Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 147
Filtrar
1.
Mycoses ; 63(1): 21-29, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31610041

RESUMO

BACKGROUND: Despite the worldwide prevalence of dermatophyte infections, only a few genes are reported to be related to dermatophyte infections. In addition, the mechanism by which different ecological dermatophytes infection leads to varying intensity of inflammation remains unclear. OBJECTIVES: To investigate the mechanism of varying intensity of skin inflammation caused by different ecological dermatophytes infection. METHODS: We infected HaCaT cells with anthropophilic and geophilic dermatophytes to mimic various ecological dermatophyte infections. RNA-sequencing (RNA-seq) was employed to identify the change in the gene expression of HaCaT cells. To verify the expression of differentially expressed genes (DEGs), we selected 18 HaCaT cells genes to conduct qPCR experiments. In addition, immunoblotting was conducted to validate key genes from the MAPK signalling pathway. RESULTS: After HaCaT cells were infected with the anthropophilic Trichophyton rubrum (T rubrum) and the geophilic Microsporum gypseum (M gypseum), 118 and 619 differentially expressed genes were identified in HaCaT cells, respectively. These genes may provide a clue as to how keratinocytes respond to anthropophilic and geophilic dermatophytes. We also found that JUN may play a critical role in keratinocytes infected with M gypseum. CONCLUSIONS: Differential gene expression in HaCaT cells may account for the various clinical presentation caused by anthropophilic and geophilic dermatophytes infections. In addition, the intense inflammatory reaction of M gypseum infection may be triggered by activating the JNK-JUN signalling pathway.


Assuntos
Arthrodermataceae , Interações Hospedeiro-Patógeno/imunologia , Queratinócitos/metabolismo , Queratinócitos/microbiologia , Arthrodermataceae/patogenicidade , Linhagem Celular , Dermatomicoses/genética , Dermatomicoses/imunologia , Dermatomicoses/microbiologia , Perfilação da Expressão Gênica , Humanos , Queratinócitos/imunologia , Microsporum/patogenicidade , Transdução de Sinais/genética , Trichophyton/patogenicidade
2.
Int J Mol Med ; 44(6): 2047-2056, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31573050

RESUMO

Microsporum canis (M. canis) is a common pathogen that causes tinea capitis and is present worldwide. The incidence of M. canis infection, particularly tinea capitis, has been increasing in China. In our previous studies, family of serine hydrolases 1 (FSH1) was identified as a potential virulence factor in tinea capitis infection caused by M. canis. To determine the function of this gene in M. canis, FSH1 was knocked down using double­stranded RNA interference mediated by Agrobacterium tumefaciens. Reverse transcription­quantitative PCR analysis was used to confirm gene knockdown. Loss of FSH1 expression by RNAi resulted in a minor phenotype alteration, but M. canis pathogenicity in guinea pig cutaneous infection was decreased compared with the wild­type strain. To the best of our knowledge, the present study is the first to demonstrate that FSH1 is associated with macroconidia septa formation and is an important contributor to M. canis virulence. These findings may advance the understanding of the function of the FSH1 gene and provide a foundation for future studies on macroconidia septa formation and pathogenicity of M. canis.


Assuntos
Dermatomicoses/microbiologia , Hidrolases/genética , Microsporum/genética , Tinha do Couro Cabeludo/genética , Arthrodermataceae/genética , Arthrodermataceae/patogenicidade , China , Dermatomicoses/genética , Dermatomicoses/patologia , Técnicas de Silenciamento de Genes , Humanos , Microsporum/patogenicidade , Fenótipo , Tinha do Couro Cabeludo/microbiologia , Tinha do Couro Cabeludo/patologia , Virulência/genética
3.
Artigo em Inglês, Alemão | MEDLINE | ID: mdl-31434126

RESUMO

Dermatophytosis is a cutaneous infection, caused by several types of keratophilic fungi (dermatophytes). It represents a serious and common contagious skin disease in dogs and cats. The significance of this disease for pet owners is based on the zoonotic potential. The prevalence varies with climate and local dermatophyte infestation. The most common infection in dogs and cats are caused by the genera Microsporum (M.), Nannizzia (N.) or Trichophyton (T.). The aim of this article is to summarise novel taxonomy, diagnosis, and treatment recommendations as well as the recently revised recommendations of the World Association of Veterinary Dermatology.


Assuntos
Doenças do Gato/microbiologia , Doenças do Cão/microbiologia , Tinha/veterinária , Animais , Arthrodermataceae/genética , Arthrodermataceae/isolamento & purificação , Arthrodermataceae/patogenicidade , Biópsia/veterinária , Doenças do Gato/diagnóstico , Doenças do Gato/terapia , Gatos , Diagnóstico Diferencial , Doenças do Cão/diagnóstico , Doenças do Cão/terapia , Cães , Meio Ambiente , Humanos , Microsporum/isolamento & purificação , Microsporum/patogenicidade , Tinha/diagnóstico , Tinha/microbiologia , Tinha/terapia , Trichophyton/patogenicidade , Zoonoses/diagnóstico , Zoonoses/microbiologia
4.
Med Mycol J ; 60(2): 45-49, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31155571

RESUMO

Lophophyton gallinae (Microsporum gallinae) is a zoophilic fungus that causes ringworm in chickens and related species, and occasionally in humans. There are 45 human cases worldwide including a Japanese case from Okinawa in 2009. After the finding of the human L. gallinae case, 793 chickens in Japan, including 293 from the mainland and 500 from the Nansei Island areas, were investigated to determine the prevalence of dermatophytes and their related fungal species. The survey was carried out from December 2008 to March 2013. Various dermatophytes and related fungal species were isolated from the studied chickens, with a prevalence of 24.6%. In total, 224 dermatophytes and related species were isolated in the survey. The most commonly isolated species included, in descending order of frequency, Arthroderma multifidum, Aphanoascus terreus, and Chrysosporium spp. Ar. multifidum and Ap. terreus have no record of pathogenicity, and the present isolates of Chrysosporium spp. were not matched to pathogenic Chrysosporium spp. based on the ITS rDNA sequences. Interestingly, an L. gallinae isolate was detected in a male 10-month-old shamo (fighting cock) from the main island. Furthermore, one strain of Arthroderma simii was also isolated as the second record in Japan following that from an imported chimpanzee. Although L. gallinae and Ar. simii are likely to be endemic in our country, the transmission of dermatophytosis from chickens to humans is unlikely to occur because of the reduced chances for citizens to come in contact with chickens due to various factors.


Assuntos
Arthrodermataceae/isolamento & purificação , Arthrodermataceae/patogenicidade , Galinhas/microbiologia , Chrysosporium/isolamento & purificação , Chrysosporium/patogenicidade , Dermatomicoses/microbiologia , Dermatomicoses/veterinária , Microsporum/isolamento & purificação , Microsporum/patogenicidade , Zoonoses/microbiologia , Animais , Arthrodermataceae/genética , Chrysosporium/genética , DNA Fúngico/genética , DNA Ribossômico/genética , Dermatomicoses/transmissão , Humanos , Japão , Microsporum/genética , Pan troglodytes/microbiologia , Análise de Sequência de DNA
5.
J Invest Dermatol ; 138(8): 1744-1753, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29571944

RESUMO

Despite worldwide prevalence of superficial mycoses, the immune response in dermatophytosis has scarcely been investigated. In this study, we developed a model of superficial skin infection in C57BL/6 mice with Microsporum canis, a highly prevalent human pathogen. This model mimics mild inflammatory human dermatophytosis, characterized by neutrophil recruitment and fungal invasion limited to the epidermis and exhibits the establishment of a specific T helper type 17 immune response during infection. By using IL-17RA- or IL-17A/F-deficient mice we showed that, in the absence of a functional IL-17 pathway, M. canis extensively colonizes the epidermis and promotes an exaggerated skin inflammation and a shift to an IFN-γ-mediated (T helper type 1) response. IL-17 signaling was not involved in neutrophil influx to skin or fungal invasion to deeper tissues. Finally, this study shows that skin langerin-expressing cells contribute to the antifungal T helper type 17 response in vivo. In conclusion, these data directly show a dual function of IL-17 cytokines in dermatophytosis by controlling superficial infection and down-modulating a T helper type 1 antifungal response.


Assuntos
Interações Hospedeiro-Patógeno/imunologia , Microsporum/imunologia , Transdução de Sinais/imunologia , Células Th17/imunologia , Tinha/imunologia , Animais , Modelos Animais de Doenças , Epiderme/imunologia , Epiderme/microbiologia , Epiderme/patologia , Humanos , Interleucina-17/genética , Interleucina-17/imunologia , Interleucina-17/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microsporum/patogenicidade , Infiltração de Neutrófilos/imunologia , Receptores de Interleucina-17/genética , Receptores de Interleucina-17/imunologia , Receptores de Interleucina-17/metabolismo , Células Th17/metabolismo , Tinha/microbiologia , Tinha/patologia
6.
Acta Dermatovenerol Croat ; 25(2): 151-154, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28871931

RESUMO

Microsporum (M.) canis is the most common fungus to cause tinea capitis in Europe, especially in the Mediterranean region and South and Central Europe. Fungal scalp infections caused by M. canis tend to be non-inflammatory. Recently, a growing number of cases of tinea capitis characterized by inflammatory infection caused by M. canis and M. gypseum have been registered. We present a case of highly inflammatory tinea capitis, also known as kerion celsi, caused by M. canis in a 6-year-old-patient. Scalp infections due to M. canis are a growing problem in dermatological practice. Changes in epidemiology, etiology, and clinical patterns of fungal infections due to M. canis are significant. Greater awareness of this problem is needed in order to establish proper diagnosis and successful treatment strategy for these patients.


Assuntos
Microsporum/isolamento & purificação , Tinha do Couro Cabeludo/microbiologia , Tinha do Couro Cabeludo/patologia , Criança , Feminino , Humanos , Microsporum/patogenicidade
7.
Med Mycol J ; 58(2): J29-J33, 2017.
Artigo em Japonês | MEDLINE | ID: mdl-28566664

RESUMO

Several pathogenic fungi and cases related to Japanese medical mycologists were reviewed. Trichosporon inkin (as Sarcinomyces inkin) was reported as a pathogen of scrotal lesion by Oho in 1921, and Trichosporon asahii was isolated from generalized keratotic lesions in 1922 by Akagi in Japan. They were once included in Trichophyton beigelii, but then based on revision using DNA molecular technology, were returned to their original names.Microsporum ferrugineum was reported by Ota as a causative dermatophyte of tinea capitis in Japan and surrounding areas. It was once classified under the genus Trichophyton, but after the discovery of characteristic rough-walled macroconidia belonging to genus Microsporum, the fungus was again assigned to the original name.


Assuntos
Micologia/história , Trichosporon , DNA Fúngico , História do Século XX , História do Século XXI , Humanos , Japão , Microsporum/classificação , Microsporum/patogenicidade , Micologia/tendências , Pele/microbiologia , Pele/patologia , Tinha do Couro Cabeludo/microbiologia , Tinha do Couro Cabeludo/patologia , Trichophyton , Trichosporon/genética , Trichosporon/isolamento & purificação , Trichosporon/patogenicidade
8.
Mycoses ; 60(6): 387-393, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28198097

RESUMO

In humans worldwide, Microsporum sp. is a frequent agent of dermatophytsosis. When considering the emergence of resistant fungi and the clinical relevance of dermatophytosis, terpene antifungal activity is of great interest. Linalool is a monoterpene alcohol with pharmacological properties. In this study, antifungal in vitro activity of linalool and ketoconazole (as a positive control) were evaluated against clinical isolates of M. canis and M. gypseum. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of each drug were determined by broth microdilution. The effects of the drugs (1/2MIC, MIC, 2xMIC) on radial mycelial growth, conidial production and germination were analysed. The effect on the fungal cell membrane (release of intracellular material) was also investigated. Linalool (MIC: 128 µg/mL) and ketoconazole (MIC: 64 µg/mL) were effective in inhibiting all dermatophytes studied. The MFC values of linalool ranged between 128 and 256 µg/mL, whereas ketoconazole showed MFC values of from 64 to 256 µg/mL. Linalool (at MIC and 2xMIC) and ketoconazole (at 1/2MIC, MIC, 2xMIC) inhibited mycelial growth (P < 0.05). The drugs (1/2MIC, MIC, 2xMIC) were also active on conidiogenesis and conidia germination, causing complete inhibition (P < 0.05). Linalool caused leakage of intracellular material. Our study supports the use of linalool as a potential antifungal agent against M. canis and M. gypseum.


Assuntos
Antifúngicos/farmacologia , Microsporum/efeitos dos fármacos , Monoterpenos/farmacologia , Monoterpenos Acíclicos , Cetoconazol/farmacologia , Testes de Sensibilidade Microbiana , Microsporum/patogenicidade
9.
J Feline Med Surg ; 19(4): 435-439, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26927817

RESUMO

Objectives The objective of this study was to evaluate the efficacy of vacuuming and three carpet cleaning methods for the removal of Microsporum canis spores and hairs from experimentally contaminated carpets. Methods Sterile Berber carpeting was artificially contaminated with naturally infective M canis hairs and spores. Carpet swatches were vacuumed for 10 s, 30 s and 60 s, and then cultured. Three carpet cleaning methods were evaluated on area rugs experimentally contaminated with infective material: a beater brush carpet shampooing, beater brush carpet shampooing post-disinfectant application and hot water extraction. Home cleaning products labeled as having efficacy against Trichophyton species were used in addition to 1% potassium peroxymonosulfate. Carpets were cultured at 24 h, 48 h and 7 days after cleaning. Good efficacy was no detectable spores at post-cleaning culture. Results All pretreatment carpet samples were culture positive for M canis (>300 colony-forming units [cfu]/site). Vacuuming did not decontaminate carpets but did remove intact hairs. Spores were not detected by wipe samples after two washings with an upright beater brush carpet shampooer or pretreatment with a disinfectant prior to carpet shampooing. Carpets cleaned with one hot water extraction technique had a decrease from 300 cfu/site to a mean of 5.5 cfu/site at 24 and 48 h post-cleaning and 2 cfu/site at day 7. The use of disinfectants was associated with odor, even when dry, and permanent discoloration. Hot water extraction cleaning was associated with the fastest drying time and no discoloration. Conclusions and relevance Carpets exposed to M canis can be disinfected via carpet shampooing or hot water extraction cleaning. Vacuuming of carpets is recommended to remove infective hairs. For homes, exposed carpeting can be decontaminated by routine washing with a carpet shampooer (twice) or hot water extraction. Use of pretreatment with a disinfectant is recommended when a high level of overall decontamination is needed in an animal facility with necessary carpeted surfaces (eg, entryway carpet mats).


Assuntos
Doenças do Gato/prevenção & controle , Pisos e Cobertura de Pisos , Microsporum/patogenicidade , Esporos Fúngicos/patogenicidade , Animais , Gatos , Descontaminação , Desinfetantes , Cabelo/microbiologia
10.
Mycopathologia ; 182(1-2): 215-227, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27590362

RESUMO

The genera Trichophyton, Microsporum, and Epidermophyton include filamentous fungi that cause dermatophytosis, a superficial infection of the skin, stratum corneum, nail beds, and hair follicles. The ability of dermatophytes to adhere to these substrates and adapt to the host environment is essential for the establishment of infection. Several fungal enzymes and proteins participate in this adaptive response to the environment and to keratin degradation. Transcription factors such as PacC and Hfs1, as well as heat shock proteins, are involved in sensing and adapting to the acidic pH of the skin in the early stages of fungal-host interaction. During dermatophyte growth, with keratin as the sole carbon source, the extracellular pH shifts from acidic to alkaline. This creates an environment in which most of the known keratinolytic proteases exhibit optimal activity. These events culminate in the establishment and maintenance of the infection, which can be chronic or acute depending on the dermatophyte species. This review focuses on these and other molecular aspects of the dermatophyte-host interaction.


Assuntos
Epidermophyton/patogenicidade , Interações Hospedeiro-Patógeno , Microsporum/patogenicidade , Tinha/microbiologia , Tinha/patologia , Trichophyton/patogenicidade , Animais , Epidermophyton/enzimologia , Humanos , Hidrólise , Queratinas/metabolismo , Microsporum/enzimologia , Trichophyton/enzimologia
12.
Mycoses ; 58(1): 40-7, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25476038

RESUMO

Dermatophytes are some of the most common fungal pathogens in both humans and animals. These fungi release enzymes (e.g., keratinases) that play roles in their pathogenesis. Little is known about their haemolytic and co-haemolytic (CAMP-like) activities; however, in bacteria, these components play significant roles in pathogenesis. This study characterised these two factors in 45 dermatophyte strains (representing the genera Arthroderma, Epidermophyton, Microsporum and Trichophyton) using Columbia agar (CA) supplemented with 5% bovine, ovine and equine erythrocytes. Haemolysis was best observed on CA supplemented with ovine erythrocytes followed by equine and bovine erythrocytes, while CAMP-like reactions occurred using bovine and ovine but not equine erythrocytes. Haemolytic and CAMP-like activities were best observed using ovine and bovine erythrocytes in CA in 44 and 38 strains at 7 and 3 days respectively. Most dermatophytes recovered from both symptomatic and asymptomatic lesions had haemolytic and CAMP-like activities. We suggest that the haemolytic and CAMP-like activities are not correlated with ecological characteristics, isolation sites or clinical manifestations of dermatophytic fungi. We also believe that this study has the potential to contribute to the existing literature on dermatophytes and dermatophyte pathogenesis.


Assuntos
Arthrodermataceae/metabolismo , Arthrodermataceae/patogenicidade , Proteínas Fúngicas/metabolismo , Proteínas Hemolisinas/metabolismo , Hemólise , Animais , Arthrodermataceae/isolamento & purificação , Bovinos , Epidermophyton/isolamento & purificação , Epidermophyton/metabolismo , Epidermophyton/patogenicidade , Cavalos , Humanos , Microsporum/isolamento & purificação , Microsporum/metabolismo , Microsporum/patogenicidade , Ovinos , Trichophyton/isolamento & purificação , Trichophyton/metabolismo , Trichophyton/patogenicidade
14.
Med Mycol J ; 55(2): J79-83, 2014.
Artigo em Japonês | MEDLINE | ID: mdl-24943212

RESUMO

We report two cases of dermatophytosis caused by Microsporum (M.) gypseum. One case was a 59-year-old healthy woman who complained of itchy annular erythema on her right forearm. We isolated M. gypseum from scales on the forearm. The other case was a 73-year-old midwife who had developed infiltrated erythema on her face for 6 months. M. gypseum was isolated from scales of the nose. Both women liked gardening and M. gypseum was isolated from the garden soil of these women by a hair-baiting technique. The first case had a cat, a mouse and an owl, and the second had a dog. Hairbrush culture of these pets, however, was negative. So we concluded both cases were infected with M. gypseum from garden soil. We isolated M. gypseum from soil collected in Chigasaki city. Of the 7 fungal cultures from 10 samples, 2 cultures were identified as M. gypseum.


Assuntos
Microsporum/isolamento & purificação , Microsporum/patogenicidade , Microbiologia do Solo , Tinha/microbiologia , Idoso , Feminino , Humanos , Japão , Pessoa de Meia-Idade , Micologia/métodos , Pele/microbiologia , Pele/patologia , Tinha/patologia
15.
Georgian Med News ; (224): 26-30, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24323960

RESUMO

The aim of the study was to reveal peculiarities of the clinical symptoms and forms of Tinea capitis caused by etiological agents that are common in our country. The study was conducted on 176 ambulatory patients who approached the National Centre in 2009-2013. Inclusion criterion was simultaneous presence of clinical symptoms and positive result of microscopic study. For cultural examination was used Sabouraud's dextrose agar with the antibiotic chloramphenicol. Clinical manifestations were divided into inflammatory and non-inflammatory (i.e. slightly manifested inflammatory signs) lesions. 85(48,3%) inflammatory and 91(51,7%) non-inflammatory cases of Tinea capitis were revealed. Clinical forms were distributed in following way: kerion 73 (41,5%), grey patch with single lesions 71 (40,3%), seborrheic dermatitis-like form 14 (8%), agminate folliculites 12 (6,8%) and black-dot dermatophytosis 6 (3,4%). In 41(89,1%) of the cases etiological agent of the kerion was Trichophyton mentagrophytes; in 41(85,4%) of the cases etiological agent for the grey patch with single lesions was Microsporum canis. Important clinical and etiological relationship was revealed between kerion and Trichophyton mentagrophytes, as well as between grey patch with single lesions and Microsporum canis. In case of inflammatory forms (predominantly kerion) caused by Trichophyton verrucosum and Trichophyton mentagrophytes ID reaction was manifested by disseminated follicular papules.


Assuntos
Dermatomicoses/patologia , Inflamação/patologia , Tinha do Couro Cabeludo/patologia , Tinha do Couro Cabeludo/parasitologia , Adolescente , Criança , Pré-Escolar , Dermatomicoses/etiologia , Dermatomicoses/parasitologia , Feminino , República da Geórgia , Humanos , Lactente , Inflamação/etiologia , Masculino , Microsporum/patogenicidade , Tinha do Couro Cabeludo/etiologia , Trichophyton/patogenicidade
16.
Rev. iberoam. micol ; 29(4): 238-240, oct.-dic. 2012.
Artigo em Espanhol | IBECS | ID: ibc-105668

RESUMO

Antecedentes. El gato, considerado el principal reservorio de Microsporum canis, cumple un rol importante en la aparición de las dermatomicosis cuando es habitante de zonas urbanas. Objetivos. Conocer y analizar la frecuencia de aislamiento de dermatofitos zoonóticos en una muestra de felinos del área urbana del Gran Mendoza. Métodos. Se seleccionaron animales de compañía y menores de un año que estuvieran transitoriamente en refugios y criaderos de zonas urbanas del Gran Mendoza. Se analizaron 45 muestras de gatos con y sin lesiones dermatológicas, recogidas mediante raspados de piel y depilación, o cepillado de Mackenzie, respectivamente. Las muestras se procesaron con KOH y glicerol tras exposición al calor. El cultivo se realizó durante 30 días en agar inclinado de Sabouraud glucosado y Lactrimel con cloranfenicol y cicloheximida. Resultados. La frecuencia de aislamiento de dermatofitos en este estudio preliminar fue de 13,3%. No se encontraron diferencias estadísticamente significativas en la procedencia, edad, sexo, raza ni estado dermatológico. Se hallaron dermatofitos zoonóticos en 2 animales de compañía de 21 que tenían contacto directo con niños o ancianos. M. canis tuvo una frecuencia de aislamiento de 83,3%. Conclusiones. La frecuencia de aislamiento de dermatofitos zoonóticos en la muestra de felinos del área urbana del Gran Mendoza fue del 13,3%, mayor a la esperada. M. canis fue el dermatofito más frecuentemente aislado(AU)


Background. The cat, considered the main reservoir of Microsporum canis, lives in urban areas, and also plays an important role in the emergence of dermatomycoses. Aims. To determine and analyse the frequency of zoonotic dermatophytes in a sample of cats in an urban area of the Gran Mendoza region. Methods. The animals selected were household cats and cats less than one year old that came from shelters and kennels from urban areas in the Gran Mendoza region. A total of 45 samples from cats with and without dermatological lesions were analysed. These samples were collected through skin scraping, hair removal and Mackenzie brush, respectively. Direct observation was made with KOH and glycerol after heat exposure. Samples were cultured on Sabouraud and Lactrimel agar slants with chloramphenicol and cycloheximide for 30 days. Results. The frequency of dermatophytes isolated in this preliminary study was 13.3%. There were not statistically significant differences by source, age, sex, race or dermatological condition. Zoonotic dermatophytes were found in 2 household cats out of the 21 that had direct contact with children or the elderly. M. canis was isolated in 83.3% cases. Conclusions. The frequency of isolation of zoonotic dermatophytes in the sample of cats in an urban area of the Gran Mendoza region was 13.3%, a value higher than expected. M. canis was the most isolated species(AU)


Assuntos
Animais , Masculino , Feminino , Gatos , Arthrodermataceae/isolamento & purificação , Microsporum/isolamento & purificação , Microsporum/patogenicidade , Micoses/diagnóstico , Micoses/microbiologia , Micoses/veterinária , Dermatomicoses/epidemiologia , Dermatomicoses/prevenção & controle , Dermatomicoses/veterinária , Argentina/epidemiologia , Fungos/isolamento & purificação , Fungos/patogenicidade
17.
MBio ; 3(5): e00259-12, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22951933

RESUMO

The major cause of athlete's foot is Trichophyton rubrum, a dermatophyte or fungal pathogen of human skin. To facilitate molecular analyses of the dermatophytes, we sequenced T. rubrum and four related species, Trichophyton tonsurans, Trichophyton equinum, Microsporum canis, and Microsporum gypseum. These species differ in host range, mating, and disease progression. The dermatophyte genomes are highly colinear yet contain gene family expansions not found in other human-associated fungi. Dermatophyte genomes are enriched for gene families containing the LysM domain, which binds chitin and potentially related carbohydrates. These LysM domains differ in sequence from those in other species in regions of the peptide that could affect substrate binding. The dermatophytes also encode novel sets of fungus-specific kinases with unknown specificity, including nonfunctional pseudokinases, which may inhibit phosphorylation by competing for kinase sites within substrates, acting as allosteric effectors, or acting as scaffolds for signaling. The dermatophytes are also enriched for a large number of enzymes that synthesize secondary metabolites, including dermatophyte-specific genes that could synthesize novel compounds. Finally, dermatophytes are enriched in several classes of proteases that are necessary for fungal growth and nutrient acquisition on keratinized tissues. Despite differences in mating ability, genes involved in mating and meiosis are conserved across species, suggesting the possibility of cryptic mating in species where it has not been previously detected. These genome analyses identify gene families that are important to our understanding of how dermatophytes cause chronic infections, how they interact with epithelial cells, and how they respond to the host immune response.


Assuntos
Arthrodermataceae/genética , Arthrodermataceae/patogenicidade , Microsporum/genética , Microsporum/patogenicidade , Trichophyton/genética , Trichophyton/patogenicidade , Fatores de Virulência/genética , DNA Fúngico/química , DNA Fúngico/genética , Genes Fúngicos , Genoma Fúngico , Humanos , Dados de Sequência Molecular , Análise de Sequência de DNA
18.
Vet Microbiol ; 159(3-4): 479-84, 2012 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-22633172

RESUMO

Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis, mainly in cats, dogs and humans. Proteolytic enzymes have been postulated to be key factors involved in the invasion of the stratum corneum and keratinized epidermal structures. Among these proteases, the secreted subtilisin protease Sub3 was found to be required for adherence of M. canis arthroconidia to feline epidermis. This protease is synthetized as a preproenzyme consisting of a signal peptide followed by the propeptide and the protease domain. In order to assess whether the enzymatic activity of Sub3 could be responsible for the role of the protease in the adherence process, we expressed and characterized the propeptide of Sub3 and demonstrated that this propeptide is a strong inhibitor of its mature enzyme. This propeptide acts as a noncompetitive inhibitor with dissociation constants, K(I) and [Formula: see text] of 170 and 130 nM respectively. When tested for its capacity to inhibit adherence of M. canis to feline epidermis using an ex vivo adherence model made of feline epidermis, the propeptide does not prevent adherence of M. canis arthroconidia because it loses its capacity to inhibit rSub3 following a direct contact with living arthroconidia, presumably through inactivation by fungal membrane-bound proteases.


Assuntos
Doenças do Gato/microbiologia , Dermatomicoses/veterinária , Precursores Enzimáticos/farmacologia , Epiderme/microbiologia , Microsporum/fisiologia , Peptídeo Hidrolases/farmacologia , Animais , Gatos , Dermatomicoses/microbiologia , Dermatomicoses/patologia , Precursores Enzimáticos/genética , Precursores Enzimáticos/metabolismo , Epiderme/patologia , Escherichia coli/genética , Técnicas In Vitro , Microsporum/enzimologia , Microsporum/patogenicidade , Peptídeo Hidrolases/genética , Peptídeo Hidrolases/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/farmacologia , Subtilisina/química
19.
Mycoses ; 55(5): 410-5, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22032519

RESUMO

Dermatophytes are a group of morphologically and physiologically related moulds, which cause well-defined infection called dermatophytosis. The enzymatic ability of fungi to decompose keratin has long been interpreted as a key innovation in the evolution of animal dermatology. In the present study, keratinase activity profile among Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis and Microsporum gypseum isolated on keratin substrates such as human hair, human nail and chicken feather at variable environmental conditions of temperature, pH and metal ions was elucidated. All the above-mentioned fungal strains were isolated from soil using To-KA-Va baiting technique and keratinolytic activity was measured spectrophotometrically. In the temperature range of 30-40 °C and slightly alkaline pH (7.0-8.0), Trichophyton produced the highest activity of keratinase. It can be presumed that high enzyme production of Trichophyton species at normal body temperature range and pH could be an attribute for obligate anthropization in some dermatophytes.


Assuntos
Interações Hospedeiro-Parasita , Microsporum/enzimologia , Peptídeo Hidrolases/metabolismo , Trichophyton/enzimologia , Fatores de Virulência/metabolismo , Animais , Cátions/metabolismo , Galinhas , Plumas/microbiologia , Cabelo/microbiologia , Humanos , Concentração de Íons de Hidrogênio , Metais/metabolismo , Microsporum/crescimento & desenvolvimento , Microsporum/isolamento & purificação , Microsporum/patogenicidade , Unhas/microbiologia , Microbiologia do Solo , Espectrofotometria , Temperatura , Trichophyton/crescimento & desenvolvimento , Trichophyton/isolamento & purificação , Trichophyton/patogenicidade , Virulência
20.
Med Mycol J ; 52(1): 7-13, 2011.
Artigo em Japonês | MEDLINE | ID: mdl-21441707

RESUMO

Tinea capitis is a fungal infection of the scalp. This disease is primarily caused by dermatophytes that invade the hair shaft. The clinical appearance is typically single or multiple lesions of hair loss that may be accompanied by inflammation, scaling, and pustules. The incidence in girls and females overall has recently increased, although many boys were previously affected. Trichophyton or Microsporum species of dermatophytes transmitted by humans or animals are commonly associated with this disease. The treatment requires an oral antifungal agent such as itraconazole or terbinafine.


Assuntos
Tinha do Couro Cabeludo , Administração Oral , Fatores Etários , Animais , Antifúngicos/administração & dosagem , Feminino , Humanos , Itraconazol/administração & dosagem , Japão/epidemiologia , Masculino , Microsporum/isolamento & purificação , Microsporum/patogenicidade , Naftalenos/administração & dosagem , Fatores Sexuais , Terbinafina , Tinha do Couro Cabeludo/tratamento farmacológico , Tinha do Couro Cabeludo/epidemiologia , Tinha do Couro Cabeludo/microbiologia , Tinha do Couro Cabeludo/patologia , Trichophyton/isolamento & purificação , Trichophyton/patogenicidade , Zoonoses
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA