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1.
J Sports Med Phys Fitness ; 59(10): 1709-1715, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31694363

RESUMO

BACKGROUND: The purpose of this study was to investigate the effects of an Ironman-distance triathlon on bone metabolism. METHODS: Nine recreational male triathletes (39.7±8.2 years old) were voluntarily recruited before a 226-km Ironman triathlon race. Baseline blood samples were collected >1 hour before race. Serial post-race blood sampling time points included immediately (0hr), 1 hour (1hr), 1 day (d), 3 d, and 5 d after the Ironman race. RESULTS: Serum muscle damage markers, serum myoglobin, creatine kinase (CK) and lactate dehydrogenase (LDH) revealed significant post-race peak values immediately, 1hr and 1d after the race, respectively. Except for the marginally higher serum CK and myoglobin at 5d (P=0.01~0.05), all post-race serum levels of muscle damage markers were significantly higher than baseline levels (P<0.01). Serum phosphorus values were significantly higher immediately (0hr) after the Ironman race. Serum osteocalcin, an index specific to bone formation, showed a significant decrease at time points 0hr and 1hr, but a significant increase 1 day after (P<0.01) and a marginal increase 3 and 5 days after (P=0.01~0.05) the race. No difference was shown in type I collagen C-telopeptide (CTX-1), a bone resorption marker. Pearson's correlation between serum osteocalcin and CTX-1 was done at each time point, and significant correlation was shown on the 5th d after the race (r=0.591, P<0.05). CONCLUSIONS: An Ironman-distance contest induces a bone-formative-favoring turnover during the post-race period for amateur male triathletes.


Assuntos
Desempenho Atlético , Osso e Ossos/metabolismo , Adulto , Atletas , Ciclismo/fisiologia , Biomarcadores/análise , Creatina Quinase/sangue , Humanos , L-Lactato Desidrogenase/sangue , Masculino , Pessoa de Meia-Idade , Mioglobina/sangue , Resistência Física/fisiologia , Corrida/fisiologia , Natação/fisiologia
2.
Lipids Health Dis ; 18(1): 159, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31391051

RESUMO

BACKGROUND: The study was designed to investigate lipid profile and SYNTAX score in patients with non-ST segment elevation myocardial infarction (NSTEMI). METHODS: 311 patients with NSTEMI were enrolled. The demographic, clinical data, blood samples and SYNTAX score were documented. The Pearson linear correlation was used to detect confounding factors linearly correlated with SYNTAX score. The significantly correlated confounding factors were put into the multiple linear regressions. RESULTS: The Pearson linear correlation showed that high-density lipoprotein- cholesterol (HDL-C) and apolipoprotein A1 (ApoA1) were significantly correlated with Syntax Score (r = - 0.119, P = 0.044 and r = - 0.182, P = 0.002, respectively). The multiple linear regressions for Syntax Score were built using HDL-C and ApoA1, respectively. After the adjustment of other significantly correlated confounding factors such as white blood cell count (WBC), myohemoglobin (MB), glutamic-oxalacetic transaminase (AST) and creatinine, the ApoA1 still showed significant association with Syntax Score (ß = - 0.151, P = 0.028). The area under curve was (AUC) 0.624 and the optimal cutoff value is 1.07 g/L when using ApoA1 to predict moderate and severe coronary artery lesions. The patients with ApoA1 ≥ 1.07 g/L and < 1.07 g/L have the Syntax Scores of 12.21 ± 11.58 and 16.33 ± 11.53, respectively (P = 0.001). CONCLUSIONS: The ApoA1 is the only lipid factor significantly associated with complexity of coronary artery lesion in patients with NSTEMI, the patients with ApoA1 < 1.07 g/L may have more complex coronary artery lesions.


Assuntos
Apolipoproteína A-I/sangue , Infarto do Miocárdio sem Supradesnível do Segmento ST/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Área Sob a Curva , Aspartato Aminotransferases/sangue , Biomarcadores/sangue , HDL-Colesterol/sangue , Creatinina/sangue , Estudos Transversais , Feminino , Humanos , Contagem de Leucócitos , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Mioglobina/sangue , Infarto do Miocárdio sem Supradesnível do Segmento ST/sangue , Infarto do Miocárdio sem Supradesnível do Segmento ST/fisiopatologia , Índice de Gravidade de Doença
3.
Int J Sports Med ; 40(11): 696-703, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31445504

RESUMO

There is a well-conceived notion that rate of recovery from strenuous exercise gets slower with age. However, it is unclear whether older adults who exercise habitually demonstrate slower rates of recovery. We determined whether older adults who are physically active demonstrate slower rates of recovery from unaccustomed strenuous exercise compared with younger peers. Healthy young sedentary (n=10, 28±2 years), young endurance-trained (n=15, 27±2 years), and older endurance-trained (n=14, 58±2 years) men and women were studied. Participants performed 45 min of downhill running at 65% of their maximal oxygen consumption. Visual analog pain scores of muscle groups increased at 24, 48, and 72 h in all three groups (p<0.05), and changes in the muscular pain scale of the legs was smaller in the older trained group than in the young trained group. Maximum isometric strengths at 90° decreased in all groups at 24 h, but the recovery rates were not different at 72 h among the groups. Plasma creatine kinase activity and myoglobin concentration increased at 24 h following downhill running and returned to baseline at 48 h in both the young and older trained groups. The present findings are not consistent with the prevailing notion that older trained adults have a slower rate of recovery from strenuous exercise.


Assuntos
Envelhecimento/fisiologia , Mialgia/fisiopatologia , Resistência Física/fisiologia , Aptidão Física/fisiologia , Corrida/fisiologia , Adulto , Creatina Quinase/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Força Muscular/fisiologia , Músculo Esquelético/lesões , Músculo Esquelético/fisiopatologia , Mialgia/etiologia , Mioglobina/sangue , Consumo de Oxigênio/fisiologia , Amplitude de Movimento Articular , Corrida/lesões
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 223: 117282, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31247463

RESUMO

A sensitive approach based on surface enhanced Raman spectroscopy (SERS) has been developed to evaluate the radiation caused biological injury. To achieve the effective SERS substrate, canonical anodic aluminum oxide (AAO) templates with regular array of nanotips were fabricated, and by plasma sputtering the gold nanoparticles (Au-NPs) were distributed on the nanotips to form the Au-NPs array with plenty of hotspots. The SERS substrates were utilized to examine the serum samples taken from the mice with the treatment of total body irradiation (TBI) of X-ray. The impact of TBI on the mice was analyzed and it was found that the SERS peak intensity at 532 cm-1 increased as a function of duration or dose of TBI. We confirmed that this Raman signature belongs to the myoglobin as a biomarker for the muscle damage due to the radiation caused injury. Furthermore, we also tested several blood and urine specimen of cancer patients who received radiotherapy. The results showed that our approach to some extent could distinguish the bio-samples from normal, X-ray treated and untreated individuals. Therefore, the proposed methodology may have the potential for prompt prognosis of radiation injury at early stage.


Assuntos
Biomarcadores/sangue , Biomarcadores/urina , Ouro/química , Nanopartículas Metálicas/química , Lesões por Radiação/sangue , Lesões por Radiação/urina , Análise Espectral Raman/métodos , Animais , Humanos , Masculino , Camundongos Endogâmicos BALB C , Mioglobina/sangue , Rodaminas/química , Coloração e Rotulagem , Irradiação Corporal Total , Raios X
6.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(5): 594-597, 2019 May.
Artigo em Chinês | MEDLINE | ID: mdl-31198146

RESUMO

OBJECTIVE: To explore the clinical characteristics and early sensitive indicators of severe heat stroke patients in order to predict the development of severe heat stroke in the early stage. METHODS: Thirty-eight patients with severe heat stroke admitted to emergency department of Beijing Daxing District People's Hospital from July 30th to August 5th in 2018 were enrolled. There were 18 patients suffered from exertional heat stroke (EHS), and 12 patients suffered from classical heat stroke (CHS), and 8 patients with heat spasm and heat exhaustion were selected as control group. The gender, age, onset time, body temperature, heart rate (HR), lactic acid (Lac), platelet (PLT), alanine aminotransferase (ALT), alanine aminotransferase (AST), blood urea nitrogen (BUN), serum creatinine (SCr), serum sodium at admission of hospital, as well as positive rate of myoglobin (MYO) and D-dimer (the positive threshold of MYO and D-dimer was 107 µg/L and 600 µg/L respectively) were recorded and compared among the groups. Receiver operating characteristic (ROC) curve was plotted to analyze the prognostic value of MYO and D-dimer on heat stroke. The outcome of all patients was followed up, and the 28-day mortality between EHS and CHS patients was compared. The patient's body temperature was measured again after 4 hours of active cooling treatment (T4 h), and the relationship between T4 h and 28-day mortality was discussed. RESULTS: The majority of severe heat stroke patients were male, especially in EHS patients. EHS patients were younger than CHS ones, and had shorter onset time, with significant differences among the groups. The body temperature and HR at admission in the EHS group and the CHS group were significantly higher than those in the control group [body temperature (centigrade): 41.34±0.67, 40.39±0.58 vs. 37.80±1.39; HR (bpm): 139.78±15.63, 113.08±17.70 vs. 92.00±15.89, all P < 0.05], PLT was significantly lowered (×109/L: 164.94±73.80, 165.78±53.49 vs. 249.50±84.22, both P < 0.05), and the positive rates of MYO and D-dimer were also significantly increased [MYO positive rate: 100.0% (18/18), 100.0% (12/12) vs. 50.0% (4/8); D-dimer positive rate: 77.8% (14/18), 100.0% (12/12) vs. 12.5% (1/8), all P < 0.05]. ROC curve analysis showed that positive MYO and D-dimer at admission had certain diagnostic value for heat stroke, the area under ROC curve (AUC) was 0.750 and 0.871, the sensitivity was 50.0% and 87.5%, and the specificity was 100% and 86.7%, respectively. The 28-day mortality of the EHS group was significantly higher than that of the CHS group [44.4% (8/18) vs. 8.3% (1/12), P < 0.05]. Furthermore, the 28-day mortality of the patients with T4 h ≥ 38 centigrade in the EHS group was significantly higher than those with T4 h < 38 centigrade [70.0% (7/10) vs. 12.5% (1/8), P < 0.05]. CONCLUSIONS: The decreased PLT and the increased D-dimer in the early stage of heat stroke indicate that the damage of coagulation mechanism is prominent in patients with heat stroke. EHS patients have the characteristics of acute onset, severe condition, rapid progression and poor prognosis, and the 28-day mortality is significantly higher than that of CHS patients. MYO and D-dimer are sensitive indicators in early stage of heat stroke patients, which can be used as reference for early diagnosis of heat stroke.


Assuntos
Produtos de Degradação da Fibrina e do Fibrinogênio/análise , Golpe de Calor/diagnóstico , Mioglobina/sangue , Estudos de Casos e Controles , Diagnóstico Precoce , Feminino , Golpe de Calor/sangue , Humanos , Masculino , Valor Preditivo dos Testes , Índice de Gravidade de Doença
7.
Genes (Basel) ; 10(6)2019 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-31208055

RESUMO

Iron is essential for physical activity due to its role in energy production pathways and oxygen transportation via hemoglobin and myoglobin. Changes in iron-related biochemical parameters after physical exercise in athletes are of substantial research interest, but molecular mechanisms such as gene expression are still rarely tested in sports. In this paper, we evaluated the mRNA levels of genes related to iron metabolism (PCBP1, PCBP2, FTL, FTH, and TFRC) in leukocytes of 24 amateur runners at four time points: before, immediately after, 3 h after, and 24 h after a marathon. We measured blood morphology as well as serum concentrations of iron, ferritin, and C-reactive protein (CRP). Our results showed significant changes in gene expression (except for TFRC), serum iron, CRP, and morphology after the marathon. However, the alterations in mRNA and protein levels occurred at different time points (immediately and 3 h post-run, respectively). The levels of circulating ferritin remained stable, whereas the number of transcripts in leukocytes differed significantly. We also showed that running pace might influence mRNA expression. Our results indicated that changes in the mRNA of genes involved in iron metabolism occurred independently of serum iron and ferritin concentrations.


Assuntos
Atletas , Ferritinas/sangue , Ferro/sangue , Corrida/fisiologia , Adulto , Apoferritinas/sangue , Proteína C-Reativa/metabolismo , Proteínas de Ligação a DNA/sangue , Expressão Gênica/genética , Hemoglobinas/metabolismo , Humanos , Leucócitos/metabolismo , Masculino , Pessoa de Meia-Idade , Mioglobina/sangue , Oxirredutases/sangue , RNA Mensageiro/sangue , Proteínas de Ligação a RNA/sangue
8.
Int J Toxicol ; 38(3): 192-201, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31113311

RESUMO

Because dogs are widely used in drug development as nonrodent experimental animals, using a dog model for drug-induced adverse reactions is considered to be relevant for an evaluation and investigation of a mechanism and a biomarker of clinical drug-induced adverse reactions. Skeletal muscle injury occurs by various drugs, including statins and fibrates, during drug development. However, there is almost no report of a dog model for drug-induced skeletal muscle injury. In the present study, we induced skeletal muscle injury in dogs by oral coadministration of lovastatin (LV) and fenofibrate (FF) for 4 weeks. Increases in plasma levels of creatine phosphokinase, myoglobin, miR-1, and miR-133a and degeneration/necrosis of myofibers in skeletal muscles but not in the heart were observed in LV- and FF-coadministered dogs. Plasma levels of lovastatin lactone and lovastatin acid were higher in LV- and FF-coadministered dogs than LV-administered dogs. Taken together, FF coadministration is considered to affect LV metabolism and result in skeletal muscle injury.


Assuntos
Fenofibrato/toxicidade , Hipolipemiantes/toxicidade , Lovastatina/toxicidade , Músculo Esquelético/efeitos dos fármacos , Animais , Creatina Quinase/sangue , Cães , Interações de Medicamentos , Feminino , Fenofibrato/sangue , Fenofibrato/farmacocinética , Hipolipemiantes/sangue , Hipolipemiantes/farmacocinética , Lovastatina/sangue , Lovastatina/farmacocinética , Masculino , MicroRNAs/sangue , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Mioglobina/sangue
9.
Nutrients ; 11(4)2019 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-30935010

RESUMO

Physical performance and regeneration after exercise is enhanced by the ingestion of proteins and carbohydrates. These nutrients are generally consumed by athletes via whey protein and glucose-based shakes. In this study, effects of protein and carbohydrate on skeletal muscle regeneration, given either by shake or by a meal, were compared. 35 subjects performed a 10 km run. After exercise, they ingested nothing (control), a protein/glucose shake (shake) or a combination of white bread and sour milk cheese (food) in a randomized cross over design. Serum glucose (n = 35), serum insulin (n = 35), serum creatine kinase (n = 15) and myoglobin (n = 15), hematologic parameters, cortisol (n = 35), inflammation markers (n = 27) and leg strength (n = 15) as a functional marker were measured. Insulin secretion was significantly stimulated by shake and food. In contrast, only shake resulted in an increase of blood glucose. Food resulted in a decrease of pro, and stimulation of anti-inflammatory serum markers. The exercise induced skeletal muscle damage, indicated by serum creatine kinase and myoglobin, and exercise induced loss of leg strength was decreased by shake and food. Our data indicate that uptake of protein and carbohydrate by shake or food reduces exercise induced skeletal muscle damage and has pro-regenerative effects.


Assuntos
Carboidratos da Dieta/administração & dosagem , Proteínas na Dieta/administração & dosagem , Bebidas Energéticas , Refeições , Músculo Esquelético/metabolismo , Adulto , Atletas , Glicemia/metabolismo , Creatina Quinase/sangue , Estudos Cross-Over , Suplementos Nutricionais , Treino Aeróbico , Exercício/fisiologia , Humanos , Masculino , Mioglobina/sangue , Adulto Jovem
10.
Blood Purif ; 48(2): 167-174, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30943486

RESUMO

BACKGROUND: A novel class of membranes, medium cut-off (MCO) membranes, has recently been designed to achieve interesting removal capacities for middle and large middle molecules in hemodialysis (HD) treatments. The few studies published to date have reported contradictory results regarding middle-sized molecules when comparing MCO dialyzers versus dialyzers used in online hemodiafiltration (OL-HDF). METHODS: A prospective, single-center study was carried out in 22 patients. Each patient underwent 9 dialysis sessions with routine dialysis parameters, one with an MCO dialyzer in HD and the other 8 with different dialyzers in OL-HDF. The removal ratio (RR) of urea, creatinine, ß2-microglobulin, myoglobin, prolactin, α1-microglobulin, α1-acid glycoprotein, and albumin was intraindividually compared. Albumin loss in dialysate was measured. We propose a global removal score ([ureaRR + ß2-microglobulinRR + myoglobinRR + prolactinRR + α1-microglobulinRR + α1-acid glycoproteinRR]/6 - albuminRR) as a new tool for measuring dialyzer effectiveness. RESULTS: No significant differences in the RRs of small and middle molecular range molecules were observed between the MCO vs. OL-HDF dialyzers (range 60-80%). Lower RRs were found for α1-microglobulin and α1-acid glycoprotein without significant differences. The albumin RR was < 11% and dialysate albumin loss was < 3.5 g in all situations without significant differences. The global removal score was 54.9 ± 4.8% with the MCO dialyzer without significant differences. CONCLUSIONS: Removal of a wide range of molecular weights, calculated with the proposed global removal score, was almost equal with the MCO dialyzer in HD treatment compared with 8 high-flux dialyzers in high-volume OL-HDF without relevant changes in albumin loss. The global removal score could be a new tool to evaluate the effectiveness of dialyzers and/or different treatment modalities.


Assuntos
Hemodiafiltração/instrumentação , Falência Renal Crônica/sangue , Falência Renal Crônica/terapia , Diálise Renal/instrumentação , Adulto , Idoso , Idoso de 80 Anos ou mais , alfa-Globulinas/análise , alfa-Globulinas/isolamento & purificação , Creatinina/sangue , Creatinina/isolamento & purificação , Feminino , Hemodiafiltração/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Mioglobina/sangue , Mioglobina/isolamento & purificação , Estudos Prospectivos , Diálise Renal/métodos , Albumina Sérica/análise , Albumina Sérica/isolamento & purificação , Ureia/sangue , Ureia/isolamento & purificação , Adulto Jovem , Microglobulina beta-2/sangue , Microglobulina beta-2/isolamento & purificação
11.
Chem Commun (Camb) ; 55(19): 2829-2832, 2019 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-30766986

RESUMO

A novel electrochemiluminescence resonance energy transfer (ECL-RET) system using versatile gold nanorods as energy acceptors was introduced into the ECL biochemical analysis. A spatial- and potential-resolved platform coupled with the ECL-RET strategy was developed for simultaneous determination of two acute myocardial infarction markers.


Assuntos
Biomarcadores Tumorais/sangue , Transferência Ressonante de Energia de Fluorescência , Ouro/química , Infarto do Miocárdio/diagnóstico , Nanotubos/química , 2,2'-Dipiridil/análogos & derivados , 2,2'-Dipiridil/química , Anticorpos Imobilizados/química , Anticorpos Imobilizados/imunologia , Compostos de Cádmio/química , Humanos , Mioglobina/sangue , Mioglobina/imunologia , Nanofios/química , Compostos Organometálicos/química , Sulfetos/química , Troponina I/sangue , Troponina I/imunologia
12.
Int J Sport Nutr Exerc Metab ; 29(4): 426­434, 2019 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-30632413

RESUMO

Recent studies demonstrate that protein ingestion immediately before sleep improves muscle recovery during the night following resistance exercise. Whether this feeding strategy benefits recovery from endurance training has yet to be established. The aim of this study was to investigate the effects of whey protein isolate ingested every night before sleep on subsequent performance and circulatory markers of muscular recovery during a week of intensified endurance training mimicking a training camp. In a parallel design, 32 trained runners underwent a 1-week intervention with a rigorously controlled diet (carbohydrate = 7.2 g·kg-1·day-1, protein = 1.8 g·kg-1·day-1, and fat = 1.0 g·kg-1·day-1) and exercise program (11 sessions) while receiving either a protein (0.5 g·kg-1·day-1) or carbohydrate (0.5 g·kg-1·day-1) beverage every night before sleep. Blood samples were obtained on the morning of Days 1, 4, 7, and 8 and analyzed for markers of muscle damage (creatine kinase, lactate dehydrogenase, and myoglobin). The postintervention 5-km time-trial performance was significantly impaired in both groups (11 ± 24 s, p < .01). Plasma creatine kinase (227% ± 221%, p < .01), lactate dehydrogenase (18% ± 22%, p < .01), and myoglobin (72% ± 62%, p < .01) increased gradually throughout the week with no difference between the groups (p > .05). In conclusion, the presleep protein ingestion did not reduce the decline in performance or ameliorate the rise of circulatory markers of muscle damage during a week of intensified training when compared with the isocaloric carbohydrate ingestion.


Assuntos
Desempenho Atlético , Suplementos Nutricionais , Treino Aeróbico , Sono , Fenômenos Fisiológicos da Nutrição Esportiva , Proteínas do Soro do Leite/administração & dosagem , Adulto , Creatina Quinase/sangue , Carboidratos da Dieta , Método Duplo-Cego , Humanos , L-Lactato Desidrogenase/sangue , Masculino , Mioglobina/sangue , Corrida , Adulto Jovem
13.
PLoS One ; 14(1): e0210651, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30657773

RESUMO

The aim of the paper was to assess indicators of muscle and intestinal damage in triathletes. The study involved 15 triathletes whose objective for the season was to start in the XTERRA POLAND 2017 event (1,500-m swimming, 36-km cycling, and 10-km mountain running). Before the 14-week preparatory period, the competitors' body composition was measured, aerobic capacity was tested (graded treadmill test) and blood samples were collected to determine markers showing the level of muscle and intestinal damage. Subsequent tests for body composition were carried out before and after the competition. Blood samples for biochemical indicators were collected the day before the competition, after the completed race, and 24 and 48 hours later. A significant decrease in body mass was observed after completing the race (-3.1±1.5%). The mean maximal oxygen uptake level among the studied athletes equalled 4.9±0.4 L·min-1, 58.8±4.5 mL·kg-1·min-1. The significant increase in concentrations of cortisol, c-reactive protein and myoglobin after the competition, significantly correlated with the significant increase in zonulin concentration (post 1h: r = 0.88, p = 0.007, r = 0,79, p = 0.001, r = 0.78, p = 0.001, and post 12h: r = 0.75, p = 0.01, r = 0.71, p = 0.011, r = 0.83, p = 0.02). No significant changes in the concentration of tumour necrosis factor alpha among the examined competitors were noted at following stages of the study. The results of our research showed that in order to monitor overload in the training of triathletes, useful markers reflecting the degree of muscle and intestinal damage include cortisol, testosterone, testosterone to cortisol ratio, c-reactive protein, myoglobin and zonulin. Changes in muscle cell damage markers strongly correlated with changes in zonulin concentration at particular stages of the study. Thus, one can expect that the concentrations of markers depicting the level of muscle cell damage after an intense and long-lasting effort will significantly influence the level of the intestinal barrier.


Assuntos
Ciclismo/fisiologia , Intestinos/fisiologia , Músculo Esquelético/metabolismo , Músculo Esquelético/fisiologia , Corrida/fisiologia , Natação/fisiologia , Adulto , Atletas , Biomarcadores/sangue , Composição Corporal/fisiologia , Proteína C-Reativa/metabolismo , Toxina da Cólera/sangue , Tolerância ao Exercício/fisiologia , Feminino , Humanos , Hidrocortisona/sangue , Masculino , Mioglobina/sangue , Consumo de Oxigênio/fisiologia
14.
Scand J Med Sci Sports ; 29(5): 725-735, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30663816

RESUMO

This study compared nine resistance eccentric exercises targeting arm, leg, and trunk muscles in one session for changes in maximal voluntary isometric contraction strength (MVC), delayed onset muscle soreness (DOMS), plasma creatine kinase (CK) activity, and myoglobin (Mb) concentration after the first and second bouts. Fifteen sedentary men (20-25 years) performed 5 sets of 10 eccentric contractions with 80% of MVC load for the elbow flexors (EF), elbow extensors (EE), pectoralis, knee extensors (KE), knee flexors (KF), plantar flexors (PF), latissimus, abdominis, and erector spinae (ES) in a randomized order and repeated the same exercises 2 weeks later. MVC decreased at 1 (16%-57%) to 4 (13%-49%) days, DOMS developed (peak: 43-70 mm), and CK activity (peak: 23 238-207 304 IU/L) and Mb concentration showed large increases after the first bout. The magnitude of decrease in MVC was greater (P < 0.05) for EF, EE, and PEC than others and for KF than KE, PF, and ES. DOMS was greater (P < 0.05) for EF, EE, and ES than others. Changes in all measures were smaller (P < 0.05) after the second than the first bout, and the magnitude of the repeated bout effect was similar among the muscles. Plasma CK activity and Mb concentration did not increase significantly after the second exercise bout. It was concluded that muscle damage was greater for arm than leg muscles, and muscle proteins in the blood increased to a critical level after unaccustomed whole-body resistance exercises, but the magnitude of damage was largely attenuated for all muscles similarly after the second bout.


Assuntos
Contração Isométrica , Força Muscular , Músculo Esquelético/fisiologia , Mialgia , Treinamento de Resistência , Adulto , Braço , Creatina Quinase/sangue , Humanos , Perna (Membro) , Masculino , Mioglobina/sangue , Tronco , Adulto Jovem
15.
Biosens Bioelectron ; 128: 176-185, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30685097

RESUMO

The early detection of acute myocardial infarction (AMI) upon the onset of chest pain symptoms is crucial for patient survival. However, this detection is challenging, particularly without a persistent elevation of ST-segment reflected in an electrocardiogram or in blood tests. A majority of the available point-of-care testing devices allow accurate and rapid diagnosis of AMI. However, AMI diagnosis is reliable only at intermediate and later stages, with myocardial injury (> 6 h) and MI, based on the expression of specific cardiac biomarkers including troponin I or T (cTnI or cTnT), creatine kinase-MB (CK-MB), and myoglobin. Diagnosis at the early myocardial ischemia stage is not possible. To overcome this limitation, a sensitive and rapid microfluidic paper-based device (µPAD) was developed for the simultaneous detection of multiple cardiac biomarkers for the early and late diagnosis of AMI. The glycogen phosphorylase isoenzyme BB (GPBB) was detected during early (within first 4 h) ischemic myocardial injury. On the same µPAD platform, detection of prolonged elevation of levels of cTnT and CK-MB, which are only produced 6 h after the onset of chest pain in human serum, was possible. Sandwich immunoassay performed on the µPAD achieved reproducibility (RSD approximately 10% and intra-and inter-day precision (CV 10-20%, 99th percentile), as well as consistently stable test results for 28 days, with strong correlation (r2= 0.962), using the standard Siemens Centaur XPT Immunoassay system. The present findings indicate the potential of the µPAD platform as a point-of-care device for the early diagnosis and prognosis of AMI.


Assuntos
Biomarcadores/sangue , Técnicas Biossensoriais , Diagnóstico Precoce , Infarto do Miocárdio/sangue , Creatina Quinase Forma MB/sangue , Creatina Quinase Forma MB/isolamento & purificação , Humanos , Dispositivos Lab-On-A-Chip , Miocárdio/patologia , Mioglobina/sangue , Sistemas Automatizados de Assistência Junto ao Leito , Prognóstico , Troponina I/sangue , Troponina T/sangue , Troponina T/isolamento & purificação
16.
Biosens Bioelectron ; 129: 87-92, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30685706

RESUMO

A simple, sensitive, quantitative point-of-care testing (POCT) was developed integrating enzyme-linked DNA supersandwich amplification with optical fiber amplifier. The point-of-care (POC) assay can work in both "Turn-off" mode and "Turn-on" mode. Using myoglobin (Myo) and miRNA-141 as the model biomarker, as low as 0.5 nM Myo and 10 pM miRNA-141 could be detected. More importantly, the optical fiber amplifier, which was used as signal readout, exhibited low-cost, small size, rapid response, and easy operation. Although it did not need large equipment and professional technical personnel, the assay was successfully applied to detect Myo and miRNA-141 in 40% human serum. Since the entire process of the strategy could be done directly in the centrifugal tube, requiring a few easy steps, it showed great potential application for early diagnosis in the world, especially in developing countries or remote regions. This work provided a new avenue for developing the portable and sensitive biosensor.


Assuntos
Técnicas Biossensoriais/instrumentação , Tecnologia de Fibra Óptica/instrumentação , MicroRNAs/sangue , Mioglobina/sangue , Testes Imediatos , Técnicas Biossensoriais/economia , Desenho de Equipamento , Tecnologia de Fibra Óptica/economia , Humanos , Limite de Detecção , Fibras Ópticas , Testes Imediatos/economia
17.
BMC Musculoskelet Disord ; 20(1): 15, 2019 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-30611244

RESUMO

BACKGROUND: The intense ischemic pain of acute compartment syndrome can be difficult to discriminate from the pain related to an associated fracture. Lacking objective measures, the decision to perform fasciotomy is often only based on clinical findings and performed at a low threshold. Biomarkers of muscle cell damage might help to identify and monitor patients at risk. In patients with fractures, however, markers of muscle cell damage could be elevated because of other reasons associated with the trauma, which would make interpretation difficult. In a review of all patients who underwent emergency fasciotomy in our health care district we aimed to investigate the decision-making process and specifically the use of biomarkers in patients with and without fractures. METHODS: In the southeast health care region of Sweden 79 patients (60 men) with fractures (median age 26 years) and 42 patients (34 men) without associated fractures (median age 44 years) were treated with emergency fasciotomy of the lower leg between 2007 and 2016. Differences in clinical findings, p-myoglobin and p-creatine phosphokinase as well as pressure measurements were investigated. RESULTS: P-myoglobin was analyzed preoperatively in 20% of all cases and p-creatine phosphokinase in 8%. Preoperative levels of p-myoglobin were lower in patients with fractures (median 1065 µg/L, range 200-3700 µg/L) compared with those without fractures (median 7450 µg/L, range 29-31,000 µg/L), p < 0.05. Preoperative intracompartmental pressure was lower in the fracture group (median 45 mmHg, range 25-90 mmHg) compared with those without fractures (median 83 mmHg, range 18-130 mmHg), p < 0.05. CONCLUSIONS: Biomarkers are seldom used in the context of acute fasciotomy of the lower leg. Contrary to our expectations, preoperative levels of p-myoglobin and intracompartmental pressures were lower in fracture patients. These findings support differences in the underlying pathomechanism between the groups and indicate that biomarkers of muscle cell necrosis might play a more important role in the diagnosis of acute compartment syndrome than previously thought.


Assuntos
Síndromes Compartimentais/diagnóstico , Mioglobina/sangue , Fraturas da Tíbia/diagnóstico , Doença Aguda , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/sangue , Tomada de Decisão Clínica , Síndromes Compartimentais/sangue , Síndromes Compartimentais/fisiopatologia , Síndromes Compartimentais/cirurgia , Creatina Quinase/sangue , Descompressão Cirúrgica/métodos , Fasciotomia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medição da Dor , Seleção de Pacientes , Valor Preditivo dos Testes , Pressão , Suécia , Fraturas da Tíbia/sangue , Fraturas da Tíbia/fisiopatologia , Adulto Jovem
18.
Vasc Health Risk Manag ; 15: 1-10, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30697054

RESUMO

Purpose: Acute myocardial infarction (AMI) is the most common cause of death in the world. Comprehensive risk assessment of patients presenting with chest pain and eliminating undesirable results should decrease morbidity and mortality rates, increase the quality of life of patients, and decrease health expenditure in many countries. In this study, the advantages and disadvantages of the enzymatic and nonenzymatic biomarkers used in the diagnosis of patients with AMI are given in historical sequence, and some candidate biomarkers - hFABP, GPBB, S100, PAPP-A, RP, TNF, IL6, IL18, CD40 ligand, MPO, MMP9, cell-adhesion molecules, oxidized LDL, glutathione, homocysteine, fibrinogen, and D-dimer procalcitonin - with a possible role in the diagnosis of AMI are discussed. Methods: The present study was carried out using meta-analyses, reviews of clinical trials, evidence-based medicine, and guidelines indexed in PubMed and Web of Science. Results: These numerous AMI biomarkers guide clinical applications (diagnostic methods, risk stratification, and treatment). Today, however, TnI remains the gold standard for the diagnosis of AMI. Details in the text will be given of many biomarkers for the diagnosis of AMI. Conclusion: We evaluated the advantages and disadvantages of routine enzymatic and nonenzymatic biomarkers and the literature evidence of other candidate biomarkers in the diagnosis of AMI, and discuss challenges and constraints that limit translational use from bench to bedside.


Assuntos
Biomarcadores/sangue , Infarto do Miocárdio/sangue , Creatina Quinase Forma MB/sangue , Humanos , Infarto do Miocárdio/diagnóstico , Infarto do Miocárdio/mortalidade , Infarto do Miocárdio/terapia , Mioglobina/sangue , Valor Preditivo dos Testes , Prognóstico , Reprodutibilidade dos Testes , Fatores de Risco , Troponina I/sangue , Troponina T/sangue
19.
J Sports Med Phys Fitness ; 59(4): 581-586, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29619805

RESUMO

BACKGROUND: It is well known that eccentric muscle contractions induce delayed onset muscle soreness (DOMS) and exertional rhabdomyolysis (ERB), both of which are related to high blood levels of muscle damage markers after exercise. Creatine kinase (CK) is, together with some other markers (i.e. myoglobin, α-actin, skeletal troponin), widely used in determination of muscle damage. Moreover, DOMS after eccentric exercise has been shown to be associated with altered blood lactate concentration after subsequent submaximal or maximal cycling exercise. However, it is unknown whether blood lactate can predict muscle damage levels after maximal stretch-shortening cycle (SSC) exercise, as due to the differences in types of contractions between the eccentric and SSC protocol. Additionally, we determined the association between blood lactate concentration and CMJ performance after such a protocol. METHODS: Forty-three healthy, physically active young men completed a plyometric training session consisting of maximal countermovement jumps (CMJ) to failure. The blood creatine kinase (CK), myoglobin (Mb) and aspartate aminotransferase (AST) samples were taken pre- and 48 hours post-exercise. Blood lactate concentration was determined directly post-exercise. RESULTS: There was a significant correlation between blood lactate concentration (post) and blood CK (P=0.027), Mb (P=0.007) and AST (P=0.024) (48 hours post), which means that higher blood lactate concentration is associated with higher muscle damage values after exercise. No correlation was found between blood lactate concentration (post) and performance loss which was expressed by the decrease in maximum CMJ height 5 minutes post-exercise. CONCLUSIONS: Blood lactate concentration could be used as a predictor of muscle damage levels after maximal SSC exercise, which supports the results found in other studies, although after eccentric exercise. However, it cannot predict acute performance loss in jump height.


Assuntos
Desempenho Atlético/fisiologia , Ácido Láctico/sangue , Contração Muscular , Músculo Esquelético/fisiologia , Exercício Pliométrico , Adulto , Biomarcadores/sangue , Creatina Quinase/sangue , Humanos , Masculino , Mialgia , Mioglobina/sangue , Rabdomiólise , Adulto Jovem
20.
Cytokine ; 113: 277-284, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30031682

RESUMO

PURPOSE: This study examined the acute effects of a single session of Whole-body Cryotherapy (WBC) following severe intermittent running exercise on biomarkers of inflammation, muscle damage and stress. METHODS: Endurance-trained males (n = 11) were tested twice using a within-participant, balanced cross-over design that consisted of 5 × 5 min of high-intensity running (HIR) followed by either 3 min of WBC at -110 °C or a passive control condition (CON). Before the HIR and after 60 min of recovery a ramp-test was completed. At seven time points up to 24 hrs post exercise venous blood samples were analyzed for serum levels of interleukin 6 (IL-6), interleukin 10 (IL-10), c-reactive protein (CRP), soluble intercellular adhesion molecule-1 (sICAM-1), myoglobin, cortisol, and testosterone. RESULTS: HIR induced significant increases in all biomarkers except sICAM-1 in both recovery conditions, respectively. Compared to the CON condition WBC did not attenuate exercise- induced changes in IL-6, IL-10, sICAM-1, myoglobin, cortisol, testosterone or their ratio. Increased levels of cortisol following exercise were negatively correlated with subsequent running performance in both conditions (WBC: r = -0.61, p = 0.04; CON: r = -0.64, p = 0.04). CONCLUSION: The results of this study suggest that the postulated physiological mechanisms by which WBC is proposed to improve recovery, i.e. reductions in inflammation and muscle damage, may not be accurate.


Assuntos
Proteína C-Reativa/metabolismo , Crioterapia , Exercício , Hidrocortisona/sangue , Molécula 1 de Adesão Intercelular/sangue , Interleucina-10/sangue , Interleucina-6/sangue , Músculo Esquelético/lesões , Mioglobina/sangue , Testosterona/sangue , Adulto , Biomarcadores/sangue , Humanos , Masculino
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