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1.
Food Chem ; 346: 128925, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33418416

RESUMO

Mango is a tropical fruit presenting intense postharvest metabolism. In storage at room temperature, it presents a short shelf life due to the high respiratory rate, and consequent ripening, which limits the marketing period in distant regions. This study evaluated the effect of edible coatings of hydroxypropyl methylcellulose and beeswax in concentrations of 10, 20, and 40% in 'Palmer' mangoes stored for 15 days at 21 °C. The coatings controlled ripening, maintaining peel and pulp colors, firmness, soluble solids (SS), titratable acidity (TA), SS/TA ratio, sugars, ascorbic acid, phenolic compounds, flavonoids, ß-carotene, and antioxidant activity. Also, they reduced weight loss, oxidative stress, and the anthracnose (Colletotrichum gloeosporioides) incidence, without inducing alcohol dehydrogenase activity, which suggests that coated fruit did not ferment. Treatment with 20% beeswax was the most suitable for industrial applications, increasing in six days the mangoes shelf life.


Assuntos
Filmes Comestíveis , Conservação de Alimentos/métodos , Derivados da Hipromelose/química , Derivados da Hipromelose/farmacologia , Mangifera/química , Ceras/química , Colletotrichum/efeitos dos fármacos , Cor , Frutas/química , Frutas/efeitos dos fármacos , Mangifera/microbiologia
2.
Food Chem ; 338: 128117, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33091985

RESUMO

Sugars are critical components of fruit juice. The binding of sugars and aroma compounds may affect the release of flavor in juices. In this study, the effects of sucrose, glucose, and fructose on the release of active aroma compounds in fresh and high hydrostatic pressure-processed (HPP) Tainong mango juice were evaluated. Using gas chromatography-mass spectrometry/olfactometry and odor activity values, four volatiles were identified as active aroma compounds in fresh and HPP mango juice. Furthermore, Fourier transform infrared spectroscopy and isothermal titration calorimetry illustrated that the sugar-volatile interaction was hydrophobically-driven, with hydrogen bonding to some extent. As the concentrations of sugar were increased in the fresh juice, a significant change in the release of active volatiles occurred, while HPP juice showed stronger retention of the active volatiles. The results suggest that high hydrostatic pressure processing maintained the freshness of mango juice flavor by strengthening aroma retention within the juice matrix.


Assuntos
Manipulação de Alimentos , Sucos de Frutas e Vegetais/análise , Mangifera/química , Odorantes/análise , Açúcares/química , Cromatografia Gasosa-Espectrometria de Massas , Pressão Hidrostática , Paladar
3.
Food Chem ; 337: 127764, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-32795857

RESUMO

Mango seed kernel is a by-product which is usually discarded. However, it has been confirmed in this study that seed kernel exhibits more phenolic compounds with bioactive properties than edible fraction of mango. The influence of factors such as cultivar and maturation degree on the phenolic composition has been studied to evaluate nutraceutical value. The comprehensive analysis of phenolic composition by HPLC-DAD-Q-ToF-MS seed kernel from different cultivars ('Keitt', 'Kent'and 'Osteen') at five maturation stages was conducted. Results evidenced that 'Keitt' samples exhibited higher quantities of iriflophenone glucoside, maclurin C-glucoside, maclurin digalloyl glucoside, mangiferin, 5-galloyl quinic acid and trigalloyl glucose at the first three ripening stages. However, seed kernel from 'Osteen' variety showed higher amounts of hexa- and hepta-gallotannins whose concentrations diminished over the maturation course. Therefore, cultivar and maturation stage factors should be take into account due to their influence on the phenolic composition and subsequently on the nutraceutical value.


Assuntos
Cromatografia Líquida de Alta Pressão , Suplementos Nutricionais/análise , Mangifera/química , Espectrometria de Massas , Fenóis/química , Frutas/química , Frutas/metabolismo , Glucosídeos/análise , Glucosídeos/metabolismo , Mangifera/metabolismo , Fenóis/análise , Extratos Vegetais/química
4.
J Environ Sci Health B ; 55(10): 865-875, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32657214

RESUMO

This research aimed at implementing and validating a method for analysis of pesticide residues in crops. QuEChERS extraction method with PSA purification was used following analyzes by gas chromatography with tandem mass spectrometry in Selected Reaction Monitoring mode. A short run method was successfully developed for the determination of 41 pesticides, confirmed by two precursor-products for each analyte. The calibration curve for each analyte was linear at concentration range from 1 to 500 µg kg-1 with correlation coefficients higher than 0.99, low limits of detection (0.03 - 10.22 µg kg-1) and satisfactory precision. The developed method was used to investigate apples; mangos; strawberries; cucumbers and tomatoes from the Rio de Janeiro Food Distribution Center (CEASA).Most of the targeted pesticides (78%) were below detection limits. Apple and strawberry presented the highest pesticide contamination levels, many of which are not authorized by tthe Brazilian national regulatory agency (ANVISA).


Assuntos
Produtos Agrícolas/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Resíduos de Praguicidas/análise , Piretrinas/análise , Extração em Fase Sólida/métodos , Espectrometria de Massas em Tandem/métodos , Triazinas/análise , Brasil , Cucumis sativus/química , Contaminação de Alimentos/análise , Fragaria/química , Lycopersicon esculentum/química , Malus/química , Mangifera/química
5.
Food Chem ; 320: 126608, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32229396

RESUMO

The postharvest ripening behaviour of mangoes (Mangifera indica L.) and particularly the development of colour, volatiles, sensory properties and texture, were investigated. Mangoes cv. Kent from Peru were arranged in a postharvest ripening chamber in two different ways enabling different ventilation of the fruits. Fruit properties were investigated in comparison to reference fruits after postharvest ripening for 78 h. Volatile compounds were analysed by HS-SPME GC-MS; an expert panel performed sensory analysis using descriptive methods. The arrangement of the mangoes significantly impacted the ripening procedures. Dense fruit arrangement induced a degradation of terpenes, a reduced formation of reaction products from the lipoxygenase pathway and less pronounced fruitiness and mango flavour. Principal component analysis based on volatile compounds and sensory properties showed a high correlation with the position in the ripening chamber. These data demonstrate the urgent need for further investigations of the postharvest ripening processes to increase mango quality.


Assuntos
Aromatizantes/análise , Frutas/química , Mangifera/química , Compostos Orgânicos Voláteis/análise , Manipulação de Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Peru , Paladar , Terpenos/análise , Terpenos/química , Terpenos/metabolismo
6.
J Chem Ecol ; 46(4): 387-396, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32274623

RESUMO

Attractive Toxic Sugar Baits (ATSB) are used in a "lure-and-kill" approach for management of the malaria vector Anopheles gambiae, but the active chemicals were previously unknown. Here we collected volatiles from a mango, Mangifera indica, juice bait which is used in ATSBs in Tanzania and tested mosquito responses. In a Y-tube olfactometer, female mosquitoes were attracted to the mango volatiles collected 24-48 h, 48-72 h and 72-96 h after preparing the bait but volatiles collected at 96-120 h were no longer attractive. Volatile analysis revealed emission of 23 compounds in different chemical classes including alcohols, aldehydes, alkanes, benzenoids, monoterpenes, sesquiterpenes and oxygenated terpenes. Coupled GC-electroantennogram (GC-EAG) recordings from the antennae of An. gambiae showed robust responses to 4 compounds: humulene, (E)-caryophyllene, terpinolene and myrcene. In olfactometer bioassays, mosquitoes were attracted to humulene and terpinolene. (E)-caryophyllene was marginally attractive while myrcene elicited an avoidance response with female mosquitoes. A blend of humulene, (E)-caryophyllene and terpinolene was highly attractive to females (P < 0.001) when tested against a solvent blank. Furthermore, there was no preference when this synthetic blend was offered as a choice against the natural sample. Our study has identified the key compounds from mango juice baits that attract An. gambiae and this information may help to improve the ATSBs currently used against malaria vectors.


Assuntos
Anopheles/fisiologia , Controle de Insetos , Mangifera/química , Feromônios/metabolismo , Compostos Orgânicos Voláteis/metabolismo , Animais , Quimiotaxia , Comportamento Alimentar , Feminino , Cadeia Alimentar
7.
Int J Nanomedicine ; 15: 181-197, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32021173

RESUMO

Purpose: The overarching objective of this investigation was to investigate the intervention of green nanotechnology to transform the ancient holistic Ayurvedic medicine scientifically credible through reproducible formulations and rigorous pre-clinical/clinical evaluations. Methods: We provide, herein, full details: (i) on the discovery and full characterization of gold nanoparticles-based Nano Swarna Bhasma (henceforth referred to as NSB drug); (ii) In vitro anti-tumor properties of NSB drug in breast tumor cells; (iii) pre-clinical therapeutic efficacy studies of NSB drug in breast tumor bearing SCID mice through oral delivery protocols and (iv) first results of clinical translation, from mice to human breast cancer patients, through pilot human clinical trials, conducted according to the Ayurveda, Yoga and Naturopathy, Unani, Siddha and Homoeopathy (abbreviated as AYUSH) regulatory guidelines of the Government of India in metastatic breast cancer patients. Results: The preclinical in vitro and in vivo investigations, in breast tumor bearing mice, established unequivocally that the NSB Nano-Ayurvedic medicine-gold nanoparticles-based drug is highly effective in controlling the growth of breast tumors in a dose dependent fashion in vivo. These encouraging pre-clinical results prompted us to seek permission from the Indian Government's holistic medicine approval authority, AYUSH, for conducting clinical trials in human patients. Patients treated with the NSB drug capsules along with the "standard of care treatment" (Arm B) exhibited 100% clinical benefits when compared to patients in the treatment Arm A, thus indicating the tremendous clinical benefits of NSB drug in adjuvant therapy. Conclusion: We have succeeded in clinically translating, from mice to humans, in using proprietary combinations of gold nanoparticles and phytochemicals to develop the Nano-Ayurvedic drug: Nano Swarna Bhasma (NSB), through innovative green nanotechnology, for treating human metastatic breast cancer patients.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Ouro/farmacologia , Medicina Ayurvédica/métodos , Nanopartículas Metálicas/química , Administração Oral , Adulto , Animais , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Feminino , Ouro/química , Química Verde , Humanos , Índia , Mangifera/química , Nanopartículas Metálicas/administração & dosagem , Nanopartículas Metálicas/efeitos adversos , Nanopartículas Metálicas/uso terapêutico , Camundongos SCID , Pessoa de Meia-Idade , Nanotecnologia/métodos , Resultado do Tratamento , Xantonas/química , Ensaios Antitumorais Modelo de Xenoenxerto , Adulto Jovem
8.
Enzyme Microb Technol ; 134: 109479, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32044026

RESUMO

Mangiferin, a major constituent of Mangifera indica L., has attracted substantial attention due to its anti-oxidant, anti-diabetic, anti-inflammatory, and anti-microbial activities. However, its poor solubility in water limits its use in food and pharmaceutical industries. In this study, novel mangiferin-(1→6)-α-d-glucopyranoside (Mg-G1) was enzymatically synthesized from mangiferin and sucrose using glucansucrase from Leuconostoc mesenteroides B-512F/KM, and optimized using response surface methodology. The water solubility of Mg-G1 was found to be 824.7 mM, which is more than 2300-fold higher than that of mangiferin. Mg-G1 also showed DPPH radical scavenging activity and superoxide dismutase (SOD)-like scavenging activity, which were 4.77- and 3.71-fold higher than that of mangiferin, respectively. Mg-G1 displayed inhibitory activity against human intestinal maltase and COX-2. Thus, the novel glucosylated mangiferin may be used as an ingredient in functional food and pharmaceutical application.


Assuntos
Glucosídeos/biossíntese , Glicosiltransferases/metabolismo , Leuconostoc mesenteroides/enzimologia , Mangifera/química , Xantonas/metabolismo , Antioxidantes/metabolismo , Inibidores de Ciclo-Oxigenase 2/metabolismo , Humanos , Solubilidade , Sacarose/metabolismo , Superóxido Dismutase/metabolismo , alfa-Glucosidases/metabolismo
9.
PLoS One ; 15(1): e0227105, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31914140

RESUMO

BACKGROUND: Diabetes mellitus is one of the most common todays public health problems. According to a survey by the World Health Organization, this metabolic disorder has reached global epidemic proportions, with a worldwide prevalence of 8.5% in the adult population. OBJECTIVES: The present study aimed to investigate the hypoglycemic effect of aqueous extract of Mangifera indica (EAMI) leaves in streptozotocin-induced diabetic rats. METHODS: Sixty male rats were divided into 2 groups: Normoglycemic and Diabetic. Each group was subdivided into negative control, glibenclamide 3 or 10 mg/kg, EAMI 125, 250, 500, and 1000 mg/kg. Intraperitoneal injection of streptozotocin 100 mg/kg was used to DM induction. The hypoglycemic response was assessed acutely after two and four weeks of treatment. After a 6-hour fasting period, the fasting blood glucose of animals was verified, and 2.5 g/kg glucose solution was orally administered. The insulin tolerance test and plasma insulin levels assessment were performed in the morning after fasting of 12 to 14 hours. RESULTS AND CONCLUSION: The chemical analysis of EAMI showed high levels of phenolic compounds. There was no significant difference in fasting blood glucose between normoglycemic and diabetic groups, and that EAMI did not have an acute effect on diabetes. After two and four weeks of treatment, the extract significantly reduced blood glucose levels, exceeding glibenclamide effects. EAMI was effective in maintaining the long-term hypoglycemic effect, as well as, significantly increased the sensitivity of diabetic animals to insulin and the plasma insulin level.


Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Hipoglicemiantes/uso terapêutico , Resistência à Insulina , Extratos Vegetais/uso terapêutico , Animais , Glicemia/análise , Diabetes Mellitus Experimental/sangue , Hipoglicemiantes/química , Insulina/sangue , Masculino , Mangifera/química , Extratos Vegetais/química , Ratos Wistar
10.
Food Chem ; 312: 126042, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31911351

RESUMO

Although mango leaves are the main ingredients in some traditional Chinese medicine preparations and folk tea, they with considerable quantities are usually discarded as agricultural waste. Thus, to extend their potential, reverse ultrafiltration-HPLC-DAD-QTOF-MS/MS combining with key ion filtering strategy was proposed to efficiently fish and systematically identify tyrosinase inhibitors in ethyl acetate fraction of mango leaves, which has the highest total phenolic content (40.00 ± 0.84 mg GAE/g DW) and tyrosinase inhibition activity (IC50, 17.62 ± 1.26 µg/mL). Finally, 36 polyphenolic tyrosinase inhibitors were unambiguously characterized or tentatively identified, and three of them were found in mango leaves for the first time. Results suggested that the proposed strategy was powerful for effective identification of bioactive compounds in complex mixtures (e.g. food, agricultural and sideline products), and the findings would lay a foundation for potential applications of mango leaves in pharmaceutical, cosmetic, and food industrial fields.


Assuntos
Inibidores Enzimáticos/farmacologia , Mangifera/química , Monofenol Mono-Oxigenase/antagonistas & inibidores , Cromatografia Líquida de Alta Pressão , Fenóis/análise , Folhas de Planta/química , Espectrometria de Massas em Tandem
11.
Food Sci Technol Int ; 26(1): 11-20, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31370696

RESUMO

Human ingestion of antimicrobial residues in dairy products is a problem of public health. In this study we evaluated antibacterial effectiveness of aqueous and ethanolic leaf extracts of Mangifera indica L. as a natural alternative for reduction of Staphylococcus spp. biofilms. Eight Staphylococcus spp. strains from cows with mastitis and the Staphylococcus aureus strain (ATCC 25923) were evaluated. High performance liquid chromatography indicated the presence of phenolic compounds in the aqueous extract. Agilent Technologies gas chromatography revealed the presence of phenolic compounds, carbohydrates, and gallic acid in ethanolic extract. The tannin contents were 2.27 ± 0.01 and 2.67 ± 0.01% for ethanolic extract and aqueous extract, respectively. Extracts were evaluated in agar diffusion test and the size of inhibition zones were compared. The ethanolic extract was the most effective, showing minimum inhibitory concentrations and minimum bactericidal concentrations between 1.8-7.5 and 15.1-45.3 mg/ml, respectively. After tannin removal, the extracts were less effective, indicating these secondary metabolites as one of the main antibacterial compounds. The ethanolic extract at 45.3 mg/ml reduced mature biofilms in teat rubber (average: 3.91 log reduction) and stainless steel (average: 3.87 log reduction) after 5 min of contact. The extracts of M. indica leaves represent natural alternatives against Staphylococcus spp. strains and the ethanolic extract shows potential as a natural sanitizer.


Assuntos
Antibacterianos/farmacologia , Biofilmes/efeitos dos fármacos , Mangifera/química , Extratos Vegetais/farmacologia , Staphylococcus/efeitos dos fármacos , Animais , Bovinos , Equipamentos e Provisões/microbiologia , Feminino , Mastite Bovina/microbiologia , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Borracha , Aço Inoxidável , Infecções Estafilocócicas/microbiologia , Taninos/análise
12.
Food Sci Technol Int ; 26(1): 78-92, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31466477

RESUMO

The present study investigates recovery of polyphenolic compounds from ripe mango (Mangifera indica L.) peel using deep eutectic solvents based on microwave-assisted extraction method. Lactic acid/sodium acetate/water (3:1:4) screened out from eight different types of deep eutectic solvent systems was used as extractant. A Box-Behnken design along with response surface methodology was applied to optimize the effect of microwave power (W), time (min), and liquid-to-solid ratio (mL g-1) on polyphenol extraction. The optimized conditions determined were power of 436.45 W, time of 19.66 min, and liquid-to-solid ratio of 59.82 mL g-1. Under the optimal conditions, the recovery of total phenolic content, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl scavenging activity was 56.17 mg gallic acid equivalent g-1 dw, 683.27 µmol ascorbic acid equivalent g-1 dw, and 82.64 DPPHsc%, respectively. High Performance Liquid Chromatography (HPLC) analysis revealed mangiferin as the prominent phenolic compound in the mango peel extracts. Microwave-assisted deep eutectic solvent extraction showed remarkable effects on the extraction efficiency of phenolic compounds as revealed from scanning electron microscopy analysis. Rancimat test results revealed that the oxidative stability almost doubled upon addition of purified mango peel extracts to the sunflower oil and thus paving way for the use of mango peel waste as a potential source of antioxidants.


Assuntos
Antioxidantes/isolamento & purificação , Mangifera/química , Micro-Ondas , Extratos Vegetais/química , Polifenóis/isolamento & purificação , Antioxidantes/análise , Compostos de Bifenilo , Fracionamento Químico , Frutas/química , Picratos , Polifenóis/análise
13.
Food Addit Contam Part B Surveill ; 13(1): 16-24, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31631797

RESUMO

This survey describes the occurrence of Ethephon (ETH) and Fosetyl in fruits intended for export produced in the São Francisco Valley, Brazil. The determination of these compounds was carried out by the QuPPe-method (Quick Polar Pesticides Method), which was optimised and successfully validated according to the SANTE/11813/2017. From January 2016 to December 2018 a total of 1048 samples were analysed for ETH residues. In 547 (53%) of the samples, residues below the EU MRL of 1 mg kg-1 were present. In 17 samples (2%) ETH residues were above 1 mg kg-1. Overall, the mean ETH level decreased after 2016. Analyses of fosetyl showed that (18%) of 109 mango samples were positive for this compound. Phosphonic acid was found in concentrations ranging from 0.12 to 3.2 mg kg-1 and 5% of the measurements were above the EU MRL of 2 mg kg-1. The results emphasise the quality control of fruits produced in this region.


Assuntos
Contaminação de Alimentos/análise , Frutas/química , Mangifera/química , Compostos Organofosforados/análise , Resíduos de Praguicidas/análise , Brasil , Fungicidas Industriais/análise , Reguladores de Crescimento de Planta/análise
14.
Food Chem ; 305: 125477, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31610424

RESUMO

In recent years there is an increasing interest in dried fruits, although many of their nutritional components are readily lost during drying. The novelty of this paper was to assess the effect of hot-air drying (HAD) and far-infrared drying (FIRD) on the physicochemical properties and microstructure of mango slices pretreated with: control; 0.1% citric acid + 0.5% ascorbic acid + 0.5% CaCl2 + 1% NaCl; or 0.1% citric acid + 0.5% ascorbic acid + 0.5% CaCl2 + 1% NaCl + ultrasound treatments. Pretreatments resulted in a significant (p < 0.05) increase in reducing sugar, ascorbic acid and total phenol contents of dried samples. Moreover, compared with HAD, FIRD maintained a higher phenolic acid content, improved the nutrient retention and color attributes and better preserved microstructure. Therefore, pretreatments followed by FIRD is a potential method for obtaining high-quality dried mango slices.


Assuntos
Ácido Ascórbico/análise , Dessecação/métodos , Mangifera/química , Fenóis/análise , Ar , Cloreto de Cálcio/química , Cromatografia Líquida de Alta Pressão , Cor , Frutas/química , Frutas/metabolismo , Temperatura Alta , Raios Infravermelhos , Mangifera/metabolismo , Mangifera/ultraestrutura , Espectrometria de Massas , Sonicação
15.
Phytother Res ; 34(3): 505-525, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31755173

RESUMO

Osteoarthritis (OA) pain has been proposed to be a mixed pain state, because in some patients, central nervous system factors are superimposed upon the more traditional peripheral factors. In addition, a considerable amount of preclinical and clinical evidence has shown that, accompanying the central neuroplasticity changes and partially driven by a peripheral nociceptive input, a real neuropathic component occurs that are particularly linked to disease severity and progression. Hence, innovative strategies targeting neuroprotection and particularly neuroinflammation to prevent and treat OA pain could be introduced. Mangiferin (MG) is a glucosylxanthone that is broadly distributed in higher plants, such as Mangifera indica L. Previous studies have documented its analgesic, anti-inflammatory, antioxidant, neuroprotective, and immunomodulatory properties. In this paper, we propose its potential utility as a multitargeted compound for mixed OA pain, even in the context of multimodal pharmacotherapy. This hypothesis is supported by three main aspects: the cumulus of preclinical evidence around this xanthone, some preliminary clinical results using formulations containing MG in clinical musculoskeletal or neuropathic pain, and by speculations regarding its possible mechanism of action according to recent advances in OA pain knowledge.


Assuntos
Analgésicos/uso terapêutico , Mangifera/química , Neuralgia/tratamento farmacológico , Osteoartrite/complicações , Xantonas/uso terapêutico , Humanos , Hiperalgesia/etiologia , Neuralgia/etiologia , Neuralgia/prevenção & controle , Neuroproteção/efeitos dos fármacos
16.
Int J Pharm ; 573: 118844, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31751638

RESUMO

Mangiferin, a natural compound isolated from Mangifera indica L, was incorporated in glycerosomes, ethosomes and alternatively in glycerol-ethanol phospholipid vesicles (glycethosomes). Actually, only glycethosomes were able to stably incorporate the mangiferin that was loaded at increasing concentrations (2, 4, 6, 8 mg/mL). The morphology, size distribution, rheological properties, surface charge and entrapment efficiency of prepared vesicles were deeply measured. All vesicles were mainly spherical, oligolamellar, small in size (~145 nm) and negatively charged (~-40 mV), as confirmed by cryo-TEM observation and dynamic laser light scattering measurements. The higher concentration of mangiferin (8 mg/mL) allowed an increase of vesicle mean diameter up to ~288 nm. The entrapment efficiency was inversely proportional to the amount of loaded mangiferin. In vitro studies performed by using human abdominal skin, underlined that, the dose-dependent ability of vesicles to promote mangiferin retention in epidermis. In addition, glycethosomes were highly biocompatible and showed a strong ability to protect in vitro the fibroblasts against damages induced by hydrogen peroxide. In vivo results underlined the superior ability of mangiferin loaded glycethosomes respect to the mangiferin dispersion to promote the heal of the wound induced by TPA, confirming their potential application for the treatment of psoriasis or other skin disorders.


Assuntos
Adjuvantes Farmacêuticos/administração & dosagem , Portadores de Fármacos/química , Mangifera/química , Psoríase/tratamento farmacológico , Xantonas/administração & dosagem , Células 3T3 , Adjuvantes Farmacêuticos/farmacocinética , Administração Cutânea , Animais , Modelos Animais de Doenças , Composição de Medicamentos/métodos , Epiderme/efeitos dos fármacos , Epiderme/metabolismo , Etanol/química , Feminino , Glicerol/química , Humanos , Peróxido de Hidrogênio/toxicidade , Camundongos , Fosfolipídeos/química , Psoríase/induzido quimicamente , Acetato de Tetradecanoilforbol/toxicidade , Distribuição Tecidual , Cicatrização/efeitos dos fármacos , Xantonas/farmacocinética
17.
Spectrochim Acta A Mol Biomol Spectrosc ; 229: 117913, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31855814

RESUMO

The assessment of pesticide residue levels demands fast, low cost and easy-to-use procedures which are not found in conventional methods. In this work, SERS substrates based on the deposition of gold nanoparticles (GNPs) on common office paper were prepared using a wax printer. These substrates combined with Data Driven Soft Independent Modelling of Class Analogies (DD-SIMCA), a one-class classifier algorithm, were used for detection of pesticide residues in water extracts of mango peels. Paper-based substrates made sample collection easier compared with conventional SERS methods, since few microliters of the pesticide aqueous extract from fruit peels needed to be deposited onto the substrate. Moreover, one-class classifiers dismiss the need for quantification or calibration curves. Classification of a fruit with residue levels in accordance to regulatory bodies' limits is based on a mathematical threshold. Just as in an authentication problem, all the possibilities for a given analysed fruit are now restricted to agreeing or not agreeing with current regulations. The performance of the one-class model was demonstrated by detecting thiabendazole (TBZ) residues at various mango samples, with all results being confirmed by HPLC-DAD analysis. The final model could distinguish samples with TBZ levels above the ones allowed by the Brazilian Health Regulatory Agency with 94% of selectivity and 92% of sensitivity, even in the presence of other pesticides.


Assuntos
Mangifera/química , Papel , Resíduos de Praguicidas/análise , Extratos Vegetais/química , Análise Espectral Raman/métodos , Tiabendazol/análise , Resíduos/análise , Algoritmos , Padrões de Referência , Reprodutibilidade dos Testes
18.
Food Chem ; 311: 126057, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-31869636

RESUMO

Fermented foods have been consumed for centuries in various parts of the world and are known to be rich resources of functionally important microorganisms. This study documents the antioxidative, anticancer and enzyme-inhibiting properties of potential probiotic Bacillus strains isolated from fermented brine mango pickle. Antioxidant activity was determined through in-vitro assays namely, DPPH•, ABTS•+, hydroxyl radical scavenging ability, reducing activity, superoxide anion scavenging ability, linoleic acid and plasma lipid peroxidation ability. Both intact cells (IC) and intracellular cell-free extracts (CFE) from most of the strains exhibited prominent antioxidant activity. Likewise, CFE and intracellular cell-free supernatants (CFS) exhibited potential inhibitory activities towards α-amylase, α-glucosidase and tyrosinase. Interestingly, CFS and crude ethyl acetate extracts of PUFSTP35 (Bacillus licheniformis KT921419) displayed strong anticancer activity against HT-29 colon cancer cell line. Hence, these probiotic strains have been showed to exhibit unique functional properties and could be further commercially exploited.


Assuntos
Antineoplásicos/farmacologia , Antioxidantes/farmacologia , Inibidores de Glicosídeo Hidrolases/farmacologia , Mangifera/química , Probióticos/farmacologia , Bacillus/efeitos dos fármacos , Fermentação , Células HT29 , Humanos , Mangifera/microbiologia , Monofenol Mono-Oxigenase/antagonistas & inibidores , Sais , alfa-Amilases/antagonistas & inibidores , alfa-Glucosidases
19.
Arch Oral Biol ; 109: 104554, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31563709

RESUMO

OBJECTIVE: Psidium sp., Mangifera sp. and Mentha sp. and its mixture (PEM) are known to have antimicrobial and anti-adherence effects. DESIGN: Here, we have investigated these individual plant extracts and its synergistic mixture (PEM) for its anti-cariogenic effect to reduce populations of single and mixed-species of Streptococcus sanguinis and Streptococcus mutans in a planktonic or/and biofilm and their others reduced virulence. Bacterial populations in the biofilm after 24 h, hydrophobic cell surface activity to n-hexadecane and pH changes at 5 min' intervals until 90 min of incubation were recorded. Total phenolic content and bioactive compounds in the crude aqueous plant extracts were analysed. Regulatory gene expressions of S. mutans adhesins genes (gtfB, gtfC, gbpB and spaP) upon treatment with PEM were investigated in planktonic and biofilm conditions. RESULTS: All plant extracts strongly reduced S. mutans in the biofilm compared to S. sanguinis in single and mixed-species. PEM reduced S. mutans by 84% with S. sanguinis 87% in the mixed population. Psidium sp. and PEM highly reduced cell-surface hydrophobicity of the two bacteria thus reducing adherence and biofilm formation. PEM and Mangifera sp. lowered initial pH change in the mixed populations of S. sanguinis and S. mutans. PEM downregulated the S. mutans gtfB gene expression in the single species planktonic and mixed-species biofilms. CONCLUSIONS: The effectiveness of PEM in reducing S. mutans within the biofilm, cell-surface hydrophobicity, acid production and adhesin gene (gtfB) expression in mixed-species with S. sanguinis indicates its potential as an antibacterial agent against dental caries. This is attributed to the phenolic content in the PEM.


Assuntos
Biofilmes/efeitos dos fármacos , Mangifera/química , Mentha/química , Extratos Vegetais/farmacologia , Psidium/química , Ácidos/análise , Aderência Bacteriana/efeitos dos fármacos , Genes Bacterianos , Concentração de Íons de Hidrogênio , Streptococcus/efeitos dos fármacos , Streptococcus mutans/efeitos dos fármacos , Streptococcus sanguis
20.
Molecules ; 24(23)2019 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-31795169

RESUMO

The aim of this study was to develop a nondairy fermented product based on mango slurry. Lactobacillus plantarum and Saccharomyces cerevisiae DV10 were used as starter cultures in single and co-cultivations. The microbial populations and metabolites produced during mango slurry fermentation were investigated. At the end of all fermentations, the bacterial populations were higher than 6.0 log CFU/mL. Lactic acid was the main organic acid produced, achieving up to 6.12 g/L after 24 h in co-culture with L. plantarum and S. cerevisiae DV10. Volatile compounds were determined after 24 h of fermentation, the co-cultures of L. plantarum and S. cerevisiae DV10 could decrease terpenes and produce alcohols and esters. The co-cultivations obtained the most total phenolics as well as showed the strongest 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging activity, ferric-reducing antioxidant power (FRAP) and low-density lipoprotein (LDL) oxidation inhibition. Hence, a high-bioactivity probiotic product was successfully obtained via mango slurry fermentation inoculated with a co-culture of L. plantarum and S. cerevisiae DV10.


Assuntos
Fermentação , Lactobacillus plantarum/metabolismo , Mangifera/química , Saccharomyces cerevisiae/metabolismo , Antioxidantes/química , Antioxidantes/farmacologia , Microbiologia de Alimentos , Oxirredução , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/farmacologia
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