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1.
Plant Physiol Biochem ; 167: 1035-1048, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34600181

RESUMO

Lonicera japonica Thunb. is widely used in traditional medicine systems of East Asian and attracts a large amount of studies on the biosynthesis of its active components. Currently, there is little understanding regarding the regulatory mechanisms behind the accumulation of secondary metabolites during its developmental stages. In this study, published transcriptomic and proteomic data were mined to evaluate potential linkage between protein modification and secondary metabolism during the floral development. Stronger correlations were observed between differentially expressed genes (DEGs) and their corresponding differentially abundant proteins (DAPs) in the comparison of juvenile bud stage (JBS)/third green stage (TGS) vs. silver flowering stage (SFS). Seventy-five and 76 cor-rDEGs and cor-rDAPs (CDDs) showed opposite trends at both transcriptional and translational levels when comparing their levels at JBS and TGS relative to those at SFS. CDDs were mainly involved in elements belonging to the protein metabolism and the TCA cycle. Protein-protein interaction analysis indicated that the interacting proteins in the major cluster were primarily involved in TCA cycle and protein metabolism. In the simple phenylpropanoids biosynthetic pathway of SFS, both phospho-2-dehydro-3-deoxyheptonate aldolase (PDA) and glutamate/aspartate-prephenate aminotransferase (AAT) were decreased at the protein level, but increased at the gene level. A confirmatory experiment indicated that protein ubiquitination and succinylation were more prominent during the early floral developmental stages, in correlation with simple phenylpropanoids accumulation. Taken together, those data indicates that phenylpropanoids metabolism and floral development are putatively regulated through the ubiquitination and succinylation modifications of PDA, AAT, and TCA cycle proteins in L. japonica.


Assuntos
Lonicera , Flores , Processamento de Proteína Pós-Traducional , Proteômica , Metabolismo Secundário
2.
J Plant Res ; 134(6): 1311-1321, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34351552

RESUMO

Honeysuckle (Lonicera japonica Thunb.) is a traditional medicinal plant in China which is often threatened by high temperature at midday during summer. Heat-induced effects on the photosynthetic apparatus in honeysuckle are associated with a depression of the photosystem II (PSII) photochemical efficiency. However, very limited information is available on regulation of photosynthetic electron flow in PSI photoprotection in heat-stressed honeysuckle. Simultaneous analyses of chlorophyll fluorescence and the change in absorbance of P700 showed that energy transformation and electron transfer activity in PSII decreased under heat stress, but the fraction of photo-oxidizable PSI (Pm) remained stable. With treatments at 38 and 42 °C, the photochemical electron transport in PSII was suppressed, whereas the cyclic electron flow (CEF) around PSI was induced. In addition, the levels of high energy state quenching (qE) and P700 oxidation increased significantly with increasing temperature. However, a decline of qE in antimycin A (AA)- or 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU)-treated leaves after heat treatment was observed, while P700 oxidation decreased only in the presence of AA. The results indicate that heat-induced inhibition of PSII and induction of CEF cooperatively protect PSI from ROS damages through moderate down-regulation of photosynthetic electron flow from PSII to PSI.


Assuntos
Lonicera , Complexo de Proteína do Fotossistema I , Clorofila , Regulação para Baixo , Transporte de Elétrons , Luz , Lonicera/metabolismo , Fotossíntese , Complexo de Proteína do Fotossistema I/metabolismo , Complexo de Proteína do Fotossistema II/metabolismo , Folhas de Planta/metabolismo
3.
J Med Entomol ; 58(6): 2358-2367, 2021 11 09.
Artigo em Inglês | MEDLINE | ID: mdl-34397096

RESUMO

As tick-borne disease incidence increases and pathogens expand into new areas, the need for effective tick management strategies is paramount. In this 5-yr study (2014-2018) conducted in south central Wisconsin, we assessed whether an integrated tick management approach, deployed during peak tick activity (May-August), was more effective at reducing black-legged ticks (Ixodes scapularis Say (Ixodida: Ixodidae)), than individual interventions. Using a factorial design, invasive vegetation removal (Amur honeysuckle, Lonicera maackii Ruprecht (Dipsacales: Caprifoliaceae) and common buckthorn, Rhamnus cathartica Linnaeus (Rosales: Rhamnaceae)) was coupled with deployments of permethrin-treated cotton nesting materials (tick tubes) that target the white-footed mouse (Peromyscus leucopus Rafinesque (Rodentia: Cricetidae)). Results show that the probability of encountering a larval tick by drag sampling was unaffected by treatments at the cumulative 5-yr level. However, vegetation removal significantly reduced larval encounters in 2014, 2015, and 2018, by 33%, 57%, and 61% respectively, and reduced the density of questing nymphal (DON) ticks by 45% in 2015 compared to controls. Despite the limited effect on DON, vegetation removal significantly reduced the cumulative 5-yr density of Borrelia burgdorferi sensu stricto infected nymphs (DIN) (70%) compared to controls as a result of decreased nymphal infection prevalence. Sites treated with tick tubes had lower DIN (66%) and DON (54%) across the study and nymphs were reduced every year following the initial year of deployment compared to controls. Combining treatments did not further reduce DIN or DONs. We conclude that long-term integration of tick tubes with invasive vegetation removal does not provide additional benefit over individual treatments alone.


Assuntos
Acaricidas , Ixodes , Lonicera , Permetrina , Plantas , Rhamnus , Controle de Ácaros e Carrapatos , Animais , Ixodes/crescimento & desenvolvimento , Ninfa/crescimento & desenvolvimento , Densidade Demográfica , Controle de Ácaros e Carrapatos/instrumentação , Wisconsin
4.
J Agric Food Chem ; 69(28): 7938-7947, 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34237214

RESUMO

A novel cell-free biosynthesis system based on a mixture of chassis cell extracts and purified Spy-cyclized enzymes (CFBS-mixture) was developed. As a demonstration, the CFBS-mixture was applied to chlorogenic acid (CGA) biosynthesis. The mix-and-match and Plackett-Burman experiments demonstrated that Lonicera japonica hydroxycinnamate-CoA quinate transferase and p-hydroxyphenylacetate 3-hydroxylase were the key enzymes for the production of CGA. After optimization of the concentrations of the biosynthetic enzymes in the CFBS-mixture reaction using the Plackett-Burman experimental design and the path of the steepest ascent, 711.26 ± 15.63 mg/L CGA was produced after 16 h, which is 71.1-fold the yield obtained using the conventional crude extract-based CFBS and 9.1-fold the reported yield obtained using the living cells. Based on the CFBS-mixture results, the production of CGA was further enhanced in engineered Escherichia coli. The CFBS-mixture strategy is highly effective and will be useful for high-level CFBS of natural products.


Assuntos
Ácido Clorogênico , Lonicera , Extratos Celulares , Ácido Quínico
5.
Zhongguo Zhong Yao Za Zhi ; 46(11): 2746-2752, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34296571

RESUMO

As a traditional Chinese medicinal material, Lonicera japonica has a long medicinal history. The chemical constituents of Lonicera japonica are complex, mainly including iridoid glycosides, flavonoids, triterpenes, organic acids and volatile oil. Iridoid glycosides account for a higher proportion. In addition, modern pharmacological studies have shown that the iridoid glycosides have many pharmacological activities such as antivirus, anti-inflammation, anti-tumor, liver protection and lowering blood sugar. This review intends to systematically summarize the iridoid glycosides identified from Lonicera japonica and their pharmacological activities by searc-hing Chinese and English databases, in order to provide a reference for the further development and utilization of Lonicera japonica and for the improvement of quality standards of medicinal materials.


Assuntos
Lonicera , Anti-Inflamatórios , Flavonoides , Glicosídeos/farmacologia , Glicosídeos Iridoides/farmacologia , Extratos Vegetais
6.
Zhongguo Zhong Yao Za Zhi ; 46(11): 2798-2805, 2021 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-34296578

RESUMO

In order to study the regulation mechanism of secondary metabolites biosynthesis in Lonicera macranthoides, the key genes involved in the regulation of biosynthesis and the mechanism of differential metabolites were explored. In this study, high-throughput sequencing technology was used for transcriptome sequencing of L. macranthoides at different development stages. By using Liquid chromatography-tandem mass spectrometry(LC-MS/MS) technology, the laws of qualitative, quantitative and synthetic accumulation of its metabolites were studied, and the key enzyme genes for the biosynthesis of phenolic acid and flavonoids were screened out according to the differentially expressed genes. A total of 111 differentially accumulate metabolites(DAM) and 6 653 differentially expressed genes(DGE) were obtained by metabonomics and transcriptomics analysis. The metabolites and key enzyme genes in the Erqing(KE) were significantly different from those in the Dabai(KD) and Yinhua(KY) stages. In the phenylalanine biosynthesis pathway, the ion abundance of chlorogenic acid, naringin, quercetin, rutin, coniferol and other metabolites decreased with the development of flowers, while the ion abundance of ferulic acid, coumarin and syringoside increased with the development of flowers. Key enzyme genes such as CHS, HCT, CCR, FLS and COMT positively regulate the downstream metabolites, while PAL, C4H and 4CL negatively regulate the downstream metabolites. This study provides candidate genes and theoretical basis for the further exploration of key enzymes in the biosynthesis of secondary metabolites and for the regulation of the accumulation of secondary metabolites in L. macranthoides by molecular biological methods.


Assuntos
Lonicera , Cromatografia Líquida , Flores/genética , Lonicera/genética , Metabolômica , Proteômica , Espectrometria de Massas em Tandem
7.
Plant Sci ; 308: 110924, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34034872

RESUMO

Lonicera macranthoides Hand-Mazz is an important medicinal plant widely distributed in southern China that has long been used in Chinese traditional medicines. Chlorogenic acid (CGA, 3-caffeoylquinic acid) is the major biologically active ingredient in L. macranthoides. Although key CGA biosynthetic genes have been well documented, their transcriptional regulation remains largely unknown. In this study, we observed that a R2R3 MYB transcription factor LmMYB15 showed a significant correlation with CGA content, indicating its potential role in CGA biosynthesis. A yeast two-hybrid assay suggested that LmMYB15 functions as a transcriptional activator. Overexpression of LmMYB15 in tobacco led to increased accumulation of CGA compared to those in wild-type leaves. To elucidate its functional mechanism, genome-wide DAP-seq was employed and identified the conserved binding motifs of LmMYB15, that is [(C/T) (C/T) (C/T) ACCTA(C/A) (C/T) (A/T)], as well as its direct downstream target genes, including 4CL, MYB3, MYB4, KNAT6/7, IAA26, and ETR2. Subsequently, yeast one-hybrid and dual-luciferase reporter assays verified that LmMYB15 could bind and activate the promoters of 4CL, MYB3 and MYB4, thereby facilitating CGA biosynthesis and phenylpropanoid metabolism. Our findings provide a new track for breeding strategies aiming to enhance CGA content in L. macranthoides that can significantly contribute to better mechanical properties.


Assuntos
Ácido Clorogênico/metabolismo , Lonicera/genética , Proteínas de Plantas/genética , Metabolismo Secundário , Fatores de Transcrição/genética , Sequência de Aminoácidos , Sequência de Bases , Lonicera/metabolismo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Alinhamento de Sequência , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo
8.
Food Res Int ; 143: 110281, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33992381

RESUMO

In this study, H2O2-Vc (hydrogen peroxide-ascorbic acid) oxidation system under 50 and 70 °C was developed to degrade blue honeysuckle polysaccharides. The results suggested that viscosity-average molecular weight was decreased and reducing sugar content was raised with the rise of time or temperature. The degradation was fitted to the second-order reaction kinetics. The gas chromatography revealed two degraded polysaccharides were consisted of six monosaccharides (Gal A, Rha, Ala, Gal, Glu, Man) with different ratios, and eight types of sugar residues were confirmed using nuclear magnetic resonance. Based on the linearity (R1), branching (R2), branch size (R3) calculation, PD70 (purified degraded polysaccharide at 70 °C) had a more linear structure and longer side chains comparing to PD50 (purified degraded polysaccharide at 50 °C). The absence of triple helical structure and sheet-like aggregation with rough surface were confirmed by the Congo red test and scanning electron. Rheological characterization proved the two degraded polysaccharides exhibited shear-thinning behavior and viscoelastic property. Besides, the two degraded polysaccharides displayed strong antiglycation activities, inhibitory effects against α-amylase and α-glucosidase, and exhibited competitive inhibitory kinetics. These findings support the potential application of blue honeysuckle polysaccharides as the treatment of diabetes.


Assuntos
Hipoglicemiantes , Lonicera , Polissacarídeos/farmacologia , Peróxido de Hidrogênio , Hipoglicemiantes/farmacologia , Lonicera/química , alfa-Glucosidases
9.
Zhongguo Zhong Yao Za Zhi ; 46(9): 2220-2228, 2021 May.
Artigo em Chinês | MEDLINE | ID: mdl-34047124

RESUMO

A new method of MS/MS~(ALL) was designed to sequentially record a MS~2 spectrum at each unit mass window through gas phase fractionation concept, so as to offer an opportunity for universal MS~2 spectral recording with direct infusion(DI). As a proof-of-concept, DI-MS/MS~(ALL) was applied for rapid chemome profiling of a famous herbal medicine named Lonicerae Japonicae Flos. After each MS~2 spectrum was correlated to its precursor ion, the structural annotation was conducted by applying well-defined mass cracking rules, matching the mass spectral data with literatures and referring to those accessible databases. As a result, a total of 54 components were identified from Lonicerae Japonicae Flos extract, including 21 phenolic acids, 13 flavonoids, 12 iridoids, 4 triterpenoids and 4 other compounds. Therefore, DI-MS/MS~(ALL) is a powerful tool for comprehensive, rapid qualitative analysis of chemical profiles of traditional Chinese medicine and other chemical components of complex systems.


Assuntos
Medicamentos de Ervas Chinesas , Lonicera , Cromatografia Líquida de Alta Pressão , Extratos Vegetais , Espectrometria de Massas em Tandem
10.
PLoS One ; 16(5): e0251390, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34038434

RESUMO

Lonicera macranthoides Hand.-Mazz (L. macranthoides) is a medicinal herb that is widely distributed in South China. The developmental stage and corolla dehiscence of the flower are the important factors affecting the quality of medicinal ingredients. However, neither the regulatory mechanism controlling chlorogenic acids biosynthesis in L. macranthoides nor the molecular basis of effect of corolla dehiscence on the quality of medicinal materials is fully understood. In this study, metabolomics and transcriptomics were used to analyze the metabolic and transcriptional differences of two different cultivars closed bud type (Bt), and flowering type (Ft), as well as the effect of jasmonic acid methyl ester (MeJA) on chlorogenic acids (CGAs) biosynthesis. In total, large number of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were filtered among three lines of samples. Gene metabolite correlation analyses revealed a 'core set' of 30 genes and 54 genes that were strongly correlated with CGAs biosynthesis and regulating the flowering, respectively. Quantitative real-time polymerase chain reaction results proved the alterations in the expression levels of genes encoding the pathways involved in CGAs biosynthesis. The ion abundances of CGAs were most significantly increased, while some of the CGAs derived and Caffeoyl-CoA-derived substances showed the most largely reduced abundances in the closed bud type (Bt) compared to the flowering type (Ft). MeJA may leads to the activation of downstream genes in CGAs biosynthesis pathway. Overall, there were significant differences in the transcriptional and metabolic levels of CGAs biosynthesis pathway in flower buds of different flowering cultivars. The redirection of metabolic flux may contribute to increased accumulation of CGAs. However, whether MeJA and flowering have direct effects on the accumulation of CGAs needs further studied. These researches effectively expanded the functional genomic library and provide new insights into CGAs biosynthesis in L. macranthoides.


Assuntos
Vias Biossintéticas/genética , Ácido Clorogênico/metabolismo , Lonicera/genética , Metaboloma/genética , Plantas Medicinais/genética , Transcriptoma/genética , Flores/genética , Perfilação da Expressão Gênica/métodos , Metabolômica/métodos
11.
Int J Med Sci ; 18(11): 2285-2293, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33967604

RESUMO

This study examined the effect of the Flos Lonicerae Japonicae water extract (FLJWE), chlorogenic acid, and luteolin on pseudorabies virus (PRV)-induced inflammation in RAW264.7 cells and elucidated related molecular mechanisms. The results revealed that FLJWE and luteolin, but not chlorogenic acid, inhibited the production of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and inflammatory cytokines in PRV-infected RAW 264.7 cells. We found that the FLJWE and luteolin suppressed nuclear factor (NF)-κB activation by inhibiting the phosphorylation of signal transducer and activator of transcription 1 and 3 (STAT1 and STAT3, respectively). Moreover, the FLJWE significantly upregulated the expression of pNrf2 and its downstream target gene heme oxygenase-1 (HO-1). Our data indicated that FLJWE and luteolin reduced the expression of proinflammatory mediators and inflammatory cytokines, such as COX-2 and iNOS, through the suppression of the JAK/STAT1/3-dependent NF-κB pathway and the induction of HO-1 expression in PRV-infected RAW264.7 cells. The findings indicate that the FLJWE can be used as a potential antiviral agent.


Assuntos
Anti-Inflamatórios/farmacologia , Antivirais/farmacologia , Lonicera/química , Extratos Vegetais/farmacologia , Viroses/tratamento farmacológico , Animais , Anti-Inflamatórios/isolamento & purificação , Anti-Inflamatórios/uso terapêutico , Antivirais/isolamento & purificação , Antivirais/uso terapêutico , Modelos Animais de Doenças , Flores/química , Heme Oxigenase-1/metabolismo , Herpesvirus Suídeo 1/imunologia , Humanos , Inflamação/tratamento farmacológico , Inflamação/imunologia , Inflamação/virologia , Proteínas de Membrana/metabolismo , Camundongos , NF-kappa B/metabolismo , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/uso terapêutico , Células RAW 264.7 , Fator de Transcrição STAT1/metabolismo , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/imunologia , Viroses/imunologia , Viroses/virologia , Água/química
12.
Anal Chim Acta ; 1161: 338475, 2021 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-33896554

RESUMO

The common elution process of molecularly imprinted polymers (MIPs) is carried out in an acidic medium, which greatly affects the stability and reusability of synthetic MIPs, especially for magnetic MIPs. In this study, we fabricated an acid-resistant imprinted layer formed by phase-transitioned lysozyme on magnetic nanomaterials for selective extraction of chlorogenic acid in Honeysuckle, which often coexists with structural analogs. The newly designed acid-resistant imprinted layer can not only protect the internal magnetic core from denudation and dissolution, but also maintain the integrity of the imprinted layer during the elution process. The resultant magnetic MIPs exhibited good stability with no change on morphology after the repeatedly eluting process, and satisfactory reusability that can be used at least ten adsorption-desorption cycles with almost no decrease for adsorption capacity. In addition, the resultant materials possess satisfactory magnetism, uniform morphology with typical core-shell structure, stable crystallization, and good adsorption performance showing on high adsorption amount (10.82 mg g-1), fast kinetic equilibrium time (as short as 30 min), and satisfactory selectivity (IF = 2.85, SC > 1.5). At last, the obtained magnetic MIPs as adsorbents coupled with HPLC were successfully used to selective extract CGA in Honeysuckle samples with the high recoveries in the range of 92.0-104.4%, and the contents of CGA in Honeysuckle samples from the different origin are calculated in the range of 0.98%-1.24%.


Assuntos
Lonicera , Impressão Molecular , Nanoestruturas , Adsorção , Ácido Clorogênico , Fenômenos Magnéticos , Polímeros , Extração em Fase Sólida
13.
Artigo em Inglês | MEDLINE | ID: mdl-33900906

RESUMO

During an investigation of the yeast communities associated with wild fruit shrubs in Dagestan (Caucasus, Russia), four fermenting ascospore-producing yeast strains were isolated from leaves of the Georgian honeysuckle (Lonicera iberica M. Bieb.) and from soil underneath this plant. Phylogenetic analyses based on concatenated sequences of the ITS region and D1/D2 domains of the large subunit rRNA gene and concatenated sequences of the ribosomal DNA cystron, RPB2 and TEF1 genes showed that the isolated strains represented a new species of the genus Zygotorulaspora. The new species was placed in the basal position to other species of the clade and close to Zygotorulaspora mrakii. Based on the results of phylogenetic analyses and the phenotypic characteristics of the four studied strains, a novel species is described, for which the name Zygotorulaspora dagestanica sp. nov. is proposed. The holotype is KBP Y-4591T, three metabolically inactive cryopreserved isotype cultures are DSM 100088, VKM Y-3060 and VKPM Y-4318. The MycoBank number is MB 838285.


Assuntos
Lonicera/microbiologia , Filogenia , Saccharomycetales/classificação , Microbiologia do Solo , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Folhas de Planta/microbiologia , Federação Russa , Saccharomycetales/isolamento & purificação , Análise de Sequência de DNA
14.
Talanta ; 226: 122134, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33676688

RESUMO

Deep investigation, profiling of chemical diversity of constituent compounds and discovery of novel structures is a great challenge. A novel comprehensive and effective approach to mine trace unknown compounds combined with structure recognition in complex matrix is developed, in order to profiling potential Chlorogenic acids (CGAs) in Lonicera Flos (LFs): using multiple neutral loss/precursor ion (NL/PI) markers scans combined with high resolution mass spectrometry (HRMS). The workflow included (i) Fragmentation rules deduced by Q-orbitrap and selection of multiple NL/PI markers. (ii) Multiple NL/PI marker scans and grouping of peaks that had responses on two or more channels. (iii) Alignment of peaks in Full-MS scan and multiple NL/PI scans. (iv) The precursor ions list was introduced to mine novel CGAs according to simulated molecular formula. (v) Identification and structure recognition with the aid of HRMS. The procedure proved to be valid to screen and identify 51 CGAs from Lonicera Flos (LFs) with 16 categories, especially dihydroxyphenyl and glucoside for the first time. Its application could also be extended for global profiling of other complicated chemical systems, such as Chinese medicinal formulas.


Assuntos
Lonicera , Ácido Clorogênico , Cromatografia Líquida de Alta Pressão , Glucosídeos , Espectrometria de Massas
15.
Viruses ; 13(2)2021 02 16.
Artigo em Inglês | MEDLINE | ID: mdl-33669264

RESUMO

Honeysuckle (Lonicera japonica Thunb) is a traditional Chinese medicine (TCM) with an antipathogenic activity. MicroRNAs (miRNAs) are small non-coding RNA molecules that are ubiquitously expressed in cells. Endogenous miRNA may function as an innate response to block pathogen invasion. The miRNA expression profiles of both mice and humans after the ingestion of honeysuckle were obtained. Fifteen overexpressed miRNAs overlapped and were predicted to be capable of targeting three viruses: dengue virus (DENV), enterovirus 71 (EV71) and SARS-CoV-2. Among them, let-7a was examined to be capable of targeting the EV71 RNA genome by reporter assay and Western blotting. Moreover, honeysuckle-induced let-7a suppression of EV71 RNA and protein expression as well as viral replication were investigated both in vitro and in vivo. We demonstrated that let-7a targeted EV71 at the predicted sequences using luciferase reporter plasmids as well as two infectious replicons (pMP4-y-5 and pTOPO-4643). The suppression of EV71 replication and viral load was demonstrated in two cell lines by luciferase activity, RT-PCR, real-time PCR, Western blotting and plaque assay. Furthermore, EV71-infected suckling mice fed honeysuckle extract or inoculated with let-7a showed decreased clinical scores and a prolonged survival time accompanied with decreased viral RNA, protein expression and virus titer. The ingestion of honeysuckle attenuates EV71 replication and related pathogenesis partially through the upregulation of let-7a expression both in vitro and in vivo. Our previous report and the current findings imply that both honeysuckle and upregulated let-7a can execute a suppressive function against the replication of DENV and EV71. Taken together, this evidence indicates that honeysuckle can induce the expression of let-7a and that this miRNA as well as 11 other miRNAs have great potential to prevent and suppress EV71 replication.


Assuntos
Antivirais/farmacologia , Enterovirus Humano A/efeitos dos fármacos , Lonicera/química , MicroRNAs/metabolismo , Extratos Vegetais/farmacologia , Replicação Viral/efeitos dos fármacos , Animais , Linhagem Celular , Enterovirus Humano A/fisiologia , Infecções por Enterovirus/tratamento farmacológico , Humanos , Camundongos , Camundongos Endogâmicos ICR
16.
J Genet ; 1002021.
Artigo em Inglês | MEDLINE | ID: mdl-33764335

RESUMO

AGLl9 is an important regulator for flowering in plants and critical in controlling the morphogenesis of flower organs. The fulllength cDNAs of AGL19in conventional Lonicera macranthoides (Lm-AGL19) and the mutant 'Xianglei' cultivar (Lm-XL-AGL19) were obtained using rapid amplification of cDNA ends and the expression vectors for Lm-XL-AGL19were constructed to investigate the roles of AGL19 in the 'Xianglei' cultivar. Lm-AGL19 (GenBank: MK419948) and Lm-XL-AGL19 (GenBank: MK419948) were isolated from the conventional variety and 'Xianglei' cultivar of L. macranthoides, respectively. Lm-AGL19 is 1274 bp in length, whereas Lm-XL-AGL19 is 1264-bp long, and both include a 654 bp open reading frame, encoding 217 amino acids, which has a highly conserved MADS_MEF2_like domain and a moderately conserved K-box domain, belonging to the type II MADS-box family of genes. Quantitative real-time polymerase chain reaction indicated that the expression levels of these genes at different flowering stages were significantly different, and that the genes were also expressed in stems and leaves. Lm-XL-AGL19 is underexpressed at flowering period 5 that the key time node for corolla expansion and nonexpansion, while LM-AGL19 is overexpressed during this flowering period. AGL19 was speculated to be a functional gene causing different phenotypes in the two L. macranthoides varieties. The successfully constructed plant expression vector provides an experimental reference for further research on the function of this gene and the basis for the excellent phenotype of L. macranthoides 'Xianglei'.


Assuntos
Flores/metabolismo , Regulação da Expressão Gênica de Plantas , Lonicera/metabolismo , Proteínas de Domínio MADS/metabolismo , Proteínas de Plantas/metabolismo , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Flores/classificação , Flores/genética , Flores/crescimento & desenvolvimento , Lonicera/classificação , Lonicera/genética , Lonicera/crescimento & desenvolvimento , Proteínas de Domínio MADS/genética , Filogenia , Proteínas de Plantas/genética , Homologia de Sequência
17.
World J Microbiol Biotechnol ; 37(3): 44, 2021 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-33547493

RESUMO

In this study, we investigated effects of lead on growth response and antioxidant defense protection in a new identified strain isolated from a soil, in the rhizosphere of Sainfoin Hedysarum coronarium L. Different concentrations of lead (0, 0.2, 1.5 and 3 g L-1) added to Bacillus simplex strain 115 cultures surprisingly did not inhibit its growth. However, a resulting oxidative stress as attested by overproduction of H2O2 (+ 6.2 fold) and malondialdehyde (+ 2.3 fold) concomitantly to the enhancement of proteins carbonylation (+ 221%) and lipoxygenase activity (+ 59%) was observed in presence of 3 g L-1 of lead. Intrinsic antioxidant defenses were revealed by the coupled up-regulation of catalase (+ 416%) and superoxide dismutase (+ 4 fold) activities, with a more important Fe-SOD increase in comparison to the other isoforms. Bioaccumulation assays showed both intracellular and extracellular lead accumulation. Biosorption was confirmed as a particularly lead resistance mechanism for Bacillus simplex strain 115 as the metal sequestration in cell wall accounted for 88.5% to 98.5% of the total endogenous metal accumulation. Potentiality of this new isolated microorganism as a biotechnological tool for agricultural soil lead bioremediation was thus proposed.


Assuntos
Bacillus/crescimento & desenvolvimento , Chumbo/efeitos adversos , Lonicera/microbiologia , Bacillus/efeitos dos fármacos , Bacillus/metabolismo , Biodegradação Ambiental , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Peróxido de Hidrogênio/metabolismo , Lipoxigenase/metabolismo , Malondialdeído/metabolismo , Viabilidade Microbiana/efeitos dos fármacos , Rizosfera , Microbiologia do Solo , Superóxido Dismutase/metabolismo
18.
BMC Plant Biol ; 21(1): 98, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33596836

RESUMO

BACKGROUND: Plants have remarkable diversity in petal colour through the biosynthesis and accumulation of various pigments. To better understand the mechanisms regulating petal pigmentation in Lonicera japonica, we used multiple approaches to investigate the changes in carotenoids, anthocyanins, endogenous hormones and gene expression dynamics during petal colour transitions, i.e., green bud petals (GB_Pe), white flower petals (WF_Pe) and yellow flower petals (YF_Pe). RESULTS: Metabolome analysis showed that YF_Pe contained a much higher content of carotenoids than GB_Pe and WF_Pe, with α-carotene, zeaxanthin, violaxanthin and γ-carotene identified as the major carotenoid compounds in YF_Pe. Comparative transcriptome analysis revealed that the key differentially expressed genes (DEGs) involved in carotenoid biosynthesis, such as phytoene synthase, phytoene desaturase and ζ-carotene desaturase, were significantly upregulated in YF_Pe. The results indicated that upregulated carotenoid concentrations and carotenoid biosynthesis-related genes predominantly promote colour transition. Meanwhile, two anthocyanins (pelargonidin and cyanidin) were significantly increased in YF_Pe, and the expression level of an anthocyanidin synthase gene was significantly upregulated, suggesting that anthocyanins may contribute to vivid yellow colour in YF_Pe. Furthermore, analyses of changes in indoleacetic acid, zeatin riboside, gibberellic acid, brassinosteroid (BR), methyl jasmonate and abscisic acid (ABA) levels indicated that colour transitions are regulated by endogenous hormones. The DEGs involved in the auxin, cytokinin, gibberellin, BR, jasmonic acid and ABA signalling pathways were enriched and associated with petal colour transitions. CONCLUSION: Our results provide global insight into the pigment accumulation and the regulatory mechanisms underlying petal colour transitions during the flower development process in L. japonica.


Assuntos
Flores/metabolismo , Lonicera/genética , Pigmentos Biológicos/biossíntese , Cor , Flores/genética , Flores/crescimento & desenvolvimento , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Geranil-Geranildifosfato Geranil-Geraniltransferase/genética , Geranil-Geranildifosfato Geranil-Geraniltransferase/metabolismo , Lonicera/crescimento & desenvolvimento , Lonicera/metabolismo , Oxirredutases/genética , Oxirredutases/metabolismo , Oxigenases/genética , Oxigenases/metabolismo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Transcriptoma
19.
Biomed Res Int ; 2021: 8878633, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33490281

RESUMO

Lonicera japonica Thunb is a traditional Chinese herbal medicine for treating intestinal inflammation. The extraction method of Lonicera japonica Thunb polysaccharide (LJP) has been developed previously by our research group. In this study, a Fourier transform infrared spectrometer (FT-IR) was used to perform a qualitative analysis of LJP and a precolumn derivatization high-performance liquid chromatography (HPLC) ((Palo Alto, CA, USA) method was used to explore the monosaccharide composition of LJP. Then, we studied the effect of LJP on the intestinal flora and immune functions of dextran sulfate sodium- (DSS-) induced colitis ulcerative mouse models. The results showed that LJP was consisted of 6 types of monosaccharides and had the characteristic absorption of typical polysaccharides. LJP can increase significantly the weight, organ index, serum cytokines (interleukin, tumor necrosis factor, and interferon-γ), secretory immunoglobulin A (SIgA) concentration, and natural killer (NK) cell and cytotoxic lymphocyte (CTL) activities in DSS-treated mice. The results of intestinal flora showed that a high dose (150 mg kg-1) of LJP had the best effects on improving the intestinal probiotics (Bifidobacterium and Lactobacilli) and antagonizing the pathogenic bacteria (Escherichia coli and Enterococcus). In addition, the measurement results of the spleen lymphocyte apoptosis confirmed from another perspective that LJP had protective effects of immune cells for DSS-treated mice.


Assuntos
Colite Ulcerativa/metabolismo , Lonicera/química , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Substâncias Protetoras/farmacologia , Animais , Colite Ulcerativa/induzido quimicamente , Sulfato de Dextrana/efeitos adversos , Modelos Animais de Doenças , Microbioma Gastrointestinal/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos BALB C
20.
Chin J Nat Med ; 19(1): 70-80, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33516454

RESUMO

Pesticides' overuse and misuse have been reported to induce ingredient variations in herbal medicine, which is now gaining attention in the medicinal field as a form of alternative medicine. To date, available studies on pesticide-induced ingredient variations of herbal medicine are limited only on a few compounds and remain most others unexamined. In this study, a plant metabolomics-based strategy was performed to systematically explore the effects of two frequently used insecticides on the comprehensive constituents of Lonicerae Japonicae Flos (LJF), the flower buds of Lonicera japonica Thunb. Field trials were designed on a cultivating plot of L. japonica with controls and treatments of imidacloprid (IMI) and compound flonicamid and acetamiprid (CFA). Unbiased metabolite profiling was conducted by ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometer. After data pretreatment by automatic extraction and screening, a data matrix of metabolite features was submitted for statistical analyses. Consequently, 29 metabolic markers, including chlorogenic acids, iridoids and organic acid-glucosides were obtained and characterized. The relative quantitative assay was subsequently performed to monitor their variations across flowering developments. This is the first study that systematically explored the insecticide-induced metabolite variations of LJF while taking into account the inherent variability of flowering development. The results were beneficial for holistic quality assessment of LJF and significant for guiding scientific use of pesticides in the large-scale cultivation.


Assuntos
Medicamentos de Ervas Chinesas , Inseticidas , Lonicera , Metabolômica , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/química , Flores/metabolismo , Inseticidas/farmacologia , Lonicera/efeitos dos fármacos , Lonicera/metabolismo , Plantas Medicinais/efeitos dos fármacos , Plantas Medicinais/metabolismo
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