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1.
Georgian Med News ; (294): 145-150, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31687968

RESUMO

The main aim is to find out the mechanisms of reparative regeneration of bone tissue in artificially created transverse mandibular defects in rats when applying osteoplastic materials based on hydroxyapatite and polylactide with different component percentage. Experimental studies were carried out on 114 mature laboratory white male 180-220 g rats that were divided into groups: control group - animals whose bone defect was filled only with a blood clot; 1st experimental group - the entire defect volume was densely filled with a block with the ratio of component parts, hydroxyapatite 80% + polylactide 20%; 2nd experimental group was similar, but with the ratio of 50% + 50%. Morphological and morphometric research methods were carried out after the removed jaws had been decalcized in a 10% nitric acid solution. An x-ray study was performed using an Intra digital radiograph (Planmeca, Finland). Statistical analysis of the results was carried out according to the standard method of descriptive statistics using the software package "StatSoftStatistica 10". The use of osteoplastic material with a longer period of its biodegradation contributes to filling the defect with bone tissue, but slows down the processes of the final formation of a mature secondary bone scar, yet allows even with hypercorrection to restore the anatomical form of the lost area. Therefore, the use of this material is recommended for a defect in the alveolar ridge, mainly in extraction sockets, when one needs to avoid atrophy and to create conditions for prosthetic treatment. Osteoimplant with a faster biodegradation period allows structuring collagen fibers in the regenerate in the early stages of healing, which optimizes the formation of an organic bone matrix and promotes its faster mineralization. Therefore, such bone substitutes can be better used for insignificant defects or when the restoration of the anatomical form is not essential, mainly for defects of the body and mandibular branches.


Assuntos
Perda do Osso Alveolar/tratamento farmacológico , Materiais Biocompatíveis/uso terapêutico , Regeneração Óssea/efeitos dos fármacos , Substitutos Ósseos/farmacologia , Mandíbula/patologia , Poliésteres/farmacologia , Perda do Osso Alveolar/cirurgia , Animais , Substitutos Ósseos/uso terapêutico , Durapatita , Masculino , Mandíbula/cirurgia , Ratos , Cicatrização
2.
J Laparoendosc Adv Surg Tech A ; 29(11): 1446-1450, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31539310

RESUMO

Introduction: Symptomatic paraesophageal hernia (PEH) is an indication for surgical repair. Laparoscopic suture repair has high recurrence rates. Many surgeons prefer mesh repair to reduce PEH recurrence. Several types of mesh, synthetic and biological, are in use. Synthetic mesh has a risk of erosion and stricture, hence we preferred biological mesh repair. Our aim in this study is to assess medium-term outcomes of PEH repair with the use of biological mesh reinforcement over the cruroplasty. We also aimed to correlate clinical recurrences with radiological recurrences. Materials and Methods: This is a retrospective study of 154 consecutive patients from a single centre who underwent a standardized laparoscopic suture repair of the hiatus reinforced with an on-lay patch of Surgisis (porcine small intestine submucosa) and fundoplication. The mean age of the patients was 65 years. All patients were called for regular clinical follow-up and a barium study. Modified GERD-HRQL symptom severity instrument was used to assess postoperative symptoms and satisfaction. Results: The mean follow-up for barium swallow and clinical assessment were 28.42 ± 21.2 and 33.69 ± 23.46 months. The mean patient satisfaction score after surgery was 4.43 ± 1.09 (0-5). Follow-up barium swallow was performed in 122 (79.22%), 87 (56.49%) patients completed clinical follow-up questionnaire, and 77 (50%) had both. Symptomatic recurrence was noted in 25 (28.73%), recurrence on barium swallow 25(20.4%), and 10 (12.98%) had both. The reoperation rate was 3.25%. Mann-Whitney U test showed no statistical significance in reflux-related score between radiological recurrence group compared with no radiological recurrence (P = .06). Conclusions: Biological mesh repair of PEH is safe and well accepted by patients. There is significantly high PEH recurrence rate in long-term follow-up, even with mesh repair. Majority of these recurrences are small, asymptomatic, and the reoperation rate is very low.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Colágeno/uso terapêutico , Hérnia Hiatal/cirurgia , Herniorrafia/métodos , Telas Cirúrgicas , Idoso , Animais , Feminino , Seguimentos , Fundoplicatura , Refluxo Gastroesofágico/etiologia , Refluxo Gastroesofágico/cirurgia , Hérnia Hiatal/complicações , Hérnia Hiatal/diagnóstico por imagem , Humanos , Laparoscopia , Masculino , Pessoa de Meia-Idade , Radiografia , Recidiva , Reoperação , Estudos Retrospectivos , Inquéritos e Questionários , Suturas , Suínos , Resultado do Tratamento
3.
J Photochem Photobiol B ; 199: 111583, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31472461

RESUMO

Copper chalcogenides have been exhibited to be an encouraging photothermal operator because of their great photothermal transformation proficiency, engineered effortlessness, and ease. Notwithstanding, the hydrophobic and low biocompatibility attributes related with their manufactured procedures hamper broadly natural applications. An elective methodology for improve hydrophilic nature and biocompatibility to coating into the copper-based chalcogenide nanostructures containing core shell silica materials. In this manuscript, the level headed planning configuration results in effective covering silica nanostructures onto the synthesized Cu9S5 to form Cu9S5@MS core-shell nanostructures. The structural formation and nanostructures of prepared nanomaterials with core shell structure were confirmed via analysis of transmission microscopic and particles distribution investigates, which infers that Cu9S5@MS has been organized by nano level with high stability. Also, the formation of Cu9S5@MS was confirmed by UV-Visible and X-ray techniques. As-prepared Cu9S5@MS nanovesicles display good biocompatibility, and are successfully utilized for photothermal removal of disease cells and NIR therapy. Additionally, the mode of cell death in esophageal squamous carcinoma cells were monitored various staining techniques (AO and EB, nuclear staining and flowcytometry). Further, we evaluated by the human esophageal squamous cancer cell lines to observe cell cycle arrest ability. Significantly, we demonstrate the combination of photothermal and chemotherapeutic techniques through the prepared nanovesicles exhibits outstanding impacts in the treatment of esophageal cancer therapies in vitro and in vivo.


Assuntos
Antineoplásicos/química , Cobre/química , Carcinoma de Células Escamosas do Esôfago/terapia , Nanopartículas Metálicas/química , Fototerapia , Sulfetos/química , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/uso terapêutico , Linhagem Celular Tumoral , Carcinoma de Células Escamosas do Esôfago/enfermagem , Humanos , Fototerapia/métodos , Dióxido de Silício/química
4.
Rev. Asoc. Odontol. Argent ; 107(3): 110-115, jul.-sept. 2019.
Artigo em Espanhol | LILACS | ID: biblio-1048552

RESUMO

Este trabajo pretende actualizar los conocimientos acerca de los diversos materiales utilizados en las terapias pulpares (tanto en dientes primarios como en permanentes) que buscan una respuesta reparativa cada vez más conservadora, biológica y sustentable. Se trata de un recorrido por el uso de agentes como el agregado de trióxido mineral (MTA), el láser, Biodentine® y los concentrados de plasma rico en plaquetas, con sus características, sus posibles aplicaciones y su eficacia, evaluadas clínica y radiográficamente en múltiples trabajos de investigación, informes de casos, estudios comparativos (in vitro e in vivo) y ensayos experimentales en animales que documentan sus resultados (AU)


This literature review aims to update knowledge about the different materials used in pulp therapies (both in primary and permanent teeth) that seek for an increasingly conservative, biological and sustainable reparative response. It is a journey through the use of agents such as mineral trioxide aggregate (MTA), lasers, biodentine and platelet rich plasma concentrates, their properties, applications and efficacy, clinically and radiographically evaluated in multiple research papers, case reports, comparative studies (in vitro and in vivo) and experimental studies in animals that document their results


Assuntos
Humanos , Animais , Materiais Biocompatíveis/uso terapêutico , Polpa Dentária/efeitos dos fármacos , Plasma Rico em Plaquetas , Dente Decíduo , Dentição Permanente , Terapia a Laser
5.
Rev. bras. cir. plást ; 34(3): 349-354, jul.-sep. 2019. ilus
Artigo em Inglês, Português | LILACS | ID: biblio-1047152

RESUMO

Introdução: A produção e a distribuição de pele humana pelos Bancos de Pele do Brasil é insuficiente para atender a demanda do país, suprindo menos de 1% da necessidade para tratar as vítimas de queimaduras. O objetivo do trabalho foi apresentar a elaboração e o desenvolvimento da implantação do primeiro Banco de Pele Animal do Brasil para tratamento da queimadura. Métodos: Trata-se de um estudo metodológico. O Banco de Pele Animal Aquático foi elaborado, desenvolvido e instalado a partir do desenvolvimento do processamento de pele da tilápia, da revisão sistemática de estudos referentes a banco de pele animal, de visitas à pscicultura em Jaguaribara-CE, da visita técnica aos bancos de pele humana no Brasil, de consulta técnica e de treinamento no Banco de Pele de Recife, da observação de todas as fases de processamento da pele de tilápia e da identificação da estrutura física da área onde ocorrem todos os processos. Resultados: Além da produção e da distribuição da pele da tilápia para os estudos em vítima de queimaduras, o banco está processando mais de 5000 peles de tilápia e encontra-se em fase de distribuição desta pele para estudos multicêntricos em outros estados e outras especialidades, que desenvolvem pesquisas nas áreas de ginecologia, ortopedia, endoscopia, estomaterapia, cirurgia vascular, odontologia e veterinária. Conclusão: este trabalho possibilitou a elaboração, o desenvolvimento e a implementação do primeiro Banco de Pele Animal do país e o primeiro Banco de Pele Aquática do mundo.


Introduction: The production and distribution of human skin by Brazilian skin banks is insufficient to meet the country's demand, supplying less than 1% of the need for treating burn victims. The objective of this work was to present the elaboration and development of the first animal skin bank of Brazil for the treatment of burns. Methods: This methodological study elaborated, developed, and installed The Aquatic Animal Skin Bank in terms of the development of tilapia skin processing after a systematic review of studies referring to animal skin banks based on visits to the pisciculture center in Jaguaribara-CE, technical visits to human skin banks in Brazil, technical consultation and training in the Recife Skin Bank, the observation of all phases of tilapia skin processing, and the identification of the physical structure of the area where the processes occur. Results: In addition to the production and distribution of tilapia skin for studies on burn victims, the bank is processing more than 5000 tilapia skin samples and is in the distribution phase of this skin for multicentric studies in other states and specialties including gynecology, orthopedics, endoscopy, stomatherapy, vascular surgery, dentistry, and veterinary medicine. Conclusion: This work enabled the elaboration, development, and implementation of Brazil's first animal skin bank and the world's first aquatic skin bank.


Assuntos
Animais , História do Século XXI , Materiais Biocompatíveis , Materiais Biocompatíveis/uso terapêutico , Curativos Biológicos , Queimaduras , Tilápia , Pele Artificial , Ciclídeos , Pesqueiros , Materiais Biocompatíveis/análise , Curativos Biológicos/normas , Curativos Biológicos/provisão & distribução , Queimaduras/reabilitação , Tilápia/cirurgia , Pele Artificial/normas , Ciclídeos/cirurgia , Pesqueiros/normas
6.
Tissue Cell ; 59: 70-81, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31383291

RESUMO

Mesenchymal stem cells (MSCs) owing their multipotency are known as progenitors for the regeneration of adult tissues including that of neuronal tissue. The repair and/or regeneration of traumatic nerves is still a challenging task for neurosurgeons. It is also a well-established fact that the microenvironment plays a primary role in determining the fate of stem cells to a specific lineage. In recent years, with the advent of nanotechnology and its positive influence on designing and fabrication of various 3D biomaterials have progressed to a greater extent. The production of 3D biomaterials such as nanofibers, conduits and hydrogels are providing a suitable environment for mimicking physiological niche of stem cells. These 3D biomaterials in combination with MSCs have been successfully analyzed for their potential in the regeneration of degenerative neurological disorders. This review primarily highlights the combinatorial effect of multipotent MSCs seeded on various 3D polymeric scaffolds in repair and regeneration of nervous tissue. The elaboration of MSCs from distinct sources reported so far in literature are summarized to understand their role in regeneration processes. Furthermore, we accentuate the application of 3D biomaterials especially the nanofibers, polymeric conduits, hydrogels infiltrated with MSCs harvested from distinct sources in the field of peripheral nerve regeneration studies.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Hidrogéis/uso terapêutico , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/metabolismo , Regeneração Nervosa , Nervos Periféricos/fisiologia , Animais , Humanos
7.
Mar Drugs ; 17(8)2019 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-31405173

RESUMO

This review focuses on the expanding role of marine collagen (MC)-based scaffolds for biomedical applications. A scaffold-a three-dimensional (3D) structure fabricated from biomaterials-is a key supporting element for cell attachment, growth, and maintenance in 3D cell culture and tissue engineering. The mechanical and biological properties of the scaffolds influence cell morphology, behavior, and function. MC, collagen derived from marine organisms, offers advantages over mammalian collagen due to its biocompatibility, biodegradability, easy extractability, water solubility, safety, low immunogenicity, and low production costs. In recent years, the use of MC as an increasingly valuable scaffold biomaterial has drawn considerable attention from biomedical researchers. The characteristics, isolation, physical, and biochemical properties of MC are discussed as an understanding of MC in optimizing the subsequent modification and the chemistries behind important tissue engineering applications. The latest technologies behind scaffold processing are assessed and the biomedical applications of MC and MC-based scaffolds, including tissue engineering and regeneration, wound dressing, drug delivery, and therapeutic approach for diseases, especially those associated with metabolic disturbances such as obesity and diabetes, are discussed. Despite all the challenges, MC holds great promise as a biomaterial for developing medical products and therapeutics.


Assuntos
Organismos Aquáticos/química , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/uso terapêutico , Colágeno/farmacologia , Colágeno/uso terapêutico , Animais , Humanos , Engenharia Tecidual/métodos , Tecidos Suporte
8.
Eur Arch Otorhinolaryngol ; 276(11): 3067-3072, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31451899

RESUMO

PURPOSE: To investigate skin-related postoperative outcomes following a tissue preservation technique in percutaneous hydroxyapatite-coated bone-anchored hearing aid (BAHA) abutment implantation. METHODS: A retrospective medical records review of adult patients, who underwent single-stage BAHA implantation between July 2013 and November 2017 at a tertiary centre was conducted. Surgical procedures were performed by a single surgeon using a linear incision soft tissue preservation technique. Patients were reviewed at 1 week, 4 weeks, 3 months, 6 months, and annually postoperatively and soft tissue reactions were graded using Holger's score RESULTS: There were 102 patients included with a slight female preponderance (female:male 56:46). There were 586 follow-up episodes during the study period. From the recorded follow-up episodes, Holger's scores were documented as follows: Holger score 0 (89%); 1 (7%); 2 (2%); 3 (1.9%). Three patients (3%) required peri-abutment soft tissue excision (Holger 3) and insertion of longer abutments. One patient (1%) reported atraumatic implant loss. The BAHA was re-implanted in two patients (2%) due to traumatic dislodgement. There was a statistically significant association (p = 0.009) when the mean time to minor skin complications was compared with mean time to a significant skin reaction. CONCLUSION: Tissue preservation technique is the procedure of choice for BAHA abutment implant surgery. It confers excellent soft tissue outcomes and an excellent implant survival rate.


Assuntos
Durapatita/uso terapêutico , Auxiliares de Audição , Complicações Pós-Operatórias/prevenção & controle , Implantação de Prótese , Dermatopatias , Pele , Materiais Biocompatíveis/uso terapêutico , Prótese Ancorada no Osso , Materiais Revestidos Biocompatíveis/uso terapêutico , Feminino , Perda Auditiva/cirurgia , Humanos , Masculino , Pessoa de Meia-Idade , Tratamentos com Preservação do Órgão/efeitos adversos , Tratamentos com Preservação do Órgão/métodos , Osseointegração , Implantação de Prótese/efeitos adversos , Implantação de Prótese/métodos , Reoperação/estatística & dados numéricos , Estudos Retrospectivos , Dermatopatias/etiologia , Dermatopatias/prevenção & controle
9.
Arq. bras. med. vet. zootec. (Online) ; 71(4): 1121-1130, jul.-ago. 2019. graf, ilus
Artigo em Português | LILACS, VETINDEX | ID: biblio-1038624

RESUMO

O objetivo do estudo foi avaliar o efeito da matriz porosa do biovidro 60S (BV60S) associada a células osteoprogenitoras (CO) alógenas no tratamento de defeitos ósseos críticos de cães. Foram utilizados 20 cães, machos, sem raça definida, com dois anos de idade e massa corporal média de 25kg. Com os cães sob anestesia geral, foram criados defeitos ósseos críticos no terço médio dos ossos rádios. Procedeu-se à fixação óssea com uma placa em ponte, e os defeitos foram tratados de acordo com cada grupo experimental. Constituíram-se três grupos experimentais, em que os defeitos ósseos foram preenchidos com: BV60S associado a CO alógenas (grupo BV60S+CO), osso autógeno (grupo C+), ou não preenchidos (grupo C-). A regeneração óssea foi avaliada por meio de exames radiográficos, densitométricos e histomorfométricos ao longo de 90 dias. Os grupos C- e BV60S+CO mostraram preenchimento ósseo parcial do defeito de, no máximo, 56,68% e 35,23%, respectivamente, sem a formação de ponte óssea entre as extremidades, e o controle positivo (C+) mostrou regeneração óssea completa. Conclui-se que a matriz porosa do BV60S associada às células osteoprogenitoras não é eficiente no tratamento de defeitos ósseos críticos em rádios de cães.(AU)


The objective of this study was to evaluate the effect of the porous matrix of bioglass 60S (BV60S) associated with allogenic osteoprogenitor cells (CO) in the treatment of critical bone defects of dogs. 20 male mongrel dogs at two years old and mean weight of 25kg were used. Dogs were anesthetized and critical bone defects were created in the middle third of the radios bones. With dogs under general anesthesia, critical bone defects were created in the middle third of bone radios. Bone fixation was done with a bridge plate and defects treated according to each experimental group. Three experimental groups were formed according to the treatment. The defects filled with BV60S associated with allogenic CO (Group-BV60S+CO), autogenous bone (Group-C+) or unfilled (Group-C-). Bone regeneration was evaluated by radiography, bone densitometry and histomorphometry over 90 days. The BV60S+CO and C- groups showed partial bone filling of the defect of at most 56.68% and 35.23%, respectively. No bone bridge formation was observed between the extremities in the BV60S+CO and C- groups. Positive control showed complete bone regeneration at 90 days. It was concluded that the porous matrix of BV60S associated with osteoprogenitor cells was not effective in the treatment of critical bone defects in the radius of dogs.(AU)


Assuntos
Animais , Cães , Rádio (Anatomia)/lesões , Materiais Biocompatíveis/uso terapêutico , Regeneração Óssea , Terapia Baseada em Transplante de Células e Tecidos/métodos , Terapia Baseada em Transplante de Células e Tecidos/veterinária
10.
Int J Mol Sci ; 20(15)2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31344903

RESUMO

Chronic back pain is a common disability, which is often accredited to intervertebral disc degeneration. Gold standard interventions such as spinal fusion, which are mainly designed to mechanically seal the defect, frequently fail to restore the native biomechanics. Moreover, artificial implants have limited success as a repair strategy, as they do not alter the underlying disease and fail to promote tissue integration and subsequent native biomechanics. The reported high rates of spinal fusion and artificial disc implant failure have pushed intervertebral disc degeneration research in recent years towards repair strategies. Intervertebral disc repair utilizing principles of tissue engineering should theoretically be successful, overcoming the inadequacies of artificial implants. For instance, advances in the development of scaffolds aided with cells and growth factors have opened up new possibilities for repair strategies. However, none has reached the stage of clinical trials in humans. In this review, we describe the hitches encountered in the musculoskeletal field and summarize recent advances in designing tissue-engineered constructs for promoting nucleus pulposus repair. Additionally, the review focuses on the effect of biomaterial aided with cells and growth factors on achieving effective functional reparative potency, highlighting the ways to enhance the efficacy of these treatments.


Assuntos
Dor nas Costas/genética , Núcleo Celular/genética , Degeneração do Disco Intervertebral/terapia , Núcleo Pulposo/metabolismo , Dor nas Costas/terapia , Materiais Biocompatíveis/uso terapêutico , Humanos , Disco Intervertebral/fisiopatologia , Degeneração do Disco Intervertebral/genética , Núcleo Pulposo/patologia , Engenharia Tecidual
11.
Mater Sci Eng C Mater Biol Appl ; 103: 109858, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31349473

RESUMO

Mandibles are the largest and strongest bone in the human face and are often severely compromised by mandibular defects, compromising the quality of life of patients. Mandibular defects may result from trauma, inflammatory disease and benign or malignant tumours. The reconstruction of mandibular defect has been a research hotspot in oral and maxillofacial surgery. Although the principles and techniques of mandibular reconstruction have made great progress in recent years, the development of biomedical materials is still facing technical bottleneck, and new materials directly affect technological breakthroughs in this field. This paper reviews the current status of research and application of various biomaterials in mandibular defects and systematically elaborates different allogeneic biomaterial-based approaches. It is expected that various biomaterials, in combination with new technologies such as digital navigation and 3D printing, could be tuned to build new types of scaffold with more precise structure and components, addressing needs of surgery and post-reconstruction. With the illustration and systematization of different solutions, aims to inspire the development of reconstruction biomaterials.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Mandíbula/cirurgia , Traumatismos Mandibulares/cirurgia , Reconstrução Mandibular , Impressão Tridimensional , Humanos
12.
Carbohydr Polym ; 222: 114985, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31320093

RESUMO

In recent years, bacterial cellulose (BC)-based dressings or patches for skin or soft tissue repair have become investigative emphasis. However, most of the BC-based products used for biomedical applications present limitations due to their low flexibility, poor gas permeability and no inherent antibacterial activity. Herein, we proposed and designed a novel composite composed of natural bacterial cellulose (BC), polyethylene glycol (PEG) and polyhexamethylene biguanidine (PHMB) through new synthetic approaches. The composite membrane exhibited favorable physicochemical performance, especially transparency, water retention ability, flexibility as well as the characteristic of anti-adhesion. In vitro biochemical experiment results indicated that the composite had excellent biocompatibility and exhibited strong and sustained antibacterial effect. In vivo test further demonstrated that the composite could efficiently promote skin wound healing and regeneration in a rat model. This composite membrane possesses multiple mechanisms of promoting cutaneous wound healing and will provide new ideas for future development of wound dressings.


Assuntos
Antibacterianos/química , Bandagens , Biguanidas/química , Materiais Biocompatíveis/uso terapêutico , Celulose/química , Polietilenoglicóis/química , Cicatrização , Animais , Antibacterianos/farmacologia , Biguanidas/farmacologia , Celulose/farmacologia , Escherichia coli , Células Endoteliais da Veia Umbilical Humana , Humanos , Camundongos , Polietilenoglicóis/farmacologia , Pele/efeitos dos fármacos , Staphylococcus aureus
13.
Georgian Med News ; (290): 131-135, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31322530

RESUMO

Reconstruction of mandibular defects is a challenging issue in maxillofacial surgery. Success of the treatment is defined by the process of reparative regeneration of bone tissue, which is often accompanied with wound infection, failure of microcirculation and tissue hypoxia. Aim of our project was to demonstrate the advantage of osteoplastic material and bone marrow derived mesenchymal stem cells combination in regeneration of lower jaw bone defect. For this reason, mandibular bone defect was created in laboratory rats. Experimental treatment of animals was performed in three different ways: group 1. - Bone defect was regenerated without any treatment, group 2. - Bone defect was filled with osteoplastic material (BIO-OSS), group 3. - Bone defect was filled with BIO-OSS, which was previously infiltrated by bone marrow mesenchymal stem cells. Animals were under observation for 6 months. During this period bone regeneration was comparatively analyzed in these tree experimental groups. Observational, histochemical and x-ray research methods were used in this study. Our results suggest that use of osteoplastic material and mesenchymal stem cells combination can increase formation of red bone marrow, which is normally slow under natural condition. Therefore, we can conclude that application of osteoplastic material with bone marrow derived mesenchymal stem cells can decrease time of bone defect osteointegration and define the quality of regenerated bone tissue.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Regeneração Óssea , Substitutos Ósseos , Mandíbula/cirurgia , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais , Animais , Osso e Ossos/patologia , Osso e Ossos/cirurgia , Mandíbula/patologia , Ratos , Engenharia Tecidual
14.
ACS Appl Mater Interfaces ; 11(29): 25637-25653, 2019 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-31283160

RESUMO

Toward imitating the natural smartness and responsivity of biological systems, surface interfacial properties are considered to be responsive and tunable if they show a reactive behavior to an environmental stimulus. This is still quite different from many contemporary biomaterials that lack responsiveness to interact with blood and different body tissues in a physiological manner. Meanwhile it is possible to even go one step further from responsiveness to dual-mode switchability and explore "switchable" or "reversible" responses of synthetic materials. We understand "switchable biomaterials" as materials undergoing a stepwise, structural transformation coupled with considerable changes of interfacial and other surface properties as a response to a stimulus. Therewith, a survey on stimuli-induced dynamic changes of charge, wettability, stiffness, topography, porosity, and thickness/swelling is presented here, as potentially powerful new technologies especially for future biomaterial development. Since living cells constantly sense their environment through a variety of surface receptors and other mechanisms, these obedient interfacial properties were particularly discussed regarding their advantageous multifunctionality for protein adsorption and cell adhesion signaling, which may alter in time and with environmental conditions.


Assuntos
Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Sistemas de Liberação de Medicamentos , Molhabilidade
15.
Biomed Res Int ; 2019: 2519205, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31360706

RESUMO

Dental implants are often made of titanium alloys. Implant therapy currently promises a good long-term result without impacting health; however, its success depends on many factors. In this article, the authors focus on the most common risk factors associated with metallic surgical implants. Titanium-induced hypersensitivity can lead to symptoms of implant rejection. Corrosion and biofilm formation are additional situations in which these symptoms may occur. For medical purposes, it is important to define and discuss the characteristics of metals used in implantable devices and to ensure their biocompatibility. To avoid hypersensitivity reactions to metallic dental implants, precautionary principles for primary prevention should be established.


Assuntos
Materiais Biocompatíveis/efeitos adversos , Implantes Dentários/efeitos adversos , Metais/efeitos adversos , Materiais Biocompatíveis/uso terapêutico , Corrosão , Ligas Dentárias/efeitos adversos , Ligas Dentárias/uso terapêutico , Humanos , Metais/uso terapêutico , Fatores de Risco , Titânio/efeitos adversos , Titânio/uso terapêutico
16.
Clin Orthop Surg ; 11(2): 233-236, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31156777

RESUMO

Background: We investigated the clinical outcome in patients whose cavitary bone defects were treated with beta-tricalcium phosphate (ß-TCP) after surgical removal of benign tumors. Methods: Between March 2015 and December 2015, 20 patients who underwent operation for bone tumors were enrolled into this study and prospectively followed up for a median period of 28.1 months. Results: When the radiographic sign of complete resorption was defined as greater than 50% resorption of the allograft material accompanied by bone remodeling until 12 months, 55% of patients had complete resorption. Positive correlation between the filling volume and time needed for complete resorption was not found (p = 0.184). Conclusions: Purified ß-TCP could be a suitable choice as a bone graft substitute after the removal of benign bone tumors.


Assuntos
Materiais Biocompatíveis/uso terapêutico , Neoplasias Ósseas/cirurgia , Substitutos Ósseos/uso terapêutico , Fosfatos de Cálcio/uso terapêutico , Adulto , Neoplasias Ósseas/diagnóstico por imagem , Neoplasias Ósseas/patologia , Transplante Ósseo , Feminino , Humanos , Masculino , Estudos Prospectivos
17.
Chem Soc Rev ; 48(13): 3513-3536, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31157810

RESUMO

Bioconjugates are multifunctional constructs in which biomolecules like peptides, proteins, vitamins and nucleic acids are endowed with the properties of specific payloads. These constructs recently emerged as a new generation of high-precision therapeutics, with several representatives reaching the market. This success stimulated an intense search for new biocompatible synthetic methodologies to connect both components and to control the bioconjugate's function. Despite the remarkable advances made in this field, most of the technologies developed for the construction of bioconjugates were engineered to yield stable constructs that can endure complex physiological conditions. Because of this, the use of reversible covalent bonds in the synthesis of bioconjugates has been rather overlooked, notwithstanding the potential of this strategy to generate stimuli responsive constructs that may operate in areas like the selective delivery of drugs, live-cell imaging and new theranostic approaches. Boronic acids are a well-known class of reagents that have been widely used in modern synthesis for the formation of C-C and C-heteroatom bonds. Apart from this, boronic acids exhibit an exquisite reversible coordination profile that can be explored as a molecular construction tool featuring specific mechanisms to control the structure and biological properties of bioconjugates. In this review, the use of boronic acids in the construction of therapeutically useful bioconjugates will be discussed, focusing on the molecular mechanisms that allow the use of these reagents as bioconjugation warheads, as central pieces of linker structures and as functional payloads.


Assuntos
Materiais Biocompatíveis/química , Ácidos Borônicos/química , Animais , Materiais Biocompatíveis/uso terapêutico , Ácidos Borônicos/uso terapêutico , Sistemas de Liberação de Medicamentos , Desenho de Drogas , Humanos , Nanomedicina Teranóstica
18.
Nat Med ; 25(6): 898-908, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31160817

RESUMO

Individuals with spinal cord injury (SCI) can face decades with permanent disabilities. Advances in clinical management have decreased morbidity and improved outcomes, but no randomized clinical trial has demonstrated the efficacy of a repair strategy for improving recovery from SCI. Here, we summarize recent advances in biological and engineering strategies to augment neuroplasticity and/or functional recovery in animal models of SCI that are pushing toward clinical translation.


Assuntos
Traumatismos da Medula Espinal/terapia , Animais , Materiais Biocompatíveis/uso terapêutico , Bioengenharia , Modelos Animais de Doenças , Humanos , Modelos Neurológicos , Plasticidade Neuronal , Recuperação de Função Fisiológica , Traumatismos da Medula Espinal/fisiopatologia , Traumatismos da Medula Espinal/reabilitação , Regeneração da Medula Espinal , Pesquisa Médica Translacional
19.
Folia Med (Plovdiv) ; 61(1): 34-40, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31237853

RESUMO

This survey presents a thorough overview of the main types of biomaterials used for the manufacturing of implants. The use of different materials for the creation and refinement of medical devices aims at optimizing their properties and raising the level of safety for the patients. The purpose of the study is to classify the most common bulk materials used in medicine according to their nature, interaction with the host tissues and their function in the organisms. Some important advantages and disadvantages of the different classes of implant materials are considered. In the last few years there is a strong tendency toward the surface modification of biomedical devices. Various trends in processing of the materials are focused on increasing their corrosion resistance, wear resistance, biocompatibility and microbiological properties.


Assuntos
Materiais Biocompatíveis , Ligas , Materiais Biocompatíveis/uso terapêutico , Cerâmica , Humanos , Polímeros , Próteses e Implantes , Aço Inoxidável
20.
Carbohydr Polym ; 220: 219-227, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31196543

RESUMO

Burns and chronic wounds, often related to chronic diseases (as diabetes and cancer), are challenging lesions, difficult to heal. The prompt and full reconstitution of a functional skin is at the basis of the development of biopolymer-based scaffolds, representing a 3D substrate mimicking the dermal extracellular matrix. Aim of the work was to develop scaffolds intended for skin regeneration, according to: fabrication by electrospinning from aqueous polysaccharide solutions; prompt and easy treatment to obtain scaffolds insoluble in aqueous fluids; best performance in supporting wound healing. Three formulations were tested, based on chitosan (CH) and pullulan (P), associated with glycosaminoglycans (chondroitin sulfate - CS or hyaluronic acid - HA). A multidisciplinary approach has been used: chemico-physical characterization and preclinical evaluation allowed to obtain integrated information. This supports that CS gives distinctive properties and optimal features to the scaffold structure for promoting cell proliferation leading tissue reparation towards a complete skin restore.


Assuntos
Materiais Biocompatíveis/química , Quitosana/química , Sulfatos de Condroitina/química , Glucanos/química , Ácido Hialurônico/química , Engenharia Tecidual , Tecidos Suporte , Cicatrização , Materiais Biocompatíveis/uso terapêutico , Queimaduras/terapia , Humanos , Pele Artificial
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