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1.
Medicina (Kaunas) ; 57(6)2021 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-34071900

RESUMO

Background and Objectives: The purpose of the present study was to investigate the anti-bacterial efficacy of passive ultrasonic irrigation (PUI) and a 980-nm-gallium-aluminum-arsenide (GaAlAs) laser using a real-time DNA-based quantitative polymerase chain reaction (qPCR) assay and scanning electron microscopy (SEM). Materials and Methods: Eighty-six extracted single- and double-rooted human teeth were used in the experiment. The following four experimental groups were evaluated, as well as a control group: group 1: single root + PUI (n = 20); group 2: single root + laser application (n = 20); group 3: double roots + PUI (n = 20); group 4: double roots + laser application (n =20); control group (n = 6): 3 single roots, 3 double roots. The qPCR assay was performed in three stages to evaluate the efficacy of the adjunctive method against Enterococcus faecalis. SEM analysis was also used to examine the microstructure of root canal surfaces. The data were statistically analyzed using the Friedman test and the Kruskal-Wallis test with Bonferroni adjustment. Results: The decrease in the DNA levels from S1 (before preparation) to S2 (just after preparation) was highly significant in all groups, and decreases in DNA levels from S2 to S3 (after incubation for 1 week) were found in all experimental groups except group 1. An inter-group analysis showed that PUI was significantly more effective in terms of antibacterial efficacy than GaAlAs laser irradiation in single root (p < 0.05). However, in double roots, experimental groups did not show significantly lower DNA levels than the control group (p > 0.05). SEM images showed that cleaning of the root canal surface and reduction of dentin debris were achieved only in single-rooted teeth when using PUI application. Conclusions: Two adjunctive methods were effective in reducing E. faecalis in single rooted tooth.


Assuntos
Irrigantes do Canal Radicular , Hipoclorito de Sódio , Humanos , Lasers Semicondutores/uso terapêutico , Irrigação Terapêutica , Ultrassom
2.
Biomed Res Int ; 2021: 9984499, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33997052

RESUMO

Noncarious cervical lesions (NCCLs) are a common clinical finding often linked with dentin hypersensitivity (DH). Aim. The aim of the study was to evaluate the influence of diode laser for the treatment of DH on microleakage of subsequent NCCL restorations. Materials and Methods. Forty-eight extracted human premolars were collected. All teeth received standardized cervical preparation on both the buccal and palatal surfaces and were randomly divided into three groups (n = 16) according to the restorative material used: nanohybrid composite resin (CR), resin-modified glass ionomer (RMGI), and conventional glass ionomer (GIC). The prepared cavities on the palatal surfaces were treated by diode laser using SIROlaser Blue (Sirona Dental Systems, Bensheim, Germany) prior to restoration, while preparations on the buccal surfaces were directly restored. After thermocycling, the teeth were immersed in methylene blue dye for microleakage evaluation under 40x magnification at both occlusal and cervical margins. The Kruskal-Wallis test followed by the Bonferroni tests was conducted to determine inter- and intragroup differences (P < 0.05). Results. All restorative materials tested showed some degree of microleakage with no statistically significantly different scores with or without the use of laser desensitization prior to restorative treatment. Group CR showed the least microleakage, followed by group RMGI, while group GIC showed the highest. Cervical margins showed greater microleakage than the occlusal margins where the difference was statistically significant in the RMGI group without laser pretreatment (P = 0.006) and in both groups CR (P = 0.02) and RMGI (P = 0.006) with the laser pretreatment. Conclusion. Application of diode laser for the treatment of DH prior to the restoration of teeth with NCCL did not affect the microleakage of all the restorative materials tested. All the materials showed some degree of microleakage, which was higher in gingival margins compared to occlusal margins. The resin composite shows the least microleakage among all the tested materials.


Assuntos
Restauração Dentária Permanente/efeitos adversos , Sensibilidade da Dentina/terapia , Lasers Semicondutores , Resinas Acrílicas/química , Dente Pré-Molar/efeitos da radiação , Resinas Compostas/química , Humanos , Teste de Materiais , Modelos Biológicos , Dióxido de Silício/química
3.
Medicina (Kaunas) ; 57(5)2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33922785

RESUMO

Cesarean scar pregnancy (CSP) is a rare form of ectopic pregnancy which represents a consequence of a previous cesarean section. It is associated with major maternal morbidity and mortality and has potential implications on future fertility. Because of possible serious complications, CSP should be swiftly diagnosed and treated. There is no management protocol for this rare, life-threatening condition, and each patient should be evaluated individually. Several types of conservative treatment have been used to treat cesarean scar pregnancy: dilation and curettage (D&C), excision of trophoblastic tissues, local or systemic administration of methotrexate, bilateral hypogastric artery ligation, and selective uterine artery embolization with curettage and/or methotrexate administration. In our study we present a cesarean scar pregnancy of a 40-year-old woman who was treated with angiographic uterine artery embolization (UAE) followed by hysteroscopic diode laser resection. Our combined UAE-hysteroscopic laser surgery appears to offer an effective, safe, and minimally invasive surgical treatment.


Assuntos
Cesárea , Gravidez Ectópica , Adulto , Artérias , Cesárea/efeitos adversos , Cicatriz/complicações , Feminino , Humanos , Lasers Semicondutores , Metotrexato/uso terapêutico , Gravidez , Gravidez Ectópica/diagnóstico por imagem , Gravidez Ectópica/cirurgia , Doenças Raras , Estudos Retrospectivos , Resultado do Tratamento
4.
Oxid Med Cell Longev ; 2021: 6626286, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33763170

RESUMO

Photobiomodulation with 808 nm laser light electively stimulates Complexes III and IV of the mitochondrial respiratory chain, while Complexes I and II are not affected. At the wavelength of 1064 nm, Complexes I, III, and IV are excited, while Complex II and some mitochondrial matrix enzymes seem to be not receptive to photons at that wavelength. Complex IV was also activated by 633 nm. The mechanism of action of wavelengths in the range 900-1000 nm on mitochondria is less understood or not described. Oxidative stress from reactive oxygen species (ROS) generated by mitochondrial activity is an inescapable consequence of aerobic metabolism. The antioxidant enzyme system for ROS scavenging can keep them under control. However, alterations in mitochondrial activity can cause an increment of ROS production. ROS and ATP can play a role in cell death, cell proliferation, and cell cycle arrest. In our work, bovine liver isolated mitochondria were irradiated for 60 sec, in continuous wave mode with 980 nm and powers from 0.1 to 1.4 W (0.1 W increment at every step) to generate energies from 6 to 84 J, fluences from 7.7 to 107.7 J/cm2, power densities from 0.13 to 1.79 W/cm2, and spot size 0.78 cm2. The control was equal to 0 W. The activity of the mitochondria's complexes, Krebs cycle enzymes, ATP production, oxygen consumption, generation of ROS, and oxidative stress were detected. Lower powers (0.1-0.2 W) showed an inhibitory effect; those that were intermediate (0.3-0.7 W) did not display an effect, and the higher powers (0.8-1.1 W) induced an increment of ATP synthesis. Increasing the power (1.2-1.4 W) recovered the ATP production to the control level. The interaction occurred on Complexes III and IV, as well as ATP production and oxygen consumption. Results showed that 0.1 W uncoupled the respiratory chain and induced higher oxidative stress and drastic inhibition of ATP production. Conversely, 0.8 W kept mitochondria coupled and induced an increase of ATP production by increments of Complex III and IV activities. An augmentation of oxidative stress was also observed, probably as a consequence of the increased oxygen consumption and mitochondrial isolation experimental conditions. No effect was observed using 0.5 W, and no effect was observed on the enzymes of the Krebs cycle.


Assuntos
Lasers Semicondutores , Terapia com Luz de Baixa Intensidade , Mitocôndrias/metabolismo , Mitocôndrias/efeitos da radiação , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Animais , Bovinos , Respiração Celular/efeitos da radiação , Complexo III da Cadeia de Transporte de Elétrons/metabolismo , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Feminino , Isocitrato Desidrogenase/metabolismo , Peroxidação de Lipídeos/efeitos da radiação , Malato Desidrogenase/metabolismo , Masculino , Fosforilação Oxidativa/efeitos da radiação , Estresse Oxidativo/efeitos da radiação , ATPases Translocadoras de Prótons/metabolismo , Superóxidos/metabolismo , Temperatura
5.
J Equine Vet Sci ; 98: 103354, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33663730

RESUMO

Equine sinonasal myxomas (SNM) are very rare; only a few cases/small case series are reported in veterinary literature. The purpose of this report is to describe the diagnostic and surgical procedure adopted to approach the neoplastic mass in a case of equine SNM. A 5 year old, Murgese gelding was presented with mild serous nasal discharge, minimal facial swelling, decreased airflow from the right nostril, and dull frontal sinus percussion. Diagnostic imaging, including endoscopy, revealed a pale mass in the caudal portion of the right middle meatus, which developed inside the right conchofrontal sinus and nasal cavity and deviated the nasal septum to the left side. A large frontonasal bone flap was created with the horse in general anesthesia, aiming to remove the lesion and perform further diagnostic investigation. The mass had the shape of a small orange, the caudodorsal and rostral part having a hard wall, whereas the ventral part being friable; the inside was hollow, containing a viscous and transparent liquid. Surgical excision was broad but incomplete, and therefore after surgery, a standing transendoscopic diode laser irradiation and photoablation of small residual lesions per nasum were performed. Microscopically, the mass consisted of spindle-shaped cells, loosely arranged within an abundant, light blue myxoid matrix. On the basis of clinical and pathologic findings, the diagnosis of sinonasal myxoma was made. Although benign and slowly growing, myxomas usually tend to infiltrate the surrounding tissues; as a consequence, recurrence is very likely after surgical excision. In the case described herein, the combination of a surgical excision, transendoscopic diode laser irradiation, and photoablation provided a positive outcome, until at least 14 months after surgery.


Assuntos
Doenças dos Cavalos , Mixoma , Animais , Procedimentos Cirúrgicos de Citorredução/veterinária , Doenças dos Cavalos/cirurgia , Cavalos , Lasers Semicondutores/uso terapêutico , Masculino , Mixoma/diagnóstico por imagem , Mixoma/veterinária , Recidiva Local de Neoplasia/veterinária
6.
Anticancer Res ; 41(3): 1407-1420, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33788732

RESUMO

BACKGROUND/AIM: Recurrence and metastasis of cancer caused by cancer stem cells (CSCs) is a challenge to overcome. Low level laser therapy is a new treatment strategy to suppress their invasiveness. We have assessed the inhibitory effects of 470 nm blue LED on the invasiveness of them to determine the molecular mechanisms of anti-invasiveness. MATERIALS AND METHODS: The effects of blue LEDs on their viability, proliferation and invasion were analyzed using MTT and transwell methods. In addition, the anti-invasiveness effect of blue LED on them was evaluated by zymography, semi-quantitative RT-PCR and western blot analysis. RESULTS: Irradiation with blue LED at 3 J/cm2 resulted in inhibition of their viability, proliferation and invasiveness. Their matrix metalloproteinase 2 (MMP-2) and MMP-9 activities were reduced by blue LED irradiation. Semi-quantitative RT-PCR also showed similar results. In addition, western blotting analyses showed that cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) synthesis were significantly inhibited by LED irradiation in CD133+ colorectal CSCs. CONCLUSION: Down-regulation of the COX-2/PGE2 signaling pathway by blue LED irradiation led to reduce expression of MMP-2 and MMP-9, inhibiting the invasiveness of CD133+ colorectal CSC.


Assuntos
Antígeno AC133/metabolismo , Ciclo-Oxigenase 2/metabolismo , Dinoprostona/metabolismo , Lasers Semicondutores , Células-Tronco Neoplásicas/efeitos da radiação , Transdução de Sinais/efeitos da radiação , Antígeno AC133/genética , Proliferação de Células/genética , Proliferação de Células/efeitos da radiação , Sobrevivência Celular/genética , Sobrevivência Celular/efeitos da radiação , Neoplasias Colorretais/genética , Neoplasias Colorretais/metabolismo , Neoplasias Colorretais/patologia , Ciclo-Oxigenase 2/genética , Regulação para Baixo/efeitos da radiação , Regulação Neoplásica da Expressão Gênica/efeitos da radiação , Humanos , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/genética , Metaloproteinase 9 da Matriz/metabolismo , Invasividade Neoplásica , Células-Tronco Neoplásicas/metabolismo , Células Tumorais Cultivadas
7.
Am J Dent ; 34(1): 23-30, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33544985

RESUMO

PURPOSE: To investigate the effectiveness of different light activation methods for in-office bleaching agents in terms of color change durability and tooth sensitivity (TS). METHODS: 80 subjects were randomly divided into four groups (n= 20). A bleaching agent (40% H2O2) was activated using a neodymium:yttrium-aluminum-garnet (Nd:YAG) laser, an 810-nm diode laser, a light-emitting diode (LED), and conventional (chemical) treatment. The CIE L*a*b* system and ΔE* values were used to measure color changes in the teeth using a spectrophotometer. TS was measured using the visual analogue scale (VAS). The measurements were performed before the treatment, immediately afterwards, and 2 weeks, 1 month, and 6 months later. A P< 0.05 was accepted as statistically significant. RESULTS: The most statistically significant color change occurred in the 2nd week (⏊E2) in all groups (P< 0.05). However, the most color change in the 2nd week (ΔE2) occurred in the Nd:YAG group, yet there was no statistically significant result between Nd:YAG and LED (P< 0.05). After the procedure (VAS2), a statistically significant increase in TS was observed in all groups (P< 0.05), with the highest sensitivity observed in the LED group; significant differences were observed between the LED and the other groups (P< 0.05). CLINICAL SIGNIFICANCE: Although all light activation techniques showed noticeable color changes, the conventional technique showed appreciable color change at the 6-month follow-up. Nd:YAG and diode laser activation can be preferable alternatives to LED and conventional activation in clinical practice as they bleach more and faster, decrease the number of sessions and chair time, and cause minimum sensitivity.


