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1.
Isr Med Assoc J ; 22(12): 736-740, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33381943

RESUMO

BACKGROUND: Chronic lung diseases, especially emphysema and pulmonary fibrosis, are the third leading cause of mortality worldwide. Their treatment includes symptom alleviation, slowing of the disease progression, and ultimately organ transplant. Regenerative medicine represents an attractive alternative. OBJECTIVES: To develop a dual approach to lung therapy by engineering a platform dedicated to both remodeling pulmonary architecture (bronchoscopic lung volume reduction) and regeneration of lost respiratory tissue (scaffold). METHODS: The authors developed a hydrogel scaffold based on the natural polymers gelatin and alginate. The unique physical properties allow its injection through long catheters that pass through the working channel of a bronchoscope. The scaffold might reach the diseased area; thus, serving a dual purpose: remodeling the lung architecture as a lung volume reduction material and developing a platform for tissue regeneration to allow for cell or organoid implant. RESULTS: The authors' novel hydrogel scaffold can be injected through long catheters, exhibiting the physical and mechanical properties necessary for the dual treatment objectives. Its biocompatibility was analyzed on human fibroblasts and mouse mesenchymal cells. Cells injected with the scaffold through long narrow catheters exhibited at least 70% viability up to 7 days. CONCLUSIONS: The catheter-injectable gelatin-alginate hydrogel represents a new concept, which combines tissue engineering with minimal invasive procedure. It is an inexpensive and convenient to use alternative to other types of suggested scaffolds for lung tissue engineering. This novel concept may be used for additional clinical applications in regenerative medicine.


Assuntos
Hidrogéis/uso terapêutico , Pneumopatias/terapia , Engenharia Tecidual/métodos , Tecidos Suporte , Alginatos , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/uso terapêutico , Cateteres , Fibroblastos , Gelatina , Humanos , Hidrogéis/administração & dosagem , Injeções , Pulmão , Camundongos
2.
Khirurgiia (Mosk) ; (10): 79-87, 2020.
Artigo em Russo | MEDLINE | ID: mdl-33047590

RESUMO

Active collagen type I successfully used in regenerative medicine. However, despite the large amount of material of cellular and molecular mechanisms underlying skin repair, the molecular mechanisms of wound healing with use collagen type I, not studied enough. PURPOSE OF THE STUDY: To study the mechanism of the native collagen type I wound-healing action of native type I collagen on the example of the medical device Collost (7% gel) in a model of the rats difficult-to-heal skin wounds. MATERIAL AND METHODS: Male rats in population SD (72 individuals) surgically formed an ischemic dorsal skin flap (3×10 cm) with two full-thickness skin wounds 6 mm in diameter.The trained animals divided into 2 groups: in the experimental group, medical device Collost (gel) applied once after the operation, in the control group - a standard medical device for comparison. The dynamics of wound healing assessed, the number of M2 macrophages, myofibroblasts, vascularization and expression of the main markers of the repair process in the wound tissues and time points for assessment were: after 3, 7 and 14 days after operation using macroscopic, immunohistochemical, and molecular methods. RESULTS: It has been established that the mechanism of action of native collagen type I is associated with the acceleration of the appearance of «progenitorous¼ M2-macrophages in the wound tissues, decrease in the severity of inflammation or reduction in the duration of the inflammatory stage of the repair process, change in the expression spectrum of number of growth factors, an acceleration of neovasculogenesis. CONCLUSION: In this work, on the modern experimental model shown regenerative efficiency of a medical device based on collagen type I and described the molecular and cellular processes of wound healing when using it It has been shown that the acceleration of wound healing processes occurs when using a medical device based on native collagen type 1, it is also accompanied by a better aesthetic closure of the damaged skin area.


Assuntos
Colágeno Tipo I/administração & dosagem , Fármacos Dermatológicos/administração & dosagem , Pele/efeitos dos fármacos , Lesões dos Tecidos Moles/tratamento farmacológico , Cicatrização/fisiologia , Animais , Materiais Biocompatíveis/administração & dosagem , Modelos Animais de Doenças , Géis , Isquemia/tratamento farmacológico , Masculino , Ratos , Pele/lesões , Lesões dos Tecidos Moles/terapia , Retalhos Cirúrgicos/irrigação sanguínea
3.
PLoS One ; 15(9): e0239544, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32966339

RESUMO

Injection laryngoplasty (IL) has been used to treat various types of glottal insufficiency. The precise volume and location of the injected materials impact the outcomes. However, exactly how increasing volumes of material are distributed is unknown. In fact, the amount of IL material required to medialize a vocal cord tends to be determined empirically. Thus, the goal of this study was to investigate the pattern of IL material distribution by checking serial micro-computed tomography (MCT) and pressure changes during ILs. This experimental study used 10 excised canine larynges. Experimental devices included the IL syringe, pressure sensor, infusion pump, fixed frame, and monitoring system. We injected calcium hydroxyapatite in the thyroarytenoid muscle; whenever 0.1 mL of material was injected, we obtained an MCT scan while simultaneously measuring the pressure. After the experiments, we performed histologic analyses. MCT analyses showed that materials initially expanded centrifugally and then expanded in all directions within the muscle. The pressure initially increased rapidly but then remained relatively constant until the point at which the materials expanded in multiple directions. Histologic analyses showed that the IL material tended to expand within the epimysium of the thyroarytenoid muscle. However, in some cases, the MCT revealed that there were leakages to the surrounding space with a corresponding pressure drop. If the IL material passes through the epimysium, leakage can occur in the surrounding space, which can account for the reduction in resistance during ILs.


Assuntos
Laringoplastia/métodos , Animais , Materiais Biocompatíveis/administração & dosagem , Cães , Durapatita/administração & dosagem , Técnicas In Vitro , Injeções/efeitos adversos , Injeções/instrumentação , Injeções/métodos , Músculos Laríngeos/diagnóstico por imagem , Músculos Laríngeos/cirurgia , Laringe/diagnóstico por imagem , Laringe/cirurgia , Modelos Animais , Pressão , Prega Vocal/diagnóstico por imagem , Prega Vocal/cirurgia , Microtomografia por Raio-X
4.
Spine (Phila Pa 1976) ; 45(15): E892-E902, 2020 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-32675599

RESUMO

STUDY DESIGN: A multicenter, randomized, open-label, parallel-group trial. OBJECTIVE: To investigate interbody bone fusion rates in titanium-coated polyetheretherketone (TiPEEK) and polyetheretherketone (PEEK) cages after posterior lumbar interbody fusion (PLIF) surgery. SUMMARY OF BACKGROUND DATA: Previous clinical studies have not revealed any significant difference in bone fusion rates between TiPEEK and PEEK cages. METHODS: During one-level PLIF surgery, 149 patients (84 men, 65 women, mean age 67 yr) were randomly allocated to use either a TiPEEK cage (n = 69) or PEEK cage (n = 80). Blinded radiographic evaluations were performed using computed tomography and assessed by modified intention-to-treat analysis in 149 cases and per-protocol analysis in 143 cases who were followed for 12 months. Clinical outcomes were assessed using the Japanese Orthopaedic Association Back Pain Evaluation Questionnaire and the Oswestry Disability Index. RESULTS: The interbody union rate at 12 months after surgery was 45% owing to a very strict definition of bone fusion. The rates of bone fusion were significantly higher at 4 and 6 months after surgery in the TiPEEK group than in the PEEK group in the unadjusted modified intention-to-treat analysis and were significantly higher at 6 months in the unadjusted per-protocol analysis. Binary logistic regression analysis adjusted for sex, age, body mass index, bone mineral density, and surgical level showed that using a TiPEEK cage (odds ratio, 2.27; 95% confidence interval: 1.09-4.74; P = 0.03) was independently associated with bone fusion at 6 months after surgery. Japanese Orthopaedic Association Back Pain Evaluation Questionnaire and Oswestry Disability Index results improved postoperatively in both groups. CONCLUSION: Using the TiPEEK cage for PLIF enabled the maintenance of better bone fusion to the endplate than using the PEEK cage at 6 months after the surgery. Our findings suggest the possibility of an earlier return to rigorous work or sports by the use of TiPEEK cage. LEVEL OF EVIDENCE: 1.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Fixadores Internos , Cetonas/administração & dosagem , Vértebras Lombares/cirurgia , Polietilenoglicóis/administração & dosagem , Fusão Vertebral/métodos , Titânio/administração & dosagem , Adulto , Idoso , Feminino , Humanos , Lactente , Fixadores Internos/tendências , Vértebras Lombares/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Fusão Vertebral/instrumentação
5.
Plast Reconstr Surg ; 146(1): 41-51, 2020 07.
Artigo em Inglês | MEDLINE | ID: mdl-32590640

RESUMO

BACKGROUND: Nonsurgical rhinoplasty using filler injections has become a common procedure in cosmetic practices. This is offered to patients that prefer a temporary outcome or would like to avoid general anesthesia. In addition, it can be used in postrhinoplasty patients to correct nasal deformities or irregularities. This systematic review highlights common filler types and injection techniques, and associated patient satisfaction and complications to further guide practitioners. METHODS: A systematic review was performed using keywords and Medical Subject Headings search terms. PubMed, EmBase, the Cochrane Library, and Scopus were searched using the appropriate search terms. Data collected from each study included patient satisfaction and complications, in addition to injection material, location, and technique. RESULTS: Four thousand six hundred thirty-two studies were found based on search criteria. After full-text screening for inclusion and exclusion criteria, 23 studies were included. A total of 1600 patients underwent nonsurgical rhinoplasty, most commonly with hyaluronic acid (73.38 percent), followed by calcium hydroxyapatite (12.44 percent). Nearly 95 percent of patients were satisfied with results, and there were only 26 relatively minor complications reported. There were no reports of vascular complications such as skin necrosis or visual compromise. CONCLUSIONS: Based on the authors' review of the literature, nonsurgical rhinoplasty is an effective temporary alternative to traditional augmentation rhinoplasty for corrections of nasal shape with a high degree of patient satisfaction. Complications may be underreported, and thus further investigation is needed to better understand the true incidence of major complications related to vascular compromise.


Assuntos
Preenchedores Dérmicos/uso terapêutico , Rinoplastia/métodos , Materiais Biocompatíveis/administração & dosagem , Humanos , Injeções Subcutâneas/métodos , Satisfação do Paciente
6.
Sci Rep ; 10(1): 5824, 2020 04 02.
Artigo em Inglês | MEDLINE | ID: mdl-32242027

RESUMO

Tissue engineering allows to combine biomaterials and seeded cells to experimentally replace urinary bladder wall. The normal bladder wall however, includes branched neuronal network propagating signals which regulate urine storage and voiding. In this study we introduced a novel biocomposite built from amniotic membrane (Am) and graphene which created interface between cells and external stimuli replacing neuronal network. Graphene layers were transferred without modifying Am surface. Applied method allowed to preserve the unique bioactive characteristic of Am. Tissue engineered constructs composed from biocomposite seeded with smooth muscle cells (SMC) derived from porcine detrusor and porcine urothelial cells (UC) were used to evaluate properties of developed biomaterial. The presence of graphene layer significantly increased electrical conductivity of biocomposite. UCs and SMCs showed an organized growth pattern on graphene covered surfaces. Electrical filed stimulation (EFS) applied in vitro led additionally to increased SMCs growth and linear arrangement. 3D printed chamber equipped with 3D printed graphene based electrodes was fabricated to deliver EFS and record pressure changes caused by contracting SMCs seeded biocomposite. Observed contractile response indicated on effective SMCs stimulation mediated by graphene layer which constituted efficient cell to biomaterial interface.


Assuntos
Âmnio/citologia , Materiais Biocompatíveis/administração & dosagem , Grafite/administração & dosagem , Reimplante/métodos , Engenharia Tecidual/métodos , Bexiga Urinária/efeitos dos fármacos , Bexiga Urinária/fisiologia , Animais , Proliferação de Células/efeitos dos fármacos , Condutividade Elétrica/uso terapêutico , Masculino , Contração Muscular/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Suínos , Tecidos Suporte , Urotélio/efeitos dos fármacos
7.
Khirurgiia (Mosk) ; (3): 29-34, 2020.
Artigo em Russo | MEDLINE | ID: mdl-32271734

RESUMO

OBJECTIVE: Experimental assessment of the effect of modified and unmodified surgical suture material on abdominal adhesive process. MATERIAL AND METHODS: The study was performed on male rats of the Wistar subpopulation. There were 5 animals in each group. In all animals, midline abdominal incision was followed by suturing the parietal peritoneum with modified and unmodified suture material. All animals were euthanized with carbon dioxide vapors in 14 days after surgery. Macro- and microscopic assessment of severity of abdominal adhesive process was carried out. Two types of preparation of excised complexes 'peritoneum-suture material-adhesion' were applied for histological examination: paraffin sections and embedding in epoxy resin. Specimens were stained by Van Gieson and with methylene blue solution. Histological specimens were examined using Axio Imager A1 light microscope (Zeiss, Germany). RESULTS: Polypropylene filaments result extensive adhesions occupying about 75% of the area. Adhesions have a dense structure with signs of vascularization. Modification of suture material with solution of polyhydroxybutyrate/hydroxyvalerate and heparin reduce severity of adhesions. The use of modified suture material was followed by adhesions with more loose structure, no signs of vascularization. Adhesions occupied less than 25% of the area. Histological examination of excised complexes 'peritoneum-suture material-adhesion' revealed accumulation of inflammatory cells around the unmodified suture material, while there were no signs of tissue inflammatory process around the modified sutures. CONCLUSION: Application of polyhydroxybutyrate/hydroxyvalerate and heparin on the surface of surgical sutures is an effective method for prevention of abdominal adhesions.


Assuntos
Materiais Biocompatíveis/efeitos adversos , Heparina/administração & dosagem , Poliésteres/administração & dosagem , Polipropilenos/efeitos adversos , Suturas/efeitos adversos , Aderências Teciduais/prevenção & controle , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Revestidos Biocompatíveis/administração & dosagem , Materiais Revestidos Biocompatíveis/efeitos adversos , Modelos Animais de Doenças , Heparina/efeitos adversos , Masculino , Neovascularização Patológica/etiologia , Neovascularização Patológica/prevenção & controle , Peritônio/irrigação sanguínea , Peritônio/patologia , Peritônio/cirurgia , Poliésteres/efeitos adversos , Polipropilenos/administração & dosagem , Ratos , Ratos Wistar , Aderências Teciduais/etiologia , Aderências Teciduais/patologia
8.
In Vivo ; 34(2): 609-614, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32111760

RESUMO

BACKGROUND/AIM: Tooth extraction is a common procedure in dental clinics. Tooth extraction can destroy gingiva, alveolar bone, periodontal ligaments and cement. If dental sockets are left as extracted, it will result in loss of teeth, as well as voice and aesthetic problems. A natural hydroxyapatite (HA) bioceramic bone graft substitute developed from goose-beak bone particles (GBPs) was used for dentoalveolar reconstruction in a canine model. MATERIALS AND METHODS: Four adult (18-22 months old) male beagle dogs weighing 8.2-9.6 kg were included in the study. Eight alveolar extraction sockets in the four dogs were divided randomly into two groups and a split-mouth design was established; control group, socket filled with commercial synthetic HA; tested group, socket filled with granulated GBP. RESULTS: Micro-CT analysis and hematoxylin and eosin and Masson's trichrome staining of non-decalcified sections were undertaken. Examination revealed that dentoalveolar reconstruction was initiated from the periphery of the host bone, and newly formed bone was well integrated with the GBP. Bone apposition was observed at the edge of the host bone-GBP interface. CONCLUSION: A natural ceramic powder obtained from GBP is suitable for use in dentoalveolar reconstruction in dogs.


Assuntos
Bico , Materiais Biocompatíveis , Regeneração Óssea , Substitutos Ósseos , Materiais Dentários , Gansos , Animais , Bico/química , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/química , Substitutos Ósseos/administração & dosagem , Substitutos Ósseos/química , Materiais Dentários/química , Cães , Durapatita/química , Regeneração Tecidual Guiada , Masculino , Osteogênese , Alvéolo Dental/diagnóstico por imagem , Alvéolo Dental/patologia , Microtomografia por Raio-X
9.
Acta Orthop Traumatol Turc ; 54(1): 49-58, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32175897

RESUMO

OBJECTIVE: The aim of this study was to compare the outcome of intra-lesional autologous bone marrow concentrate (BMC) and equine derived demineralized bone matrix (EDDBM) injections with methylprednisolone acetate injections in patients with simple bone cyst. METHODS: Clinical records and radiographs of 53 consecutive patients (37 females,and 16 males; mean age: 10.6±1.53 years) treated between 2006 and 2016 were retrospectively reviewed. Healing was assessed by an independent radiologist according to Neer scoring system. Functional outcome was assessed with the Activity Scale for Kids (ASK). Thirty-four cysts were in the humerus, 13 in the femur and 6 in other locations. Twenty-nine patients were included in Steroid Group and treated with 3 cycles of injections of methylprednisolone acetate, while 24 patients were treated with injection of autologous bone marrow concentrate and equine derived demineralized bone matrix (BMC+ EDDBM Group). The two groups were homogenous for the mean age, sex distribution, cysts location and their clinical presentation. RESULTS: At a minimum follow-up of 24 months, success rate (Neer/Cole score 3 and 4) was higher in EDDBM+BMC group (83.3% vs 58.6%; p=0.047). Female patients had higher healing rates in both groups (p=0.002). No association was found between healing and age (p=0.839), cyst activity (p=0.599), cyst localization (p=0.099) and clinical presentation (p=0.207). BMC+EDDBM group showed higher ASK score (p=0.0007). CONCLUSION: Treatment with BMC+EDDBM injections may provide better results with a single procedure than 3 methylprednisolone acetate injections and represent an interesting alternative for the treatment of unicameral bone cysts. LEVEL OF EVIDENCE: Level III, Therapeutic Study.


Assuntos
Cistos Ósseos , Transplante de Medula Óssea/métodos , Acetato de Metilprednilosona/administração & dosagem , Materiais Biocompatíveis/administração & dosagem , Cistos Ósseos/diagnóstico por imagem , Cistos Ósseos/terapia , Matriz Óssea , Criança , Feminino , Glucocorticoides/administração & dosagem , Humanos , Injeções Intralesionais/métodos , Masculino , Radiografia/métodos , Estudos Retrospectivos , Resultado do Tratamento
10.
BMC Oral Health ; 20(1): 77, 2020 03 18.
Artigo em Inglês | MEDLINE | ID: mdl-32183831

RESUMO

BACKGROUND: The purpose of this study was to compare the effectiveness of absorbable collagen sponge insertion in tooth extraction sites for socket healing of the impacted mandibular third molar. METHODS: Thirty-six patients with bilateral mandibular impacted third molars based on Pell-Gregory and Winter classification were included in this study. This study was a randomized clinical trial utilizing a split-mouth design with one side assigned as collagen sponge insertion and the other side assigned as the control. Post-operative clinical complications, periodontal integrities, and radiographic outcomes were assessed at 1, 2, and 14-weeks post operatively. RESULTS: Five patients were excluded during the follow-up period due to loss of follow-up. The study was conducted on 31 patients in total. The mean VAS score of collagen sponge insertion side at 1 week post operation was 1.42 ± 1.26, which was significantly lower than the control side (P < 0.05). The mean probing depth of collagen sponge insertion side at 2-week post operation was 5.55 ± 2.28 mm, which was significantly lower than the control side (7.13 ± 1.86; P < 0.05). Other various measurements including radiographic outcomes showed no significant group differences. CONCLUSIONS: Placement of collagen sponge after extraction of mandibular impacted third molar reduced early stage post-operative complications and enhanced initial healing of soft tissues and periodontal defects. TRIAL REGISTRATION: This study was retrospectively registered at the WHO ICTRP platform and Clinical Research Information Service, KCT0003363. Registered 21 Sep 2018.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Colágeno/administração & dosagem , Dente Serotino/cirurgia , Tampões de Gaze Cirúrgicos , Extração Dentária , Dente Impactado/cirurgia , Adulto , Feminino , Humanos , Masculino , Mandíbula/cirurgia , Resultado do Tratamento , Técnicas de Fechamento de Ferimentos , Cicatrização
11.
J Photochem Photobiol B ; 205: 111845, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32172137

RESUMO

We report, the one-pot synthesis of water-soluble and biocompatible 3-mercaptopropylsulfonate (MPS) protected novel copper nanoclusters (CuNCs). Interestingly, the TEM image of MPS protected CuNCs exhibits an ultrasmall nanoclusters of particle size <2-nm, similar to its Au and Ag analogue. The hydrophilic and biocompability property of thiolate protected CuNCs. i.e., MPS stabilized CuNCs and its luminescent nature gave rise to maximum quantum yield of 1.5%. Further, as achieved CuNCs was investigated for haemocompatibility, cell viability and fluorescent microscopic analysis with A549 lung cancer cell line. Haemolytic study was examined using human RBCs in the concentration range of 4 to 22 µg/mL for which 7.5% of haemolysis was obtained for an optimum concentration of 22 µg/mL of CuNCs. The cell viability analysis was carried out by MTT assay using A549 lung cancer cells for the minimum (10 µg/mL) and maximum (45 µg/mL) concentration of CuNCs which reports 93.1% and 38.2% cell viability respectively. The inverted light microscopic images from the control and CuNCs treated (20 µg/mL) cells exhibited an excellent biocompatibility with a normal morphology. Upon increasing the concentration of CuNCs upto 45 µg/mL, the cell viability trends to decrease and the cell morphology also denature gradually. Further, the bio-imaging application of CuNCs was analyzed with A549 lung cancer cells. The efficient imaging with CuNCs treated (20 µg/mL) A549 cells resulted in a green colour emission using FITC filter (460- 490 nm). Thereby the obtained results confirm the applicability of CuNCs for the biomedical and cancer diagnosis applications.


Assuntos
Materiais Biocompatíveis , Cobre , Neoplasias Pulmonares/diagnóstico por imagem , Nanoestruturas , Compostos de Sulfidrila , Ácidos Sulfônicos , Células A549 , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/química , Sobrevivência Celular/efeitos dos fármacos , Cobre/administração & dosagem , Cobre/química , Eritrócitos/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Humanos , Nanoestruturas/administração & dosagem , Nanoestruturas/química , Compostos de Sulfidrila/administração & dosagem , Compostos de Sulfidrila/química , Ácidos Sulfônicos/administração & dosagem , Ácidos Sulfônicos/química
12.
J Laryngol Otol ; 134(3): 263-269, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32127077

RESUMO

BACKGROUND: Vocal fold injection augmentation is a recognised treatment modality for glottic insufficiency. Causes of glottal closure insufficiency include vocal fold paralysis, paresis, atrophy, sulcus vocalis, scarring and vocal fold deficiency after laryngeal surgery. A variety of materials exist for injection augmentation. This study aimed to compare voice improvement after injection augmentation between two injectable materials: carboxymethyl cellulose and calcium hydroxyapatite. METHOD: This retrospective study included 66 consecutive patients with glottic insufficiency who underwent injection augmentation. RESULTS: Among the patients who received their first injection augmentation with carboxymethyl cellulose and their second injection augmentation with calcium hydroxyapatite (n = 28), voice quality improved significantly after both injection augmentations. No significant differences were observed in any of the objective and subjective voice quality measurements examined following carboxymethyl cellulose and calcium hydroxyapatite injections. CONCLUSION: Voice improvement after injection augmentation depends mainly on the improvement of glottic closure, rather than the injection material.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Carboximetilcelulose Sódica/administração & dosagem , Durapatita/administração & dosagem , Disfunção da Prega Vocal/cirurgia , Voz/efeitos dos fármacos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Glote/fisiopatologia , Glote/cirurgia , Humanos , Injeções , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
13.
Sci Rep ; 10(1): 3367, 2020 02 25.
Artigo em Inglês | MEDLINE | ID: mdl-32099052

RESUMO

Complex abdominal wall defects (CAWDs) can be difficult to repair and using a conventional synthetic mesh is often unsuitable. A biological mesh might offer a solution for CAWD repair, but the clinical outcomes are unclear. Here, we evaluated the efficacy of a cross-linked, acellular porcine dermal collagen matrix implant (Permacol) for CAWD repair in a cohort of 60 patients. Here, 58.3% patients presented with a grade 3 hernia (according to the Ventral Hernia Working Group grading system) and a contaminated surgical field. Permacol was implanted as a bridge in 46.7%, as an underlay (intraperitoneal position) in 38.3% and as a sublay (retromuscolar position) in 15% of patients. Fascia closure was achieved in 53.3% of patients. The surgical site occurrence rate was 35% and the defect size significantly influenced the probability of post-operative complications. The long-term (2 year) hernia recurrence rate was 36.2%. This study represents the first large multi-centre Italian case series on Permacol implants in patients with a CAWD. Our data suggest that Permacol is a feasible strategy to repair a CAWD, with acceptable early complications and long-term (2 year) recurrence rates.


Assuntos
Parede Abdominal/cirurgia , Colágeno/administração & dosagem , Hérnia Ventral/cirurgia , Telas Cirúrgicas , Parede Abdominal/fisiopatologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Materiais Biocompatíveis/administração & dosagem , Feminino , Hérnia Ventral/fisiopatologia , Herniorrafia , Humanos , Itália/epidemiologia , Masculino , Pessoa de Meia-Idade , Complicações Pós-Operatórias/fisiopatologia , Próteses e Implantes , Suínos
14.
J Vis Exp ; (156)2020 02 10.
Artigo em Inglês | MEDLINE | ID: mdl-32090989

RESUMO

The use of biocompatible materials for circumferential esophageal reconstruction is a technically challenging task in rats and requires an optimal implant technique with nutritional support. Recently, there have been many attempts at esophageal tissue engineering, but the success rate has been limited due to difficulty in early epithelization in the special environment of peristalsis. Here, we developed an artificial esophagus that can improve the regeneration of the esophageal mucosa and muscle layers through a two-layered tubular scaffold, a mesenchymal stem cell-based bioreactor system, and a bypass feeding technique with modified gastrostomy. The scaffold is made of polyurethane (PU) nanofibers in a cylindrical shape with a three-dimensional (3D) printed polycaprolactone strand wrapped around the outer wall. Prior to transplantation, human-derived mesenchymal stem cells were seeded into the lumen of the scaffold, and bioreactor cultivation was performed to enhance cellular reactivity. We improved the graft survival rate by applying surgical anastomosis and covering the implanted prosthesis with a thyroid gland flap, followed by temporary nonoral gastrostomy feeding. These grafts were able to recapitulate the findings of initial epithelialization and muscle regeneration around the implanted sites, as demonstrated by histological analysis. In addition, increased elastin fibers and neovascularization were observed in the periphery of the graft. Therefore, this model presents a potential new technique for circumferential esophageal reconstruction.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Esôfago/cirurgia , Sobrevivência de Enxerto , Procedimentos Cirúrgicos Reconstrutivos/métodos , Engenharia Tecidual/métodos , Tecidos Suporte , Animais , Esôfago/fisiologia , Sobrevivência de Enxerto/efeitos dos fármacos , Sobrevivência de Enxerto/fisiologia , Humanos , Células-Tronco Mesenquimais/fisiologia , Nanofibras/administração & dosagem , Poliésteres/administração & dosagem , Ratos , Ratos Sprague-Dawley
15.
Nanotoxicology ; 14(5): 577-594, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-31928284

RESUMO

The successful translation of nanostructure-based bioimaging and/or drug delivery system needs extensive in vitro and in vivo studies on biocompatibility, biodistribution, clearance, and toxicity for its diagnostic applications. Herein, we have investigated the in vitro cyto-hemocompatibility, in vivo biodistribution, clearance, and toxicity in mice after systemic administration of GdF3 nanoparticles loaded PEGylated mesoporous carbon capsule (GdF3-PMCC)-based theranostic system. In vitro cyto-hemocompatibility study showed a very good biocompatibility up to concentration of 500 µg/ml. Biodistribution studies carried out from 1 h to 8 days showed that GdF3-PMCC was found in major organs, such as liver, kidney, spleen, and muscle till 4th day and it was negligible in any tissue after 8th day. The clearance study was carried out for a period of 8 days and it was observed that the urinary system is the main route of excretion of GdF3-PMCC. The tissue toxicity study was done for 15 days and histopathological analysis indicated that the GdF3-PMCC based theranostic system does not have any adverse effect in tissues. Thus, PMCCs are nontoxic and can be applied as theranostic agents in contrast to the other carbon-based systems (PEGylated carbon nanotubes and PEGylated graphene oxide) which showed significant toxicity.


Assuntos
Antineoplásicos/administração & dosagem , Materiais Biocompatíveis/química , Carbono/química , Portadores de Fármacos/química , Compostos de Flúor/administração & dosagem , Gadolínio/química , Nanopartículas/administração & dosagem , Polietilenoglicóis/química , Nanomedicina Teranóstica , Animais , Antineoplásicos/farmacocinética , Antineoplásicos/farmacologia , Antineoplásicos/toxicidade , Apoptose/efeitos dos fármacos , Materiais Biocompatíveis/administração & dosagem , Coagulação Sanguínea/efeitos dos fármacos , Cápsulas , Carbono/administração & dosagem , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Portadores de Fármacos/administração & dosagem , Sistemas de Liberação de Medicamentos , Compostos de Flúor/farmacocinética , Compostos de Flúor/toxicidade , Gadolínio/administração & dosagem , Gadolínio/efeitos adversos , Gadolínio/farmacocinética , Gadolínio/toxicidade , Humanos , Rim/efeitos dos fármacos , Rim/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Taxa de Depuração Metabólica , Camundongos , Músculos/efeitos dos fármacos , Músculos/metabolismo , Nanopartículas/química , Polietilenoglicóis/administração & dosagem , Espécies Reativas de Oxigênio , Baço/efeitos dos fármacos , Baço/metabolismo , Distribuição Tecidual
18.
Nat Rev Urol ; 17(3): 151-161, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31974507

RESUMO

Stress urinary incontinence (SUI) is a common and bothersome condition. Anti-incontinence surgery has high cure rates, but concerns about mesh tapes have resulted in the resurgence of surgical procedures that involve increased abdominopelvic dissection and morbidity. Injection therapy with urethral bulking agents or stem cell formulations have been developed as minimally invasive alternatives. Many synthetic and biological bulking agents have been trialled, but several have been discontinued owing to safety concerns. The use of Macroplastique and Contigen has the largest evidence base, but, overall, success rates seem to be similar between the various agents and positive outcomes are poorly sustained for more than 6 months. Furthermore, subjective cure rates, although initially high, also deteriorate over time. The available data consistently demonstrate manifestly poorer outcomes for injection therapies than for surgery. Stem cell treatments are thought to functionally regenerate the urethral sphincter in patients with suspected intrinsic sphincter deficiency. Autologous adipose and muscle-derived stem cells seem to be the intuitive cell source, as they are comparatively abundant, can be harvested and cause minimal donor site morbidity. To date, only a few small clinical studies have been reported and most data are derived from animal models. The success rates of stem cell injection therapies seem to be comparable with those of bulking agents.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Doenças Uretrais/terapia , Incontinência Urinária por Estresse/terapia , Humanos , Injeções Intralesionais , Medicina Regenerativa/métodos , Transplante de Células-Tronco
19.
Colloids Surf B Biointerfaces ; 188: 110767, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31923775

RESUMO

Advancement in nanotechnology has brought abundant number of products and materials in multiple fields including biomedicine owing to their unique physico-chemical properties. This further necessitates toxicity assessment of nanoparticles (NPs) before they are employed for product fabrication, medicinal, environmental or industrial purposes. Zinc oxide nanoparticles (ZnONPs) belong to the category of metal oxide NPs and hold quite a lot of possibilities to be applied in aforementioned scenarios. Present study addresses the probable outcomes of bio-nano interaction of ZnONPs with healthy adult Wistar rats. Sphere head shaped ZnONPs were synthesized via wet chemical method. Physico-chemical characterization was performed using number of sophisticated techniques including HR-TEM, Zeta potential analysis, TGA and XRD. Size of the particles was found to be 43 nm and ensured homogenous distribution with high purity. For in vivo studies, as synthesized NPs were administered into rats via intravenous (i.v.) and intraperitoneal (i.p.) routes. Animals were sacrificed on 3rd, 14th and 21st day of exposure. Metabolically relevant tissues like brain, liver, kidneys and spleen were isolated and analyzed for different parameters like gross pathology, haematology, neurotoxicity, target organ toxicity, immunotoxicity etc. Results suggests that ZnONPs did not elicit significant toxic responses in rat except a few anomalies with histology, ion content and antioxidant system within liver; thereby confirming potent hepatotoxicity. Hence the study recommends adopting surface functionalization strategies for reducing toxic response of ZnONPs during various application rationales.


Assuntos
Antioxidantes/toxicidade , Materiais Biocompatíveis/toxicidade , Fígado/efeitos dos fármacos , Nanopartículas/toxicidade , Óxido de Zinco/toxicidade , Animais , Antioxidantes/administração & dosagem , Antioxidantes/química , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/química , Peso Corporal/efeitos dos fármacos , Injeções Intraperitoneais , Injeções Intravenosas , Fígado/patologia , Masculino , Nanopartículas/administração & dosagem , Nanopartículas/química , Tamanho do Órgão/efeitos dos fármacos , Tamanho da Partícula , Ratos , Ratos Wistar , Espectrofotometria Atômica , Propriedades de Superfície , Óxido de Zinco/administração & dosagem , Óxido de Zinco/química
20.
Am J Respir Cell Mol Biol ; 62(1): 14-22, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31513744

RESUMO

Maintaining the three-dimensional architecture and cellular complexity of lung tissue ex vivo can enable elucidation of the cellular and molecular pathways underlying chronic pulmonary diseases. Precision-cut lung slices (PCLS) are one human-lung model with the potential to support critical mechanistic studies and early drug discovery. However, many studies report short culture times of 7-10 days. Here, we systematically evaluated poly(ethylene glycol)-based hydrogel platforms for the encapsulation of PCLS. We demonstrated the ability to support ex vivo culture of embedded PCLS for at least 21 days compared with control PCLS floating in media. These customized hydrogels maintained PCLS architecture (no difference), viability (4.7-fold increase, P < 0.0001), and cellular phenotype as measured by SFTPC (1.8-fold increase, P < 0.0001) and vimentin expression (no change) compared with nonencapsulated controls. Collectively, these results demonstrate that hydrogel biomaterials support the extended culture times required to study chronic pulmonary diseases ex vivo using PCLS technology.


Assuntos
Materiais Biocompatíveis/administração & dosagem , Hidrogéis/administração & dosagem , Pulmão/patologia , Técnicas de Cultura de Órgãos/métodos , Animais , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Doença Pulmonar Obstrutiva Crônica/patologia
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