricin EF), Lactobacillus fermentum, and Lactobacillus paracasei. These strains inhibited all pathogens tested at various degrees, and competitively excluded pathogens with viable counts of 3.0 to 6.0 log cfu/mL. Bacteriocin, organic acids, and low-molecular-weight substances were mainly responsible for antimicrobial activity by the LAB strains. All 4 LAB strains were resistant to oxacillin and 3 were resistant to vancomycin and streptomycin, with multiple antibiotic resistance indices >0.2. After further in vivo evaluation, these LAB strains could be considered probiotic candidates with application in the food industry.


Assuntos
Lactobacillales/fisiologia , Leite/microbiologia , Probióticos , Animais , Bacteriocinas/metabolismo , Bovinos , Enterococcus faecalis/isolamento & purificação , Enterococcus faecalis/fisiologia , Feminino , Suco Gástrico/microbiologia , Cabras , Humanos , Lactobacillales/isolamento & purificação , Lactobacillus casei/isolamento & purificação , Lactobacillus casei/fisiologia , Lactobacillus fermentum/isolamento & purificação , Lactobacillus fermentum/fisiologia , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/fisiologia , Probióticos/farmacologia
10.
Nutrients ; 11(11)2019 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-31752138

RESUMO

Folate deficiencies are widespread around the world. Promoting consumption of folate-rich foods could be a sustainable option to alleviate this problem. However, these foods are not always available. Cereals, being a staple food, could contribute to folate intake. They are fermented prior to consumption in many African countries, and fermentation can modify the folate content. In Ethiopia, injera is a widely consumed fermented flat bread. The main drivers of its fermentation are lactic acid bacteria (LAB). The aim of this work was to isolate and identify folate-producing LAB from injera fermented dough and to evaluate their ability to increase folate status after depletion in a rat model. Among the 162 strains isolated from 60 different fermentations, 19 were able to grow on a folate-free culture medium and produced 1 to 43 µg/L (24 h, 30 °C incubation). The four highest folate producers belonged to the Lactobacillus plantarum species. The most productive strain was able to enhance folate status after depletion in a rat model, despite the relatively low folate content of the feed supplemented with the strain. Folate-producing L. plantarum strain has potential use as a commercial starter in injera production.


Assuntos
Grão Comestível/microbiologia , Alimentos e Bebidas Fermentados/microbiologia , Ácido Fólico/análise , Lactobacillales/isolamento & purificação , Lactobacillus plantarum/isolamento & purificação , Animais , Pão , Etiópia , Deficiência de Ácido Fólico , Microbiologia de Alimentos , Masculino , Ratos , Ratos Wistar
11.
Toxins (Basel) ; 11(11)2019 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-31683906

RESUMO

Lactobacillus plantarum, as a natural bio-preservative, has attracted a great deal of attention in recent years. In this study, 22 L. plantarum strains were tested against the aflatoxin-producing fungus, Aspergillus flavus; strain IAMU80070 showed the highest antifungal activity. At a concentration of 5 × 105 colony-forming units (CFU) mL-1, it completely inhibited A. flavus growth and decreased aflatoxin production by 93%. Furthermore, ultrastructural examination showed that IAMU80070 destroyed the cellular structure of hyphae and spores. To explore the inhibitory effect of IAMU80070 on A. flavus at the transcriptional level, transcriptome data were obtained and subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The aflatoxin biosynthetic process was the most significantly downregulated functional category, while genes implicated in the synthesis and organization of cell wall polysaccharides were upregulated. Quantitative real-time PCR results verified the credibility and reliability of the RNA sequencing data. These results provided insight into the transcriptome of A. flavus in response to the antagonistic effects of L. plantarum IAMU80070.


Assuntos
Aflatoxinas/biossíntese , Aspergillus flavus/crescimento & desenvolvimento , Lactobacillus plantarum/genética , Transcriptoma , Aspergillus flavus/metabolismo , Aspergillus flavus/ultraestrutura , Laticínios/microbiologia , Microbiologia de Alimentos , Perfilação da Expressão Gênica , Ontologia Genética , Lactobacillus plantarum/isolamento & purificação , Análise de Sequência de RNA , Esporos Fúngicos/crescimento & desenvolvimento , Esporos Fúngicos/metabolismo , Esporos Fúngicos/ultraestrutura , Transcriptoma/genética
12.
J Microbiol Biotechnol ; 29(12): 1925-1930, 2019 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-31635447

RESUMO

Carotenoids are organic pigments with antioxidant properties and are widespread in nature. Here, we isolated five microbes, each forming yellow-colored colonies and harboring C30 carotenoid biosynthetic genes (crtM and crtN). Thereafter, Lactobacillus plantarum subsp. plantarum KCCP11226, which showed the highest carotenoid production, was finally selected and the produced pigment was identified as C30 carotenoid 4,4'-diaponeurosporene. This strain exhibited the highest survival rate under oxidative stress and its carotenoid production was also enhanced after exposure to 7 mM H2O2. Moreover, it showed the highest ability to scavenge DPPH free radical. Our results suggested that L. plantarum subsp. plantarum KCCP11226, which produces 4,4'-diaponeurosporene as a natural antioxidant, may be a functional probiotic.


Assuntos
Antioxidantes/metabolismo , Carotenoides/metabolismo , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/metabolismo , Triterpenos/metabolismo , Genes Bacterianos/genética , Peróxido de Hidrogênio/efeitos adversos , Peróxido de Hidrogênio/farmacologia , Lactobacillus plantarum/genética , Probióticos
13.
J Med Food ; 22(12): 1208-1221, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31621475

RESUMO

This study determined the ameliorative effects of the novel microorganism, Lactobacillus plantarum CQPC02 (LP-CQPC02), fermented in soybean milk, on loperamide-induced constipation in Kunming mice. High-performance liquid chromatography revealed that LP-CQPC02-fermented soybean milk (LP-CQPC02-FSM) had six types of soybean isoflavones, whereas Lactobacillus bulgaricus-fermented soybean milk (LB-FSM) and unfermented soybean milk (U-FSM) only had five types of soybean isoflavones. LP-CQPC02-FSM also contained more total and active soybean isoflavones than LB-FSM and U-FSM. Results from mouse experiments showed that the defecation factors (quantity, fecal weight and water content, gastrointestinal transit ability, and time to first black stool) in the LP-CQPC02-FSM-treated mice were better than those in the LB-FSM- and U-FSM-treated mice. The serum and small intestinal tissue experiments showed that soybean milk increased the motilin, gastrin, endothelin, acetylcholinesterase, substance P, vasoactive intestinal peptide, and glutathione levels and decreased the somatostatin, myeloperoxidase, nitric oxide, and malondialdehyde levels compared with the constipated mice in the control group. The LP-CQPC02-FSM also showed better effects than those of LB-FSM and U-FSM. Further results showed that LP-CQPC02-FSM upregulated cuprozinc-superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), catalase (CAT), c-Kit, stem cell factor (SCF), glial cell-derived neurotrophic factor (GDNF), neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), and aquaporin-9 (AQP9) and downregulated the expression levels of transient receptor potential cation channel subfamily V member 1 (TRPV1), inducible nitric oxide synthase (iNOS), and aquaporin-3 (AQP3) in the constipated mice. LP-CQPC02-FSM increased the Bacteroides and Akkermansia abundances and decreased the Firmicutes abundance in the feces of the constipated mice and decreased the Firmicutes/Bacteroides ratio. This study confirmed that LP-CQPC02-FSM partially reversed constipation in mice.


Assuntos
Constipação Intestinal/terapia , Fermentação , Lactobacillus plantarum/metabolismo , Loperamida/efeitos adversos , Leite/metabolismo , Alimentos de Soja , Soja/metabolismo , Acetilcolinesterase/metabolismo , Animais , Aquaporina 3/metabolismo , Aquaporinas , Catalase/metabolismo , Constipação Intestinal/induzido quimicamente , Modelos Animais de Doenças , Endotelinas/metabolismo , Fezes/microbiologia , Feminino , Gastrinas/metabolismo , Trânsito Gastrointestinal , Mucosa Intestinal/metabolismo , Intestinos/patologia , Isoflavonas , Lactobacillus plantarum/isolamento & purificação , Malondialdeído/metabolismo , Camundongos , Motilina/metabolismo , Óxido Nítrico/metabolismo , Óxido Nítrico Sintase Tipo III/metabolismo , Proteínas Proto-Oncogênicas c-kit , Fator de Células-Tronco/metabolismo , Superóxido Dismutase/metabolismo , Superóxido Dismutase-1/metabolismo , Canais de Cátion TRPV/metabolismo
14.
J Food Biochem ; 43(10): e12837, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31608476

RESUMO

The development of yogurt flavor is a complicated and dynamic biochemical process. In addition to traditional starter cultures, adjunct cultures could also make significant contributions to the flavor profiles of yogurt. In the current study, two Lactobacillus plantarum strains (1-33 and 1-34) were isolated based on their abilities to produce acetaldehyde and diacetyl. In co-fermentation with traditional starters, these isolated strains were able to maintain viability without affecting the yogurt's acidification profiles. Furthermore, they positively influenced the aroma quality of the yogurt samples. They promoted the formation of volatile metabolites, especially acetaldehyde, diacetyl, and acetoin, which are recognized as characteristic compounds. The results of this work provide novel knowledge about the contributions of isolated strains on the flavor profiles of yogurt, which will help to improve the organoleptic properties of the final products. PRACTICAL APPLICATIONS: Using lactic acid bacteria (LAB) as adjunct cultures co-fermented with traditional yogurt starter cultures can increase the quantities of flavor compounds in yogurt. This study enriches our understanding of the effects of adjunct cultures on yogurt flavor. Researchers and manufacturers that specialize in yogurt making can use the results of this study to improve the aromatic profile and organoleptic quality of yogurt.


Assuntos
Aromatizantes/química , Lactobacillus plantarum/metabolismo , Iogurte/análise , Acetaldeído/análise , Acetaldeído/metabolismo , Animais , Bovinos , Diacetil/análise , Diacetil/metabolismo , Fermentação , Alimentos e Bebidas Fermentados/análise , Alimentos e Bebidas Fermentados/microbiologia , Aromatizantes/metabolismo , Humanos , Lactobacillus plantarum/genética , Lactobacillus plantarum/isolamento & purificação , Leite/metabolismo , Leite/microbiologia , Odorantes/análise , Paladar , Iogurte/microbiologia
15.
Microb Pathog ; 136: 103677, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31437574

RESUMO

Probiotics are microbial strains beneficial to human health if consumed in appropriate amounts. Their potential has recently led to a significant increase in research interest in their effects on the intestine, mainly by reinforcing the intestinal epithelium and modulating the gut microbiota. This study aimed to evaluate the probiotic features of Lactobacillus plantarum strain L15 based on adhesive properties for the inhibition of the adhesion of infectious pathogens. The molecular identification of the strain was performed from the sequencing of 16S ribosomal DNA with 27FYM and 1492R primers, and its probiotic features, including resistance to gastric juices, resistance to bile salts, and hydrophobicity were evaluated. The potential of Lactobacillus plantarum strain L15 to adhere to human adenocarcinoma intestinal cell line, Caco-2, as well as the auto and co-aggregation and anti-adherence activity against Escherichia coli were investigated. The results demonstrated that this strain has a desirable potential for passing through the low pH of the stomach and entering the intestines. Moreover, 54% hydrophobicity, 44% auto-aggregation, and 32% co-aggregation were observed for this strain. The adhesion level of Lactobacillus plantarum strain L15 to Caco-2 cells was 12%, and adhered lactobacilli cells were observed by scanning electron microscopy (SEM). Furthermore, this strain showed appropriate anti-adherence effects, including competition, inhibition, and replacement properties against Escherichia coli. The results indicated that Lactobacillus plantarum strain L15 had good potential for exerting antagonistic effects against E. coli.


Assuntos
Antibiose , Aderência Bacteriana , Células Epiteliais/microbiologia , Escherichia coli/fisiologia , Lactobacillus plantarum/fisiologia , Probióticos , Células CACO-2 , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Humanos , Lactobacillus plantarum/classificação , Lactobacillus plantarum/genética , Lactobacillus plantarum/isolamento & purificação , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
16.
Food Res Int ; 124: 109-117, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31466629

RESUMO

Tropical fruit and their industrial processing byproducts have been considered sources of probiotic Lactobacillus. Sixteen tropical fruit-derived Lactobacillus strains were assessed for growth-promoting effects using a host-commensal nutrient scarcity model with Drosophila melanogaster (Dm). Two Lactobacillus strains (L. plantarum 49 and L. plantarum 201) presenting the most significant effects (p ≤ .005) on Dm growth were selected and evaluated for their safety and beneficial effects in adult male Wistar rats during 28 days of administration of 9 log CFU/day, followed by 14 days of wash-out. Daily administration of L. plantarum 49 and L. plantarum 201 did not affect (p > .05) food intake or morphometric parameters. Both strains were associated with reduction (p ≤ .05) in blood glucose levels after 28 days of administration and after wash-out period; glucose levels remained reduced only in the group that received L. plantarum 49. Both strains were able to reduce (p ≤ .05) total cholesterol levels after 14 days of administration; after the wash-out period these levels remained reduced only in the group that received L. plantarum 201. L. plantarum 49 and L. plantarum 201 were detected in the intestine and did not cause alteration or translocate to spleen, kidneys or liver during the experimental or wash-out period. These results indicate that L. plantarum 49 and L. plantarum 201 present potential for use as probiotics with intrinsic abilities to modulate biochemical parameters of interest for the management of metabolic diseases.


Assuntos
Glicemia/efeitos dos fármacos , Colesterol/sangue , Frutas/microbiologia , Lactobacillus plantarum/fisiologia , Probióticos/farmacologia , Animais , Drosophila melanogaster , Fezes/microbiologia , Lactobacillus plantarum/isolamento & purificação , Fígado/microbiologia , Masculino , Ratos , Ratos Wistar , Baço/microbiologia
17.
Molecules ; 24(14)2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31319614

RESUMO

Kefir is a homemade, natural fermented product comprised of a probiotic bacteria and yeast complex. Kefir consumption has been associated with many advantageous properties to general health, including as an antioxidative, anti-obesity, anti-inflammatory, anti-microbial, and anti-tumor moiety. This beverage is commonly found and consumed by people in the United States of America, China, France, Brazil, and Japan. Recently, the consumption of kefir has been popularized in other countries including Malaysia. The microflora in kefir from different countries differs due to variations in culture conditions and the starter media. Thus, this study was aimed at isolating and characterizing the lactic acid bacteria that are predominant in Malaysian kefir grains via macroscopic examination and 16S ribosomal RNA gene sequencing. The results revealed that the Malaysian kefir grains are dominated by three different strains of Lactobacillus strains, which are Lactobacillus harbinensis, Lactobacillusparacasei, and Lactobacillus plantarum. The probiotic properties of these strains, such as acid and bile salt tolerances, adherence ability to the intestinal mucosa, antibiotic resistance, and hemolytic test, were subsequently conducted and extensively studied. The isolated Lactobacillus spp. from kefir H maintained its survival rate within 3 h of incubation at pH 3 and pH 4 at 98.0 ± 3.3% and 96.1 ± 1.7% of bacteria growth and exhibited the highest survival at bile salt condition at 0.3% and 0.5%. The same isolate also showed high adherence ability to intestinal cells at 96.3 ± 0.01%, has antibiotic resistance towards ampicillin, penicillin, and tetracycline, and showed no hemolytic activity. In addition, the results of antioxidant activity tests demonstrated that isolated Lactobacillus spp. from kefir G possessed high antioxidant activities for total phenolic content (TPC), total flavonoid content (TFC), ferric reducing ability of plasma (FRAP), and 1,1-diphenyl-2-picryl-hydrazine (DPPH) assay compared to other isolates. From these data, all Lactobacillus spp. isolated from Malaysian kefir serve as promising candidates for probiotics foods and beverage since they exhibit potential probiotic properties and antioxidant activities.


Assuntos
Antioxidantes/química , Kefir/microbiologia , Lactobacillus plantarum/isolamento & purificação , Lactobacillus/isolamento & purificação , Humanos , Lactobacillus/química , Lactobacillus/crescimento & desenvolvimento , Lactobacillus plantarum/química , Lactobacillus plantarum/crescimento & desenvolvimento , Malásia , Probióticos/química , Probióticos/isolamento & purificação
18.
Sci Rep ; 9(1): 9938, 2019 07 09.
Artigo em Inglês | MEDLINE | ID: mdl-31289291

RESUMO

We investigate the effects of postbiotic Lactobacillus plantarum RG14 on gastrointestinal histology, haematology, mucosal IgA concentration, microbial population and mRNA expression related to intestinal mucosal immunity and barrier function. Twelve newly weaned lambs were randomly allocated to two treatment groups; the control group without postbiotic supplementation and postbiotic group with supplementation of 0.9% postbiotic in the diet over a 60-day trial. The improvement of rumen papillae height and width were observed in lambs fed with postbiotics. In contrast, no difference was shown in villi height of duodenum, jejunum and ileum between the two groups. Lambs received postbiotics had a lower concentration of IgA in jejunum but no difference in IgA concentration in serum and mucosal of the rumen, duodenum and ileum. In respect of haematology, postbiotics lowered leukocyte, lymphocyte, basophil, neutrophil and platelets, no significant differences in eosinophil. The increase in of IL-6 mRNA and decrease of IL-1ß, IL-10, TNF mRNA were observed in the jejunum of lambs receiving postbiotics. Postbiotics also improved the integrity of the intestinal barrier by the upregulation of TJP-1, CLDN-1 and CLDN-4 mRNA. Postbiotic supplementation derived from L. plantarum RG14 in post-weaning lambs enhance the ruminal papillae growth, immune status and gastrointestinal health.


Assuntos
Ração Animal/microbiologia , Dieta/veterinária , Epitélio/efeitos dos fármacos , Mucosa Intestinal/efeitos dos fármacos , Lactobacillus plantarum/isolamento & purificação , Probióticos/farmacologia , Rúmen/efeitos dos fármacos , Animais , Epitélio/metabolismo , Epitélio/microbiologia , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Lactobacillus plantarum/metabolismo , Masculino , Rúmen/metabolismo , Rúmen/microbiologia , Ovinos , Desmame
19.
Food Chem ; 295: 361-367, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174770

RESUMO

This study was conducted to investigate the effects of Lactobacillus plantarum NJAU-01 isolated from Chinese dry-cured ham (Jinhua ham) on the regulation of protein oxidation of fermented sausages. Fermented sausages were prepared with different concentrations of L. plantarum NJAU-01 (105 CFU/g, 107 CFU/g and 109 CFU/g), and the commercial strain was used as positive control. The results showed that L. plantarum NJAU-01 at 107 CFU/g and 109 CFU/g significantly lowered the protein carbonyl content and protein surface hydrophobicity compared with the control (P < 0.05). The total sulfhydryl contents in L. plantarum NJAU-01 groups were significantly higher than that of the group without starter culture (P < 0.05). Significant changes were found in sarcoplasmic protein bands within 130-250 kDa, 100-130 kDa and 25-35 kDa during sausage fermentation (P < 0.05). Our data suggest that L. plantarum NJAU-01 has the potential to be an antioxidant starter culture in fermented sausages.


Assuntos
Alimentos e Bebidas Fermentados/microbiologia , Lactobacillus plantarum/fisiologia , Produtos da Carne/microbiologia , Proteínas de Carne/metabolismo , Animais , Antioxidantes/metabolismo , Contagem de Colônia Microbiana , Fermentação , Microbiologia de Alimentos , Lactobacillus plantarum/isolamento & purificação , Oxirredução , Carbonilação Proteica , Carne Vermelha/microbiologia , Suínos
20.
J Appl Microbiol ; 127(3): 778-793, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31211891

RESUMO

AIMS: Yeast and bacterial communities inhabit a sourdough starter to make artisanal bread. This study shows whether the interactions of micro-organisms derived from Australian sourdough starters provide some of the positive flavour, and aroma properties to bread by using defined sourdough cultures as the sole leaven in bread production. METHODS AND RESULTS: An investigation of Australian sourdough starters found that they contained Saccharomyces cerevisiae and Kazachstania exigua yeasts. When these yeasts were inoculated alone to ferment wheat flour in an extended fermentation, the bread had a heterogeneous crumb structure, a deeper colour and a distinctive chemical aroma profile than those made with commercial baker's yeast. When bread was made combining these yeasts individually and in combinations with lactic acid bacteria also isolated from these sourdough starters, including Lactobacillus plantarum, L. brevis, L. rossiae, L. casei, the bread aroma profiles and crumb structure were more distinctive, with compounds associated with sour aromas produced, and preferred by sensory panels. CONCLUSIONS: The use of defined mixed cultures as the leaven in bread making, by exploiting the microbial diversity of artisanal Australian starters, can produce bread with distinctive and attractive aromas. SIGNIFICANCE AND IMPACT OF THE STUDY: Understanding and identifying the community ecosystems found in sourdough cultures and using them as the sole leaven in bread production provide novel insights into microbial interactions and how they affect food quality by removing the effects of commercial yeast strains.


Assuntos
Pão/microbiologia , Técnicas de Cocultura , Tecnologia de Alimentos , Austrália , Fermentação , Farinha/microbiologia , Lactobacillales/isolamento & purificação , Lactobacillales/metabolismo , Lactobacillus plantarum/isolamento & purificação , Lactobacillus plantarum/metabolismo , Odorantes , Saccharomyces cerevisiae/isolamento & purificação , Saccharomyces cerevisiae/metabolismo , Saccharomycetales/isolamento & purificação , Saccharomycetales/metabolismo , Fermento Seco
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