Assuntos
Sensibilidade da Dentina , Clareadores Dentários , Clareamento Dental , Humanos , Peróxido de Hidrogênio , Lasers Semicondutores/uso terapêutico , Resultado do Tratamento
8.
BMC Oral Health ; 21(1): 80, 2021 02 19.
Artigo em Inglês | MEDLINE | ID: mdl-33607998

RESUMO

BACKGROUND: Periodontitis is a chronic inflammatory disease characterized by progressive damage on the structure of tooth-supporting tissues. The aim of the study is determining the combination photodynamic effect of diode laser 405 nm treatments and the administration of doxycycline 0.1% within 1, 3, 5, and 7 days on a Wistar rat model of periodontitis. METHODS: Samples were induced with Porphyromonas gingivalis ATCC33277 to allow periodontitis development and were treated with combination of doxycycline and laser diode, then statistical analysis was carried out (One-Way ANOVA test and the post-hoc Duncan test; Kruskal-Wallis test and Mann-Whitney follow-up test for non-parametric data). Samples were divided into five groups, laser exposure used was 405-nm diode laser with energy density of 8 J/cm2. The expression level of histomorphometric was calculated by measuring the number of macrophages, lymphocytes, fibroblasts and the distance between the CEJ-AV. RESULTS: The results showed that the combination treatment of doxycycline and laser exposure yielded immunomodulatory effects. The expression level of fibroblast and the distance between CEJ-AV bone showed that the combination of doxycycline and laser therapy exerted healing effect in rat models of periodontitis on day 5 and 7. CONCLUSION: The combination of doxycycline 0.1% and diode laser therapy provides a healing effect in rats models of periodontitis.


Assuntos
Periodontite , Fotoquimioterapia , Animais , Doxiciclina/uso terapêutico , Lasers Semicondutores/uso terapêutico , Periodontite/tratamento farmacológico , Ratos , Ratos Wistar
9.
J Cosmet Dermatol ; 20(4): 1117-1123, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33567152

RESUMO

BACKGROUND: Laser hair reduction is one of the most common laser procedures in dermatology. Laser systems available are Ruby at 694 nm, Alexandrite (755 nm), Diode (810nm), and Nd:YAG (1064 nm). Recently, devices presenting a combination of wavelengths have shown to produce high effective hair reduction with no related risks. This study was conducted with the Soprano Ice Platinum (Alma Lasers, GMBH, Germany) that emits a combination of three wavelengths at 755, 810, and 1064 nm in a single pulse. AIMS: To establish the efficacy and safety of novel triple wavelength laser hair reduction technology in skin types IV to V. PATIENTS AND METHODS: This was an investigator initiated, single-center, prospective clinical study conducted between December 2017 and June 2019 with a total of 25 patients (23 female and two male) above 18 years with skin types IV and V, areas included are face, beard, neck, chest, and others. RESULTS: Hair reduction was assessed using the physician GAIS (global esthetic improvement scale) score: no results 0% reduction, poor 0%-25% reduction, average 25%-50%, good 50%-75%, and excellent 75%-100%. All 25 patients (mean age 28 years, range 18-52 y/o) completed all six sessions of the study. CONCLUSION: Objective clinical assessment of hair reduction using the physician GAIS score in 90 anatomical sites scores an excellent reduction in 95.5% areas and good reduction in 80% areas. This current study shows that a synergistic combination of three diode laser wavelengths used for hair removal (755, 810, 1064 nm) is effective and safe to use in subjects skin types IV and V for facial and body hair.


Assuntos
Remoção de Cabelo , Terapia a Laser , Adolescente , Adulto , Feminino , Cabelo , Remoção de Cabelo/efeitos adversos , Humanos , Lasers Semicondutores/efeitos adversos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Resultado do Tratamento , Adulto Jovem
10.
Spectrochim Acta A Mol Biomol Spectrosc ; 253: 119563, 2021 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-33621933

RESUMO

Laser-based infrared (IR) spectroscopy is an emerging key technology for the analysis of solutes and for real-time reaction monitoring in liquids. Larger applicable pathlengths compared to the traditional gold standard Fourier transform IR (FTIR) spectroscopy enable robust measurements of analytes in a strongly absorbing matrix such as water. Recent advancements in laser development also provide large accessible spectral coverage thus overcoming an inherent drawback of laser-based IR spectroscopy. In this work, we benchmark a commercial room temperature operated broadband external cavity-quantum cascade laser (EC-QCL)-IR spectrometer with a spectral coverage of 400 cm-1 against FTIR spectroscopy and showcase its application for measuring the secondary structure of proteins in water, and for monitoring the lipase-catalyzed saponification of triacetin. Regarding the obtained limit of detection (LOD), the laser-based spectrometer compared well to a research-grade FTIR spectrometer employing a liquid nitrogen cooled detector. With respect to a routine FTIR spectrometer equipped with a room temperature operated pyroelectric detector, a 15-fold increase in LOD was obtained in the spectral range of 1600-1700 cm-1. Characteristic spectral features in the amide I and amide II region of three representative proteins with different secondary structures could be measured at concentrations as low as 0.25 mg mL-1. Enzymatic hydrolysis of triacetin by lipase was monitored, demonstrating the advantage of a broad spectral coverage for following complex chemical reactions. The obtained results in combination with the portability and small footprint of the employed spectrometer opens a wide range of future applications in protein analysis and industrial process control, which cannot be readily met by FTIR spectroscopy without recurring to liquid nitrogen cooled detectors.


Assuntos
Lasers Semicondutores , Proteínas , Estrutura Secundária de Proteína , Espectrofotometria Infravermelho , Espectroscopia de Infravermelho com Transformada de Fourier
11.
J Plast Reconstr Aesthet Surg ; 74(5): 1050-1060, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33436333

RESUMO

Light energy is harnessed for therapeutic use in a number of ways, most recently by way of photobiomodulation (PBM). This phenomenon is a cascade of physiological events induced by the nonthermal exposure of tissue to light at the near infrared end of the visible spectrum. Therapeutic PBM has become a highly commercialized interest, marketed for everything from facial rejuvenation to fat loss, and diode-based devices are popular in both the clinic setting and for use at home. The lack of regulatory standards makes it difficult to draw clear conclusions about efficacy and safety but it is crucial that we understand the theoretical basis for PBM, so that we can engage in an honest dialogue with our patients and design better clinical studies to put claims of efficacy to the test. This article presents a summary of the science of PBM and examines the differences between laser light, on which much of the preclinical evidence is based and light from diodes, which are typically used in a clinical setting.


Assuntos
Lasers Semicondutores/uso terapêutico , Terapia com Luz de Baixa Intensidade/métodos , Rejuvenescimento , Animais , Proliferação de Células/efeitos da radiação , Relação Dose-Resposta à Radiação , Humanos , Células-Tronco Mesenquimais/efeitos da radiação , Estresse Oxidativo
12.
Acta Cir Bras ; 35(12): e351204, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33503217

RESUMO

PURPOSE: To evaluate the effect of diode laser use on experimental orthodontic tooth movements. METHODS: Thirty Rattus norvegicus albinus Wistar were divided into three equal groups (n = 10), two experimentals and one control. Applying 20 g orthodontic force were attached to the maxillary incisors of the rats in all groups. Low dose laser was applied to the surrounding tissues of the maxillary incisors of the rats in the experimental groups. Two exposure times for laser irradiation were used for seven days: t = 12 min (energy dose = 72 J) and t = 9 min (energy dose = 54 J) by a 0.1 W DEKA brand diode laser with wavelength of 980 nm. RESULTS: Osteoclastic activation increased in the 72 J group when compared to control group and decreased in comparison to the 54 J group. Osteoblastic activation was decreased in the 72 J group when compared to the control group and increased in comparison to the 54 J group. CONCLUSIONS: Applying 54 J laser energy has been found effective to accelerate the orthodontic tooth movement.


Assuntos
Terapia com Luz de Baixa Intensidade , Técnicas de Movimentação Dentária , Animais , Lasers Semicondutores , Osteoclastos , Ratos , Ratos Wistar
13.
BMC Oral Health ; 21(1): 41, 2021 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-33482797

RESUMO

BACKGROUND: This study aimed to assess the effect of a 980-nm diode laser following chemomechanical root canal preparation on the severity of post-operative pain (PP) after root canal treatment (RCT). METHODS: This study included asymptomatic, single-rooted teeth with a periapical index (PAI) score of 3 or 4. All the patients received RCT, including dressing with calcium hydroxide (Ca(OH)2), during two visits. The patients were randomly divided into two groups (n:30): a control group, in which the final irrigation was performed using 5 ml of 2.5% sodium hypochlorite (NaOCI), followed by 5 ml of 17% and ethylenediaminetetraacetic acid (EDTA) and 5 ml of distilled water, and a laser group, in which the root canals were irradiated using a 980-nm diode laser after the final irrigation at both visits. The pain levels after both visits were evaluated using a visual analogue scale (VAS) after 8 h, 24 h, 48 h and 7 d. In addition, the time intervals to medication intake were recorded. The collected data were statistically analysed using the chi-square and Mann-Whitney U test (p < 0.05). RESULTS: The average pain level in the control group 24 h after the first visit was significantly higher than that in the laser group (p < 0.05). The average pain level 24 h and 48 h after the second visit was significantly higher in the control group (p < 0.05). The levels of PP 24 h after the first visit were higher than those after the second visit only in the control group (p < 0.05). After the first visit, analgesic use in the control group was significantly higher after 8 h (40%) and 24 h (23%) as compared with that in the laser group (p < 0.05). CONCLUSIONS: Root canal irradiation with a diode laser may reduce PP after RCT in single-rooted teeth with a PAI score of 3 or 4. TRIAL REGISTRATION: Effect of the Diode Laser on Post-operative Pain After Endodontic Treatment in Teeth with Apical Periodontitis: NCT04486196. Registered 24 July 2020-Retrospectively registered, http://clinicaltrials.gov/ct2/show/NCT04486196.


Assuntos
Lasers Semicondutores , Periodontite Periapical , Cavidade Pulpar , Humanos , Lasers Semicondutores/uso terapêutico , Dor Pós-Operatória , Periodontite Periapical/cirurgia , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Tratamento do Canal Radicular/efeitos adversos , Hipoclorito de Sódio/uso terapêutico
14.
Clin Oral Investig ; 25(5): 2737-2744, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33409689

RESUMO

OBJECTIVES: This study aimed to investigate the ability of the diode and Nd:YAG lasers to decrease postoperative pain/discomfort after intracanal application in vital and devital endodontic cases. MATERIAL AND METHODS: One hundred two patients with symptomatic irreversible pulpitis and asymptomatic necrotic pulps were selected and randomly divided into three groups. After chemomechanical preparation, Nd:YAG and diode laser was applied to first and second groups for final treatment of the root canals. In the control group, no further disinfection protocol was performed upon traditional procedures. Then all the teeth were obturated in the same appointment. Pre- and postoperative pain were recorded using the VAS at 0, 12th, 24th, 48th, and 72nd hours following the treatment Kruskal-Wallis and Dunn test. RESULTS: VAS scores were significantly the most at 12-h period compared with other time periods after treatment for all groups. There was no significant difference among Nd:YAG laser, diode laser, and control groups in non-vital cases (p > 0.05). In vital cases, Nd:YAG laser group represented significantly fewer scores compared with diode laser and control groups at 48-h period (p < 0.05). CONCLUSIONS: The processes involving the use of Nd:YAG and diode lasers after chemomechanical preparation was found to be equivalent in terms of postoperative pain intensity after completion of treatment in one session both in vital or non-vital cases except in vital cases in Nd:YAG group at 48 h. CLINICAL RELEVANCE: Adjunct clinical use of Nd:YAG or diode lasers to traditional chemomechanical preparation did not cause any improvement or deterioration on postoperative discomfort and pain in patients with symptomatic irreversible pulpitis and asymptomatic necrotic pulps.


Assuntos
Lasers de Estado Sólido , Pulpite , Necrose da Polpa Dentária/terapia , Humanos , Lasers Semicondutores/uso terapêutico , Lasers de Estado Sólido/uso terapêutico , Dor Pós-Operatória , Pulpite/cirurgia
15.
Spectrochim Acta A Mol Biomol Spectrosc ; 251: 119411, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33450450

RESUMO

In this contribution we review selected point-of-care applications of infrared spectroscopy and the technological innovations they are based on. After a short introduction summarizing the general idea behind point-of-care applications we introduce the reader to important infrared spectroscopy sensing principles on a very basic level. We discuss the role of optical components like quantum cascade lasers, supercontinuum sources, waveguides and how they are potentially going to revolutionize point-of-care applications. First, we focus on the technological solutions of some principal problems like increasing the pathlength in a transmission cell to enhance the sensitivity for solutes in aqueous solutions and discuss indirect methods which circumvent the problem of low transmittance. In the second part we show how the technological progress of the last decades enabled scientific progress leading to selected concrete and outstanding point-of-care solutions and applications based on infrared spectroscopy. These include the detection and quantification of malaria parasitemia, early recognition of Alzheimer's disease long before the onset of clinical symptoms and a non-invasive method for testing the blood glucose content. The selected examples demonstrate and showcase that infrared spectroscopy is on the way to become an indispensable technique for point-of-care applications.


Assuntos
Malária , Sistemas Automatizados de Assistência Junto ao Leito , Humanos , Lasers Semicondutores , Espectrofotometria Infravermelho
16.
Quintessence Int ; 52(2): 140-153, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33433080

RESUMO

OBJECTIVE: The present systematic review aimed to assess the efficacy of photobiomodulation (PBM) therapy on neurosensory recovery of patients with inferior alveolar nerve injury following third molar surgery or dental implant placement. METHOD AND MATERIALS: An electronic search was carried out in Scopus, Embase, Medline, PubMed, Web of Science, Cochrane Library, and Google Scholar databases. Among 1,122 identified papers, seven articles (three RCTs, one observational study, and three case series) met the inclusion criteria.
Results: Time lapse from nerve injury to the onset of PBM therapy varied widely from 2 days to 4 years. The number of patients in each study ranged between 4 and 74. In the majority of the studies, PBM was done using a diode laser at wavelengths ?in the range of 808 to 830 nm with power of 5 to 500 mW and radiation dose of 3 to 244 J/cm2. Two out of three RCTs found significant neurosensory recovery in the patients who received PBM therapy compared to the controls. The observational study and all case series reported significant improvement in the neurosensory status following PBM therapy. The degree of neurosensory recovery was found to be greater in younger patients and those who received the treatment within 6 months following the injury.
Conclusions: Due to the limited number of well-designed RCTs and small number of patients in each study, it is not possible to make a clear conclusion about the efficacy of PBM therapy on neurosensory recovery in patients with inferior alveolar nerve injury following third molar or implant procedures. Considering the possibility of spontaneous inferior alveolar nerve recovery during this period, the conclusion based on the studies with no control group should be interpreted with caution. (Quintessence Int 2021;52:140-153; doi: 10.3290/j.qi.a45430).


Assuntos
Terapia com Luz de Baixa Intensidade , Humanos , Lasers Semicondutores , Nervo Mandibular
17.
J Nanosci Nanotechnol ; 21(2): 1323-1330, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33183479

RESUMO

With the development of nanometer semiconductor laser technology, due to the wide range of photobiological regulation and non-invasive advantages, it is widely used in clinical research, including reducing pain, accelerating wound healing, nerve injury repair and regeneration. Increase tissue blood flow, improve anxiety and depression, and treat Parkinson's and retinal diseases. However, in many studies, the role of photobiological regulation is still controversial. There are two main problems, one is that the mechanism of photo biological regulation is not fully understood, and the other is that the specific parameters are not uniform in different treatments, such as wavelength density, power density, pulse, treatment timing, and number of treatments. In this paper, through the second question, the parameters of low-energy near-infrared light (810 nm semiconductor laser) in the treatment of fundus diseases are the main research objects. Based on understanding the parameters of low-energy lasers, cyan blue is irradiated with different energy near-infrared light. Data analysis of the actual energy obtained after the retina of the rabbit and observation and research on the cell morphology of each layer of the retina, to obtain relatively safe treatment parameters for the retina, provide theoretical data for near-infrared light in the treatment of clinical fundus disease, and make it safer to use in clinical treatment.


Assuntos
Anti-Hipertensivos , Lasers Semicondutores , Animais , Raios Infravermelhos , Lasers Semicondutores/uso terapêutico , Coelhos , Tecnologia
18.
J Oral Maxillofac Surg ; 79(3): 685-693, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33358708

RESUMO

PURPOSE: Evidence shows that low-level laser therapy (LLLT) may shorten the recovery time of healing wounds. This study sought to assess the effect of LLLT on the recovery time of neurosensory deficits after Le Fort I osteotomy and bilateral sagittal split osteotomy using the blink reflex. METHODS: This randomized double-blind clinical trial evaluated 12 patients undergoing orthognathic surgery of the maxilla/mandible. One random quadrant of the maxilla/mandible underwent 810 nm gallium-aluminum-arsenide laser (infrared spectrum, 200 mW, 12 J/cm2, 60-second irradiation time of each point, continuous-wave mode), while the laser handpiece was used in off mode for the other quadrant, postoperatively. The patients underwent LLLT at 1, 5, 10, and 14 days, postoperatively. The blink test was performed to assess the function of the trigeminal nerve, and the 2-point discrimination (TPD) test was performed to assess the recovery of the inferior alveolar nerve (IAN) and the infraorbital nerves at 30 and 90 days, postoperatively. Data were analyzed using the Mann-Whitney U test (alpha = 0.05). RESULTS: Six women and 6 men were evaluated (mean age = 23.41 ± 5.03 years). Both groups showed significant improvement in function of the IAN and infraorbital nerves, postoperatively in blink reflex test (P < .05). In the TPD test, although both groups showed significant improvement of the IAN compared with the baseline (P < .05), the IAN showed significantly greater improvement by 1.1% in the laser group (P = .036). The percentage of change was significantly smaller in the laser group in R1 (by 14%; P = .025) and R2C wavelengths of the IAN and in the TPD test of the IAN (by 20%; P = .021), showing significantly greater improvement in laser group. CONCLUSIONS: LLLT with 810-nm diode laser can accelerate the recovery from paresthesia after orthognathic surgery. The blink reflex can be used as a quantitative index for neurosensory assessment in line with subjective clinical tests such as the TPD test.


Assuntos
Terapia com Luz de Baixa Intensidade , Cirurgia Ortognática , Procedimentos Cirúrgicos Ortognáticos , Traumatismos do Nervo Trigêmeo , Adolescente , Adulto , Piscadela , Feminino , Humanos , Lasers Semicondutores , Masculino , Mandíbula , Nervo Mandibular , Adulto Jovem
19.
Am J Otolaryngol ; 42(1): 102778, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33137675

RESUMO

OBJECTIVES: The aim of this study was to compare radiofrequency ablation (RFA), diode laser, and microdebrider-assisted inferior turbinoplasty (MAIT) in the treatment of chronic nasal obstruction in a one-year follow-up, and to pay special attention to the effect of the procedures on the contractility capacity of the inferior turbinates. METHODS: The patients filled a Visual Analogue Scale (VAS) questionnaire regarding nasal symptoms pre- and postoperatively. Saccharin transit time (STT) evaluation and acoustic rhinometry were also performed. A total of 77 patients attended the one-year control visit and had technically reliable acoustic rhinometry results. RESULTS: All the examined techniques decreased the VAS score for the severity of nasal obstruction statistically significantly. There was no deterioration found in the symptoms of crusting, nasal discharge, and sneezing, nor in mucociliary function in any of the groups. All the three techniques increased the non-decongested total V2-5 cm values and decreased the decongested total V2-5 cm values statistically significantly. The V2-5 cm change (%) values decreased statistically significantly in the RFA, diode laser, and the MAIT groups following the operations, the mean changes being -57 percentage points (pp), -53 pp, and -73 pp respectively. CONCLUSION: All three techniques decreased the severity of nasal obstruction significantly in the one-year follow-up. Although submucosal fibrosis seemed to increase, all the techniques increased the anterior nasal cavity volume significantly. Inferior turbinate contractility decreased to the normal level from the preoperative congested state following the surgery with every examined technique.


Assuntos
Desbridamento/métodos , Lasers Semicondutores , Obstrução Nasal/cirurgia , Procedimentos Cirúrgicos Nasais/métodos , Ablação por Radiofrequência/métodos , Microcirurgia Endoscópica Transanal/métodos , Conchas Nasais/cirurgia , Adulto , Doença Crônica , Feminino , Seguimentos , Humanos , Masculino , Estudos Prospectivos , Resultado do Tratamento , Conchas Nasais/fisiopatologia
20.
ACS Sens ; 6(1): 35-42, 2021 01 22.
Artigo em Inglês | MEDLINE | ID: mdl-33372759

RESUMO

In this work, we introduce polarimetric balanced detection as a new attenuated total reflection (ATR) infrared (IR) sensing scheme, leveraging unequal effective thicknesses achieved with laser light of different polarizations. We combined a monolithic widely tunable Vernier quantum cascade laser (QCL-XT) and a multibounce ATR IR spectroscopy setup for analysis of liquids in a process analytical setting. Polarimetric balanced detection enables simultaneous recording of background and sample spectra, significantly reducing long-term drifts. The root-mean-square noise could be improved by a factor of 10 in a long-term experiment, compared to conventional absorbance measurements obtained via the single-ended optical channel. The sensing performance of the device was further evaluated by on-site measurements of ethanol in water, leading to an improved limit of detection (LOD) achieved with polarimetric balanced detection. Sequential injection analysis was employed for automated injection of samples into a custom-built ATR flow cell mounted above a zinc sulfide multibounce ATR element. The QCL-XT posed to be suitable for mid-IR-based sensing in liquids due to its wide tunability. Polarimetric balanced detection proved to enhance the robustness and long-term stability of the sensing device, along with improving the LOD by a factor of 5. This demonstrates the potential for new polarimetric QCL-based ATR mid-IR sensing schemes for in-field measurements or process monitoring usually prone to a multitude of interferences.


Assuntos
Lasers Semicondutores , Água , Espectrofotometria Infravermelho
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