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1.
Bioresour Technol ; 304: 123061, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32127245

RESUMO

Selection of indigenous and potential algal strain with high lipid content is paramount challenge in the avenues of microalgal biodiesel production. Particularly, hyper lipid producing algae with maximal triacyglycerols (TAGs) content and preferable fatty acid composition is of interest for sustainable biodiesel. Hence, the present study on comparative assessment of Chlorella vulgaris, Scenedesmus sp. and Synechococcus sp. was done in terms of cell density, lipid, TAGs and fatty acid. Higher biomass yield was obtained in Chlorella vulgaris (0.54 gL-1) on 13th day while maximal lipid content of 36% was observed in Scenedesmus sp. followed by Chlorella vulgaris (33%). Lipidomic analysis revealed higher non-polar lipids inChlorella vulgaris (57%) and Scenedesmus sp. (54%), whereas in Synechococcus sp. 69% polar lipids were present. In fatty acid profile, C24:0 (22.11%) was predominant in Chlorella vulgaris, while C20:0 (31.72%) and C18:2 (22.26%) was prevalent in Scenedesmus sp. and Synechococcus sp. respectively.


Assuntos
Bioprospecção , Chlorella vulgaris , Microalgas , Biocombustíveis , Biomassa , Contagem de Células , Ácidos Graxos , Lipidômica , Seleção de Pacientes
2.
PLoS One ; 15(3): e0229868, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32163448

RESUMO

The purpose of this study was to examine the influence of medium-chain fatty acid-containing triglycerides (MCT), long-chain polyunsaturated fatty acid-containing triglycerides, and their combination on the plasma metabolome of cats (Felis catus), including circulating microbiome-derived postbiotics. After a 14-day lead-in on the control food, cats were randomized to one of four foods (control, with 6.9% MCT, with fish oil [FO; 0.14% eicosapentaenoate, 1.0% docosahexaenoate], or with FO+MCT; n = 16 per group) for 28 days. Analysis of plasma metabolites showed that the addition of FO and MCT led to synergistic effects not seen with either alone across a number of lipid classes, including fatty acids, acylcarnitines, and acylated amines including endocannabinoids. Notably, the FO+MCT group had an increase in ketone body production relative to baseline and beyond that seen with MCT alone. N-acyl taurines, the accumulation of which has been implicated in the onset of type 2 diabetes, were significantly decreased in the FO+MCT group. Significant decreases in the gut microbiome-derived postbiotic classes of indoles/indolic sulfates and phenols/phenolic sulfates were observed only the FO+MCT group. Overall, the combination of MCT and FO led to number of changes in plasma metabolites that were not observed with either oil alone, particularly in postbiotics.


Assuntos
Ração Animal , Ácidos Docosa-Hexaenoicos/administração & dosagem , Microbioma Gastrointestinal/efeitos dos fármacos , Metabolismo dos Lipídeos/efeitos dos fármacos , Triglicerídeos/administração & dosagem , Animais , Gatos , Feminino , Indóis/sangue , Indóis/metabolismo , Corpos Cetônicos/sangue , Corpos Cetônicos/metabolismo , Lipidômica , Lipídeos/sangue , Masculino , Fenóis/sangue , Fenóis/metabolismo
3.
Food Chem ; 318: 126504, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32146310

RESUMO

Tuna adulteration and mislabeling are serious problem worldwide and have caused economic loss and consumer rights violation. In this study, an electrometric knife (iKnife) coupling rapid evaporative ionization mass spectrometry (REIMS) and a multivariate recognition model were developed and employed for in situ and real-time authentication of four tuna species without sample preparation. The results showed that the lipidomic profiles were successfully acquired and the differences in fatty acids and phospholipids were statistically analyzed to be significant (p < 0.05). The model displayed the superb classification accuracy (>93%) and validation (R2(Y) = 0.992, Q2 = 0.986), and the main contributors of m/z 817.64, m/z 809.68, etc. were screened out to be used as potential biomarkers. Based on this technique, the identity of blind tuna samples could be unambiguously authenticated with the results displayed on a monitor screen directly. This study provided a front-line rapid detection method to prove the authenticity of tuna species.


Assuntos
Contaminação de Alimentos/análise , Lipidômica/métodos , Espectrometria de Massas/métodos , Animais , Ácidos Graxos/química , Fosfolipídeos/química , Atum
4.
Life Sci ; 245: 117352, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32006527

RESUMO

AIMS: The depot-specific differences in lipidome of visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) reflect heterogeneity of white adipose tissue (WAT), which plays a central role in its distinct response to outside stimuli. However, the detailed lipidome of depot-specific WAT is largely unknown, especially the minor constitutes including phospholipid and sphingolipid. MATERIALS AND METHODS: To investigate this field, we applied a high-coverage targeted lipidomics approach of VAT and SAT in male C57BL/6J mice to compare the basal level of their lipid profiles. Applying microarray and quantitative real-time polymerase chain reaction, we analyzed the transcriptome of twodepot-specific WAT and verified the differences in individual genes. KEY FINDINGS: In total, 342 lipid species from 19 lipid classes were identified. Our results showed the composition of TAG and FFA were different in length of chain and saturation. Interestingly, low abundance phospholipid, sphingolipid and cardiolipin were significantly higher in SAT. Lipid correlation network analysis vindicated that TAG and phospholipid formed distinct subnet and had more connections with other lipid species. Enriched ontology analysis of gene screened from LIPID MAPS and microarray suggested the differences were mainly involved in lipid metabolism, insulin resistance and inflammatory response. SIGNIFICANCE: Our comprehensive lipidomics and transcriptomics analyses revealed differences in lipid composition and lipid metabolism of two depot-specific WAT, which would offer new insights into the investigation of heterogeneity of visceral and subcutaneous white adipose tissue.


Assuntos
Tecido Adiposo Branco/metabolismo , Gordura Intra-Abdominal/metabolismo , Lipidômica , Gordura Subcutânea/metabolismo , Transcriptoma , Tecido Adiposo Branco/química , Animais , Cardiolipinas/análise , Cardiolipinas/metabolismo , Ceramidas/análise , Ceramidas/metabolismo , Ácidos Graxos/análise , Ácidos Graxos/metabolismo , Glicerídeos/análise , Glicerídeos/metabolismo , Gordura Intra-Abdominal/química , Metabolismo dos Lipídeos , Lipídeos/análise , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Fosfolipídeos/análise , Fosfolipídeos/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Gordura Subcutânea/química
5.
Ecotoxicol Environ Saf ; 192: 110254, 2020 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-32007746

RESUMO

The direct interactions of bacterial membranes and polycyclic aromatic hydrocarbons (PAHs) strongly influence the biological processes, such as metabolic activity and uptake of substrates due to changes in membrane lipids. However, the elucidation of adaptation mechanisms as well as membrane phospholipid alterations in the presence of phenanthrene (PHE) from α-proteobacteria has not been fully explored. This study was conducted to define the degradation efficiency of PHE by Sphingopyxis soli strain KIT-001 in a newly isolated from Jeonju river sediments and to characterize lipid profiles in the presence of PHE in comparison to cells grown on glucose using quantitative lipidomic analysis. This strain was able to respectively utilize 1-hydroxy-2-naphthoic acid and salicylic acid as sole carbon source and approximately 90% of PHE (50 mg/L) was rapidly degraded via naphthalene route within 1 day incubation. In the cells grown on PHE, strain KIT-001 appeared to dynamically change profiles of metabolite and lipid in comparison to cells grown on glucose. The levels of primary metabolites, phosphatidylethanolamines (PE), and phosphatidic acids (PA) were significantly decreased, whereas the levels of phosphatidylcholines (PC) and phosphatidylglycerols (PG) were significantly increased. The adaptation mechanism of Sphingopyxis sp. regarded mainly the accumulation of bilayer forming lipids and anionic lipids to adapt more quickly under restricted nutrition and toxicity condition. Hence, these findings are conceivable that strain KIT-001 has a good adaptive ability and biodegradation for PHE through the alteration of phospholipids, and will be helpful for applications for effective bioremediation of PAHs-contaminated sites.


Assuntos
Fenantrenos/metabolismo , Fosfolipídeos/metabolismo , Sphingomonadaceae/metabolismo , Biodegradação Ambiental , Sedimentos Geológicos/microbiologia , Lipidômica , Metabolômica , Naftalenos/metabolismo , Naftóis/metabolismo , Fosfolipídeos/química , Ácido Salicílico/metabolismo , Sphingomonadaceae/isolamento & purificação
6.
Ann Rheum Dis ; 79(4): 499-506, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32079570

RESUMO

OBJECTIVES: The differential diagnosis of seronegative rheumatoid arthritis (negRA) and psoriasis arthritis (PsA) is often difficult due to the similarity of symptoms and the unavailability of reliable clinical markers. Since chronic inflammation induces major changes in the serum metabolome and lipidome, we tested whether differences in serum metabolites and lipids could aid in improving the differential diagnosis of these diseases. METHODS: Sera from negRA and PsA patients with established diagnosis were collected to build a biomarker-discovery cohort and a blinded validation cohort. Samples were analysed by proton nuclear magnetic resonance. Metabolite concentrations were calculated from the spectra and used to select the variables to build a multivariate diagnostic model. RESULTS: Univariate analysis demonstrated differences in serological concentrations of amino acids: alanine, threonine, leucine, phenylalanine and valine; organic compounds: acetate, creatine, lactate and choline; and lipid ratios L3/L1, L5/L1 and L6/L1, but yielded area under the curve (AUC) values lower than 70%, indicating poor specificity and sensitivity. A multivariate diagnostic model that included age, gender, the concentrations of alanine, succinate and creatine phosphate and the lipid ratios L2/L1, L5/L1 and L6/L1 improved the sensitivity and specificity of the diagnosis with an AUC of 84.5%. Using this biomarker model, 71% of patients from a blinded validation cohort were correctly classified. CONCLUSIONS: PsA and negRA have distinct serum metabolomic and lipidomic signatures that can be used as biomarkers to discriminate between them. After validation in larger multiethnic cohorts this diagnostic model may become a valuable tool for a definite diagnosis of negRA or PsA patients.


Assuntos
Artrite Psoriásica/sangue , Artrite Reumatoide/sangue , Acetatos/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Alanina/sangue , Aminoácidos/sangue , Artrite Psoriásica/diagnóstico , Artrite Reumatoide/diagnóstico , Artrite Reumatoide/imunologia , Colina/sangue , Creatina/sangue , Diagnóstico Diferencial , Feminino , Humanos , Ácido Láctico/sangue , Lipidômica , Lipídeos/sangue , Masculino , Metaboloma , Metabolômica , Pessoa de Meia-Idade , Fosfocreatina/sangue , Espectroscopia de Prótons por Ressonância Magnética , Ácido Succínico/sangue
7.
PLoS One ; 15(2): e0223031, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32084137

RESUMO

Blue mussels (Mytilus edulis L. 1758) are important components of coastal ecosystems and in the economy of rural and coastal areas. The understanding of their physiological processes at key life stages is important both within food production systems and in the management of wild populations. Lipids are crucial molecules for bivalve growth, but their diversity and roles have not been fully characterised. In this study, traditional lipid profiling techniques, such as fatty acid (FA) and lipid class analysis, are combined to untargeted lipidomics to elucidate the lipid metabolism in newly settled spat fed on a range of diets. The evaluated diets included single strains treatments (Cylindrotheca fusiformis CCAP 1017/2 -CYL, Isochrysis galbana CCAP 927/1- ISO, Monodopsis subterranean CCAP 848/1 -MONO, Nannochloropsis oceanica CCAP 849/10- NANNO) and a commercial algae paste (SP). Spat growth was influenced by the diets, which, according to their efficacy were ranked as follows: ISO>NANNO/CYL>SP>MONO. A higher triacylglycerols (TG) content, ranging from 4.23±0.82 µg mgashfree Dry weight (DW)-1 at the beginning of the trial (T0) to 51±15.3 µg mgashfreeDW-1 in ISO, characterised significant growth in the spat, whereas, a reduction of TG (0.3±0.08 µg mgashfreeDW-1 in MONO), mono unsaturated FA-MUFA (from 8.52±1.02 µg mgFAashfreeDW-1 at T0 to 2.81±1.02 µg mgFAashfreeDW-1 in MONO) and polyunsaturated FA-PUFA (from 17.57±2.24 µg mgFAashfreeDW-1 at T0 to 6.19±2.49 µg mgFAashfreeDW-1 in MONO) content characterised poor performing groups. Untargeted lipidomics evidenced how the availability of dietary essential PUFA did not influence only neutral lipids but also the membrane lipids, with changes in lipid molecular species in relation to the essential PUFA provided via the diet. Such changes have the potential to affect spat production cycle and their ability to respond to the surrounding environment. This study evidenced the advantages of coupling different lipid analysis techniques, as each technique disclosed relevant information on nutritional requirements of M. edulis juveniles, expanding the existing knowledge on the physiology of this important species.


Assuntos
Ecologia/economia , Lipidômica/métodos , Mytilus edulis/química , Necessidades Nutricionais , Animais , Dieta/métodos , Ecossistema , Ácidos Graxos Insaturados/análise , Metabolismo dos Lipídeos
8.
Aquat Toxicol ; 221: 105421, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32036233

RESUMO

Lipids play important roles in growth, reproduction, locomotion, and migration of fish. Municipal effluents, which are complex mixtures of biological and chemical compounds including flame retardants, have been shown to alter lipid metabletabolism in environmentally and experimentally exposed fish. Down-regulation of several genes coding for fatty acid metabolism enzymes has previously been reported in male northern pike (Esox lucius) collected in the St. Lawrence River (QC, Canada) downstream of a major primary wastewater treatment plant (WWTP) point of discharge. The main objective of this study was to investigate the effects of exposure to the Montreal's WWTP effluent on the lipidomic profile (i.e., fatty acids, acylcarnitines, and phospholipids) as well as the transcription of genes related to lipid metabolism in the liver of northern pike collected upstream and downstream of this WWTP effluent. Halogenated flame retardant concentrations were also determined in pike liver and used as markers of exposure to this effluent. Greater concentrations of saturated and monounsaturated lysophosphatidylcholines (LPCs) and lower concentrations of polyunsaturated LPCs were determined in the liver of pike collected downstream of the WWTP compared to those collected upstream. Lower mRNA levels of peroxisome proliferator-activated receptor alpha (pparα), a major regulator of lipid metabolism, were also measured in pike exposed to Montreal's WWTP effluent. In addition, the relative contributions (%) of LPC 18:2 and LPC14:0, compounds used as markers of obesity and inflammation, were significantly correlated with halogenated flame retardant concentrations and fish girth. Results of the present study suggest that chronic environmental exposure to a primary WWTP effluent can modulate the transcription of genes related to lipid metabolism, and hence affect the hepatic phospholipid composition of pike from the St. Lawrence River.


Assuntos
Exposição Ambiental/efeitos adversos , Esocidae/metabolismo , Retardadores de Chama/toxicidade , Metabolismo dos Lipídeos/efeitos dos fármacos , Águas Residuárias/química , Poluentes Químicos da Água/toxicidade , Animais , Canadá , Exposição Ambiental/análise , Esocidae/genética , Metabolismo dos Lipídeos/genética , Lipidômica , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Rios/química , Transcrição Genética/efeitos dos fármacos
9.
Sci Total Environ ; 713: 136565, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-31954244

RESUMO

The stereoselective effects of chiral ibuprofen (IBU) were studied using lipidomics by exposing adult zebrafish (Danio rerio) to an environmental concentration of 5 µg/L for 28 days. After treatment with rac-/R-(-)-/S-(+)-IBU, the brain tissue of the zebrafish was harvested to analyze for lipid metabolites by using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. Results showed that the six classes of lipids, namely, glycerophospholipids, sterol lipids, prenol lipids, fatty acyls, glycerolipids, and sphingolipids, including 46 biomarkers, were affected after exposure. The different influences on metabolites were observed in the rac-/R-(-)-/S-(+)-IBU-treated samples. The rac-IBU treatment remarkably affected nine lipids. The R-(-)-IBU and S-(+)-IBU treatments had remarkably effects on six and four lipids, respectively. According to the HMDB database and KEGG pathways, nine important lipids were successfully matched to the involved biochemical pathways, such as glycerophospholipid metabolism, arachidonic acid metabolism, and linoleic acid metabolism. Therefore, IBU can cause disorders in the metabolism of the brain lipids of adult zebrafish and affect the composition of biological membranes, inflammatory responses, and cardiovascular and cerebrovascular diseases. The significant difference in the effects of R-(-)-IBU and S-(+)-IBU on lipidomics indicated that chiral IBU has stereoselective toxicity to aquatic organisms. Our study provided new insights into the environmental toxicology and highlighted the hazard of pharmaceutical and personal care product pollution in aquatic environments.


Assuntos
Peixe-Zebra , Animais , Encéfalo , Cromatografia Líquida de Alta Pressão , Ibuprofeno , Lipidômica
10.
Nat Commun ; 11(1): 375, 2020 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-31953382

RESUMO

Lipids play a pivotal role in biological processes and lipid analysis by mass spectrometry (MS) has significantly advanced lipidomic studies. While the structure specificity of lipid analysis proves to be critical for studying the biological functions of lipids, current mainstream methods for large-scale lipid analysis can only identify the lipid classes and fatty acyl chains, leaving the C=C location and sn-position unidentified. In this study, combining photochemistry and tandem MS we develop a simple but effective workflow to enable large-scale and near-complete lipid structure characterization with a powerful capability of identifying C=C location(s) and sn-position(s) simultaneously. Quantitation of lipid structure isomers at multiple levels of specificity is achieved and different subtypes of human breast cancer cells are successfully discriminated. Remarkably, human lung cancer tissues can only be distinguished from adjacent normal tissues using quantitative results of both lipid C=C location and sn-position isomers.


Assuntos
Lipidômica/métodos , Lipídeos/química , Animais , Neoplasias da Mama/química , Bovinos , Linhagem Celular Tumoral/química , Diabetes Mellitus Tipo 2/sangue , Escherichia coli/química , Glicerofosfolipídeos/análise , Glicerofosfolipídeos/química , Humanos , Isomerismo , Lipídeos/análise , Neoplasias Pulmonares/química , Fotoquímica , Plasma/química , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em Tandem
11.
Nat Commun ; 11(1): 331, 2020 01 16.
Artigo em Inglês | MEDLINE | ID: mdl-31949144

RESUMO

A comprehensive characterization of the lipidome from limited starting material remains very challenging. Here we report a high-sensitivity lipidomics workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS). Taking advantage of parallel accumulation-serial fragmentation (PASEF), we fragment on average 15 precursors in each of 100 ms TIMS scans, while maintaining the full mobility resolution of co-eluting isomers. The acquisition speed of over 100 Hz allows us to obtain MS/MS spectra of the vast majority of isotope patterns. Analyzing 1 µL of human plasma, PASEF increases the number of identified lipids more than three times over standard TIMS-MS/MS, achieving attomole sensitivity. Building on high intra- and inter-laboratory precision and accuracy of TIMS collisional cross sections (CCS), we compile 1856 lipid CCS values from plasma, liver and cancer cells. Our study establishes PASEF in lipid analysis and paves the way for sensitive, ion mobility-enhanced lipidomics in four dimensions.


Assuntos
Espectrometria de Mobilidade Iônica , Lipidômica/métodos , Lipídeos/sangue , Animais , Cromatografia Líquida , Análise de Dados , Humanos , Isomerismo , Isótopos , Camundongos , Espectrometria de Massas em Tandem , Fluxo de Trabalho
12.
J Agric Food Chem ; 68(7): 2240-2248, 2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-31975589

RESUMO

An electric soldering iron ion source (ESII) coupling with rapid evaporative ionization mass spectrometry (REIMS) was developed and used for in situ monitoring the dynamic variation trend in oxidation characteristics of fish oil during storage. The lipidomics profiles of fish oil stored at various days were acquired by ESII-REIMS. The fatty acid and triacylglycerol species were structurally identified, and their abundances were analyzed according to multivariate statistical models mainly including principle component analysis as well as orthogonal partial least-squares analysis. On the shared and unique structure plot, the ions of m/z 255.23, 281.24, 877.72, and 901.72 displayed the most significant variation among the oxidized fish oil samples. Based on receiver operating characteristic curve analysis with an optimal Youden index of 0.91, these markers were further verified. The variation of viscosity and volatiles were also evaluated to further verify the oxidation characteristics of fish oil. The study demonstrated that ESII-REIMS technology used as an advanced detection method could ensure fish oil quality during storage.


Assuntos
Óleos de Peixe/química , Lipidômica/métodos , Espectrometria de Massas/métodos , Ácidos Graxos/química , Armazenamento de Alimentos , Espectrometria de Massas/instrumentação , Oxirredução , Triglicerídeos/química , Viscosidade
13.
J Agric Food Chem ; 68(5): 1457-1467, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31931568

RESUMO

Antarctic krill oil (AKO) is susceptible to oxidation due to the high unsaturation degree of bioactive substances. Herein, a lipidomics method for in situ monitoring of the dynamic oxidation characteristics in AKO was explored based on electric soldering iron ion source (ESII) coupling with rapid evaporative ionization mass spectrometry (REIMS). The lipidomics profiles of AKO at different storage periods were successfully acquired. On the basis of principal component analysis and orthogonal partial least-squares analysis, the obtained REIMS data were employed to build a multivariate recognition model. The ions of m/z 707.50, 721.50, 833.49, and 837.54 contributed the most significant effect on the multivariate data model for the authentication of different AKO samples. Besides, the variation of viscosity, astaxanthin, and volatile compounds were also evaluated to corroborate the oxidation characteristics. The results indicated that the ESII-REIMS technology could be applied as an advanced rapid detection method to secure oil and fat quality during storage.


Assuntos
Euphausiacea/química , Lipidômica/métodos , Espectrometria de Massas/métodos , Óleos/química , Animais , Armazenamento de Alimentos , Oxirredução , Controle de Qualidade
14.
PLoS One ; 15(1): e0227455, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31914155

RESUMO

BACKGROUND: Multiple myeloma (MM) is a hematological malignancy characterized by the clonal expansion of malignant plasma cells. Though durable remissions are possible, MM is considered incurable, with relapse occurring in almost all patients. There has been limited data reported on the lipid metabolism changes in plasma cells during MM progression. Here, we evaluated the feasibility of concurrent lipidomics and proteomics analyses from patient plasma cells, and report these data on a limited number of patient samples, demonstrating the feasibility of the method, and establishing hypotheses to be evaluated in the future. METHODS: Plasma cells were purified from fresh bone marrow aspirates using CD138 microbeads. Proteins and lipids were extracted using a bi-phasic solvent system with methanol, methyl tert-butyl ether, and water. Untargeted proteomics, untargeted and targeted lipidomics were performed on 7 patient samples using liquid chromatography-mass spectrometry. Two comparisons were conducted: high versus low risk; relapse versus newly diagnosed. Proteins and pathways enriched in the relapsed group was compared to a public transcriptomic dataset from Multiple Myeloma Research Consortium reference collection (n = 222) at gene and pathways level. RESULTS: From one million purified plasma cells, we were able to extract material and complete untargeted (~6000 and ~3600 features in positive and negative mode respectively) and targeted lipidomics (313 lipids), as well as untargeted proteomics analysis (~4100 reviewed proteins). Comparative analyses revealed limited differences between high and low risk groups (according to the standard clinical criteria), hence we focused on drawing comparisons between the relapsed and newly diagnosed patients. Untargeted and targeted lipidomics indicated significant down-regulation of phosphatidylcholines (PCs) in relapsed MM. Although there was limited overlap of the differential proteins/transcripts, 76 significantly enriched pathways in relapsed MM were common between proteomics and transcriptomics data. Further evaluation of transcriptomics data for lipid metabolism network revealed enriched correlation of PC, ceramide, cardiolipin, arachidonic acid and cholesterol metabolism pathways to be exclusively correlated among relapsed but not in newly-diagnosed patients. CONCLUSIONS: This study establishes the feasibility and workflow to conduct integrated lipidomics and proteomics analyses on patient-derived plasma cells. Potential lipid metabolism changes associated with MM relapse warrant further investigation.


Assuntos
Lipídeos/análise , Mieloma Múltiplo/patologia , Plasmócitos/metabolismo , Proteoma/análise , Área Sob a Curva , Cromatografia Líquida de Alta Pressão , Humanos , Metabolismo dos Lipídeos , Lipidômica/métodos , Lipídeos/isolamento & purificação , Espectrometria de Massas , Mieloma Múltiplo/metabolismo , Fosfatidilcolinas/análise , Fosfatidilcolinas/metabolismo , Projetos Piloto , Plasmócitos/citologia , Proteoma/isolamento & purificação , Proteômica/métodos , Curva ROC , Recidiva , Transcriptoma
15.
PLoS One ; 15(1): e0228011, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31978172

RESUMO

There continues to be a significant shortage of donor livers for transplantation. One impediment is the discard rate of fatty, or steatotic, livers because of their poor post-transplant function. Steatotic livers are prone to significant ischemia-reperfusion injury (IRI) and data regarding how best to improve the quality of steatotic livers is lacking. Herein, we use normothermic (37°C) machine perfusion in combination with metabolic and lipidomic profiling to elucidate deficiencies in metabolic pathways in steatotic livers, and to inform strategies for improving their function. During perfusion, energy cofactors increased in steatotic livers to a similar extent as non-steatotic livers, but there were significant deficits in anti-oxidant capacity, efficient energy utilization, and lipid metabolism. Steatotic livers appeared to oxidize fatty acids at a higher rate but favored ketone body production rather than energy regeneration via the tricyclic acid cycle. As a result, lactate clearance was slower and transaminase levels were higher in steatotic livers. Lipidomic profiling revealed ω-3 polyunsaturated fatty acids increased in non-steatotic livers to a greater extent than in steatotic livers. The novel use of metabolic and lipidomic profiling during ex situ normothermic machine perfusion has the potential to guide the resuscitation and rehabilitation of steatotic livers for transplantation.


Assuntos
Fígado Gorduroso/metabolismo , Lipidômica , Metabolômica , Perfusão , Ressuscitação , Temperatura , Trifosfato de Adenosina/biossíntese , Ácidos e Sais Biliares/metabolismo , Ácidos Graxos/metabolismo , Fígado Gorduroso/patologia , Fígado Gorduroso/fisiopatologia , Glucose/metabolismo , Hemodinâmica , Humanos , Fígado/patologia , Fígado/fisiopatologia , Testes de Função Hepática , Oxirredução , Estresse Oxidativo , Resistência Vascular
16.
PLoS One ; 15(1): e0228010, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31999740

RESUMO

Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.


Assuntos
Colo/patologia , Neoplasias do Colo/metabolismo , Neoplasias do Colo/patologia , Células Epiteliais/metabolismo , Lipidômica , Fosfolipídeos/metabolismo , Linhagem Celular Tumoral , Células Epiteliais/patologia , Humanos , Análise de Componente Principal
17.
J Chromatogr A ; 1618: 460849, 2020 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-31928769

RESUMO

Aging refers to the intracellular accumulation of reactive oxygen species that damages proteins, DNA, and lipids. As alterations in lipid metabolism may trigger metabolic disorders and the onset of metabolic diseases, changes in lipid profiles can be closely related to aging. In this study, a comprehensive lipidomic comparison between 4- and 25-month-old mice was performed to investigate age-induced changes in the lipid profiles of mouse serum, kidney, and heart using nanoflow ultrahigh-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Quantitative analysis of 279 of the 542 identified lipids revealed significant changes upon aging, mainly showing decreased levels in the three types of samples. Exceptionally, most triacylglycerols showed significant increases in heart tissue. The kidney was influenced more by aging than the serum and heart. The highly abundant lipids in each lipid class with significant decreases (> 2-fold, p < 0.01) were lysophosphatidic acid 18:1, lysophosphatidylinositol 20:4, and ceramide d:18:1/24:0 in serum; lysophosphatidylglycerol 16:0 in heart tissue; and eight phosphatidylethanolamines (20:4, 22:6, 36:2, 36:3, 38:4, 38:5, 38:6, 40:6, and 40:7), two cardiolipins (72:7 and 72:8), and lysophosphatidylcholine 18:0 in kidney tissue. The findings indicate the potential of lipidomic analysis to study characteristic age-related lipid changes.


Assuntos
Envelhecimento/metabolismo , Rim/metabolismo , Lipidômica , Lipídeos/sangue , Miocárdio/metabolismo , Nanotecnologia , Reologia , Espectrometria de Massas em Tandem/métodos , Animais , Cromatografia Líquida de Alta Pressão , Feminino , Metabolismo dos Lipídeos , Lipídeos/análise , Camundongos Endogâmicos C57BL , Análise de Componente Principal , Padrões de Referência , Espectrometria de Massas por Ionização por Electrospray/métodos
18.
Talanta ; 209: 120593, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31892006

RESUMO

The impact of preanalytical sample handling on lipid stability has been assessed in human plasma using targeted LC-MS/MS quantification of endocannabinoids, sphingolipids and LPA, complemented by non-targeted lipidomics screening with LC-QTOFMS. The study involved incubation of whole blood and plasma from healthy volunteers at room temperature or in ice water for time periods ranging from 20 min to 24 h. The impact of two different anticoagulants, K3EDTA and sodium fluoride/citrate, on lipid stability was evaluated. It was found that the concentrations determined for several endogenous lipids vary when whole blood and plasma samples are processed at room temperature, whereas the concentrations of most lipids were stable for 4 h in ice water. Surprisingly, the detected amounts of endocannabinoids 1- and 2-arachidonoyl glycerol and arachidonoyl ethanolamide increased markedly by 60, 95, and 30% in K3EDTA whole blood after storage in ice water for only 20 min. When using sodium fluoride/citrate blood collection tubes, the stability of several lipids, including that of the endocannabinoids, was improved. Accordingly, it is absolutely necessary to keep the blood sampling and plasma processing time below 1 h to avoid ex-vivo formation of endocannabinoids. It is worth mentioning that baseline lipid levels differ when using K3EDTA or sodium fluoride/citrate blood sampling tubes, which emphasizes the importance of traceability of reported plasma concentrations to the used anticoagulant.


Assuntos
Coleta de Amostras Sanguíneas/métodos , Endocanabinoides/sangue , Lipidômica/métodos , Lisofosfolipídeos/sangue , Esfingolipídeos/sangue , Adulto , Cromatografia Líquida/métodos , Ácido Cítrico/química , Feminino , Fluoretos/química , Humanos , Masculino , Espectrometria de Massas/métodos
19.
Phytomedicine ; 67: 153162, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31955134

RESUMO

BACKGROUND: Salvia miltiorrhiza and Panax notoginseng herb pair (DQ) has been widely used in traditional Chinese medicine for a long history to prevent and treat the coronary heart disease. However, its protective mechanisms against myocardial ischemia during coronary heart disease remain not well-understood. PURPOSE: In this study, we aimed to explore the protective mechanisms of DQ on myocardial ischemia from the perspective of serum lipidomics and amino acids (AAs). METHODS: Rats were orally administrated with low-dose DQ (L-DQ, 0.24 g/kg) and high-dose DQ (H-DQ, 0.96 g/kg) for two weeks and subcutaneously injected with isoproterenol (ISO, 65 mg/kg) for two consecutive days (13th and 14th days) to induce acute myocardial ischemia (AMI). Heart histopathology and serum biochemical indices were examined. The specifically altered serum lipid metabolites were profiled via lipidomics approach, while serum AA profiles were analyzed using UHPLC-TQ-MS/MS. RESULTS: Cardiac marker enzymes (CK, CK-MB, LDH and cTn-I) were significantly upregulated in AMI rats with some of which significantly dropped to normal level in L- and H-DQ groups. Serum TC, TG, HDL, LDL, VLDL and FFA were improved in AMI rats treatment with L- and H-DQ. Further, the PCA based on lipidomics showed serum lipid metabolites in L- and H-DQ groups were closer to control group than that in model group. Compared with model group, H-DQ pretreatment significantly reduced SM (d34:1) and CE (20:4), and increased FA (20:5), PC (26:1), TG (56:9), TG (54:7), MG (17:0), Cer (d32:0) and Cer (d34:0), whereas L-DQ significantly alleviated the perturbed levels of CE (20:4), FA (20:5), MG (17:0), and SM (d34:1). Moreover, there was a significant increment for leucine, isoleucine, valine, phenylalanine, lysine and glutamate but a significant reduction for tryptophan in the serum of rats in model group as compared to control group. Intriguingly, H-DQ could significantly decrease the levels of glutamate, lysine, isoleucine, and BCAAs (the sum of leucine, isoleucine and valine) after AMI, while L-DQ had no significant effects on the above altered AAs. The Western blotting results implied that H-DQ could promote the myocardial BCAA catabolism in AMI rats by activation of BCKDHA, whereas by inhibition of BCKDHK. CONCLUSION: This study presents evidence for the therapeutic effects of DQ on AMI injury, in part, via co-regulating lipid and AA metabolisms.


Assuntos
Aminoácidos/sangue , Medicamentos de Ervas Chinesas/farmacologia , Lipídeos/sangue , Isquemia Miocárdica/tratamento farmacológico , Panax notoginseng/química , Salvia miltiorrhiza/química , Animais , Cardiotônicos/farmacologia , Cromatografia Líquida de Alta Pressão , Coração/efeitos dos fármacos , Lipidômica/métodos , Masculino , Isquemia Miocárdica/metabolismo , Miocárdio/patologia , Ratos Sprague-Dawley , Espectrometria de Massas em Tandem
20.
J Dairy Sci ; 103(2): 1900-1907, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31785883

RESUMO

Triglyceride (TG) and fatty acid profiles of raw (RM), pasteurized (PM, 85°C for 15 s), and indirect UHT-treated (UM, 135°C for 15 s) cow milk were investigated by a lipidomics approach. Ninety-four TG were identified and all were present at significantly lower concentrations in UM than in RM or PM, and free fatty acid contents were significantly higher in UM than in RM and PM, indicating that TG lipolysis occurred to a greater degree in UM than in RM and PM. In addition, UM contained significantly fewer unsaturated fatty acids (14 types) than those in RM and PM, including C14:1n-5, C15:1n-5, C16:1n-7, C17:1n-7, C18:1n9 cis, C18:2n-6 cis, C18:3n-3, C18:3n-6, C20:1, C20:2, C20:3n-6, C20:3n-3, C20:4n-6, and C20:5n-3. However, we detected no significant differences between RM and PM in these fatty acids. In conclusion, UHT treatment, but not pasteurization, caused loss of the nutritional quality and bioactivity of cow milk lipid profiles.


Assuntos
Bovinos/fisiologia , Ácidos Graxos/análise , Lipídeos/análise , Leite/química , Animais , Ácidos Graxos Insaturados/análise , Feminino , Temperatura Alta , Lipidômica , Lipólise , Valor Nutritivo , Pasteurização
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