Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 258
Filtrar
1.
Am J Trop Med Hyg ; 102(6): 1319-1322, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32228792

RESUMO

The six previously reported civilian cases of mucosal leishmaniasis (ML) diagnosed in the United States have all represented imported New World ML. We describe two new patients with ML diagnosed in New York City-a Syrian immigrant with a nasal mass (Leishmania tropica), the first report of Old World ML in the United States, and an American ecologist who worked in Bolivia and had been treated for cutaneous infection 23 years before developing lesions (L. (Viannia) braziliensis) initially of the uvula, soft palate, and posterior pharynx and subsequently the larynx.


Assuntos
Leishmaniose Mucocutânea/epidemiologia , Leishmaniose Mucocutânea/patologia , Idoso , Anfotericina B/uso terapêutico , Antiprotozoários/uso terapêutico , Humanos , Leishmania braziliensis/isolamento & purificação , Leishmaniose Mucocutânea/tratamento farmacológico , Masculino , Pessoa de Meia-Idade , Cidade de Nova Iorque/epidemiologia , Fosforilcolina/análogos & derivados , Fosforilcolina/uso terapêutico
2.
Parasit Vectors ; 13(1): 24, 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31931865

RESUMO

BACKGROUND: Leishmaniasis, considered by the World Health Organization as one of the most important tropical diseases, is endemic in the Mediterranean Basin. The aim of this study was to evaluate epidemiological and clinical characteristics of cutaneous (CL) and mucocutaneous leishmaniasis (MCL) in La Fe University Hospital, Valencia, Spain. The particular focus was on diagnosis techniques and clinical differences according to the immunological status of the patients. METHODS: An eleven-year retrospective observational study of CL and MCL episodes at the hospital was performed. Epidemiological, clinical and therapeutic variables of each case, together with the microbiological and anatomopathological diagnosis, were analyzed. RESULTS: A total of 42 patients were included, 30 of them were male and 28 were immunocompetent. Most of the cases (36/42) were diagnosed in the last 5 years (2013-2017). The incidence of CL and MCL increased from 3.6/100,000 (2006-2012) to 13.58/100,000 (2013-2017). The majority of the patients (37/42) exhibited CL, in 30 cases as single lesions (30/37). Ulcerative lesions were more common in immunosuppressed patients (13/14) than in immunocompetent patients (20/28), (P = 0.2302). The length of lesion presence before diagnosis was 7.36 ± 6.72 months in immunocompetent patients and 8.79 ± 6.9 months in immunosuppressed patients (P = 0.1863). Leishmania DNA detection (92.3%) was the most sensitive diagnostic technique followed by Giemsa stain (65%) and histopathological examination (53.8%). Twelve patients (12/42) had close contact with dogs or were living near to kennels, and 10 of them did not present underlying conditions. Intralesional glucantime (21/42) and liposomal amphotericin B (7/42) were the most common treatments administered in monotherapy. All patients evolved successfully and no relapse was reported. CONCLUSIONS: Some interesting clinical and epidemiological differences were found in our series between immunocompetent and immunosuppressed patients. Future studies can take these results further especially by studying patients with biological therapy. Skin biopsies combining NAAT with histological techniques are the most productive techniques for CL or MCL diagnosis.


Assuntos
Leishmania/efeitos dos fármacos , Leishmaniose Cutânea , Leishmaniose Mucocutânea , Administração Cutânea , Adolescente , Adulto , Idoso , Anfotericina B/administração & dosagem , Criança , Pré-Escolar , Feminino , Humanos , Hospedeiro Imunocomprometido , Leishmania/isolamento & purificação , Leishmaniose Cutânea/diagnóstico , Leishmaniose Cutânea/tratamento farmacológico , Leishmaniose Cutânea/imunologia , Leishmaniose Cutânea/patologia , Leishmaniose Mucocutânea/diagnóstico , Leishmaniose Mucocutânea/tratamento farmacológico , Leishmaniose Mucocutânea/imunologia , Leishmaniose Mucocutânea/patologia , Masculino , Antimoniato de Meglumina/administração & dosagem , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Espanha/epidemiologia , Adulto Jovem
3.
Am J Trop Med Hyg ; 101(5): 1107-1110, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31549620

RESUMO

Mucocutaneous leishmaniasis (MCL) is a rare infection caused by several species within the genus Leishmania. We present a patient with multifocal MCL masquerading as idiopathic midline granulomatous disease, featuring the unusual complication of ocular leishmaniasis, as a result of prolonged immunosuppressive therapy. We review clinical features, diagnosis, and treatment of this syndrome.


Assuntos
Doença Granulomatosa Crônica/diagnóstico , Leishmaniose Mucocutânea/diagnóstico , Leishmaniose Mucocutânea/patologia , Adulto , Anfotericina B/uso terapêutico , Feminino , Doença Granulomatosa Crônica/tratamento farmacológico , Humanos , Imunossupressores , Leishmania/classificação , Leishmania/isolamento & purificação , Leishmaniose Mucocutânea/tratamento farmacológico
4.
Biomedica ; 39(Supl. 2): 58-65, 2019 08 01.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31529834

RESUMO

INTRODUCTION: Mucosal leishmaniasis has a progressive course and can cause deformity and even mutilation in the affected areas. It is endemic in the American continent and it is mainly caused by Leishmania (Viannia) braziliensis. OBJECTIVE: To describe a series of mucosal leishmaniasis cases and the infectious Leishmania species. MATERIALS AND METHODS: We included 50 patients with a clinical diagnosis of mucosal leishmaniasis and parasitological confirmation, and we described their clinical and laboratory results. We performed species typing by PCR-RFLP using the miniexon sequence and hsp70 genes; confirmation was done by sequencing. RESULTS: The median time of disease evolution was 2.9 years (range: 1 month to 16 years). The relevant clinical findings included mucosal infiltration (94%), cutaneous leishmaniasis scar (74%), total loss of the nasal septum (24%), nasal deformity (22%), and mucosal ulceration (38%). The symptoms reported included nasal obstruction (90%), epistaxis (72%), rhinorrhea (72%), dysphonia (28%), dysphagia (18%), and nasal pruritus (34%). The histopathological study revealed a pattern compatible with leishmaniasis in 86% of the biopsies, and amastigotes were identified in 14% of them. The Montenegro skin test was positive in 86% of patients, immunofluorescence in 84%, and culture in 8%. Leishmania (V.) braziliensis was identified in 88% of the samples, L. (V) panamensis in 8%, and L. (V.) guyanensis and L. (L.) amazonensis in 2% respectively. CONCLUSION: In this study, we found a severe nasal disease with destruction and deformity of the nasal septum in 25% of the cases, probably associated with late diagnosis. Leishmania (V.) braziliensis was the predominant species. We described a case of mucosal leishmaniasis in Colombia caused by L. (L.) amazonensis for the first time.


Assuntos
Leishmania braziliensis/isolamento & purificação , Leishmania guyanensis/isolamento & purificação , Leishmaniose Mucocutânea/parasitologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Colômbia/epidemiologia , DNA de Protozoário/genética , Feminino , Genes de Protozoários , Proteínas de Choque Térmico HSP70/genética , Humanos , Leishmania braziliensis/classificação , Leishmania braziliensis/genética , Leishmania guyanensis/classificação , Leishmania guyanensis/genética , Leishmaniose Mucocutânea/complicações , Leishmaniose Mucocutânea/epidemiologia , Leishmaniose Mucocutânea/patologia , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Proteínas de Protozoários/genética , Análise de Sequência de DNA , Pele/parasitologia , Especificidade da Espécie , Adulto Jovem
5.
Am J Trop Med Hyg ; 101(4): 780-788, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31407656

RESUMO

American tegumentary leishmaniasis is an endemic anthropozoonosis undergoing expansion on the American continent. The disease is caused by several Leishmania species and it is manifested as cutaneous and mucocutaneous leishmaniasis. In this study, we evaluate the viability of high-resolution melt polymerase chain reaction (HRM-PCR) analysis to differentiate four closely related Leishmania species as a routine tool for the diagnosis of leishmaniasis. For this purpose, biopsy specimens from cutaneous and mucocutaneous lesions were taken from 132 individuals from endemic and non-endemic areas for leishmaniasis. Each sample was processed for parasitological, histopathological, and molecular analysis. Positive biopsy samples were analyzed by HRM-PCR of a 144-bp heat-shock protein (hsp70) gene fragment, and new cases were confirmed by sequencing. Of the 132 samples analyzed, 36 (27%) were positive for Leishmania spp., of which 86% were from cutaneous lesions and 14% from mucocutaneous lesions. We identified Leishmania (Viannia) braziliensis (84%), Leishmania (Leishmania) infantum (13%), and Leishmania (Leishmania) amazonensis (3%) in cutaneous lesions, and L. (V.) braziliensis (40%), L. (L.) infantum (20%), L. (L.) amazonensis (20%), and Leishmania (Viannia) guyanensis (20%) in mucocutaneous lesions. The main purpose of this research was to report for the first time in Paraguay the presence of L. (L.) amazonensis and L. (V.) guyanensis in patients with cutaneous and mucocutaneous lesions, using the HRM-PCR technique. In addition, we report the presence of additional new cases of L. (L.) infantum in cutaneous lesions.


Assuntos
Leishmania braziliensis/isolamento & purificação , Leishmania guyanensis/isolamento & purificação , Leishmania infantum/isolamento & purificação , Leishmaniose Cutânea/epidemiologia , Leishmaniose Mucocutânea/epidemiologia , Adulto , Idoso , Feminino , Geografia , Humanos , Leishmania braziliensis/genética , Leishmania guyanensis/genética , Leishmania infantum/genética , Leishmaniose Cutânea/parasitologia , Leishmaniose Cutânea/patologia , Leishmaniose Mucocutânea/parasitologia , Leishmaniose Mucocutânea/patologia , Masculino , Pessoa de Meia-Idade , Paraguai/epidemiologia , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNA , Pele/parasitologia , Pele/patologia
8.
PLoS One ; 13(11): e0208208, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30475920

RESUMO

Mucocutaneous leishmaniasis and paracoccidioidomycosis are infectious diseases with similar epidemiological and clinical aspects. Cases of both diseases may manifest similar lesions in the mucosa. Therefore, the determination of distinguishing characteristics for the purpose of differential diagnosis is critical for better management of the diseases. The present study evaluated factors that assist in the differentiation of mucosal lesions between these diseases. This cross-sectional study included data from medical records of 122 cases of mucocutaneous leishmaniasis and 83 cases of paracoccidioidomycosis attended at the university hospital Cassiano Antonio Moraes, located in Vitória, Espírito Santo State, Brazil. Comparison between the diseases included the following variables: sex, age, time of disease evolution, location of the lesion and symptoms. Adults and males were affected by both diseases at higher rates. Lesions in the nasal region (95.1%; p-value = 0.000) and the pharynx (20.5%; p-value = 0.009) and nasal obstruction (34.4%; p-value = 0.000) were associated with leishmaniasis. Paracoccidioidomycosis was associated with lesions in the oral region (90.4%; p-value = 0.000), oral pain (16.9%; p-value = 0.000), and hoarseness (14.5%; p-value = 0.008). In leishmaniasis, lesions in oral regions were not associated with oral pain and were frequently located close to the nasal area. The manifestations cited above could improve the differential diagnosis of leishmaniasis and paracoccidioidomycosis, and thereby potentially aid in the choice of appropriate confirmatory diagnostic testing.


Assuntos
Leishmaniose Mucocutânea/diagnóstico , Leishmaniose Mucocutânea/patologia , Paracoccidioidomicose/diagnóstico , Paracoccidioidomicose/patologia , Adulto , Fatores Etários , Idoso , Brasil/epidemiologia , Estudos Transversais , Diagnóstico Diferencial , Feminino , Humanos , Leishmaniose Mucocutânea/epidemiologia , Masculino , Pessoa de Meia-Idade , Mucosa Bucal/patologia , Mucosa Nasal/patologia , Paracoccidioidomicose/epidemiologia , Faringe/patologia , Fatores Sexuais , Língua/patologia
10.
Ugeskr Laeger ; 180(35)2018 Aug 27.
Artigo em Dinamarquês | MEDLINE | ID: mdl-30152325

RESUMO

In this case report a male 19-year-old Syrian refugee presented with a sore throat. A biopsy from larynx detected Leishmania tropica compatible with leishmaniasis, although L. tropica does not normally cause mucosal leishmaniasis (CL). An immunodeficiency was detected, and the patient was treated for hypogammaglobulinaemia and CL three times, before the symptoms disappeared. Leishmaniasis is a disease, which should be taken into consideration, when refugees present with atypical clinical manifestations, especially in immunosuppressed patients.


Assuntos
Leishmania tropica/isolamento & purificação , Leishmaniose Mucocutânea/diagnóstico , Agamaglobulinemia/diagnóstico , Agamaglobulinemia/tratamento farmacológico , Dinamarca , Humanos , Leishmaniose Mucocutânea/tratamento farmacológico , Leishmaniose Mucocutânea/patologia , Masculino , Refugiados , Síria/etnologia , Adulto Jovem
11.
An Bras Dermatol ; 93(1): 123-125, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29641713

RESUMO

Brazil is a country with a high prevalence of infectious diseases such as leprosy and leishmaniasis. However, coinfection of these diseases is still poorly understood. We report a case of a patient who presented with lepromatous leprosy and cutaneous-mucosal leishmaniasis at the same period. After clinical, laboratory, and histopathological diagnosis, the treatment was introduced and the patient showed important clinical improvement. He was followed in our outpatient clinic. Both pathologies play an important role in the immune system. Depending on the immune response profile of the host, diseases may present themselves in different ways. In this case, the patient showed a divergent immune response for each disease. We hypothesized that this response is specific for each pathogen.


Assuntos
Coinfecção/complicações , Leishmaniose Mucocutânea/complicações , Hanseníase Virchowiana/complicações , Coinfecção/imunologia , Coinfecção/patologia , Humanos , Imunidade Celular/imunologia , Leishmaniose Mucocutânea/imunologia , Leishmaniose Mucocutânea/patologia , Hanseníase Virchowiana/imunologia , Hanseníase Virchowiana/patologia , Masculino , Pessoa de Meia-Idade
13.
Artigo em Inglês | MEDLINE | ID: mdl-29451596

RESUMO

Cutaneous leishmaniasis (CL) is an endemic disease in the Republic of Panama, caused by Leishmania (Viannia) parasites, whose most common clinical manifestation is the presence of ulcerated lesions on the skin. These lesions usually present a chronic inflammatory reaction, sometimes granulomatous, with the presence of lymphocytes, plasma cells and macrophages. This study describes the histopathological characteristics found in the skin lesions of patients with CL caused by Leishmania (V.) panamensis in Panama. We analyzed 49 skin biopsy samples from patients with clinical suspicion of CL, by molecular tests (PCR for subgenus Viannia and HSP-70) and by Hematoxylin-Eosin staining. Samples were characterized at the species level by PCR-HSP-70/RFLP. From the 49 samples studied, 46 (94%) were positive by PCR and were characterized as Leishmania (V.) panamensis. Of these, 48% were positive by Hematoxylin-Eosin staining with alterations being observed both, in the epidermis (85%) and in the dermis (100%) of skin biopsies. The inflammatory infiltrate was characterized according to histopathological patterns: lymphohistiocytic (50%), lymphoplasmacytic (61%) and granulomatous (46%) infiltration, being the combination of these patterns frequently found. The predominant histopathological characteristics observed in CL lesions caused by L. (V.) panamensis in Panama were: an intense inflammatory reaction in the dermis with a combination of lymphohistiocytic, lymphoplasmacytic and granulomatous presentation patterns and the presence of ulcers, acanthosis, exocytosis and spongiosis in the epidermis.


Assuntos
Leishmania guyanensis/isolamento & purificação , Leishmaniose Mucocutânea/patologia , Adulto , Idoso , Biópsia , Feminino , Humanos , Leishmania guyanensis/genética , Leishmaniose Mucocutânea/parasitologia , Masculino , Pessoa de Meia-Idade , Panamá , Adulto Jovem
14.
An. bras. dermatol ; 93(1): 123-125, Jan.-Feb. 2018. graf
Artigo em Inglês | LILACS | ID: biblio-887166

RESUMO

Abstract: Brazil is a country with a high prevalence of infectious diseases such as leprosy and leishmaniasis. However, coinfection of these diseases is still poorly understood. We report a case of a patient who presented with lepromatous leprosy and cutaneous-mucosal leishmaniasis at the same period. After clinical, laboratory, and histopathological diagnosis, the treatment was introduced and the patient showed important clinical improvement. He was followed in our outpatient clinic. Both pathologies play an important role in the immune system. Depending on the immune response profile of the host, diseases may present themselves in different ways. In this case, the patient showed a divergent immune response for each disease. We hypothesized that this response is specific for each pathogen.


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Hanseníase Virchowiana/complicações , Leishmaniose Mucocutânea/complicações , Coinfecção/complicações , Hanseníase Virchowiana/imunologia , Hanseníase Virchowiana/patologia , Leishmaniose Mucocutânea/imunologia , Leishmaniose Mucocutânea/patologia , Coinfecção/imunologia , Coinfecção/patologia , Imunidade Celular/imunologia
17.
J Craniofac Surg ; 28(5): e446-e447, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28538073

RESUMO

The authors present a clinical report of deforming mucocutaneous leishmaniasis of the nose in a native American woman, left untreated for 25 years. The nose was reconstructed using the local tissue displaced as flaps, and using cartilage grafts taken from the nasal septum and the ear shell. To the best of the authors' knowledge, the literature offers just 1 report on a similar patient.


Assuntos
Leishmaniose Mucocutânea/complicações , Leishmaniose Mucocutânea/patologia , Deformidades Adquiridas Nasais/parasitologia , Deformidades Adquiridas Nasais/cirurgia , Rinoplastia/métodos , Idoso de 80 Anos ou mais , Feminino , Humanos , Deformidades Adquiridas Nasais/patologia
18.
Proc Natl Acad Sci U S A ; 114(19): 4987-4992, 2017 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-28439019

RESUMO

The presence of the endogenous Leishmania RNA virus 1 (LRV1) replicating stably within some parasite species has been associated with the development of more severe forms of leishmaniasis and relapses after drug treatment in humans. Here, we show that the disease-exacerbatory role of LRV1 relies on type I IFN (type I IFNs) production by macrophages and signaling in vivo. Moreover, infecting mice with the LRV1-cured Leishmania guyanensis (LgyLRV1- ) strain of parasites followed by type I IFN treatment increased lesion size and parasite burden, quantitatively reproducing the LRV1-bearing (LgyLRV1+ ) infection phenotype. This finding suggested the possibility that exogenous viral infections could likewise increase pathogenicity, which was tested by coinfecting mice with L. guyanensis and lymphocytic choriomeningitis virus (LCMV), or the sand fly-transmitted arbovirus Toscana virus (TOSV). The type I IFN antiviral response increased the pathology of L. guyanensis infection, accompanied by down-regulation of the IFN-γ receptor normally required for antileishmanial control. Further, LCMV coinfection of IFN-γ-deficient mice promoted parasite dissemination to secondary sites, reproducing the LgyLRV1+ metastatic phenotype. Remarkably, LCMV coinfection of mice that had healed from L. guyanensis infection induced reactivation of disease pathology, overriding the protective adaptive immune response. Our findings establish that type I IFN-dependent responses, arising from endogenous viral elements (dsRNA/LRV1), or exogenous coinfection with IFN-inducing viruses, are able to synergize with New World Leishmania parasites in both primary and relapse infections. Thus, viral infections likely represent a significant risk factor along with parasite and host factors, thereby contributing to the pathological spectrum of human leishmaniasis.


Assuntos
Interferon Tipo I/imunologia , Leishmania guyanensis , Leishmaniose Mucocutânea/imunologia , Leishmaniavirus/imunologia , Coriomeningite Linfocítica/imunologia , Vírus da Coriomeningite Linfocítica/imunologia , Febre por Flebótomos/imunologia , Vírus da Febre do Flebótomo Napolitano/imunologia , Animais , Coinfecção , Interferon Tipo I/genética , Leishmania guyanensis/imunologia , Leishmania guyanensis/virologia , Leishmaniose Mucocutânea/genética , Leishmaniose Mucocutânea/patologia , Coriomeningite Linfocítica/genética , Coriomeningite Linfocítica/patologia , Camundongos , Camundongos Knockout , Febre por Flebótomos/genética , Febre por Flebótomos/patologia
19.
J Infect Dev Ctries ; 11(1): 106-110, 2017 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-28141598

RESUMO

Cutaneous leishmaniasis (CL) is endemic in Ethiopia. An unusual clinical form of this disease is leishmaniasis recidivans (LR), a prolonged, relapsing form of cutaneous leishmaniasis resembling tuberculosis of the skin that may persist for many years with a chronic and relapsing course. This rare variant has been shown to be caused by Leishmania tropica species in the Old World and by Leishmania braziliensis, Leishmania amazonensis, Leishmania panamensis, and Leishmania guyanensis in the New World, as reported in various studies. To our knowledge, there are no reports from Ethiopia, and mucocutaneous involvement of LR has not been described to date. This was a retrospective analysis of the patients seen at the Italian Dermatological Center in Mekelle on the Tigrean highlands over a three-year period (2008-2011). Seven patients with typical clinical features of LR were seen. Two of them presented with signs of mucosal involvement. To date, Leishmania aethiopica is shown to be the only species causing CL that is endemic in the Ethiopian highlands. Therefore, it had to be assumed that the lesions in these patients were caused by this species. The aims of this communication are to report, for the first time, the presence of LR, most likely due to Leishmania aethiopica, in Ethiopia, and to report mucosal involvement in this rare clinical form of CL.


Assuntos
Leishmania/isolamento & purificação , Leishmaniose Mucocutânea/diagnóstico , Leishmaniose Mucocutânea/patologia , Adolescente , Adulto , Criança , Pré-Escolar , Países em Desenvolvimento , Etiópia , Feminino , Humanos , Leishmania/classificação , Masculino , Estudos Retrospectivos
20.
Infect Immun ; 85(3)2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-28052994

RESUMO

Infection by Leishmania (Viannia) panamensis, the predominant etiologic agent for cutaneous leishmaniasis in Colombia, is characterized by a chronic mixed inflammatory response. Current treatment options are plagued by toxicity, lengthy treatment regimens, and growing evidence of drug resistance. Immunotherapy, modulating the immune system to mount a protective response, may provide an alternate therapeutic approach. We investigated the ability of the Toll-like receptor 9 (TLR9) ligand CpG to modulate established disease in the L (V) panamensis mouse model. Treatment of established infection with a high dose (50 µg) of CpG ameliorated disease and lowered parasite burden. Interestingly, immediately after treatment there was a significant increase in transforming growth factor ß (TGF-ß) and concomitantly an increase in T regulatory cell (Treg) function. Although a general reduction in cell-mediated immune cytokine and chemokine (gamma interferon [IFN-γ], interleukin 10 [IL-10], IL-13, IL-6, granulocyte-macrophage colony-stimulating factor [GM-CSF], IL-4, and MIP-1α) responses of the treated mice was observed, certain chemokines (RANTES, monocyte chemoattractant protein 1[MCP-1], and IP-10) were increased. Further, in peripheral blood mononuclear cells (PBMCs) from patients with cutaneous leishmaniasis, CpG treatment similarly exhibited a dose-response effect on the production of IFN-γ, IL-17, IL-10, and IL-13, with reductions observed at higher doses. To further understand the underlying mechanisms and cell populations driving the CpG mediated response, we examined the ex vivo dose effects mediated by the TLR9+ cell populations (dendritic cells, macrophages, and B cells) found to accumulate labeled CpG in vivo Notably, B cells altered the production of IL-17, IL-13, and IFN-γ, supporting a role for B cells functioning as antigen-presenting cells (APCs) and/or regulatory cells during infection. Interestingly, B cells have been previously demonstrated as a primary type of APC in patients infected with L (V) panamensis and thus may be useful targets of immunotherapy. Collectively, our results show that CpG-induced immune regulation leads to a dampening of the host immune response and healing in the mouse model, and it may provide an alternate approach to treatment of cutaneous leishmaniasis caused by L (V) panamensis.


Assuntos
Leishmania guyanensis/imunologia , Leishmaniose Mucocutânea/imunologia , Leishmaniose Mucocutânea/metabolismo , Oligodesoxirribonucleotídeos/metabolismo , Receptor Toll-Like 9/metabolismo , Adulto , Animais , Doença Crônica , Citocinas/metabolismo , Feminino , Humanos , Imunomodulação , Leishmaniose Mucocutânea/parasitologia , Leishmaniose Mucocutânea/patologia , Ligantes , Masculino , Camundongos , Pessoa de Meia-Idade , Carga Parasitária , Subpopulações de Linfócitos T/imunologia , Subpopulações de Linfócitos T/metabolismo , Adulto Jovem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA