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1.
Waste Manag ; 118: 585-590, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33010689

RESUMO

The present work describes the feasibility of coffee residue extracts as cryoprotective agents in the storage stability of freeze-dried lactic acid bacteria. Coffee residue extracts were extracted from coffee residue, produced after coffee extraction for coffee powder and instant coffee preparation, using an autoclave. Leuconostoc mesenteroides WiKim32 was selected to evaluate the ability of coffee residue extracts to protect bacteria during freeze-dried storage. The storage stability of freeze-dried Leu. mesenteroides WiKim32 with coffee residue extracts was comparable to those with commercial cryoprotective agents. Coffee residue extracts contributed to storage stability immediately after freeze-drying (61.2%) and subsequent storage (48.7%). Our data indicate that the protective effect of the coffee residue extracts is associated with ions, carbohydrates, and phenolic compounds. Coffee residue extracts are feasible materials, which can reduce the storage and distribution costs compared to commercial agents currently available.


Assuntos
Café , Lactobacillales , Liofilização , Expectativa de Vida , Pós
2.
PLoS One ; 15(9): e0229841, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32946470

RESUMO

Starch based gluten-free bread (formulations containing mixture of corn and potato starch with hydrocolloids) are deficient in nutrients and do not contain health promoting compounds. Therefore they could be supplemented with raw materials rich in such components, especially antioxidants. Among them pseudo-cereals, seeds, fruits and vegetables are often applied to this purpose. Potato pulp produced by processing red fleshed (Magenta Love) and purple fleshed (Violetta) varieties could become a new innovative substrate for gluten-free bread enrichment, because of high levels of endogenous polyphenols, namely flavonoids, flavonols, phenolic acids and especially anthocyanins with high antioxidant potential, as well as dietary fiber. Study material consisted of gluten-free bread enriched in the pulp. Dietary fiber, acrylamide content and antioxidant and antiradical potential of the bread were determined. Sensory evaluation included crumb elasticity, porosity and other characteristics, taste and smell. Among all analyzed gluten-free breads, the sample containing 7.5% share of freeze-dried red potato pulp Magenta Love was characterized by high content of phenolic compounds and dietary fiber, pronounced antioxidant activity, low levels of potentially dangerous acrylamide and good physical and sensory characteristics. Therefore such an addition (7.5% Magenta Love) could be recommended for industrial production of gluten-free bread.


Assuntos
Pão , Farinha/análise , Ingredientes de Alimentos/análise , Solanum tuberosum/química , Verduras/química , Acrilamida/análise , Antioxidantes/análise , Doença Celíaca/dietoterapia , Doença Celíaca/imunologia , Fibras na Dieta/análise , Liofilização/métodos , Glutens/imunologia , Humanos , Valor Nutritivo , Polifenóis/análise , Porosidade , Olfato , Amido/isolamento & purificação , Paladar
3.
An Acad Bras Cienc ; 92(2): e20190072, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32785423

RESUMO

To preserve freshness and nutritional quality of fruits and vegetables is required large energy expenditure due to most storage techniques require low temperatures, making the product difficult to transport and store. PURPOSE: To reduce energy expenditure, dehydration processes are being improving by pretreatments and changing frozen stage. METHOD: Alkaline emulsion pretreatment was introduced to freeze-drying method to reduce the drying time of grapes and obtain significant energy savings. RESULTS: Dehydration process for untreated fresh grape samples was 22 hours, using industrial freeze-drying equipment. It was obtained a high quality product with range 3 % to 7% of final humidity, without losing natural characteristics and organoleptic properties of the grapes. CONCLUSIONS: it was found by modifying standard equipment frozen method, using dry ice (CO2) and applied coconut oil alkaline emulsion pretreatment, a 54% energy saving. Even more the dehydration process decreased the microbial load in the fruit. A product with low number of microorganisms may be consumed by patients with low defenses as cereals, nutritional bars, salads, yogurts, etc. Finally, if all enterprises involved in this field take into account these findings and improve constantly their process they will stop emitting several kg of CO2 into the atmosphere.


Assuntos
Vitis , Dessecação , Liofilização
4.
Int J Nanomedicine ; 15: 5361-5376, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32801694

RESUMO

Background and Aim: Polymeric nanoparticles (NPs) have received much attention as promising carrier systems in lung cancer and brain metastases. Methods: Here, for the first time, we investigated the feasibility of using inhaled cholesterol-PEG co-modified poly (n-butyl) cyanoacrylate NPs (CLS-PEG NPs) of docetaxel (DTX) for sustained pulmonary drug delivery in cancer metastasis. Results: Spray-dried or freeze-dried NPs yielded sustained drug release in vitro. In vitro inhalation evaluation data indicated that the inhalation formulation had better inhalability. Compared with intravenous (IV) administration, pharmacokinetic data suggested that the inhalation formulation prolonged plasma concentration of DTX for greater than 24 h and is more quickly and completely absorbed into the rat lung after intratracheal (IT) administration. Furthermore, freeze-dried powders were found to increase the t1/2 and area under curve (AUC) by 2.3 and 6.5 fold compared to the free drug after IT administration, and spray-dried powders were found to increase the t1/2 and AUC by 3.4 and 8.8 fold, respectively. After pulmonary administration of the inhalation formulation, DTX appeared to prolong the pulmonary absorption time. In addition, the inhalation formulation was distributed to the brain in a sustained release manner. Conclusion: These experimental results demonstrated that freeze- and spray-dried powders have the potential for pulmonary sustained release, and they also have the potential to be used as a novel treatment for the delivery of drugs that pass through the air-blood barrier and enter the brain and are efficient carriers for the treatment of brain metastasis.


Assuntos
Antineoplásicos/administração & dosagem , Docetaxel/administração & dosagem , Neoplasias Pulmonares/tratamento farmacológico , Nanopartículas/administração & dosagem , Células A549 , Administração por Inalação , Animais , Encéfalo/efeitos dos fármacos , Colesterol/química , Preparações de Ação Retardada , Docetaxel/farmacocinética , Portadores de Fármacos/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Embucrilato/química , Feminino , Liofilização , Humanos , Pulmão/efeitos dos fármacos , Masculino , Camundongos Endogâmicos C57BL , Nanopartículas/química , Tamanho da Partícula , Polímeros/química , Pós/química , Ratos Wistar , Distribuição Tecidual
5.
Food Chem ; 331: 127352, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32652343

RESUMO

The influence of some additives, including metal ions, antioxidants, enzyme inhibitors and organic solvents, on the storage stability of four organophosphorus pesticides in cucumber samples were investigated. It was found that metal ions, including Al3+, Fe3+, and Co2+, increased the stability of dichlorvos, malathion, and chlorpyrifos. Conversely, Al3+, Fe3+, Fe2+, and Co2+ caused catalytic degradation of diazinon. With the addition of organic solvents (CH2Cl2, CHCl3, CCl4, CH3OH and CH3COCH3), remaining of diazinon residues was higher (16-54%) after storage for seven days. CCl4 was associated with the highest retention of malathion, diazinon, and chlorpyrifos (33%, 48% and 44%, respectively) in samples. SDS also stabilized the pesticides since residues were, again, higher (13-38%) after seven days storage. Furthermore, addition of Al3+ and Fe3+ decreased peroxidase (POD) activity and inhibited degradation of dichlorvos and malathion. After 14 days, lyophilization increased the pesticide residues remaining by 36%, 29%, and 58% for diazinon, malathion and chlorpyrifos, respectively. Overall, the stability of these pesticides during storage is impacted by water content and addition of exogenous substances. This could ensure higher quality of pesticide residue data in samples.


Assuntos
Cucumis sativus/química , Contaminação de Alimentos/análise , Armazenamento de Alimentos , Inseticidas/química , Compostos Organofosforados/química , Alumínio/química , Clorpirifos/análise , Clorpirifos/química , Diazinon/análise , Diazinon/química , Diclorvós/análise , Diclorvós/química , Aditivos Alimentares/química , Liofilização , Inseticidas/análise , Malation/análise , Malation/química , Compostos Organofosforados/análise , Oxirredutases/química , Peroxidases/química , Peroxidases/metabolismo , Resíduos de Praguicidas/análise , Resíduos de Praguicidas/química , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Dodecilsulfato de Sódio/química , Água/química
6.
Food Chem ; 331: 127203, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32574943

RESUMO

Effects of high hydrostatic pressure (HHP) (50, 100, 150, 200 and 250 MPa) pretreatment on water mobility and distribution, drying duration, microstructure, color, cell wall fraction and tissue structure of strawberry slices were investigated. HHP significantly increased water mobility of the strawberry slices, resulting in the reduction of drying duration by 9-24%. As the pretreatment pressure was increased, redness value and anthocyanin content continuously increased, soluble pectin (SBP) content increased and then decreased, while the contents of protopectin (PTP) and cellulose decreased. After the HHP pretreatment, chromoplasts and moisture was distributed more uniformly in the strawberry slices. Microscopy images showed the formation of microscopic holes or channels in the matrix and the breakdown of tissue structure by HHP. Results suggested HHP pretreatment disrupted the integrity of the fresh strawberry which enhanced the drying efficiency and migration of the chromoplasts during the vacuum-freeze drying process.


Assuntos
Fragaria/química , Liofilização , Antocianinas/química , Antocianinas/metabolismo , Celulose/química , Cor , Fragaria/metabolismo , Pressão Hidrostática , Espectroscopia de Ressonância Magnética , Pectinas/química , Pectinas/metabolismo , Água/química
7.
J Environ Sci Health B ; 55(9): 794-802, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32586204

RESUMO

Herbicide use, mainly glyphosate, has been intense in worldwide agriculture, including in the Brazilian Amazon region. This study aimed to validate a method for determining glyphosate and its degradation product, AMPA, and glufosinate by HPLC-FL in 58 water samples collected at the Santarém plateau region (Planalto Santareno), in the western of Pará state, Brazil. The method involves filtration and direct injection in the HPLC-FL for AMPA analysis, or previous concentration (10×) by lyophilization for glufosinate and glyphosate analysis. Analytes were oxidized and complexed with o-phthalaldehyde and 2-mercaptoethanol in a post-column reaction before fluorescence detection. LOQs for AMPA, glyphosate and glufosinate were established at 0.5, 0.2 and 0.3 µg L-1, respectively. A total of 58 samples were collected. Glyphosate and glufosinate were not detected in any of the 30 surface water samples collected in 2015 (

Assuntos
Aminobutiratos/análise , Cromatografia Líquida de Alta Pressão/métodos , Glicina/análogos & derivados , Poluentes Químicos da Água/análise , Água/química , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiônico/análise , Brasil , Monitoramento Ambiental , Fluorescência , Liofilização , Glicina/análise , Herbicidas/análise , Limite de Detecção , Reprodutibilidade dos Testes
8.
Clin Biochem ; 84: 73-78, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32592724

RESUMO

OBJECTIVES: A novel coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) emerged in late 2019, causing an outbreak of pneumonia [coronavirus disease 2019 (COVID-19)] globally. Although the use of ready-made reaction mixes can enable more rapid PCR-based diagnosis of COVID-19, the need to transport and store these mixes at low temperatures presents challenges to already overburdened logistics networks. METHODS: Here, we present an optimized freeze-drying procedure that allows SARS-CoV-2 PCR mixes to be transported and stored at ambient temperatures, without loss of activity. Additive-supplemented PCR mixes were freeze-dried. The residual moisture of the freeze-dried PCR mixes was measured by Karl-Fischer titration. RESULTS: We found that the freeze-dried PCR mixes with ~1.2% residual moisture are optimal for storage, transport, and reconstitution. The sensitivity, specificity, and repeatability of the freeze-dried reagents were similar to those of freshly prepared, wet reagents. The freeze-dried mixes retained activity at room temperature (18 ~ 25 °C) for 28 days, and for 14 and 10 days when stored at 37 °C and 56 °C, respectively. CONCLUSION: The uptake of this approach will ease logistical challenges faced by transport networks and make more cold storage space available at diagnosis and hospital laboratories.


Assuntos
Betacoronavirus/genética , Técnicas de Laboratório Clínico/métodos , Infecções por Coronavirus/diagnóstico , Primers do DNA/química , DNA Viral/análise , Pneumonia Viral/diagnóstico , Reação em Cadeia da Polimerase/métodos , Betacoronavirus/isolamento & purificação , Infecções por Coronavirus/virologia , DNA Viral/genética , Liofilização , Humanos , Pandemias , Pneumonia Viral/virologia , Temperatura
9.
PLoS One ; 15(6): e0234502, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32525915

RESUMO

Preservation of blood plasma in the dried state would facilitate long-term storage and transport at ambient temperatures, without the need of to use liquid nitrogen tanks or freezers. The aim of this study was to investigate the feasibility of dry preservation of human plasma, using sugars as lyoprotectants, and evaluate macromolecular stability of plasma components during storage. Blood plasma from healthy donors was freeze dried using 0-10% glucose, sucrose, or trehalose, and stored at various temperatures. Differential scanning calorimetry was used to measure the glass transition temperatures of freeze-dried samples. Protein aggregation, the overall protein secondary structure, and oxidative damage were studied under different storage conditions. Differential scanning calorimetry measurements showed that plasma freeze-dried with glucose, sucrose and trehalose have glass transition temperatures of respectively 72±3.4°C, 46±11°C, 15±2.4°C. It was found that sugars diminish freeze-drying induced protein aggregation in a dose-dependent manner, and that a 10% (w/v) sugar concentration almost entirely prevents protein aggregation. Protein aggregation after rehydration coincided with relatively high contents of ß-sheet structures in the dried state. Trehalose reduced the rate of protein aggregation during storage at elevated temperatures, and plasma that is freeze- dried plasma with trehalose showed a reduced accumulation of reactive oxygen species and protein oxidation products during storage. In conclusion, freeze-drying plasma with trehalose provides an attractive alternative to traditional cryogenic preservation.


Assuntos
Proteínas Sanguíneas/metabolismo , Plasma/química , Preservação Biológica/métodos , Conservantes Farmacêuticos/química , Trealose/química , Proteínas Sanguíneas/química , Estabilidade de Medicamentos , Armazenamento de Medicamentos , Liofilização , Humanos , Agregados Proteicos , Conformação Proteica em Folha beta , Estabilidade Proteica , Temperatura de Transição , Vitrificação
10.
Food Chem ; 331: 127201, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32562976

RESUMO

Volatile compounds in Chinese jujubes dried by different methods - hot-air (HAD), heat-pump (HPD), infrared radiation (IRD), vacuum (VD), vacuum freeze (VFD) and instant controlled pressure drop (DIC) drying - were analyzed using GC-MS, MOS e-nose, and flash GC e-nose. Acids comprised more than 90% of the aroma compounds in the dried jujubes, of which acetic, butanoic, propanoic, hexanoic, octanoic and decanoic acids were the most common. Jujubes dried using VFD had the highest content of total aroma compounds (1061.6 µg/kg), while DIC-dried jujubes had the most diverse profile (26 species). HPD-, IRD-, HAD- and VD-dried jujubes had similar aroma profiles based on GC-MS and flash GC e-nose results. Although the results of GC-MS, MOS e-nose, and flash GC e-nose were significant different (p < 0.05), their combination could characterize aroma profiles more comprehensively.


Assuntos
Nariz Eletrônico , Cromatografia Gasosa-Espectrometria de Massas/métodos , Compostos Orgânicos Voláteis/análise , Ziziphus/química , Dessecação , Análise de Alimentos/métodos , Liofilização , Odorantes/análise
11.
AAPS PharmSciTech ; 21(5): 163, 2020 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-32488738

RESUMO

The aim of this study was to evaluate a multicomponent complex (MC) between rifampicin (RIF), ß-cyclodextrin (ß-CD), and selected amino acids to enhance the solubility and antibiofilm activity of RIF. After performing phase-solubility studies that demonstrated a considerable increase in the solubility of RIF for the MC, the corresponding solid system was prepared by a freeze-drying method. Characterization of the MC was performed by Fourier transform-infrared spectroscopy, thermal analysis, powder X-ray diffraction, and scanning electron microscopy. Structural analyses evidenced molecular interactions between the components, resulting in a MC with amorphous solid features. Structural studies involving both experimental (i.e., 1H NMR) and theoretical (i.e., molecular modeling) methodologies demonstrated the inclusion of the RIF piperazine ring in the ß-CD cavity. The bioactivity of the MC measured against biofilms of Staphylococcus aureus showed a significant reduction in the metabolic activity of the bacterium. Overall, the studied MC exhibited promising properties for the development of pharmaceutical formulations to treat bacterial infections.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Rifampina/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/química , Varredura Diferencial de Calorimetria , Composição de Medicamentos , Liofilização/métodos , Microscopia Eletrônica de Varredura , Pós , Rifampina/química , Solubilidade , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Difração de Raios X , beta-Ciclodextrinas/química
12.
Food Chem ; 329: 127178, 2020 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-32502746

RESUMO

A betacyanin rich extract was obtained from the flowers of Gomphrena globosa L. by ultrasound-assisted extraction and dried either by lyophilization or spray-drying, was tested as a natural colourant in cookies and compared to a commercial colourant. The extracts were characterized in terms of betacyanin content and antioxidant potential. The effects of the colourants incorporation in the cookies were assessed through proximate composition, soluble sugars, fatty acids, color, texture and microbial load, over a shelf life of 30 days. Considering all the assays and analyzing the results through a 2-way analysis of variance, the cookies incorporated with spray-dried colourant showed the most intense pink coloration while cookies incorporated with lyophilized extract lost less color intensity over time. Thus, betacyanin extracts have potential as pink natural alternatives to synthetic colourants in the food industry.


Assuntos
Amaranthaceae/química , Betacianinas/química , Doces/análise , Corantes/química , Amaranthaceae/metabolismo , Antioxidantes/química , Betacianinas/isolamento & purificação , Dessecação , Flores/química , Flores/metabolismo , Liofilização , Valor Nutritivo , Extratos Vegetais/química , Sonicação
13.
Food Chem ; 330: 127357, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32569943

RESUMO

Attenuated total reflectance Fourier transform spectroscopy (ATR-FTIR) was applied on fresh (NF), freeze-dried (FD) and cell wall materials (AIS) of raw and processed apples. These samples prepared from 36 apple sets and the corresponding 72 purees, issued from different varieties, agricultural practices, storage periods and processing conditions, were used to build models including exploratory analysis, supervised classification and multivariate calibration. Fresh and freeze-dried samples presented similar fingerprint spectral variations due to processing. ATR-FTIR directly on fresh purees satisfactorily predicted textural properties such as particle average size and volume (RPD > 3.0), while freeze-drying improved assessment of chemical (RPD > 3.2) and rheological (RPD > 3.1) parameters using partial least-squares regression. The assessment of texture and macrocomponents of purees can be obtained with a limited sample preparation. For research applications because of a need of sample preparation, changes of cell wall composition during fruit processing could be assessed in relationship with pectin degradation.


Assuntos
Parede Celular/química , Indústria de Processamento de Alimentos/métodos , Malus/química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Calibragem , Análise de Alimentos/métodos , Análise de Alimentos/estatística & dados numéricos , Liofilização , Frutas/química , Análise dos Mínimos Quadrados , Malus/citologia , Tamanho da Partícula , Reologia , Espectroscopia de Infravermelho com Transformada de Fourier/estatística & dados numéricos
14.
Food Chem ; 331: 127341, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32569972

RESUMO

The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides. Twelve compounds were tentatively identified by UHPLC-Q-TOF-MS/MS in CLE and PPE extracts. In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical. The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol. The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation. The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.


Assuntos
Antocianinas/análise , Clitoria/química , Flores/química , Extratos Vegetais/química , Antioxidantes/química , Cromatografia Líquida de Alta Pressão , Corantes de Alimentos/análise , Corantes de Alimentos/química , Liofilização , Concentração de Íons de Hidrogênio , Oligossacarídeos/química , Fenóis/análise , Pigmentação , Extratos Vegetais/análise , Espectrometria de Massas em Tandem , Temperatura
15.
Food Chem ; 331: 127378, 2020 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-32593797

RESUMO

Selenium nanoparticles (Se0NPs) have been well-characterized; however, whether processing affects their physicochemical and functional properties remains unknown. Here, chitosan (low and high molecular weight; CS(L) and CS(H), respectively) was used to stabilize Se0NPs, and the effects of heating (37 â„ƒ, 70 â„ƒ, and 95 â„ƒ), freeze-drying-rehydration, and freeze-thawing on CS-Se0NPs physicochemical stability, Se release, antioxidant capacity, and antibacterial activity were evaluated. The results demonstrated that all treatments could cause CS-Se0NPs aggregation and Se release to varying degrees. Aggregation of CS-Se0NPs decreased their antibacterial activity, while Se release increased their antioxidant capacity with negligible effects on antibacterial activities. None of the CS-Se0NPs could tolerate freeze-thawing. CS(H)-Se0NPs exhibited better rehydration and heating stability than CS(L)-Se0NPs, although "rod-like" triclinic crystalline Se in CS(H)-Se0NPs, produced by 95℃ heating, decreased both antioxidant and antibacterial activities. Thus, these results provide a theoretical basis for the development and suitable application of CS-Se0NPs.


Assuntos
Antibacterianos/farmacologia , Quitosana/química , Nanopartículas/química , Selênio/química , Antibacterianos/química , Antioxidantes/química , Antioxidantes/farmacologia , Liofilização , Peso Molecular , Tamanho da Partícula , Selênio/farmacocinética , Difração de Raios X
16.
Food Chem ; 326: 126849, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32447159

RESUMO

Buffer salts are often added to culture medium to promote bacterial growth. However, we found that buffer salts can improve the freeze-drying survival rate. In this experiment, the mechanisms for the effects of different buffer salts on the survival rate of freeze-dried strains were examined. The results showed that buffer salts had important effects on the freeze-drying survival rate of L. plantarum LIP-1 that were related to changes in fatty acid composition. Different buffer salts affected the expression of fatty acid metabolic genes. A new gene cluster that regulates fatty acid metabolism and synthesis was discovered. Potassium ions in buffer salts upregulated the trkA gene and lysR-type transcription factor, and then upregulated the expression of fatty acid synthesis-related acc and fab family genes. These genes help to extend the fatty acid carbon chain and promote the unsaturated fatty acids content, which improves cell membrane fluidity and improves resistance to freeze-drying.


Assuntos
Lactobacillus plantarum/metabolismo , Proteoma/metabolismo , Sais/farmacologia , Transcriptoma , Ácidos Graxos/metabolismo , Liofilização , Lactobacillus plantarum/efeitos dos fármacos , Fluidez de Membrana
17.
Food Chem ; 328: 127127, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32473492

RESUMO

Poor stability of fish hydrolyzed collagen (HC) hampers its applications, especially as food ingredients. The use of liposome as a vesicle can be a potential means to enhance bioactivities and stability of HC. HC from defatted Asian sea bass skin at different levels (0.25%-2%, w/v) were loaded into liposomes prepared from soy phosphatidylcholine (SPC) with various stabilizers (cholesterol (CHO) or glycerol (GLY)). The highest encapsulation efficiency (EE) was found in SPC-CHO-0.5%HC (P < 0.05) (85.42%), while liposome stabilized with GLY had the highest EE (74.54%) for SPC-GLY-0.25%HC (P < 0.05). After lyophilization, SPC-CHO-0.5%HC had higher EE than SPC-GLY-0.25%HC (P < 0.05). Increasing particle size and decreasing negative surface charge were found for both lyophilized samples. Lyophilized SPC-CHO-0.5%HC exhibited higher stability than lyophilized SPC-GLY-0.25%HC during storage at 25 °C for 28 days. Also, higher antioxidant activities in gastrointestinal track model system was found for SPC-CHO-0.5%HC. Thus, SPC-CHO liposome could be used as a promising carrier of HC.


Assuntos
Antioxidantes/química , Colágeno/química , Lipossomos/química , Lipossomos/farmacologia , Animais , Antioxidantes/farmacologia , Bass , Colesterol/química , Excipientes/química , Armazenamento de Alimentos , Liofilização , Trato Gastrointestinal , Glicerol , Hidrólise , Lecitinas/química , Tamanho da Partícula , Fosfatidilcolinas/química , Pele/química , Temperatura
18.
Food Chem ; 326: 126965, 2020 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-32413755

RESUMO

Natural deep eutectic solvents (NADES) were introduced for the extraction of free seleno-amino acids from lyophilized and powdered milk samples. Different NADES were evaluated, and lactic acid:glucose (LGH) showed the highest selenium recoveries. Selenium analysis was performed by inductively coupled plasma mass spectrometry (ICP MS). Se-NADES analysis in ICP MS was optimized according to the radio frequency power and nebulization gas flow rate. Se-NADES extraction was optimized by an experimental design. LGH dilution, LGH volume, sample quantity, and ultrasound time were factors influencing the extraction. Seleno-amino acids were determined by liquid chromatography-ICP MS. After optimization, the limits of detection obtained were 7.37, 8.63, and 9.64 µg kg-1 for selenocysteine, selenomethionine, and seleno-methyl-selenocysteine, respectively. The NADES-extraction is a green procedure with 2 penalty points in the EcoScale. The method was applied to the analysis of powdered milk, lyophilized Se-fortified sheep milk, and ERM-BD151 skimmed milk powder.


Assuntos
Análise de Alimentos/métodos , Leite/química , Selenocisteína/análogos & derivados , Selenocisteína/análise , Selenometionina/análise , Animais , Fracionamento Químico/métodos , Cromatografia Líquida , Feminino , Alimentos Fortificados/análise , Liofilização , Química Verde , Limite de Detecção , Espectrometria de Massas/métodos , Pós/química , Selênio/análise , Ovinos , Solventes/química
19.
AAPS PharmSciTech ; 21(5): 143, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32424702

RESUMO

This work aims to improve the functionality of Rosmarinus officinalis L. (rosemary) polyphenols by encapsulation in an optimized proliposome formulation. A 23 Box-Wilson central composite design (CCD) was employed to determine lone and interaction effects of composition variables on moisture content (Xp); water activity (Aw); concentration and retention of rosemary polyphenols-rosmarinic acid (ROA), carnosol (CAR), and carnosic acid (CNA); and recovery of spray-dried proliposomes (SDP). Processing conditions which generate proliposomes with optimum physicochemical properties were determined by multi-response analysis (desirability approach). Antioxidant and antifungal activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH•) sequestering and minimum inhibitory concentration (MIC)/minimum fungicidal concentration (MFC) assays, respectively. SDP exhibited high polyphenol retention, ranging from 62.0 to 100.0% w/w, showing dependence on composition variables and polyphenol lipophilicity. SDP recovery ranged from 20.1 to 45.8%, with Xp and Aw of 1.7 ± 0.14-2.5 ± 0.23% w/w and 0.30 ± 0.004-0.47 ± 0.003, respectively, evidencing product with good chemical and microbiological stability. Optimum liposomal composition was determined, namely, lipid concentration (4.26% w/w), lyophilized extract (LE) concentration (4.48% w/w), and drying aid:(lipid+extract) ratio (7.55% w/w) on wet basis. Relative errors between experimental and predicted values for SDP properties showed concurrence for all responses except CAR retention, being 22% lower. SDP showed high antioxidant activity with IC50 of 9.2 ± 0.2 µg/mL, superior to results obtained for LE (10.8 µg/mL) and butylated hydroxytoluene (BHT), a synthetic antioxidant (12.5 µg/mL). MIC and MFC against Candida albicans (ATCC1023) were 312.5 µg/mL and 1250 µg/mL, respectively, a moderate antimicrobial activity for phytochemical-based products. SDP is shown as a veritable tool to encapsulate hydrophilic and lipophilic rosemary polyphenols generating a product with optimal physicochemical and biological properties.


Assuntos
Lipossomos/química , Polifenóis/química , Rosmarinus/química , Antifúngicos/farmacologia , Antioxidantes/farmacologia , Candida albicans/efeitos dos fármacos , Cápsulas , Dessecação , Composição de Medicamentos , Liofilização , Humanos , Lipídeos/química , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Polifenóis/administração & dosagem , Polifenóis/farmacologia , Água/análise
20.
AAPS PharmSciTech ; 21(5): 151, 2020 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-32440910

RESUMO

Triple-negative breast (TNBC) cancer that is upregulated with epidermal growth factor receptor (EGFR), and devoid of both the hormonal receptors and epidermal growth factor receptor 2 (HER 2), has led to a concept of treating TNBC with EGFR-targeted therapeutics. The combination of paclitaxel (PTX) and piperine (PIP) may improve the bioavailability of paclitaxel for cancer therapy. TPGS (vit E-PEG 1000-succinate)-coated liposomes were prepared with PTX alone or in combination with PIP, and either with (targeted) or without (non-targeted) cetuximab (CTX) conjugation. The Bradford assay indicated that 75% of CTX has been conjugated on the liposomes. The size and percent encapsulation of PTX&PIP co-loaded liposomes were found to be in the range of 204 to 218 nm and 31-73%, respectively. The drug release rate was found to be higher at pH 5.5 in comparison with release at pH 6.4 and pH 7.4. Cellular uptake and toxicity studies on MDA-MB-231 cells showed that PTX&PIP co-loaded targeted liposomes have demonstrated superior uptake and cytotoxicity than their non-targeted counterparts. The IC50 values of both of the liposomal formulations were found to be significantly higher than PTX control. Indeed, combining PIP with PTX control has improved the cytotoxicity of PTX control, which proved the synergistic anticancer effect of PIP. Lyophilized liposomes showed an excellent stability profile with the size range between 189 and 210 nm. Plasma stability study revealed a slight increase in the particle size due to the adsorption of plasma proteins on the surface of liposomes. The long-term stability study also indicated that liposomes were stable at 4°C.


Assuntos
Alcaloides/uso terapêutico , Antineoplásicos Fitogênicos/uso terapêutico , Benzodioxóis/uso terapêutico , Paclitaxel/uso terapêutico , Piperidinas/uso terapêutico , Alcamidas Poli-Insaturadas/uso terapêutico , Neoplasias de Mama Triplo Negativas/tratamento farmacológico , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/metabolismo , Antineoplásicos Fitogênicos/farmacologia , Protocolos de Quimioterapia Combinada Antineoplásica , Benzodioxóis/farmacologia , Linhagem Celular Tumoral , Composição de Medicamentos , Estabilidade de Medicamentos , Sinergismo Farmacológico , Receptores ErbB/efeitos dos fármacos , Feminino , Liofilização , Humanos , Lipossomos , Paclitaxel/metabolismo , Paclitaxel/farmacologia , Piperidinas/farmacologia , Alcamidas Poli-Insaturadas/farmacologia , Receptor ErbB-2
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