RESUMO
The sudden death of three calves, one diarrheic calf, and one aborted fetus from four farms in southern Brazil was investigated. Two Histophilus somni-associated syndromes were identified: systemic histophilosis (n = 4) and abortion (n = 1). The principal pathological findings included vasculitis, meningoencephalitis with thrombosis, necrotizing myocarditis, renal infarctions, hepatic abscesses, and bronchopneumonia. PCR assays were used to amplify specific amplicons of the ovine herpesvirus 2, bovine herpesvirus 1 and -5, Listeria monocytogenes, H. somni, and pestivirus; bovine group A rotavirus (BoRV-A) and bovine coronavirus (BCoV) were investigated in calves with diarrhea. H. somni DNA was amplified in tissues from all calves and the brain of the aborted fetus with pathological alterations consistent with histophilosis. All other PCR assays were negative; BoRV-A and BCoV were not identified. These findings confirm the participation of H. somni in the pathological alterations observed in this study and represent the first description of histophilosis in cattle from Brazil.
Assuntos
Aborto Animal/patologia , Doenças dos Bovinos/patologia , Infecções por Haemophilus/veterinária , Haemophilus somnus/genética , Meningite por Haemophilus/veterinária , Aborto Animal/microbiologia , Animais , Brasil , Bovinos , Doenças dos Bovinos/microbiologia , Infecções por Haemophilus/complicações , Infecções por Haemophilus/microbiologia , Infecções por Haemophilus/patologia , Haemophilus somnus/isolamento & purificação , Haemophilus somnus/metabolismo , Meningite por Haemophilus/microbiologia , Meningite por Haemophilus/patologia , Dados de Sequência Molecular , Filogenia , Reação em Cadeia da Polimerase/veterinária , RNA Bacteriano/genética , RNA Bacteriano/metabolismo , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterináriaRESUMO
This work describes a case of Haemophilus influenzae serotype a meningitis in Brazil, after almost a decade since the introduction of Haemophilus influenzae serotype b conjugate vaccine. Uncertainty about the replacement of H. influenzae serotypes as a cause of invasive diseases justifies continuous surveillance, coupled with investigations of carriage rates and requirements of chemoprophylaxis in contact persons.
Assuntos
Haemophilus influenzae/classificação , Meningite por Haemophilus/epidemiologia , Brasil/epidemiologia , Feminino , Humanos , Lactente , Meningite por Haemophilus/líquido cefalorraquidiano , Meningite por Haemophilus/patologia , SorotipagemRESUMO
Computed tomography has been applied to childhood bacterial meningitis in an attempt to analyze the structural basis for neurologic complications. The CT findings in meningitis patients include acute cerebral swelling; moderate widening of basal cisterns, interhemispheric fissue, and subarachnoid convexity space; ventricular widening; subdural collection; focal cortical necrosis; cerebral infarcts; contrast enhancing basal meninges, ependymitis, and generalized cerebral atrophy. Bacterial meningitis in childhood is more than an inflammation of the subarachnoid space. It is a disease process which often exerts a profound effect on the brain parenchyma.