Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 6.386
Filtrar
1.
Biochem Med (Zagreb) ; 30(1): 010701, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31839721

RESUMO

Introduction: Chronic obstructive pulmonary disease (COPD) is a complex inflammatory condition that can affect haemostasis. This study aimed to determine differences in platelet-related parameters between controls and COPD subjects. The hypothesis was that platelet indices are disturbed in COPD patients, and this would be accompanied by increased C-reactive protein (CRP), fibrinogen (Fbg) and white blood cells (WBC). Therefore, platelet count (Plt), platelet-related parameters - mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (Pct), their ratios (MPV/Plt, MPV/Pct, PDW/Plt, PDW/Pct), platelet to lymphocyte ratio (PLR), Plt index as well as CRP, Fbg and WBC were assessed. Materials and methods: Study included 109 patients with stable COPD and 95 control subjects, recruited at Clinical Department for Lung Diseases Jordanovac, University Hospital Centre Zagreb (Zagreb, Croatia). Complete blood count was performed on Sysmex XN-1000, CRP on Cobas c501, and Fbg on BCS XP analyser. Data were analysed with MedCalc statistical software. Results: Platelet (P = 0.007) and PLR (P = 0.006) were increased, while other platelet indices were decreased in COPD patients compared to controls. Combined model that included PLR, PDW and WBC showed great diagnostic performances, and correctly classified 75% of cases with an AUC of 0.845 (0.788 - 0.892), P < 0.001. Comorbidities (cardiovascular or metabolic diseases) had no effect on investigated parameters, while inhaled corticosteroids/long-acting ß2-agonists (ICS/LABA) therapy increased MPV and PDW values in COPD patients. Conclusion: Platelet indices were altered in COPD patients and they could be valuable as diagnostic markers of COPD development, especially if combined with already known inflammatory markers.


Assuntos
Biomarcadores/sangue , Plaquetas/citologia , Doença Pulmonar Obstrutiva Crônica/patologia , Corticosteroides/uso terapêutico , Agonistas de Receptores Adrenérgicos beta 2/uso terapêutico , Idoso , Idoso de 80 Anos ou mais , Plaquetas/metabolismo , Doenças Cardiovasculares/complicações , Doenças Cardiovasculares/patologia , Estudos de Casos e Controles , Feminino , Volume Expiratório Forçado , Humanos , Leucócitos/citologia , Modelos Logísticos , Linfócitos/citologia , Masculino , Doenças Metabólicas/complicações , Doenças Metabólicas/patologia , Pessoa de Meia-Idade , Doença Pulmonar Obstrutiva Crônica/tratamento farmacológico
2.
Chemosphere ; 238: 124863, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31551201

RESUMO

Peripheral blood leukocyte telomere length in omethoate-exposed workers is related to environmental exposure and single nucleotide polymorphisms (SNPs) in genes including p21, GSTM1, miR-145, etc. However, the roles of SNPs in tankyrase (TNKS) gene in telomere length are still unknown. The aim of this study was to explore the association between SNPs in TNKS gene and telomere length in omethoate-exposed workers. Telomere length in peripheral blood leukocyte DNA from 180 omethoate-exposed workers and 115 healthy controls was measured using Real-time quantitative polymerase chain reaction (PCR). Genotyping of the selected functional and susceptible SNPs was performed by the flight mass spectrometry based on PCR and single-base extension. The analysis of covariance was performed to find effects of SNPs on telomere length. Generalized linear models were used to analyze the environment, gene, and interaction on telomere length. The results showed that telomere length in the CG + CC genotypes in rs1055328 in TNKS gene was significantly longer than that in the wild homozygous GG genotype both in exposure group (P = 0.017) and in control group (P = 0.038) after adjusting the covariates. The variables kept in the generalized linear models included omethoate-exposure (ß = 0.580, P = 0.001) and rs1055328 (CG + CC) in TNKS gene (ß = 0.339, P = 0.002). The study suggests that the prolongation of telomere length is associated with omethoate-exposure and the CG + CC genotypes in rs1055328 in TNKS gene.


Assuntos
Dimetoato/análogos & derivados , Exposição Ocupacional/efeitos adversos , Tanquirases/genética , Homeostase do Telômero/efeitos dos fármacos , Telômero/fisiologia , Adulto , Inibidor de Quinase Dependente de Ciclina p21/genética , DNA/genética , Dano ao DNA/efeitos dos fármacos , Dimetoato/toxicidade , Feminino , Genótipo , Glutationa Transferase/genética , Humanos , Leucócitos/citologia , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Telômero/genética
3.
Rev Esp Salud Publica ; 932019 Sep 20.
Artigo em Espanhol | MEDLINE | ID: mdl-31537778

RESUMO

BACKGROUND: In the scientific literature, the impacts of plateletpheresis on leukogram parameters are not clear, with a high divergence in the studies that have evaluated changes in leukocytes. The objective of this paper was to meta-analyze the effect of plateletpheresis on the leukocyte count, based on studies published between 1980-2018. METHODS: Systematic review with meta-analysis of random effects for the difference of means. The phases of the PRISMA guide were applied with 132 search strategies in Pubmed, Scielo, Science direct and Scopus. Reproducibility and evaluation of methodological quality were guaranteed. Heterogeneity was evaluated with Galbraith and Dersimonian and Laird's, publication bias with Funnel Plot and Begg; sensitivity analysis, accumulated meta-analysis and Forest Plot were carried out. RESULTS: Nineteen studies were included with 2,358 donors, mostly from India, United States, Turkey, Germany and Austria. A mean difference of -0,80 x109/L (IC95%= -1,96; 0,36x109/L) between the predonation value and the value immediately after donation was founded; no publication bias was found and the conclusion presented good sensitivity since it does not vary with the elimination of studies in successive phases. CONCLUSIONS: The donation of platelets by apheresis does not affect the leukocyte count in the donors, in the last century there were reports of reductions in this parameter, explained by the blood loss in the cases used for the procedure and by effects produced in the blood cells by the biomaterials; however, at present the high safety of plateletpheresis for the white blood cell count is evidenced.


Assuntos
Contagem de Células Sanguíneas , Leucócitos/citologia , Contagem de Plaquetas , Plaquetoferese/métodos , Áustria , Materiais Biocompatíveis , Doadores de Sangue , Alemanha , Humanos , Índia , Reprodutibilidade dos Testes , Espanha/epidemiologia , Turquia , Estados Unidos
4.
Nat Rev Cancer ; 19(10): 587-602, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31492927

RESUMO

The 2018 Nobel Prize in Physiology or Medicine was awarded to pioneers in the field of cancer immunotherapy, as the utility of leveraging a patient's coordinated and adaptive immune system to fight the patient's unique tumour has now been validated robustly in the clinic. Still, the proportion of patients who respond to immunotherapy remains modest (~15% objective response rate across indications), as tumours have multiple means of immune evasion. The immune system is spatiotemporally controlled, so therapies that influence the immune system should be spatiotemporally controlled as well, in order to maximize the therapeutic index. Nanoparticles and biomaterials enable one to program the location, pharmacokinetics and co-delivery of immunomodulatory compounds, eliciting responses that cannot be achieved upon administration of such compounds in solution. The convergence of cancer immunotherapy, nanotechnology, bioengineering and drug delivery is opportune, as each of these fields has matured independently to the point that it can now be used to complement the others substantively and rationally, rather than modestly and empirically. As a result, unmet needs increasingly can be addressed with deductive intention. This Review explores how nanotechnology and related approaches are being applied to augmenting both endogenous leukocytes and adoptively transferred ones by informing specificity, influencing localization and improving function.


Assuntos
Imunoterapia/métodos , Nanomedicina/métodos , Neoplasias/imunologia , Neoplasias/terapia , Transferência Adotiva , Animais , Materiais Biocompatíveis/química , Sistemas de Liberação de Medicamentos , Humanos , Sistema Imunitário , Fatores Imunológicos/metabolismo , Leucócitos/citologia , Nanopartículas/química , Ácidos Nucleicos/metabolismo , Linfócitos T/citologia
5.
Ann Biol Clin (Paris) ; 77(4): 422-428, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31418703

RESUMO

Despite the continuing improvement of automated blood cell counters, confirmation by blood smear examination remains the gold standard in case of anomalies. With a constant goal of standardisation, different experts committees (e.g. the French-speaking cellular hematology group (Groupe francophone d'hématologie cellulaire, GFHC and the ISLH International society for laboratory hematology) recently published criteria for microscopic analysis of blood smears. Cornet et al. evaluated the application of those criteria and propose to suppress any review for 72 hours when a "Blast/Abn lymph" flag is triggered for a sample with no abnormal cell on the microscopic review. The aims of our study were to retrospectively evaluate whether this 72-hour rule adequately operates and whether it is possible to extend the arbitrary 72-hour timeframe to 96h and 144h. To achieve this goal, 40,688 blood samples were collected from three French-speaking hospitals. 1,548 samples presented an isolated "Blast/Abn lymph" flag. Only 221 samples presented the application of the 72-hour rule at least once for our study period. We were able to extend this rule to 144 hours for 10 samples of them. All blood smears for which the rule was applied were verified and there was no abnormal cell on smears at 72 and 144 hours. In conclusion, the 72-hour rule derived from the GFHC's criteria is secure and reduces the slide review rate and thus the production costs and the turnaround time of hemogram results. Further investigations could confirm that its extension to 144 hours is also adequate.


