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1.
Biochem Med (Zagreb) ; 30(1): 010502, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31839720

RESUMO

Extravascular body fluids (EBF) analysis can provide useful information in the differential diagnosis of conditions that caused their accumulation. Their unique nature and particular requirements accompanying EBF analysis need to be recognized in order to minimize possible negative implications on patient safety. This recommendation was prepared by the members of the Working group for extravascular body fluid samples (WG EBFS). It is designed to address the total testing process and clinical significance of tests used in EBF analysis. The recommendation begins with a chapter addressing validation of methods used in EBF analysis, and continues with specific recommendations for serous fluids analysis. It is organized in sections referring to the preanalytical, analytical and postanalytical phase with specific recommendations presented in boxes. Its main goal is to assist in the attainment of national harmonization of serous fluid analysis and ultimately improve patient safety and healthcare outcomes. This recommendation is intended to all laboratory professionals performing EBF analysis and healthcare professionals involved in EBF collection and processing. Cytological and microbiological evaluations of EBF are beyond the scope of this document.


Assuntos
Líquidos Corporais/química , Técnicas de Laboratório Clínico/normas , Líquidos Corporais/metabolismo , Exsudatos e Transudatos/química , Exsudatos e Transudatos/metabolismo , Guias como Assunto , Humanos , Segurança do Paciente , Derrame Pleural/diagnóstico , Garantia da Qualidade dos Cuidados de Saúde , Sociedades Médicas , Manejo de Espécimes/normas
2.
Sud Med Ekspert ; 62(5): 39-42, 2019.
Artigo em Russo | MEDLINE | ID: mdl-31626193

RESUMO

In medical literature there is no single detailed standard for studying the biochemical composition of a tooth. This circumstance led to the development of a proprietary method for determining the enantiomers of aspartic acid in hard dental tissues for use in the further study of the relationship between the amino acid composition of dental tissues and the biological age of humans. Dental tissue samples obtained from living individuals aged from 20 to 50 years were studied by chromatography-mass spectrometry. The results of the study allowed us to put forward a number of practical recommendations on the standardization of the procedure for the preparation of dental tissue samples and the parameters of chromatography-mass spectrometry research.


Assuntos
Determinação da Idade pelos Dentes , Ácido Aspártico/análise , Adulto , Cromatografia , Odontologia Legal , Dureza , Humanos , Espectrometria de Massas , Pessoa de Meia-Idade , Manejo de Espécimes/normas , Adulto Jovem
3.
Hum Genet ; 138(11-12): 1287-1299, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31620872

RESUMO

Biobank operations started officially in Finland in 2013 when the Biobank Act defining and regulating biobank operations came into force. Since then, ten biobanks have been established and they have started to collect new prospective samples with broad consent. The main corpus of biobank samples, however, consists of approximately 10 million "legacy samples". These are old diagnostic or research samples that were transferred to biobanks in accordance with the Biobank Act. The focus of this article is on ambiguities concerning these legacy samples and their transfer in terms of legality, human rights, autonomy, and social sustainability. We analyse the Finnish biobank operations in the context of international regulation, such as the European Convention of Human Rights, the Oviedo Convention, European Charter of Fundamental Rights, the GDPR, and EU Clinical Trials Regulation, and show that the practice of using legacy samples is at times problematic in relation to this regulatory framework. We argue that the prevailing interpretations of these regulations as translated into the Finnish biobank practices undermine the autonomy of individuals by not giving individuals a right to consent or an actionable right to opt-out of the transfer of these legacy samples to the biobank. This is due to the fact that individuals are not given effective notification of such transfers. Thus, issues regarding the legal status of the biobank samples and the social sustainability of biobank operations remain a challenge for biobanks in Finland despite governmental efforts to create pioneering, comprehensive, and enabling legislation.


Assuntos
Bancos de Espécimes Biológicos/legislação & jurisprudência , Bancos de Espécimes Biológicos/normas , Pesquisa Biomédica/ética , Manejo de Espécimes/ética , Manejo de Espécimes/normas , Finlândia , Humanos , Estudos Prospectivos
4.
Am J Vet Res ; 80(8): 787-791, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31339768

RESUMO

OBJECTIVE: To evaluate safety of stylet-in and stylet-out techniques for collection of CSF from the cisterna magna and to assess whether there were differences between techniques with regard to contamination of samples, sample quality, and efficiency of collection. ANIMALS: 10 adult purpose-bred research Beagles. PROCEDURES: A prospective crossover study was conducted. Preanesthetic physical and neurologic examinations and hematologic analyses were performed. Dogs were anesthetized, and collection of CSF samples from the cisterna magna by use of a stylet-in or stylet-out technique was performed. Two weeks later, samples were collected with the other sample collection technique. Samples of CSF were processed within 1 hour after collection. RESULTS: Cellular debris was detected in higher numbers in stylet-in samples, although this did not affect sample quality. The stylet-out technique was performed more rapidly. No adverse effects were detected for either technique. CONCLUSIONS AND CLINICAL RELEVANCE: Both techniques could be safely performed in healthy anesthetized dogs. The stylet-out technique was performed more rapidly and yielded a sample with less cellular debris. Both techniques can be used in clinical practice to yield CSF samples with good diagnostic quality.


Assuntos
Líquido Cefalorraquidiano , Cisterna Magna , Cães/líquido cefalorraquidiano , Manejo de Espécimes/veterinária , Punção Espinal/veterinária , Animais , Cisterna Magna/cirurgia , Estudos Cross-Over , Feminino , Masculino , Agulhas , Estudos Prospectivos , Manejo de Espécimes/instrumentação , Manejo de Espécimes/métodos , Manejo de Espécimes/normas , Punção Espinal/instrumentação , Punção Espinal/métodos , Punção Espinal/normas
5.
Theriogenology ; 137: 88-92, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31186129

RESUMO

Reducing the number of spermatozoa per artificial insemination (AI) dose and managing semen in ways to ensure greater quality at the same time represents current challenges with sperm processing in pig AI centers. Based on a multi-year comparative analysis of process steps in different pig AI centers, and complementary experimental studies under standardized laboratory conditions, current process standards for the preservation of boar semen have been updated and new ones developed. Currently, these standards represent an integral part of the quality assurance of 29 European pig AI centers in ten different organizations in Germany, Switzerland and Austria. Improvement of hygiene management and guidelines for prudent use of antibiotics have become key issues. Furthermore, new quality control tools have been implemented in the processing and transport of boar semen: e.g. refractometry as an easy-to-use tool to estimate extender osmolarity and 'mobile sensing' apps for continuous monitoring of various environmental parameters. Moreover, based on a series of experiments under laboratory and field conditions, guidelines for optimizing the dilution process, and time and temperature management during boar semen processing, have been developed and implemented. Similarly, recommendations for the handling of semen doses during storage have been renewed. Over the years, the efficiency of the quality assurance system has been reflected by a decrease of bacterial contamination and a concomitant increase in the quality of semen doses. In conclusion, science-based quality assurance is an effective way to improve the production performance in pig AI centers, resulting in high quality and economically-priced semen for pig producers. Increasing knowledge of sperm physiology together with computational and technical innovations will continue to develop and modify quality assurance concepts in the future.


Assuntos
Abrigo para Animais/normas , Inseminação Artificial/veterinária , Análise do Sêmen/veterinária , Suínos , Animais , Europa (Continente) , Masculino , Manejo de Espécimes/métodos , Manejo de Espécimes/normas , Manejo de Espécimes/veterinária , Suínos/microbiologia
6.
Int J Lab Hematol ; 41 Suppl 1: 162-169, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31069971

RESUMO

In the coagulation laboratory, much emphasis has been placed on rapid and accurate testing; however, spurious results that are inaccurate and do not reflect the actual status of the patient can potentially lead to an incorrect diagnosis and altered intervention. Errors in coagulation results and interpretation can occur at any point of the process from obtaining the specimen to interpretation and use of the result by the clinician. The main sources of error include the patient's biological and preanalytical variation, analytical testing, and postanalytical use of the reported result(s). This article reviews various sources of error leading to spurious results, providing methods to recognize these aberrant results and presenting solutions for minimizing their occurrence.


Assuntos
Testes de Coagulação Sanguínea , Erros de Diagnóstico/prevenção & controle , Manejo de Espécimes , Humanos , Manejo de Espécimes/métodos , Manejo de Espécimes/normas
7.
Int J Lab Hematol ; 41 Suppl 1: 49-55, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31069983

RESUMO

Coagulation laboratory external quality assurance (EQA) programs provide information that satisfies regulatory requirements and improves laboratory quality, patient care, and safety. In addition to the value to individual laboratories, data from EQA programs benefits the laboratory community in multiple ways by providing a snapshot of laboratory practice and summarizing the performance of various methods in identifying normal and abnormal specimens and the effects of therapies or interfering substances. This review article aims to summarize and provide examples of some of the important lessons learned from coagulation EQA data, including issues related to non-standardization, imprecision, and the effects of interfering substances.


Assuntos
Coagulação Sanguínea , Garantia da Qualidade dos Cuidados de Saúde , Controle de Qualidade , Manejo de Espécimes , Testes de Coagulação Sanguínea/métodos , Testes de Coagulação Sanguínea/normas , Humanos , Manejo de Espécimes/métodos , Manejo de Espécimes/normas
8.
Ann Biol Clin (Paris) ; 77(2): 131-154, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30998194

RESUMO

This document provides a joint recommendation for venous blood sampling of the European federation of clinical chemistry and laboratory medicine (EFLM) Working Group for preanalytical phase (WG-PRE) and Latin American working group for preanalytical phase (WG-PRE-LATAM) of the Latin America confederation of clinical biochemistry (COLABIOCLI). It offers guidance on the requirements for ensuring that blood collection is a safe and patient-centered procedure and provides practical guidance on how to successfully overcome potential barriers and obstacles to its widespread implementation. The target audience for this recommendation are healthcare staff members directly involved in blood collection. This recommendation applies to the use of a closed blood collection system and does not provide guidance for the blood collection with an open needle and syringe and catheter collections. Moreover, this document neither addresses patient consent, test ordering, sample handling and transport nor collection from children and unconscious patients. The recommended procedure is based on the best available evidence. Each step was graded using a system that scores the quality of the evidence and the strength of the recommendation. The process of grading was done at several face-to-face meetings involving the same mixture of stakeholders stated previously. The main parts of this recommendation are: 1) Pre-sampling procedures, 2) Sampling procedure, 3) Post-sampling procedures and 4) Implementation. A first draft of the recommendation was circulated to EFLM members for public consultation. WG-PRE-LATAM was also invited to comment the document. A revised version has been sent for voting on to all EFLM and COLABIOCLI members and has been officially endorsed by 33/40 EFLM and 21/21 COLABIOCLI members. We encourage professionals throughout Europe and Latin America to adopt and implement this recommendation to improve the quality of blood collection practices and increase patient and workers safety.


Assuntos
Coleta de Amostras Sanguíneas/normas , Química Clínica/normas , Técnicas de Laboratório Clínico/normas , Flebotomia/normas , Fase Pré-Analítica/normas , Adulto , Coleta de Amostras Sanguíneas/métodos , Química Clínica/organização & administração , Criança , Técnicas de Laboratório Clínico/métodos , Europa (Continente) , Humanos , América Latina , Flebotomia/métodos , Fase Pré-Analítica/métodos , Sociedades Médicas/organização & administração , Sociedades Médicas/normas , Manejo de Espécimes/métodos , Manejo de Espécimes/normas
9.
Ann Biol Clin (Paris) ; 77(2): 225-228, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30998201

RESUMO

The culture of micro-organisms exposes to the risk of microbiological contamination at all stages of the analysis: inoculation on culture media, incubation, and observation of cultures. During our accreditation renewal audit, a surveillance point was notified, regarding the lack of consideration of the risk of microbiological contamination. Its mastery mainly relies on cleaning/disinfection operations and their traceability. In addition, several strategies based on environmental sampling or indicators can be performed. We propose a risk analysis in order to present these strategies.


Assuntos
Poluição Ambiental/análise , Contaminação de Equipamentos , Estudos de Avaliação como Assunto , Laboratórios/normas , Microbiologia/normas , Meios de Cultura/análise , Desinfecção , Microbiologia Ambiental , Fungos/crescimento & desenvolvimento , Humanos , Técnicas Microbiológicas , Controle de Qualidade , Manejo de Espécimes/métodos , Manejo de Espécimes/normas
10.
J Fish Biol ; 95(2): 647-650, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30963579

RESUMO

Cross-contamination of epidermal mucus was assessed at three sampling locations on the bodies of Pacific halibut Hippoglossus stenolepis by inducing contact between fish coated with labelled synthetic mucus and non-treated fish. Results indicate a positive relationship between sampling site exposure and sample contamination and that mucous sample cross-contamination can be mitigated by sampling in a location protected from external contact.


Assuntos
Epiderme/química , Linguado/fisiologia , Muco/química , Manejo de Espécimes/veterinária , Alaska , Nadadeiras de Animais/química , Animais , Congelamento , Sistema da Linha Lateral/química , Manejo de Espécimes/métodos , Manejo de Espécimes/normas
11.
Rev. esp. quimioter ; 32(2): 130-136, abr. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-182814

RESUMO

Introduction: Evaluate the efficacy of an information system addressed to nursing staff to lower the blood culture contamination rate. Methods: A blind clinical trial was conducted at Internal Medicine and Emergency Departments during 2011. After following a reeducation program in BC extraction, participants were randomly selected in a 1:1 ratio. Every participant of the experimental group was informed of each worker's individual performance; whereas the control group was only informed of the global results. Results: A total of 977 blood extractions were performed in 12 months. Blood culture contamination rate was 7.5%. This rate was higher in the Emergency Department than in Internal Medicine (10% vs. 3.8%; p=0.001). Factors associated with the higher risk of contamination were, in the univariate analysis, the extraction through a recently implanted blood route and the time of professional experience, while those associated with a lower risk were the extraction in Internal Medicine and through a butterfly needle. On multivariate analysis, extraction through a recently placed access was an independent risk factor for an increased contamination rate (OR 2.29; 95%CI 1.18-4.44, p=0.014), while individual information about the blood culture results (OR 0.11; 95%CI 0.023-0.57; p=0.008), and more than 9 years of professional experience were asso-ciated with fewer contaminations (OR 0.30; 95%CI 0.12-0.77; p=0.012). In the intervention group the contamination rate diminished by a 26 %. Conclusions: Drawing blood cultures through a recently taken peripheral venous access increased their risk of contamination. The intervention informing the nurse staff of the contamination rate is effective to decrease it


Objetivos: Evaluar la eficacia de un sistema de información dirigido al personal de enfermería, en la reducción de la tasa de contaminación de los hemocultivos. Métodos: Durante el año 2011, se realizó un ensayo clínico en los servicios de Medicina Interna y de Urgencias. Después de seguir un programa de reeducación en la extracción de los hemocultivos, los participantes, fueron aleatorizados en una proporción de 1:1. En el grupo de intervención se informó del porcentaje de hemocultivos contaminados de cada profesional y en el grupo control se aportaba la información del porcentaje global de contaminaciones. Resultados: Durante un periodo de 12 meses se realizaron 977 extracciones. La tasa de contaminación de los hemocultivos fue del 7,5%. Esta tasa fue mayor en Urgencias que en Medicina Interna (10% versus 3,8%, p=0,001). Los factores asociados con mayor riesgo de contaminación fueron, en el análisis univariable: la extracción a través de una vía sanguínea recientemente implantada y el tiempo de experiencia profesional; mientras que los que se asociaron con menor riesgo fueron la extracción en Medicina Interna (versus en Urgencias) y a través de una palomilla. En el análisis multivariable, la extracción de los hemocultivos de una vía recientemente implantada se relacionó de forma independiente con un incremento de las contaminaciones (OR 2,29, IC 95% 1,18-4,44, p=0,014),mientras que la información individual sobre los resultados de los hemocultivos (OR 0.11; IC 95% 0,023-0,57; p=0,008) y la experiencia profesional mayor de 9 años, lo hizo con menos contaminaciones (OR 0,30, IC 95% 0,12-0,77, p=0,012). En el grupo de intervención la tasa de contaminaciones se redujo en un 26%. Conclusión: La extracción de hemocultivos a través de una vena periférica recientemente implantada aumentó el riesgo de contaminación de los mismos. La intervención informativa a los enfermeros de la tasa de contaminación de los hemocultivos, es eficaz para disminuirla


Assuntos
Humanos , Manejo de Espécimes/normas , Contaminação Biológica/prevenção & controle , Preservação de Amostras/métodos , Hemocultura/normas , Processo de Enfermagem/normas , Coleta de Amostras Sanguíneas/normas , Métodos Analíticos de Preparação de Amostras/normas , Contaminação de Equipamentos/prevenção & controle
12.
Influenza Other Respir Viruses ; 13(4): 319-330, 2019 07.
Artigo em Inglês | MEDLINE | ID: mdl-30925029

RESUMO

BACKGROUND: Internet-based participatory surveillance systems, such as the German GrippeWeb, monitor the frequency of acute respiratory illnesses on population level. In order to interpret syndromic information better, we devised a microbiological feasibility study (GrippeWeb-Plus) to test whether self-collection of anterior nasal swabs is operationally possible, acceptable for participants and can yield valid data. METHODS: We recruited 103 GrippeWeb participants (73 adults and 30 children) and provided them with a kit, instructions and a questionnaire for each sample. In the first half of 2016, participants took an anterior nasal swab and sent it to the Robert Koch Institute whenever an acute respiratory illness occurred. Reporting of illnesses through the GrippeWeb platform continued as usual. We analysed swabs for the presence of human c-myc-DNA and 22 viral and bacterial pathogens. After the study, we sent participants an evaluation questionnaire. We analysed timeliness, completeness, acceptability and validity. RESULTS: One hundred and two participants submitted 225 analysable swabs. Ninety per cent of swabs were taken within 3 days of symptom onset. Eighty-nine per cent of swabs had a corresponding reported illness in the GrippeWeb system. Ninety-nine per cent of adults and 96% of children would be willing to participate in a self-swabbing scheme for a longer period. All swabs contained c-myc-DNA. In 119 swabs, we identified any of 14 viruses but no bacteria. The positivity rate of influenza was similar to that in the German physician sentinel. CONCLUSION: Self-collection of anterior nasal swabs proofed to be feasible, was well accepted by participants, gave valid results and was an informative adjunct to syndromic data.


Assuntos
Monitoramento Epidemiológico , Nariz/virologia , Infecções Respiratórias/diagnóstico , Infecções Respiratórias/virologia , Manejo de Espécimes/métodos , Vírus/isolamento & purificação , Doença Aguda/epidemiologia , Adulto , Criança , Estudos de Viabilidade , Alemanha , Humanos , Influenza Humana/diagnóstico , Manejo de Espécimes/normas , Inquéritos e Questionários , Vírus/genética
13.
BMC Res Notes ; 12(1): 165, 2019 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-30904022

RESUMO

OBJECTIVE: With the democratization of genetic testing, researchers, clinicians, and educators must consider the varying degree of field conditions when collecting samples for genetic analyses. For genotyping or sequencing studies, study designers have multiple options from which to choose, including cheek swabs and saliva sampling. One significant benefit of saliva collection is that it can be done remotely, in the privacy of one's home. This same benefit adds a risk of compliance. Therefore, our goal with this study was to see if the quality and quantity of the saliva collection by a saliva DNA collection kit would be affected by not following the manufacturer's directions, i.e., drinking or eating right before collection. RESULTS: We asked five participants to collect saliva samples according to the manufacturer's guidance and also after consuming five food items or beverages. We evaluated DNA quantity and quality post-purification using spectroscopy, electrophoresis, and polymerase chain reaction genotyping. Consistent with our hypothesis, we did not see a difference in quantity or quality of the isolated DNA. From our results, we conclude that the manufacturer's instructions serve as an ideal guideline, but the collection devices are robust enough to permit flexibility in sampling at home or in the field.


Assuntos
DNA/análise , Saliva/química , Manejo de Espécimes/normas , Adulto , Bebidas , Alimentos , Humanos
14.
Biopreserv Biobank ; 17(2): 139-142, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30912671

RESUMO

Adequate preservation of biospecimens has been proven to be critical to obtain reliable and reproducible results in genomics, transcriptomics, proteomics, and many other assays. Most biological assays can be performed on specimens preserved in -80°C ultra-low freezers, but their quality can be influenced by temperature variability within storage units. Thus, regulatory standards such as those from the College of American Pathologists (CAP), the federal Clinical Laboratory Improvement Amendments, and standards from the Food and Drug Administration require temperature mapping, a standard quality assessment for accreditation when using ultra-low freezers for long-term biospecimen storage. The current mapping methods, providing annual/periodic data, may not be adequate indicators of temperature stability within the different zones of the freezers. In addition, they frequently require manual handling of biospecimens periodically, as they require freezers to be emptied or rearranged temporarily for the installation of temperature probes, risking the integrity of biospecimen quality. In this article, we describe a novel monitoring methodology based on real-time temperature reading of multiple zones by permanently installing thermocouples. An online cloud-based application records temperature variations within 1 minute intervals, and its 24/7 alert system triggers text alarm messages to a predefined set of users when temperature values increase above preset defaults. This provides an opportunity to take remedial action and to obtain a better-quality assessment. Our results indicate that real-time temperature monitoring at multiple zones of a freezer with a 1 minute resolution is a stable and sustainable methodology and, most importantly, lowers the risk of compromising the quality of the biospecimen. The design and use of the real-time monitoring system for ultra-low freezers is one of the acceptable methods by CAP to ensure the stability of biospecimen quality during long-term storage.


Assuntos
Criopreservação/instrumentação , Criopreservação/normas , Controle de Qualidade , Manejo de Espécimes/instrumentação , Manejo de Espécimes/normas , Termografia , Criopreservação/métodos , Manejo de Espécimes/métodos
15.
Rev Bras Enferm ; 72(1): 118-124, 2019.
Artigo em Inglês, Português | MEDLINE | ID: mdl-30916276

RESUMO

OBJECTIVE: To analyze women's perception of the quality of the service provided for colposcopy test in the city of Belém (PA), Brazil. METHOD: Quantitative-descriptive study on 400 women who underwent Pap test on the public healthcare network, using the Service Quality (SERVQUAL) model. We used the Cronbach's alpha index to measure the reliability of the scale, and data were analyzed by quartiles of the gaps of the dimensions: tangibility, responsiveness, reliability, assurance, and empathy. RESULTS: The assurance dimension, corresponding to the knowledge and courtesy of staff, featured the highest degree of importance to users; and empathy, which corresponds to the staff's concern with women's needs, featured the lower degree. The most expressive negative gaps concerned the structure of services and the attitude of healthcare professionals when collecting the material. CONCLUSION: All gaps featured negative relationship between what was expected and perceived, expressing dissatisfaction regarding the service.


Assuntos
Colonoscopia/normas , Teste de Papanicolaou/métodos , Satisfação do Paciente , Percepção , Manejo de Espécimes/normas , Adulto , Idoso , Brasil , Colonoscopia/psicologia , Feminino , Humanos , Pessoa de Meia-Idade , Teste de Papanicolaou/normas , Qualidade da Assistência à Saúde/normas , Manejo de Espécimes/psicologia , Inquéritos e Questionários
16.
BMC Res Notes ; 12(1): 159, 2019 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-30902062

RESUMO

OBJECTIVE: Stabilising samples of microbial communities for DNA extraction without access to laboratory equipment can be a challenging task. In this paper we propose a method using filter paper disks for the preservation of DNA from diverse microbial communities which are found in a fermented milk product. RESULTS: Small adaptations to the DNA extraction method used for liquid fermented milk delivered DNA of sufficient amounts and quality to be used for later analyses, e.g. full community 16S amplicon sequencing. The microbial community structure obtained via the filter paper method showed sufficient resemblance to the structure obtain via the traditional DNA extraction from the liquid milk sample. This method can therefore successfully be used to analyse diverse microbial communities from fermented milk products from remote areas.


Assuntos
Produtos Fermentados do Leite/microbiologia , DNA Bacteriano/isolamento & purificação , Microbiologia de Alimentos/métodos , Microbiota , Preservação Biológica/métodos , Análise de Sequência de DNA/métodos , Manejo de Espécimes/métodos , Microbiologia de Alimentos/normas , Preservação Biológica/normas , Análise de Sequência de DNA/normas , Manejo de Espécimes/normas , Zâmbia
17.
Knee Surg Sports Traumatol Arthrosc ; 27(6): 1882-1890, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30888445

RESUMO

PURPOSE: Allografts are frequently use for ligamentous reconstruction at the knee. In the United States, tissue donation and distribution are highly regulated processes with thorough oversight from private and government entities. Allograft is widely available in the United States and allograft procurement is a large industry with varying procurement, sterilization, processing, and distribution procedures. It is important to understand allograft regulation and processing which may affect graft mechanical properties and biological graft integration. METHODS: English-language literature, United States government and regulatory agency statues pertaining to allograft procurement, distribution, and usage were reviewed and the findings summarized. RESULTS: During the processing of allograft, multiple factors including sterilization procedures, irradiation, storage conditions, and graft type all affect the biomechanical properties of the allograft tissue. Biological incorporation and ligamentization of allograft does occur, but at a slower rate compared with autograft. For ligamentous reconstruction around the knee, allograft offers shorter operative time, no donor-site morbidity, but has shown an increased risk for graft failure compared to autograft. CONCLUSION: This article reviews the regulations on graft tissue within the United States, factors affecting the biomechanics of allograft tissue, differences in allograft tissue choices, and the use of allograft for anterior cruciate ligament reconstruction and multiligamentous knee injury reconstruction. LEVEL OF EVIDENCE: V.


Assuntos
Aloenxertos , Regulamentação Governamental , Articulação do Joelho/cirurgia , Tendões/transplante , Transplante Homólogo , Humanos , Ligamentos Articulares/lesões , Ligamentos Articulares/cirurgia , Manejo de Espécimes/normas , Esterilização/métodos , Coleta de Tecidos e Órgãos/legislação & jurisprudência , Obtenção de Tecidos e Órgãos/legislação & jurisprudência , Transplante Homólogo/legislação & jurisprudência , Estados Unidos
18.
Recenti Prog Med ; 110(2): 68-74, 2019 Feb.
Artigo em Italiano | MEDLINE | ID: mdl-30843531

RESUMO

Smart Specialization Strategy (S3) of Lazio defines smart specialization strategies to bring out the excellence of the territory with prospects of success on the global market. Chemical-pharmaceutical, biomedical and biotechnological field is one of the 7 sectors considered of greatest interest for the S3. Key engine of biotechnology development are biological materials and associated data, stored in biobanks. However, to ensure that the research and product development carried out with that resources gives statistically significant and reproducible results, it is essential that they are collected, manipulated and stored using standardized and traced methods. Implementation of the recent published standard ISO 20387- "Biotechnology-Biobanking-General requirements for biobanking" is bridging biobanks toward to storage and distribution of qualified biological material only. Human biobanks are also an essential part of the assistance and care of the citizen and constitute an unavoidable cost of the regional health system. However, biobanks organization, rationalization of their territorial distribution, completion of the process of recognition and regional accreditation, parallel to the implementation of the offer of remunerated services for biobanking, can turn the cost of the necessary preservation of the samples, into an opportunity of territorial development. The paper describes the necessity, shared by a working group represented by several Lazio biobanks, of including biobank activities in the virtuous circle designed by the S3,concretizing the framework prefigured by the S3 document on infrastructures for research, innovation and technology transfer. To allow inclusion of biobank activities in the virtuous circle, we underline the need to quickly start the process of recognition of the territorial research biobanks, to implement at regional level the process of optimization and rationalization of the management of biological samples, in accordance with the international harmonization standards and with the territorial indications of sustainability.


Assuntos
Bancos de Espécimes Biológicos/organização & administração , Pesquisa Biomédica/organização & administração , Biotecnologia/organização & administração , Bancos de Espécimes Biológicos/normas , Pesquisa Biomédica/normas , Biotecnologia/normas , Humanos , Itália , Manejo de Espécimes/normas
19.
Biol Res Nurs ; 21(3): 318-334, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30857393

RESUMO

Salivary cortisol is a commonly used biomarker in cancer survivorship research; however, variations in sampling protocols and parameter reporting limit comparisons across studies. Standardized practices to provide rigor and reproducibility of diurnal salivary cortisol sampling and reporting are not well established. Previous systematic reviews examining relationships between diurnal salivary cortisol and clinical outcomes have resulted in mixed findings. It remains unclear which sampling protocols and reporting parameters offer the greatest utility for clinical research. This review examines diurnal salivary cortisol sampling protocols and reporting parameters to evaluate whether a standardized approach is recommended. A comprehensive search of intervention studies among adult cancer survivors including diurnal salivary cortisol resulted in 30 articles for review. Sampling protocols ranged from 1 to 4 days with the majority of studies sampling cortisol for 2 days. Sampling instances ranged from 2 to 7 times per day, with the majority collecting at 4 time points per day. Diurnal cortisol slope and cortisol awakening response (CAR) were the most commonly reported parameters associated with clinical outcomes. Flattened cortisol slopes, blunted CARs, and elevated evening cortisol concentrations were associated with poorer psychosocial and physiological outcomes. Based on our review, we propose that a rigorous, standardized diurnal salivary cortisol sampling protocol should include sampling at key diurnal times across ≥3 consecutive days to report diurnal cortisol parameters (i.e., CAR and slope) and objective measures of participant protocol adherence. Diminishing budgetary resources and efforts to minimize participant burden dictate the importance of standardized cortisol sampling protocols and reporting parameters.


Assuntos
Biomarcadores/análise , Sobreviventes de Câncer , Guias como Assunto , Hidrocortisona/análise , Saliva/química , Manejo de Espécimes/normas , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes
20.
Chemosphere ; 222: 628-636, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30731383

RESUMO

Flow velocity is known to alter passive sampling accuracy. We investigated the POCIS (Polar Organic Chemical Integrative Sampler) with PRC (Performance Reference Compounds) approach and Diffusive Gradients in Thin Films samplers (o-DGT) to limit the effect of flow on the quantification accuracy of ten model pharmaceuticals compounds (0.16 ≤ log KOW ≤ 4.51). POCIS and o-DGT samplers were exposed for seven days in controlled pilot-scale (hundreds of liters) experiments under quiescent or flowing (2 < V < 18 cm s-1) conditions. Under flowing conditions, both POCIS-PRC and o-DGT efficiently limited the flow effect and led, in most cases, to biases within analytical uncertainty (20%). Under quiescent conditions, o-DGT performed accurately (bias < 30% for most compounds) whereas the PRC approach was unsuitable to improve upon the accuracy of POCIS (PRC was unable to desorb). Therefore, both approaches are helpful in limiting the effects of flow on accuracy, but only o-DGT is efficient in quiescent conditions. However, o-DGT currently suffers from poorer sensitivity compared to POCIS, but the future development of o-DGT devices with wider windows could overcome this limitation.


Assuntos
Projetos Piloto , Manejo de Espécimes/métodos , Poluentes Químicos da Água/análise , Compostos Orgânicos/análise , Preparações Farmacêuticas/análise , Manejo de Espécimes/normas
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