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1.
Medicine (Baltimore) ; 98(39): e17319, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31574863

RESUMO

BACKGROUND: Heroin addiction remains a significant public health problem worldwide, and relapse to heroin use following cessation of agonist maintenance treatment is common. The problems associated with use of opioid agonists mean that non-opioid therapies need to be developed to ameliorate acute and protracted opioid withdrawal syndromes. METHODS: Fifteen men with opioid use disorder on methadone maintenance treatment have been enrolled from an addiction treatment center as an experimental group in this case-controlled study. This group is receiving laser meridian massage on the back, including the Bladder meridian and Governor Vessel, 3 times weekly for 4 weeks. An age-matched control group that does not receive laser meridian massage has also been enrolled. Urinary morphine levels are being checked before and after 2 and 4 weeks of treatment. Subjects are requested to self-report their number of episodes or days of heroin use and 0 to 10-point visual analogue scale scores for heroin craving/refusal to use heroin during the previous week before and after 2 and 4 weeks of treatment. Quality of life will be reported using the Short Form-12v2 before and after 4 weeks of treatment. Pulse diagnosis will be recorded and heart rate variability calculated after one single treatment session. The baseline patient characteristics will be compared between the experimental and control groups using the independent t test and Chi-square test. Data are compared between the 2 groups using repeated-measures analysis of variance, generalized estimating equations, and the paired t test. OBJECTIVE: To investigate the effect of adjuvant laser meridian massage in men with opioid use disorder on methadone maintenance treatment. TRIAL REGISTRATION: ClinicalTrials.gov NCT04003077.


Assuntos
Lasers , Massagem , Meridianos , Metadona/uso terapêutico , Tratamento de Substituição de Opiáceos/métodos , Transtornos Relacionados ao Uso de Opioides/terapia , Pontos de Acupuntura , Adulto , Analgésicos Opioides/uso terapêutico , Estudos de Casos e Controles , Humanos , Masculino , Massagem/instrumentação , Massagem/métodos , Síndrome de Abstinência a Substâncias/terapia
2.
Coluna/Columna ; 18(3): 176-180, July-Sept. 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1019777

RESUMO

ABSTRACT Objective To evaluate the effects of photodynamic therapy (PDT) on the dura mater using the photosensitizers aluminum chloride phthalocyanine and methylene blue in in vivo assays. Methods Fifty-six male Wistar rats were divided into two groups; one submitted to PDT and the other submitted to the photosensitizers without their photoactivation (control). The photosensitizers were applied to the dura mater after laminectomy at the T10 level. The methods used for assessment were the Basso, Beattie and Bresnahan (BBB) functional evaluation scale and study of the dura mater by light microscopy. Results No changes in motor activity were observed in the animals submitted to PDT compared to control. Histological and pathological evaluation did not show any differences between the group exposed to activated photosensitizers and the control group with regard to the inflammatory process and tissue necrosis. Conclusion The joint use of PDT with the photosensitizing pharmaceuticals aluminum chloride phthalocyanine and methylene blue did not induce any clinical neurotoxic effects or histological changes in the dura mater of the animals studied. Level de evidence V; Expert Opnion.


RESUMO Objetivo Avaliar os efeitos da terapia fotodinâmica (PDT) na dura-máter usando os fotossensibilizadores cloreto de alumínio ftalocianina e azul de metileno em ensaios in vivo. Métodos Cinquenta e seis ratos Wistar machos divididos em dois grupos; um submetido à PDT e o outro submetido aos fotossensibilizadores sem a fotoativação (controle). Os fotossensibilizadores foram aplicados sobre a dura-máter depois de laminectomia no nível T10. Os métodos de avaliação usados foram a escala de avaliação funcional de Basso, Beattie e Bresnahan (BBB) e o estudo da dura-máter por microscopia óptica. Resultados Não foram observadas alterações da atividade motora dos animais submetidos à PDT com relação ao grupo controle. A avaliação histológica e histopatológica não mostrou diferenças entre o grupo exposto aos fotossensibilizadores ativados e o grupo controle, com relação ao processo inflamatório e à necrose tecidual. Conclusões O uso conjunto de PDT e os fármacos fotossensibilizantes cloreto de alumínio ftalocianina e azul de metileno não induziu efeitos neurotóxicos clínicos e/ou alterações histológicas sobre a dura-máter dos animais estudados. Nível de evidência V; Opinião de Especialista.


