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1.
Arch Oral Biol ; 123: 104994, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33472099

RESUMO

OBJECTIVE: To determine the phytochemical composition of Byrsonima gardneriana (A. Juss) leaf extract (BGE) and its antifungal activity against Candida spp., antioxidant potential and in vitro cytotoxicity. MATERIAL AND METHODS: BGE was obtained and submitted to Gas Chromatography Coupled to Mass Spectrometry for phytochemical analysis. The ethanolic extract was tested for its antifungal activity against C. albicans and non-albicans reference strains and clinical isolates in addition to inhibition of C. albicans growth kinetics. It was also tested for antioxidant potential in the presence of phenylhydrazine and reactive oxygen species (ROS). And cytoxicity in human erythrocytes. The data were analyzed by one-way Analysis of Variance (ANOVA) followed by Tukey's or Dunnett's post-hoc test, with α = 0.05. RESULTS: Pyroglutamic acid (90.77 %), eucalyptol (89.61 %) and octanoic acid (76.22 %) were the major compounds detected in BGE, P (%) is the percent probability of compound identification, according to the mass spectra library. The extract showed fungistatic activity, with MIC of 125 µg/mL against most tested strains. While BGE showed low hemolytic activity on all blood types tested herein, it could not prevent osmotic stress in human erythrocytes. The extract did not have oxidizing effects in the presence of phenylhydrazine, but it showed antioxidant potential against ROS when tested at 31 µg/mL and 62 µg/mL. CONCLUSION: B. gardneriana extract showed antifungal activity against Candida spp., demonstrated low hemolytic potential, no oxidant activity in human erythrocytes and antioxidant activity against ROS. This study opens avenues for the study of BGE as a promising biocompatible antifungal agent.


Assuntos
Antifúngicos/farmacologia , Antioxidantes/farmacologia , Malpighiaceae/química , Extratos Vegetais/farmacologia , Antifúngicos/isolamento & purificação , Antioxidantes/isolamento & purificação , Candida/efeitos dos fármacos , Eritrócitos/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Hemólise , Humanos , Testes de Sensibilidade Microbiana , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
2.
Int J Food Microbiol ; 331: 108694, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-32521373

RESUMO

This study evaluated if coatings with chitosan (Chi) and phenolic-rich extract from acerola (Malpighia emarginata D.C., PEA) or jabuticaba (Plinia jaboticaba (Vell.) Berg, PEJ) processing by-products are effective to control the development of rot caused by Lasiodiplodia pseudotheobromae, L. viticola, L. euphorbicola, L. theobromae and L. hormozganensis in papaya (Carica papaya L.) fruit. Effects of formulated coatings on some physicochemical parameters indicative of postharvest quality of papaya were investigated. Twenty-six different phenolics were found in PEA and PEJ, including flavonoids, stilbenes, tannins and phenolic acids. Chi (1-5 mg/mL), PEA and PEJ (25-100 mg/mL) separately caused mycelial growth inhibition on all isolates. Combinations of Chi (3 and 4 mg/mL) and PEA (50 and 75 mg/mL) or PEJ (75 and 100 mg/mL) had additive interactions. Coatings with Chi (4 mg/mL) and PEA (50 or 75 mg/mL) or PEA (75 or 100 mg/mL) inhibited rot development in papaya fruit infected with Lasiodiplodia isolates during 8 days of room temperature storage. Coatings with 4 mg/mL Chi and 75 mg/mL PEA or 100 mg/mL PEJ were the most effective to control rot development. These coatings did not affect negatively physicochemical parameters indicative of postharvest quality of papaya fruit during storage. Coatings with combined Chi and PEA or PEJ could be novel strategies to control postharvest rot caused by Lasiodiplodia in papaya fruit.


Assuntos
Carica/microbiologia , Manipulação de Alimentos/métodos , Microbiologia de Alimentos , Frutas/microbiologia , Malpighiaceae/química , Myrtaceae/química , Extratos Vegetais/farmacologia , Ascomicetos/efeitos dos fármacos , Quitosana/farmacologia , Fenóis/farmacologia
3.
Molecules ; 25(3)2020 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-31991808

RESUMO

Four new diterpenoids, named aspidoptoids A-D (1-4), together with two known analogues (5-6) were isolated from Aspidopterys obcordata vine. Aspidoptoids A-B (1-2) are the first examples of phenylethylene-bearing 20-nor-diterpenoids of which aspidoptoid B (2) possesses a rare 3,10-oxybridge. Their structures and absolute configuration were determined by extensive spectroscopic analyses (IR, HRESIMS, 1D and 2D NMR) and electronic circular dichroism (ECD) calculation. In addition, all the isolates were evaluated for their cytotoxic activities and inhibitory effects on the nitric oxide (NO) production.


Assuntos
Diterpenos/química , Malpighiaceae/química , Extratos Vegetais/química , Animais , Linhagem Celular Tumoral , Diterpenos/farmacologia , Humanos , Espectroscopia de Ressonância Magnética , Camundongos , Modelos Moleculares , Estrutura Molecular , Óxido Nítrico/metabolismo , Extratos Vegetais/farmacologia , Células RAW 264.7
4.
Food Funct ; 11(1): 1037-1048, 2020 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-31819934

RESUMO

Acerola polysaccharides (ACPs) were purified from acerola (Malpighia emarginata DC.), a tropical fruit with strong antioxidant and anti-inflammatory activities. However, the biological activities of ACPs have barely been investigated. The present study was designed to investigate the efficacy of ACPs in the treatment of high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) in C57BL/6 mice. Male C57BL/6 mice were fed with a high-fat diet and treated with different doses of ACPs for 9 continuous weeks. NAFLD was examined in terms of body weight, lipid profiles, liver function markers, and histology. Gene expression was determined by using both qRT-PCR and western blot. Our results showed that administration of ACPs significantly reduced HFD-induced hyperlipidemia and hepatic lipid deposition by inhibiting the SREBP1c pathway in mice. ACP treatment normalized oxidative stress by activating nuclear factor (erythroid-derived-2)-like 2 (Nrf2) and reduced the expressions of pro-inflammatory cytokines in HFD fed mice. Furthermore, ACPs reduced uncoupling protein 2 (UCP2) expression, restored mitochondrial ATP content, increased mitochondrial complex I, IV, and V activity, and increased mitochondrial beta-oxidation by stimulating peroxisomal proliferator-activated receptor-gamma coactivator-1α (PGC-1α) in the liver of HFD-fed mice. Our study indicated that ACPs may be an effective dietary supplement for preventing HFD-induced NAFLD by regulating lipogenesis, reducing inflammation and oxidative stress, and promoting the mitochondrial function.


Assuntos
Lipogênese , Mitocôndrias/metabolismo , Hepatopatia Gordurosa não Alcoólica/terapia , Estresse Oxidativo/efeitos dos fármacos , Polissacarídeos/farmacologia , Animais , Antioxidantes/farmacologia , Dieta Hiperlipídica/efeitos adversos , Masculino , Malpighiaceae/química , Camundongos , Camundongos Endogâmicos C57BL , Hepatopatia Gordurosa não Alcoólica/metabolismo , Oxirredução , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
5.
J Med Food ; 23(2): 173-180, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31502908

RESUMO

Studies involving foods associated with pain reversal and anti-inflammatory effects using zebrafish are rarely reported in the literature. This study aimed to evaluate the effect of graviola (Annona muricata L.) fruit bar (GFB) and GFB added with acerola (Malpighia glabra L) seed extract (ASE) on acute nociception and abdominal inflammation in adult zebrafish (Danio rerio). Acute nociception was induced by formalin, capsaicin, cinnamaldehyde, acidic saline, glutamate (cutaneous models), and hypertonic saline (corneal model), and inflammation was induced by carrageenan. Both GFB and ASE exhibited antinociceptive effect modulated by the nitrergic system, guanylate cyclase, and transient receptor potential ankyrin 1 and acid-sensing ion channels. The antinociceptive effect of GFB also appears to be modulated by the opioid system and glutamatergic receptors (N-methyl-D-aspartate receptor). Only ASE presented corneal antinociceptive effect. Both samples showed anti-inflammatory effect, being more significant the effect of GFB. The addition of acerola by-product extract in GFB results in a product with greater biological potential.


Assuntos
Analgésicos/farmacologia , Annona/química , Anti-Inflamatórios/farmacologia , Inflamação/tratamento farmacológico , Dor Nociceptiva/tratamento farmacológico , Animais , Comportamento Animal , Modelos Animais de Doenças , Feminino , Frutas/química , Masculino , Malpighiaceae/química , Sementes/química , Testes de Toxicidade Aguda , Peixe-Zebra
6.
J Food Sci ; 84(12): 3726-3734, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31834968

RESUMO

Ten probiotic cultures were screened for the ability to hydrolyze soy proteins and bile salt deconjugation (BSD) to select one lactobacilli and one bifidobacteria strain to produce fermented soy beverages (FSBs) containing acerola byproduct (ABP). Next, the effect of the strains and the ABP on the technological and sensory characteristics of these beverages was evaluated during refrigerated storage for up to 28 days. None of the tested strains presented any proteolytic activity against soy proteins. Among the probiotic strains, the best BSD activities were observed for Lactobacillus acidophilus LA-5 and Bifidobacterium longum BB-46, which were further employed, individually or combined, to produce FSB supplemented or not with ABP, using Streptococcus thermophilus TH-4 as a starter, and the effect of these strains and ABP on the technological and sensory acceptability of FSB was evaluated. The probiotic strains did not influence FBS texture parameters, but ABP increased firmness in the ready product. BB-46 increased acidity, therefore decreasing acceptance, whereas the presence of LA-5 and/or ABP increased acceptance, even though the appearance was negatively affected by ABP after 21 days of storage. Thus, the presence of LA-5 and ABP contributed for the sensory acceptance of the FSBs without affecting their technological features. PRACTICAL APPLICATION: Lactobacillus acidophilus LA-5, Bifidobacterium longum BB-46, and/or acerola byproduct (ABP) were applied in the production of fermented soy beverages (FSBs). Principal components analysis was used to evaluate the formulations of the 23 factorial design and the sensory attributes and the effect of storage independently and covariance was the matrix type used for mapping purposes. LA-5 and ABP contributed for the sensory acceptance of FSB, without affecting their technological features, and could be used by food processing companies after scaling up, also reducing the environmental impact by decreasing discarding byproducts, which are sources of bioactive compounds.


Assuntos
Alimentos e Bebidas Fermentados , Lactobacillus/metabolismo , Malpighiaceae/química , Probióticos/metabolismo , Alimentos de Soja , Fermentação/fisiologia , Alimentos e Bebidas Fermentados/análise , Alimentos e Bebidas Fermentados/microbiologia , Manipulação de Alimentos , Alimentos de Soja/análise , Alimentos de Soja/microbiologia
7.
Molecules ; 24(21)2019 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-31684146

RESUMO

This study systematically analyzed the anticancer potential of Acridocarpus orientalis (AO), a traditional medicinal plant of the Arabian Peninsula/East Africa known for its anti-inflammatory and pain relief properties. Tests of serial organic fractions from methanolic extracts of its leaves and stems revealed that only some fractions showed anti-proliferative potential with the dichloromethane fraction from leaves (AOD (L)) showing the most cytotoxic effect against both breast (MCF-7 and MDA-MB-231) and cervical (HeLa) cancer cell lines. The n-butanol fraction from the stems (AOB (S)), on the other hand, was more effective against cervical cancer cells and did not harm the normal cells. Further characterization of the mode of cell killing revealed that AOD (L) depended more on non-apoptotic pathways for its cytotoxicity in breast cancer cells, while it could activate some apoptosis and necroptosis in HeLa cells. The AOB (S) fraction could primarily activate apoptosis and some necroptosis in HeLa cells. Both fractions perturbed autophagy, but in a dissimilar manner. Thus, different parts of A. orientalis revealed variable potential to induce cell death in cancer cells via apoptotic and non-apoptotic pathways, making A. orientalis a valuable plant for the exploration of anticancer bioactive reagents, some of which may be protective for normal cells.


Assuntos
Apoptose/efeitos dos fármacos , Neoplasias da Mama/tratamento farmacológico , Malpighiaceae/química , Neoplasias do Colo do Útero/tratamento farmacológico , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Proliferação de Células/efeitos dos fármacos , Feminino , Células HeLa , Humanos , Células MCF-7 , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Caules de Planta/química
8.
Biomolecules ; 9(11)2019 11 06.
Artigo em Inglês | MEDLINE | ID: mdl-31698710

RESUMO

Murici (Byrsonima crassifolia (L.) Kunth and B. verbascifolia (L.) DC.) and tapereba (Spondias mombin) are Amazonian fruits that contain bioactive compounds. Biochemical and molecular characterization of these fruits can reveal their potential use in preventing diseases, including cancer. The extracts were characterized regarding the presence and profile of carotenoids by high-performance liquid chromatography (HPLC), total phenolic content by the Folin-Ciocalteu assay, and antioxidant activity by antioxidant value 2,2-diphenyl-1-picrylhydrazyl (DPPH) content analysis, 22,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) content analysis, Ferric-Reducing Ability of Plasma (FRAP), and Oxygen Radical Absorbance Capacity (ORAC) analysis. The extracts of tapereba and murici studied were important sources of total carotenoids and lutein, respectively. The extracts were then tested for their effect on the viability of the A2780 ovarian cancer (OC) cell line and its cisplatin (CDDP)-resistant derived cell line, called ACRP, by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Their influence on cell cycle and apoptosis were analyzed by using flow cytometry. Murici and tapereba cell extracts exhibited a strong bioactivity by inhibiting A2780 and ACRP cell viability by 76.37% and 78.37%, respectively, besides modulating the cell cycle and inducing apoptotic cell death. Our results open new perspectives for the development of innovative therapeutic strategies using these Amazon fruit extracts to sensitize ovarian cancer cells to current chemotherapeutic options.


Assuntos
Anacardiaceae/química , Apoptose/efeitos dos fármacos , Frutas/química , Inibidores do Crescimento/farmacologia , Malpighiaceae/química , Neoplasias Ovarianas/fisiopatologia , Extratos Vegetais/farmacologia , Brasil , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Feminino , Inibidores do Crescimento/química , Humanos , Extratos Vegetais/química
9.
Chem Biodivers ; 16(12): e1900442, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31633271

RESUMO

Due to the potentially harmful effects of some synthetic dyes, there is an increasing demand for natural colorants. Recent literature has emphasized the necessity of investigating new sources of dyes. This review discusses the biological sources of dyes derived from the rich plant diversity of Madagascar. As one of the first contributions on the use of these dyestuffs for dyeing textiles, it provides an overview of 128 dye plant species with other potential applications for coloring materials in industry. A detailed description of the botanical and chemical properties of these dyestuffs is given. We believe that the Madagascar plant diversity may be a promising source of novel colorants not yet investigated. We considered it worthwhile to carry out a thorough scientific study of a set of Malagasy plants carefully selected for their coloring properties together with their potential use and valorization in specialized industries where use of natural colorants would be a particular interest.


Assuntos
Corantes/química , Plantas/química , Carotenoides/química , Flavonoides/química , Madagáscar , Malpighiaceae/química , Malpighiaceae/metabolismo , Casca de Planta/química , Casca de Planta/metabolismo , Plantas/metabolismo , Quinonas/química , Taninos/química
10.
Plant Foods Hum Nutr ; 74(3): 358-363, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31209703

RESUMO

The aim of the present study was to identify volatile organic compounds (VOCs) by SPME-GC and quantify the bioactive compounds (ascorbic acid, total flavonoids and total phenolic content), antioxidant capacity (DPPH and ORAC) and physicochemical characteristics of ocorocillo, cambucá, murici da praia and murici do campo, four native South American fruits. A total of 41 volatile compounds were identified in ocorocillo, of which 17 were terpenes. Cambuca's volatile profile contained aldehydes, aromatic hydrocarbons and alcohols. Murici da praia and murici do campo contained high levels of fatty acid volatiles and esters, that contribute to their remarkable aroma. Ocorocillo contained high levels of ascorbic acid and total flavonoids, while cambucá presented lower ascorbic acid, flavonoid and phenolic levels. Murici da praia and murici do campo contained high amounts of phenolic compounds and high free-radical scavenging capacity (DPPH and ORAC). In addition, this fruit was sweeter and less acid compared to the other assessed fruits. The results suggest that these native fruits constitute a good source of volatile compounds and bioactive compounds, which may aid in their preservation interest and potential use in the food, cosmetic and pharmaceutical industries.


Assuntos
Antioxidantes/análise , Eugenia/química , Frutas/química , Malpighiaceae/química , Myrtaceae/química , Compostos Orgânicos Voláteis/análise , Álcoois/química , Fenômenos Químicos , Cromatografia Gasosa , Flavonoides/análise , Depuradores de Radicais Livres/análise , Fenóis/análise , Compostos Fitoquímicos/análise , Microextração em Fase Sólida , Terpenos/análise
11.
Molecules ; 24(10)2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31117291

RESUMO

Obcordata A (OA) is a polyoxypregnane glycoside derived from the Dai medicine Aspidopterys obcordata vines. This study aims to investigate the efficacy of OA on renal tubular epithelial cells exposed to calcium oxalate crystals. We incubated renal tubular cells with 28 µg·cm2 calcium oxalate crystals for 24 h with and without OA, GKT137831, phorbol-12-myristate-13-acetate (PMA), and tocopherol. The MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, microscopic examination, flow cytometry, and immunofluorescence staining revealed that calcium oxalate crystals decreased cell viability and elevated reactive oxygen species (ROS) levels. OA, GKT137831, and tocopherol protected cells and decreased ROS levels. However, OA did not exhibit direct DPPH scavenging ability. In addition, immunoblotting illustrated that OA inhibited the NOX4 (nicotinamide adenine dinucleotide phosphate oxidases 4) expression and downregulated the protein expression in the NOX4/ROS/p38 MAPK (p38 mitogen-activated protein kinase) pathway. The findings suggest that the cytoprotective and antioxidant effects of OA can be blocked by the NOX4 agonist PMA. In conclusion, OA could be used as a NOX4 inhibitor to prevent kidney stones.


Assuntos
Antioxidantes/farmacologia , Túbulos Renais/efeitos dos fármacos , NADPH Oxidase 4/genética , Saponinas/farmacologia , Animais , Antioxidantes/química , Apoptose/efeitos dos fármacos , Compostos de Bifenilo/farmacologia , Oxalato de Cálcio/química , Oxalato de Cálcio/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Humanos , Cálculos Renais/tratamento farmacológico , Cálculos Renais/genética , Cálculos Renais/patologia , Túbulos Renais/patologia , Malpighiaceae/química , Camundongos , Estresse Oxidativo/efeitos dos fármacos , Ésteres de Forbol/farmacologia , Picratos/farmacologia , Pirazóis/farmacologia , Piridinas/farmacologia , Espécies Reativas de Oxigênio/química , Saponinas/química , Tocoferóis/farmacologia , Proteínas Quinases p38 Ativadas por Mitógeno/genética
12.
Food Chem ; 294: 565-571, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31126501

RESUMO

Raman spectroscopy is a rapid and non-destructive analytical technique that has found a growing interest in the characterization and quantification of microconstituents in foods. In this work, Raman spectroscopy was used to evaluate the carotenoids content of processed Bunchosia glandulifera, a native fruit from the Brazilian Atlantic Forest. The B. glandulifera pulps were dried in hot air at 65 and 85 °C, resulting in carotenoids degradation of about 75 and 80%, respectively. The degradation profile of carotenoids over time was analyzed by Raman spectroscopy, with and without the use of an internal standard (TiO2). The reproducibility of the analyses was evaluated by PCA of spectral data. PLS regression was applied for modelling the total carotenoids in B. glandulifera. Resulting models show the Raman data correlate with carotenoids content on samples resulting in a satisfactory coefficient of determination for all sets of samples.


Assuntos
Carotenoides/análise , Malpighiaceae/química , Análise Espectral Raman , Brasil , Dessecação , Frutas/química , Frutas/metabolismo , Análise dos Mínimos Quadrados , Malpighiaceae/metabolismo , Análise de Componente Principal , Reprodutibilidade dos Testes , Temperatura
13.
An Acad Bras Cienc ; 91(1): e20190916, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30994765

RESUMO

Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.


Assuntos
Antifúngicos/química , Antioxidantes/química , Proliferação de Células/efeitos dos fármacos , Malpighiaceae/química , Extratos Vegetais/química , Folhas de Planta/química , Polifenóis/química , Animais , Antifúngicos/farmacologia , Antioxidantes/farmacologia , Candida/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão/métodos , Feminino , Flavonoides/química , Camundongos Endogâmicos BALB C , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia , Polifenóis/farmacologia , Valores de Referência , Baço/citologia , Fatores de Tempo
14.
Meat Sci ; 154: 119-125, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31031210

RESUMO

This research evaluated the effects of different antioxidants on overall quality of beef patties from steers fed distillers grains. Crossbred steers (n = 24) were fed corn, 40% Dry distillers grains plus solubles, or 40% modified distillers grains plus solubles - Dry Matter basis). Patties were made from ground chuck of individual animals and 4 antioxidant treatments were evaluated (CONTROL - no antioxidant, 0.3% ROSEMARY, 0.25% ACEROLA, and 0.6% CITRUS). Diets minimally affected quality attributes. Patties treated with ROSEMARY were shown significant lighter in color than other treatments on day 6. Patties treated with CITRUS were the reddest on days 5 and 6 of display. ACEROLA and CITRUS provided better lipid stability during display when compared to CONTROL and ROSEMARY. Treatment CITRUS reduced APC growth by 2 log when compared to all other treatments (P < 0.01). Although patties treated with CITRUS had higher off-flavor intensity, no effect was observed for overall desirability. Patties treated with 0.6% of CITRUS provided optimal overall quality.


Assuntos
Ração Animal/análise , Antioxidantes , Conservação de Alimentos/métodos , Produtos da Carne/análise , Adulto , Animais , Bovinos , Citrus/química , Dieta/veterinária , Grão Comestível , Feminino , Humanos , Masculino , Malpighiaceae/química , Produtos da Carne/microbiologia , Pessoa de Meia-Idade , Extratos Vegetais/química , Rosmarinus/química , Paladar
15.
Biomed Pharmacother ; 111: 1112-1123, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30841424

RESUMO

Byrsonima intermedia is a species of bush popularly used to treat gastrointestinal disorders, such as gastric ulcers, gastritis, and diarrhea. Previous studies have revealed that the methanolic crude extract of B. intermedia leaves has gastroprotective and healing properties. In this new study, we specifically investigated two purified partitions, ethyl acetate (EtOAc) and water (AcoAq), obtained from the crude extract to characterize the antiulcer effects of these two partitions and the mechanisms of action of this medicinal plant. The healing effects of these partitions on the gastric and duodenal mucosa were assessed after ischemia-reperfusion (I/R) or acetic acid-induced injury. The involvement of tumor necrosis factor-alpha (TNF-alpha), interleukin 1ß (IL-1ß), interleukin 10 (IL-10), and myeloperoxidase (MPO) activity and glutathione (GSH) levels were determined. The antibacterial activity against Helicobacter pylori was evaluated using microdilution methods. The phytochemical analysis of AcoAq revealed a predominance of oligomeric proanthocyanidins and galloyl quinic esters, whereas EtOAc was found to contain concentrated flavonoids. Both partitions led to a significant reduction in gastric lesions, but AcoAq was more effective than EtOAc with regard to anti-Helicobacter pylori activity in addition to protecting the gastric mucosa against ethanol, non-steroidal anti-inflammatory drugs (NSAIDs) and duodenal mucosal damage induced by cysteamine. Additionally, both partitions were associated with a significant increase in gastric and duodenal healing and increased gastric mucosal GSH content after damage induced by acetic acid. On the other hand, after 6 days of treatment, EtOAc was more effective than AcoAq in ameliorating gastric damage upon initiation of the gastric I/R, which was accompanied by a significant reduction in the activity of gastric mucosal MPO, IL 1-ß and TNF-alpha, as well as an elevation in IL-10 and GSH content. These results demonstrate that the oligomeric proanthocyanidins and galloyl quinic esters present in AcoAq were more effective in the prevention of gastric and duodenal ulcers due to the antioxidant effects of these compounds, whereas the flavonoids present in EtOAc were more effective due to their anti-inflammatory activity on the gastric and duodenal tissue. All these results confirm that the rich phytochemical diversity of B. intermedia contributes to the pharmacological actions of this medicinal plant on the gastrointestinal tract in addition to its activity against H. pylori.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Malpighiaceae/química , Úlcera Péptica/tratamento farmacológico , Animais , Antiulcerosos/farmacologia , Flavonoides/farmacologia , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , Gastrite/tratamento farmacológico , Gastrite/metabolismo , Glutationa/metabolismo , Masculino , Medicina Tradicional/métodos , Úlcera Péptica/metabolismo , Compostos Fitoquímicos/farmacologia , Fitoterapia/métodos , Extratos Vegetais/farmacologia , Folhas de Planta/química , Plantas Medicinais/química , Ratos , Ratos Wistar , Estômago/efeitos dos fármacos , Cicatrização/efeitos dos fármacos
16.
Food Res Int ; 115: 16-22, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30599927

RESUMO

This work examines the influence of glow plasma technology on vitamins, carotenoids, phenolic content, antioxidant capacity, and color of acerola juice. The effects of nitrogen plasma treatment were observed at different operation conditions: flowrates (from 10 to 20 mL/min) and processing times (5 to 15 min). The study explored the suitability of glow plasma on 40 mL samples, which is considered a large sample of current plasma technology studies. Nitrogen glow plasma increased the content of vitamin A and carotenoids in acerola juice under specific processing conditions. The highest increase of vitamin A and carotenoids was found at gas flow rate of 10 mL/min and 10 min of treatment time. The effect of glow plasma on vitamin C (main vitamin in acerola juice) was not significant, maintaining the retention of vitamin C above 95% under all operating conditions that were studied. The phenolics content reduced by 30% due to reaction with reactive nitrogen species when the juice was treated at the most severe condition (gas flow rate of 20 mL/min and 15 min of treatment time). The antioxidant capacity was little affected by the reactive nitrogen species formed by N2 glow plasma, which remained relatively constant at high plasma flow rate (20 mL/min) but reduced when lower gas flow rates were used (10 mL/min). The typical orange color of the juice improved due to the increase in free carotenoid content. In summary, this study showed that the operating conditions of glow plasma treatment could be adjusted to have a positive influence on vitamin A, carotenoids and color of acerola juice.


Assuntos
Manipulação de Alimentos , Sucos de Frutas e Vegetais/análise , Malpighiaceae/química , Antioxidantes/análise , Ácido Ascórbico/análise , Carotenoides/análise , Cor , Análise de Alimentos , Frutas/química , Valor Nutritivo , Fenóis/análise , Vitamina A/análise
17.
Food Chem ; 277: 698-705, 2019 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-30502205

RESUMO

In this study, we compared the antioxidant activity of ripe and unripe acerola extracts with synthetic antioxidants (BHA and BHT). This activity was assessed by classical approaches (DPPH and ABTS) and by an in vivo method using yeasts. Acerola extracts contain phenolic compounds and ascorbic acid that exhibit radical scavenger capacity and reducing power. The results obtained with yeasts revealed that the acerola extracts and BHT either acted as antioxidants or presented no activity depending on the nature of the oxidant molecule used. BHA decreased yeast resistance to oxidative treatments and also showed deleterious effects even when oxidative treatments were not applied. The unripe acerola was the most efficient antioxidant in the in vitro experiments but not necessarily in the in vivo assays, showing the weakness of in vitro systems in predicting antioxidant responses for biological purposes. BHA presented cell damaging effects even in the absence of oxidizing reagents.


Assuntos
Antioxidantes/química , Malpighiaceae/química , Extratos Vegetais/química , Saccharomyces cerevisiae/metabolismo , Antioxidantes/síntese química , Antioxidantes/farmacologia , Ácido Ascórbico/química , Ácido Ascórbico/isolamento & purificação , Ácido Ascórbico/farmacologia , Peróxido de Hidrogênio/farmacologia , Malpighiaceae/metabolismo , Fenóis/química , Fenóis/isolamento & purificação , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Saccharomyces cerevisiae/crescimento & desenvolvimento , Vitamina K 3/farmacologia
18.
Toxicon ; 158: 47-50, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30471379

RESUMO

Toxic plants containing monofluoroacetate (MFA) cause sudden death in livestock in Australia, South Africa and Brazil, causing economic losses to producers. The objective of this study was to determine the amount of MFA present in young leaves, mature leaves, senescent leaves, and seeds of Amorimia pubiflora harvested at different times of the year and to determine their toxic effect on sheep. Samples of Amorimia pubiflora were collected during April, August and December of 2015 and March of 2016, separated according to the vegetative stage (young leaves, seeds, mature leaves, and senescent leaves), dried in an oven, and administered in daily doses of 5 g/kg/body weight (bw) of fresh leaves to sheep through ruminal cannulae. The experiment was divided into four stages according to the time of collection of the plant so that each sheep received a different vegetative stage of the plant (young leaves, mature leaves, and senescent leaves). Only in the second stage of the experiment was it possible to collect A. pubiflora seeds, which were administered using the same method used for the administration of the leaves. The sheep were dosed with the plant until they showed clinical signs of toxicosis or until the plant was no longer available. Aliquots of leaves and seeds of A. pubiflora were analyzed for MFA concentration. The seeds and young leaves had higher concentrations of MFA than did the mature (harvested in August and December) and senescent (harvested in December) leaves. However, all vegetative stages of the plant were toxic and caused fatal poisoning. The results of our study showed that A. pubiflora is toxic to sheep even when MFA concentrations are low, demonstrating that the presence of this substance is a risk factor for the occurrence of poisoning. Knowing the toxic principle and its variations allow us to determine the conditions for the occurrence of plant toxicosis as well as possible treatment, control, and prophylaxis methods, contributing significantly to the reduction of economic losses on farms due to plant poisoning.


Assuntos
Fluoracetatos/análise , Malpighiaceae/toxicidade , Plantas Tóxicas/química , Animais , Brasil , Malpighiaceae/química , Folhas de Planta/química , Folhas de Planta/toxicidade , Intoxicação por Plantas/veterinária , Plantas Tóxicas/toxicidade , Sementes/química , Sementes/toxicidade , Ovinos , Doenças dos Ovinos/induzido quimicamente , Carneiro Doméstico
19.
Int J Food Sci Nutr ; 70(2): 182-194, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30160546

RESUMO

In this study, we first investigated the survival of three probiotic strains, individually and combined with acerola by-product during simulated gastrointestinal conditions. Next, we investigated the effects of acerola by-product combined with Bifidobacterium longum BB-46 on a gut microbiota model (SHIME®). Chemical composition, total phenolic compounds, antioxidant activity of the acerola by-product and microbial counts, denaturing gradient gel electrophoresis (DGGE), ammonium ions ( NH4+ ) and short-chain fatty acids (SCFAs) analysis of the SHIME® samples were performed. Acerola by-product revealed high protein and fibre, reduced lipid contents, and showed to be an excellent source of total phenolic compounds with high in vitro antioxidant activity. A decreased amount of NH4+ in the ascending colon and an increase (p < .05) in SCFAs were observed in the three regions of colon during treatment with BB-46 and acerola by-product. BB-46 combined with acerola by-product showed positive effects on the gut microbiota metabolism in SHIME® model.


Assuntos
Antioxidantes/farmacologia , Bifidobacterium longum , Colo/metabolismo , Microbioma Gastrointestinal , Malpighiaceae/química , Fenóis/farmacologia , Probióticos , Compostos de Amônio/metabolismo , Antioxidantes/análise , Bifidobacterium longum/crescimento & desenvolvimento , Bifidobacterium longum/metabolismo , Colo/efeitos dos fármacos , Gorduras na Dieta/análise , Fibras na Dieta/análise , Proteínas na Dieta/análise , Frutas/química , Humanos , Valor Nutritivo , Fenóis/análise , Preparações de Plantas/química , Preparações de Plantas/farmacologia
20.
J Sci Food Agric ; 99(6): 2855-2864, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30450558

RESUMO

BACKGROUND: Fruits present high concentrations of bioactive compounds that are beneficial to health due to their antioxidant properties. New alternatives to vegetable oils with such properties have been studied. We determined the chemical compounds of Byrsonima cydoniifolia A. Juss. fruits in three ripening stages as well as the optical behavior and quality level of the oils. RESULTS: The ripening stage affected the chemical composition of the fruits and oils. The fruits presented high values of bioactive compounds, as ascorbic acid (1.46-1.82 g kg-1 ) and total phenols (3.54-15.91 g gallic acid equivalents kg-1 ), as well as showed excellent antioxidant activity. The ripe fruits showed high content of carotenoids (45.90 mg kg-1 ) were lutein is the major carotenoid, representing 55.56%. The oil of the ripe fruits showed high lipid content (252.6 g kg-1 ) and contained oleic (521.83 g kg-1 ), palmitic (209.13 g kg-1 ) and linoleic (195.4 g kg-1 ) fatty acids. The oil of ripe fruits showed the best oxidative stability, with longer induction period (22.29 h) than the other oils. CONCLUSION: Fruits of B. cydoniifolia A. Juss. demonstrate to possess a promising potential for the use as functional ingredients. The oil of the ripe fruits was more stable and presents a greater potential to be used for edible purposes. © 2018 Society of Chemical Industry.


Assuntos
Carotenoides/química , Frutas/crescimento & desenvolvimento , Malpighiaceae/química , Óleos Vegetais/química , Antioxidantes/química , Ácidos Graxos/química , Frutas/química , Malpighiaceae/crescimento & desenvolvimento , Oxirredução
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