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1.
Cell Prolif ; 53(11): e12906, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33043500

RESUMO

OBJECTIVES: Silicate bioactive glass (BG) has been widely demonstrated to stimulate both of the hard and soft tissue regeneration, in which ion products released from BG play important roles. However, the mechanism by which ion products act on cells on cells is unclear. MATERIALS AND METHODS: Human umbilical vein endothelial cells and human bone marrow stromal cells were used in this study. Fluorescence recovery after photobleaching and generalized polarization was used to characterize changes in cell membrane fluidity. Migration, differentiation and apoptosis experiments were carried out. RNA and protein chip were detected. The signal cascade is simulated to evaluate the effect of increased cell membrane fluidity on signal transduction. RESULTS: We have demonstrated that ion products released from BG could effectively enhance cell membrane fluidity in a direct and physical way, and Si ions may play a major role. Bioactivities of BG ion products on cells, such as migration and differentiation, were regulated by membrane fluidity. Furthermore, we have proved that BG ion products could promote apoptosis of injured cells based on our conclusion that BG ion products increased membrane fluidity. CONCLUSIONS: This study proved that BG ion products could develop its bioactivity on cells by directly enhancing cell membrane fluidity and subsequently affected cell behaviours, which may provide an explanation for the general bioactivities of silicate material.


Assuntos
Materiais Biocompatíveis/metabolismo , Cerâmica/metabolismo , Células Endoteliais da Veia Umbilical Humana/citologia , Fluidez de Membrana , Células-Tronco Mesenquimais/citologia , Cátions Monovalentes/metabolismo , Diferenciação Celular , Linhagem Celular , Movimento Celular , Células Cultivadas , Células Endoteliais da Veia Umbilical Humana/metabolismo , Humanos , Íons/metabolismo , Células-Tronco Mesenquimais/metabolismo , Silício/metabolismo
2.
Int J Nanomedicine ; 15: 6097-6111, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32884266

RESUMO

The development of biomaterials, stem cells and bioactive factors has led to cartilage tissue engineering becoming a promising tactic to repair cartilage defects. Various polymer three-dimensional scaffolds that provide an extracellular matrix (ECM) mimicking environment play an important role in promoting cartilage regeneration. In addition, numerous growth factors have been found in the regenerative process. However, it has been elucidated that the uncontrolled delivery of these factors cannot fully exert regenerative potential and can also elicit undesired side effects. Considering the complexity of the ECM, neither scaffolds nor growth factors can independently obtain successful outcomes in cartilage tissue engineering. Therefore, collectively, an appropriate combination of growth factors and scaffolds have great potential to promote cartilage repair effectively; this approach has become an area of considerable interest in recent investigations. Of late, an increasing trend was observed in cartilage tissue engineering towards this combination to develop a controlled delivery system that provides adequate physical support for neo-cartilage formation and also enables spatiotemporally delivery of growth factors to precisely and fully exert their chondrogenic potential. This review will discuss the role of polymer scaffolds and various growth factors involved in cartilage tissue engineering. Several growth factor delivery strategies based on the polymer scaffolds will also be discussed, with examples from recent studies highlighting the importance of spatiotemporal strategies for the controlled delivery of single or multiple growth factors in cartilage tissue engineering applications.


Assuntos
Cartilagem , Peptídeos e Proteínas de Sinalização Intercelular/administração & dosagem , Polímeros/química , Engenharia Tecidual/métodos , Tecidos Suporte/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Cartilagem/citologia , Cartilagem/fisiologia , Condrogênese , Matriz Extracelular/metabolismo , Humanos , Peptídeos e Proteínas de Sinalização Intercelular/farmacologia , Células-Tronco
3.
Nature ; 584(7822): 535-546, 2020 08.
Artigo em Inglês | MEDLINE | ID: mdl-32848221

RESUMO

Substantial research over the past two decades has established that extracellular matrix (ECM) elasticity, or stiffness, affects fundamental cellular processes, including spreading, growth, proliferation, migration, differentiation and organoid formation. Linearly elastic polyacrylamide hydrogels and polydimethylsiloxane (PDMS) elastomers coated with ECM proteins are widely used to assess the role of stiffness, and results from such experiments are often assumed to reproduce the effect of the mechanical environment experienced by cells in vivo. However, tissues and ECMs are not linearly elastic materials-they exhibit far more complex mechanical behaviours, including viscoelasticity (a time-dependent response to loading or deformation), as well as mechanical plasticity and nonlinear elasticity. Here we review the complex mechanical behaviours of tissues and ECMs, discuss the effect of ECM viscoelasticity on cells, and describe the potential use of viscoelastic biomaterials in regenerative medicine. Recent work has revealed that matrix viscoelasticity regulates these same fundamental cell processes, and can promote behaviours that are not observed with elastic hydrogels in both two- and three-dimensional culture microenvironments. These findings have provided insights into cell-matrix interactions and how these interactions differentially modulate mechano-sensitive molecular pathways in cells. Moreover, these results suggest design guidelines for the next generation of biomaterials, with the goal of matching tissue and ECM mechanics for in vitro tissue models and applications in regenerative medicine.


Assuntos
Elasticidade , Matriz Extracelular/metabolismo , Substâncias Viscoelásticas , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Técnicas de Cultura de Células , Forma Celular , Matriz Extracelular/química , Humanos , Mecanotransdução Celular , Células-Tronco Mesenquimais/citologia , Modelos Biológicos , Medicina Regenerativa
4.
Beijing Da Xue Xue Bao Yi Xue Ban ; 52(3): 564-569, 2020 Jun 18.
Artigo em Chinês | MEDLINE | ID: mdl-32541993

RESUMO

OBJECTIVE: To study the biodegradation properties of multi-laminated small intestinal submucosa (mSIS) through in vitro and in vivo experiments, comparing with Bio-Gide, the most widely used collagen membrane in guided bone regeneration (GBR) technique, for the purpose of providing basis to investigate whether mSIS meets the requirements of GBR in dental clinics. METHODS: The degradation properties were evaluated in vitro and in vivo. In vitro degradation was performed using prepared collagenase solution. Morphology of mSIS and Bio-Gide in degradation solution were observed and the degradation rate was calculated at different time points. In in vivo experiments, nine New Zealand rabbits were used for subcutaneous implantation and were divided into three groups according to observation intervals. Six unconnected subcutaneous pouches were made on the back of each animal and were embedded with mSIS and Bio-Gide respectively. At the end of weeks 4, 8, and 12 after operation, gross observation and HE staining were used to evaluate the degree of degradation and histocompatibility. RESULTS: In vitro degradation experiments showed that mSIS membrane was completely degraded at the end of 12 days, while Bio-Gide was degraded at the end of 7 days. Besides, mSIS maintained its shape for longer time in the degradation solution than Bio-Gide, indicating that mSIS possessed longer degradation time, and had better ability to maintain space than Bio-Gide. In vivo biodegradation indicated that after 4 weeks of implantation, mSIS remained intact. Microscopic observation showed that collagen fibers were continuous with a few inflammatory cells that infiltrated around the membrane. Bio-Gide was basically intact and partially adhered with the surrounding tissues. HE staining showed that collagen fibers were partly fused with surrounding tissues with a small amount of inflammatory cells that infiltrated as well. Eight weeks after operation, mSIS was still intact, and was partly integrated with connective tissues, whereas Bio-Gide membrane was mostly broken and only a few residual fibers could be found under microscope. Only a small amount of mSIS debris could be observed 12 weeks after surgery, and Bio-Gide could hardly be found by naked eye and microscopic observation at the same time. CONCLUSION: In vitro degradation time of mSIS is longer than that of Bio-Gide, and the space-maintenance ability of mSIS is better. The in vivo biodegradation time of subcutaneous implantation of mSIS is about 12 weeks and Bio-Gide is about 8 weeks, both of which possess good biocompatibility.


Assuntos
Materiais Biocompatíveis/metabolismo , Animais , Regeneração Óssea , Tecido Conjuntivo , Mucosa Intestinal , Intestino Delgado , Membranas Artificiais , Coelhos
5.
J Nanobiotechnology ; 18(1): 59, 2020 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-32293461

RESUMO

BACKGROUND: Infectious diseases caused by multidrug-resistant (MDR) bacteria, especially MDR Gram-negative strains, have become a global public health challenge. Multifunctional nanomaterials for controlling MDR bacterial infections via eradication of planktonic bacteria and their biofilms are of great interest. RESULTS: In this study, we developed a multifunctional platform (TG-NO-B) with single NIR laser-triggered PTT and NO release for synergistic therapy against MDR Gram-negative bacteria and their biofilms. When located at the infected sites, TG-NO-B was able to selectively bind to the surfaces of Gram-negative bacterial cells and their biofilm matrix through covalent coupling between the BA groups of TG-NO-B and the bacterial LPS units, which could greatly improve the antibacterial efficiency, and reduce side damages to ambient normal tissues. Upon single NIR laser irradiation, TG-NO-B could generate hyperthermia and simultaneously release NO, which would synergistically disrupt bacterial cell membrane, further cause leakage and damage of intracellular components, and finally induce bacteria death. On one hand, the combination of NO and PTT could largely improve the antibacterial efficiency. On the other hand, the bacterial cell membrane damage could improve the permeability and sensitivity to heat, decrease the photothermal temperature and avoid damages caused by high temperature. Moreover, TG-NO-B could be effectively utilized for synergistic therapy against the in vivo infections of MDR Gram-negative bacteria and their biofilms and accelerate wound healing as well as exhibit excellent biocompatibility both in vitro and in vivo. CONCLUSIONS: Our study demonstrates that TG-NO-B can be considered as a promising alternative for treating infections caused by MDR Gram-negative bacteria and their biofilms.


Assuntos
Biofilmes/efeitos da radiação , Farmacorresistência Bacteriana Múltipla/efeitos da radiação , Bactérias Gram-Negativas/fisiologia , Raios Infravermelhos , Óxidos de Nitrogênio/metabolismo , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Biofilmes/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Infecções por Bactérias Gram-Negativas/patologia , Infecções por Bactérias Gram-Negativas/terapia , Infecções por Bactérias Gram-Negativas/veterinária , Grafite/química , Hemólise/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Testes de Sensibilidade Microbiana , Células NIH 3T3 , Nanoestruturas/química , Nanoestruturas/toxicidade , Fototerapia , Temperatura , Distribuição Tecidual , Cicatrização/efeitos dos fármacos , Cicatrização/efeitos da radiação
6.
Proc Natl Acad Sci U S A ; 117(6): 2770-2778, 2020 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-31988132

RESUMO

Organelle-specific nanocarriers (NCs) are highly sought after for delivering therapeutic agents into the cell nucleus. This necessitates nucleocytoplasmic transport (NCT) to bypass nuclear pore complexes (NPCs). However, little is known as to how comparably large NCs infiltrate this vital intracellular barrier to enter the nuclear interior. Here, we developed nuclear localization signal (NLS)-conjugated polymersome nanocarriers (NLS-NCs) and studied the NCT mechanism underlying their selective nuclear uptake. Detailed chemical, biophysical, and cellular analyses show that karyopherin receptors are required to authenticate, bind, and escort NLS-NCs through NPCs while Ran guanosine triphosphate (RanGTP) promotes their release from NPCs into the nuclear interior. Ultrastructural analysis by regressive staining transmission electron microscopy further resolves the NLS-NCs on transit in NPCs and inside the nucleus. By elucidating their ability to utilize NCT, these findings demonstrate the efficacy of polymersomes to deliver encapsulated payloads directly into cell nuclei.


Assuntos
Núcleo Celular/metabolismo , Nanopartículas/química , Polímeros/química , Transporte Ativo do Núcleo Celular , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Núcleo Celular/genética , Sistemas de Liberação de Medicamentos , Guanosina Trifosfato/metabolismo , Células HeLa , Humanos , Carioferinas , Nanopartículas/metabolismo , Sinais de Localização Nuclear/química , Sinais de Localização Nuclear/metabolismo , Poro Nuclear/metabolismo , Polímeros/metabolismo
7.
ACS Appl Mater Interfaces ; 12(5): 5437-5446, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31917532

RESUMO

A major problem of current biomedical implants is the bacterial colonization and subsequent biofilm formation, which seriously affects their functioning and can lead to serious post-surgical complications. Intensive efforts have been directed toward the development of novel technologies that can prevent bacterial colonization while requiring minimal antibiotics doses. To this end, biocompatible materials with intrinsic antifouling capabilities are in high demand. Silk fibroin, widely employed in biotechnology, represents an interesting candidate. Here, we employ a soft-lithography approach to realize micro- and nanostructured silk fibroin substrates, with different geometries. We show that patterned silk film substrates support mammal cells (HEK-293) adhesion and proliferation, and at the same time, they intrinsically display remarkable antifouling properties. We employ Escherichia coli as representative Gram-negative bacteria, and we observe an up to 66% decrease in the number of bacteria that adhere to patterned silk surfaces as compared to control, flat silk samples. The mechanism leading to the inhibition of biofilm formation critically depends on the microstructure geometry, involving both a steric and a hydrophobic effect. We also couple silk fibroin patterned films to a biocompatible, optically responsive organic semiconductor, and we verify that the antifouling properties are very well preserved. The technology described here is of interest for the next generation of biomedical implants, involving the use of materials with enhanced antibacterial capability, easy processability, high biocompatibility, and prompt availability for coupling with photoimaging and photodetection techniques.


Assuntos
Incrustação Biológica/prevenção & controle , Nanoestruturas/química , Seda/química , Aderência Bacteriana/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Biofilmes/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Escherichia coli/fisiologia , Células HEK293 , Humanos , Interações Hidrofóbicas e Hidrofílicas , Propriedades de Superfície
8.
Biomed Tech (Berl) ; 65(1): 107-111, 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31348752

RESUMO

Osteocytes are of high importance in bone metabolism as they orchestrate bone remodeling, react to mechanosensory stimuli and have endocrine functions. In vitro investigations with osteocytes are therefore of high relevance for biomaterial and drug testing. The application of primary human cells instead of rodent osteocyte cell lines like MLOY4 and IDG SW3 is desirable but provides the challenge of isolating these cells, which are deeply embedded into the mineralized bone matrix. The present study describes an improved protocol for the isolation of human primary osteocytes. In contrast to an already established protocol, resting steps between the demineralization /digestion steps of the bone particles considerably improved the yield of osteocytes. Real-time polymerase chain reaction (PCR) analysis revealed the expression of typical osteocyte markers like osteocalcin, E11/podoplanin and dentin matrix protein 1 (DMP-1).


Assuntos
Materiais Biocompatíveis/metabolismo , Biomarcadores/química , Osso e Ossos/metabolismo , Proteínas da Matriz Extracelular/metabolismo , Osteócitos/citologia , Fosfoproteínas/metabolismo , Animais , Biomarcadores/metabolismo , Linhagem Celular , Proteínas da Matriz Extracelular/química , Humanos , Osteócitos/metabolismo , Osteócitos/fisiologia , Fosfoproteínas/química
9.
Chem Commun (Camb) ; 56(3): 399-402, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31820751

RESUMO

A combinatorial approach using a one-bead-one-compound method and a screening based on a SOD-activity assay was set up for the discovery of an efficient peptidyl copper complex. The complex exhibited good stability constants, suitable redox potentials and excellent intrinsic activity. This complex was further assayed in cells for its antioxidant properties and showed beneficial effects when cells were subjected to oxidative stress.


Assuntos
Materiais Biocompatíveis/metabolismo , Cobre/química , Peptídeos/química , Sequência de Aminoácidos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Colo/citologia , Colo/efeitos dos fármacos , Colo/metabolismo , Cobre/metabolismo , Células HT29 , Humanos , Interleucina-8/metabolismo , Lipopolissacarídeos/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Peptídeos/metabolismo , Superóxido Dismutase/metabolismo
10.
J Colloid Interface Sci ; 563: 336-346, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31887697

RESUMO

Polymer graphitic carbon nitride (g-C3N4) materials have attracted growing interest owing to their impressive applicability in photocatalysis and optoelectronic devices. However, further applications of g-C3N4 materials are greatly restricted by their chemical inertness and insolubility in most solvents. Regarding the rising prospect of g-C3N4 nanosheets in the biomedicalfield, high solubility and biocompatibility are required for the further development of g-C3N4 materials. In this study, a simple one-step thermal polymerization method was designed to prepare fast-soluble mesoporous g-C3N4 nanosheets by using NH4HSO4 as the critical adjuvant. The products, especially the optimal g-C3N4 NSs-4, showed impressive solubility, biocompatibility and partial biodegradability. The enriched surface hydrophilic groups (-NH2 and -OH) may contribute to improving the solubility of g-C3N4 nanosheets, while the partial biodegradability can be ascribed to the presence of the disulfide bond in the g-C3N4 framework. In this system, the NH4HSO4 adjuvant acted not only as O and S sources, but also as a bubbling agent that endows the g-C3N4 a porous structure with greatly enlarged specific surface area and high separation efficiency of photogenerated electron-hole pairs. These integrative positive factors also greatly contributed to the photocatalytic activity of the g-C3N4 nanosheets. This facile, economic and general fabrication strategy for mesoporous, fast-soluble and biocompatible g-C3N4 with superior visible-light photocatalytic activity is promising in environmental, energy and biomedical fields.


Assuntos
Materiais Biocompatíveis/metabolismo , Grafite/metabolismo , Compostos de Nitrogênio/metabolismo , Animais , Materiais Biocompatíveis/química , Catálise , Linhagem Celular Tumoral , Grafite/química , Camundongos , Compostos de Nitrogênio/química , Tamanho da Partícula , Processos Fotoquímicos , Propriedades de Superfície
11.
Eur J Vasc Endovasc Surg ; 59(4): 643-652, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-31874809

RESUMO

OBJECTIVE: Biodegradable materials for in situ vascular tissue engineering could meet the increasing clinical demand for sufficient synthetic small diameter vascular substitutes in aortocoronary bypass and peripheral vascular surgery. The aim of this study was to design a new degradable thermoplastic polycarbonate urethane (dPCU) with improved biocompatibility and optimal biomechanical properties. Electrospun conduits made from dPCU were evaluated in short and long term follow up and compared with expanded polytetrafluoroethylene (ePTFE) controls. METHODS: Both conduits were investigated prior to implantation to assess their biocompatibility and inflammatory potential via real time polymerase chain reaction using a macrophage culture. dPCU grafts (n = 28) and ePTFE controls (n = 28) were then implanted into the infrarenal abdominal aorta of Sprague-Dawley rats. After seven days, one, six, and 12 months, grafts were analysed by histology and immunohistochemistry (IHC) and assessed biomechanically. RESULTS: Anti-inflammatory signalling was upregulated in dPCU conduits and increased significantly over time in vitro. dPCU and ePTFE grafts offered excellent long and short term patency rates (92.9% in both groups at 12 months) in the rat model without dilatation or aneurysm formation. In comparison to ePTFE, dPCU grafts showed transmural ingrowth of vascular specific cells resulting in a structured neovessel formation around the graft. The graft material was slowly reduced, while the compliance of the neovessel increased over time. CONCLUSION: The newly designed dPCU grafts have the potential to be safely applied for in situ vascular tissue engineering applications. The degradable substitutes showed good in vivo performance and revealed desirable characteristics such as biomechanical stability, non-thrombogenicity, and minimal inflammatory response after long term implantation.


Assuntos
Implantes Absorvíveis , Nanofibras/uso terapêutico , Cimento de Policarboxilato/farmacologia , Tempo , Implantes Absorvíveis/efeitos adversos , Animais , Materiais Biocompatíveis/metabolismo , Implante de Prótese Vascular , Politetrafluoretileno/farmacologia , Ratos Sprague-Dawley , Reimplante/métodos , Uretana/farmacologia , Grau de Desobstrução Vascular/efeitos dos fármacos
12.
Mater Sci Eng C Mater Biol Appl ; 107: 110300, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761223

RESUMO

Application of restructured collagen-based biomaterials is generally restricted by their poor mechanical properties, which ideally must be close to those of a tissue being repaired. Here, we present an approach to the formation of a robust biomaterial using laser-induced curing of a photosensitive star-shaped polylactide. The created collagen-based structures demonstrated an increase in the Young's modulus by more than an order of magnitude with introduction of reinforcing patterns (from 0.15 ±â€¯0.02 MPa for the untreated collagen to 51.2 ±â€¯5.6 MPa for the reinforced collagen). It was shown that the geometrical configuration of the created reinforcing pattern affected the scaffold's mechanical properties only in the case of a relatively high laser radiation power density, when the effect of accumulated thermomechanical stresses in the photocured regions was significant. Photo-crosslinking of polylactide did not compromise the scaffold's cytotoxicity and provided fluorescent regions in the collagen matrix, that create a potential for noninvasive monitoring of such materials' biodegradation kinetics in vivo.


Assuntos
Materiais Biocompatíveis , Colágeno , Poliésteres , Tecidos Suporte/química , Células 3T3 , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Colágeno/química , Colágeno/metabolismo , Módulo de Elasticidade , Teste de Materiais , Camundongos , Processos Fotoquímicos , Poliésteres/química , Poliésteres/metabolismo , Riboflavina/química
13.
Mater Sci Eng C Mater Biol Appl ; 107: 110243, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761159

RESUMO

In this work, dextran based membranes with potential to be used as implantable devices in Tissue Engineering and Regenerative Medicine (TERM) were prepared by a straightforward strategy. Briefly, two polymers approved by the Food and Drug Administration, viz. dextran and poly(ε-caprolactone) (PCL) were functionalized with methacrylate moieties, and subjected to photocrosslinking. Employing different weight ratios of each polymer in the formulations allowed to obtain transparent membranes with tunable physicochemical properties and low adverse host tissue response. Independently of the material, all formulations have shown to be thermally stable up to 300 °C whilst variations in the polymer ratio resulted in membranes with different glass transition temperatures (Tg) and flexibility. The swelling capacity ranged from 50% to 200%. On the other hand, in vitro hydrolytic degradation did not show to be material-dependent and all membranes maintained their structural integrity for more than 30 days, losing only 8-12% of their initial weight. Preliminary in vitro biological tests did not show any cytotoxic effect on seeded human dental pulp stem cells (hDPSCs), suggesting that, in general, all membranes are capable of supporting cell adhesion and viability. The in vivo biocompatibility of membranes implanted subcutaneously in rats' dorsum indicate that M100/0 (100%wt dextran) and M25/75 (25 %wt dextran) formulations can be classified as "slight-irritant" and "non-irritant", respectively. From the histological analysis performed on the main tissue organs it was not possible to detect any signs of fibrosis or necrosis thereby excluding the presence of toxic degradation by-products deposited or accumulated in these tissues. In combination, these results suggest that the newly developed formulations hold great potential as engineered devices for biomedical applications, where the biological response of cells and tissues are greatly dependent on the physical and chemical cues provided by the substrate.


Assuntos
Materiais Biocompatíveis/química , Dextranos/química , Membranas Artificiais , Animais , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Cálcio/metabolismo , Adesão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Polpa Dentária/citologia , Humanos , Masculino , Metacrilatos/química , Poliésteres/química , Próteses e Implantes , Ratos , Ratos Sprague-Dawley , Células-Tronco/citologia , Células-Tronco/metabolismo , Tela Subcutânea/metabolismo , Tela Subcutânea/patologia
14.
Mater Sci Eng C Mater Biol Appl ; 107: 110254, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31761216

RESUMO

Polymorphous light eruption (PLE) is one of the acquired idiopathic photodermatosis mainly induced by immoderate UV radiation. In order to realize UV protection and medicine administration simultaneously for polymorphous light eruption protection and therapy, Acetyl-11-keto-ß-boswellic acid (AKBA) loaded Zinc Oxide (ZnO) nanoparticles of which drug release behavior is UV-controlled has been successfully synthesized. Such nanoparticles can not only reflect UV but also transfer the energy to release AKBA which presents an excellent antioxidant and anti-inflammatory effects. In addition, they are biocompatible to HaCaT cells. As a result, they have a great potential in combining UV protection and medicine administration simultaneously for PLE protection and therapy.


Assuntos
Nanopartículas/química , Triterpenos/química , Raios Ultravioleta , Óxido de Zinco/química , Anti-Inflamatórios/química , Anti-Inflamatórios/metabolismo , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Ciclo-Oxigenase 2/genética , Ciclo-Oxigenase 2/metabolismo , Liberação Controlada de Fármacos , Humanos , Queratinócitos/citologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Nanopartículas/toxicidade , Transtornos de Fotossensibilidade/patologia , Transtornos de Fotossensibilidade/prevenção & controle , Espécies Reativas de Oxigênio , Triterpenos/metabolismo , Triterpenos/farmacologia
15.
J Colloid Interface Sci ; 563: 92-103, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31869588

RESUMO

HYPOTHESIS: The treatment of bone fractures still represents a challenging clinical issue when complications due to impaired bone remodelling (i.e. osteoporosis) or infections occur. These clinical needs still require a radical improvement of the existing therapeutic approach through the design of advanced biomaterials combining the ability to promote bone regeneration with anti-adhesive properties able to minimise unspecific biomolecules adsorption and bacterial adhesion. Strontium-containing mesoporous bioactive glasses (Sr-MBG), which are able to exert a pro-osteogenic effect by releasing Sr2+ ions, have been successfully functionalised to provide mixed-charge (NH3⊕/COO⊝) surface groups with anti-adhesive abilities. EXPERIMENTS: Sr-MBG have been post-synthesis modified by co-grafting hydrolysable short chain silanes containing amino (aminopropylsilanetriol) and carboxylate (carboxyethylsilanetriol) moieties to achieve a zwitterionic zero-charge surface. The final system was then characterised in terms of textural-structural properties, bioactivity, cytotoxicity, pro-osteogenic and anti-adhesive capabilities. FINDINGS: After zwitterionization the in vitro bioactivity was maintained, as well as the ability to release Sr2+ ions which are capable of inducing a mineralization process. Irrespective of their size, Sr-MBG particles did not exhibit any cytotoxicity in pre-osteoblastic MC3T3-E1 up to the concentration of 75 µg/mL. Finally, the zwitterionic Sr-MBGs showed a significant reduction of serum protein adhesion with respect to the pristine ones. These results open promising future expectations in the design of nanosystems which combine pro-osteogenic and anti-adhesive properties.


Assuntos
Materiais Biocompatíveis/metabolismo , Regeneração Óssea , Osso e Ossos/metabolismo , Estrôncio/metabolismo , Animais , Materiais Biocompatíveis/química , Osso e Ossos/química , Vidro/química , Humanos , Tamanho da Partícula , Porosidade , Estrôncio/química , Propriedades de Superfície
16.
Sensors (Basel) ; 20(1)2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31861836

RESUMO

Human NAD(P)H:quinone oxidoreductase 1 (hNQO1) is overexpressed in cancer cells and associated with the drug resistance factor of cancer. The objective of this work is the development of fluorescent probes for the efficient detection of hNQO1 activity in cancer cells, which can be employed for the cancer diagnosis and therapeutic agent development. Herein, we report naphthalimide-based fluorescent probes 1 and 2 that can detect hNQO1. For hNQO1 activity, the probes showed a significant fluorescence increase at 540 nm. In addition, probe 1, the naphthalimide containing a triphenylphosphonium salt, showed an enhanced enzyme efficiency and rapid detection under a physiological condition. The detection ability of probe 1 was superior to that of other previously reported probes. Moreover, probe 1 was less cytotoxic during the cancer cell imaging and readily provided a strong fluorescence in hNQO1-overexpressed cancer cells (A549). We proposed that probe 1 can be used to detect hNQO1 expression in live cells and it will be applied to develop the diagnosis and customized treatment of hNQO1-related disease.


Assuntos
Materiais Biocompatíveis/metabolismo , Corantes Fluorescentes/metabolismo , NAD(P)H Desidrogenase (Quinona)/metabolismo , Naftalimidas/metabolismo , Células A549 , Materiais Biocompatíveis/química , Linhagem Celular Tumoral , Ensaios Enzimáticos/métodos , Corantes Fluorescentes/química , Humanos , Concentração de Íons de Hidrogênio , Microscopia Confocal , NAD/química , NAD(P)H Desidrogenase (Quinona)/antagonistas & inibidores , NAD(P)H Desidrogenase (Quinona)/genética , Naftalimidas/química , Espectrometria de Fluorescência
17.
Adv Exp Med Biol ; 1174: 187-221, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31713200

RESUMO

The extraordinary mechanical properties of spider silk fibers result from the interplay of composition, structure and self-assembly of spider silk proteins (spidroins). Genetic approaches enabled the biotechnological production of recombinant spidroins which have been employed to unravel the self-assembly and spinning process. Various processing conditions allowed to explore non-natural morphologies including nanofibrils, particles, capsules, hydrogels, films or foams. Recombinant spider silk proteins and materials made thereof can be utilized for biomedical applications, such as drug delivery, tissue engineering or 3D-biomanufacturing.


Assuntos
Materiais Biocompatíveis , Fibroínas , Nanoestruturas , Proteínas Recombinantes , Aranhas , Animais , Materiais Biocompatíveis/metabolismo , Biotecnologia , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Seda/química , Aranhas/química
18.
Artif Cells Nanomed Biotechnol ; 47(1): 4211-4221, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31713444

RESUMO

At present, cancer is the first cause of death for humans, but early detection and treatment can help improve prognoses and reduce mortality. However, further development of carrier-assistant drug delivery systems (DDSs) is retarded by the aspects such as the low drug-carrying capacity, carrier-induced toxicity and immunogenicity, complex synthesis manipulation. The development of nanoscale drug delivery systems (NDDS) have been rapidly developed to address these issues. In this article, we used PLGA-PEG with good biocompatibility to encapsulate Fe3O4 nanoparticles (a magnetic resonance imaging contrast agent) and DOX (an antitumour drug) via the emulsion-solvent evaporation method, aimed at achieving a dual function of the early detection and the treatment of mammary cancer. The results showed that the Fe3O4/DOX/PLGA-PEG nanoparticles had a relatively uniform size, a high carrier rate of Fe3O4 and high encapsulation efficiency of DOX, and a relatively high activity of released DOX within 120 h. In addition, in vitro studies showed that the Fe3O4/DOX/PLGA-PEG nanoparticles were cytocompatibility in NIH 3T3 fibroblast cells culture study while had a special effect on destroying human breast cancer MCF-7 cells compared with pure DOX solution. In vitro studies revealed that the Fe3O4/DOX/PLGA-PEG enabled enhanced T2 contrast magnetic resonance. Overall, our multifunctional Fe3O4/DOX/PLGA-PEG nanoparticles, composed of biocompatible substances and therapeutic/imaging materials, have great potential for the early detection of cancer and accurate drug delivery via the dynamic monitoring using MRI.


Assuntos
Materiais Biocompatíveis/química , Doxorrubicina/química , Doxorrubicina/uso terapêutico , Portadores de Fármacos/química , Detecção Precoce de Câncer , Nanopartículas de Magnetita/química , Poliésteres/química , Polietilenoglicóis/química , Células 3T3 , Animais , Materiais Biocompatíveis/metabolismo , Transporte Biológico , Cápsulas , Portadores de Fármacos/metabolismo , Liberação Controlada de Fármacos , Imagem por Ressonância Magnética , Camundongos , Tamanho da Partícula , Solventes/química
19.
Artif Cells Nanomed Biotechnol ; 47(1): 4222-4233, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31713452

RESUMO

Gold nanoparticles (AuNPs), as a kind of inorganic nanoparticle, have been gradually recognized as one of the most promising nanomaterials, which is attributed to their unique optical, electronic, sensing and biochemical characteristics. Due to such unique characteristics, AuNPs have been widely applied in biomedical fields such as diagnosis, biosensing and drug delivery. Except for their use in cancer treatment alone with their photothermal ablation of solid tumours, when used with anticancer drugs, AuNPs can exert a dual role in treating cancer. With the advantages of protecting drugs from degradation and leakage in the physiological environment, tuneable modification in size, surface and shape, and biocompatibility, AuNPs can be used as promising drug carriers in anticancer drug design. However, there are still many aspects that need to be improved during the usage of drug carriers in pharmacology including the following aspects: prolongation in the plasma, enhancement in targeting accumulation, improvement in cellular uptake and the control of intracellular release. AuNPs are important drug carriers.


Assuntos
Portadores de Fármacos/química , Ouro/química , Nanopartículas Metálicas/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Portadores de Fármacos/metabolismo , Ouro/metabolismo , Humanos , Ligantes
20.
Biomater Sci ; 7(12): 4984-4989, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31667486

RESUMO

To exploit the usability of Digital Light Processing (DLP) in regenerative medicine, biodegradable, mechanically customizable and well-defined polyester urethane acrylate resins were synthesized based on poly(caprolactone-co-trimethlenecarbonate). By controlling the monomer ratio, the resultant fabricated constructs showed tunable mechanical properties, degradation and attached hMSC morphologies.


Assuntos
Implantes Absorvíveis , Resinas Acrílicas/química , Materiais Biocompatíveis/química , Caproatos/química , Lactonas/química , Luz , Polímeros/química , Poliuretanos/química , Engenharia Tecidual , Materiais Biocompatíveis/metabolismo , Teste de Materiais , Imagem Óptica , Polímeros/metabolismo , Tecidos Suporte/química
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