Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 235.809
Filtrar
2.
PLoS Comput Biol ; 16(9): e1008095, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32881868

RESUMO

Research publications and data nowadays should be publicly available on the internet and, theoretically, usable for everyone to develop further research, products, or services. The long-term accessibility of research data is, therefore, fundamental in the economy of the research production process. However, the availability of data is not sufficient by itself, but also their quality must be verifiable. Measures to ensure reuse and reproducibility need to include the entire research life cycle, from the experimental design to the generation of data, quality control, statistical analysis, interpretation, and validation of the results. Hence, high-quality records, particularly for providing a string of documents for the verifiable origin of data, are essential elements that can act as a certificate for potential users (customers). These records also improve the traceability and transparency of data and processes, therefore, improving the reliability of results. Standards for data acquisition, analysis, and documentation have been fostered in the last decade driven by grassroot initiatives of researchers and organizations such as the Research Data Alliance (RDA). Nevertheless, what is still largely missing in the life science academic research are agreed procedures for complex routine research workflows. Here, well-crafted documentation like standard operating procedures (SOPs) offer clear direction and instructions specifically designed to avoid deviations as an absolute necessity for reproducibility. Therefore, this paper provides a standardized workflow that explains step by step how to write an SOP to be used as a starting point for appropriate research documentation.


Assuntos
Métodos , Registros , Redação/normas , Documentação , Humanos , Reprodutibilidade dos Testes , Projetos de Pesquisa/normas , Fluxo de Trabalho
3.
Goiânia; SES-GO; 20 ago 2020. ilus, graf, tab, mapas.(Boletim Epidemiológico Covid-19, 20).
Monografia em Português | Coleciona SUS, CONASS, SES-GO | ID: biblio-1117607

RESUMO

A Secretaria de Estado da Saúde de Goiás através da Superintendência de Vigilância em Saúde e Gerência de Vigilância Epidemiológica, apresentam neste boletim a distribuição de casos confirmados, óbitos e taxa de crescimento de COVID-19 no mundo, no Brasil, no período compreendido entre dezembro de 2019 a 15 de agosto de 2020. Quanto ao Estado de Goiás apresenta a distribuição dos casos notificados de COVID-19 segundo classificação e critério de confirmação, no período de 04 de fevereiro a 15 de agosto de 2020, a distribuição dos casos confirmados por data de início de sintomas, do acumulado de casos confirmados, dos casos confirmados e média móvel segundo a data de início de sintomas, do acumulado de óbitos, dos óbitos confirmados e média móvel segundo a data de ocorrência do óbito, da distribuição proporcional dos casos confirmados por municípios integrantes da Região metropolitana, do interior e capital, dos casos confirmados e taxa de incidência por município de residência, de casos confirmados por Semana Epidemiológica nas Macrorregiões, distribuição de casos confirmados de COVID-19 por Semana Epidemiológica segundo a região de saúde, número de casos confirmados segundo gênero, faixa etária e taxa de incidência, percentual de casos confirmados segundo raça/cor, casos confirmados segundo evolução, percentual de casos confirmados segundo ocupação, distribuição por município de residência dos óbitos confirmados segundo taxa de letalidade, percentual dos óbitos confirmados por sexo, óbitos confirmados e letalidade segundo faixa etária, casos confirmados que necessitaram de hospitalização segundo a evolução, perfil de gestantes com COVID-19, distribuição dos casos confirmados em gestantes segundo a necessidade de hospitalização, dos casos confirmados em gestantes segundo o local de internação, dos casos confirmados em gestantes segundo evolução, casos confirmados que necessitaram de hospitalização segundo o tempo médio de internação, percentual de casos confirmados por critério laboratorial segundo o método diagnóstico e positividade dos testes RT-PCR liberados pelo LACEN-GO segundo identificação de SARS-CoV-2


The Goiás State Department of Health, through the Health Surveillance Superintendence and Epidemiological Surveillance Management, presents in this bulletin the distribution of confirmed cases, deaths and growth rate of COVID-19 in the world, in Brazil, in the period between December from 2019 to August 15, 2020. As for the State of Goiás, it presents the distribution of notified cases of COVID-19 according to classification and confirmation criteria, in the period from February 4 to August 15, 2020, the distribution of cases confirmed by date of onset of symptoms, cumulative number of confirmed cases, confirmed cases and moving average according to date of symptom onset, accumulated number of deaths, confirmed deaths and moving average according to date of occurrence of death, proportional distribution of cases confirmed by municipalities in the metropolitan region, inland and capital, of confirmed cases and incidence rate by municipality of residence, of case s confirmed by Epidemiological Week in Macroregions, distribution of confirmed cases of COVID-19 by Epidemiological Week according to health region, number of confirmed cases according to gender, age group and incidence rate, percentage of confirmed cases according to race / color, confirmed cases according to evolution, percentage of confirmed cases according to occupation, distribution by municipality of residence of deaths confirmed according to lethality rate, percentage of deaths confirmed by sex, confirmed deaths and lethality according to age group, confirmed cases that required hospitalization according to the evolution, profile of pregnant women with COVID-19, distribution of confirmed cases in pregnant women according to the need for hospitalization, of confirmed cases in pregnant women according to the place of hospitalization, of the confirmed cases in pregnant women according to the evolution, confirmed cases that required hospitalization according to the average length of hospitalization, percentage of confirmed cases by laboratory criteria according to the diagnostic method and positivity of RT-PCR tests released by LACEN-GO according to the identification of SARS-CoV-2


Assuntos
Humanos , Masculino , Feminino , Gravidez , Lactente , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Adulto Jovem , Sinais e Sintomas , Vigilância Sanitária , Brasil/epidemiologia , Incidência , Mortalidade , Infecções por Coronavirus/epidemiologia , Vigilância em Saúde Pública , Monitoramento Epidemiológico , Betacoronavirus , Grupos Etários , Alta do Paciente , Pneumonia Viral , Prevalência , Notificação de Doenças , Gestantes , Grupos de Populações Continentais , Diagnóstico , Economia , Distribuição de Produtos , Hospitalização , Laboratórios , Métodos , Ocupações
4.
Cuad. Hosp. Clín ; 61(1): [17], jul. 2020. ilus.
Artigo em Espanhol | LILACS, LIBOCS | ID: biblio-1118869

RESUMO

OBJETIVO: Probar una metodología de enseñanza-aprendizaje, instrumentos de medición y sistema de implementación de la ECOE en relación con lactancia materna, alimentación complementaria, crecimiento y consejería. MATERIAL Y MÉTODOS: Se estudió la adquisición de competencias sobre alimentación en menores de dos años en internos de pediatría aplicando la evaluación clínica objetiva estructurada (ECOE) antes y después del desarrollo de un proceso de enseñanza aprendizaje (PEA) estructurado. Se organizaron cuatro estaciones de evaluación de los aspectos centrales de alimentación y crecimiento, en un grupo de internos seleccionados al azar. RESULTADOS: Las cuatro estaciones de la ECOE se aplicaron sin dificultades antes y después del PEA. Los resultados mostraron una mejora en el rendimiento de los internos, de manera individual y de grupo; en este último las diferencias en la media fueron para alimentación complementaria pre 2,5 (DE 0,93) y post 5 (DE 2,39); consejería pre 5,75 (DE 1,49) y post 8,13 (DE 1,25); lactancia materna pre 12,63 (DE 2,5) y post 16,38 (DE 2) y velocidad de crecimiento pre 3,13 (DE 1,36) y post 3,38 (DE 0,92). Los resultados fueron estadísticamente significativos para los tres primeros rubros. CONCLUSIONES: En base a estos resultados se sugieren mejoras en el programa de enseñanza y se verifica la aplicabilidad de la ECOE en el internado del Hospital del Niño Dr. Ovidio Aliaga Uría.


OBJECTIVE: To test a teaching-learning methodology, measurement tools and OSCE implementation system in relation to breastfeeding, complementary feeding, growth and counseling. METHODOLOGY: The acquisition of competences was studied by applying objective structured clinical examination (OSCE) before and after the development of a structured teaching-learning process. Four assessment stations were organized considering central aspects on feeding and growth of children under two years of age, in a group of randomly selected students during medical internship. RESULTS: The four OSCE stations were applied without difficulties before and after the learning and teaching process. The results showed an improvement in the performance of interns, individually and in groups; in the latter, mean differences were: for complementary feeding pre 2.5 (SD 0.93) and post 5 (SD 2.39); counseling pre 5.75 (SD 1.49) and post 8.13 (SD 1.25); breastfeeding pre 12.63 (SD 2.5) and post 16.38 (SD 2) and growth velocity pre 3.13 (SD 1.36) and post 3.38 (SD 0.92). The results were statistically significant for the first three items. CONCLUSIONS: Based on these results, the authors suggest improvements in the teaching program, and verify the applicability of the OSCE for the evaluation of rotatory internship at the Hospital del Nino Dr. Ovidio Aliaga Uria.


Assuntos
Humanos , Lactente , Aleitamento Materno , Menores de Idade , Internato e Residência , Ensino , Aprendizagem , Métodos
5.
Rev. bras. ciênc. mov ; 28(1): 1-23, jan.-mar., 2020. ilus
Artigo em Português | LILACS | ID: biblio-1102283

RESUMO

O presente estudo teve como objetivo descrever e analisar uma experiência pedagógica no Ensino Médio em aulas de Educação Física a partir dos Jogos Esportivos Coletivos (JEC). As aulas de Educação Física no Ensino Médio, geralmente apresentam alguns problemas pertinentes, tais como: privilégios aos alunos mais habilidosos em detrimento dos demais; conteúdos repetitivos; predomínio de competição exacerbada e, em alguns casos, mínima intervenção pedagógica. Como procedimento metodológico, realizamos pesquisa bibliográfica e pesquisa de campo, de caráter participante, sendo este um estudo qualitativo, que teve como técnica a utilização de questionários e diário de campo. A experiência foi realizada ao longo de 8 (oito) aulas, em uma turma de 2º ano do Ensino Médio, composta por 7 (sete) meninos e 11 (onze) meninas. Como resultado, consideramos que a abordagem sistêmica dos JEC possui uma interface com a educação para o lazer e o referencial cultural, podendo auxiliar na minimização de problemas existentes nas aulas como exclusão, desânimo, individualismo e mínima aprendizagem. Pudemos observar no início da experiência pedagógica uma estrutura social que era movida em torno dos mais habilidosos, que aos poucos foi se modificando com a intervenção pedagógica que utilizou o ensino sistêmico dos JEC. Ao oportunizar esse modelo de ensino, as aulas se tornaram diferentes, divertidas e menos excludentes, demonstrando a relevância de uma mediação pedagógica no Ensino Médio que: a) considere o contexto cultural dos educandos; b) valorize atividades que exploram o componente lúdico; c) oportunize diferentes experiências na esfera da cultura corporal de movimento; d) utilize o ensino sistêmico dos JEC na aprendizagem de uma modalidade esportiva e; e) contribua na formação de praticantes e espectadores críticos...(AU)


The present study aimed to describe and analyze a pedagogical experience in High School in Physical Education classes from the Collective Sports Games (JEC). Physical Education classes in High School generally present some pertinent problems, such as privileges to the most skilled students to the detriment of others, repetitive content, predominance of exacerbated competition and, in some cases, minimal pedagogical intervention. As a methodological procedure, we carried out bibliographic research and field research, of the participant character, being a qualitative study, which had as technique the use of questionnaires and field diary. The experiment was carried out during 8 (eight) classes, in a class of 2nd year of High School, composed of 7 (seven) boys and 11 (eleven) girls. As a result, we consider that the systemic approach of JEC has an interface with leisure education and cultural reference, and can help to minimize problems in class such as exclusion, discouragement, individualism and minimal learning. We could observe at the beginning of the pedagogical experience, a social structure that was moved around the most skilled, that gradually was changing with the pedagogical intervention that used the systemic teaching of the JEC. When we worked this teaching model, the classes became different, fun and less exclusionary, demonstrating the relevance of a pedagogical mediation in High School that: a) considers the cultural context of the students; b) value activities that explore the recreational component; c) give different experiences in the sphere of body culture of movement; d) use the JEC systemic teaching in the learning of a sports modality and e) contribute in the formation of critical practitioners and spectators...(AU)


Assuntos
Humanos , Masculino , Feminino , Educação Física e Treinamento , Aula , Ensino Fundamental e Médio , Atividades de Lazer , Esportes , Estudantes , Ensino , Educação , Aprendizagem , Métodos , Movimento
6.
Psicol. ciênc. prof ; 40: 1-17, jan.-maio 2020. tab, graf
Artigo em Português | LILACS, Index Psicologia - Periódicos técnico-científicos | ID: biblio-1100392

RESUMO

No escopo da investigação de sob quais condições ocorre a aprendizagem e a emergência de operantes verbais, diferentes condições de ensino têm sido planejadas. Considerando que a população com Transtorno do Espectro Autista (TEA) apresenta frequentemente um repertório de operantes verbais ausente ou fracamente estabelecido, é necessário o estudo do planejamento de intervenções sistemáticas desses repertórios com condições de favorecer não só a aquisição de vocabulário, mas também o seu potencial gerativo de novas funções verbais. O Multiple Exemplar Instruction (MEI) é uma estrutura de ensino que tem demonstrado resultados promissores pela sua capacidade de estabelecer relações entre comportamentos de ouvinte e de falante e gerar novas respostas verbais. O objetivo deste estudo foi verificar os efeitos do MEI sobre o estabelecimento e integração entre os repertórios de ouvinte e de falante (ecoico, tato e mando). Participaram duas crianças com TEA, com idades de 7 e 8 anos e cuja comunicação era muito restrita e baseada em trocas de figuras. O ensino adotou três conjuntos com três estímulos cada. O ensino com cada conjunto foi realizado separadamente. Ora o ensino consistia no treino de ouvinte baseado em seleção, ora tentativas de ouvinte, ecoico, tato e mando, eram apresentadas de forma rotativa. Sondas múltiplas intercalaram os ensinos e verificaram os efeitos destes sobre o repertório de falante com os demais conjuntos. Os resultados demonstraram um aumento na emissão de respostas de ouvir e falar após o ensino por MEI para os dois participantes, ambos com repertório verbal restrito, mas o procedimento foi mais efetivo para uma das crianças...(AU)


In the scope of research about under what conditions the learning and emergency of verbal operants occurs, different teaching conditions have been planned. Considering that the population with Autistic Spectrum Disorder (ASD) often has a repertoire of verbal operants absent or poorly established, it is necessary to study the planning of systematic interventions in these repertoires with conditions to favor not only vocabulary acquisition, but also their generative potential of new verbal functions. The Multiple Exemplar Instruction (MEI) is a teaching structure that has shown promising results for its ability to establish relationships between listening and speaker behaviors and to generate new verbal responses. The objective of this study was to verify the effects of the MEI on the establishment and integration between the listener repertoires and the speaker (echoic, tact and mand). Two children with ASD, aged between 7 and 8 years old, participated in the study, whose communication was very restricted and based on exchanges of figures. The teaching adopted three sets with three stimuli each. Teaching with each set was carried out separately. The teaching consisted of the training of listener based on selection, sometimes attempts of listener, echoic, tact and mand, were presented in a rotating way. Multiple probes intercalated the teachings and verified the effects of these on the repertoire of speaker with the other sets. The results showed an increase in the emission of listening and speaking responses after MEI teaching for the two participants, both with restricted verbal repertoire, but the procedure was more effective for one of the children...(AU)


El el ámbito de la investigación de en qué condiciones se produce el aprendizaje y la aparición de operantes verbales, se han planificado diferentes condiciones de enseñanza. Teniendo en cuenta que la población con Trastorno del Espectro Autista (TEA) con frecuencia presenta un repertorio de operantes verbales ausentes o débilmente establecidos, es necesario estudiar la planificación de intervenciones sistemáticas en estos repertorios con condiciones para favorecer no solo la adquisición de vocabulario, sino también su potencial generativo de nuevas funciones verbales. La Instrucción Ejemplar Múltiple (MEI) es una estructura de enseñanza que ha mostrado resultados prometedores por su capacidad para establecer relaciones entre los comportamientos del oyente y el hablante y generar nuevas respuestas verbales. El objetivo de este estudio fue verificar los efectos del MEI en el establecimiento e integración entre los repertorios de oyentes y hablantes (eco, tacto y comando). Participaron dos niños con TEA, de 7 y 8 años, cuya comunicación era muy restringida y basada en intercambios de figuras. La enseñanza adoptó tres conjuntos con tres estímulos cada uno. La enseñanza con cada conjunto se llevó a cabo por separado. Algunas veces la enseñanza consistía en entrenar al oyente en base a la selección, a veces los intentos de escuchar, eco, tacto y comando, se presentaban de manera rotativa. Múltiples sondas intercalan las enseñanzas y verifican sus efectos en el repertorio de los hablantes con los otros grupos. Los resultados mostraron un aumento en la emisión de respuestas para escuchar y hablar después de la enseñanza de MEI para ambos participantes, ambos con repertorio verbal restringido, pero el procedimiento fue más efectivo para uno de los niños...(AU)


Assuntos
Humanos , Masculino , Criança , Transtorno Autístico , Ensino , Comportamento Verbal , Transtorno do Espectro Autista , Aprendizagem , População , Vocabulário , Comportamento , Comunicação , Tutoria , Métodos
7.
Rev Lat Am Enfermagem ; 28: e3269, 2020.
Artigo em Português, Espanhol, Inglês | MEDLINE | ID: mdl-32401899

RESUMO

OBJECTIVE: to validate an educational booklet for people with intestinal stoma as a technological resource in the teaching of self-care. METHOD: a methodological research for the construction and validation of an educational booklet by nine expert judges and 25 people with stomas. The agreement index of at least 80% was considered to guarantee the validation of the material. RESULTS: regarding the objectives of the booklet, all the judges evaluated the items as "adequate" or "totally adequate", with a content validity index of 1.00. Regarding the structure and presentation of the booklet, the total index was 0.84. Regarding relevance, the total was 0.97 and the general index of the educational booklet was 0.89, confirming the validation with the judges. All items of the organization, writing style, appearance and motivation of the material were considered as validated by the target audience, reaching a total agreement index of 0.99. CONCLUSION: in the context of health education, the booklet was considered valid and suitable for the care of people with intestinal stoma, and can be used in teaching, research, extension and care for people with intestinal stoma.


Assuntos
Enterostomia/educação , Educação em Saúde/normas , Manuais como Assunto/normas , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Métodos , Pessoa de Meia-Idade , Educação de Pacientes como Assunto/métodos , Reprodutibilidade dos Testes , Autocuidado/instrumentação , Inquéritos e Questionários , Adulto Jovem
8.
Nat Methods ; 17(2): 117, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32020102
9.
PLoS One ; 15(2): e0223700, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32092080

RESUMO

Emulsified volatile anesthetic can be directly injected into the circulation and eliminated from blood through lungs. Taking advantage of the unique pharmacokinetics of the emulsified volatile anesthetics, we aimed to develop a less traumatic method to differentially deliver them to the spinal cord of rabbit. Sixteen New Zealand White rabbits were randomly assigned to the isoflurane or sevoflurane group. A catheter was placed into the descending aorta, and emulsified isoflurane (8mg/kg/h) or sevoflurane (12mg/kg/h) was given respectively. The concentration and partial pressure of the anesthetics in the jugular and femoral vein were measured. Our results showed that the partial pressure for isoflurane was 3.91±1.11 mmHg and 12.61±1.60 mmHg (1.0MAC), and for sevoflurane was 3.89±1.00 mmHg and 19.92±1.84mmHg (1.0MAC), in the jugular vein and femoral vein, respectively. There was significant difference between jugular and femoral vein partial pressure for both isoflurane and sevoflurane groups (both P < 0.001). In conclusion, a simple and minimally invasive method has been successfully developed to selectively deliver isoflurane and sevoflurane to the spinal cord in the rabbit. Before the anesthetics taking action on the brain, 69% of isoflurane and 81% of sevoflurane were removed through lungs. This method can be used to investigate sites and mechanisms of volatile anesthetic action.


Assuntos
Anestésicos Inalatórios/administração & dosagem , Medula Espinal/metabolismo , Anestésicos Inalatórios/farmacocinética , Animais , Encéfalo/efeitos dos fármacos , Emulsões , Veia Femoral , Isoflurano/administração & dosagem , Veias Jugulares , Pulmão/metabolismo , Métodos , Pressão Parcial , Coelhos , Sevoflurano/administração & dosagem , Volatilização
10.
Biochim Biophys Acta Biomembr ; 1862(6): 183214, 2020 06 01.
Artigo em Inglês | MEDLINE | ID: mdl-32081704

RESUMO

Structural data on membrane proteins in a lipid membrane environment is challenging to obtain but needed to provide information on the, often essential, protein-lipid interplay. A common experimental bottleneck in obtaining such data is providing samples in sufficient amounts and quality required for structural studies. We developed a new production protocol for the single-pass transmembrane protein (SPTMP) tissue factor (TF), exploiting the high expression level in E. coli inclusion bodies and subsequent refolding. This provided more than 5 mg of functional TF per liter bacterial culture. This is substantially more than what was obtained by the classical approaches for expressing TF in the membrane-anchored configuration. We optimized reconstitution into circularized nanodiscs enabling the formation of stable, TF loaded nanodiscs with different lipid compositions and with a limited material waste. The blood coagulation cascade is initiated by the complex formation between TF and Factor VIIa (FVIIa), and we probed this interaction by a functional assay and SPR measurements, which revealed similar activity and binding kinetics as TF produced by other protocols, demonstrating that high-yield production does not compromise TF function. Furthermore, the amounts of sample produced permitted initial small angle X-ray scattering studies providing the first structural information about TF and its binding to FVIIa in a lipid environment. This strategy possibly allows for probing the multicomponent complex TF:FVIIa together with its substrate Factor X on a lipid bilayer, but may also be relevant as a production strategy for other SPTMP for which structural information, in general, is limited.


Assuntos
Fator VIIa/metabolismo , Bicamadas Lipídicas/química , Complexos Multiproteicos/química , Dobramento de Proteína , Tromboplastina/química , Animais , Escherichia coli/citologia , Escherichia coli/metabolismo , Fator X/metabolismo , Humanos , Corpos de Inclusão/metabolismo , Métodos , Nanoestruturas , Ligação Proteica , Tromboplastina/metabolismo
12.
Chemosphere ; 245: 125671, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31883501

RESUMO

Determining bioavailable trace concentrations of mercury (Hg) in water is still a challenging analytical task. In this study, we report a methodology for determining labile Hg in natural waters using newly developed sorbents. Silicon dioxide at a nanoparticle range (Si-np) and cellulose powder at a microparticle range (Cel-p), both modified with the ionic liquid trioctylmethylammonium thiosalicylate (TOMATS), have been tested as sorbents (sorb-TOMATS) for Hg(II) uptake from solution. These novel sorb-TOMATS materials were characterized, and parameters affecting the uptake were examined. A similar Hg(II) uptake efficiency (97%) and binding capacity (9 mg Hg/g) was obtained for both sorb-TOMATS, while only a 25% of Hg(II) was taken up using non-impregnated materials. Moreover, these sorb-TOMATS were effectively embedded in agarose gel and were tested as a novel binding phase for the Diffusive Gradients in Thin Films (DGT) technique. Research revealed Si(np)-TOMATS sorbent as a suitable binding phase in the DGT technique for Hg(II) measurements, since it also allowed the efficient elution of the bound Hg(II). This new binding phase showed strong linear correlation between the accumulated Hg(II) mass and deployment time, which is in agreement with the DGT principle. In summary, this novel sorbent has a great potential to improve Hg monitoring in natural waters when integrated it in the DGT design.


Assuntos
Monitoramento Ambiental/métodos , Líquidos Iônicos , Mercúrio/análise , Poluentes Químicos da Água/análise , Adsorção , Difusão , Métodos , Polímeros , Compostos de Amônio Quaternário/química
13.
Rev. Soc. Bras. Med. Trop ; 53: e20190378, 2020. tab, graf
Artigo em Inglês | LILACS, SESSP-IALPROD, Sec. Est. Saúde SP | ID: biblio-1092220

RESUMO

Abstract INTRODUCTION Human retroviruses and the hepatitis B and C viruses (HBV and HCV, respectively) share routes of transmission; thus, coinfections occur and could alter subsequent disease outcomes. A preliminary study on human T-lymphotropic virus types 1 and 2 (HTLV-1/2) in serum samples from HBV- and HCV-infected individuals in São Paulo revealed 1.3% and 5.3% rates of coinfection, respectively. These percentages were of concern since they were detected in HTLV-endemic regions and in high-risk individuals in Brazil. The present study was conducted to extend and confirm these data. METHODS HTLV-1/2 and human immunodeficiency virus (HIV) infection status were identified in 1,984 sera for HBV and HCV viral load quantification - 1,290 samples from HBV-infected individuals (53.3% men, mean age: 47.1 years) and 694 samples from HCV-infected individuals (56.3% men, mean age: 50.1 years). HTLV-1/2 antibodies were detected by enzyme immunoassay, followed by western blotting and line immunoassay; HIV infection was detected by enzyme immunoassay. RESULTS HTLV-1/-2 infection was detected in 1.9% HBV-infected individuals (0.7% HTLV-1 and 1.2% HTLV-2) and in 4.0% (2.4% HTLV-1 and 1.6% HTLV-2) HCV-infected individuals; HIV infection was detected in 9.2% and 14.5%, respectively. Strong associations with HTLV and HIV, male sex, and older age were found in HBV/HTLV and HCV/HTLV-coinfected individuals (p<0.05). CONCLUSIONS HTLV-1 and HTLV-2 were confirmed to be prevalent in individuals with HBV and HCV in São Paulo; coinfected individuals deserve further clinical and laboratory investigation.


Assuntos
Sexo , HIV , Carga Viral , Hepatite B , Infecções , Métodos
14.
BMC Res Notes ; 12(1): 806, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31836019

RESUMO

OBJECTIVE: Scrapie is a transmissible spongiform encephalopathy (TSE) that naturally occurs in sheep and goats. This fatal neurodegenerative disease results from misfolding of the normal cellular prion protein (PrPC) to a pathogenic prion protein form (PrPSc). This pathogenic form, PrPSc, accumulates in the brain and lymphoid tissues. The presence of PrPSc can be detected by an in vitro conversion assay known as real-time quaking induced conversion (RT-QuIC). RT-QuIC has been used to detect PrPSc in a variety of biological tissues from brains to fluids. While this technique is both rapid and sensitive, enhancing the detection of prions would be valuable in the diagnostic laboratories. RESULTS: In this study, we assessed whether PrPSc detection sensitivity of RT-QuIC can be increased by enriching PrPSc in scrapie tissue homogenates using commercially available aggregated protein binding ligands coated magnetic beads (PAD-Beads). Coupling of RT-QuIC to PAD-Beads based cleanup allowed detection of PrPSc rapidly and without dilution of scrapie sheep brain homogenates prior to RT-QuIC. The PAD-Beads sample pretreatment step prior to RT-QuIC is a useful enhancement in the diagnosis of TSEs.


Assuntos
Proteínas PrPSc/análise , Scrapie/diagnóstico , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Métodos , Doenças Neurodegenerativas/diagnóstico , Doenças Neurodegenerativas/metabolismo , Scrapie/metabolismo , Ovinos
15.
Estud. pesqui. psicol. (Impr.) ; 18(4): 1087-1105, out.-dez. 2019. tab, ilus
Artigo em Português | LILACS, Index Psicologia - Periódicos técnico-científicos | ID: biblio-994974

RESUMO

Discorre-se sobre o desenvolvimento brasileiro da psicologia do trabalho e das organizações, com o foco na sua formação pós-graduada e produção científica. Inicia-se pela demarcação conceitual. Na seção subsequente, argumenta-se a favor da relação entre o crescimento e a diversificação da subárea e também a expansão da pós-graduação no Brasil. Segue um panorama das revisões de literatura nacionais, visando ressaltar a diversidade na prática de pesquisa. Finaliza-se com considerações sobre os desafios para alcançar níveis mais elevados de amadurecimento científico.(AU)


This article aims to describe the Brazilian development of work and organizational psychology, with a focus on its graduate training and scientific production. It begins by defining concepts. The following section argues in favor of the relationship between growth and diversification of the subarea and the expansion of graduate programs in Brazil. An overview of the national scientific reviews follows, in order to mark the diversity in research practices. Finally, the chapter discusses challenges to achieve higher levels of scientific maturity.(AU)


Se aborda el desarrollo brasileño de la psicología del trabajo y de las organizaciones, con el foco en su formación en el posgrado y producción científica. Se inicia por la demarcación conceptual. En la sección posterior, se argumenta a favor de la relación entre el crecimiento y la diversificación del sub-área y también la expansión del posgrado en el Brasil. Se propone un panorama de las revisiones de literatura nacionales, con vistas a resaltar la diversidad en la práctica de investigación. Se concluye con consideraciones sobre los desafíos para alcanzar niveles más elevados de madurez científica.(AU)


Assuntos
Psicologia , Educação de Pós-Graduação , Psicologia Aplicada , Pesquisa , Pesquisa Científica e Desenvolvimento Tecnológico , Métodos
17.
Enferm. foco (Brasília) ; 10(7): 141-146, dez. 2019.
Artigo em Português | LILACS, BDENF - Enfermagem | ID: biblio-1051472

RESUMO

Objetivo: Descrever o método de desenvolvimento de uma tecnologia educacional, na modalidade de aplicativo-guia, para o manejo do potencial doador pediátrico, realizada como produto do Mestrado Profissional. Metodologia: relato de um método científico aplicado. Utilizou-se os passos propostos por Echer, com a perspectiva de apresentar o percurso para a produção da Tecnologia Educacional. Resultados: A construção está sustentada na Teoria das Necessidades Humanas Básicas, nos Diagnósticos e Intervenções de Enfermagem. Foi realizado mapeamento cruzado, dos indicadores selecionados, a partir das necessidades humanas básicas, decorrentes das alterações fisiopatológicas e as percebidas na avaliação clínica, com os Diagnósticos de Enfermagem, segundo taxonomia de NANDA-I e Intervenções de enfermagem baseadas nos diagnósticos, sob a ótica da manutenção dos órgãos a serem doados. Conclusão: O Aplicativo-guia subsidiará o Enfermeiro no planejamento da assistência de enfermagem segura e propiciará um impacto social no processo de transplantes em pediatria. (AU)


Objective: To describe the method of development of an educational technology as a guide application for the management of potential pediatric donors, carried out as a product of the Professional Master. Methodology: report of an applied scientific method. The steps proposed by Echer5 were used, with the perspective of presenting the path for the production of Educational Technology. Results: It is supported by the Theory of Basic Human Needs, Nursing Diagnoses and Interventions. Cross-mapping of selected indicators was performed based on basic human needs, resulting from pathophysiological changes and those perceived in clinical evaluation, with Nursing Diagnoses, according to NANDA-I taxonomy and Nursing interventions based on nursing diagnoses, under the optics of the maintenance of the organs to be donated. Conclusion: The Guide application will support the Nurse in the planning of safe nursing care and will provide a social impact on the process of transplantation in pediatrics. (AU)


Objetivo: Describir el método de desarrollo de una tecnología educativa como una aplicación de guía para el manejo de posibles donantes pediátricos, realizada como producto del Master Profesional. Metodología: informe de un método científico aplicado. Se utilizaron los pasos propuestos por Echer5, con la perspectiva de presentar el camino para la producción de Tecnología Educativa. Resultados: está respaldado por la Teoría de las necesidades humanas básicas, los diagnósticos e intervenciones de enfermería. El mapeo cruzado de los indicadores seleccionados se realizó con base en las necesidades humanas básicas, como resultado de los cambios fisiopatológicos y los percibidos en la evaluación clínica, con los Diagnósticos de Enfermería, de acuerdo con la taxonomía NANDA-I y las intervenciones de Enfermería basadas en diagnósticos de enfermería, bajo La óptica del mantenimiento de los órganos a donar. Conclusión: La aplicación de la Guía apoyará a la Enfermera en la planificación de la atención de enfermería segura y proporcionará un impacto social en el proceso de trasplante en pediatría. (AU)


Assuntos
Morte Encefálica , Tecnologia Educacional , Métodos , Cuidados de Enfermagem , Processo de Enfermagem
18.
An Acad Bras Cienc ; 91(3): e20190676, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31618414

RESUMO

The aim of this work was to optimize a maceration condition of cannabis (Cannabis sativa L.). A circumscribed central composite experimental design was applied in this work. Temperature and time were varied from 40-80 °C and 30-90 min, respectively. The three responses (i.e., extraction yield, cannabidiol content, and Δ9- tetrahydrocannabinol content) were predicted by computer software. The yield was high when cannabis was macerated using ethanol at high temperature and long duration time. While cannabidiol and Δ9- tetrahydrocannabinol content was high when macerating at a low heating temperature and short duration time. The optimal condition provided the simultaneous high of cannabidiol and Δ9- tetrahydrocannabinol content was 40 °C for 30 min. The prediction was accurate due to low percent error. This optimal condition could be used as a guide for maceration of cannabis to obtain the extract containing a high content of cannabidiol and Δ9- tetrahydrocannabinol.


Assuntos
Canabidiol/análise , Cannabis/química , Dronabinol/isolamento & purificação , Extratos Vegetais/análise , Cromatografia Líquida de Alta Pressão , Dronabinol/análise , Métodos , Temperatura , Fatores de Tempo
19.
Clin Appl Thromb Hemost ; 25: 1076029619872556, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31523979

RESUMO

The effect of direct oral anticoagulants (DOACs) on laboratory tests dependent on the production of their targets, factor IIa and factor Xa (FXa), is a well-known problem and can cause both false positive and negative results. Therefore, the correct interpretation of tests performed in patients receiving DOACs is necessary to avoid misclassification and subsequent clinical consequences. However, even with significant experience, there are situations where it is not possible to assess the influence of some methods. Particularly important is the situation in the diagnosis of lupus anticoagulants using the dilute Russell viper venom timetest, which is based on direct FXa activation. A very promising solution to this situation is offered by the DOAC laboratory balancing procedure DOAC-Stop. For evaluating the effectiveness of this procedure, 60 (20 apixaban, 20 dabigatran, and 20 rivaroxaban) patients treated with DOACs were enrolled. All patient samples were analyzed for the presence of individual DOAC types and subsequently subjected to the DOAC-Stop procedure.We evaluated its effectiveness by our own high-performance liquid chromatography-coupled tandem mass spectrometrymethod, which simultaneously sets all high-sensitivity DOACs. Unlike coagulation tests based on the determination of the residual effects of DOACs on target enzymes, which is complicated by extensive interindividual variation, this methodology is highly specific and sensitive.The DOAC-Stop procedure eliminated dabigatran from 99.5%, rivaroxaban from 97.9%, and apixaban from 97.1% of participants in our group. Residual amounts did not exceed 2.7 ng/mL for dabigatran, 10.9 ng/mL for rivaroxaban, or 13.03 ng/mL for apixaban, which are safe values that do not affect either screening or special coagulation tests.


Assuntos
Cromatografia Líquida/métodos , Inibidores do Fator Xa/análise , Espectrometria de Massas em Tandem/métodos , Antitrombinas , Coagulação Sanguínea/efeitos dos fármacos , Dabigatrana/análise , Dabigatrana/farmacologia , Dabigatrana/uso terapêutico , Inibidores do Fator Xa/farmacologia , Inibidores do Fator Xa/uso terapêutico , Humanos , Inibidor de Coagulação do Lúpus/sangue , Métodos , Pirazóis/análise , Pirazóis/farmacologia , Pirazóis/uso terapêutico , Piridonas/análise , Piridonas/farmacologia , Piridonas/uso terapêutico , Rivaroxabana/análise , Rivaroxabana/farmacologia , Rivaroxabana/uso terapêutico
20.
Nat Methods ; 16(10): 933, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31562477
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA