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1.
Planta ; 254(3): 54, 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34410495

RESUMO

MAIN CONCLUSION: The expression of full-length cDNAs encoding lavender AGAMOUS-like (LaAG-like) and SEPALLATA3-like (LaSEP3-like) transcription factors induces early flowering and impacts the leaf morphology at a strong expression level in Arabidopsis. Lavandula angustifolia is widely cultivated as an ornamental plant due to its attractive flower structure, and as a source of valuable essential oils for use in cosmetics, alternative medicines, and culinary products. We recently employed RNA-Seq and transcript profiling to describe a number of transcription factors (TFs) that potentially control flower development in this plant. In this study, we investigated the roles of two TFs, LaAGAMOUS-like (LaAG-like) and LaSEPALLATA3-like (LaSEP3-like), that exhibited substantial homology to Arabidopsis thaliana floral development genes, AGAMOUS and SEPALLATA3, respectively, in flowering initiation in Arabidopsis. We stably and constitutively expressed LaAG-like and LaSEP3-like cDNAs in separate Arabidopsis plants. All transgenic plants flowered earlier than the wild-type controls. However, plants that modestly overexpressed the gene were phenotypically normal, while those that strongly expressed the transgene developed curly leaves. We also assessed the expression of five endogenous flowering time regulating genes, from which high expression of Flowering Locus T (AtFT) mRNA in both LaAG-like (type-I and -II) and LaSEP3-like (type-I), and Leafy (AtLFY) mRNAs in LaSEP3-like (type-I) transgenic plants were detected, compared to wild-type controls. Our results suggest that with controlled expression, lavender AG-like and SEP3-like genes are potentially useful for the regulation of flowering time in commercial lavender species, and could be used for plant improvement studies through molecular genetics and targeted breeding programs.


Assuntos
Proteínas de Arabidopsis , Arabidopsis , Lavandula , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Flores/genética , Flores/metabolismo , Regulação da Expressão Gênica de Plantas , Lavandula/metabolismo , Proteínas de Domínio MADS/genética , Melhoramento Vegetal , Folhas de Planta/genética , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Proteínas Repressoras
2.
Molecules ; 26(16)2021 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-34443525

RESUMO

The epidemiology of yeast infections and resistance to available antifungal drugs are rapidly increasing, and non-albicans Candida species and rare yeast species are increasingly emerging as major opportunistic pathogens. In order to identify new strategies to counter the threat of antimicrobial resistant microorganisms, essential oils (EOs) have become an important potential in the treatment of fungal infections. EOs and their bioactive pure compounds have been found to exhibit a wide range of remarkable biological activities. We investigated the in vitro antifungal activity of nine commercial EOs such as Thymus vulgaris (thyme red), Origanum vulgare (oregano), Lavandula vera (lavender), Pinus sylvestris (pine), Foeniculum vulgare (fennel), Melissa officinalis (lemon balm), Salvia officinalis (sage), Eugenia caryophyllata (clove) and Pelargonium asperum (geranium), and some of their main components (α-pinene, carvacrol, citronellal, eugenol, γ-terpinene, linalool, linalylacetate, terpinen-4-ol, thymol) against non-albicans Candida strains and uncommon yeasts. The EOs were analyzed by GC-MS, and their antifungal properties were evaluated by minimum inhibitory concentration and minimum fungicidal concentration parameters, in accordance with CLSI guidelines, with some modifications for EOs. Pine exhibited strong antifungal activity against the selected non-albicans Candida isolates and uncommon yeasts. In addition, lemon balm EOs and α-pinene exhibited strong antifungal activity against the selected non-albicans Candida yeasts. Thymol inhibited the growth of all uncommon yeasts. These data showed a promising potential application of EOs as natural adjuvant for management of infections by emerging non-albicans Candida species and uncommon pathogenic yeasts.


Assuntos
Antifúngicos/química , Candida/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Antifúngicos/farmacologia , Candida/patogenicidade , Candida albicans/efeitos dos fármacos , Candida albicans/patogenicidade , Candidíase/tratamento farmacológico , Candidíase/microbiologia , Farmacorresistência Fúngica/efeitos dos fármacos , Foeniculum/química , Humanos , Lavandula/química , Melissa/química , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Origanum/química , Pinus sylvestris/química , Óleos Vegetais/química , Salvia officinalis/química , Syzygium/química , Thymus (Planta)/química
3.
Poult Sci ; 100(9): 101344, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34358952

RESUMO

The unprecedented rate of global warming requires more immaculate strategies to fight the heat stress and its detrimental effects on poultry sector. Nutritional strategies, particularly herbal extracts, play a crucial importance in this scenario. Therefore, this study was conducted to evaluate the effects of a novel selected mixture (hereafter refers to as Stress-Bio-Max, SBM) comprising St John's wort, lavender, Melissa officinalis extracts, betaine, and Vit C on heat-stressed broilers. A total of 300 male broiler chicks were allocated to 4 treatments: T1, Control (no SBM in drinking water); T2, 0.25 mL/L of SBM in drinking water; T3, 0.5 and T4, 1 mL/L of SBM, which applied since d 32. The birds underwent heat stress (40 ± 1) daily from d 35 to 42 of the experiment (2 pm-8 pm). The results show the superior weight gain of T2 and T3 from d 7 to 42 compared to other groups, 72.5 and 72.7 g/d, respectively (P < 0.05). Corticosterone level was higher on d 35 in T1 (4.674 ng/mL, P < 0.05) and lower in T2 and T3 (2.64 and 2.952 ng/mL, respectively, P < 0.05); T2 and T3 also caused the lowest concentrations of corticosterone on d 40 (5.198 and 6.458 ng/mL, respectively, P < 0.05). The superior triiodothyronine levels belonged to T2 (0.935 ng/mL, P < 0.05) on d 35 and T2 and T3 on d 40 (0.699 and 0.582 ng/mL, respectively, P < 0.05). T2, T3, and T4 caused a rise in glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities (P < 0.05). A significant higher amount of total antioxidant capacity (TAC) belonged to the groups treating with SBM (P < 0.05). The SBM in T2 and T3 resulted in the lowest levels of malondialdehyde (MDA), aspartate transaminase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Results from this study indicate that SBM may alleviate the negative impacts associated with heat stress in broiler chickens.


Assuntos
Hypericum , Lavandula , Melissa , Animais , Ácido Ascórbico , Betaína , Galinhas , Resposta ao Choque Térmico
4.
Appl Environ Microbiol ; 87(19): e0109921, 2021 09 10.
Artigo em Inglês | MEDLINE | ID: mdl-34319799

RESUMO

New approaches for the control of Campylobacter jejuni biofilms in the food industry are being studied intensively. Natural products are promising alternative antimicrobial substances to control biofilm production, with particular emphasis on plant extracts. Dried flowers of Lavandula angustifolia were used to produce essential oil (LEO), an ethanol extract (LEF), and an ethanol extract of Lavandula postdistillation waste material (LEW). The chemical compositions determined for these Lavandula preparations included seven major compounds that were selected for further testing. These were tested against C. jejuni for biofilm degradation and removal. Next-generation sequencing was used to study the molecular mechanisms underlying LEO actions against C. jejuni adhesion and motility. Analysis of LEO revealed 1,8-cineol, linalool, and linalyl acetate as the main components. For LEF and LEW, the main components were phenolic acid glycosides, with flavonoids rarely present. The MICs of the Lavandula preparations and pure compounds against C. jejuni ranged from 0.2 mg/ml to 1 mg/ml. LEO showed the strongest biofilm degradation. The reduction of C. jejuni adhesion was ≥1 log10 CFU/ml, which satisfies European Food Safety Authority recommendations. Lavandula preparations reduced C. jejuni motility by almost 50%, which consequently can impact biofilm formation. These data are in line with the transcriptome analysis of C. jejuni, which indicated that LEO downregulated genes important for biofilm formation. LEW also showed good antibacterial and antibiofilm effects, particularly against adhesion and motility mechanisms. This defines an innovative approach using alternative strategies and novel targets to combat bacterial biofilm formation and, hence, the potential to develop new effective agents with biofilm-degrading activities. IMPORTANCE The Lavandula preparations used in this study are found to be effective against C. jejuni, a common foodborne pathogen. They show antibiofilm properties at subinhibitory concentrations in terms of promoting biofilm degradation and inhibiting cell adhesion and motility, which are involved in the initial steps of biofilm formation. These results are confirmed by transcriptome analysis, which highlights the effect of Lavandula essential oil on C. jejuni biofilm properties. We show that the waste material from the hydrodistillation of Lavandula has particular antibiofilm effects, suggesting that it has potential for reuse for industrial purposes. This study highlights the need for efforts directed toward such innovative approaches and alternative strategies against biofilm formation and maintenance by developing new naturally derived agents with antibiofilm activities.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Campylobacter jejuni/efeitos dos fármacos , Lavandula , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Óleos Vegetais/farmacologia , Antibacterianos/química , Aderência Bacteriana/efeitos dos fármacos , Campylobacter jejuni/genética , Campylobacter jejuni/crescimento & desenvolvimento , Campylobacter jejuni/fisiologia , Flavonoides/análise , Flavonoides/farmacologia , Flores , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Óleos Voláteis/química , Compostos Fitoquímicos/análise , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/química , Óleos Vegetais/química , Resíduos
5.
Complement Ther Clin Pract ; 44: 101430, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34217127

RESUMO

OBJECTIVE: to determine massage lavender essential oil on neuropathic pain and quality of life in diabetic patients. METHODS: A randomized three-group control trial with the pre-post design was performed from 2019 to 2020 on 75 diabetic neuropathic patients. The patients were randomly allocated into aromatherapy (n = 26), placebo (n = 26), and control (n = 26) groups. Patients in the intervention group used 2.5 cc of 3% lavender oil on their feet as a gentle massage for 10 min every night before bedtime for a month. Data collected using the Visual analog scale (VAS), Douleur Neuropathic 4 (DN4) and Quality of Life Questionnaire (SF36). RESULTS: The mean difference of pain scores in short-term and long-term in the aromatherapy group was significantly reduced compared to the placebo and control groups (P < 0.001). In addition, after four weeks, a significant increase was found in the QoL domains in the aromatherapy group (P < 0.001). CONCLUSION: Aromatherapy massage with lavender oil helped reduce neuropathic pain two to four weeks after the intervention and improved the patients' QoL without causing any side effects. Thus, nurses are recommended to use it as a complementary method to reduce neuropathic pain and improve patients' QoL.


Assuntos
Aromaterapia , Diabetes Mellitus , Neuropatias Diabéticas , Lavandula , Neuralgia , Óleos Voláteis , Humanos , Massagem , Neuralgia/tratamento farmacológico , Óleos Voláteis/uso terapêutico , Óleos Vegetais/uso terapêutico , Qualidade de Vida
6.
Molecules ; 26(13)2021 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-34202776

RESUMO

The chemical composition, antioxidant activity, and antimicrobial properties of three commercially available essential oils: rosemary (REO), lavender (LEO), and mint (MEO), were determined in the current study. Our data revealed that the major components of REO, MEO, and LEO were 1,8-cineole (40.4%), menthol (40.1%), and linalool acetate (35.0%), respectively. The highest DPPH radical-scavenging activity was identified in MEO (36.85 ± 0.49%) among the investigated EOs. Regarding antimicrobial activities, we found that LEO had the strongest inhibitory efficiencies against the growth of Pseudomonas aeruginosa and Candida (C.) tropicalis, MEO against Salmonella (S.) enterica, and REO against Staphylococcus (S.) aureus. The strongest antifungal activity was displayed by mint EO, which totally inhibited the growth of Penicillium (P.) expansum and P. crustosum in all concentrations; the growth of P. citrinum was completely suppressed only by the lowest MEO concentration. The lowest minimal inhibitory concentrations (MICs) against S. enterica, S. aureus, and C. krusei were assessed for MEO. In situ analysis on the bread model showed that 125 µL/L of REO exhibited the lowest mycelial growth inhibition (MGI) of P. citrinum, and 500 µL/L of MEO caused the highest MGI of P. crustosum. Our results allow us to make conclusion that the analysed EOs have promising potential for use as innovative agents in the storage of bakery products in order to extend their shelf-life.


Assuntos
Anti-Infecciosos/farmacologia , Pão/microbiologia , Lavandula/química , Mentha/química , Óleos Voláteis/farmacologia , Penicillium/crescimento & desenvolvimento , Rosmarinus/química , Anti-Infecciosos/química , Bactérias/crescimento & desenvolvimento , Candida tropicalis/crescimento & desenvolvimento , Óleos Voláteis/química
7.
Molecules ; 26(14)2021 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-34299656

RESUMO

Chemical composition, antioxidant capacity, and antimicrobial activity of lavender essential oils (LEOs) extracted from three different varieties of Lavandula angustifolia Mill. (1-Moldoveanca 4, 2-Vis magic 10, and 3-Alba 7) have been determined. These plants previously patented in the Republic of Moldova were cultivated in an organic agriculture system in the northeastern part of Romania and then harvested in 3 consecutive years (2017-2019) to obtain the essential oils. From the inflorescences in the complete flowering stage, the LEOs were extracted by hydrodistillation. Then, their composition was analyzed by gas chromatography coupled with mass spectrometry (GC-MS) and by Fourier Transformed Infrared spectroscopy (FT-IR). The major identified constituents are as follows: linalool (1: 32.19-46.83%; 2: 29.93-30.97%; 3: 31.97-33.77%), linalyl acetate (1: 17.70-35.18%; 2: 27.55-37.13%; 3: 28.03-35.32%), and terpinen-4-ol (1: 3.63-7.70%; 2: 3.06-7.16%; 3: 3.10-6.53%). The antioxidant capacity as determined by ABTS and DPPH assays indicates inhibition, with the highest activity obtained for LEO var. Alba 7 from 2019. The in vitro antimicrobial activities of the LEOs and combinations were investigated as well, by using the disk diffusion method and minimum inhibitory concentration (MIC) against the Gram-positive bacterial strain Staphylococcus aureus (ATCC 6538), Gram-negative Pseudomonas aeruginosa (ATCC 27858), Escherichia coli (ATCC 25922), the yeast Candida albicans (ATCC 10231), and clinical isolates. Our results have shown that LEOs obtained from the three studied varieties of L. angustifolia manifest significant bactericidal effects against tested microorganisms (Staphylococcus aureus and Escherichia coli), and antifungal effects against Candida albicans. The mixture of LEOs (Var. Alba 7) and geranium, respectively, in tea tree EOs, in different ratios, showed a significant enhancement of the antibacterial effect against all the studied strains, except Pseudomonas aeruginosa.


Assuntos
Anti-Infecciosos , Antioxidantes , Bactérias/crescimento & desenvolvimento , Candida albicans/crescimento & desenvolvimento , Lavandula/química , Óleos Voláteis , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia
8.
Nurs Womens Health ; 25(4): 286-295, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34153228

RESUMO

OBJECTIVE: To explore potential clinical applications, based on evidence and a nurse-driven test of change, of using lavender aromatherapy for preoperative anxiety as an intervention complementary to standard preoperative care. DESIGN: A pre- versus postaromatherapy comparison using a visual analog scale (VAS). SETTING: The preoperative department at a level 2 trauma hospital with 544 beds. PARTICIPANTS: Forty-four surgical patients, including 29 female participants and 15 male participants. INTERVENTION/MEASUREMENTS: Participants reported their anxiety on a VAS before receiving a lavender aromatherapy inhaler. Anxiety scores were measured again after receiving the lavender aromatherapy and shortly before participants left the preoperative area for surgery. A pre-post comparison of the two VAS anxiety measurements before and after receiving the lavender aromatherapy was completed, analyzed, and is discussed. RESULTS: Mean anxiety scores were calculated for the pre- and postaromatherapy groups. Forty-eight percent of female participants (n = 29) reported a decrease in their anxiety after receiving preoperative lavender aromatherapy. Female participants reported higher preoperative anxiety scores and a larger decrease in their mean anxiety scores after receiving lavender aromatherapy when compared to male participants. CONCLUSION: The use of a nurse-driven complementary intervention in the preoperative area was associated with a decrease in mean anxiety scores among female patients about to undergo elective surgery. However, cause and effect cannot be determined because of a lack of a control group and randomization. Opportunities exist with support from seasoned staff for nurses to incorporate safe, evidence-based complementary interventions into the current standard of care for preoperative anxiety.


Assuntos
Ansiedade/prevenção & controle , Aromaterapia/métodos , Lavandula/química , Óleos Voláteis/uso terapêutico , Cuidados Pré-Operatórios/métodos , Ansiedade/psicologia , Transtornos de Ansiedade , Humanos , Óleos Vegetais , Resultado do Tratamento
9.
Can J Microbiol ; 67(10): 724-736, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34153193

RESUMO

Lavender essential oil (LEO), a natural antimicrobial agent, is generally recognized as safe and effective in the inhibition of phytopathogenic fungi. Direct contact and fumigation (in vivo and in vitro) were used to study the fungistatic effect of LEO on Monilinia fructicola. Additionally, the effect on the ultrastructure of cells and the degree of destruction of the cell membrane of M. fructicola were revealed. In addition, the effects of LEO on the expression levels of apoptosis-related genes in M. fructicola cells were detected, and GC-MS was used to analyze the main components of LEO. LEO had a good inhibitory efficacy against M. fructicola in flat peaches, with almost complete growth inhibition at 800 µL/L. These effects were associated with the leakage of cytoplasmic contents, hyphal distortion, and spore disruption. Moreover, the expression of apoptosis RTG1 and RLM1 genes increased with LEO treatment. These results demonstrate that LEO can inhibit M. fructicola by inducing cytoplasmic membrane damage and cell apoptosis in fungi, and that the major ingredients of LEO are monoterpenes and sesquiterpenes, which are presumed to contribute to the inhibitory effects.


Assuntos
Ascomicetos , Lavandula , Óleos Voláteis , Prunus persica , Antifúngicos/farmacologia , Ascomicetos/genética , Frutas , Óleos Voláteis/farmacologia
10.
Food Chem ; 359: 129963, 2021 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-33951609

RESUMO

This research studies the application of a specific nanoemulsion as anti-Escherichia coli agent. The specific mixture was generated by a simplex-centroid design. Physicochemical parameters such as droplet average diameter, pH, viscosity, density, turbidity, whitening index, refractive index, stability (thermal, physical, and osmotic stability), and antibacterial activity kinetic, have been assessed. The mixture nanoemulsions had droplet diameters significantly smaller than those of clove or cinnamon nanoemulsions. Individual and mixture essential oils nanoemulsion exhibited appropriate stability under pH, thermal, and ionic stress as well as after mid-term storage. Antibacterial activity kinetic revealed the fast and pronounced efficacy of mixture nanoemulsions on E. coli (reach 98% of growth inhibition), especially for the nanoemulsion composed of 50% essential oil in the dispersed phase upon 20 days of storage. All data considered, the actual work evidences the promising advantages of using specific nanoemulsions as delivery systems of antibacterial agents in the beverage and food industry.


Assuntos
Anti-Infecciosos/farmacologia , Cinnamomum zeylanicum/química , Emulsões/química , Escherichia coli/efeitos dos fármacos , Lavandula/química , Óleos Voláteis/química , Syzygium/química , Óleos Voláteis/farmacologia
11.
Complement Ther Clin Pract ; 43: 101391, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33865080

RESUMO

OBJECTIVES: This paper aims to review articles that have evaluated the role of essential oil therapy in patients with rheumatic diseases. METHODOLOGY: Systematic review. No study design or language limitation was applied. RESULTS: We have identified 13 articles, most of them were used in osteoarthritis (n = 4), rheumatoid arthritis (n = 3) and fibromyalgia (n = 3). Two studies included patients with RA and OA (n = 2). The number of people involved in the study varied from 9 to 162, where female sex was observed in 60-100%. The age of the patients ranged from 36 to 78.3 years old and disease duration 1-11.67 years. The lavender essential oil was the most used, and then Ginger oil, Rosemary oil, and Rosmarinus officinalis. Time of oil use varied from 2 to 12 weeks. Importantly, all studies but one (91.6%) have demonstrated the efficacy of aromatherapy. CONCLUSION: There are few reports on essential oils in rheumatic disease, mainly osteoarthritis, rheumatoid arthritis, and fibromyalgia. All but one study have showed the efficacy of this complementary therapy.


Assuntos
Aromaterapia , Lavandula , Óleos Voláteis , Doenças Reumáticas , Rosmarinus , Adulto , Idoso , Feminino , Humanos , Pessoa de Meia-Idade , Óleos Voláteis/uso terapêutico , Doenças Reumáticas/tratamento farmacológico
12.
Molecules ; 26(8)2021 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-33920647

RESUMO

Lavandula angustifolia Mill. (lavender) is an essential-oil-bearing plant in the Lamiaceae family. Volatile oil produced through the steam distillation of lavender was examined to establish the essential oil yield and aromatic profile from each portion of the plant-namely, the corolla, calyx, leaf, and whole flowering top. The resulting essential oils were analyzed by GC-FID and GC-MS. The different plant parts generally shared similar compounds but in varying relative percentages. Aromatic profiles of the whole flowering top and calyx were similar, with prominent compounds being linalool acetate (34.3%, 32.0%), linalool (26.5%, 32.9%), lavandulyl acetate (5.6%, 4.9%), terpinen-4-ol (5.3%, 7.0%), and (Z)-ß-ocimene (4.5%, 5.4%), respectively. Aromatic profiles for the corolla and leaf were unique. Prominent aromatic compounds of the corolla included linalool acetate (18.4%), linalool (10.8%), epi-α-cadinol (10.0%), borneol (7.3%), and lavandulyl acetate (6.3%). Prominent aromatic compounds of the leaf included epi-α-cadinol (19.8%), γ-cadinene (11.0%), borneol (6.0%), caryophyllene oxide (4.9%), and bornyl acetate (4.8%). Complete profiles and essential oil yields of corolla, calyx, leaf, and whole flowering top were established. This study establishes the influence the corolla, calyx, and leaf exert on the aromatic profile of the whole flowering top and provides insight into authentication of lavender essential oil.


Assuntos
Flores/química , Lavandula/química , Óleos Voláteis/química , Folhas de Planta/química , Acetatos/química , Destilação , Monoterpenos/química , Óleos Voláteis/classificação , Óleos Vegetais/química , Terpenos/química
13.
Molecules ; 26(6)2021 Mar 19.
Artigo em Inglês | MEDLINE | ID: mdl-33808553

RESUMO

Infections associated with the emergence of multidrug resistance and mosquito-borne diseases have resulted in serious crises associated with high mortality and left behind a huge socioeconomic burden. The chemical investigation of Lavandulacoronopifolia aerial parts extract using HPLC-MS/MS led to the tentative identification of 46 compounds belonging to phenolic acids, flavonoids and their glycosides, and biflavonoids. The extract displayed larvicidal activity against Culex pipiens larvae (LC50 = 29.08 µg/mL at 72 h). It significantly inhibited cytochrome P-450 monooxygenase (CYP450), acetylcholinesterase (AChE), and carboxylesterase (CarE) enzymes with the comparable pattern to the control group, which could explain the mode of larvae toxification. The extract also inhibited the biofilm formation of Pseudomonas aeruginosa by 17-38% at different Minimum Inhibitory Concentrations (MICs) (0.5-0.125 mg/mL) while the activity was doubled when combined with ciprofloxacin (ratio = 1:1 v:v). In conclusion, the wild plant, L.coronopifolia, can be considered a promising natural source against resistant bacteria and infectious carriers.


Assuntos
Antibacterianos , Biofilmes/efeitos dos fármacos , Culex/crescimento & desenvolvimento , Inseticidas , Lavandula/química , Extratos Vegetais , Folhas de Planta/química , Pseudomonas aeruginosa/fisiologia , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Biofilmes/crescimento & desenvolvimento , Inseticidas/química , Inseticidas/farmacologia , Extratos Vegetais/química , Extratos Vegetais/farmacologia
14.
Molecules ; 26(9)2021 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-33922508

RESUMO

Lavandula angustifolia, one of the most popular medicinal plants, is the source of a bioactive essential oil characterized by a wide spectrum of biological activity, e.g., antiseptic, analgesic, and anticancer effects. In dermatology, the oil helps to relieve skin inflammation and exhibit wound healing potential. However, the mechanism of action of the lavender oil depends on its composition, which in turn is dependent on the origin and growing conditions. Our study aimed to compare the composition and proregenerative properties of the commercially-available narrow-leaved lavender oil produced in Provence, France, with the oil obtained from the narrow-leaved lavender cultivated locally in Poland. GC/MS analysis showed that self-manufactured essential oil had lower linalool content than commercial oil (23.2 vs. 40.2%), comparable linalyl acetate content (40.6 vs. 44%), while the proportion of lavandulyl acetate was significantly higher (23.2 vs. 5.5%). To determine the influence of lavender oil on the production of proinflammatory cytokines and proregenerative growth factors, gene expression of the selected signaling molecules by HaCaT cells was investigated using real-time PCR. Results showed a concentration-dependent effect of lavender oils on the production of IL-6, IL-8, and VEGF by the keratinocyte cell line. Finally, the potential of the lavender oil to increase the production of VEGF, the most important angiogenic factor, with the in-house preparation performing significantly better in the in vitro cell models was identified.


Assuntos
Lavandula/química , Óleos Voláteis/química , Linhagem Celular Tumoral , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Óleos Voláteis/farmacologia , Cicatrização/efeitos dos fármacos
15.
16.
Planta Med ; 87(10-11): 754-763, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33882591

RESUMO

To fight the rising resistance of microorganisms to antibiotics, a strategy followed by several researchers is to focus on natural compounds, such as essential oils, as a source of potent antibacterial compounds. These last decades, hundreds of original papers have been written about microbiological assays that prove the antibacterial activity of essential oils and their use in the medical field. But can we really compare all the data available in the literature when the raw material, the microbiological assays, and/or the strains are different from one article to another? This review will point out the differences and the inadequate practices found in published articles that tested 2 lesser-studied essential oils-Spanish lavender and the ajowan-by the broth dilution method against Staphylococcus aureus, a human pathogenic bacterium. Many pitfalls were found in the literature, for example, a variable chemical composition rarely underlined by the authors, unidentified strains or clinical strains used without a related antibiogram, a lack of quality controls, and the assertion of questionable positive results. At last, some general guidelines that should be followed by every scientific researcher will be discussed.


Assuntos
Anti-Infecciosos , Carum , Lavandula , Óleos Voláteis , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Óleos Voláteis/farmacologia
17.
J Pharm Biomed Anal ; 199: 114050, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33831738

RESUMO

Currently, the quality of lavender (Lavandula angustifolia Mill.) essential oil (LEO) is defined and regulated based on standards and methods established by regulatory authorities. Unfortunately, these existing standards and methods are not sufficient for LEO quality evaluation due to the complexity of LEO and adulteration encouraged by a burgeoning market. This study provides an efficient and reliable method for LEO quality assessment and adulteration detection. After a comprehensive investigation, involving a large set of LEO samples (n = 72) analyzed by multiple techniques (GC/MS, GC/Q-ToF, NMR, and chemometric analysis), a new approach named Q-Index was proposed. Fourteen marker compounds, along with trans-furano-linalool oxide acetate (an indicator of synthetic compound adulteration in LEO), were identified. These marker compounds played significant roles in discriminating the adulterated samples from the authentic LEOs. Calculation of the Q-Index value using the identified marker compounds permitted the detection of fraudulent samples. As demonstrated, all the authentic LEOs exhibited high Q-Index values (>100), whereas the adulterated or poor-quality samples displayed low Q-Index values (<100). The NMR-based chemometric analysis, which served as an independent and complementary approach to the GC/MS and Q-Index methods, was applied in order to assess the validity of the Q-Index method. Overall, the results obtained from different methods were in good agreement. Moreover, compared to the NMR method, the Q-Index approach possessed greater sensitivity in detecting LEO adulteration associated with the addition of synthetic compounds. Results of this study demonstrated that the Q-Index method could be successfully applied for LEO quality assessment and adulteration detection. This approach may have a significant potential to improve quality control for the LEO industry.


Assuntos
Lavandula , Óleos Voláteis , Contaminação de Medicamentos , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Óleos Vegetais
18.
Curr Allergy Asthma Rep ; 21(4): 26, 2021 03 29.
Artigo em Inglês | MEDLINE | ID: mdl-33779825

RESUMO

PURPOSE OF REVIEW: This article aims to summarize some recent trends in occupational allergic contact dermatitis (ACD), including dermatitis related to pandemic-level personal protective equipment in healthcare workers, hazards patients may experience when working from home, and occupational perspectives on the recent American Contact Dermatitis Society (ACDS) allergens of the year and ACDS Core Allergen Series updates. RECENT FINDINGS: Recent ACDS Allergens of the Year may be particularly relevant to healthcare workers, including isobornyl acrylate, which is present in glucose sensors and propylene glycol present in hand cleansers and disinfectants. Lavender, limonene, and linalool, all of which are new additions to the ACDS Core Allergen Series, have been reported as causes for occupational ACD in massage therapists and aromatherapists. Isothiazolinone allergy continues to rise in both consumer and occupational settings. Finally, the COVID-19 pandemic has resulted in a wave of occupational ACD in healthcare workers to personal protective equipment, and revealed new potential allergens for individuals working from home. Occupational allergic contact dermatitis continues to exert a significant occupational disease burden. Remaining aware of the current trends in allergens may allow for earlier recognition, diagnosis, and treatment, subsequently helping our patients to work in healthier and safer environments.


Assuntos
Alérgenos/efeitos adversos , COVID-19/epidemiologia , Dermatite Alérgica de Contato/diagnóstico , Dermatite Ocupacional/diagnóstico , Acrilatos , Monoterpenos Acíclicos/efeitos adversos , Alergia e Imunologia/tendências , Canfanos , Dermatite Ocupacional/etiologia , Dermatologia/tendências , Pessoal de Saúde , Humanos , Lavandula/efeitos adversos , Limoneno/efeitos adversos , Pandemias , Testes do Emplastro/efeitos adversos , Propilenoglicol , Sociedades Médicas , Estados Unidos
19.
Molecules ; 26(4)2021 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-33670315

RESUMO

Arabian flora is a rich source of bioactive compounds. In this study, we investigated three aromatic plant species with the aim of finding valuable sources of antimicrobial agents against common pathogenic microorganisms. We focused especially on microorganisms, which cause outbreaks of infectious disease during mass gatherings and pilgrimages season in Saudi Arabia. The essential oils of three aromatic plant species were hydrodistilled from flowering aerial parts of Lavandula pubescens Decne. and Pulicaria incisa subsp. candolleana E.Gamal-Eldin, and from leaves, stems, ripe and unripe fruits of Juniperus procera Hochst. Ex Endl. They were subsequently analyzed by gas chromatography-mass spectrometry (GC-MS). The main constituents of L. pubescens were found to be carvacrol (55.7%), methyl carvacrol (13.4%), and ß-bisabolene (9.1%). P. incisa subsp. Candolleana essential oil was rich in linalool (33.0%), chrysanthenone (10.3%), eugenol (8.9%), and cis-chrysanthenol (8.0%); the major components of J. procera essential oil were α-pinene (31.3-62.5%) and δ-3-carene (7.3-30.3%). These essential oils were tested against thirteen American Type Culture Collection (ATCC) strains of Gram-positive and Gram-negative bacteria using the agar diffusion assay. The only effective essential oil was that of L. pubescens and the most sensitive strains were Acinetobacter baumannii, Salmonella typhimurium, Shigella sonnei, Enterococcus faecalis and Staphylococcus epidermidis. Carvacrol, the major constituent of L. pubescens, was tested on these strains and was compared with vancomycin, amikacin, and ciprofloxacin. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assays of L. pubescens essential oil and carvacrol revealed that Gram-negative strains were more susceptible than the Gram-positive ones.


Assuntos
Juniperus/química , Lavandula/química , Óleos Voláteis/química , Óleos Vegetais/química , Pulicaria/química , Antibacterianos/química , Antibacterianos/farmacologia , Cimenos/química , Cimenos/farmacologia , Enterococcus faecalis/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Óleos Voláteis/farmacologia , Componentes Aéreos da Planta/química , Folhas de Planta/química , Óleos Vegetais/farmacologia , Arábia Saudita , Staphylococcus aureus/efeitos dos fármacos
20.
Anal Biochem ; 622: 114167, 2021 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-33722580

RESUMO

A novel microwave-assisted (MA) headspace solid-phase extraction (HS-SPE) based on polydimethylsiloxane (PDMS)/ZIF-8@GO sponge was developed for the simultaneous extraction and determination of essential oil constituents in lavender. The PDMS/ZIF-8@GO sponge with a high surface area and excellent adsorption capacity was successfully applied in the HS-SPE process. Microwave-assisted coupled with PDMS/ZIF-8@GO sponge headspace solid-phase extraction followed by GC-MS was proposed. Different experimental parameters were investigated. The optimal conditions were found to be as follows: 2:1 as the quality ratio of ZIF-8 to GO, ZIF-8@GO dosage of 30 mg, microwave power of 600 W, extraction time of 10 min and desorption solvent of n-hexane. This method was successfully applied to the analysis of 11 samples of lavender in different varieties. A total of 52 compounds were identified by the proposed method. A good linearity was observed from 14 to 800 ng with a correlation coefficient (R2) value of >0.99. Multivariate statistical analysis was used to establish the relationship between the varieties and the volatile components for further discriminant analysis. These results demonstrated that the MA-PDMS/ZIF-8@GO is an efficient, sensitive and small sample consumption method for the determination of the essential oil in dried plant materials.


Assuntos
Dimetilpolisiloxanos/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Lavandula/química , Óleos Voláteis/análise , Óleos Vegetais/análise , Extração em Fase Sólida/métodos , Adsorção , Micro-Ondas , Análise Multivariada , Microextração em Fase Sólida/métodos
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