RESUMEN Objetivo Evaluar los efectos de la terapia fotodinámica (PDT) en la duramadre utilizando los fotosensibilizadores de ftalocianina de aluminio clorada y azul de metileno en ensayos in vivo. Métodos Cincuenta y seis ratas Wistar machos se dividieron en 2 grupos; uno fue sometido a PDT y el otro sometido a fotosensibilizadores sin fotoactivación (control). Los fotosensibilizadores se aplicaron a la duramadre después de la laminectomía en el nivel T10. Los métodos de evaluación utilizados fueron la escala de evaluación funcional de Basso, Beattie y Bresnahan (BBB) y el estudio de la duramadre mediante microscopía óptica. Resultados No hubo cambios en la actividad motora de los animales sometidos a PDT en relación con el grupo de control. La evaluación histológica e histopatológica no mostró diferencias entre el grupo expuesto a fotosensibilizadores activados y el grupo de control con respecto al proceso inflamatorio y la necrosis tisular. Conclusiones El uso conjunto de PDT con las sustancias fotosensibilizadores ftalocianina de aluminio clorada y azul de metileno no indujo efectos neurotóxicos clínicos o cambios histológicos en la duramadre de los animales estudiados. Nivel de evidencia V; Opinión del Especialista.


Assuntos
Humanos , Dura-Máter , Compostos Fitoquímicos , Lasers , Azul de Metileno
3.
Klin Lab Diagn ; 64(8): 503-506, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31479608

RESUMO

The results of the bacteriological catheter investigation on the analyzer with the technology of laser light scattering and using D. Maki culture technique coincided in 91.8% of cases. Catheter-associated infections are proven in 8 cases. The following obtained from blood and intravascular catheter in 5 patients: Staphylococcus epidermidis (n=1), Staphylococcus haemolyticus (n=1), Escherichia coli (n=1), Staphylococcus aureus (n=2); Staphylococcus epidermidis (n=1) was detected in 1 child from ventricular shunt and cerebrospinal fluid and Escherichia coli lactose-negative + Staphylococcus haemolyticus were detected in 1 child from ventricular shunt, whily only Escherichia coli lactose-negative was detected from cerebrospinal fluid; Pseudomonas aeruginosa (n=1) was foud out in 1 patient from the urinary catheter and urine. Clinical significance of the isolated microorganisms from the catheter must be assessed in each particular case taking into account its quantity and type of the isolated microorganism.


Assuntos
Infecções Bacterianas/diagnóstico , Infecções Relacionadas a Cateter/diagnóstico , Lasers , Criança , Escherichia coli , Humanos , Pseudomonas aeruginosa , Staphylococcus aureus , Staphylococcus epidermidis , Staphylococcus haemolyticus
4.
Vestn Oftalmol ; 135(3): 128-136, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31393457

RESUMO

Femtosecond laser-assisted cataract surgery (FLACS) is a new chapter in the history of cataract surgery. The use of femtosecond laser allows automation of the main stages of operation such as capsulotomy, lens fragmentation and creation of corneal incisions; it improves predictability and safety of the procedure and improves refractive outcomes. This article analyzes advantages and disadvantages of the new technology based on literature data and authors' own experience.


Assuntos
Extração de Catarata , Catarata , Terapia a Laser , Cristalino , Facoemulsificação , Humanos , Lasers
5.
J Chem Theory Comput ; 15(8): 4526-4534, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31314524

RESUMO

We investigate the photoinduced ultrafast charge migration phenomenon in the glycine molecule using a recently proposed nonequilibrium Green's functions (NEGF) approach. We first consider the dynamics resulting from the sudden removal of an electron in the valence shells, finding a satisfactory agreement with available data. Then we explicitly simulate the laser-induced photoionization process and study the evolution of the system after the pulse. We disentangle polarization and correlation effects in the electron dynamics and assign the main frequencies to specific elements of the reduced one-particle density matrix. We show that electronic correlations renormalize the bare frequencies, redistribute the spectral weights, and give rise to new spectral features.


Assuntos
Elétrons , Glicina/química , Íons/química , Lasers , Modelos Moleculares , Processos Fotoquímicos
6.
J Nanobiotechnology ; 17(1): 83, 2019 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-31291948

RESUMO

BACKGROUND: Macrophages with tumor-tropic migratory properties can serve as a cellular carrier to enhance the efficacy of anti neoplastic agents. However, limited drug loading (DL) and insufficient drug release at the tumor site remain the main obstacles in developing macrophage-based delivery systems. In this study, we constructed a biomimetic delivery system (BDS) by loading doxorubicin (DOX)-loaded reduced graphene oxide (rGO) into a mouse macrophage-like cell line (RAW264.7), hoping that the newly constructed BDS could perfectly combine the tumor-tropic ability of macrophages and the photothermal property of rGO. RESULTS: At the same DOX concentration, the macrophages could absorb more DOX/PEG-BPEI-rGO than free DOX. The tumor-tropic capacity of RAW264.7 cells towards RM-1 mouse prostate cancer cells did not undergo significant change after drug loading in vitro and in vivo. PEG-BPEI-rGO encapsulated in the macrophages could effectively convert the absorbed near-infrared light into heat energy, causing rapid release of DOX. The BDS showed excellent anti-tumor efficacy in vivo. CONCLUSIONS: The BDS that we developed in this study had the following characteristic features: active targeting of tumor cells, stimuli-release triggered by near-infrared laser (NIR), and effective combination of chemotherapy and photothermotherapy. Using the photothermal effect produced by PEG-BPEI-rGO and DOX released from the macrophages upon NIR irradiation, MAs-DOX/PEG-BPEI-rGO exhibited a significant inhibitory effect on tumor growth.


Assuntos
Antineoplásicos/química , Materiais Biomiméticos/química , Portadores de Fármacos/química , Macrófagos/metabolismo , Neoplasias da Próstata/tratamento farmacológico , Animais , Antineoplásicos/administração & dosagem , Linhagem Celular Tumoral , Doxorrubicina/administração & dosagem , Doxorrubicina/química , Liberação Controlada de Fármacos , Grafite/química , Humanos , Hipertermia Induzida , Raios Infravermelhos , Lasers , Masculino , Camundongos Endogâmicos BALB C , Polietilenoglicóis/química , Polietilenoimina/análogos & derivados , Polietilenoimina/química , Distribuição Tecidual
7.
J Laryngol Otol ; 133(8): 700-703, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31317846

RESUMO

OBJECTIVE: The completion of a laser safety course remains a core surgical curriculum requirement for otolaryngologists training in the UK. This project aimed to develop a comprehensive laser safety course utilising both technical and non-technical skills simulation. METHODS: Otolaryngology trainees and consultants from the West of Scotland Deanery attended a 1-day course comprising lectures, two high-fidelity simulation scenarios and a technical simulation of safe laser use in practice. RESULTS: The course, and in particular the use of simulation training, received excellent feedback from otolaryngology trainees and consultants who participated. Both simulation scenarios were validated for future use in laser simulation. CONCLUSION: The course has been recognised as a laser safety course sufficient for the otolaryngology Certificate of Completion of Training. To the authors' knowledge, this article represents the first description of using in situ non-technical skills simulation training for teaching laser use in otolaryngology.


Assuntos
Lasers/efeitos adversos , Otolaringologia/educação , Treinamento por Simulação/métodos , Competência Clínica , Simulação por Computador , Educação Médica Continuada , Humanos , Internato e Residência , Reino Unido
8.
Sci Total Environ ; 691: 205-215, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31319256

RESUMO

Forest stands are often parameterized by vegetation indices such as the Leaf Area Index (LAI). However, other indices (i.e. stand denseness, espacement, canopy density, canopy cover, foliage cover, crown porosity, gap fraction) may better characterize forest structure. Terrestrial and airborne active sensor data has been used to describe canopy structural diversity and provide accurate estimates of forest structure indices. This study uses Terrestrial Laser Scanner (TLS) to characterize forest structure through the above-mentioned indices. The relationship between all of them was studied to assess the extent to which they relate and their capability to properly describe forest stands. A strong correlation was visible between LAI and the canopy density index (r = 0.87 to 0.91 depending on the extraction methods) despite the underevaluated values of the first. Even though more precise LAI estimates were expected from using co-registered multiple scans, the LAI variability proved to be low and correlations with the remaining indices weakened when compared to a single scan approach. An exception was canopy cover, a structural index that disregards the three-dimensionality of the canopy, with which the LAI obtained from multiple scans maintained a strong correlation. This suggests that multiple scanning leads to an unweighted oversampling of the scene, overshadowing its advantages in removing tree occlusions. Weak correlations were visible between classic forest structural indices (basal area density index, espacement index, denseness index) and the rest of the descriptors. Despite this exception, most of the forest indices showed average to strong correlations in-between each other. Therefore, we conclude that a better description of forest stands structure may be achieved through unsegmented single scan point cloud processing in both 3D and 2D space, optical data from the incorporated digital camera being a plus, but not an essential requirement.


Assuntos
Monitoramento Ambiental/métodos , Florestas , Lasers , Ecossistema , Luz , Tecnologia de Sensoriamento Remoto , Árvores
9.
Transplant Proc ; 51(5): 1549-1554, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31155190

RESUMO

OBJECTIVE: This study aimed to evaluate the effect of hepatic preconditioning with laser light in the presence of methylene blue (MB) in the liver ischemia-reperfusion injury process. METHOD: Forty male Wistar rats were divided into 8 experimental groups (n = 5). Saline (.5 mL) or MB (15 mg/kg) was injected intravenously (inferior vena cava). After 2 minutes, 660 nm laser light was applied at a dose of 112.5 DE. Fifteen minutes after the application of saline or MB, 1 hour partial ischemia followed by 15 minutes of reperfusion was applied when the rats were sacrificed. The mitochondrial function parameters (O2 consumption rates in states 3 and 4 and the respiratory control ratio), osmotic swelling, and determination of malondialdehyde were evaluated. Hepatic function was studied using the serum determination of the alanine aminotransferase and aspartate aminotransferase enzymes. RESULTS AND CONCLUSIONS: MB therapy alone showed the capacity of preserving the rate of oxygen consumption in the mitochondrial respiratory state of the group submitted to ischemia compared to the sham group. However, when combined with low-intensity laser therapy, it failed to replicate the relevant protective effects in relation to oxidative phosphorylation or the mitochondrial membrane ischemia/reperfusion injury. Whether or not MB was combined with laser treatment, it was shown to be efficient in reducing oxidative stress. In relation to alanine aminotransferase enzymes, whether or not laser treatment was combined with MB had a protective effect on the hepatic lesion, whereas in relation to aspartate aminotransferase enzymes only laser treatment was able to provide this protection.


Assuntos
Inibidores Enzimáticos/farmacologia , Lasers , Fígado/efeitos dos fármacos , Fígado/efeitos da radiação , Azul de Metileno/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Animais , Masculino , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/efeitos da radiação , Consumo de Oxigênio/efeitos dos fármacos , Consumo de Oxigênio/efeitos da radiação , Ratos , Ratos Wistar
10.
Food Chem ; 295: 327-333, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174765

RESUMO

Dual-pulse laser-induced breakdown spectroscopy (DPLIBS) and chemometric methods were used to predict chromium content in rice leaves, along with the purpose for increasing the detection sensitivity and accuracy. The influence of important parameters in DPLIBS were investigated and optimized. Then, partial least square (PLS) was used to establish chromium content prediction models, and the value of regression coefficient based on PLS was applied to determine feature variables. In addition, multivariate and univariate analysis were used to verify the modeling performance of selected feature variables. The results indicated that support vector machine model based on feature variables achieved the best performance, with correlation coefficient of 0.9946, root mean square error of 4.85 mg/kg and residual predictive deviation of 9.70 in prediction set. The proposed method provides a high-accuracy and fast approach for chromium content prediction in rice leaves, which could potentially be used for toxic and nutrient elements detection in food.


Assuntos
Cromo/análise , Oryza/química , Folhas de Planta/química , Análise Espectral/métodos , Poluentes Ambientais/análise , Lasers , Análise dos Mínimos Quadrados , Luz , Sensibilidade e Especificidade , Análise Espectral/estatística & dados numéricos , Máquina de Vetores de Suporte
11.
J Photochem Photobiol B ; 197: 111528, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31226526

RESUMO

Root conditioners are used to promote root surface biomodification to increase the success rate of root coverage. Citric acid and tetracycline are commonly used. There is recent indication for using antimicrobial photodynamic therapy (aPDT) with this purpose. The aim of this study is to evaluate the effects of citric acid/tetracycline gel and aPDT in root coverage of gingival recessions using subepithelial connective tissue graft. This parallel, double-blinded clinical trial enrolled 17 patients (60 recession defects; 20/group). Experimental groups were: Control group (SRP) - scaling and root planing only; Citric acid/tetracycline gel (CAT) group - SRP plus citric acid/tetracycline gel; aPDT - SRP, toluidine blue O (100 µl/ml) and red laser. At baseline and after 3, 6 and 12 months, the clinical parameters were evaluated: recession depth (RD), percentage of root coverage (%RC), keratinized tissue width (KTW), soft tissue thickness (STT), probing depth (PD), clinical attachment level (CAL), dentin hypersensitivity (HYPER) and esthetic perception by patient (EST). CAT group presented reduction in PD, CAL, RD, increase in KTW and STT, higher %RC (81.6%) and better esthetic in relation to SRP group (p < .05). aPDT treatment promoted CAL reduction, gain of KTW and STT and higher %RC (82.1%) in relation to SRP (57.7%) (p < .05). There was a reduction in dentin sensitivity in all groups. Complete root coverage was higher for CAT group (65%) and aPDT group (70%) in relation to SRP (30%) (p < .05). Root conditioning, with citric acid/tetracycline gel and aPDT, promotes better long-term clinical outcomes and root coverage after SCTG procedures.


Assuntos
Ácido Cítrico/química , Gengiva/transplante , Retração Gengival/terapia , Lasers , Tetraciclina/química , Adulto , Anti-Infecciosos/química , Anti-Infecciosos/uso terapêutico , Sensibilidade da Dentina/patologia , Método Duplo-Cego , Feminino , Géis/química , Gengiva/patologia , Retração Gengival/patologia , Regeneração Tecidual Guiada Periodontal , Humanos , Masculino , Pessoa de Meia-Idade , Fotoquimioterapia , Aplainamento Radicular , Tetraciclina/uso terapêutico , Raiz Dentária/cirurgia , Resultado do Tratamento , Adulto Jovem
12.
Graefes Arch Clin Exp Ophthalmol ; 257(8): 1709-1717, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31222405

RESUMO

BACKGROUND: Pigment epithelium-derived factor (PEDF)-derived 34-mer peptide (PEDF34, Asp44-Asn77) has anti-angiogenic activity but has limitations in clinical application because of an inverted bell-shaped dose-effect relationship and a short half-life. In this study, we attempted to mitigate these problems by mixing PEDF34 with type I collagen. METHODS: The anti-angiogenic activity of the PEDF34/atelocollagen mixture was evaluated by HUVEC tube formation assay and in a laser-induced choroidal neovascular (CNV) mouse model. PEDF34 and/or collagen were administrated using intravitreal injections or eye drops. CNV lesion size was quantified using FITC-dextran-perfused retinal whole mounts. Western blot analysis and inhibitor assays were used to define the action mechanisms of PEDF34 and the mixture. RESULTS: Collagen broadened the effective dose range of PEDF34 in the tube formation assay by > 250 times (from 0.2 to 50 nM). In the CNV model, five intravitreal injections of PEDF34 were required for therapeutic effect, whereas the mixture had a significant therapeutic effect following a single injection. Eye drops of the mixture showed significantly stronger CNV-suppressive effects than drops of PEDF34 alone. The anti-angiogenic activity of PEDF34 might be mediated by inhibition of ERK and JNK activation by VEGF, and collagen potentiated these effects. CONCLUSIONS: Collagen can serve as a carrier and reservoir of PEDF34. PEDF peptide/collagen mixture is easy to prepare than conventional methods for maintaining the therapeutic effect of PEDF peptide.


Assuntos
Corioide/patologia , Neovascularização de Coroide/tratamento farmacológico , Colágeno Tipo I/administração & dosagem , Proteínas do Olho/administração & dosagem , Fatores de Crescimento Neural/administração & dosagem , Serpinas/administração & dosagem , Animais , Células Cultivadas , Neovascularização de Coroide/metabolismo , Neovascularização de Coroide/patologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Feminino , Humanos , Lasers/efeitos adversos , Camundongos , Camundongos Endogâmicos C57BL , Soluções Oftálmicas , Inibidores de Proteases/administração & dosagem , Retina/patologia
13.
Nat Commun ; 10(1): 2589, 2019 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-31197138

RESUMO

X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.


Assuntos
Elétrons , Lasers , Microtúbulos/ultraestrutura , Imagem Molecular/métodos , Tubulina (Proteína)/ultraestrutura , Algoritmos , Cristalografia por Raios X/instrumentação , Cristalografia por Raios X/métodos , Processamento de Imagem Assistida por Computador , Imagem Molecular/instrumentação , Espalhamento de Radiação , Síncrotrons , Raios X
14.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 35(5): 385-392, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31223106

RESUMO

Objective To investigate the effect of low-level laser on the polarization and secretory phenotype of primary cultured M1 bone marrow-derived macrophages (BMDMs) in neuronal axons of dorsal root ganglion (DRG). Methods BMDMs were isolated and cultured, and lipopolysaccharide (LPS) combined with IFN-γ were used to induce M1 phenotype polarization of BMDMs, and then F4/80 and CD16/32 expression was detected by flow cytometry. The mature M1 type BMDMs were randomly divided into low-level laser group and control group. The laser exposure group was subjected to the laser treatments of 0.4J, 4J and 10J, and no laser was used in the control group. After 24 hours of laser exposure, the mRNA level of inducible nitric oxide synthase (iNOS) of M1 type BMDMs was detected by reverse transcription PCR, and the protein level of iNOS was detected by Western blot analysis. The levels of tumor necrosis factor alpha (TNF-α), interleukin-1ß (IL-1ß), brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in the supernatant of cultured cells were tested by ELISA. DRG neurons were cultured with the supernatant fluid of M1 type BMDMs, and immunofluorescence cytochemistry was employed to detect neuronal nuclei (NeuN) and ß-tubulin III expression of DRG neurons for determining the influence on the growth of DRG neuronal axons. Results Compared with the control group, the mRNA level of iNOS in M1 type BMDMs dramatically increased after 24 hours of low-level laser exposure. Among the 3 groups with different energy levels, the decrease of iNOS mRNA level was the most obvious in the group with 4J laser exposure. The protein levels of iNOS in the groups with 0.4J- and 4J- laser exposure were reduced more significantly than that in the control group, and the down-regulation was more prominent in the group with 4J laser exposure than that with 0.4J laser exposure. In addition, the secretion of TNF-α from M1 type BMDMs was reduced more significantly in the groups of 4J- and 10J- laser exposure than that in the control group. With regard to IL-1ß, its secretion was inhibited in all the laser exposure groups compared with the control group, and the suppression was more prominent in the groups of 0.4J- and 4J-laser exposure than that in the group of 10J-laser exposure. Furthermore, 4J-laser exposure significantly potentiated the secretion of BDNF and NGF in M1 type BMDMs compared with the control group. Moreover, co-culture with the supernatants from 4J- and 10J-laser exposure groups could significantly promote the growth of axons of DRG neurons. Conclusion Low-level laser exposure can inhibit the polarization of M1 type BMDM and the secretion of pro-inflammatory factor including TNF-α and IL-1ß. Besides, low-level laser exposure could contribute to the secretion of neurotrophic factors including BDNF and NGF, and promote the growth of DRG axon, and this effect is dose-dependent.


Assuntos
Axônios/fisiologia , Polaridade Celular , Gânglios Espinais/crescimento & desenvolvimento , Lasers , Macrófagos/citologia , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Células Cultivadas , Humanos , Interleucina-1beta/metabolismo , Lipopolissacarídeos , Macrófagos/efeitos da radiação , Fator de Crescimento Neural/metabolismo , Fator de Necrose Tumoral alfa/metabolismo
15.
Forensic Sci Int ; 301: 149-159, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31153992

RESUMO

This case report summarises the investigation of a death scene in the trunk of a car. Air sampling, laser-induced breakdown spectroscopy, and gas chromatography/mass spectrometry on samples of carpet and tyre well scrapings from the vehicle's trunk were utilised to confirm the presence of a human decompositional event even though no human remains were discovered in the vehicle. Air sampling has been used in numerous industries for many decades, but only recently has been applied to forensic investigations although it has been at the centre of controversy over the use of this technique in such cases. This report also describes the value of such investigative tools and points to the discovery of evidence, which, without the use of these techniques, would not have been identified.


Assuntos
Poluentes Atmosféricos/análise , Odorantes , Mudanças Depois da Morte , Automóveis , Dissulfeto de Carbono/análise , Tetracloreto de Carbono/análise , Clorofórmio/análise , Dissulfetos/análise , Elementos , Ácidos Graxos Voláteis/análise , Feminino , Pisos e Cobertura de Pisos , Ciências Forenses/métodos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Lasers , Análise Espectral , Sulfetos/análise
16.
Talanta ; 202: 317-322, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31171188

RESUMO

T4 polynucleotide kinase (T4 PNK) may catalyze the phosphorylation of 5'-hydroxyl termini in nucleic acids, which play a crucial role in DNA recombination, replication and damage repair. Here, a microchip electrophoresis laser induced fluorescence (MCE-LIF) method based on biochemical reaction was developed for the detection of T4 PNK activity and inhibitors. In this method, the single strand DNA (ssDNA) was hybridized with the 5-carboxyfluorescein (FAM) labeled single strand DNA (ssDNA-FAM) to form FAM labeled double-stranded DNA (dsDNA-FAM). In the presence of T4 PNK and adenosine triphosphate (ATP), T4 PNK catalyzes the transfer of γ-phosphate residues from ATP to the 5-hydroxyl terminal of dsDNA-FAM. The phosphorylated dsDNA-FAM can be gradually hydrolyzed by λexo to produce a FAM labeled single nucleotide fragment. Then the FAM labeled single nucleotide fragment and the unhydrolyzed dsDNA-FAM were separated by MCE, and two electrophoresis peaks appeared in the electrophoretogram. The detection of T4 PNK activity and inhibitors was realized by measuring the peak height of the FAM labeled single nucleotide fragment in electrophoretogram. This assay is very sensitive with a limit of detection of 0.002 U/mL, and it can be further used to screen the T4 PNK inhibitors.


Assuntos
Eletroforese em Microchip , Fluorescência , Lasers , Polinucleotídeo 5'-Hidroxiquinase/antagonistas & inibidores , Polinucleotídeo 5'-Hidroxiquinase/análise , Inibidores de Proteínas Quinases/farmacologia , Técnicas Biossensoriais , Hibridização de Ácido Nucleico , Polinucleotídeo 5'-Hidroxiquinase/metabolismo , Inibidores de Proteínas Quinases/química , Espectrometria de Fluorescência
17.
Journal of Oral Investigations ; 8(1): 86-99, jan.-jun. 2019.
Artigo em Português | BBO - Odontologia | ID: biblio-994736

RESUMO

A terapia endodôntica busca prevenir e quando necessário eliminar infecções endodônticas. A persistência de microrganismos ao preparo químico mecânico ou a medicação intracanal é considerada a principal causa dos insucessos do tratamento endodôntico. Sendo nestes casos o Enterococcus faecalis o microrganismo mais comumente encontrado. A terapia fotodinâmica (PDT) desponta desta maneira como uma promissora terapia coadjuvante ao tratamento endodôntico convencional para eliminar estes microrganismos persistentes. Esta terapia consiste na associação de uma fonte de luz específica a um fotossensibilizador na presença de oxigênio, produzindo assim espécies citotóxicas aos microrganismos. Assim, o presente artigo tem como objetivo realizar uma revisão de literatura sobre o uso da terapia fotodinâmica na endodontia, a qual demonstra-se efetiva como auxiliar durante o preparo do canal radicular(AU)


Endodontic therapy seeks to prevent and when necessary to eliminate endodontic infections. The persistence of microorganisms to the mechanical chemical preparation or the intracanal medication is considered the main cause of the failures of the endodontic treatment. In these cases Enterococcus faecalis is the most commonly found microorganism. Photodynamic therapy (PDT) emerges as a promising adjuvant therapy to conventional endodontic treatment to eliminate these persistent microorganisms. This therapy consists in the association of a specific light source with a photosensitizer in the presence of oxygen, producing cytotoxic species to the microorganisms. Thus, the present article aims at a review of the literature on the use of photodynamic therapy in endodontics, a test that is shown to assist in the preparation of the root canal(AU)


Assuntos
Fotoquimioterapia , Preparo de Canal Radicular , Endodontia , Lasers
18.
Food Chem ; 297: 124960, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31253301

RESUMO

Rice is the most consumed food worldwide, therefore its designation of origin (PDO) is very useful. Laser-induced breakdown spectroscopy (LIBS) is an interesting analytical technique for PDO certification, since it provides fast multielemental analysis requiring minimal sample treatment. In this work LIBS spectral data from rice analysis were evaluated for PDO certification of Argentine brown rice. Samples from two PDOs were analyzed by LIBS coupled to spark discharge. The selection of spectral data was accomplished by extreme gradient boosting (XGBoost), an algorithm currently used in machine learning, but rarely applied in chemical issues. Emission lines of C, Ca, Fe, Mg and Na were selected, and the best performance of classification were obtained using k-nearest neighbor (k-NN) algorithm. The developed method provided 84% of accuracy, 100% of sensitivity and 78% of specificity in classification of test samples. Furthermore, it is simple, clean and can be easily applied for rice certification.


Assuntos
Análise de Alimentos/métodos , Oryza/química , Análise Espectral/métodos , Algoritmos , Argentina , Análise de Alimentos/estatística & dados numéricos , Lasers , Metais/análise , Metais/química , Análise Espectral/estatística & dados numéricos
19.
Anal Chim Acta ; 1067: 48-55, 2019 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-31047148

RESUMO

Immunoassay is a powerful technique to identify and quantify biological molecules, which base on the specificity and selectivity of antigen-antibody interaction. Impedance-based immunosensor has recently shown a great potential to provide rapid and label-free detections. However, the conventional impedance-based immunosensors rely on dedicated electrochemical measurement interface which involves expensive fabrication procedures such as gold deposition and photolithography. In this work, we propose an ultra-low-cost and high processing efficiency platform for impedance-based immunosensing. With effortless operations of direct-laser-writing, an impedance-based immunoassay can be fabricated within 5 min in standard laboratories. The as-fabricated devices have shown great stability and a high device-to-device uniformity. In order to further validate impedance sensing system's performance, finite element analysis and impedance equivalent model analysis were performed. The measured data was consistent with the simulation results. With the standard gold electrodes surface bio-functionalization procedures, the disposable immunoassay can detect anti-IgG down to 10 ng/ml.


Assuntos
Anticorpos/análise , Impedância Elétrica , Imunoensaio , Lasers , Impressão , Anticorpos/imunologia , Eletrodos , Ouro/química , Imunoensaio/economia , Imunoglobulina G/imunologia , Propriedades de Superfície
20.
J Photochem Photobiol B ; 196: 111512, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31129505

RESUMO

Cancer is a leading cause of death worldwide, and doxorubicin (DOX) has become one of the most commonly prescribed drugs. Stem cell (SC) therapy is proving to be a promising strategy to alleviate DOX adverse effects on non-cancerous cells. However, the drug also has a toxic action on SCs, reducing the efficiency of cell therapy from a preventive view. The present study shows that the DOX toxicity in mesenchymal SCs (MSCs) can be partially overcome by low-level laser irradiation (LLLI). To achieve this, we applied the low-level red laser (wavelength: 660 nm; output power: 30 mW; laser beam: 0.028 cm2; irradiation: 1.07 mW/cm2; Ga-Al-As Photon Laser III, DMC, São Paulo, Brazil) in rat adipose tissue-derived MSCs before their exposure to different DOX concentrations. Results revealed that the DOX reduced the viability and adenosine triphosphate level of MSCs. These findings were followed by significantly increased apoptosis as well as oxidative stress in the MSCs. Interestingly, LLLI at the dose of 0.2 J alleviated the effects of DOX on cell viability and apoptosis, and inhibited oxidative stress in the MSCs. In summary, this study provides a crucial step toward the future application of LLLI as a protective approach against DOX-induced toxicity in MSCs, particularly cell death. This study also lays the groundwork for further investigation into the role of oxidative stress and inflammation as an instructive milieu for cell protection.


Assuntos
Apoptose/efeitos da radiação , Doxorrubicina/farmacologia , Lasers , Trifosfato de Adenosina/metabolismo , Tecido Adiposo/citologia , Animais , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Células Cultivadas , Citocinas/metabolismo , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/efeitos da radiação , Ratos
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