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1.
Artigo em Inglês | MEDLINE | ID: mdl-31697202

RESUMO

Chloramphenicol (CAP) is a broad-spectrum antibiotic used widely both in human and in veterinary medication but due to adverse health effects is not authorised anymore for use in food-producing animals in many countries. CAP molecule contains two asymmetric centers resulting in four para-CAP stereoisomers, but only the RR-CAP enantiomer is bioactive with significant antimicrobial activity. In this study the detection of the four CAP stereoisomers was tested by five commercial ELISA kits and the Charm® II Chloramphenicol Test. These immunoassay tests are commonly used and widely accepted for screening of CAP residues in foods of animal origin, including honey. The test results definitely show that SS-CAP residues are not detected; even high SS-CAP concentrations are missed due to the lack of any cross reactivity and the high specificity of the CAP antibodies to RR-CAP. In former studies chiral LC-MS/MS analysis indicated clearly that honey samples with raised CAP concentrations often contain the SS-CAP enantiomer in addition to the bioactive RR-CAP. According to this study, the investigated screening tests carry the risk of systematically false-compliant (false negative) results for CAP and a discrepancy between LC-MS/MS and ELISA/Charm® test results. As a consequence of this study, it is recommended that immunoassay manufacturers develop and use CAP antibodies which also bind SS-CAP. The origin of SS-CAP residues in honey samples is discussed and general toxicological and regulatory aspects of CAP stereoisomers are raised.


Assuntos
Cloranfenicol/análise , Resíduos de Drogas/análise , Ensaio de Imunoadsorção Enzimática , Contaminação de Alimentos/análise , Mel/análise , Cromatografia Líquida , Reações Falso-Negativas , Estrutura Molecular , Fatores de Risco , Estereoisomerismo , Espectrometria de Massas em Tandem
2.
Anal Bioanal Chem ; 412(2): 463-472, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31768593

RESUMO

Honey is one of the food commodities most frequently affected by fraud. Although addition of extraneous sugars is the most common type of fraud, analytical methods are also needed to detect origin masking and misdescription of botanical variety. In this work, multivariate analysis of the content of certain macro- and trace elements, determined by energy-dispersive X-ray fluorescence (ED-XRF) without any type of sample treatment, were used to classify honeys according to botanical variety and geographical origin. Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to create classification models for nine different botanical varieties-orange, robinia, lavender, rosemary, thyme, lime, chestnut, eucalyptus and manuka-and seven different geographical origins-Italy, Romania, Spain, Portugal, France, Hungary and New Zealand. Although characterised by 100% sensitivity, PCA models lacked specificity. The PLS-DA models constructed for specific combinations of botanical variety-country (BV-C) allowed the successful classification of honey samples, which was verified by external validation samples. Graphical abstract.


Assuntos
Geografia , Mel/classificação , Modelos Químicos , Espectrometria por Raios X/métodos , Análise Discriminante , Europa (Continente) , Mel/análise , Limite de Detecção , Análise Multivariada , Reprodutibilidade dos Testes
3.
Bull Environ Contam Toxicol ; 104(1): 84-89, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31807795

RESUMO

Element concentrations were measured in multifloral honeys sampled from Central and Eastern Croatia. The mean levels of elements ranged from (µg/kg): Al 323-7228, Cu 103-1033, Cr 14.4-139, Fe 295-2336, Ni 122-523, Pb 9.65-154, Zn 442-2025. In all samples, As and Cd content were below the LOD values. Significant differences in the concentrations of Al, Cr, Cu, Fe, Pb and Zn (p < 0.01) were found in honeys from different locations within regions and within locations of each region. Also, significant differences in total element contents between the two regions were determined for Cr and Cu (p < 0.01). No significant differences were observed in total Al, Fe, Pb and Zn levels between regions. The highest Al, Cr, Cu, Fe and Zn concentrations were measured in Central Croatia, while Ni and Pb in Eastern Croatia. The results confirm the decisive influence of collection location on the composition of toxic and trace elements in honey.


Assuntos
Monitoramento Ambiental , Poluentes Ambientais/análise , Substâncias Perigosas/análise , Mel/análise , Oligoelementos/análise , Croácia
4.
J Sci Food Agric ; 100(3): 1030-1037, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31646633

RESUMO

BACKGROUND: The objective of this work was to develop gummy jellies containing honey and propolis, keeping the natural antioxidant principles of the beehive ingredients and satisfying consumer requirements. RESULTS: A gummy jelly containing honey and propolis (HPGJ) was developed. A sensory study with consumers (n = 74) performed an intensity level evaluation test analyzing color, hardness, adhesiveness, gumminess, sweet taste and honey taste. A penalty analysis indicated that HPGJ was too hard. However, in the global acceptance study, more than 90% of consumers gave liking categories. Regarding the potential functional properties, the antioxidant capacity (AC) of HPGJ was 8.17 ± 0.55 mmol Trolox equivalent kg-1 , and up to 40% AC was retained after in vitro digestion. Additionally, AC of HPGJ was up to ten times higher than that of similar commercial products. A storage study at 25 °C showed that color and AC increased significantly over 90 days owing to the development of Maillard reaction. Storage under darkness allowed keeping low values of global color change for up to 45 days. Another positive facet was that the addition of propolis delayed fungal growth during storage. CONCLUSION: An organoleptically palatable gummy jelly was obtained. Among the positive features, it showed higher AC than similar commercial candies. Additionally, HPGJ offered a high bioaccesible AC input detected upon in vitro digestion. Overall, HPGJ could be considered an interesting, appetizing and healthier alternative to regular gummy jellies available in the market. Adequate packaging should be considered in order to extend HPGJ shelf life, reducing browning reactions. © 2019 Society of Chemical Industry.


Assuntos
Doces/análise , Mel/análise , Própole/análise , Antioxidantes/análise , Manipulação de Alimentos , Armazenamento de Alimentos , Géis/análise , Humanos , Reação de Maillard , Paladar
5.
J Sci Food Agric ; 100(5): 1913-1921, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31846080

RESUMO

BACKGROUND: Invert syrup is a common adulterant in honey falsification, thus generating risk for consumers. Most of the methods developed are tedious and time-consuming for manufactures and consumers. However, terahertz spectroscopy provides analytical information in a simple, rapid, and environmentally friendly manner. Subsequently, 3 kinds of terahertz spectroscopic characteristics data, the absorption coefficient, the slope of the absorption coefficient spectra, and the area of the absorption coefficient spectra, were employed for determination of acacia honey adulterated with invert syrup. RESULTS: Single linear regression (SLR) models with different terahertz spectroscopic features were adopted to predict the syrup adulterant proportion in acacia honey. The best SLR model used the area of the absorption coefficient, displaying an adjusted correlation coefficient of 0.985 and a root-mean-square error of 3.201. Meanwhile, multiple linear regression (MLR) models using a successive projections algorithm for variables selection were implemented. The MLR model considered the integral area of the absorption coefficient spectra, as the inputs yielded the best result with less variables selected, higher R c 2 and R p 2 , lower root-mean-square error of calibration and prediction, as well as higher residual predictive deviation. CONCLUSIONS: The results indicate terahertz spectroscopy combined with the integral area of the absorption coefficient spectra is reliable enough for invert syrup proportion quantification in acacia honey and is also a rapid and nondestructive determination method for other honey adulterants. © 2019 Society of Chemical Industry.


Assuntos
Acacia/química , Contaminação de Alimentos/análise , Mel/análise , Espectroscopia Terahertz/métodos , Modelos Lineares , Análise Multivariada , Espectroscopia Terahertz/instrumentação
6.
Food Chem ; 309: 125685, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31693964

RESUMO

A doubly porous microcomposite polyaniline/graphene oxide/octadecyl-bonded silica magnetite (PANI/GOx/C18-SiO2-Fe3O4) alginate adsorbent was developed and employed to extract fluoroquinolones. The Fe3O4 facilitated rapid and convenient for the separation of the adsorbent from sample solutions. The double porosity of the alginate hydrogel enhanced the surface area of the polyaniline coating. The developed method exhibited good linearity of 0.0010-50 µg L-1 for danofloxacin; 0.0050-50 µg L-1 for norfloxacin, ciprofloxacin and enrofloxacin; and 0.010-50 µg L-1 for sarafloxacin and difloxacin. The limits of detection were between 0.001 and 0.010 µg L-1 with RSD below 9.0%. The PANI/GOx/C18-SiO2-Fe3O4 adsorbent was utilized to extract fluoroquinolones from honey, milk and egg samples and satisfactory extraction recoveries were achieved ranged from 80 to 98%. The developed adsorbent has good stability which can be reused up to 7 times, is simple to prepare and convenient to use for the extraction fluoroquinolones.


Assuntos
Fracionamento Químico/métodos , Fluoroquinolonas/isolamento & purificação , Análise de Alimentos , Compostos de Anilina/química , Animais , Ciprofloxacino/análogos & derivados , Ciprofloxacino/isolamento & purificação , Ovos/análise , Enrofloxacina/isolamento & purificação , Óxido Ferroso-Férrico/química , Grafite/química , Mel/análise , Limite de Detecção , Leite/química , Norfloxacino/isolamento & purificação , Porosidade , Dióxido de Silício/química
7.
Chemosphere ; 239: 124809, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31527008

RESUMO

Honey is a highly nutritious natural product widely produced and consumed by people in Shirak and Syunik regions of Armenia. Unlike Shirak, Syunik is under the impact of mining industry. Since the environmental pollution can adversely impact the safety of honey and entail a probable risk to human health, it is important to evaluate the presence of potentially toxic trace elements in honey samples from both regions and draw comparisons. This study assesses the dietary exposure to trace elements and persistent organic pollutants through the intake of honey for the first time among people in Shirak and Syunik regions. 24-hour dietary recall method was used to investigate the consumption of honey. The presence of seven trace elements (Pb, Cd, As, Hg, Cu, Zn, and Ni) and persistent organic pollutants (hexachlorocyclohexane (HCH), dichlorodiphenyltrichloroethane (DDT) and its metabolites) were determined in honey samples using atomic absorption spectrometry and gas chromatography-mass spectrometry, respectively. In several samples, the concentrations of Cu were above the maximum allowable level. Non-carcinogenic risk values did not exceed the acceptable level, while carcinogenic risk values for Ni and As exceeded the risk level of 10-6 in both regions. Moreover, among the persistent organic pollutants, only the concentration of DDT in honey from Shirak was above the European Union maximum residue level.


Assuntos
Carcinógenos/análise , DDT/análise , Exposição Dietética/análise , Mel/análise , Medição de Risco/métodos , Oligoelementos/análise , Armênia , Poluentes Ambientais/análise , Poluição Ambiental/análise , Mel/toxicidade , Humanos , Metais Pesados/análise , Mineração , Espectrofotometria
8.
Spectrochim Acta A Mol Biomol Spectrosc ; 224: 117415, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31374352

RESUMO

A simple fluorescent strategy based on the formation of triple-helix molecular switch (THMS) between a signal transduction probe (STP) and an aptamer (Apt) was constructed for the determination of chloramphenicol (CAP). A weak fluorescence intensity was observed for STP solution due to the proximity of fluorophore and quencher through intramolecular DNA hybridization, causing the fluorescence quenching. The fluorescence intensity of the system was significantly enhanced after the addition of Apt. It was attributed to the formation of THMS between the Apt and STP through the Watson-Crick and Hoogsteen base pairing, resulting in the restoration of fluorescence because of the long distance between the fluorophore and quencher of STP. The fluorescence intensity of the system decreased due to the release of STP caused by the specific binding between Apt and CAP. The quantitative analysis of CAP could be achieved based on the decreased fluorescence intensity. The parameters affecting the performance of THMS including the Apt arm length, pH of buffer solution, Mg2+ concentration and the formation time of THMS were investigated in detail. Under the optimal conditions (Apt arm length of 9 bases, pH of 6.5, 2.5 × 103 µmol L-1 Mg2+, THMS formation time of 30 min), the decreased fluorescence intensity and the concentration of chloramphenicol were linear in the range of 5.0 × 10-3-2.0 × 10-1 µmol L-1 with the correlation coefficient of 0.9963. The limit of detection was 1.2 nmol L-1. Subsequently, the developed method was applied to the analysis of chloramphenicol in honey sample, and the recovery was between 84.5% and 103.0% with relative standard deviation less than 4.6%.


Assuntos
Cloranfenicol/análise , Corantes Fluorescentes/química , Espectrometria de Fluorescência/métodos , Aptâmeros de Nucleotídeos/química , Resíduos de Drogas/análise , Mel/análise , Limite de Detecção , Modelos Lineares , Hibridização de Ácido Nucleico , Reprodutibilidade dos Testes
9.
Food Chem ; 309: 125788, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31753683

RESUMO

The sugar profile in honey can be used as a fingerprint to confirm the authenticity or reveal the adulteration of the product by sweetener addition. In this work, we have accurately determined the profile of 20 minor saccharides in a set of 46 European acacia honeys using a recently proposed NMR approach based on the CSSF-TOCSY experiment. Comparison of this reference profile with the sugar composition of several Chinese honey samples of the same declared botanical origin has revealed important differences. A detailed analysis of the saccharide profile of these Chinese honeys suggests product adulteration by overfeeding bee colonies with industrial sugars syrups during the main nectar flow period.


Assuntos
Acacia/química , Mel/análise , Espectroscopia de Ressonância Magnética , Açúcares/química , Acacia/metabolismo , Animais , Abelhas , China , Contaminação de Alimentos/análise , Análise de Componente Principal , Açúcares/análise
10.
Food Chem ; 309: 125656, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31699558

RESUMO

The adulteration of honey is generally a safety and quality concern for consumers and the industry as a whole. Resin technologies allow harmful substances to enter honey, creating substandard honey, which can enter the market. Thus, it is necessary to identify such illegal products quickly and easily. In this study, HPLC-ECD combined with chemometrics was used to identify raw acacia honey that had been treated with macroporous adsorption resins. The chromatography fingerprints of 46 honey samples were established, and principal component analysis (PCA) and the OPLS-DA identified that differences in some of the chromatographic peaks could be used to distinguish raw from resins-treated raw honeys. 100% correct classification was achieved with test samples, based on the chromatographic peaks identified. These results show that HPLC-ECD, combined with chemometric methods, can identify correctly resins-treated honey and can be applied for the quality control of honey.


Assuntos
Acacia/química , Cromatografia Líquida de Alta Pressão/métodos , Mel/análise , Resinas Vegetais/química , Acacia/metabolismo , Adsorção , Análise Discriminante , Análise dos Mínimos Quadrados , Porosidade , Análise de Componente Principal
11.
Food Chem ; 305: 125511, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31610421

RESUMO

Honey spirit is an alcoholic beverage produced by fermentation followed by distillation of the honey must, which has distinct organoleptic characteristics derived mostly from the raw material used. In order to accurately monitor the quality of the product throughout the distillation process (head, heart and tail stages), FT-RAMAN spectroscopy was applied. Dark honey, light honey and honey obtained following waxes' wash was used to produce honey spirit. The pH, alcoholic strength, methanol content, acetaldehyde content, ethyl acetate content and higher alcohols content were evaluated during the distillation process. The FT-RAMAN technique was used to obtain spectral information for all fractions collected during beverage production. The results suggest that the honey spirit had good quality concerning the volatile composition and methanol was not detected in any sample. FT-RAMAN is promising for the online monitoring of the distillation process in order to improve the final quality of this beverage.


Assuntos
Bebidas Alcoólicas/análise , Destilação/métodos , Mel/análise , Análise Espectral Raman , Qualidade dos Alimentos
12.
Food Chem ; 306: 125595, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31610324

RESUMO

Assessing the authenticity of honey is a serious problem that has gained much interest internationally because honey has frequently been subject to various fraudulent practices, including mislabelling of botanical and geographical origin and mixing with sugar syrups or honey of lower quality. To protect the health of consumers and avoid competition, which could create an unstable market, consumers, beekeepers and regulatory bodies are interested in having reliable analytical methodologies to detect non-compliant honey. This paper gives an overview of the different approaches used to assess the authenticity of honey, specifically by the application of advanced instrumental techniques, including spectrometric, spectroscopic and chromatographic methods coupled with chemometric interpretation of the data. Recent development in honey analysis and application of the honey authentication process in the Romanian context are highlighted, and future trends in the process of detecting and eliminating fraudulent practices in honey production are discussed.


Assuntos
Mel/análise , Carboidratos/análise , Cromatografia , Contaminação de Alimentos/análise , Romênia , Análise Espectral
13.
Food Chem ; 305: 125457, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505414

RESUMO

Taking into account a growing market and small number of articles related to honeydew honey, a metabolomic approach associated with multivariate analysis and modelling was proposed to discriminate five varieties of honey. Advanced analytical techniques were used for determination of 20 elements, 14 carbohydrates and stable carbon isotope ratio. No chemical marker has been found within sugar compounds, but several elements (Ba, Ca, Mg, Sr, Mn, Al, Co, Ni, Se) were marked as characteristic of honey type and allow classification of three botanical origins (Abies alba, Quercus frainetto, Quercus ilex). Sugars turanose, trehalose, arabinose and raffinose, elements Ba, Sr, P, Cd and Se, and δ13C values of honey, have different concentrations in honeys of the same botanical origin but harvested in different season. In addition to a confirmation of authenticity in terms of production, the values of δ13C of protein could be a good indicator of botanical origin.


Assuntos
Mel/análise , Espectrometria de Massas/métodos , Quercus/metabolismo , Isótopos de Carbono/química , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Análise Discriminante , Análise dos Mínimos Quadrados , Proteínas de Plantas/análise , Proteínas de Plantas/isolamento & purificação , Análise de Componente Principal , Álcoois Açúcares/análise , Açúcares/análise
14.
Chemosphere ; 238: 124555, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31454746

RESUMO

Pesticide residues in bee products is still a major issue. However, the relations to botanical source and land use characteristics are not clear. The large variability of residues detected questions the suitability of bee-collected- and other hive materials as indicators for environmental contamination. The aim of our study was to clarify whether different beehive matrices contain similar pesticide residues, and how these are correlated with forage preferences and land use types in foraging areas. We tested bee-collected pollen, beebread, honey, nurse bees and honey bee larvae for the presence of concurrently used agricultural pesticides in Estonia. Samples were collected at the end of May and mid-July to include the main crop in northern region - winter and spring oilseed rape (Brassica napus). We saw that different beehive matrices contained various types of pesticide residues in different proportions: pollen and beebread tended to contain more insecticides and fungicides, whereas herbicides represented the primary contaminant in honey. The variations were related to collection year and time but were not related to crops as basic forage resource nor the land use type. We found few positive correlations between amount of pesticides and proportion of pollen from any particular plant family. None of these correlations were related to any land-use type. We conclude that pesticide residues in different honey bee colony components vary largely in amount and composition. The occurrence rate of pesticide residues was not linked to any particular crop.


Assuntos
Abelhas/efeitos dos fármacos , Brassica napus/crescimento & desenvolvimento , Produtos Agrícolas/crescimento & desenvolvimento , Mel/análise , Resíduos de Praguicidas/análise , Pólen/química , Animais , Brassica napus/efeitos dos fármacos , Produtos Agrícolas/efeitos dos fármacos , Estônia , Estações do Ano
15.
J Sci Food Agric ; 100(1): 212-217, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31487046

RESUMO

BACKGROUND: The growing need to classify the origin of honey in a simple way is leading to the development of affordable analytical equipment that is in-line and manageable, enabling rapid on-site screening. The aim of this work was therefore to evaluate whether an electronic tongue (made of four metallic electrodes: Ir, Rh, Pt, Au), based on potential multistep pulse voltammetry with electrochemical polishing, is able to differentiate between honey samples from Spain, Honduras, and Mozambique. RESULTS: It was demonstrated, for the first time, that automatic pulse voltammetry, in combination with principal component analysis (PCA) statistical analysis, was able to differentiate honey samples from these three countries. A partial least squares (PLS) analysis predicted the level of certain physicochemical parameters, the best results being for conductivity and moisture with correlation coefficients of 0.948 and 0.879, whereas the weakest correlation was for the sugars. CONCLUSION: The tool proposed in this study could be applied to identify the country origin of the three types of multifloral honey considered here. It also offers promising perspectives for expanding knowledge of the provenance of honey. All of this could be achieved when a comprehensive database with the information generated by this electronic tongue has been created. © 2019 Society of Chemical Industry.


Assuntos
Nariz Eletrônico , Mel/análise , Análise Discriminante , Condutividade Elétrica , Contaminação de Alimentos , Honduras , Mel/classificação , Análise dos Mínimos Quadrados , Moçambique , Análise de Componente Principal , Espanha
16.
J Sci Food Agric ; 100(1): 335-342, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31584691

RESUMO

BACKGROUND: Antibacterial activity of honey is not only crucial characteristic in selection of honey for medical usage but also an important honey quality marker. The aim of the study was to characterise the antibacterial potential of 29 honey samples representing the main types of multi-floral blossom and honeydew honeys produced in Switzerland. Antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa was expressed as a minimum inhibitory and bactericidal concentrations (MIC and MBC). Furthermore, the content of bee-derived glucose oxidase (GOX) and its enzymatic product, H2 O2 , were also evaluated. RESULTS: All honey samples successfully met basic defined criteria (moisture and hydroxymethylfurfural (HMF)) tested in this study. Honeydew honeys were the most effective honey samples and generated the highest levels of H2 O2 . A strong significant correlation was found between the overall antibacterial activity and the level of H2 O2 among all honey samples. Interestingly, the content of GOX in honey samples did not correlate with their antibacterial activity as well as H2 O2 production capacity. A weak antibacterial activity was determined in five floral honeys, most likely due to increased enzymatic activity of pollen-derived catalase. CONCLUSION: This study showed that antibacterial effect of Swiss honey samples is associated mainly with H2 O2 . © 2019 Society of Chemical Industry.


Assuntos
Antibacterianos/análise , Mel/análise , Animais , Antibacterianos/farmacologia , Abelhas , Catalase/análise , Glucose Oxidase/análise , Mel/classificação , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Suíça
17.
J Agric Food Chem ; 67(46): 12885-12894, 2019 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-31675227

RESUMO

α-Dicarbonyl compounds (α-DCs) are a major class of intermediates generated during Maillard reactions. They can serve as chemical markers of thermal processing and storage of sugar-rich foods. To distinguish between naturally matured acacia honey (NMAH) and artificially heated acacia honey (AHAH), we purified 12 major α-DCs quinoxaline derivatives to investigate the effects of temperature during heat treatment and storage on their accumulation in acacia honey. Nine of the 12 α-dicarbonyl compounds were found in acacia honey samples, and their contents varied depending on processing and storage conditions. Among them, the contents of 3-deoxyglucosulose (3-DG), 1,4-dideoxyglucosone (1,4-DDG), and 1-deoxyglucosone (1-DG) increased commensurately with heat. 3-DG content ranged from 103.7 to 146.6 mg/kg in NMAH and 572.4-1371.2 mg/kg in AHAH. Given the abundance and stability of 3-DG following heat treatment and storage, this compound can potentially serve as a reliable marker for distinguishing between NMAH and AHAH.


Assuntos
Acacia/química , Mel/análise , Manipulação de Alimentos , Temperatura Alta , Reação de Maillard , Controle de Qualidade
18.
J Agric Food Chem ; 67(43): 12144-12152, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31587558

RESUMO

Fraudulent acts regarding honey authenticity that use Apis mellifera honey as a substitute for Apis cerana honey have garnered considerable concern in China and triggered a trust crisis from consumers. In this study, untargeted metabolomics analysis was carried out based on volatile fractions in honey from A. cerana and A. mellifera using headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS). Honey from A. cerana and A. mellifera was discriminated by HS-GC-IMS profiling, principal component analysis, and orthogonal partial least-squares discrimination analysis. Tentative markers were identified from p-values and the variable importance in projection analysis and confirmed using the retention index, mass fragments, and reference standards by gas chromatography-mass spectrometry (GC-MS). A targeted method was established using the headspace solid phase coupled with microextraction GC-MS (HS-SPME-GC-MS) to quantitate the markers. The results demonstrated that the developed untargeted and targeted metabolomics approach performed well when discriminating honey from A. cerana and A. mellifera.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Mel/análise , Microextração em Fase Sólida/métodos , Compostos Orgânicos Voláteis/química , Compostos Orgânicos Voláteis/isolamento & purificação , Animais , Abelhas/classificação , China , Análise Discriminante , Mel/classificação , Espectrometria de Mobilidade Iônica/métodos
19.
J Agric Food Chem ; 67(44): 12255-12263, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31618580

RESUMO

Jujube (Ziziphus jujuba Mill.) honey, one of the most valuable honey varieties from China with unique characteristics, is vulnerable to being the target of adulteration and deliberate mislabeling of botanical origin. This study investigated the typical protein component of jujube honey to authenticate the floral source by SDS-PAGE analysis combined with LC-MS/MS identification, and its stability to heating was also evaluated. One band and two adjacent but independent bands, both with molecular weights of ∼19 kDa, were notably observed in Coomassie brilliant blue- and silver-stained SDS-PAGE gels, respectively, for jujube honey from different geographic origins, whereas that was not present for the other five botanical honey varieties, suggesting this protein component was suitable as a marker for jujube honey. LC-MS/MS identification revealed that it was constituted by one Z. jujuba-derived protein (gene number:Zj.jz016003045) and two A. mellifera-derived proteins (an uncharacterized protein with accession number tr|A0A088AC16 and a cleavage fragment from major royal jelly protein-1), and the existence of plant-derived protein was attributed to the special neutral pH of jujube honey. Additionally, these protein markers exhibited good stability to heating below 85 °C/30 min. This study provided a simple method to characterize jujube honey and first identified a protein indicator to determine the botanical origin of honey.


Assuntos
Proteínas de Plantas/química , Ziziphus/química , Sequência de Aminoácidos , China , Cromatografia Líquida , Eletroforese em Gel de Poliacrilamida , Frutas/química , Frutas/genética , Mel/análise , Peso Molecular , Proteínas de Plantas/genética , Espectrometria de Massas em Tandem , Ziziphus/genética
20.
J Agric Food Chem ; 67(40): 11256-11261, 2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31545583

RESUMO

Honey is a natural product that could be easily adulterated with various cheaper sweeteners. In the present study, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied for the detection of honey adulteration based on oligosaccharide and polysaccharide profiles. MS-based strategy could reveal the presence of polysaccharides with higher degree of polymerization (DP ≥ 13) and abnormal trends of saccharides in adulterated honey samples, which could be used as indicators for the identification of honey adulteration with high-fructose corn syrup and corn syrup. MS/MS-based strategy was proposed to characterize the difference in the composition of oligosaccharide isomers between honey samples and adulterated ones with corn syrup or invert syrup, in which the [M+Cl]- of disaccharides, trisaccharides, and tetrasaccharides were fragmented to give diagnostic product ion pairs. The method is effective and robust for the high-throughput monitoring of honey adulteration, and provides a new perspective for the identification of other high-carbohydrate foods.


Assuntos
Contaminação de Alimentos/análise , Mel/análise , Oligossacarídeos/análise , Polissacarídeos/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Xarope de Milho Rico em Frutose/análise , Espectrometria de Massas em Tandem/métodos
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