Assuntos
Contagem de Células Sanguíneas , Hematologia/instrumentação , Hematologia/normas , Guias de Prática Clínica como Assunto , Fluxo de Trabalho , Automação Laboratorial/instrumentação , Automação Laboratorial/métodos , Automação Laboratorial/normas , Bélgica , Contagem de Células Sanguíneas/instrumentação , Contagem de Células Sanguíneas/métodos , Contagem de Células Sanguíneas/normas , Coleta de Amostras Sanguíneas/normas , Citodiagnóstico/instrumentação , Citodiagnóstico/métodos , Citodiagnóstico/normas , Reações Falso-Positivas , França , Testes Hematológicos/instrumentação , Testes Hematológicos/métodos , Testes Hematológicos/normas , Hematologia/métodos , Humanos , Ensaio de Proficiência Laboratorial , Contagem de Leucócitos/instrumentação , Contagem de Leucócitos/métodos , Contagem de Leucócitos/normas , Leucócitos/citologia , Linfócitos/citologia , Fase Pré-Analítica/normas , Estudos Retrospectivos , Sensibilidade e Especificidade , Fatores de Tempo
6.
Adv Exp Med Biol ; 1165: 381-406, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31399975

RESUMO

Renal inflammation is the initial, healthy response to renal injury. However, prolonged inflammation promotes the fibrosis process, which leads to chronic pathology and eventually end-stage kidney disease. There are two major sources of inflammatory cells: first, bone marrow-derived leukocytes that include neutrophils, macrophages, fibrocytes and mast cells, and second, locally activated kidney cells such as mesangial cells, podocytes, tubular epithelial cells, endothelial cells and fibroblasts. These activated cells produce many profibrotic cytokines and growth factors that cause accumulation and activation of myofibroblasts, and enhance the production of the extracellular matrix. In particular, activated macrophages are key mediators that drive acute inflammation into chronic kidney disease. They produce large amounts of profibrotic factors and modify the microenvironment via a paracrine effect, and they also transdifferentiate to myofibroblasts directly, although the origin of myofibroblasts in the fibrosing kidney remains controversial. Collectively, understanding inflammatory cell functions and mechanisms during renal fibrosis is paramount to improving diagnosis and treatment of chronic kidney disease.


Assuntos
Mediadores da Inflamação/fisiologia , Nefropatias/fisiopatologia , Rim/patologia , Transdiferenciação Celular , Fibrose , Humanos , Leucócitos/citologia , Macrófagos/citologia , Miofibroblastos/citologia
7.
Nat Commun ; 10(1): 3417, 2019 07 31.
Artigo em Inglês | MEDLINE | ID: mdl-31366909

RESUMO

High costs and technical limitations of cell sorting and single-cell techniques currently restrict the collection of large-scale, cell-type-specific DNA methylation data. This, in turn, impedes our ability to tackle key biological questions that pertain to variation within a population, such as identification of disease-associated genes at a cell-type-specific resolution. Here, we show mathematically and empirically that cell-type-specific methylation levels of an individual can be learned from its tissue-level bulk data, conceptually emulating the case where the individual has been profiled with a single-cell resolution and then signals were aggregated in each cell population separately. Provided with this unprecedented way to perform powerful large-scale epigenetic studies with cell-type-specific resolution, we revisit previous studies with tissue-level bulk methylation and reveal novel associations with leukocyte composition in blood and with rheumatoid arthritis. For the latter, we further show consistency with validation data collected from sorted leukocyte sub-types.


Assuntos
Separação Celular/métodos , Biologia Computacional/métodos , Metilação de DNA/genética , Epigênese Genética/genética , Análise de Célula Única/métodos , Artrite Reumatoide/sangue , Ilhas de CpG/genética , Humanos , Contagem de Leucócitos , Leucócitos/classificação , Leucócitos/citologia
8.
Talanta ; 204: 731-738, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357358

RESUMO

Blood is a routinely tested biological fluid for diagnosis and monitoring of diseases as many diseases would trigger a change in white blood cell count. Thus, several methods have been established to isolate or enrich white blood cells from patient blood samples for such analyses. One method of preparing an enriched white blood cell sample is through the selective lysis of red blood cells by hypotonic shock and restoration of osmolarity to maintain viability of target white blood cells. An inherent problem with this approach is the loss of target cells during sample handling. We report a two-stage separation system that can perform lysis and restoration of osmolarity of blood on-chip and direct the resultant sample to the second step of the analysis. Hence, there is no loss of sample. The post-lysis makeup features a protein-rich buffer to help stabilize cells. As proof of concept, we spiked HL-60 cells into a whole blood and a pre-lysed blood sample and compared capture metrics of each method using a downstream affinity separation. The capture efficiency of the whole blood sample ranged between 40 and 80% using <7 µL of sample compared to 10-52% from 60 µL of pre-lysed blood required for similar analysis. In addition, both pre-lysed and whole blood samples showed no significant difference in purity and viability. This two-stage separation system has demonstrated the capacity to replace centrifugation and wash steps required for the preparation of lysed blood, for white blood cell analyses.


Assuntos
Separação Celular/métodos , Dispositivos Lab-On-A-Chip , Leucócitos/citologia , Separação Celular/instrumentação , Eritrócitos/metabolismo , Células HL-60 , Hemólise , Humanos , Concentração Osmolar , Estudo de Prova de Conceito
9.
Comput Methods Programs Biomed ; 173: 1-14, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31046984

RESUMO

BACKGROUND AND OBJECTIVE: Leukaemia is a disease found worldwide; it is a type of cancer that originates in the bone marrow and is characterised by an abnormal proliferation of white blood cells (leukocytes). In order to correctly identify this abnormality, haematologists examine blood smears from patients. A diagnosis obtained by this method may be influenced by factors such as the experience and level of fatigue of the haematologist, resulting in non-standard reports and even errors. In the literature, several methods have been proposed that involve algorithms to diagnose this disease. However, no reviews or surveys have been conducted. This paper therefore presents an empirical investigation of computational methods focusing on the segmentation of leukocytes. METHODS: In our study, 15 segmentation methods were evaluated using five public image databases: ALL-IDB2, BloodSeg, Leukocytes, JTSC Database and CellaVision. Following the standard methodology for literature evaluation, we conducted a pixel-level segmentation evaluation by comparing the segmented image with its corresponding ground truth. In order to identify the strengths and weaknesses of these methods, we performed an evaluation using six evaluation metrics: accuracy, specificity, precision, recall, kappa, Dice, and true positive rate. RESULTS: The segmentation algorithms performed significantly differently for different image databases, and for each database, a different algorithm achieved the best results. Moreover, the two best methods achieved average accuracy values higher than 97%, with an excellent kappa index. Also, the average Dice index indicated that the similarity between the segmented leukocyte and its ground truth was higher than 0.85 for these two methods This result confirms the high level of similarity between these images but does not guarantee that a method has segmented all leukocyte nuclei. We also found that the method that performed best segmented only 58.44% of all leukocytes. CONCLUSIONS: Of the techniques used to segment leukocytes, we note that clustering algorithms, the Otsu threshold, simple arithmetic operations and region growing are the approaches most widely used for this purpose. However, these computational methods have not yet overcome all the challenges posed by this problem.


Assuntos
Núcleo Celular/metabolismo , Leucócitos/citologia , Algoritmos , Análise por Conglomerados , Cor , Técnicas Citológicas , Bases de Dados Factuais , Aprendizado Profundo , Reações Falso-Positivas , Humanos , Processamento de Imagem Assistida por Computador/métodos , Informática Médica/métodos , Modelos Teóricos
10.
Fish Shellfish Immunol ; 90: 376-384, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31048039

RESUMO

The grass carp (Ctenopharyngodon idella), blunt snout bream (Megalobrama amblycephala) and yellow catfish (Pelteobagrus fulvidraco) are economically important fishes in China. Fish hematological features, especially the type and number of peripheral blood cells, are crucial for the evaluation of fish health and the diagnosis of fish diseases. Since the automatic blood cell count equipment for human is not suitable for fishes, the manual method is critical in the quantification of fish blood cells. To make sense of the comparison and interpretation of the blood cell count studies in different articles, the standardization of blood cell classification is necessary. In this study, erythrocytes (red blood cell, RBC), thrombocytes (TC) and leucocytes (i.e. white blood cells, WBC, including lymphocytes, neutrophils and monocytes) were well distinguished in blood smears with Giemsa staining and confirmed by transmission electron microscopy. RBC, TC and WBC were directly counted with an improved Neubauer counting chamber in a modified diluting solution. The differential leucocyte count (DLC) was carried out in blood smears. In view of the labeling characteristics of peroxidase (PO) positivity in neutrophils and non-specific esterase (α-ANAE) positivity in monocytes, PO positive cell percentage and α-ANAE positive cell percentage were also determined in cytochemistry staining smears. No difference was found for the percentages of neutrophils and monocytes between Giemsa staining and cytochemistry staining. The standardized classification, normal count ranges and sizes of the peripheral blood cells by the present systemic studies will provide useful references for monitoring the health status of grass carp, blunt snout bream and yellow catfish.


Assuntos
Contagem de Células Sanguíneas/veterinária , Carpas/sangue , Peixes-Gato/sangue , Cyprinidae/sangue , Animais , Corantes Azur , Plaquetas/citologia , China , Eritrócitos/citologia , Leucócitos/citologia , Microscopia Eletrônica de Transmissão/veterinária , Valores de Referência
11.
Wiad Lek ; 72(3): 401-404, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31050988

RESUMO

OBJECTIVE: Introduction: Taking into account the patho-immune mechanisms of formation of inflammatory process in periodontium, there is a necessity for in-depth study of the pathogenesis of periodontal diseases from the position of changes in the reactivity of the organism. The aim: Carrying out a comparative estimation of phagocytic activity of leukocytes of animals with inflammation in periodontium with altered reactivity. PATIENTS AND METHODS: Materials and methods: Experiments were performed on 30 white rats: Group I - 10 white rats with hypoergic reaction; Group ІІ - 10 white rats with hyperergic reaction; Group III - 10 white rats with normergic reaction - control group. Slaughter and blood sampling under thiopental anesthesia was performed 7 days after the beginning of the experiment. The percentage of phagocytic leukocytes - phagocytic index, phagocytic number were determined and the index of phagocytic activity was calculated. RESULTS: Results: The phagocytic index (Fi,%) decreased by 2,09 times (p <0,05) at hypoergic, it increased by 1,37 times (p <0,05) at hyperergic; the index of phagocytic activity (IFA) decreased by 1.96 times at hypoergic (p <0.05); growth was 1.94 times (p <0.05) in the hyperergic group of animals. In both experimental groups, the phagocyte number (Fu) increased by 6.25% and 41.7%, with hypoergic and hyperergic, respectively. CONCLUSION: Conclusions: Different directions of changes of the phagocytic activity were observed: increase of these parameters at hyperergic and decrease at hypoergic. An increase in phagocyte number was likely to indicate some autonomy of the process of phagocytosis and independence from the reactivity of the organism.


Assuntos
Periodontite , Animais , Inflamação , Leucócitos/citologia , Periodontite/terapia , Periodonto , Fagocitose/imunologia , Fagocitose/fisiologia , Ratos
12.
Chem Biol Interact ; 308: 20-44, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31067438

RESUMO

Ischemic heart disease (IHD) is a major cause of cardiovascular morbidity and mortality worldwide, which is characterized by an imbalance between cardiac oxygen supply and demand predominantly due to obstruction of coronary arteries. Activation of the innate immune system and the consequent inflammatory response plays a role in the pathogenesis of IHD. Where an excessive inflammatory response may contribute to adverse cardiac remodeling and fibrosis, making inflammation an important therapeutic target for improving outcomes of IHD. While there are many discrepancies in the literature, evidence from both bench and clinical research demonstrate important effects of n-3 polyunsaturated fatty acids (n-3 PUFA), eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA), toward IHD. N-3 PUFAs, and their metabolites, have been demonstrated to modulate various components of the immune system, including regulation of chemokines and cytokines, leukocyte chemotaxis and inflammasome formation. In this article, we provide an overview of the role the innate immune system has in IHD and focus on the immunomodulatory effects of n-3 PUFAs and their biologically active metabolites.


Assuntos
Cardiotônicos/uso terapêutico , Ácidos Graxos Ômega-3/uso terapêutico , Imunidade Inata , Isquemia Miocárdica/tratamento farmacológico , Alarminas/metabolismo , Cardiotônicos/farmacologia , Membrana Celular/química , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Ciclo-Oxigenase 1/metabolismo , Ácidos Graxos Ômega-3/metabolismo , Ácidos Graxos Ômega-3/farmacologia , Humanos , Imunidade Inata/efeitos dos fármacos , Leucócitos/citologia , Leucócitos/efeitos dos fármacos , Leucócitos/metabolismo , Isquemia Miocárdica/imunologia , Isquemia Miocárdica/patologia , Proteína-Lisina 6-Oxidase/metabolismo
13.
Anesth Analg ; 128(6): 1344-1353, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31094810

RESUMO

BACKGROUND: Perioperative major adverse cardiovascular and cerebrovascular events (MACCEs) are incompletely understood, and risk prediction is imprecise. Atherogenic leukocytes are crucial in cardiovascular events. However, it is unclear if surgical interventions affect leukocyte counts or activation status. Therefore, we investigated whether noncardiac surgery in patients with elevated cardiovascular risk is associated with changes in atherogenic leukocyte subsets and if these changes are related to perioperative MACCEs. METHODS: We enrolled 40 patients in this single-center prospective observational cohort study. Total leukocytes and subpopulations, including classical, intermediate, and nonclassical monocytes and natural killer and regulatory T cells, were quantified before surgery, at 2 and 6 hours after skin incision, and at postoperative days 1 and 2 (POD1+2). The monocyte activation marker presepsin (sCD14-ST) was measured post hoc to determine differentiation of classical to nonclassical monocytes. We evaluated presepsin for prediction of the composite primary end point MACCE (cardiovascular death, myocardial infarction, myocardial ischemia, and stroke) at 30 days. Its additive value to risk assessment based on high-sensitive cardiac troponin T and N-terminal probrain natriuretic peptide (NT-proBNP) was analyzed. RESULTS: We evaluated 38 patients, of whom 5 (13%) reached MACCE. In the entire cohort, classical monocytes continuously increased and peaked at POD1 (0.35 [0.23-0.43] cells per nanoliter blood [nL] vs 0.45 [0.31-0.66] cells·nL, preoperative [pre-OP] vs POD1, P = .002). Intermediate monocytes doubled by POD1 (0.017 [0.013-0.021] vs 0.036 [0.022-0.043] cells·nL, pre-OP versus POD1, P = .0003). Nonclassical monocytes decreased (0.022 [0.012-0.032] vs 0.012 [0.005-0.023] cells·nL, pre-OP vs 6 hours, P = .003). In our patient population, we did not detect changes in any of the other predefined leukocyte subsets investigated. In patients experiencing a MACCE, classical monocyte expansion was reduced (0.081 [-0.16 to 0.081] cells·nL vs 0.179 [0.081 to 0.292] cells·nL, MACCE versus non-MACCE, P = .016). Patients in the event group presented with elevated pre-OP presepsin (1528 [406-1897] pg·mL vs 123 [82.2-174] pg·mL, MACCE versus non-MACCE, P = .0001). Presepsin was associated with MACCE (area under the curve = 0.964, [0.846-0.998], P = .001). Presepsin above the calculated threshold >184 pg·mL was superior to high-sensitive cardiac troponin T for improvement of NT-proBNP-based risk prediction (28 [74%] vs 22 [58%] correctly classified patients, P = .014). CONCLUSIONS: Noncardiac surgery was associated with an increase in atherogenic leukocyte subsets. In a post hoc analysis, elevated pre-OP presepsin was associated with MACCE and improved NT-proBNP-based risk assessment. After validation in an independent data set, a presepsin cutoff of 184 pg·mL might qualify to complement NT-proBNP-based risk prediction, thereby increasing the proportion of correctly identified high-risk patients.


Assuntos
Doenças Cardiovasculares/induzido quimicamente , Transtornos Cerebrovasculares/induzido quimicamente , Leucócitos/citologia , Receptores de Lipopolissacarídeos/metabolismo , Fragmentos de Peptídeos/metabolismo , Complicações Pós-Operatórias/diagnóstico , Idoso , Aterosclerose , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Monócitos/citologia , Peptídeo Natriurético Encefálico/sangue , Fragmentos de Peptídeos/sangue , Complicações Pós-Operatórias/metabolismo , Período Pós-Operatório , Estudos Prospectivos , Medição de Risco , Resultado do Tratamento , Troponina T/sangue
14.
Cells ; 8(5)2019 05 16.
Artigo em Inglês | MEDLINE | ID: mdl-31100911

RESUMO

Telomeres are DNA-tandem repeats situated at the ends of chromosomes and are responsible for genome stabilization. They are eroded by increased cell division, age and oxidative stress with shortened leucocyte telomeres (LTL) being associated with inflammatory disorders, including Type II diabetes. We assessed LTL in 205 participants across glucose tolerance groups at baseline and after three years in the mixed ancestry population of South Africa which have been shown to have high rates of obesity and T2DM. Baseline and follow-up data included glucose tolerance status, anthropometric measurements, lipids, insulin, γ-glutamyl transferase (GGT), cotinine, and HbA1c. Telomere length was measured using the absolute telomere q-PCR method performed on a Bio-Rad MiniOpticon Detector. No significant difference was detected in LTL across glucose tolerance groups at both time points, including in subjects who showed a deterioration of their glucose tolerance status. There was, however, a significant negative correlation between LTL and age which was more pronounced in diabetes (r = -0.18, p = 0.04) and with GGT (r = -0.16, p = 0.027). This longitudinal study has demonstrated that LTL shortening is not evident within three years, nor is it associated with glycaemia. Further studies in a larger sample and over a longer time period is required to confirm these results.


Assuntos
Glicemia/análise , Hiperglicemia/epidemiologia , Leucócitos/citologia , Encurtamento do Telômero/genética , Telômero/genética , Fatores Etários , Idoso , Diabetes Mellitus Tipo 2/genética , Feminino , Seguimentos , Teste de Tolerância a Glucose , Humanos , Insulina/sangue , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Obesidade/genética , África do Sul/epidemiologia , gama-Glutamiltransferase/sangue
15.
Biomed J ; 42(2): 93-98, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31130253

RESUMO

BACKGROUND: Modern automated hematology analyzers provide quantitative data on leukocyte size and structure that may be useful to distinguish reactive from neoplastic cellular proliferations. We compared leukocyte volume, conductivity and scatter (VCS) characteristics of chronic myeloid leukemia (CML), bcr-abl1-positive patients with those of non-neoplastic neutrophilia. MATERIALS AND METHODS: Complete blood counts and VCS data (LH750 hematology analyzers, Beckman Coulter) from 38 newly-diagnosed CML patients, 65 CML on imatinib mesylate therapy, 58 patients with elevated age-specific neutrophil counts due to varied causes, 100 pregnant women and 99 healthy controls were collated and compared. Receiver-operating-characteristic curves, logistic regression models and classification trees were studied for their abilities to distinguish various groups. RESULTS: Untreated CML had higher mean neutrophil volume and mean monocyte volume (MNV and MMV), mean lymphocyte scatter (MLS) and higher standard deviations of the mean neutrophil volume and conductivity (MNV-SD and MNC-SD) over all other groups (p < 0.0001 for all). MNV, MNC-SD and MLS distinguished CML from reactive neutrophilia + pregnancy groups (sensitivities 89.5%, 94.7%, 94.7% and specificities 90.6%, 95.6% and 94.0% respectively). Combination of MNV>163.0 AND MNC-SD>12.69 was 89.5% sensitive and 100% specific for CML. Two algorithmic classification-tree approaches using VCS parameters alone (i.e. without the aid of blood count parameters) correctly separated 100% cases of untreated CML from all others. CONCLUSION: Successful distinction of untreated but not post-imatinib CML patients from subjects who were either normal, pregnant or had reactive neutrophilia by automated analyzer-derived cell-population data opens possibilities for their applications in diagnosing and understanding the pathogenesis of CML.


Assuntos
Leucemia Mielogênica Crônica BCR-ABL Positiva/patologia , Contagem de Leucócitos , Leucócitos/citologia , Linfócitos/citologia , Neutrófilos/citologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Leucemia Mielogênica Crônica BCR-ABL Positiva/diagnóstico , Masculino , Pessoa de Meia-Idade , Sensibilidade e Especificidade , Sepse , Adulto Jovem
16.
Clin Lab ; 65(4)2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30969092

RESUMO

BACKGROUND: The Mindray BC-6800 automated hematology analyzer is an automated hematology analyzer and 5-part leukocyte differential counter for in vitro diagnostic use in clinical laboratories. It is necessary to undergo an evaluation before the instrument is used to test patient samples. METHODS: The performance was evaluated with regards to precision, linearity, carry-over, and method comparison. The flag performances were evaluated and compared with the Sysmex XE-2100 hematology analyzer and manual microscope in the hematology laboratory of a tertiary hospital in China. RESULTS: There was minimal carryover (< 0.05%) and excellent linearity for white blood cells and platelet (PLT) counts (r > 0.999). The BC-6800 displayed very good correlation (r > 0.97) with the XE-2100 for blood cell count and cell differential parameters. In a comparison of 295 leukocyte differential count results analyzed in parallel with manual microscopy, the main flags (immature granulocytes, blasts, abnormal lymphocytes) showed approxi-mately the same sensitivity and specificity on both analyzers (sensitivity > 90%, specificity > 78%). CONCLUSIONS: The BC-6800 showed excellent performance and supplied confidence in flag information for abnormal samples in the routine hematology laboratory.


Assuntos
Química Clínica/instrumentação , Química Clínica/métodos , Hematologia/instrumentação , Hematologia/métodos , Automação , Automação Laboratorial , Contagem de Células Sanguíneas/instrumentação , China , Humanos , Contagem de Leucócitos , Leucócitos/citologia , Modelos Lineares , Microscopia , Contagem de Plaquetas , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
17.
Lipids Health Dis ; 18(1): 80, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30935416

RESUMO

BACKGROUND: Evidence regarding the correlation between lipoproteins and telomere length in US adults is limited. We aimed to investigate whether lipoproteins was associated with telomere length using US National Health and Nutrition Examination Survey (NHANES) database. METHODS: A total of 6468 selected participants were identified in the NHANES Data Base (1999-2002). The independent and dependent variables were lipoproteins and telomere length, respectively. The covariates included demographic data, dietary data, physical examination data, and comorbidities. RESULTS: In fully-adjusted model, we found that 0.1 differences of telomere length were positively associated with HDL-C [0.19 (95% CI 0.07, 0.31)], while the associations between LDL-C [0.19 (95% CI -0.27, 0.65)], TG [- 1.00 (95% CI -2.09, 0.07) and telomere length were not detected. By nonlinearity test, only the relationship between HDL-C and telomere length was nonlinear. The inflection point we got was 1.25. On the left side of the inflection point (telomere length ≤ 1.25), a difference in 0.1 of telomere length was associated with 0.50 difference in HDL-C. CONCLUSION: After adjusting for demographic data, dietary data, physical examination data, and comorbidities, telomere length is not associated with LDL-C and TG, but is positively associated with HDL-C when telomere length is less than 1.25.


Assuntos
Hiperlipoproteinemia Tipo II/epidemiologia , Lipoproteínas/genética , Homeostase do Telômero/genética , Telômero/genética , Adulto , Idoso , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Dieta , Feminino , Humanos , Hiperlipoproteinemia Tipo II/sangue , Hiperlipoproteinemia Tipo II/genética , Hiperlipoproteinemia Tipo II/patologia , Leucócitos/citologia , Leucócitos/metabolismo , Lipoproteínas/sangue , Masculino , Pessoa de Meia-Idade , Inquéritos Nutricionais , Fatores de Risco , Triglicerídeos/sangue
18.
Biomater Sci ; 7(7): 2759-2768, 2019 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-31011742

RESUMO

The nanomaterial-cell interface plays an important role in biodetection and therapy. Several parameters involved in the bio-nano interaction such as size, shape, surface charge, surface functionality and protein corona on the nanomaterials have been studied. Recently, we found that the surface charge of the cancer cell membrane derived from the glycolysis could be a general hallmark for cancer cell targeting and very efficient isolation by tailored nanoparticles. However, to simultaneously achieve high capture efficiency and optimal cell viability, the influence of critical features of nanomaterials, such as surface charge and geometry, must be explored. In this study, we designed and synthesized spherical core-shell magnetic particles and Fe3O4 particle coated graphene oxide nanosheets with a similar surface chemistry, charge and magnetization, but different geometries. Although the two-dimensional (2D) graphene oxide based nanocomposites possessed higher capture efficiency at a low working concentration as compared to the spherical nanocomposites, they also exhibited more obvious cytotoxicity. Different aspects of the mechanism underlying the higher cytotoxicity from the 2D nanomaterials were investigated. The results of this study can guide the design of versatile candidates for the isolation of cancer cells.


Assuntos
Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Nanocompostos , Sobrevivência Celular/efeitos dos fármacos , Óxido Ferroso-Férrico/química , Grafite/química , Células HeLa , Humanos , Leucócitos/citologia , Leucócitos/efeitos dos fármacos , Óxidos/química , Polietilenoimina/química , Propriedades de Superfície
19.
Ann Biol Clin (Paris) ; 77(2): 174-178, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30998198

RESUMO

Nowadays, laboratories have more efficient haematology analyzers. These analyzers have to be used in the most efficient and the most adapted way according to the internal organisation of laboratories and prescribers' expectations. The aim of this study was to evaluate the performance of the blast flag on ADVIA 2120/2120i, and to suggest what to do, depending on the flag intensity, to identify positive samples the most surely and the most rapidly as possible. MATERIALS AND METHODS: Seven hospital laboratories were included in this prospective study, 148 144 samples of hospital patients were tested during this 4 months study. RESULTS: Sensitivity and specificity of the blast flag are respectively 89,04% and 98,97%. A good correlation between the flag level and the blast count on blood smear is noticed. CONCLUSION: This study could be helpful for laboratories using ADVIA 2120/2120i, to adapt their procedures, depending on the level of the flag provided by the analyser.


Assuntos
Alarmes Clínicos , Leucócitos/citologia , Coleta de Amostras Sanguíneas/normas , Alarmes Clínicos/normas , França , Testes Hematológicos/métodos , Testes Hematológicos/normas , Humanos , Laboratórios/normas , Ensaio de Proficiência Laboratorial , Contagem de Leucócitos , Leucócitos/patologia , Limite de Detecção , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
20.
Vet Immunol Immunopathol ; 210: 46-54, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30947979

RESUMO

The blood cell counting methods used for diagnostic and research purposes in turkeys are, up to date, inferior to the techniques established for mammals and chickens. While microscopic counts are time consuming, previous flow cytometric approaches did not cover all blood cell types of interest due to the lack of turkey-specific markers for the different cell populations. Moreover, it is unknown to what extent the different leukocyte populations are affected by host and environmental factors including age, breed of the bird, and housing environment, respectively. In this study we established a whole blood based flow cytometric analysis method for turkeys. This method was used to determine baseline values depending on the age of the birds as well as under consideration of variations between trials and animal room effects. During three trials whole blood samples of B.U.T. 6 female turkeys were collected to analyze different leukocyte concentrations (cells/µl whole blood). In the first trial one group and in the second and third trial two groups with 22 birds/group were housed. Blood samples were collected at days one, 23, 43, 60, and 88 post hatch and concentrations of monocytes, MHC class II-positive, CD4+, and CD8+ lymphocytes, as well as thrombocytes and granulocytes were determined by flow cytometric analysis. Concentrations of all identified populations were not only influenced by the bird's age (p < 0.05), they varied also among trials (p < 0.05) and even for some of the populations between animal rooms within the same trial despite comparable housing and management conditions. Therefore, for the establishment of baseline values for leucocyte concentrations in whole blood effects of age and housing have to be considered. In addition, our data emphasize the importance of the establishment of baseline values for different age groups, as age had the strongest effect on the blood cell numbers in this study.


Assuntos
Citometria de Fluxo , Contagem de Leucócitos/veterinária , Leucócitos/citologia , Fatores Etários , Animais , Feminino , Granulócitos/citologia , Abrigo para Animais , Contagem de Leucócitos/métodos , Monócitos/citologia , Perus
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA