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1.
Carbohydr Polym ; 304: 120503, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36641169

RESUMO

Rheumatoid arthritis (RA) is a chronic inflammatory immune and lubrication dysfunction disease that causes great damage to the joints. Herein, inspired by the unique biochemistry structure and excellent hydration of chondroitin sulfate (CHI) existing in joint system, one kind of novel polysaccharide nanoparticle lubricant, that is chitosan nanoparticles (CS NPs) grafting CHI (CS-CHI), is synthesized by one-step surface chemistry reaction. CHI with negative charges can form hydration layers on the surface of CS NPs, thus improving the lubricity of nanoparticles. Simultaneously, CS-CHI NPs have effective loading and sustained drug release ability for anti-inflammatory drug diclofenac sodium (DS), along with good biocompatibility. Finally, based on a collagen-induced rat RA model, in vitro animals experimental results indicate that the as-synthesized CS-CHI@DS NPs has obvious inhibitory effects on inflammatory factors and can effectively prevent the damaged cartilage from further destruction.


Assuntos
Quitosana , Nanopartículas , Ratos , Animais , Quitosana/química , Água/química , Lubrificantes , Biomimética , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Diclofenaco/farmacologia , Diclofenaco/uso terapêutico , Nanopartículas/química , Portadores de Fármacos/química
2.
Int J Biol Macromol ; 227: 673-684, 2023 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-36529226

RESUMO

The aim of this work was to study the influence of the acetylation process of kraft lignin on developing dispersions potentially applicable as new bio-based semisolid lubricants. Lignin was functionalized with acetic anhydride and pyridine as a catalyst by modifying different reaction variables (temperature, ratio of pyridine/acetic anhydride and time). Acetylated lignin was analyzed using FTIR, 1H and 13C NMR techniques, TGA, DSC and SEM to evaluate the chemical, morphological and thermal changes induced by the acetylation process. The influence of the acetylation process on the rheological and tribological properties of dispersions was related to the development of different microstructures, which depend on chemical and morphological properties of acetylated lignin. In this sense, two different rheological behaviours (gel-like or fluid-like) were found to depend on the reaction time. From the experimental results obtained, it can be concluded that the acetylation process is a key issue to modulate rheological and morphological properties of dispersions, resulting in an effective method to improve the compatibility of lignin and castor oil. Acetylated lignin with medium degrees of substitution with adequate morphological properties can be potentially used as an effective thickening agent to develop semisolid lubricants.


Assuntos
Lignina , Lubrificantes , Lignina/química , Anidridos Acéticos , Acetilação , Lubrificantes/química
3.
Proc Natl Acad Sci U S A ; 120(1): e2214143120, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36574684

RESUMO

Due to its multifaceted impact in various applications, icing and ice dendrite growth has been the focus of numerous studies in the past. Dendrites on wetting (hydrophilic) and nonwetting (hydrophobic) surfaces are sharp, pointy, branching, and hairy. Here, we show a unique dendrite morphology on state-of-the-art micro/nanostructured oil-impregnated surfaces, which are commonly referred to as slippery liquid-infused porous surfaces or liquid-infused surfaces. Unlike the dendrites on traditional textured hydrophilic and hydrophobic surfaces, the dendrites on oil-impregnated surfaces are thick and lumpy without pattern. Our experiments show that the unique ice dendrite morphology on lubricant-infused surfaces is due to oil wicking into the porous dendritic network because of the capillary pressure imbalance between the surface texture and the dendrites. We characterized the shape complexity of the ice dendrites using fractal analysis. Experiments show that ice dendrites on textured oil-impregnated surfaces have lower fractal dimensions than those on traditional lotus leaf-inspired air-filled porous structures. Furthermore, we developed a regime map that can be used as a design guideline for micro/nanostructured oil-impregnated surfaces by capturing the complex effects of oil chemistry, oil viscosity, and wetting ridge volume on dendrite growth and morphology. The insights gained from this work inform strategies to reduce lubricant depletion, a major bottleneck for the transition of micro/nanostructured oil-impregnated surfaces from bench-top laboratory prototypes to industrial use. This work will assist the development of next-generation depletion-resistant lubricant-infused ice-repellent surfaces.


Assuntos
Excipientes , Gelo , Alimentos , Lubrificantes , Dendritos
4.
Sci Rep ; 12(1): 18662, 2022 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-36333428

RESUMO

In lubricated tribo-contacts, hydrogen ingress in steel is possible due to chemical reactions of lubricant components like base oils or additives, and/or contamination upon service particularly water, and/or corrosion processes, and/or electrostatic fields or current flow. Absorbed by the metal, atomic hydrogen may cause serious deleterious effects on the physical-chemical and mechanical properties, reducing the material's ability to withstand the design loads. The present research work is focused on analyzing the influence of electric field on lubricating oils in contact with steel surface. In order to evaluate the possibility of atomic hydrogen generation and permeation into the steel under cathodic polarization of lubricating oils the electrochemical permeation technique developed by Devanathan and Stachurski is used. The input cell of a Devanathan-Stachurski set up is appropriately modified by realizing a very close distance between the working electrode (steel membrane) and a Pt counter electrode with the oil between. This significantly increases the sensibility of the set up and allows the application of larger voltage and higher temperature to enable hydrogen generation from lubricating oils. The complex effects of cathodic polarization, temperature, additives and presence of water in model lubricating oils on atomic hydrogen permeation into steel is discussed.


Assuntos
Hidrogênio , Aço , Aço/química , Óleos , Eletrodos , Lubrificantes , Água
5.
BMC Res Notes ; 15(1): 342, 2022 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-36335388

RESUMO

OBJECTIVE: As low-dose atropine eye-drops for myopia progression control prepared in-house by diluting the commercial 0.1% atropine eye-drop with sterile water or normal saline has been a common practice whereas atropine injection is readily available and could be a more feasible alternative, this study aimed to assess the properties of the in-house low-dose atropine eye-drops prepared by diluting the atropine injection in two solvents and tested in two temperature conditions. RESULTS: The 0.01% atropine eye-drops contains no bacteria, fungi, or particulate matter. The levels of atropine sulfate on all samples were comparable to the freshly prepared samples at the 12th week, regardless of the solvents used or storage conditions. The low-dose atropine eye-drops prepared from readily available atropine sulfate injection at healthcare facilities could be an alternative to commercial products.


Assuntos
Atropina , Miopia , Humanos , Soluções Oftálmicas , Solução Salina , Midriáticos , Lubrificantes , Hospitais , Solventes
6.
J Obstet Gynaecol Res ; 48(11): 2807-2820, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36319196

RESUMO

AIM: To assess the effect of lubricants on reducing perineal trauma during vaginal delivery. METHODS: PubMed, Embase, the Cochrane Library, CINAHL, China National Knowledge Infrastructure, China Biology Medicine disc, WanFang databases, and ClinicalTrials.gov, were searched for literature up to 25 June 2021. Randomized controlled trials published in English or Chinese that compared the vaginal application of lubricant with standard care for women were included. Two reviewers independently performed study screening, data extraction, risk of bias assessment, and certainty of evidence assessment. Pooled effect sizes and corresponding 95% confidence intervals (CI) were calculated using meta-analysis. RESULTS: Nineteen trials enrolling 5445 pregnant women were included. Compared with standard care, women using lubricants had a lower incidence of perineal trauma (risk ratio [RR] 0.84, 95% CI 0.76-0.93; low certainty evidence), second-degree perineal laceration (RR 0.72, 95% CI 0.64-0.82; moderate certainty evidence) and episiotomy (RR 0.77, 95% CI 0.62-0.96; very low certainty evidence), and had a shorter duration of the second-stage labor (MD -13.72 min, 95% CI -22.68 to -4.77; very low certainty evidence). CONCLUSION: Lubricants might reduce the incidence of perineal trauma, especially second-degree perineal laceration, and shorten the duration of the second-stage labor. More well-designed studies will continue developing high-quality evidence in this field.


Assuntos
Lacerações , Feminino , Gravidez , Humanos , Lacerações/prevenção & controle , Períneo/lesões , Lubrificantes , Ensaios Clínicos Controlados Aleatórios como Assunto , Episiotomia
7.
Chemosphere ; 309(Pt 1): 136622, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36181837

RESUMO

Lubricants operate as antifriction media, preserving machine reliability, facilitating smooth operation, and reducing the likelihood of frequent breakdowns. The petroleum-based reserves are decreasing globally, leading to price increases and raising concerns about environmental degradation. The researchers are concentrating their efforts on developing and commercializing an environmentally friendly lubricant produced from renewable resources. Biolubricants derived from nonedible vegetable oils are environmentally favorable because of their non-toxicity, biodegradability, and close to net zero greenhouse gas emissions. The demand for bio lubricants in industry and other sectors is increasing due to their non-toxic, renewable, and environmentally friendly nature. Good lubrication, anti-corrosion, and high flammability are characteristic properties of vegetable oils due to their unique structure. This study presents several key properties of nonedible oils that are used to produce lubricants via the transesterification process. Bibliometric analysis is also performed, which provides us with a better understanding of previous studies related to the production of bio lubricants from the transesterification process. Only 371 published documents in the Scopus database were found to relate to the production of bio lubricants using the transesterification process. The published work was mostly dominated by research articles (286; 77.088%). Significant development can be seen in recent years, with the highest occurrence in 2021, reaching 68 publications accounting for 18.38% of the total documents. In the second step, (i) the authors with the most number of publications; (ii) journals with the most productions; (iii) most productive countries; and (iv) the authors' most frequently used keywords were evaluated. These results will provide a pathway for researchers interested in this field. Lastly, recommendation is made on research gaps to device possible strategies for its commercialization.


Assuntos
Gases de Efeito Estufa , Petróleo , Reprodutibilidade dos Testes , Lubrificantes/química , Óleos Vegetais
8.
Langmuir ; 38(42): 12944-12950, 2022 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-36240007

RESUMO

Lubricant performance can be improved using additives such as organic friction modifiers (OFMs) and is influenced by their conformation and properties in the space confined between the substrate surfaces, rendering the detailed property analysis of confined OFMs and lubricants a matter of high practical significance. To date, studies on fatty acids as confined OFMs have mainly focused on linear- and unsaturated-chain molecules, leaving branched-chain structures underexplored. To bridge this gap, we used resonance shear measurements in this study to probe the viscosity of two branched-chain C18 fatty acids (isostearic acid T and isostearic acid) confined between mica surfaces at different applied normal loads (L) and surface separation distances (D). The viscosity parameter (bs) of both acids significantly increased at D < ∼4 nm because of structuring and was lower for isostearic acid than that for isostearic acid T at L > ∼0.6 mN. This reversal of bulk viscosity order under nanoconfinement was ascribed to the ability of the bulky methyl-substituted side chain of isostearic acid to prevent ordering in the nanospace between the mica surfaces and thus preserve fluidlike properties. The obtained results provide fundamental insights into the lubricity of branched-chain fatty acids and are expected to promote the development of novel high-performance OFMs.


Assuntos
Silicatos de Alumínio , Ácidos Graxos , Viscosidade , Lubrificantes
9.
J Pak Med Assoc ; 72(9): 1694-1698, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36280958

RESUMO

Objectives: To assess the safety of dermatological 0.1% tacrolimus ointment when used topically and its efficacy in the treatment of vernal keratoconvinctivtis. METHODS: The quasi-experimental, multi-centre study was conducted at the Gujranwala Medical College/District Headquarters Teaching Hospital, Gujranwala, and the Gomal Medial College/Mufti Mehmood Teaching Hospital, Dera Ismail Khan, Pakistan, from July 2019 to March 2020, and comprised patients of severe vernal keratoconvinctivtis. Symptoms and clinical signs were graded on a pre-devised scale. Patients were given small amount of tacrolimus 0.1% ointment applied to the inferior conjunctival fornix before going to bed. The duration of treatment was 3 months and the patients were followed up for up to 6 months. Data was analysed using SPSS 20. RESULTS: Of the 50 patients, 30(60%) were males and 20(40%) were females. The overall mean age was 10.64±3.199 years. Mean symptom score and clinical signs score gradually reduced on each follow-up (p<0.05). Mild recurrence was noted in 12(24%) patients who were managed with lubricants and anti-histamine topical drops. No complication was noted. CONCLUSIONS: Tacrolimus 0.1% was found to be effective and safe in the treatment of severe refractory vernal keratoconvinctivtis even when given once a day. Clinical Trial Registration: Chinese Clinical Trial Registry Id: ChiCTR2000031929 link: www.chictr.org.cn/hvshowproject.aspx?id=28053.


Assuntos
Conjuntivite Alérgica , Tacrolimo , Masculino , Feminino , Humanos , Criança , Adolescente , Tacrolimo/efeitos adversos , Conjuntivite Alérgica/tratamento farmacológico , Conjuntivite Alérgica/induzido quimicamente , Conjuntivite Alérgica/diagnóstico , Pomadas/uso terapêutico , Imunossupressores/efeitos adversos , Resultado do Tratamento , Lubrificantes/uso terapêutico
10.
BMC Musculoskelet Disord ; 23(1): 927, 2022 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-36266652

RESUMO

BACKGROUND: Cartilage is a mechanically highly stressed tissue in the human body and an important part of synovial joints. The joint cartilage is lubricated by synovial fluid with hyaluronic acid (HA) as main component. However, in joints with osteoarthritis HA has a lower concentration and molecular weight compared to healthy joints. In recent years, the intra-articular injection of therapeutic HA lubricant, has become a popular therapy. The effect of HA application on the friction of a complete joint with physiological movement needs to be further determined. METHODS: The aim of the present study was to evaluate the lubrication effect of the joint by three lubricants (NaCl, fetal calf serum (FCS) and HA) and their effect on the friction in nine complete ovine carpo-metacarpal joints. The joints were mounted on a material testing machine and a physiological movement with 10° rotation was simulated with ascending axial load (100 - 400 N). Specimens were tested native, with cartilage damage caused by drying out and relubricated. Dissipated energy (DE) as a measure of friction was recorded and compared. RESULTS: Investigating the effect of axial load, we found significant differences in DE between all axial load steps (p < .001), however, only for the defect cartilage. Furthermore, we could document an increase in DE from native (Mean: 15.0 mJ/cycle, SD: 8.98) to cartilage damage (M: 74.4 mJ/cycle, SD: 79.02) and a decrease after relubrication to 23.6 mJ/cycle (SD: 18.47). Finally, we compared the DE values for NaCl, FCS and HA. The highest values were detected for NaCl (MNorm = 16.4 mJ/cycle, SD: 19.14). HA achieved the lowest value (MNorm = 4.3 mJ/cycle, SD: 4.31), although the gap to FCS (MNorm = 5.1 mJ/cycle, SD: 7.07) was small. CONCLUSIONS: We were able to elucidate three effects in joints with cartilage damage. First, the friction in damaged joints increases significantly compared to native joints. Second, especially in damaged joints, the friction increases significantly more with increased axial load compared to native or relubricated joints. Third, lubricants can achieve an enormous decrease in friction. Comparing different lubricants, our results indicate the highest decrease in friction for HA.


Assuntos
Cartilagem Articular , Ácido Hialurônico , Animais , Fricção , Articulações , Lubrificantes , Soroalbumina Bovina , Ovinos , Cloreto de Sódio , Líquido Sinovial
11.
Int J Pharm ; 628: 122300, 2022 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-36272512

RESUMO

In rotary tablet presses, the powder flow into the dies is typically facilitated by paddle feeder. For internally lubricated formulations, the shear forces exerted by the paddle rotation can result in a considerable decrease in tablet strength due to the dispersion of lubricant agglomerates. Available models to describe the lubricant dispersion in paddle feeder allow only a limited quantitative description and transferability of the process. This study introduces an empirical dispersion kinetic which is able to precisely describe the reduction of compactibility due to the shear stresses inside the paddle feeder, even for inhomogeneously flowing material. Additionally, by blending different grades of magnesium stearate at three levels of lubricant concentration with two different grades of microcrystalline cellulose, the impact of bulk properties on the lubrication dispersion in the feed frame was investigated. It was shown, that for a given formulation, the kinetics of compactibility reduction are comparable for different magnesium stearate concentrations. Additionally, the bulk properties of the applied magnesium stearate grade critically affect the dispersion kinetics as well as the maximum compactibility reduction inside the feed frame. In summary, the developed model represents a meaningful extension of the currently available process models for pharmaceutical tablet lubrication.


Assuntos
Excipientes , Lubrificantes , Lubrificantes/química , Excipientes/química , Comprimidos/química , Ácidos Esteáricos/química , Lubrificação , Pós
12.
Kathmandu Univ Med J (KUMJ) ; 20(77): 43-46, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36273289

RESUMO

Background The advancement in drug development and availability of newer drugs has improved overall health services including decrease in mortality and hospital stay. Along, it has brought negative impacts such as polypharmacy and associated adverse drug reactions and antimicrobial resistance. Drug utilization research is an essential approach to understand the drug use pattern, identify the early signs of such irrational drug use and to improve quality of drug use. Objective To study the drug utilization pattern in the Ophthalmology Outpatient department (OPD) of Dhulikhel Hospital, Kathmandu University Hospital (DH-KUH). Method A descriptive, cross sectional study was conducted from March 2019 to August 2019 in patients attending OPD of Ophthalmology in Dhulikhel Hospital, Kathmandu University Hospital. Prescriptions of 311 patients were analyzed using World Health Organization (WHO) International Network of Rational Use of Drug (INRUD) and additional other indices. The descriptive data was presented in mean and standard deviation. Result The average number of drugs per prescription was 2.10±1.35. Out of total 311 prescriptions, drugs prescribed in generic name were 152 (23.30%). Total antibiotics encountered were 247 (37.90%) and total drugs prescribed from National Essential Medicine List (NEML) were 371 (56.90%). Antibiotics 247 (37.90%) were the most commonly prescribed drugs followed by lubricants 146 (22.40%). Conclusion Practice of polypharmacy was very high. Most of the drugs were prescribed in brand names and antibiotics were the most frequently used drugs.


Assuntos
Pacientes Ambulatoriais , Padrões de Prática Médica , Humanos , Centros de Atenção Terciária , Estudos Transversais , Uso de Medicamentos , Antibacterianos/uso terapêutico , Lubrificantes , Prescrições de Medicamentos
13.
Antimicrob Agents Chemother ; 66(11): e0081622, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36255254

RESUMO

Data to inform behaviorally congruent delivery of rectal microbicides as lubricants are scant. Dapivirine (DPV) is a nonnucleoside reverse transcriptase inhibitor which has been demonstrated to be well-tolerated and efficacious in multiple clinical trials when used in a vaginal ring formulation. DPV gel administered rectally with an applicator was found to be well-tolerated in a phase 1 clinical trial. MTN-033, a single site, open label, sequence randomized, crossover study, enrolled HIV-negative men to receive 0.05% DPV gel intrarectally using an applicator (2.5 g) and self-administered on an artificial phallus as lubricant (up to 10 g). The study evaluated the pharmacokinetics (in plasma, rectal fluid, and mucosal rectal tissue), safety, acceptability, and pharmacodynamics of DPV gel when applied rectally. Statistical comparisons between methods of application were performed using mixed effects models or Wilcoxon's signed rank tests. Sixteen participants used DPV gel by applicator and 15/16 participants used gel as lubricant (mean, 1.8 g; SD, 0.8). DPV plasma AUC0-24h after use as lubricant was estimated to be 0.41 times the AUC0-24h (95% CI 0.24, 0.88) after use with applicator. While DPV was quantifiable in plasma and luminal fluid, it was not quantifiable in tissue for both applicator and as lubricant administration. No related adverse events (AE) were reported, and 15/15 participants felt the gel was easy to use. Evidence of local delivery and systemic absorption of DPV when dosed as an anal lubricant supports the feasibility and potential for development of lubricant-delivered rectal microbicides. There were no safety concerns associated with use of DPV gel and participants reported finding it easy to use. However, lower DPV exposure in plasma and lack of quantifiable DPV in rectal tissue indicate that higher potency, concentration, and longer half-life antiretrovirals with optimized formulations will be needed to achieve protective tissue concentrations.


Assuntos
Fármacos Anti-HIV , Infecções por HIV , Humanos , Masculino , Feminino , Lubrificantes/uso terapêutico , Estudos Cross-Over , Pirimidinas/farmacocinética , Inibidores da Transcriptase Reversa/uso terapêutico , Géis , Infecções por HIV/tratamento farmacológico , Infecções por HIV/prevenção & controle
14.
Int J Mol Sci ; 23(19)2022 Oct 08.
Artigo em Inglês | MEDLINE | ID: mdl-36233262

RESUMO

Mesothelial cells (MCs) play a classic role in maintaining homeostasis in pleural, peritoneal, and pericardial cavities. MCs work as lubricants to reduce friction between organs, as regulators of fluid transport, and as regulators of defense mechanisms in inflammation. MCs can differentiate into various cells, exhibiting epithelial and mesenchymal characteristics. MCs have a high potential for differentiation during the embryonic period when tissue development is active, and this potential decreases through adulthood. The expression of the Wilms' tumor suppressor gene (Wt1), one of the MC markers, decreased uniformly and significantly from the embryonic period to adulthood, suggesting that it plays a major role in the differentiation potential of MCs. Wt1 deletion from the embryonic period results in embryonic lethality in mice, and even Wt1 knockout in adulthood leads to death with rapid organ atrophy. These findings suggest that MCs expressing Wt1 have high differentiation potential and contribute to the formation and maintenance of various tissues from the embryonic period to adulthood. Because of these properties, MCs dynamically transform their characteristics in the tumor microenvironment as cancer-associated MCs. This review focuses on the relationship between the differentiation potential of MCs and Wt1, including recent reports using lineage tracing using the Cre-loxP system.


Assuntos
Lubrificantes , Pleura , Adulto , Animais , Diferenciação Celular , Humanos , Camundongos
15.
Int J Mol Sci ; 23(19)2022 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-36233300

RESUMO

The quality of the lubricant between cartilaginous joint surfaces impacts the joint's mechanistic properties. In this study, we define the biochemical, ultrastructural, and tribological signatures of synovial fluids (SF) from patients with degenerative (osteoarthritis-OA) or inflammatory (rheumatoid arthritis-RA) joint pathologies in comparison with SF from healthy subjects. Phospholipid (PL) concentration in SF increased in pathological contexts, but the proportion PL relative to the overall lipids decreased. Subtle changes in PL chain composition were attributed to the inflammatory state. Transmission electron microscopy showed the occurrence of large multilamellar synovial extracellular vesicles (EV) filled with glycoprotein gel in healthy subjects. Synovial extracellular vesicle structure was altered in SF from OA and RA patients. RA samples systematically showed lower viscosity than healthy samples under a hydrodynamic lubricating regimen whereas OA samples showed higher viscosity. In turn, under a boundary regimen, cartilage surfaces in both pathological situations showed high wear and friction coefficients. Thus, we found a difference in the biochemical, tribological, and ultrastructural properties of synovial fluid in healthy people and patients with osteoarthritis and arthritis of the joints, and that large, multilamellar vesicles are essential for good boundary lubrication by ensuring a ball-bearing effect and limiting the destruction of lipid layers at the cartilage surface.


Assuntos
Cartilagem Articular , Vesículas Extracelulares , Osteoartrite , Glicoproteínas/análise , Humanos , Lubrificantes , Fosfolipídeos/análise , Líquido Sinovial/química
16.
Sex Med Rev ; 10(4): 520-528, 2022 10.
Artigo em Inglês | MEDLINE | ID: mdl-36210093

RESUMO

INTRODUCTION: Female sexual dysfunction (SD) is an under-recognized and undertreated problem in patients with systemic autoimmune rheumatic disorders (SARDs). OBJECTIVES: To summarize and evaluate the existing treatment modalities for SD in females with SARDs. METHODS: A systematic review was conducted following the PRISMA guidelines. Electronic databases were searched up to April 2022 for studies that assessed the use of pharmacological and non-pharmacological treatment modalities for the management of SD in females with SARDs. Randomized and observational studies were included. (PROSPERO: CRD42022296381). RESULTS: Seven studies with 426 females with SD were included. Seven different treatment modalities belonging to 5 different classes (androgen therapy, phosphodiesterase-5 inhibitors, exercise, education and local creams) were evaluated in patients with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis. The majority of the studies were of low methodological quality. Standardized patient education and 8-week aerobic walking programs were successful in improving female SD. Local creams improved dyspareunia in females with systemic sclerosis. Testosterone did not significantly improve SD in patients with systemic lupus erythematosus. Accordingly, tadalafil did not result in a significant improvement of SD in females with systemic sclerosis, based on the Female Sexual Function Index. CONCLUSION: There is a lack of sufficient evidence to recommend a certain management strategy for SD in females with SARDs. Nonpharmacological therapy and lubricant creams may be beneficial in females with SARDs. No benefit was demonstrated after androgen therapy or tadalafil. Still, no definite conclusions can be drawn due to the important limitations of the available literature. Overall, our results may be considered preliminary and further research in the field is mandatory. Baniotopoulos P, Pyrgidis N, Minopoulou I, et al. Treatment of Sexual Dysfunction in Women with Systemic Autoimmune Rheumatic Disorders: A Systematic Review. Sex Med Rev 2022;10:520-528.


Assuntos
Lúpus Eritematoso Sistêmico , Escleroderma Sistêmico , Disfunções Sexuais Fisiológicas , Androgênios , Nucleotídeo Cíclico Fosfodiesterase do Tipo 5 , Feminino , Humanos , Lubrificantes , Lúpus Eritematoso Sistêmico/complicações , Lúpus Eritematoso Sistêmico/terapia , Disfunções Sexuais Fisiológicas/etiologia , Disfunções Sexuais Fisiológicas/terapia , Tadalafila , Testosterona
17.
Anal Chem ; 94(42): 14761-14768, 2022 10 25.
Artigo em Inglês | MEDLINE | ID: mdl-36215703

RESUMO

Antibody drugs have been rapidly developed to cure many diseases including COVID-19 infection. Silicone oil is commonly used as a lubricant coating material for devices used in the pharmaceutical industry to store and administer antibody drug formulations. However, the interaction between silicone oil and antibody molecules could lead to the adsorption, denaturation, and aggregation of antibody molecules, impacting the efficacy of antibody drugs. Here, we studied the molecular interactions between antibodies and silicone oil in situ in real time. The effect of the surfactant on such interactions was also investigated. Specifically, the adsorption dynamics of a bispecific antibody (BsAb) onto a silicone oil surface without and with different concentrations of the surfactant PS80 in antibody solutions were monitored. Also the possible lowest effective PS80 concentrations that can prevent the adsorption of BsAb as well as a monoclonal antibody (mAb) onto silicone oil were measured. It was found that different concentrations of PS80 are required for preventing the adsorption of different antibodies. Both BsAB and mAB denature on silicone oil without a surfactant. However, for a low surfactant concentration in the solution, although the surfactant could not completely prevent the antibody from adsorption, it could maintain the native structures of adsorbed BsAb and mAb antibodies on silicone oil. This is important knowledge, showing that to prevent antibody aggregation on silicone oil it is not necessary to add surfactant to a concentration high enough to completely minimize protein adsorption.


Assuntos
Anticorpos Biespecíficos , COVID-19 , Humanos , Óleos de Silicone/química , Tensoativos/química , Excipientes/química , Adsorção , Anticorpos Monoclonais/química , Lubrificantes
18.
Colloids Surf B Biointerfaces ; 220: 112878, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36215899

RESUMO

Slippery liquid-filled porous surfaces (SLIPS) have attracted extensive research attention for their unique repellent properties, but such surfaces typically lack essential bactericidal activity and cannot defend against the spread of bacteria once bacterial contamination occurs. Herein, a slippery liquid-infused porous surface (SLIPS), endowed with both super-repellent and contact-killing antimicrobial performances is reported. Firstly, polystyrene (PS) based porous structures are developed via a facile microphase separation technique with poly(ethylene glycol) (PEG) as the sacrifice template. The porous surface was then covalently modified by 3-(trimethoxysilyl)propyl dimethyl undecyl ammonium chloride (QAC-Silane) to get the contact-killing antimicrobial performances. After lubricant (silicone oil) is introduced to the porous structure, the SLIPS surface demonstrates remarkably high super-repellence against both Gram-positive and negative bacteria, and also maintains essential contact-killing antimicrobial activities from the fixed QAC-11 groups, once the infused lubricant was depleted. Also, this surface demonstrates a reduced coefficient of friction (COF) of ∼56% as compared to that of the control flat surface. Moreover, this SLIPS surface can be easily realized on various substrates, such as silica glass, polycarbonate (PC), polyethylene terephthalate (PET), polyethylene (PE) and silicone catheter tube. Owing to its simple, low-cost and fast fabrication approach, this kind of surface may find unique biomedical applications where an effective antibacterial performance and lubricity are highly needed.


Assuntos
Antibacterianos , Lubrificantes , Porosidade , Propriedades de Superfície , Lubrificantes/farmacologia , Lubrificantes/química , Antibacterianos/farmacologia , Fricção , Bactérias
19.
J Vet Intern Med ; 36(6): 1974-1980, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36181381

RESUMO

BACKGROUND: Fecal specimens are critical for disease screening, diagnosis, and gut microbiome research. For domestic cats, lubricants are often necessary to obtain a sufficient quantity of sample. However, the effect of lubrication on feline microbiome analysis has not been assessed. OBJECTIVES: To evaluate if lubrication using mineral oil during cat feces sample collection affects the DNA extraction, metagenomic sequencing yield, and the microbial composition and diversity in subsequent gut microbiome analyses. ANIMALS: Eight 6-year-old male, neutered, domestic short-haired cats housed in a research facility. METHODS: Cohort study. The gut microbiomes were investigated for fecal sample collection with and without lubrication using whole-genome shotgun metagenomic sequencing. RESULTS: Fecal specimens were collected using a fecal loop under sedation without lubrication and with mineral oil lubrication. There were no significant differences between the 2 groups in the microbial DNA yield in ng/mg fecal sample (75.75 [25.8-125.7] vs 60.72 [33.49-87.95], P = .95), metagenomic sequencing yield in Gbp (10.31 [6.29-14.32] vs 13.53 [12.04-15.02], P = .2), proportion of host contamination (0.1 [0.02-0.18] vs 0.15 [0-0.3], P = .84), relative taxonomy abundance (P > .8), or the number of microbial genes covered (408 132 [341 556-474 708] vs 425 697 [358 505-492 889], P = .31). CONCLUSIONS AND CLINICAL IMPORTANCE: Fecal sampling with mineral oil lubrication did not change the microbial DNA extraction yield, metagenomic sequencing yield, level of host contamination, the microbial composition and diversity in subsequent gut microbiome analyses. Here we reported a proven cat-friendly protocol for fecal sample collection in clinical and research setting for gut microbiome analyses.


Assuntos
Microbiota , Óleo Mineral , Masculino , Gatos , Animais , Lubrificantes , Estudos de Coortes , Fezes , DNA , RNA Ribossômico 16S/genética
20.
Sci Rep ; 12(1): 15926, 2022 09 23.
Artigo em Inglês | MEDLINE | ID: mdl-36151252

RESUMO

To describe the experience in a recently created ocular graft-versus-host disease unit in a tertiary hospital and to detail ocular surface features and complications after allogeneic hematopoietic stem cell transplantation (allo-HSCT). This retrospective study included all patients who underwent allo-HSCT, with or without chronic GVHD and were being monitored in the Hematopoietic Stem Cell Transplantation Unit in the UNICAMP Clinical Hospital (Campinas, Sao Paulo, Brazil) from 2015 to 2020. Patients were concomitantly evaluated by hematology and ophthalmology teams of the Ocular GVHD Unit. Hematologists performed a comprehensive systemic evaluation searching and grading mouth, skin, lungs, gastrointestinal tract, liver and genitalia GVHD. While ophthalmologists evaluated ocular symptoms through specific questionnaire (Ocular Surface Disease Index-OSDI) and a protocol of distinct ocular surface parameters for dry eye disease (1) and ocular complications, which encompassed meniscometry, non-invasive tear break-up time (NITBUT) measurement, conjunctival hyperemia quantification, meibography, fluorescein and lissamine staining and Schirmer's test. Patients were diagnosed with chronic GVHD using the National Institutes of Health (NIH) Consensus Criteria for Chronic Graft-versus-Host Disease. The International Chronic Ocular GVHD Consensus Group (ICOGCG) score was obtained at the onset of ocular disease presentation or afterwards. A total of 82 patients underwent allo-HSCT (97.6% full matched and 2.4% haploidentical), mainly for cases of leukemia and 73.2% had chronic GVHD. Mean onset time for chronic GVHD was 232 ± 7.75 days. The mouth, skin, and eyes were the main organs involved (63%, 50%, and 48%, respectively). Symptom scores and all ocular surface parameters differ in patients with and without chronic GVHD and along different timepoints of the follow-up. Ocular complications mostly involved were severe DED and meibomian gland dysfunction, conjunctival scarring, cataract and infections resulting in keratitis and corneal perforation. As therapeutic strategies, 73% patients received preservative-free lubricants, 27% autologous serum, 48% topical steroids, 27% oral tetracycline derivatives, 22% mucolytic eye drops and 3 patients needed bandage contact lens. Ocular GVHD is a complex and challenging disease with varied manifestations, resulting in a broad range of ocular test endpoints, and inconsistent treatment responses. The main ocular presentations were dry eye, meibomian gland dysfunction and cataracts. The therapeutic approach often involves topical steroids and autologous serum tears. It is important to monitor these patients closely, so the ocular GVHD Unit may improve the care, providing prompt identification of ocular manifestations and faster treatment of complications.


Assuntos
Síndromes do Olho Seco , Doença Enxerto-Hospedeiro , Transplante de Células-Tronco Hematopoéticas , Disfunção da Glândula Tarsal , Brasil , Síndromes do Olho Seco/diagnóstico , Síndromes do Olho Seco/etiologia , Síndromes do Olho Seco/terapia , Expectorantes/uso terapêutico , Fluoresceína/metabolismo , Doença Enxerto-Hospedeiro/diagnóstico , Doença Enxerto-Hospedeiro/tratamento farmacológico , Doença Enxerto-Hospedeiro/etiologia , Transplante de Células-Tronco Hematopoéticas/efeitos adversos , Humanos , Lubrificantes/uso terapêutico , Soluções Oftálmicas/uso terapêutico , Estudos Retrospectivos , Tetraciclinas/uso terapêutico , Transtornos da Visão/complicações
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