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1.
Gen Dent ; 70(1): 22-25, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34978985

RESUMO

The aim of this study was to compare the effectiveness of a reciprocating file (Reciproc) and a rotary retreatment file (Mtwo retreatment [Mtwo-R]) for the removal of filling material during root canal retreatment. A total of 30 mandibular molars with a mesial root curvature between 20° and 40° were selected and prepared using a Reciproc R25 file. The canals were filled using the lateral condensation technique and a root canal sealer. The specimens were randomly allocated into 2 groups according to the retreatment technique used (n = 15): a Reciproc R25 file or Mtwo-R 15/.05 and 25/.05 files. After retreatment, the specimens were longitudinally sectioned and photographed with an operating microscope under ×10 magnification. The percentage of total remaining filling material and the percentages in the cervical, middle, and apical thirds of the specimens were calculated. The Student t test was used for statistical comparisons of the 2 groups, and the analysis of variance followed by Tukey test was used to verify differences among the root thirds within each group. No statistically significant difference between the 2 groups was detected in the percentage of total remaining filling material (P = 0.87) or the percentage in any of the thirds of the root canals (P > 0.05) after reinstrumentation. In both groups, there was a significantly greater amount of remaining material in the apical third (P < 0.05). The results suggested that there is no difference between the 2 systems in their effectiveness at removing filling material.


Assuntos
Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Cavidade Pulpar , Desenho de Equipamento , Guta-Percha , Humanos , Retratamento , Preparo de Canal Radicular
2.
Gen Dent ; 70(1): 40-44, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-34978989

RESUMO

This study sought to compare a bioceramic sealer (TotalFill) and a calcium hydroxide (Ca[OH]2) sealer (Sealapex) in terms of their pH, calcium ion (Ca²âº) release, and antibacterial effect against Enterococcus faecalis bacteria. For the pH and Ca²âº release tests, 20 polyethylene tubes (10 mm in height and 1 mm in internal diameter) were filled with the appropriate sealer (n = 10 per sealer), immersed in glass flasks each containing 10 mL of deionized water, and stored in an incubator at 37°C. The water was changed after 1, 7, 28, and 90 days. At each water change, the eluates were measured with an advanced electrochemistry meter to determine the pH and with a flame atomic absorption spectrometer to determine the Ca²âº release. The antibacterial effect was measured using the turbidimetry-based direct contact test in which the wells of a microtiter plate were coated with a thin, even layer of freshly prepared sealer (10 wells per sealer), which was allowed to set before application of a suspension of E faecalis. Control wells were obtained by placing an identical bacterial suspension in 10 uncoated wells. The optical density of the sealer and control groups was measured immediately and 1, 3, and 7 days after sealer preparation. Data were analyzed for normality with the Shapiro-Wilk and Kolmogorov-Smirnov tests. Two-way analysis of variance (ANOVA), Student t test, and 1-way ANOVA with Tukey post hoc tests were all utilized with a significance level of P < 0.05. TotalFill maintained significantly higher pH and Ca²âº release levels than Sealapex at all evaluation times (P < 0.05). Both sealers demonstrated significantly greater antibacterial effect (lower optical density) than the control group; however, TotalFill resulted in significantly lower optical density values than Sealapex (P < 0.05). TotalFill bioceramic sealer demonstrated superior Ca(OH)2-related properties compared to Sealapex Ca(OH)2 sealer.


Assuntos
Cálcio , Materiais Restauradores do Canal Radicular , Antibacterianos/farmacologia , Resinas Epóxi , Humanos , Concentração de Íons de Hidrogênio , Teste de Materiais , Materiais Restauradores do Canal Radicular/farmacologia , Silicatos
3.
Ned Tijdschr Tandheelkd ; 129(1): 33-40, 2022 Jan.
Artigo em Holandês | MEDLINE | ID: mdl-35015390

RESUMO

When endodontic treatment of periapical infection does not suffice, apicoectomy might be the next treatment of choice. Years of treatment development have optimised it as far as possible, but the optimal apical barrier material has still not been indicated. Does MTA as an apical barrier material in apicoectomies effectuate a higher success rate than IRM? A retrospective cohort study analysed all patients who underwent an apicoectomy between 2015 and 2020. Patients for whom the apical barrier material had not been registered, or who were treated by a resident, were excluded. Patients were retrospectively allocated to either the MTA or IRM group. A total of 1,347 treatments were analysed, with an overall success rate of 71%. The MTA group had a success rate of 71%, and the IRM group had a success rate of 70% (p = 0.794). The study revealed that the success rate of apicoectomies was 71%, regardless of the type of material used as the apical barrier. Hence, the use of either material is justified.


Assuntos
Apicectomia , Materiais Restauradores do Canal Radicular , Compostos de Alumínio , Compostos de Cálcio , Combinação de Medicamentos , Humanos , Óxidos , Pemetrexede , Estudos Retrospectivos , Silicatos
4.
J Mech Behav Biomed Mater ; 125: 104971, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34798534

RESUMO

OBJECTIVE: This study evaluated the influence of adding bioactive glasses particles [Niobophosphate (NbG) or bioglass (45S5)] into endodontic cements in relation to physical, chemical and biological properties. METHODS: The following commercial cements were used as comparison: AH Plus (Dentsply), Endofill (Dentsply), MTA Fillapex (Angelus) and EndoSequence (BC Sealer, Brasseler). Setting time, radiopacity, flow rate, weight loss/variation, alkaline capacity (pH) at different time-intervals (24h/48h/7d/14d/28d), bioactivity (assessed under SEM/EDS, FTIR/ATR and XDR) and cell viability were measured. Data were analyzed by One-way ANOVA/Holm-Sidak post-test (α = 5%) (normal distribution) and Kruskal-Wallis/Students-Newman-Keuls post-test (α = 5%) (non-normal distribution). RESULTS: Bioactive endodontic experimental cements (containing NbG or 45S5) had high alkalinization capacity. The experimental cements presented high weight loss/variation (p < 0.001). 45S5 experimental cement did not present radiopacity (p < 0.001). AH Plus had the lowest cell cytotoxicity when compared to the other tested cements (p < 0.001). Regarding bioactivity, SEM/EDS analyses showed precipitates with high concentrations of Ca/P for 45S5 and NbG, as well as for MTA Fillapex and BC Sealer. AH plus and Endofill did not present bioactive precipitates. FTIR/ATR and XDR analyses found hydroxyapatite precursors for NbG, 45S5, MTA Fillapex and BC Sealer. SIGNIFICANCE: The incorporation of bioactive particles (NbG or 45S5) into endodontic cements had potential to neutralize acidic environments and induced formation of hydroxyapatite precursors. Clinically, these would produce a cement that is bactericidal and have the potential to improve tissue healing. The improved radiopacity and flowability would facilitate the visualization of the material in the radiograph and the filling of anatomical complexities during root canal obturation. As drawbacks, the excessive weight loss and post-setting cytotoxicity could result in clinical degradation of the cement and adjacent tissue irritation for the patient.


Assuntos
Materiais Restauradores do Canal Radicular , Cimentos Ósseos , Durapatita , Cimentos de Ionômeros de Vidro , Humanos
5.
Pediatr Dent ; 43(6): 435-442, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34937613

RESUMO

Purpose: The purpose of this study was to compare the effectiveness of lesion sterilization and tissue repair (LSTR) antibiotic paste comprised of chloramphenicol, tetracycline, and zinc oxide and eugenol (CTZ) versus zinc oxide eugenol (ZOE) pulpectomy in the treatment of primary molars with pulp necrosis. Methods: A total of 70 three- to eight-year-old subjects with 88 primary mandibular molars with pulp necrosis were included. The teeth were randomized to the CTZ group or ZOE group. The time taken to perform both techniques was recorded. The parents of the children and the dentist who performed clinical evaluations were blind to the group assignment, although the radiographic evaluator could see the difference in treatments. Clinical and radiographic assessments were performed at three, six, nine, and 12 months. Results: At the 12-month evaluation, the clinical success was 86.4 percent for CTZ and 90.9 percent for ZOE (P=0.50), the radiographic success was 75.0 percent for CTZ and 72.7 percent for ZOE (P=0.81), and the overall success was 70.5 percent for CTZ and 72.7 percent for ZOE (P=0.81). The mean time taken to perform was 61.4 (±20.5 standard deviation) minutes for CTZ and 145.1 (±53.2) minutes for ZOE (P<0.001). Conclusions: At 12 months, both techniques presented no significant difference in success rates for nonvital pulp therapy in primary molars with necrosis. The lesion sterilization and tissue repair procedure time using chloramphenicol, tetracycline, zinc oxide, and eugenol was significantly shorter than for a zinc oxide eugenol pulpectomy.


Assuntos
Materiais Restauradores do Canal Radicular , Óxido de Zinco , Antibacterianos/uso terapêutico , Criança , Pré-Escolar , Necrose da Polpa Dentária/terapia , Eugenol/uso terapêutico , Humanos , Dente Molar/diagnóstico por imagem , Dente Molar/cirurgia , Pulpectomia , Esterilização , Dente Decíduo , Óxido de Zinco/uso terapêutico , Cimento de Óxido de Zinco e Eugenol/uso terapêutico
6.
Braz. j. oral sci ; 20: e210432, jan.-dez. 2021. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1254269

RESUMO

Aim: The aim of this study was to evaluate, by micro-computed tomography (micro-CT) analysis, the remaining filling material during endodontic retreatment performed with Protaper retreatment without solvent. Methods: Forty mandibular molars were divided into two groups (n = 20) according to the sealer used in the obturation: the bioceramic TotalFill BC (TF) or the resin-based AH Plus (AHP). The specimens were scanned before instrumentation, after obturation and after filling removal. Only the mesial roots were analysed. The filling volumes and the remaining filling material were calculated in the entire root canal and in the cervical, middle and apical thirds. Results: The volume of obturation and the volume of remaining filling material in the entire root canal and in the cervical, middle and apical thirds of the canal between the groups were not statistically different (independent t-test, p > 0.05). In the AHP group, there was a higher percentage of remaining filling material in the middle third than in the cervical third (p < 0.05). Conclusion: The filling material could not be entirely removed from any specimen


Assuntos
Materiais Restauradores do Canal Radicular , Retratamento , Microtomografia por Raio-X , Dente Molar
7.
Braz. j. oral sci ; 20: e212098, jan.-dez. 2021. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1281101

RESUMO

Aim: This study aimed to evaluate the pH, Ca2+ release, solubility, and antimicrobial activity of calcium hydroxide (Ca(OH)2) pastes in association with different substances. Methods: Sixty acrylic teeth (n=10) were filled with pastes that associated Ca(OH)2 with the following substances: benzalkonium chloride 5% (G1) and 50% (G2) both in propylene glycol, arnica glycolic extract (G3), green tea glycolic extract (G4), Calen/PMCC™ (G5), and Calen™ (G6). In the group G1 to G4 were used 1g of Ca(OH)2 powder with 0,8g of vehicle. pH and Ca2+ release was measured after 7, 15, and 30 days. For solubility, micro-CT was used immediately and at the periods of 7, 15, and 30 days. For the antimicrobial analysis, a biofilm of E. faecalis was induced in vitro on bovine dentin discs. Live/dead viability dye and confocal scanning microscopy were used. Results: The highest pH values occurred on the first 7 days, and the G6, G1, G3, and G5 presented the highest pH values at this period (P <0.05). Ca2+ release was higher in all groups at 7 days, with the highest values observed in G1, G5, and G6. The volume of all pastes showed no significant difference in the intragroup analysis at 7 and 15 days (P <0.05). G1 and G2 showed the highest antimicrobial action (P <0.05). For the biovolume, there was difference between the G6 and the other groups (P >0.05) with G1 presenting the lowest values. Conclusion: Benzalkonium chloride 5% increases the antimicrobial action of the Ca(OH2), without impairs physicochemical properties


Assuntos
Materiais Restauradores do Canal Radicular , Hidróxido de Cálcio , Biofilmes , Fenômenos Químicos , Anti-Infecciosos
8.
Braz. j. oral sci ; 20: e214073, jan.-dez. 2021. ilus
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1282556

RESUMO

Aim: this study aimed to compare the sealing ability of two types of commercially available calcium silicate bioceramic based root canal sealers and a resin based root canal sealer. Methods: Twenty one single-rooted teeth were used, samples (n= 21) were randomly divided into three groups according to the sealer used (group A; ADSEAL, group B; Wellroot, group C; Ceraseal). Roots were then cleaved longitudinally in the labiolingual direction; all samples were then sectioned at three, six, and nine mm from the root tip. The penetration of sealers into the dentinal tubules was examined at 1000x with a scanning electron microscope. Data were tested for normality using Shapiro Wilk test. ANOVA test was used for analyzing normally distributed data followed by Bonferroni post hoc test for pair-wise comparison. Significance level p≤0.001. Results: groups B and C showed better sealing ability than group A in all the three sections. The coronal section showed higher sealing ability than the middle section followed by the apical section in the three tested groups. Conclusion: it can be concluded that both calcium silicate-based sealers had better sealing ability and higher bond strength than the resin epoxy- based sealer


Assuntos
Materiais Restauradores do Canal Radicular , Silicatos , Compostos de Cálcio , Resinas Epóxi , Tomografia com Microscopia Eletrônica
9.
Rev. Odontol. Araçatuba (Impr.) ; 42(3): 4-8, set.-dez. 2021. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1283897

RESUMO

Introdução: Os cones de guta percha são utilizados para obturação de canais radiculares, entretanto a desinfecção desses cones deve ser realizada previamente à obturação, evitando uma contaminação, uma vez que com o manuseio diário na clínica, pode ocorrer a proliferação de microrganismos. Objetivo: Relatar através de uma revisão de literatura a importância da desinfecção de cones de guta percha previamente á obturação dos canais radiculares, com diferentes soluções desinfetantes utilizadas na odontologia. Metodologia: Foi realizada uma busca em artigos de revisão de literatura nas bases de dados Pubmed, Scielo, Lilacs com diferentes anos de publicação, mas todos com enfoque principal no tema de desinfecção de cones de guta percha com diferentes soluções químicas. Foram utilizados os descritores guta percha, desinfecção, soluções químicas. Conclusão: Medidas de prevenção devem ser empregadas durante o atendimento odontológico, em especial durante a obturação dos canais radiculares, sendo a desinfecção dos cones de guta percha essencial(AU)


Introduction: Gutta percha points are used to fill root canals, however its disinfection must be performed prior to filling, avoiding contamination, since with daily handling in the proliferation of microorganisms can occur. Objective: To report through a literature review the importance of disinfecting gutta percha points prior to root canal filling, with different disinfectant solutions used in dentistry. Methodology: A search for literature review articles was carried out in the Pubmed, Scielo, Lilacs databases with different years of publications, but all with a main focus on the topic of disinfection of gutta-percha points with different chemical solutions. The keywords gutta percha, disinfection, chemical solutions were used. Conclusion: Preventive measures must be used during dental care, especially during root canal filling, with the disinfection of gutta percha points being an essential(AU)


Assuntos
Irrigantes do Canal Radicular , Obturação do Canal Radicular , Guta-Percha , Materiais Restauradores do Canal Radicular , Cavidade Pulpar
10.
Rev. Odontol. Araçatuba (Impr.) ; 42(3): 32-38, set.-dez. 2021. ilus, tab
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1284132

RESUMO

A manutenção dos dentes decíduos na cavidade bucal até o período de sua esfoliação fisiológica é de extrema importância. A terapia endodôntica é um tratamento indicado quando a saúde do tecido pulpar é comprometida e diversas técnicas e materiais podem ser empregados para realizar a obturação de canais radiculares. O objetivo deste estudo in vitro foi comparar a qualidade do preenchimento dos canais radiculares utilizando diferentes técnicas de obturação e pastas obturadoras em dentes decíduos bovinos submetidos à pulpectomia. Foram selecionados 30 incisivos decíduos bovinos, e preparados através da técnica manual com posterior obturação dos canais radiculares por meio de duas técnicas e três pastas obturadoras. Os dentes foram divididos em 6 grupos (n= 5/ grupo) da seguinte forma: GI ­ Técnica manual e pasta de Hidróxido de Cálcio PA espessada com Óxido de Zinco; GII ­ Técnica manual e pasta Guedes-Pinto Modificada; GIII ­ Técnica manual e pasta Feapex®; GIV ­ Técnica com auxílio da lentulo e pasta de Hidróxido de Cálcio PA espessada com Óxido de Zinco; GV ­ Técnica com auxílio da lentulo e pasta Guedes-Pinto Modificada; GVI ­ Técnica com auxílio da lentulo e pasta Feapex®. Foram realizadas as radiografias digitais para verificar a qualidade do preenchimento dos canais radiculares. Os resultados obtidos apresentaram diferenças significativas na qualidade do preenchimento dos canais radiculares entre os grupos de dentes decíduos bovinos obturados. Considerando os resultados obtidos e a metodologia empregada, pode-se concluir que existe diferença entre as técnicas de obturação e pastas obturadoras(AU)


Maintaining primary teeth in the oral cavity until the period of their physiological exfoliation is extremely important. An endodontic therapy is a treatment indicated when the health of the pulp tissue is compromised and several techniques and materials can be used to perform root canal filling. The aim of this in vitro study was to compare the quality of root canal filling using different filling techniques and filling masses in primary bovine teeth implanted under pulpectomy. Thirty bovine primary incisors were selected and prepared using the manual technique with subsequente filling of the root canals using two techniques and three filling materials. The teeth were divided into 6 groups (n= 5 / group) as follows: GI - Technical Manual and Calcium Hydroxide Mass PA thickened with Zinc Oxide; GII - Manual technique and modified Guedes-Pinto pasta; GIII - Manual technique and Feapex® paste; GIV - Technique with the aid of lentulo and mass of Calcium Hydroxide PA thickened with Zinc Oxide; GV ­ Technique with the aid of lentulo and modified Guedes-Pinto mass; GVI - Technique with the aid of lentulo and Feapex® mass. Digital radiographs were taken to check the quality of the filling of the root canals. The resulting results dissipated in the quality of filling the root canals between the groups of primary bovine teeth filled. Know the results obtained and the methodology used, it can be concluded that there is a difference between filling techniques and filling masses(AU)


Assuntos
Pulpectomia , Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Dente Decíduo , Tratamento do Canal Radicular , Óxido de Zinco , Hidróxido de Cálcio , Cavidade Pulpar
11.
Rev. Odontol. Araçatuba (Impr.) ; 42(3): 39-44, set.-dez. 2021. ilus
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1291668

RESUMO

O objetivo do presente estudo in vitro foi avaliar, por meio de radiografia digital, a qualidade de preenchimento dos canais de dentes incisivos bovinos permanentes jovens quando realizado tampão apical com hidróxido de cálcio e MTA associados a outros materiais e posteriormente obturados. Foram utilizados dentes incisivos permanentes bovinos com rizogênese incompleta preparados de forma manual com as limas tipo K, irrigados com hipoclorito de sódio 1% e soro fisiológico. Após, foram utilizados os materiais indicados para confecção do tampão apical: hidróxido de cálcio P.A associado ao propilenoglicol e ao iodofórmio; hidróxido de cálcio P.A juntamente com MTA e propilenoglicol e somente MTA, em seguida obturados com cones de guta percha e cimento AH Plus e radiografados para avaliação. Os dados foram submetidos ao teste Qui-Quadrado de Pearson e Teste exato de Fisher com nível de significância de 5%. Os resultados obtidos não apresentaram diferença estatisticamente significativa entre os três grupos, sendo que o valor resultado foi de p=0,442, porém o grupo composto pelo cimento de hidróxido de cálcio P.A associado ao MTA e propilenoglicol apresentou uma qualidade de preenchimento boa (100 %) em relação aos outros cimentos avaliados, que se mostraram satisfatório (40%) e bom (60%) no procedimento de apicificação. Não houve diferença significativa de qualidade de preenchimento de tampão apical nos grupos, no entanto o grupo composto pelo hidróxido de cálcio P.A. associado ao MTA e propilenoglicol apresentou resultado superior(AU)


The objective of the present in vitro study was to evaluate, through digital radiography, the quality of filling in the young permanent bovine incisor teeth canals when the apical buffer was performed with calcium hydroxide and MTA associated with other materials and subsequently filled. Permanent bovine incisor teeth with incomplete rhizogenesis were prepared manually with K files type, irrigated with 1% sodium hypochlorite and saline. Afterwards, the materials indicated for making the apical buffer were used: calcium hydroxide P.A associated with propylene glycol and iodoform; calcium hydroxide P.A together with MTA and propylene glycol and only MTA, then filled with gutta-percha cones and AH Plus cement and radiographed for evaluation. The data were submitted to the Pearson's Chi-square test and Fisher's exact test with a significance level of 5%, once the result value was p=0,442. The results obtained showed no statistically significant difference between the three groups, the result being p=0,442, however the group composed of calcium hydroxideP.A. cement associated with MTA and propylene glycol showed a good filling quality (100%) in relation to the other evaluated cements, which were satisfactory (40%) and good (60%) in apexification procedure. There was no significant difference in quality of plug apical filling in the groups, however the group composed of calcium hydroxide P.A cement associated with MTA and propylene glycol showed a superior result(AU)


Assuntos
Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Tratamento do Canal Radicular , Hidróxido de Cálcio
12.
Braz Dent J ; 32(4): 1-7, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34787245

RESUMO

This study evaluated the 3D quality of root-end filling, assessing the presence (volume and percentage) of marginal gaps and internal voids formed after retro-filling with three calcium silicate-based materials: MTA Angelus (Angelus Soluçoes Odontologicas, Londrina, PR, Brazil), Biodentine (Septodont Ltd., Saint Maur-des-Faussés, France) and Neo MTA Plus (Avalon Biomed Inc., Bradenton, Florida, US). Thirty human, extracted, single rooted teeth were used. Orthograde root canal treatment, root resection (3mm shorter than the apex) and retrograde cavity preparation with ultrasonic tips were performed. Teeth were divided into 3 groups (n =10 each) following a stratified randomization according to the initial volume of the root-end cavity. After retrofilling, samples were stored for 7 days. Then, two rounds of micro-CT scans were performed: soon after root-end preparation (with the cavity still empty) and 7 days after root-end filling. Marginal gaps, internal voids volume (mm3 and %), as well as, the overall defects (sum of gaps and voids) were evaluated. Statistics compared the three groups in relation to those defects. There was not statistical difference between groups regarding the marginal gaps (P≥ 0.05), the internal voids (P≥ 0.05), and the overall defects (P≥ 0.05). Median (mm3) and % of overall air-entrapment defects (gaps and/or voids) was: 0.004mm3 and 1.749% for MTA Angelus, 0.018mm3 and 6.660% for Biodentine, and 0.012mm3 and 4.079% for Neo MTA Plus. All materials had gaps and/or voids. No differences were found between MTA Angelus, Biodentine and Neo MTA Plus.


Assuntos
Materiais Restauradores do Canal Radicular , Compostos de Cálcio , Humanos , Obturação do Canal Radicular , Tratamento do Canal Radicular , Silicatos , Microtomografia por Raio-X
13.
Braz Dent J ; 32(4): 8-18, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34787254

RESUMO

The aim of this study was to evaluate the physicochemical properties, cytocompatibility and antibiofilm activity of a new calcium silicate-based endodontic sealer, Sealer Plus BC (MK Life, Brazil), in comparison with TotalFill BC Sealer (FKG Dentaire SA, Switzerland) and AH Plus (Dentsply, Germany). Setting time and flow were evaluated based on ISO 6876 standard. The pH was evaluated after different periods, and radiopacity by radiographic analysis (mmAl). Solubility (% mass loss) and volumetric change (by micro-CT) were assessed after 30 days of immersion in distilled water. Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of Saos-2 cells to the sealer extract for 24 h. An additional analysis was performed by using MTT assay after 1, 3 and 7 days of exposure of Saos-2 to the sealers 1:8 dilution extracts. Antibiofilm activity against Enterococcus faecalis and/or Candida albicans was evaluated by crystal violet assay and modified direct contact test. The physicochemical properties were analyzed using ANOVA/Tukey tests; MTT and NR data were analyzed by ANOVA and Bonferroni tests; the antimicrobial tests were analyzed by Kruskal-Wallis and Dunn tests (α=0.05). Sealer Plus BC had proper setting time, radiopacity, flow and alkalization capacity. Sealer Plus BC was significantly more soluble than AH Plus (p<0.05) and presented volumetric change similar to AH Plus and TotalFill BC (p>0.05). Sealer Plus BC presented antibiofilm activity and no cytotoxic effect. In conclusion, although Sealer Plus BC had higher solubility, this sealer showed proper physicochemical properties, cytocompatibility, and antibiofilm activity.


Assuntos
Materiais Restauradores do Canal Radicular , Biofilmes , Compostos de Cálcio/farmacologia , Resinas Epóxi , Teste de Materiais , Materiais Restauradores do Canal Radicular/farmacologia , Silicatos/farmacologia
14.
Braz Oral Res ; 35: e117, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34816905

RESUMO

This study evaluated by the efficacy of rotary and reciprocating systems to remove filling material by micro-CT. Sixty human canines were instrumented up to F3 by ProTaper Universal system and filled with AH Plus/ gutta-percha. Specimens were submitted to 1,200 thermal cycles, scanned by microCT SkyScan 1176 and randomly distributed in groups according to the filling material removal protocol (n = 10): ProTaper Universal, ProTaper Universal/xylol, ProTaper Next, ProTaper Next/xylol, Reciproc and Reciproc/xylol. After filling removal, specimens were analyzed by microCT to separately verify the presence of gutta-percha and sealer remnants. The data (mm3) were analyzed by two way ANOVA and Tukey´s test (5%). For the remaining gutta-percha, the use of xylol provided the highest values for Reciproc (7.60 ± 0.73), which was statistically different from the other groups (p < 0.05) that presented lower values and were statistically similar to each other (p > 0.05). For the remaining sealer, xylol provided the highest values (21.25 ± 6.94) different (p < 0.05) from the use of instruments alone (11.47 ± 9.45). ProTaper Next presented the lowest values (8.16 ± 1.37) for the remaining sealer, different (p < 0.05) from that of Reciproc (24.67 ± 6.32). The qualitative analysis revealed that all groups presented some remaining root filling material, with the highest volumes presented by Reciproc and Reciproc/xylol. In conclusion the rotary systems provided greater removal of filling material regardless of the use of xylol. The use of xylol negatively interfered with the action of the reciprocating system in the removal of the gutta-percha and sealer.


Assuntos
Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Cavidade Pulpar/diagnóstico por imagem , Guta-Percha , Humanos , Retratamento , Preparo de Canal Radicular , Solventes , Microtomografia por Raio-X
15.
J Clin Pediatr Dent ; 45(5): 306-311, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34740264

RESUMO

INTRODUCTION: Bioceramic materials, gray and white mineral trioxide aggregate (GMTA, WMTA), have been shown to have high rates of success in various endodontic applications. A major drawback is their tendency to discolor teeth compared to Biodentine (BD), that has been claimed not to discolor teeth. The aim of this study was to compare tooth discoloration after applying different pulpotomy base materials (BD, GMTA and WMTA). STUDY DESIGN: Forty human incisors teeth were used in this study. Coronal access was achieved by a Tungsten Carbide drill, and the pulp chambers were accessed and chemo-mechanically debrided. Each material was placed in the pulp chamber, up to the cervical sectioning level. All specimens were incubated at 37°C and 100% humidity for three months and have been evaluated before the study and weekly. Color was assessed according to the CIE L*a*b* color space system. RESULTS: ΔE of all experimental groups (GMTA, WMTA and BD) were significantly different from the control group at all time points (P<0.05). Color changes in the GMTA and WMTA groups, had no statistically significant differences, but showed higher discoloration compared to BD group in the cervical part of the crown, since week 1 (P<0.05). WMTA group showed significant discoloration in the cervical part as of week 1 (P<0.05), and gradually increased over time (Figure 2). BD group showed no significantly discoloration over time. GMTA group showed the significant discoloration at week 1 and week 14 (P<0.05). CONCLUSIONS: both GMTA and WMTA pulpotomy materials may discolor tooth structure over time in an extracted permanent anterior tooth model. When choosing bioceramic pulpotomy material, BD may be preferable in esthetic area.


Assuntos
Materiais Restauradores do Canal Radicular , Descoloração de Dente , Compostos de Alumínio/efeitos adversos , Compostos de Cálcio/efeitos adversos , Coroas , Combinação de Medicamentos , Humanos , Incisivo , Óxidos/efeitos adversos , Pulpotomia , Silicatos/efeitos adversos , Coroa do Dente , Descoloração de Dente/induzido quimicamente
16.
Braz Dent J ; 32(3): 21-31, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34755787

RESUMO

This study assessed the fracture resistance of simulated immature teeth reinforced with calcium aluminate cement (CAC) or mineral trioxide aggregate (MTA) containing calcium carbonate nanoparticles (nano-CaCO3). The microstructural arrangement of the cements and their chemical constitution were also evaluated. Forty-eight canines simulating immature teeth were distributed into 6 groups (n=8): Negative control - no apical plug or root canal filling; CAC - apical plug with CAC; CAC/nano-CaCO3 - apical plug with CAC+5% nano-CaCO3; MTA - apical plug with MTA; MTA/nano-CaCO3 - apical plug with MTA+5% nano-CaCO3; and Positive control - root canal filling with MTA. The fracture resistance was evaluated in a universal testing machine. Samples of the cements were analyzed under Scanning Electron Microscope (SEM) to determine their microstructural arrangement. Chemical analysis of the cements was performed by Energy Dispersive X-ray Spectroscopy (EDS). The fracture resistance of CAC/nano-CaCO3 was significantly higher than the negative control (p<0.05). There was no significant difference among the other groups (p>0.05). Both cements had a more regular microstructure with the addition of nano-CaCO3. MTA samples had more calcium available in soluble forms than CAC. The addition of nano-CaCO3 to CAC increased the fracture resistance of teeth in comparison with the non-reinforced teeth. The microstructure of both cements containing nano-CaCO3 was similar, with a more homogeneous distribution of lamellar- and prismatic-shaped crystals. MTA had more calcium available in soluble forms than CAC.


Assuntos
Materiais Restauradores do Canal Radicular , Fraturas dos Dentes , Compostos de Alumínio , Compostos de Cálcio , Cimentos Dentários , Combinação de Medicamentos , Humanos , Óxidos , Obturação do Canal Radicular , Silicatos , Ápice Dentário
17.
Braz Dent J ; 32(3): 32-43, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34755788

RESUMO

The aim of this study was to evaluate the M1 and M2 macrophage modulation after stimuli with different materials used during endodontic treatment. In bone marrow-derived macrophage cell culture, from males C57BL/6 wild-type (WT) mice, gene expression analysis of markers to M1 and M2 macrophages was performed by qRT-PCR (Cxcl10, CxCL9, iNOS, Arg1, Chil3, Retnla and MRC1) and cytokine quantification by Luminex® (GM-CSF, IL-10, IL-6, IL-1ß and TNF-α) after exposure to the five endodontic sealers: AH Plus, Sealapex Xpress, Endosequence BC Sealer, BioRoot RCS and a calcium hydroxide-based paste. For normal values, ANOVA test was used, followed by Tukey post-test. For non-normal values, the Kruskall-Wallis test was used. BioRootTM RCS and EndoSequence BC SealerTM stimulated the highest expression of markers for M1 macrophages, while calcium hydroxide-based paste stimulated the lowest expression of these gene markers. For M2 protein markers, BioRootTM RCS presented the highest stimulation while calcium hydroxide-based paste also presented the lowest stimulation. It was concluded that all the evaluated filling materials increased the genetic expression of pro- and anti-inflammatory markers: TNF-α and IL-10 respectively. The others proinflammatory mediators showed differences against the filling materials. However, this process did not induce the inflammatory response polarization, resulting in a hybrid macrophage.


Assuntos
Materiais Restauradores do Canal Radicular , Animais , Resinas Epóxi , Macrófagos , Masculino , Teste de Materiais , Camundongos , Camundongos Endogâmicos C57BL , Fenótipo
18.
Braz Dent J ; 32(3): 65-74, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34755791

RESUMO

This study investigated the effect of three commercial calcium silicate-based materials (CSBM) on cytotoxicity and pro-and anti-inflammatory cytokines production in cultured human periodontal ligament stem cells (hPDLSCs). Culture of hPDLSCs was established and characterized. Extracts of Bio-C Sealer (Angelus, Londrina, PR, Brazil), MTA Fillapex (Angelus, Londrina, PR, Brazil) and PBS Cimmo HP (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brazil) were prepared by placing cement specimens (5 x 3 mm) in culture medium. Then, the extracts were serially two-fold diluted (1, 1:2, 1:4, 1:8, 1:16) and inserted into the cell-seeded wells for 24, 48 and 72 h for MTT assays. TNF-α and IL-10 cytokines were quantified by ELISA at 24h-cell supernatants. Data were analyzed by ANOVA and Tukey's test (α = 0.05). All CSBM exhibited some cytotoxicity that varied according to extract concentration and time of evaluation. MTA Fillapex presented the highest cytotoxic effects with significant reduction of metabolic activity/cell viability when compared to Bio-C Sealer and Cimmo HP®. TNF-α was significantly upregulated by the three tested cements (p < 0.05) while only MTA Fillapex significantly upregulated IL-10 in comparison to control. Taken collectively, the results showed that PBS Cimmo HP®, Bio-C Sealer and MTA Fillapex present mild and transient cytotoxicity and slightly induced TNF-α production. MTA Fillapex upregulated IL-10 release by hPDLSCs.


Assuntos
Compostos de Cálcio/efeitos adversos , Ligamento Periodontal , Materiais Restauradores do Canal Radicular/efeitos adversos , Silicatos/efeitos adversos , Células-Tronco/efeitos dos fármacos , Compostos de Alumínio , Citocinas/metabolismo , Humanos , Teste de Materiais , Óxidos , Ligamento Periodontal/citologia
19.
Indian J Dent Res ; 32(2): 221-225, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34810393

RESUMO

Background: Gutta-percha cones used directly from the manufacturer's sealed packs harbour micro-organisms. During clinical use, the chances of contamination of gutta-percha increases. Hence, disinfection of gutta-percha cones before use is essential to prevent canal recontamination. Aim: To evaluate the antimicrobial efficacy of babul against chemical disinfectants, and its effectiveness in disinfecting gutta-percha cones. Materials and Methods: The extract of babul was used to assess its antimicrobial activity against E-faecalis, S. aureus and C. albicans using the agar diffusion method. A total of 120 cones acquired from freshly opened manufacturers' pack were cut into three equal parts and divided into group I which was directly placed into a nutrient broth, group II was clinically contaminated with gloves for 30 s and placed into a nutrient broth, and the third part was clinically contaminated with gloves and decontaminated for 5 min using the experimental disinfectants to check for the presence of turbidity. Statistical Analysis: The data were analysed by an Unpaired t-test and Chi-square test at a significance level of P < 0.05 using software SPSS 20. Results: Two percent chlorhexidine showed maximum antibacterial action against E. faecalis and S. aureus followed by babul extract. It was also found more efficacious in decontaminating gutta-percha cones followed by babul and 3% sodium hypochlorite in 5 min. Conclusion: Babul herbal extract is indeed effective in decontaminating gutta-percha cones and is a better alternative to chemical disinfectants.


Assuntos
Acacia , Anti-Infecciosos , Materiais Restauradores do Canal Radicular , Antibacterianos , Guta-Percha/farmacologia , Extratos Vegetais/farmacologia , Staphylococcus aureus
20.
Scanning ; 2021: 8726388, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34737843

RESUMO

This study evaluated the effects of different final irrigation regimens on the dentin tubule penetration of three different root canal sealers using confocal laser scanning microscopy (CLSM). A total of 160 single-rooted extracted mandibular premolar teeth were divided into five groups (n = 32 each) according to the solution used in the final rinse protocol, as follows: 17% ethylenediaminetetraacetic acid (EDTA) (group 1), 17% EDTA and 2% chlorhexidine gluconate (CHX) (group 2), 7% maleic acid (MA) (group 3), 7% MA and 2% CHX (group 4), and 5.25% NaOCl (group 5). Two roots from each group were examined under scanning electron microscopy (SEM) to visualize smear layer removal. Experimental groups were then split randomly into three subgroups (n = 10) and obturated using a cold lateral condensation technique with 0.1% rhodamine B-labelled sealers [either AH Plus (group A), EndoREZ (group E), or Tech BioSealer Endo (group T)] and gutta-percha. Specimens were sectioned and observed by CLSM to evaluate the percentage and maximum depth of sealer penetration at the apical, middle, and coronal levels. Statistical comparison was performed on grouped (apical, middle, and coronal segments) and ungrouped data using two-way ANOVA with Bonferroni post hoc test (p < 0.01). AH Plus showed the greatest tubule penetration while Tech BioSealer Endo showed the least. Resin-based sealers displayed deeper and more consistent penetration. CHX irrigation positively influenced sealer tubule penetration.


Assuntos
Materiais Restauradores do Canal Radicular , Cavidade Pulpar , Dentina , Resinas Epóxi , Humanos , Microscopia Confocal , Microscopia Eletrônica de Varredura , Materiais Restauradores do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/farmacologia , Preparo de Canal Radicular
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