Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 104.647
Filtrar
1.
J Assoc Physicians India ; 68(2): 27-30, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32009358

RESUMO

Aims and Objectives: To evaluate the adequacy of glycaemic, lipid and blood pressure (BP) goals of diabetic patients, To evaluate the differences in goal attainment rates between various parameters like age, sex, body mass index, complications, medications and compliance. Materials and Methods: A total of 250 diabetic patients coming to HIMS, Hassan were studied and evaluated for BP, fasting blood sugar (FBS) , post prandial blood sugar (PPBS), glycosylated haemoglobin (HbA1c), low density lipoprotein (LDL), high density lipoprotein (HDL) and triglycerides (TG). Patients were evaluated and reviewed past medical records for complications. All diabetics on treatment were included in the study; newly diagnosed, type-1 diabetes, seriously ill, pregnant and those who don't want to be the part of study were excluded. Study Design: Case series study. Results: Out of 250 patients, 55.6% were males, 44.4% were females. BP, FBS and PPBS were tested in all patients. HbA1C was tested in 72%. Lipid parameters like LDL, HDL, and TG were tested in 42%, 37.6% and 52.4% respectively. FBS, PPBS and HBA1C target goals were achieved in 43.89%, 63.89% and 11.67% respectively in patients with complications and 77%, 88.50% and 68.5% respectively in those without complications. LDL, HDL and TG target goals were achieved in 37.78%, 47.22% and 41.11% respectively in patients with complications and 67.1%, 67.20% and 71.4% respectively in those without complications. BP goal was achieved in 53.89% and 78.55% in patients with and without complications respectively.


Assuntos
Glicemia , Diabetes Mellitus Tipo 2 , Pressão Sanguínea , Feminino , Hemoglobina A Glicada , Metas , Humanos , Lipídeos , Masculino
2.
Science ; 367(6475): 246-247, 2020 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-31949066
3.
Expert Opin Pharmacother ; 21(3): 353-363, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31893957

RESUMO

Introduction: Scientific evidence on subjects treated with statin or other lipid-lowering treatments has established that treatments aiming to lower low-density lipoprotein cholesterol (LDL-C) can reduce atherosclerosis. PCSK9 inhibitors (PCSK9-i), thanks to their efficacy in reducing LDL-C constitute a further step in the treatment of dyslipidemia and cardiovascular (CV) diseases.Areas covered: The purpose of this narrative review is to summarize the current knowledge of PCSK9-i, with particular regard to pharmacodynamic, pharmacokinetic, and clinical data on evolocumab and alirocumab.Expert opinion: PCSK9-I are effective in reducing atherosclerotic events through their significant LDL-C-lowering action similarly to statins. Furthermore, these drugs can be considered safe and well-tolerated. However, some controversies remain with regard to their efficacy in reducing mortality and the paucity of data on both pleiotropic effects and long-term safety of these drugs. However, future studies will focus on understanding the effects of very low cholesterol levels on health. At present, we know that the genetic model of PCSK9 deficiency is characterized by very low LDL-C levels without particular health problems. Yet, we do not know the effect of prolonged PCSK9 inhibition induced by antibody action during the lifetime of normal subjects.


Assuntos
Hipercolesterolemia/tratamento farmacológico , Pró-Proteína Convertase 9/antagonistas & inibidores , Anticorpos Monoclonais Humanizados/administração & dosagem , Anticolesterolemiantes/uso terapêutico , Doenças Cardiovasculares/tratamento farmacológico , LDL-Colesterol/sangue , Dislipidemias/tratamento farmacológico , Humanos , Inibidores de Hidroximetilglutaril-CoA Redutases/uso terapêutico , Lipídeos/sangue
4.
Bioresour Technol ; 300: 122561, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31911313

RESUMO

In this study, co-fermentation of primary sludge (PS) or waste activated sludge (WAS) with lipids was explored to improve volatile fatty acid production. PS and WAS were used as base substrate to facilitate lipid fermentation at 20 °C under semi-aerobic conditions. Mono-fermentation tests showed higher VFA yields for PS (32-89 mgCOD gVS-1) than for WAS (20-41 mgCOD gVS-1) where propionate production was favoured. The principal component analysis showed that the base substrate had a notable influence on co-fermentation yields and profile. Co-fermentation with WAS resulted in a greater extent of oleic acid degradation (up to 4.7%) and evidence of chain elongation producing valerate. The occurrence of chain elongation suggests that co-fermentation can be engineered to favour medium-chain fatty acids without the addition of external commodity chemicals. BMP tests showed that neither mono-fermentation nor co-fermentation had an impact on downstream anaerobic digestion.


Assuntos
Lipídeos , Esgotos , Anaerobiose , Reatores Biológicos , Ácidos Graxos Voláteis , Fermentação , Propionatos
7.
Bioresour Technol ; 301: 122781, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31954963

RESUMO

To establish the models of microbial lipid production from cellulosic ethanol wastewater by R. glutinis, the biomass, lipid yield, and COD removal rate were investigated under different conditions. Subsequently, the genetic algorithm based on SVM was adopted to optimize parameters for obtaining the maximum biomass. The results demonstrated that the initial COD and glucose content had a significant effect on lipids synthesis. Most of the organic matter in the wastewater was consumed with the production of lipid. Compared with BP-ANN, SVM had better fitting and generalization ability for small amount of experimental data. By genetic algorithm optimization based on SVM, the maximum biomass and lipid yield could reach 11.87 g/L and 2.18 g/L, respectively. The results suggest that the SVM model could be used as an effective tool to optimize fermentation conditions.


Assuntos
Rhodotorula , Águas Residuárias , Biomassa , Etanol , Fermentação , Lipídeos , Máquina de Vetores de Suporte
8.
Bioresour Technol ; 301: 122762, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31972402

RESUMO

To assess the potential of Chlorella sp. GN1 for producing biodiesel raw materials in flat plate photobioreactors (FPPs) outdoors, we optimized the nitrogen sources and concentrations for the growth of the algae. The effects of different light paths of FPPs on the growth, lipid accumulation, and fatty acids of Chlorella sp. GN1 were also studied. As the light path of the FPPs was reduced, the alga could accumulate lipids rapidly, achieving high lipid content and lipid productivity outdoors. The highest lipid content obtained was 53.5%, when the light path was 5 cm. In addition, the lipid productivity was 66.7 mg L-1 day-1. The main fatty acids were C16/C18, accounting more than 90% of the total fatty acids. Results showed that Chlorella sp. GN1 had the ability to accumulate large quantities of lipids in FPPs outdoors and was a promising microalgal species for biofuel production.


Assuntos
Chlorella , Microalgas , Biocombustíveis , Biomassa , Ácidos Graxos , Luz , Lipídeos , Nitrogênio , Fotobiorreatores
9.
Medicine (Baltimore) ; 99(1): e18553, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31895796

RESUMO

BACKGROUND: Type 2 Diabetes Mellitus (T2DM) is a chronic disease that is increasing the number of cases worldwide. The treatments currently used have not worked as expected. Alternative and complementary medicines were inserted in health services, especially in primary care, as an attempt to minimize risks and help control diseases such as diabetes. Among the herbal medicines used stands out cinnamon, which can serve as an adjuvant in the control of diabetes. OBJECTIVE: To analyze the effectiveness of 3 grams of cinnamon (Cinnamomum verum) per day for 90 days in reducing glycemic and lipid levels in adults with T2DM compared with placebo METHODS:: A randomized, double-blind, placebo-controlled, phase II trial, which will be conducted at basic health units in the city of Parnaíba, state of Piauí, Brazil. In total, 130 people diagnosed with T2DM, followed at health units, with hemoglobin A1c > 6.5%, and using oral antidiabetic medicines, are expected to participate in the study. The intervention will last for 3 months, and each participant will receive a total of 3 bottles containing 120 capsules in each bottle of cinnamon or placebo. Each person should take 4 capsules daily, for 90 days. The patients will be distributed into the 2 groups by performing block randomization (n = 6) at a ratio of 1:1 according to a code generated by a software. Assessments of socioeconomic, clinical, lifestyle, anthropometric, and laboratory variables will be performed in 2 separate visits. DISCUSSION: This study will be the first to investigate cinnamon to reduce glycemic, lipid, and anthropometric levels in Brazil. In case of favorable results, this therapy may be used as an alternative or additional medicine in cases where only oral antidiabetic agents are used and can promote the use of the product to minimize future complications of patients with diabetes and people who do not have the disease. TRIAL REGISTRATION: RBR-2KKB6D, registered on December 11th, 2018.


Assuntos
Glicemia/efeitos dos fármacos , Cinnamomum zeylanicum , Diabetes Mellitus Tipo 2/tratamento farmacológico , Hipoglicemiantes/administração & dosagem , Lipídeos/sangue , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Ensaios Clínicos Fase II como Assunto , Diabetes Mellitus Tipo 2/sangue , Método Duplo-Cego , Feminino , Hemoglobina A Glicada/efeitos dos fármacos , Humanos , Masculino , Pessoa de Meia-Idade , Ensaios Clínicos Controlados Aleatórios como Assunto , Adulto Jovem
10.
Medicine (Baltimore) ; 99(2): e18550, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914031

RESUMO

BACKGROUND: Adjuvant endocrine therapy is a vital portion of postoperative comprehensive treatment for breast cancer patients. In recent years, studies have shown that endocrine therapy has a certain impact on the serum lipids of breast cancer patients, and the changes of lipid profiles may bring a series of problems. However, very few studies focus on this issue to date. The results of these studies are inconsistent, and the influence of different adjuvant endocrine modalities on lipid profiles still remains controversial. In order to better explore this issue, we conduct this network meta-analysis. METHOD: The protocol followed preferred reporting items for systematic reviews and meta-analyses protocols. Three main databases (PubMed, Embase, and the Cochrane Library) will be searched systematically for eligible randomized controlled trials without language restriction. In addition, a manual search of the references of relevant published studies will also be considered. Two reviewers will conduct studies selection, data extraction, and risk of bias assessment independently. The primary outcome is the variation of biochemical parameters - the serum lipid profiles (cholesterol, triglyceride, high-density lipoprotein, low low-density lipoprotein). RESULTS: The results will provide useful information about the side effects of different adjuvant endocrine drugs on lipid profiles in postoperative breast cancer patients (estrogen receptor-positive and/or progesterone receptor-positive). CONCLUSION: The findings of this study will be published in a peer-reviewed journal. PROSPERO REGISTRATION NUMBER: CRD42019129850.


Assuntos
Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Neoplasias da Mama/tratamento farmacológico , Lipídeos/sangue , Anastrozol/farmacologia , Anastrozol/uso terapêutico , Androstadienos/farmacologia , Androstadienos/uso terapêutico , Inibidores da Aromatase/farmacologia , Inibidores da Aromatase/uso terapêutico , Quimioterapia Adjuvante , Humanos , Letrozol/farmacologia , Letrozol/uso terapêutico , Meta-Análise em Rede , Projetos de Pesquisa , Tamoxifeno/farmacologia , Tamoxifeno/uso terapêutico , Toremifeno/farmacologia , Toremifeno/uso terapêutico
11.
Medicine (Baltimore) ; 99(2): e18753, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914097

RESUMO

The aims of this study were to evaluate the prevalence of metabolic syndrome (MetS) and explore the association between sleep duration and MetS. This study enrolled 8 272 adults aged 18 years and older from 6 urban and 8 rural areas during 2013 to 2014in Henan China. Participants were interviewed about demographic characteristics, lifestyle factors and medical history, and physical measurements were performed. The relationships between sleep duration and MetS were evaluated and plotted by Restricted Cubic Spline Regression. The mean age was 51.5 years (SD 14.2) and 4 916 (59.4%) were female. The crude prevalence of MetS was 30.3% and the age-standardized rate was 23.6%. Men were more likely to have MetS than women (P = .01). MetS was positively associated with age, education, smoking, drinking, BMI and sleep duration, and seemed irrelevant to occupation and sedentary behavior. In terms of individual component of MetS, high blood pressure was the most prevalent component for both men and women, while the lowest prevalent was high triglycerides in men and for women was low high-density lipoprotein cholesterol (HDL-C). There was a U-shaped relationship between sleep duration and MetS and its components. Sleep duration <6 hours or >9 hours were associated with higher risk of MetS (OR from 1.10 to 2.15). The MetS was prevalent, and more than half of total adult population was suffering from high blood pressure. Sleep duration may be a determinant of metabolic health. Both short (<6 hours) and long sleep duration (>9 hours) was linked to an increased risk of MetS.


Assuntos
Síndrome Metabólica/epidemiologia , Sono/fisiologia , Adulto , Fatores Etários , Idoso , Consumo de Bebidas Alcoólicas/epidemiologia , Glicemia , Índice de Massa Corporal , Feminino , Humanos , Hipertensão/epidemiologia , Estilo de Vida , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade , Fatores Sexuais , Fumar/epidemiologia , Fatores Socioeconômicos , Fatores de Tempo
12.
Phys Chem Chem Phys ; 22(3): 1738-1746, 2020 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-31898698

RESUMO

Cationic liposomes, a type of non-viral vectors, often play the important biological function of delivering nucleic acids during cell transfection. Variations in the molecular architecture of di-alkyl dihydroxy ethyl ammonium chloride-based cationic lipids involving hydrophobic tails have been found to influence their biological function in terms of cell transfection efficiency. For example, liposomes based on a cationic lipid (Lip1814) with asymmetry in the hydrophobic chains were found to display higher transfection efficacy in cultured mammalian cell lines than those comprising of symmetric Lip1818 or asymmetric Lip1810. The effect of variations in the molecular architecture of the cationic lipids on the biological activity of liposomes has been explored here via the photophysical studies of 8-anilino-1-naphthalenesulphonate (ANS) and Nile Red (NR) in three cationic liposomes, namely Lip1810, Lip1814 and Lip1818. Time-resolved fluorescence of ANS revealed reduced hydration at the lipid-water interface and enhanced relaxation dynamics of surface water (lipid headgroup bound water molecules) in Lip1810- and Lip1814-based liposomes in the presence of cholesterol. As the probe ANS failed to be incorporated into the lipid-water interface of Lip1818 due to the significantly high rigidity of these liposomes, no information concerning the extent of hydration of the lipid-water interface or the interfacial water dynamics could be obtained. Time-resolved polarization-gated anisotropy measurements of NR in the presence of cholesterol revealed the rigidity of the cationic liposomes to be increasing in the order of Lip1810 < Lip1814 < Lip1818. In the presence of cholesterol, moderately higher rigidity, reduced membrane hydration and enhanced relaxation dynamics of the interfacial water molecules gave rise to the superior cell transfection efficacy of Lip1814-based cationic liposomes than those of the highly flexible Lip1810 or the highly rigid Lip1818.


Assuntos
Lipídeos/química , Linhagem Celular , Interações Hidrofóbicas e Hidrofílicas , Modelos Moleculares , Conformação Molecular , Transfecção
13.
Zhongguo Dang Dai Er Ke Za Zhi ; 22(1): 65-70, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-31948527

RESUMO

OBJECTIVE: To study the effect of epigallocatechin-3-gallate (EGCG) on liver lipid metabolism in rats with intrauterine growth restriction (IUGR) and related mechanism. METHODS: A rat model of IUGR was established by food restriction during entire pregnancy, and then the rats were randomly divided into an IUGR group and an EGCG group (n=8 each). The rats in the EGCG group were fed with water containing EGCG from after weaning to 10 weeks. Eight pup rats born from the pregnant maternal rats without food restriction were used as the control group. At the age of 13 weeks, body weight was measured. Blood and liver tissue samples were collected to measure fasting total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), fasting plasma glucose (FPG), fasting insulin (FINS), and liver lipids. Homeostasis model assessment of insulin resistance (HOMA-IR) and adipose insulin resistance (adipo-IR) were calculated. Pathological sections of the liver were observed and quantitative real-time PCR was used to measure the mRNA expression of related genes in the liver. RESULTS: At the age of 13 weeks, there was no significant difference in body weight between groups (P=0.067). There were significant differences between groups in FPG, FFA, FINS, HOMA-IR, and adipo-IR (P<0.05). There were no significant differences in the serum levels of TC and TG between groups (P>0.05), while the IUGR group had significantly higher levels of TC and TG in the liver than the EGCG group (P<0.05). Oil red staining showed that the IUGR group had a significant increase in hepatic lipid accumulation, while the EGCG group had certain improvement after EGCG treatment. PCR results suggested that compared with the control group, the IUGR group had significant reductions in the mRNA expression of Ampk and Adipor1 and a significant increase in the mRNA expression of Srebf1 (P<0.05), while EGCG increased the mRNA expression of Ampk and reduced the mRNA expression of Srebf1, with no significant differences in the two indices between the EGCG and control groups (P>0.05). CONCLUSIONS: Early EGCG intervention can down-regulate the de novo synthesis of fatty acids through the Ampk/Srebf1 signaling pathway and reduce hepatic lipid accumulation in IUGR rats by improving insulin resistance of hepatocytes.


Assuntos
Resistência à Insulina , Metabolismo dos Lipídeos , Animais , Catequina/análogos & derivados , Feminino , Retardo do Crescimento Fetal , Lipídeos , Fígado , Gravidez , Ratos
14.
Adv Exp Med Biol ; 1232: 183-190, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31893409

RESUMO

Oxygen delivery to tissue mitochondria relies on simple diffusion in the target cells and tissues. As such, intracellular availability of O2 in tissue depends on its solubility and diffusivity in complex and heterogeneous macromolecular environments. The path of oxygen diffusion is key to its rate of transfer, especially where pathways of differing favorability are present. Most commonly, aqueous media, such as interstitial fluid and cytoplasm, are assumed to provide the dominant diffusion path. Here, the 'hydrophobic channeling' hypothesis is revisited, and several lines of evidence pointing toward lipid-accelerated oxygen diffusion pathways are discussed. The implications of hydrophobic channeling are considered in light of extended membrane networks in cells and tissues.


Assuntos
Células , Difusão , Metabolismo dos Lipídeos , Oxigênio , Células/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Lipídeos/química , Mitocôndrias/metabolismo , Oxigênio/metabolismo
15.
Bioresour Technol ; 297: 122404, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31757613

RESUMO

In this study, cost simulations were made based on 20 million L blended biodiesel B-10 production per year using INRS and conventional process. In case of INRS process, microbial lipid was produced by T. oleaginosus using washed municipal secondary sludge fortified with crude glycerol while lipid was extracted from wet biomass using biodegradable surfactant and petroleum-diesel (PD). The conventional process uses commercial substrates for lipid production and organic solvents for lipid extraction from dry biomass. The unit B-10 production cost of INRS process was estimated to be $ 0.72/L for an annual capacity of 20 million L, which is 9.5 times more economical than conventional biodiesel production process. For INRS process, the unit B-10 biodiesel production cost was sensitive to plant capacity and lipid productivity during the fermentation. INRS process exhibited positive net energy gain and positive GHG capture, which proves to be energetically and environmentally viable.


Assuntos
Biocombustíveis , Glicerol , Biomassa , Lipídeos , Esgotos
16.
Bioresour Technol ; 297: 122509, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31812914

RESUMO

Dunaliella salina is well-known for its high content in carotenoids and glycerol. Nevertheless, Dunaliella salina has also a high content in lipids, including polar lipids, which are suitable for nutraceutical/cosmeceutical applications. This work proposes a sustainable process to maximise the potential of Dunaliella salina for the production of distinct fractions of carotenoids, glycerol, polar lipids and proteins, which may contribute to improve the revenues of the microalgae industry. In this work, extraction with non-hazardous solvents and organic solvent nanofiltration are integrated, in order to obtain added-value products and glycerol. Also, aiming to separate carotenoids from glycerides, a saponification process is proposed. High overall recoveries were obtained for carotenoids (85%), glycerol (86%), polar lipids (94%) and proteins (95%). In order to evaluate the profitability of the proposed biorefinery, an economic assessment was accomplished. Both CAPEX and OPEX (Capital and Operating expenditure) were calculated, likewise the Return of Investment (ROI).


Assuntos
Carotenoides , Microalgas , Glicerídeos , Glicerol , Lipídeos
17.
Bioresour Technol ; 297: 122439, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31810740

RESUMO

In the present study, a combination of phytohormones (indole acetic acid and kinetin) was augmented in nitrogen-limited medium to achieve higher biomass and lipid yield in Graesiella emersonii NC-M1 and Chlorophyta sp. NC-M5. This condition was recorded with a 2.3- and 2.5-fold increase in biomass and lipid yield for Graesiella emersonii NC-M1 compared to the nitrogen-limited condition. Also, this condition showed a 1.6- and 1.08-fold increase in lipid yield and neutral lipid compared to the standard condition. Phytohormones addition also reduced oxidative damage caused by nitrogen-limitation and enhanced monounsaturated fatty acid content. Further, a 5.2- and 3.17-fold enhance in expression level of GPAT and DGAT genes were noticed under nitrogen-limited medium supplemented with phytohormones compared to control.


Assuntos
Microalgas , Biocombustíveis , Biomassa , Diacilglicerol O-Aciltransferase , Ácidos Graxos , Glicerol , Ácidos Indolacéticos , Cinetina , Lipídeos , Nitrogênio , Fosfatos
18.
Biomed Chromatogr ; 34(1): e4712, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31633799

RESUMO

Coffee and tea are the most widely consumed beverages worldwide. However, the consumer may be unaware of the exact amount of methyl xanthine (MX, i.e. caffeine [C], theobromine [TB] and theophylline [TH]) consumed, as most of the products do not list the proper amounts. This may lead to serious risks including cardiovascular, kidney and stimulant effects. The aim of the study was to determine the MX amount in ready-to-use beverages (coffee and tea) collected from various outlets in the city of Al-Khobar, Saudi Arabia. Forty different samples of espresso, black coffee and red tea were collected. A fast, reliable and efficient UHPLC-DAD method was developed and validated for MX determination. Total lipids were extracted and fractionated in order to determine glycolipids, phospholipids and neutral lipids. The r2 value for the method was 0.980-0.988 in a linearity range of 0.5-200 ppm. The range for MX (C [0.02-2.39 mg/ml], TB [0.00-0.10 mg/ml] and TH [0.00-0.004 mg/ml]) and total lipids was 1-5 g. The amount of glycolipids (3.1 g) was higher among the lipid fractions followed by phospholipids (1.8 g) and neutral lipids (0.25 g). In general, espresso beverages (20-30 ml) contained high amounts of MX whereas black coffee beverages contained high amount of lipids. Most of the beverages expressed C, TB, TH, lipids or their fractions; however, the product with high amounts of MX and lipids at the same time was espresso (brands Chemistry and Wogard). Although the MX and lipid levels in these beverages well below the allowed limits, care must still be taken, especially when using the beverages with high serving volumes (200-250 ml) or coffee prepared via the filter method i.e. black coffee, using a high temperature for a longer time.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Café/química , Chá/química , Xantinas , Culinária , Temperatura Alta , Lipídeos/análise , Reprodutibilidade dos Testes , Arábia Saudita , Xantinas/análise , Xantinas/química , Xantinas/isolamento & purificação
19.
J Sci Food Agric ; 100(3): 1022-1029, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31646643

RESUMO

BACKGROUND: Pork is used as raw material to produce Cantonese sausage, and 0.5 or 1 g kg-1 of d-sodium erythorbate is added to the pork meat. In this study the myoglobin oxidation rate, relative metmyoglobin content, heme iron content, redness, pH, free radical content and thiobarbituric acid reactive substance (TBARS) value were measured at different processing times and different content of d-sodium erythorbate. RESULTS: It was found that d-sodium erythorbate significantly reduced the free radical content and myoglobin and lipid oxidation rates and increased heme iron levels. When d-sodium erythorbate was added to the sausage, the absorption peak of myoglobin porphyrin shifted left, migrating from 414 to 405 nm. At 72 h, with an increase in the d-sodium erythorbate content, a significant negative correlation was identified between heme iron and the degree of redness (P < 0.01). CONCLUSION: During sausage processing, there are strong correlations among TBARS values, free radical content, metmyoglobin levels, heme iron levels, a* and pH at the same d-sodium erythorbate level. At the same processing time, adding d-sodium erythorbate can slow the rate of myoglobin and lipid oxidation and prevent the discoloration of sausage. © 2019 Society of Chemical Industry.


Assuntos
Ácido Ascórbico/análise , Aditivos Alimentares/análise , Lipídeos/química , Produtos da Carne/análise , Mioglobina/química , Animais , Cor , Manipulação de Alimentos , Metamioglobina/química , Oxirredução , Suínos
20.
J Sci Food Agric ; 100(3): 1246-1255, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31696520

RESUMO

BACKGROUND: Chinese mutton ham is a dry-cured meat product with a long ripening time. The aim of this study was to identify and characterize antioxidant peptides from Chinese mutton ham. RESULTS: Mutton ham peptides (MHPs) were purified by gel filtration, anion exchange and reversed-phase high-performance liquid chromatography steps. The 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging capacity was used to guide the purification of MHPs. Three antioxidant peptides were identified by liquid chromatography with tandem mass spectrometry (LC-MS/MS) as Met-Trp-Thr-Asp (MWTD), Ala-Pro-Tyr-Met-Met (APYMM) and Phe-Trp-Ile-Ile-Glu (FWIIE), with molecular weights 551.61, 611.76, and 706.84 Da, respectively. Among them, APYMM exhibited the highest ABTS radical scavenging activity. The three peptides had the ability to inhibit lipid oxidation and Fenton's reagent-induced protein oxidation and DNA damage. After simulated gastrointestinal digestion, FWIIE and APYMM showed increased antioxidant activity, while MWTD showed decreased activity. CONCLUSION: Three novel peptides isolated from Chinese mutton ham had strong biological activity. Chinese mutton ham is potentially a functional food and an excellent source of natural antioxidants. © 2019 Society of Chemical Industry.


Assuntos
Antioxidantes/química , Produtos da Carne/análise , Peptídeos/química , Sequência de Aminoácidos , Animais , China , Cromatografia Líquida , Lipídeos/química , Oxirredução , Mapeamento de Peptídeos , Suínos , Espectrometria de Massas em Tandem
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA