Biogeographic distribution, assembly processes and potential nutrient cycling functions of myxobacteria communities in typical agricultural soils in China.

Predatory myxobacteria are important soil micropredators with the potential to regulate soil microbial community structure and ecosystem function. However, the biogeographic distribution patterns, assembly processes, and potential nutrient cycling functions of myxobacteria communities in typical agricultural soils in China are still poorly understood. High-throughput sequencing, phylogenetic zero modeling, and the multi-nutrient cycling index were used to assess the biogeographic distribution, assembly processes, and soil ecosystem functions of predation myxobacteria communities in typical agricultural soils of six long-term fertilization ecological experimental stations. The results demonstrated a hump-shaped distribution of myxobacteria α-diversity along the latitudinal gradient and significant differences in myxobacteria ß-diversity in typical agricultural soils (P < 0.05). Bacterial richness, soil organic carbon, and pH were the most important predictors of myxobacteria α-diversity, whereas geographic factors and soil pH were the most significant ecological predictors of myxobacteria ß-diversity. Myxobacteria community assembly is dominated by deterministic processes, especially homogeneous selection, primarily driven by soil pH and bacterial richness. In addition, we revealed the ecological significance of myxobacteria communities in typical agricultural soil microbial networks and the potential link between myxobacteria communities and soil nutrient cycling. These findings enhance our understanding of the biogeographic distribution, community assembly, ecological predictors, and relationships with soil nutrient cycling of myxobacteria communities in typical agricultural soils, paving the way for a more predictive understanding of the effect of predatory myxobacteria communities on soil ecosystem function, which is essential for the development of sustainable agriculture.

The mechanism of microbial community succession and microbial co-occurrence network in soil with compost application.

The application of organic and chemical fertilizer into soil can regulate microbial communities. However, the response mechanism of microbial communities in soil to compost and chemical fertilizer application remain unclear. In this study, compost made of tobacco leaves individually and combined with chemical fertilizer was applied, respectively, to investigate their effect on soil microorganisms during the pot-culture process. High-throughput sequence, neutral community model and null model were employed to clarify how soil microbial community respond to the application of compost and chemical fertilizer. Furthermore, random forest model was applied to predict the relationships between the plant agronomical traits and the soil microorganism during the pot-culture process. The results demonstrated that the simultaneous application of compost and chemical fertilizer increased significantly the richness and diversity of the microorganisms in soil (p < 0.05), groups C and D led to a significant reduction in the number of nodes and edges in the microbial network (77.78 %-96.57 %). The dominant bacteria in the application of 50 g fertilizer accounted for the highest proportion (40 %) and organic matter was the main factors driving the change in bacterial communities. Compared to the tilled soil, the microbial communities of the soil with the simultaneous application of compost and chemical fertilizer were more susceptible to stochastic processes, and soil microorganisms had less influence on the growth of crops during pot-culture. In conclusion, the simultaneous application of compost and fertilizer altered the ecological functions of soil microbial communities, leading to an enhanced stochastic process of community formation.

Tomato growth and physiology as well as soil physicochemical and biological properties affected by ozonated water in a saline agroecosystem.

Current trends in agriculture are focused on implementing sustainable practices that avoid the use of chemical compounds. It is important to propose environmentally friendly methods, which may enhance plant growth physiology and yield without affecting soil microbial community as much. In this context, irrigation with ozonated water could be a potential strategy to reduce some chemical compounds in soils due to the degradative power of ozone. Here, we studied the impact of irrigation with ozonated water on the microbial community of a Mediterranean soil, and on Solanum lycopersicum L. agro-physiology and productivity in a greenhouse experiment. To this end, we evaluated: i) soil physicochemical properties, soil enzyme activities, microbial biomass via fatty acid analysis, microbial diversity (via amplicon sequencing), and ii) the nutrient content, physiology, phytohormone content, yield, and fruit quality of tomato plants. Our results indicate that soil physicochemical properties were significantly affected by the irrigation with ozonated water (OZ). We observed an increase in the content of total organic carbon (TOC), water-soluble nitrogen (WSN) and ammonium, and a decrease in soil pH due to the OZ treatment. In addition, a significant increase in alkaline phosphatase and fungal and bacterial biomass was also observed in the OZ treatment. It was observed that the prokaryotic community structure was affected by the OZ treatment, while that of fungi was undisturbed. The OZ treatment increased the photosynthetic rates of tomato plants and maintained water conditions when compared to control plants. The increased trans-Zeatin riboside (tZ-Rib) could provide rapid apical and root growth allowing adaptation to the new growing conditions. However, a more in-depth study on the physiological response of the plant to this treatment would be of interest, as it would help with the implementation of this strategy in agricultural fields in a safe manner, and with obtaining higher plant yields.

Combined pollution and soil microbial effect of pesticides and microplastics in greenhouse soil of suburban Tianjin, Northern China.

Current-used pesticides (CUPs) and plastic films are essential materials used in greenhouse cultivation, which can lead to the residual accumulation of CUPs and microplastics (MPs) over time. The impact of CUPs and MPs on soil quality and food safety cannot be overlooked. However, the combined pollution resulting from CUPs and MPs in greenhouse soil remains poorly understood. In this study, we conducted a survey at 30 greenhouse sites in the Wuqing District of Tianjin, China, to investigate the pollution levels and characteristics of CUPs and MPs using QuEChERS combined with LC-MS/MS, and density extraction, 30% H2O2 digestion and micro-fourier transform infrared spectroscopy, respectively. Additionally, we aimed to evaluate the interactions among these two pollutants, soil physicochemical properties, and the bacterial community in the soil. CUPs were frequently detected in the examined soil samples; however, they posed no significant ecological risks due to their low levels. Furthermore, MPs, which predominantly comprised fragmented and fibrous polyethylene (PE) and polypropylene (PP) particles smaller than 1.0 mm, could potentially degrade into nanoplastics, which might subsequently enter the food chain and pose a serious threat to human health. We observed no substantial correlations between CUPs and MPs, except for a negative correlation between dimethomorph and film MPs. The soil pH and total organic carbon (TOC) exhibited interactions with both types of pollutants, whereas soil clay content (CC) only correlated with CUPs, and soil available nitrogen (AN) only correlated with MPs. The variability of soil bacterial communities among the 30 sampling sites was minimal, with the dominant genus being Bacillus. Soil pH, TOC, and CC collectively exerted a strong influence on the microbial community across all samples; however, the effects of CUPs and MPs on the soil microbial structure were marginal. These results contribute to a comprehensive understanding of the environmental stress and ecological risks associated with the combined pollution of CUPs and MPs.

Long-term surface composts application enhances saline-alkali soil carbon sequestration and increases bacterial community stability and complexity.

Organic composts could remediate saline-alkali soils on agricultural land by amending soil micro-environment which is one of the main strategies for resourceful treatment and recycling of livestock manure. However, it was still unknown how long-term surface application of organic composts affects the microhabitat and bacterial community characteristics and assembly processes on the profile. We examined the features of the soil properties, bacterial community, and assembly models after 7-years composts application. Physicochemical indicators, enzyme activities, and bacterial diversity of the saline-alkali farmland were all enhanced by the surface composts application, particularly in the 0-20 cm. The network analysis showed that the surface application of composts significantly enhanced the robustness and topological characteristics of the bacterial community and that bacteria from Acidobacteriota were the keystone of the saline-alkali soils improvement. Composts also greatly increased the ecological niche of the bacterial community, while stochastic processes (mainly dispersal limitation) significantly shaped the bacterial community compared to the control. Structural equation modeling indicated that composts application promoted bacterial community succession, which in turn promoted elevated total organic carbon and improved saline-alkali soils properties. Overall, the study linked the ecological characteristics of soil microhabitats and bacterial communities during the restoration of saline-alkali soils by long-term surface application of composts, providing the management and remediation of saline-alkali agricultural soil with a theoretical foundation and technological support.

Urban greenspaces shape soil protist communities in a location-specific manner.

The impacts of urbanization on aboveground biodiversity are well studied, and its impact on soil microorganisms are also receiving increased attention. However, the impact of urbanization on the soil protists are hardly investigated. Here, we studied how urbanization and distinct urban greenspaces affect protist communities. We used amplicon sequencing of the18 S rRNA gene of samples from five types of urban greenspaces (parks, greenbelts, industrial areas, residential areas and hospital lawns), neighboring natural forests and agricultural ecosystems in Ningbo, China. We found that urban greenspaces harbored higher protist α-diversity than forests, while protist ß-diversity increased from agricultural systems to urban greenspaces to forests. Among the studied driving factors, soil bacterial α- and ß-diversity best predicted phagotrophic protist α- and ß-diversity in urban greenspaces, while differences in α- and ß-diversity of phototrophic protists were best explained by soil carbon-to-nitrogen ratio and fungal ß-diversity, respectively. Abiotic factors i.e., total phosphorus and carbon-to-nitrogen ratio, best predicted the α- and ß-diversity of protist parasites in urban greenspaces, respectively. The results revealed that the composition and drivers of protist communities vary between functional groups and urban ecosystems. Overall, our findings contribute to a better understanding of drivers of soil protist communities and indicate that soil protist communities and associated soil functions could be managed in predictable ways in urban greenspaces.

Soil properties and organochlorine compounds co-shape the microbial community structure: A case study of an obsolete site.

Organochlorine compounds (OCs) such as chlorobenzenes (CB) are persistent organic pollutants that are ubiquitous in soils at organochlorine pesticides (OCP) production sites. Long-term contamination with OCs might alter the soil microbial structure and further affect soil functions. However, the effects of OCs regarding the shaping of microbial community structures in the soils of OCs-contaminated sites remain obscure, especially in the vertical soil profile where pollutants are highly concealed. Hence this paper explored the status and causes of OCs pollution (CB, hexachlorocyclohexane (HCH), and dichlorodiphenyltrichloroethane (DDT)) in an obsolete site, and its combined effects with soil properties (pH, available phosphorus (AP), dissolved organic carbon (DOC), etc) on microbial community structure. The mean total concentration of OCs in the subsoils was up to 996 times higher than that in the topsoils, with CB constituting over 90% of OCs in the subsoil. Historical causes, anthropogenic effects, soil texture, and the nature of OCs contributed to the differences in the spatial distribution of OCs. Redundancy analysis revealed that both the soil properties and OCs were important factors in shaping microbial composition and diversity. Variation partitioning analysis further indicated that soil properties had a greater impact on microbial community structure than OCs. Significant differences in microbial composition between topsoils and subsoils were observed through linear discriminant analysis effect size (LEfSe) analysis, primarily driven by different pollutant conditions. Additionally, co-occurrence network analysis indicated that heavily contaminated subsoils exhibited closer and more intricate bacterial community interactions compared to lightly contaminated topsoils. This work reveals the impact of environmental factors in co-shaping the structure of soil microbial communities. These findings advance our understanding of the intricate interplay among organochlorine pollutants, soil properties, and microbial communities, and provides valuable insights into devising effective management strategies in OCs-contaminated soils.

Aridity drives the variability of desert soil microbiomes across north-western China.

Dryland covers >35 % of the terrestrial surface and the global extent of dryland increases due to the forecasted increase in aridity driven by climate change. Due to the climate change-driven aridity ecosystems, deserts provide one of the most hostile environments for microbial life and survival. Therefore, a detailed study was carried out to explore the deserts with different aridity levels (exposed to severe climate change) influence on microbial (bacteria, fungi, and protist) diversity patterns, assembly processes, and co-occurrence. The results revealed that the aridity (semi-arid, arid, and hyper-arid) patterns caused distinct changes in environmental heterogeneity in desert ecosystems. Similarly, microbial diversities were also reduced with increasing the aridity pattern, and it was found that environmental heterogeneity is highly involved in affecting microbial diversities under different ecological niches. Interestingly, it was found that certain microbes, including bacterial (Firmicutes), fungal (Sordariomycetes), and protistan (Ciliophora) abundance increased with increasing aridity levels, indicating that these microbes might possess the capability to tolerate the environmental stress conditions. Moreover, microbial community turnover analysis revealed that bacterial diversities followed homogenous selection, whereas fungi and protists were mostly driven by the dispersal limitation pattern. Co-occurrence network analysis showed that hyper-arid and arid conditions tightened the bacterial and fungal communities and had more positive associations compared to protistan. In conclusion, multiple lines of evidence were provided to shed light on the habitat specialization impact on microbial (bacteria, fungi, and protists) communities and composition under different desert ecosystems.

Ecological strategies of bacterial communities in prehistoric stone wall paintings across weathering gradients: A case study from the Borana zone in southern Ethiopia.

Rock art paintings represent fragile ecosystems supporting complex microbial communities tuned to the lithic substrate and climatic conditions. The composition and activity of these microbial communities associated with different weathering patterns affecting rock art sites remain unexplored. This study aimed to explore how bacterial communities adapt their ecological strategies based on substrate weathering, while also examining the role of their metabolic pathways in either biodeterioration or bioprotection of the underlying stone. SEM-EDS investigations coupled with 16S rRNA gene sequencing and PICRUSt2 analysis were applied on different weathered surfaces that affect southern Ethiopian rock paintings to investigate the relationships between the current stone microbiome and weathering patterns. The findings revealed that samples experiencing low and high weathering reached a climax stage characterized by stable microenvironments and limited resources. This condition favored K-strategist microorganisms, leading to reduced α-biodiversity and a community with a positive or neutral impact on the substrate. In contrast, moderately-weathered samples displayed diverse microhabitats, resulting in the prevalence of r-strategist bacteria, increased α-biodiversity, and the presence of specialist microorganisms. Moreover, the bacterial communities in moderately-weathered samples demonstrated the highest potential for carbon fixation, stress responses, and complete nitrogen and sulfur cycles. This bacterial community also showed the potential to negatively impact the underlying substrate. This research provided valuable insights into the little-understood ecology of bacterial communities inhabiting deteriorated surfaces, shedding light on the potential role of these microorganisms in the sustainable conservation of rock art.

Soil microbial diversity and network complexity drive the ecosystem multifunctionality of temperate grasslands under changing precipitation.

Soil microbiomes play a critical role in regulating ecosystem multifunctionality. However, whether and how soil protists and microbiome interactions affect ecosystem multifunctionality under climate change is unclear. Here, we transplanted 54 soil monoliths from three typical temperate grasslands (i.e., desert, typical, and meadow steppes) along a precipitation gradient in the Mongolian Plateau and examined their response to nighttime warming, decreased, and increased precipitation. Across the three steppes, nighttime warming only stimulated protistan diversity by 15.61 (absolute change, phylogenetic diversity) but had no effect on ecosystem multifunctionality. Decreased precipitation reduced bacterial (8.78) and fungal (22.28) diversity, but significantly enhanced soil microbiome network complexity by 1.40. Ecosystem multifunctionality was reduced by 0.23 under decreased precipitation, which could be largely attributed to the reduced soil moisture that negatively impacted bacterial and fungal communities. In contrast, increased precipitation had little impact on soil microbial communities. Overall, both bacterial and fungal diversity and network complexity play a fundamental role in maintaining ecosystem multifunctionality in response to drought stress. Protists alter ecosystem multifunctionality by indirectly affecting microbial network complexity. Therefore, not only microbial diversity but also their interactions (regulated by soil protists) should be considered in evaluating the responses of ecosystem multifunctionality, which has important implications for predicting changes in ecosystem functioning under future climate change scenarios.

Plastic residues alter soil microbial community compositions and metabolite profiles under realistic conditions.

Wide usage of plastic coupled with mismanagement has created a humongous environmental hazard threatening entire ecosystems. To date, the potential effects of plastic debris-induced soil nutrition substance changes and the relevant microbial metabolic behavior remain unclear. Here, we studied the effect of plastic films polyethylene and polylactic acid in differential soil environments (farmland, woodland, and wetland) for 120 days. Soil enzyme activities (urease, neutral phosphatase, and catalase) and nutrition substance (NH4+-N, available P, available K, and soil organic matter) present obvious variations in polylactic acid groups compared to polyethylene-treated samples. 16S rRNA gene sequencing indicates that several bacteria abundance such as Bacteroidales, Actinobacteriota, Nitrososphaeraceae, Pyrinomonadalcs, Muribaculaceae, exhibited obvious up-regulation or down-regulation, and simultaneously, the carbon, nitrogen, and phosphorus cycling relevant species Bryobacter, Bradyrhizobium, and Sphingomonas, expressed wider margin of down-regulation in abundance in plastic treatment soil samples. As a result, the abundance of metabolites including sugar, amino acid, and fatty acids, which may associated with nutrition substance metabolic pathways, were significantly altered in the stress of plastic. These findings provide valuable information on the environmental effects of plastics, and the relationships of subsequent nutrition substance changes and microbial metabolic behavior.

Arbuscular mycorrhizal fungi alleviate erosional soil nitrogen loss by regulating nitrogen cycling genes and enzymes in experimental agro-ecosystems.

Nutrient losses from agricultural ecosystems are increasingly threatening global environmental and human health. Although arbuscular mycorrhizal (AM) fungi have the potential to regulate soil nitrogen (N) loss by enhancing plant uptake and soil particle immobilization, the microbial mechanism behind such mycorrhizal effect is unknown. Herein, by conducting a simulated erosion experiment, we compared the effects of exogenous AM fungal inoculation (Funneliformis mosseae) on the gene abundances and enzyme activities of N-cycling processes, and associated such effect to N uptake and loss. The experiment was composed of combinations of two AM fungal treatments (control vs. AM fungal inoculation), two crops (maize vs. soybean) and two slopes of the plots (6° vs. 20°). The experimental plots subjected to natural rainfalls to simulate the erosion events. We showed that the effects of AM fungi were greater in the maize soils than in the soybean soils. In the maize soils, AM fungi increased the abundances of N-fixing (+81.1 %) and nitrifying genes (+200.7 %) and N cycling enzyme activity (+22.3 %). In the soybean soils, AM fungi increased the N-fixing gene abundance (+36.9 %) but decreased the abundance of nitrifying genes (-18.9 %). The abundance of N-fixing gene was positively correlated with N uptake but negatively correlated with N loss. Additionally, AM fungi enhanced the effects of mycorrhizal colonization and moisture but decreased the effects of nutrients on soil microbial metrics related to N-cycling processes. Therefore, AM fungal inoculation enhanced N uptake and reduced N loss by increasing N-fixing gene abundance, and that AM fungi should be preferably used for the low N environments or for the ecosystems highly limited by or competing for N.

Land abandonment transforms soil microbiome stability and functional profiles in apple orchards of the Chinese Losses Plateau.

Land abandonment is considered an effective strategy for ecological restoration on a global scale. However, few studies have focused on how environmental heterogeneity associated with the age of land abandonment affects the assembly and potential functions of the soil microbial community. In the present study, we investigated the community assembly of soil bacteria and fungi as well as the stability of soil networks and their potential functions in the chronosequence of abandoned apple orchards. We elucidated that the Shannon diversity of bacteria and the richness of fungi increased as land abandonment progressed. In addition, land abandonment destabilized the microbial network stability but increased network complexity. Soil available nitrogen, total carbon, and moisture are the potentially important factors in shaping the soil microbial assembly. Importantly, we showed that the microbial community diversity and functional diversity presented a synchronization effect in response to the different stages of land abandonment. Furthermore, specific bacterial taxa related to carbon fixation, dissimilatory nitrate reduction, and organic phosphorus mineralization were significantly enriched during the early abandonment stage. Collectively, these results indicate that land abandonment significantly transformed soil microbiome assembly and functional adaptation during the restoration process. These findings provide valuable insights into the influence of ecological restoration on soil microbiome and ecosystem functions in arable areas.

Effect of Streptomyces JD211 application on soil physicochemical properties and N2O emission characteristics of rice rhizosphere.

Biocontrol agent, as a pollution-free and sustainable plant disease control method, can inhibit the spread of soil-borne diseases and promote the growth of crops. However, there are few studies on the effect of biocontrol agent on N2O emission in rice soil. In this study, after the application of the biocontrol agent Streptomyces JD211, N2O emission from rice soil were measured, and the relationship between the agent and soil N2O emissions were studied in soil chemistry and molecular biology. The results showed that the application of Streptomyces JD211 can significantly reduce the rate of N2O emission from rice soil. The NH4+-N and NO3--N contents in rice soil decreased in a short period of time after the application of Streptomyces JD211, while the mineral N content in the soil remained stable with rice growth. 16S rRNA gene sequencing and metagenomic sequencing revealed Streptomyces JD211 application mainly increased the relative abundance of Burkholderia and Streptomyces in the soil microbial community, reduced the relative abundance of hao, norB, norC genes, and increased the relative abundance of nosZ and hcp genes. Streptomyces JD211 application promoted N2O transformation and weakened N2O production pathways, which ultimately reduced N2O emissions from rice soils. This study provided new insight of biocontrol agents to regulate soil N2O emissions, which is of great significance for the development and application of biocontrol bacteria and farmland environmental protection.

Metagenomics-assembled analysis revealed the characteristics of antibiotic resistome and community coalescence in the soils irrigated with different irrigation materials.

Antibiotic resistance has received widespread attention in recent years. Soil irrigation and fertilization are routine agricultural practices, but also lead to the spread of antibiotic resistance genes (ARGs) in soil-crop system such as via resistome coalescence. Despite community coalescence being ubiquitous and important in natural ecosystems, little research has been done to investigate resistome coalescence during soil irrigation activities. In this study, the characteristics of antibiotic resistome and community coalescence in the soils irrigated with different irrigation materials (wastewater, wastewater-river water, and wastewater-manure) have been revealed by utilizing microcosm experiments and high-throughput sequencing-based metagenomic assembly approaches. Results showed irrigation and coalescence changed soil quality and resistome. Totally, 789 unique ARGs were identified in the irrigation system, including some emerging ARGs. The abundance and diversity of ARGs increased in the coalesced soils, mainly due to the newly imported ARGs from irrigation materials. Relatively, the soils irrigated with wastewater and manure showed higher level of ARGs. Irrigation with the mixtures containing river water caused greater loss of indigenous taxa, while the community structure of mixing treatment with manure changed more dramatically. Interestingly, the succession of community in coalesced soils was influenced by transient competition for resources and ecological niche width, and the highest abundance and diversity of microorganisms and ARGs were found in the initial phase of coalescence, followed by a gradual succession towards the original community. With increasement of wastewater in the irrigation materials, the soil community showed a stepwise change rather than linear change. Notably, natural deposit of irrigation materials reduced their impacts on the ARGs in the coalesced soils. Findings provide new insights into the resistome coalescence during agricultural practices for reducing the spread risks of ARGs.

Nitrogen deposition raises temperature sensitivity of soil organic matter decomposition in subtropical forest.

Subtropical ecosystems are strongly affected by nitrogen (N) deposition, impacting soil organic matter (SOM) availability and stocks. Here we aimed to reveal the effects of N deposition on i) the structure and functioning of microbial communities and ii) the temperature sensitivity (Q10) of SOM decomposition. Phosphorus (P) limited evergreen forest in Guangdong Province, southeastern China, was selected, and N deposition (factor level: N (100 kg N ha-1 y-1 (NH4NO3)) and control (water), arranged into randomized complete block design (n = 3)) was performed during 2.5 y. After that soils from 0 to 20 cm were collected, analyzed for the set of parameters and incubated at 15, and 25, and 35 °C for 112 days. N deposition increased the microbial biomass N and the content of fungal and Gram-positive bacterial biomarkers; activities of beta-glucosidase (BG) and acid phosphatase (ACP) also increased showing the intensification of SOM decomposition. The Q10 of SOM decomposition under N deposition was 1.66 and increased by 1.4 times than under control. Xylosidase (BX), BG, and ACP activities increased with temperature under N but decreased with the incubation duration, indicating either low production and/or decomposition of enzymes. Activities of polyphenol-(PPO) and peroxidases (POD) were higher under N than in the control soil and were constant during the incubation showing the intensification of recalcitrant SOM decomposition. At the early incubation stage (10 days), the increase of Q10 of CO2 efflux was explained by the activities of BX, BQ, ACP, and POD and the quality of the available dissolved organic matter pool. At the later incubation stages (112 days), the drop of Q10 of CO2 efflux was due to the depletion of the labile organic substances and the shift of microbial community structure to K-strategists. Thus, N deposition decoupled the effects of extracellular enzyme activities from microbial community structure on Q10 of SOM decomposition in the subtropical forest soil.

Metagenomic insights into the microbial cooperative networks of a benz(a)anthracene-7,12-dione degrading community from a creosote-contaminated soil.

Genotoxicity of PAH-contaminated soils can eventually increase after bioremediation due to the formation and accumulation of polar transformation products, mainly oxygenated PAHs (oxy-PAHs). Biodegradation of oxy-PAHs has been described in soils, but information on the microorganisms and mechanisms involved is still scarce. Benz(a)anthracene-7,12-dione (BaAQ), a transformation product from benz(a)anthracene frequently detected in soils, presents higher genotoxic potential than its parent PAH. Here, using sand-in-liquid microcosms we identified a specialized BaAQ-degrading subpopulation in a PAH-contaminated soil. A BaAQ-degrading microbial consortium was obtained by enrichment in sand-in-liquid cultures with BaAQ as sole carbon source, and its metagenomic analysis identified members of Sphingobium, Stenotrophomonas, Pusillimonas, Olivibacter, Pseudomonas, Achromobacter, and Hyphomicrobiales as major components. The integration of data from metabolomic and metagenomic functional gene analyses of the consortium revealed that the BaAQ metabolic pathway was initiated by Baeyer-Villiger monooxygenases (BVMOs). The presence of plasmid pANTQ-1 in the metagenomic sequences, identified in a previous multi-omic characterization of a 9,10-anthraquinone-degrading isolate recovered from the same soil, suggested the occurrence of a horizontal gene transfer event. Further metagenomic analysis of the BaAQ-degrading consortium also provided insights into the potential roles and interactions within the consortium members. Several potential auxotrophies were detected, indicating that relevant nutritional interdependencies and syntrophic associations were taking place within the community members, not only to provide suitable carbon and energy sources, but also to supply essential nutrients and cofactors. Our work confirms the essential role that BVMO may play as a detoxification mechanism to mitigate the risk posed by oxy-PAH formation during bioremediation of contaminated soils.

Effects of fir-wood biochar on CH4 oxidation rates and methanotrophs in landfill cover soils packed at three different proctor compaction levels.

Application of biochar to landfill cover soils can purportedly improve methane (CH4) oxidation rates, but understanding the combined effects of soil texture, compaction, and biochar on the activity and composition of the methanotrophs is limited. The amendment of wood biochar on two differently textured landfill cover soils at three compaction levels of the Proctor density was explored by analyzing changes in soil physical properties relevant to methane oxidation, the effects on CH4 oxidation rates, and the composition of the methanotrophic community. Loose soils with and without biochar were pre-incubated to equally elevate the CH4 oxidation rates. Hereafter, soils were compacted and re-incubated. Methane oxidation rates, gas diffusivity, water retention characteristics, and pore size distribution were analyzed on the compacted soils. The relative abundance of methanotrophic bacteria (MOB) was determined at the end of both the pre-incubation and incubation tests of the packed samples. Biochar significantly increased porosity at all compaction levels, enhancing diffusion coefficients. Also, a re-distribution in pore sizes was observed. Increased gas diffusivity from low compaction and amendment of biochar, though, did not reflect higher methane oxidation rates due to high diffusive oxygen fluxes over the limited height of the compacted soil specimens. All soils, with and without biochar, were strongly dominated by Type II methanotrophs. In the sandy soil, biochar amendment strongly increased MOB abundance, which could be attributed to a corresponding increase in the relative abundance of Methylocystis species, while no such response was observed in the clayey soil. Compaction did not change the community composition in either soil. Fir-wood biochar addition to landfill cover soils may not always enhance methanotrophic activity and hence reduce fugitive methane emissions, with the effect being soil-specific. However, especially in finer and more compacted soils, biochar amendment can maintain soil diffusivity above a critical level, preventing the collapse of methanotrophy.

Reevaluation and novel insights into amino sugar and neutral sugar necromass biomarkers in archaea, bacteria, fungi, and plants.

Soil microbial necromass is an important contributor to soil organic matter (>50%) and it is largely composed of microbial residues. In soils, fragmented cell wall residues are mostly found in their polysaccharide forms of fungal chitin and bacterial peptidoglycan. Microbial necromass biomarkers, particularly amino sugars (AS) such as glucosamine (GlcN) and muramic acid (MurA) have been used to trace fungal and bacterial residues in soils, and to distinguish carbon (C) found in microbial residues from non-microbial organic C. Neutral sugars (NS), particularly the hexose/pentose ratio, have also been proposed as tracers of plant polysaccharides in soils. In our study, we extended the range of biomarkers to include AS and NS compounds in the biomass of 120 species belonging to archaea, bacteria, fungi, or plants. GlcN was the most common AS found in all taxa, contributing 42-91% to total AS content, while glucose was the most common NS found, contributing 56-79% to total NS. We identified talosaminuronic acid, found in archaeal pseudopeptidoglycan, as a new potential biomarker specific for Euryarchaeota. We compared the variability of these compounds between the different taxonomic groups using multivariate approaches, such as non-metric multidimensional scaling (NMDS) and partial least squares discriminant analysis (PLS-DA) and statistically evaluated their biomarker potential via indicator species analysis. Both NMDS and PLS-DA showcased the variability in the AS and NS contents between the different taxonomic groups, highlighting their potential as necromass residue biomarkers and allowing their extension from separating bacterial and fungal necromass to separating microbes from plants. Finally, we estimated new conversion factors where fungal GlcN is converted to fungal C by multiplying by 10 and MurA is converted to bacterial C by multiplying by 54. Conversion factors for talosaminuronic acid and galactosamine are also proposed to allow estimation of archaeal or all-microbial necromass residue C, respectively.

Climate warming enhances microbial network complexity by increasing bacterial diversity and fungal interaction strength in litter decomposition.

Microbial communities are important drivers of plant litter decomposition; however, the mechanisms of microbial co-occurrence networks and their network interaction dynamics in response to climate warming in wetlands remain unclear. Here, we conducted a 1.5-year warming experiment on the bacterial and fungal communities involved in litter decomposition in a typical wetland. The results showed that warming accelerated the decomposition of litter and had a greater effect on the diversity of bacteria than on that of fungi. Dominant bacterial communities, such as Bacteroidia, Alphaproteobacteria, and Actinobacteria, and dominant fungal communities, such as Leotiomycetes and Sordariomycetes, showed significant positive correlations with lignin and cellulose. Co-occurrence networks revealed that the average path length and betweenness centrality under warming conditions increased in the bacterial community but decreased in the fungal community. Both bacterial and fungal networks in the 2.0 °C warming treatment had the highest ratio of positive links (58.53 % and 98.14 %), indicating that moderate warming can promote the positive correlations and symbiotic relationships observed in the microbial community. This also suggests that small-world characteristics and weak-link advantages accelerate diffusion, and scale-free features facilitate propagation in microbial communities in response to climate warming. Logistic growth and Lotka-Volterra competition models revealed that climate warming enhances microbial network complexity mainly by increasing bacterial diversity and fungal interaction strength in litter decomposition. However, the symbiotic relationship decreased slightly under 4.0 °C warming, indicating that climate warming is a random attack rather than a targeted attack, and the microbial network has strong resistance to random attack, as shown by the highly robust dynamic performance of the microbial network in litter decomposition. Overall, the microbial community in litter decomposition responded to climate warming and shifted its network interactions, leading to further changes in emergent network topology and dynamics, thus accelerating litter decomposition in wetlands.