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1.
Nat Commun ; 12(1): 4839, 2021 08 10.
Artigo em Inglês | MEDLINE | ID: mdl-34376673

RESUMO

The ability to maintain a sequence of items in memory is a fundamental cognitive function. In the rodent hippocampus, the representation of sequentially organized spatial locations is reflected by the phase of action potentials relative to the theta oscillation (phase precession). We investigated whether the timing of neuronal activity relative to the theta brain oscillation also reflects sequence order in the medial temporal lobe of humans. We used a task in which human participants learned a fixed sequence of pictures and recorded single neuron and local field potential activity with implanted electrodes. We report that spikes for three consecutive items in the sequence (the preferred stimulus for each cell, as well as the stimuli immediately preceding and following it) were phase-locked at distinct phases of the theta oscillation. Consistent with phase precession, spikes were fired at progressively earlier phases as the sequence advanced. These findings generalize previous findings in the rodent hippocampus to the human temporal lobe and suggest that encoding stimulus information at distinct oscillatory phases may play a role in maintaining sequential order in memory.


Assuntos
Potenciais de Ação/fisiologia , Epilepsia/fisiopatologia , Aprendizagem/fisiologia , Neurônios/fisiologia , Ritmo Teta/fisiologia , Adolescente , Adulto , Epilepsia/diagnóstico , Feminino , Hipocampo/citologia , Hipocampo/fisiologia , Humanos , Masculino , Modelos Neurológicos , Neurônios/citologia , Estimulação Luminosa/métodos , Lobo Temporal/citologia , Lobo Temporal/fisiologia , Adulto Jovem
2.
Science ; 373(6554): 581-585, 2021 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-34210891

RESUMO

The question of how the brain recognizes the faces of familiar individuals has been important throughout the history of neuroscience. Cells linking visual processing to person memory have been proposed but not found. Here, we report the discovery of such cells through recordings from an area in the macaque temporal pole identified with functional magnetic resonance imaging. These cells responded to faces that were personally familiar. They responded nonlinearly to stepwise changes in face visibility and detail and holistically to face parts, reflecting key signatures of familiar face recognition. They discriminated between familiar identities, as fast as a general face identity area. The discovery of these cells establishes a new pathway for the fast recognition of familiar individuals.


Assuntos
Reconhecimento Facial , Memória , Neurônios/fisiologia , Lobo Temporal/fisiologia , Animais , Mapeamento Encefálico , Face , Macaca mulatta , Imageamento por Ressonância Magnética , Masculino , Lobo Temporal/citologia , Percepção Visual
3.
Neuron ; 109(17): 2781-2796.e10, 2021 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-34265253

RESUMO

Spatial navigation and memory rely on neural systems that encode places, distances, and directions in relation to the external world or relative to the navigating organism. Place, grid, and head-direction cells form key units of world-referenced, allocentric cognitive maps, but the neural basis of self-centered, egocentric representations remains poorly understood. Here, we used human single-neuron recordings during virtual spatial navigation tasks to identify neurons providing a neural code for egocentric spatial maps in the human brain. Consistent with previous observations in rodents, these neurons represented egocentric bearings toward reference points positioned throughout the environment. Egocentric bearing cells were abundant in the parahippocampal cortex and supported vectorial representations of egocentric space by also encoding distances toward reference points. Beyond navigation, the observed neurons showed activity increases during spatial and episodic memory recall, suggesting that egocentric bearing cells are not only relevant for navigation but also play a role in human memory.


Assuntos
Memória Episódica , Neurônios/fisiologia , Memória Espacial , Lobo Temporal/fisiologia , Adulto , Eletroencefalografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Navegação Espacial , Lobo Temporal/citologia
4.
Int J Mol Sci ; 22(13)2021 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-34206571

RESUMO

In recent years, a large population of immature neurons has been documented in the paralaminar nucleus of the primate amygdala. A substantial fraction of these immature neurons differentiate into mature neurons during postnatal development or following selective lesion of the hippocampus. Notwithstanding a growing number of studies on the origin and fate of these immature neurons, fundamental questions about the life and death of these neurons remain. Here, we briefly summarize what is currently known about the immature neurons present in the primate ventral amygdala during development and in adulthood, as well as following selective hippocampal lesions. We provide evidence confirming that the distribution of immature neurons extends to the anterior portions of the entorhinal cortex and layer II of the perirhinal cortex. We also provide novel arguments derived from stereological estimates of the number of mature and immature neurons, which support the view that the migration of immature neurons from the lateral ventricle accompanies neuronal maturation in the primate amygdala at all ages. Finally, we propose and discuss the hypothesis that increased migration and maturation of neurons in the amygdala following hippocampal dysfunction may be linked to behavioral alterations associated with certain neurodevelopmental disorders.


Assuntos
Tonsila do Cerebelo/citologia , Diferenciação Celular , Neurônios/citologia , Neurônios/metabolismo , Fatores Etários , Tonsila do Cerebelo/metabolismo , Animais , Biomarcadores , Contagem de Células , Morte Celular , Sobrevivência Celular , Expressão Gênica , Hipocampo/citologia , Hipocampo/metabolismo , Imuno-Histoquímica , Primatas , Lobo Temporal/citologia , Lobo Temporal/metabolismo
5.
Nat Commun ; 12(1): 1103, 2021 02 17.
Artigo em Inglês | MEDLINE | ID: mdl-33597516

RESUMO

Neurons in some sensory areas reflect the content of working memory (WM) in their spiking activity. However, this spiking activity is seldom related to behavioral performance. We studied the responses of inferotemporal (IT) neurons, which exhibit object-selective activity, along with Frontal Eye Field (FEF) neurons, which exhibit spatially selective activity, during the delay period of an object WM task. Unlike the spiking activity and local field potentials (LFPs) within these areas, which were poor predictors of behavioral performance, the phase-locking of IT spikes and LFPs with the beta band of FEF LFPs robustly predicted successful WM maintenance. In addition, IT neurons exhibited greater object-selective persistent activity when their spikes were locked to the phase of FEF LFPs. These results reveal that the coordination between prefrontal and temporal cortex predicts the successful maintenance of visual information during WM.


Assuntos
Macaca mulatta/fisiologia , Memória de Curto Prazo/fisiologia , Neurônios/fisiologia , Córtex Pré-Frontal/fisiologia , Lobo Temporal/fisiologia , Potenciais de Ação/fisiologia , Algoritmos , Animais , Lobo Frontal/citologia , Lobo Frontal/fisiologia , Masculino , Modelos Neurológicos , Estimulação Luminosa , Córtex Pré-Frontal/citologia , Lobo Temporal/citologia
6.
J Neurosci ; 41(15): 3386-3399, 2021 04 14.
Artigo em Inglês | MEDLINE | ID: mdl-33431634

RESUMO

Research in functional neuroimaging has suggested that category-selective regions of visual cortex, including the ventral temporal cortex (VTC), can be reactivated endogenously through imagery and recall. Face representation in the monkey face-patch system has been well studied and is an attractive domain in which to explore these processes in humans. The VTCs of 8 human subjects (4 female) undergoing invasive monitoring for epilepsy surgery were implanted with microelectrodes. Most (26 of 33) category-selective units showed specificity for face stimuli. Different face exemplars evoked consistent and discriminable responses in the population of units sampled. During free recall, face-selective units preferentially reactivated in the absence of visual stimulation during a 2 s window preceding face recall events. Furthermore, we show that in at least 1 subject, the identity of the recalled face could be predicted by comparing activity preceding recall events to activity evoked by visual stimulation. We show that face-selective units in the human VTC are reactivated endogenously, and present initial evidence that consistent representations of individual face exemplars are specifically reactivated in this manner.SIGNIFICANCE STATEMENT The role of "top-down" endogenous reactivation of native representations in higher sensory areas is poorly understood in humans. We conducted the first detailed single-unit survey of ventral temporal cortex (VTC) in human subjects, showing that, similarly to nonhuman primates, humans encode different faces using different rate codes. Then, we demonstrated that, when subjects recalled and imagined a given face, VTC neurons reactivated with the same rate codes as when subjects initially viewed that face. This suggests that the VTC units not only carry durable representations of faces, but that those representations can be endogenously reactivated via "top-down" mechanisms.


Assuntos
Reconhecimento Facial , Lobo Temporal/fisiologia , Adulto , Potenciais Evocados Visuais , Feminino , Humanos , Masculino , Rememoração Mental , Pessoa de Meia-Idade , Neurônios/fisiologia , Lobo Temporal/citologia
7.
J Neurosci ; 41(5): 883-890, 2021 02 03.
Artigo em Inglês | MEDLINE | ID: mdl-33257323

RESUMO

Deciphering the mechanisms of human memory is a central goal of neuroscience, both from the point of view of the fundamental biology of memory and for its translational relevance. Here, we review some contributions that recordings from neurons in humans implanted with electrodes for clinical purposes have made toward this goal. Recordings from the medial temporal lobe, including the hippocampus, reveal the existence of two classes of cells: those encoding highly selective and invariant representations of abstract concepts, and memory-selective cells whose activity is related to familiarity and episodic retrieval. Insights derived from observing these cells in behaving humans include that semantic representations are activated before episodic representations, that memory content and memory strength are segregated, and that the activity of both types of cells is related to subjective awareness as expected from a substrate for declarative memory. Visually selective cells can remain persistently active for several seconds, thereby revealing a cellular substrate for working memory in humans. An overarching insight is that the neural code of human memory is interpretable at the single-neuron level. Jointly, intracranial recording studies are starting to reveal aspects of the building blocks of human memory at the single-cell level. This work establishes a bridge to cellular-level work in animals on the one hand, and the extensive literature on noninvasive imaging in humans on the other hand. More broadly, this work is a step toward a detailed mechanistic understanding of human memory that is needed to develop therapies for human memory disorders.


Assuntos
Eletrodos Implantados , Hipocampo/fisiologia , Memória Episódica , Memória de Curto Prazo/fisiologia , Neurônios/fisiologia , Lobo Temporal/fisiologia , Hipocampo/citologia , Humanos , Transtornos da Memória/diagnóstico , Transtornos da Memória/fisiopatologia , Rememoração Mental/fisiologia , Lobo Temporal/citologia
8.
Elife ; 92020 12 29.
Artigo em Inglês | MEDLINE | ID: mdl-33372656

RESUMO

The advancement of single-cell RNA-sequencing technologies has led to an explosion of cell type definitions across multiple organs and organisms. While standards for data and metadata intake are arising, organization of cell types has largely been left to individual investigators, resulting in widely varying nomenclature and limited alignment between taxonomies. To facilitate cross-dataset comparison, the Allen Institute created the common cell type nomenclature (CCN) for matching and tracking cell types across studies that is qualitatively similar to gene transcript management across different genome builds. The CCN can be readily applied to new or established taxonomies and was applied herein to diverse cell type datasets derived from multiple quantifiable modalities. The CCN facilitates assigning accurate yet flexible cell type names in the mammalian cortex as a step toward community-wide efforts to organize multi-source, data-driven information related to cell type taxonomies from any organism.


Assuntos
Células/classificação , Lobo Temporal/citologia , Terminologia como Assunto , Humanos
9.
Nat Commun ; 11(1): 5038, 2020 10 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028830

RESUMO

Epilepsy is one of the most common neurological disorders, yet its pathophysiology is poorly understood due to the high complexity of affected neuronal circuits. To identify dysfunctional neuronal subtypes underlying seizure activity in the human brain, we have performed single-nucleus transcriptomics analysis of >110,000 neuronal transcriptomes derived from temporal cortex samples of multiple temporal lobe epilepsy and non-epileptic subjects. We found that the largest transcriptomic changes occur in distinct neuronal subtypes from several families of principal neurons (L5-6_Fezf2 and L2-3_Cux2) and GABAergic interneurons (Sst and Pvalb), whereas other subtypes in the same families were less affected. Furthermore, the subtypes with the largest epilepsy-related transcriptomic changes may belong to the same circuit, since we observed coordinated transcriptomic shifts across these subtypes. Glutamate signaling exhibited one of the strongest dysregulations in epilepsy, highlighted by layer-wise transcriptional changes in multiple glutamate receptor genes and strong upregulation of genes coding for AMPA receptor auxiliary subunits. Overall, our data reveal a neuronal subtype-specific molecular phenotype of epilepsy.


Assuntos
Epilepsia Resistente a Medicamentos/genética , Epilepsia do Lobo Temporal/genética , Neurônios/patologia , Lobo Temporal/patologia , Transcriptoma/genética , Adolescente , Adulto , Biópsia , Estudos de Casos e Controles , Núcleo Celular/genética , Núcleo Celular/metabolismo , Conjuntos de Dados como Assunto , Epilepsia Resistente a Medicamentos/diagnóstico , Epilepsia Resistente a Medicamentos/patologia , Epilepsia Resistente a Medicamentos/cirurgia , Epilepsia do Lobo Temporal/diagnóstico , Epilepsia do Lobo Temporal/patologia , Epilepsia do Lobo Temporal/cirurgia , Feminino , Ácido Glutâmico/metabolismo , Humanos , Imageamento por Ressonância Magnética , Masculino , Microdissecção , Pessoa de Meia-Idade , Modelos Genéticos , Rede Nervosa/metabolismo , Rede Nervosa/patologia , Neurônios/citologia , Neurônios/metabolismo , RNA-Seq , Receptores de AMPA/genética , Receptores de AMPA/metabolismo , Receptores de Glutamato/genética , Receptores de Glutamato/metabolismo , Transdução de Sinais/genética , Análise de Célula Única , Lobo Temporal/citologia , Lobo Temporal/diagnóstico por imagem , Lobo Temporal/cirurgia , Transcrição Genética , Regulação para Cima , Adulto Jovem
10.
Biol Psychol ; 154: 107926, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32621851

RESUMO

Unpleasant, compared to neutral, scenes reliably prompt enhanced functional brain activity in the amygdala and inferotemporal cortex. Considering data from psychophysiological studies in which defensive reactivity is further enhanced when viewing unpleasant scenes under threat of shock (compared to safety), the current study investigates functional activation in the amygdala-inferotemporal circuit when unpleasant (or neutral) scenes are viewed under threat of shock or safety. In this paradigm, a cue signaling threat or safety was presented in conjunction with either an unpleasant or neutral picture. Replicating previous studies, unpleasant, compared to neutral, scenes reliably enhanced activation in the amygdala and inferotemporal cortex. Functional activity in these regions, however, did not differ whether scenes were presented in a context threatening shock exposure, compared to safety, which instead activated regions of the anterior insula and cingulate cortex. Taken together, the data support a view in which neural regions activated in different defensive situations act independently.


Assuntos
Aprendizagem da Esquiva/fisiologia , Córtex Cerebral/citologia , Córtex Cerebral/fisiologia , Neurônios/fisiologia , Adolescente , Tonsila do Cerebelo/citologia , Tonsila do Cerebelo/fisiologia , Estimulação Elétrica , Feminino , Giro do Cíngulo/citologia , Giro do Cíngulo/fisiologia , Humanos , Lobo Temporal/citologia , Lobo Temporal/fisiologia , Córtex Visual/citologia , Córtex Visual/fisiologia
11.
Nature ; 583(7814): 103-108, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32494012

RESUMO

The inferotemporal (IT) cortex is responsible for object recognition, but it is unclear how the representation of visual objects is organized in this part of the brain. Areas that are selective for categories such as faces, bodies, and scenes have been found1-5, but large parts of IT cortex lack any known specialization, raising the question of what general principle governs IT organization. Here we used functional MRI, microstimulation, electrophysiology, and deep networks to investigate the organization of macaque IT cortex. We built a low-dimensional object space to describe general objects using a feedforward deep neural network trained on object classification6. Responses of IT cells to a large set of objects revealed that single IT cells project incoming objects onto specific axes of this space. Anatomically, cells were clustered into four networks according to the first two components of their preferred axes, forming a map of object space. This map was repeated across three hierarchical stages of increasing view invariance, and cells that comprised these maps collectively harboured sufficient coding capacity to approximately reconstruct objects. These results provide a unified picture of IT organization in which category-selective regions are part of a coarse map of object space whose dimensions can be extracted from a deep network.


Assuntos
Modelos Neurológicos , Percepção Espacial/fisiologia , Lobo Temporal/citologia , Lobo Temporal/fisiologia , Animais , Estimulação Elétrica , Macaca mulatta/fisiologia , Imageamento por Ressonância Magnética , Masculino , Vias Neurais/fisiologia , Lobo Temporal/anatomia & histologia
12.
Nat Commun ; 11(1): 3192, 2020 06 24.
Artigo em Inglês | MEDLINE | ID: mdl-32581214

RESUMO

Resolving approach-avoidance conflicts relies on encoding motivation outcomes and learning from past experiences. Accumulating evidence points to the role of the Medial Temporal Lobe (MTL) and Medial Prefrontal Cortex (mPFC) in these processes, but their differential contributions have not been convincingly deciphered in humans. We detect 310 neurons from mPFC and MTL from patients with epilepsy undergoing intracranial recordings and participating in a goal-conflict task where rewards and punishments could be controlled or not. mPFC neurons are more selective to punishments than rewards when controlled. However, only MTL firing following punishment is linked to a lower probability for subsequent approach behavior. mPFC response to punishment precedes a similar MTL response and affects subsequent behavior via an interaction with MTL firing. We thus propose a model where approach-avoidance conflict resolution in humans depends on outcome value tagging in mPFC neurons influencing encoding of such value in MTL to affect subsequent choice.


Assuntos
Comportamento de Escolha/fisiologia , Objetivos , Neurônios/fisiologia , Córtex Pré-Frontal/fisiologia , Lobo Temporal/fisiologia , Adolescente , Adulto , Idoso , Mapeamento Encefálico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Modelos Neurológicos , Córtex Pré-Frontal/citologia , Punição , Tempo de Reação , Recompensa , Lobo Temporal/citologia , Adulto Jovem
13.
Mol Cell ; 79(3): 521-534.e15, 2020 08 06.
Artigo em Inglês | MEDLINE | ID: mdl-32592681

RESUMO

Genome-wide mapping of chromatin interactions at high resolution remains experimentally and computationally challenging. Here we used a low-input "easy Hi-C" protocol to map the 3D genome architecture in human neurogenesis and brain tissues and also demonstrated that a rigorous Hi-C bias-correction pipeline (HiCorr) can significantly improve the sensitivity and robustness of Hi-C loop identification at sub-TAD level, especially the enhancer-promoter (E-P) interactions. We used HiCorr to compare the high-resolution maps of chromatin interactions from 10 tissue or cell types with a focus on neurogenesis and brain tissues. We found that dynamic chromatin loops are better hallmarks for cellular differentiation than compartment switching. HiCorr allowed direct observation of cell-type- and differentiation-specific E-P aggregates spanning large neighborhoods, suggesting a mechanism that stabilizes enhancer contacts during development. Interestingly, we concluded that Hi-C loop outperforms eQTL in explaining neurological GWAS results, revealing a unique value of high-resolution 3D genome maps in elucidating the disease etiology.


Assuntos
Cromatina/metabolismo , Elementos Facilitadores Genéticos , Regulação da Expressão Gênica no Desenvolvimento , Redes Reguladoras de Genes , Genoma Humano , Neurogênese/genética , Regiões Promotoras Genéticas , Adulto , Linhagem Celular , Cérebro/citologia , Cérebro/crescimento & desenvolvimento , Cérebro/metabolismo , Cromatina/ultraestrutura , Mapeamento Cromossômico , Feto , Histonas/genética , Histonas/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Proteínas do Tecido Nervoso/classificação , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Células-Tronco Neurais/citologia , Células-Tronco Neurais/metabolismo , Doenças Neurodegenerativas/genética , Doenças Neurodegenerativas/metabolismo , Doenças Neurodegenerativas/patologia , Neurônios/citologia , Neurônios/metabolismo , Lobo Temporal/citologia , Lobo Temporal/crescimento & desenvolvimento , Lobo Temporal/metabolismo , Fatores de Transcrição/classificação , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
14.
Neuron ; 107(3): 566-579.e7, 2020 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-32473095

RESUMO

Mother-infant bonding develops rapidly following parturition and is accompanied by changes in sensory perception and behavior. Here, we study how ultrasonic vocalizations (USVs) are represented in the brain of mothers. Using a mouse line that allows temporally controlled genetic access to active neurons, we find that the temporal association cortex (TeA) in mothers exhibits robust USV responses. Rabies tracing from USV-responsive neurons reveals extensive subcortical and cortical inputs into TeA. A particularly dominant cortical source of inputs is the primary auditory cortex (A1), suggesting strong A1-to-TeA connectivity. Chemogenetic silencing of USV-responsive neurons in TeA impairs auditory-driven maternal preference in a pup-retrieval assay. Furthermore, dense extracellular recordings from awake mice reveal changes of both single-neuron and population responses to USVs in TeA, improving discriminability of pup calls in mothers compared with naive females. These data indicate that TeA plays a key role in encoding and perceiving pup cries during motherhood.


Assuntos
Córtex Auditivo/fisiologia , Percepção Auditiva/fisiologia , Comportamento Materno , Plasticidade Neuronal/fisiologia , Neurônios/fisiologia , Lobo Temporal/fisiologia , Vocalização Animal , Animais , Córtex Auditivo/citologia , Fenômenos Eletrofisiológicos , Feminino , Camundongos , Vias Neurais , Apego ao Objeto , Lobo Temporal/citologia , Ondas Ultrassônicas
15.
PLoS One ; 15(4): e0230666, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32271773

RESUMO

Although the connection between the left inferior frontal gyrus (LIFG) and the left superior temporal gyrus (LSTG) has been found to be essential for the comprehension of relative clause (RC) sentences, it remains unclear how the LIFG and the LSTG interact with each other, especially during the processing of Chinese RC sentences with different processing difficulty. This study thus conducted a 2 × 2 (modifying position × extraction position) factorial analyses to examine how these two factors influences regional brain activation. The results showed that, regardless of the modifying position, greater activation in the LIFG was consistently elicited in Chinese subject-extracted relative clauses (SRCs) with non-canonical word order than object-extracted relative clauses (ORCs) with canonical word order, implying that the LIFG subserving the ordering process primarily contributes to the processing of information with increased integration demands due to the non-canonical sequence. Moreover, the directional connection between the LIFG and the LSTG appeared to be modulated by different modifying positions. When the RC was at the subject-modifying position, the effective connectivity from the LIFG to the LSTG was dominantly activated for sentence comprehension; whereas when the RC was at the object-modifying position thus being more difficult, it might be the feedback mechanism from the LSTG back to the LIFG that took place in sentence processing. These findings reveal that brain activation in between the LIFG and the LSTG may be dynamically modulated by different processing difficulty and suggest the relative specialization but extensive collaboration involved in the LIFG and the LSTG for sentence comprehension.


Assuntos
Compreensão/fisiologia , Idioma , Rede Nervosa/fisiologia , Córtex Pré-Frontal/citologia , Leitura , Lobo Temporal/citologia , Adulto , Grupo com Ancestrais do Continente Asiático , Mapeamento Encefálico , Feminino , Humanos , Linguística , Imageamento por Ressonância Magnética , Masculino , Rede Nervosa/anatomia & histologia , Rede Nervosa/diagnóstico por imagem , Córtex Pré-Frontal/anatomia & histologia , Córtex Pré-Frontal/diagnóstico por imagem , Córtex Pré-Frontal/fisiologia , Lobo Temporal/anatomia & histologia , Lobo Temporal/diagnóstico por imagem , Lobo Temporal/fisiologia , Adulto Jovem
16.
Sci Data ; 7(1): 78, 2020 03 04.
Artigo em Inglês | MEDLINE | ID: mdl-32132545

RESUMO

A challenge for data sharing in systems neuroscience is the multitude of different data formats used. Neurodata Without Borders: Neurophysiology 2.0 (NWB:N) has emerged as a standardized data format for the storage of cellular-level data together with meta-data, stimulus information, and behavior. A key next step to facilitate NWB:N adoption is to provide easy to use processing pipelines to import/export data from/to NWB:N. Here, we present a NWB-formatted dataset of 1863 single neurons recorded from the medial temporal lobes of 59 human subjects undergoing intracranial monitoring while they performed a recognition memory task. We provide code to analyze and export/import stimuli, behavior, and electrophysiological recordings to/from NWB in both MATLAB and Python. The data files are NWB:N compliant, which affords interoperability between programming languages and operating systems. This combined data and code release is a case study for how to utilize NWB:N for human single-neuron recordings and enables easy re-use of this hard-to-obtain data for both teaching and research on the mechanisms of human memory.


Assuntos
Disseminação de Informação , Armazenamento e Recuperação da Informação/normas , Memória , Neurônios/fisiologia , Fenômenos Eletrofisiológicos , Humanos , Software , Lobo Temporal/citologia
17.
Nat Commun ; 11(1): 1172, 2020 03 03.
Artigo em Inglês | MEDLINE | ID: mdl-32127543

RESUMO

von Economo neurons (VENs) are bipolar, spindle-shaped neurons restricted to layer 5 of human frontoinsula and anterior cingulate cortex that appear to be selectively vulnerable to neuropsychiatric and neurodegenerative diseases, although little is known about other VEN cellular phenotypes. Single nucleus RNA-sequencing of frontoinsula layer 5 identifies a transcriptomically-defined cell cluster that contained VENs, but also fork cells and a subset of pyramidal neurons. Cross-species alignment of this cell cluster with a well-annotated mouse classification shows strong homology to extratelencephalic (ET) excitatory neurons that project to subcerebral targets. This cluster also shows strong homology to a putative ET cluster in human temporal cortex, but with a strikingly specific regional signature. Together these results suggest that VENs are a regionally distinctive type of ET neuron. Additionally, we describe the first patch clamp recordings of VENs from neurosurgically-resected tissue that show distinctive intrinsic membrane properties relative to neighboring pyramidal neurons.


Assuntos
Neurônios/fisiologia , Lobo Temporal/citologia , Transcriptoma , Animais , Encéfalo/citologia , Encéfalo/fisiologia , Eletrofisiologia/métodos , Perfilação da Expressão Gênica , Humanos , Hibridização in Situ Fluorescente , Camundongos , Neurônios/citologia , Células Piramidais/fisiologia , Telencéfalo/citologia , Lobo Temporal/fisiologia
18.
Nat Commun ; 11(1): 960, 2020 02 19.
Artigo em Inglês | MEDLINE | ID: mdl-32075960

RESUMO

The functional organization of the hippocampus is distributed as a gradient along its longitudinal axis that explains its differential interaction with diverse brain systems. We show that the location of human tissue samples extracted along the longitudinal axis of the adult human hippocampus can be predicted within 2mm using the expression pattern of less than 100 genes. Futhermore, this model generalizes to an external set of tissue samples from prenatal human hippocampi. We examine variation in this specific gene expression pattern across the whole brain, finding a distinct anterioventral-posteriodorsal gradient. We find frontal and anterior temporal regions involved in social and motivational behaviors, and more functionally connected to the anterior hippocampus, to be clearly differentiated from posterior parieto-occipital regions involved in visuospatial cognition and more functionally connected to the posterior hippocampus. These findings place the human hippocampus at the interface of two major brain systems defined by a single molecular gradient.


Assuntos
Conectoma , Perfilação da Expressão Gênica , Hipocampo/fisiologia , Rede Nervosa/fisiologia , Encéfalo/metabolismo , Encéfalo/fisiologia , Expressão Gênica , Hipocampo/citologia , Hipocampo/metabolismo , Humanos , Modelos Neurológicos , Rede Nervosa/metabolismo , Vias Neurais/citologia , Vias Neurais/metabolismo , Vias Neurais/fisiologia , Doenças Neurodegenerativas/genética , Doenças Neurodegenerativas/metabolismo , Doenças Neurodegenerativas/patologia , Neurônios/fisiologia , Lobo Temporal/citologia , Lobo Temporal/metabolismo , Lobo Temporal/fisiologia
19.
Sci Data ; 7(1): 30, 2020 01 21.
Artigo em Inglês | MEDLINE | ID: mdl-31964868

RESUMO

We present an electrophysiological dataset recorded from nine subjects during a verbal working memory task. Subjects were epilepsy patients undergoing intracranial monitoring for the localization of epileptic seizures. Subjects performed a modified Sternberg task in which the encoding of memory items, maintenance, and recall were temporally separated. The dataset includes simultaneously recorded scalp EEG with the 10-20 system, intracranial EEG (iEEG) recorded with depth electrodes, waveforms and spike times of neurons recorded in the medial temporal lobe, and localization information on the depth electrodes. Subject characteristics and information on each trial (set size, match/mismatch, correct/incorrect, response, and response time) are given. For technical validation of data, we provide spike sorting quality metrics and the spectra of iEEG and scalp EEG signals. This dataset enables the investigation of working memory by providing simultaneous scalp EEG and iEEG recordings, which can be used for connectivity analysis, along with hard-to-obtain neuronal recordings from humans.


Assuntos
Eletrocorticografia , Memória de Curto Prazo , Neurônios/citologia , Couro Cabeludo , Lobo Temporal/citologia , Epilepsia , Humanos , Convulsões/diagnóstico
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