Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 92
Filtrar
1.
Acta Virol ; 63(3): 333-337, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31507201

RESUMO

Molluscum contagiosum is a common, self-limiting infectious disease of the skin caused by molluscum contagiosum virus (MCV). The disease primarily affects children, sexually active adults, and immunocompromised individuals. Transmission of the virus occurs by direct skin contact. Therefore, the virus is usually detected in the skin and genitals of patients. However, the diagnosis of intracranial infection by the virus is difficult if the skin/mucosa lessons are atypical or absent, and the presence of the virus in the cerebrospinal fluid has not been reported. We report a very rare case of intracranial infection by molluscum contagiosum virus. A 25-year-old girl was admitted to our hospital due to severe headache but no fever or other symptoms. Upon examination, some small flesh-colored flattened papules on both arms were noticed. Blood tests showed slightly reduced levels of CD3 and CD4 T lymphocytes. Three-dimensional time-of-flight magnetic resonance angiography (3D-TOF-MRA) and head magnetic resonance (MR) were both normal. Lumbar puncture was performed, and metagenomic sequencing was applied to the spinal fluid. The unique sequences of the molluscum contagiosum virus were identified in the fluid. The patient was then diagnosed with intracranial molluscum contagiosum virus infection. No special treatment was given. The headache gradually disappeared, and the patient was discharged. During our quarterly follow-up, the girl appeared normal, and her skin lesions disappeared. However, her CD3 and CD4 T lymphocyte counts were still slightly lower than the normal level. Our case shows that the application of metagenomic sequencing to cerebrospinal fluid is a sensitive and powerful means to detect pathogens causing intracranial infection. Keywords: Molluscum contagiosum; intracranial infection; metagenomics sequencing.


Assuntos
Metagenômica , Molusco Contagioso , Vírus do Molusco Contagioso , Adulto , Linfócitos T CD4-Positivos/citologia , Feminino , Humanos , Contagem de Linfócitos , Molusco Contagioso/líquido cefalorraquidiano , Molusco Contagioso/diagnóstico , Molusco Contagioso/imunologia , Vírus do Molusco Contagioso/genética , Pele/virologia
2.
PLoS Pathog ; 15(4): e1007711, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31034515

RESUMO

The human specific poxvirus molluscum contagiosum virus (MCV) produces skin lesions that can persist with minimal inflammation, suggesting that the virus has developed robust immune evasion strategies. However, investigations into the underlying mechanisms of MCV pathogenesis have been hindered by the lack of a model system to propagate the virus. Herein we demonstrate that MCV-encoded MC80 can disrupt MHC-I antigen presentation in human and mouse cells. MC80 shares moderate sequence-similarity with MHC-I and we find that it associates with components of the peptide-loading complex. Expression of MC80 results in ER-retention of host MHC-I and thereby reduced cell surface presentation. MC80 accomplishes this by engaging tapasin via its luminal domain, targeting it for ubiquitination and ER-associated degradation in a process dependent on the MC80 transmembrane region and cytoplasmic tail. Tapasin degradation is accompanied by a loss of TAP, which limits MHC-I access to cytosolic peptides. Our findings reveal a unique mechanism by which MCV undermines adaptive immune surveillance.


Assuntos
Apresentação do Antígeno/imunologia , Degradação Associada com o Retículo Endoplasmático , Retículo Endoplasmático/metabolismo , Antígenos de Histocompatibilidade Classe I/imunologia , Proteínas de Membrana Transportadoras/metabolismo , Molusco Contagioso/imunologia , Vírus do Molusco Contagioso/imunologia , Proteínas Virais/metabolismo , Membro 2 da Subfamília B de Transportadores de Cassetes de Ligação de ATP/genética , Membro 2 da Subfamília B de Transportadores de Cassetes de Ligação de ATP/metabolismo , Animais , Células Cultivadas , Humanos , Evasão da Resposta Imune , Camundongos , Molusco Contagioso/metabolismo , Molusco Contagioso/virologia , Linfócitos T Citotóxicos/imunologia , Proteínas Virais/genética
3.
Int J Dermatol ; 58(10): 1165-1171, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30927252

RESUMO

BACKGROUND: Human immunodeficiency virus (HIV) infection in children is becoming a common occurrence. Worldwide, limited studies have been done on the mucocutaneous manifestations in HIV-positive children. The aim of our study was to analyze the spectrum of mucocutaneous manifestations of pediatric HIV infection and correlate to degree of immunosuppression. MATERIAL AND METHODS: One hundred and sixty-five children under 18 years with HIV, who presented to the departments of dermatology and pediatrics, were examined for mucocutaneous manifestations. Patients were classified into four groups of immunodeficiency such as normal, mild, advanced, and severe, based on NACO guidelines of immunosuppression. The most recent CD4 count (within 6 months of study period) was considered. RESULTS: One hundred and sixty-five patients were examined, and skin manifestations were seen in 100 (61%) of them.The highest incidence of mucocutaneous manifestations was in 6-10 age group. Papular pruritic eruptions (PPE) (16%) was the most common condition, with highest prevalence in severe CD4 category (38%). Molluscum contagiosum (MC) (10%) was the most common infectious condition, with highest prevalence in advanced CD4 category (14%). Severe cutaneous adverse reactions (SCAR) caused by nevirapine were seen in three children. The percentage of skin manifestations was highest in the advanced (107%) and severe (100%) CD4 category. There was no significant difference in manifestations between those who were on antiretroviral therapy (ART) and those not. CONCLUSION: The percentage of skin manifestations increased with degree of CD4 depletion. PPE was found to be the hallmark of severe immunosuppression. However, opportunistic infections did not correlate with severity of immunodeficiency.


Assuntos
Erupção por Droga/epidemiologia , Infecções por HIV/complicações , Tolerância Imunológica , Molusco Contagioso/epidemiologia , Infecções Oportunistas/epidemiologia , Adolescente , Contagem de Linfócito CD4 , Linfócitos T CD4-Positivos/imunologia , Criança , Pré-Escolar , Erupção por Droga/complicações , Erupção por Droga/imunologia , Feminino , Infecções por HIV/diagnóstico , Infecções por HIV/imunologia , Humanos , Incidência , Índia/epidemiologia , Masculino , Molusco Contagioso/complicações , Molusco Contagioso/imunologia , Nevirapina/efeitos adversos , Infecções Oportunistas/complicações , Infecções Oportunistas/imunologia , Prevalência , Índice de Gravidade de Doença
4.
Viruses ; 10(11)2018 10 26.
Artigo em Inglês | MEDLINE | ID: mdl-30373153

RESUMO

Molluscum contagiosum virus (MCV) is the sole member of the Molluscipoxvirus genus and the causative agent of molluscum contagiosum (MC), a common skin disease. Although it is an important and frequent human pathogen, its genetic landscape and evolutionary history remain largely unknown. In this study, ten novel complete MCV genome sequences of the two most common MCV genotypes were determined (five MCV1 and five MCV2 sequences) and analyzed together with all MCV complete genomes previously deposited in freely accessible sequence repositories (four MCV1 and a single MCV2). In comparison to MCV1, a higher degree of nucleotide sequence conservation was observed among MCV2 genomes. Large-scale recombination events were identified in two newly assembled MCV1 genomes and one MCV2 genome. One recombination event was located in a newly identified recombinant region of the viral genome, and all previously described recombinant regions were re-identified in at least one novel MCV genome. MCV genes comprising the identified recombinant segments have been previously associated with viral interference with host T-cell and NK-cell immune responses. In conclusion, the two most common MCV genotypes emerged along divergent evolutionary pathways from a common ancestor, and the differences in the heterogeneity of MCV1 and MCV2 populations may be attributed to the strictness of the constraints imposed by the host immune response.


Assuntos
Genoma Viral , Genômica , Molusco Contagioso/virologia , Vírus do Molusco Contagioso/genética , Quimiotaxia/imunologia , Biologia Computacional/métodos , Evolução Molecular , Variação Genética , Genômica/métodos , Genótipo , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Imunidade , Células Matadoras Naturais/imunologia , Células Matadoras Naturais/metabolismo , Anotação de Sequência Molecular , Molusco Contagioso/imunologia , Vírus do Molusco Contagioso/imunologia , Mosaicismo , Filogenia , Recombinação Genética , Linfócitos T/imunologia , Linfócitos T/metabolismo , Carga Viral
5.
Cutis ; 101(2): 136-140, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29554156

RESUMO

Molluscum contagiosum (MC) is a common, self-limited cutaneous infection in immunocompetent individuals. However, in immunocompromised individuals the infection often has an atypical presentation and can be difficult to eradicate, making both the diagnosis and treatment challenging. Due to advancements in the management of patients with human immunodeficiency virus (HIV) and cancer, there is a growing population of immunosuppressed individuals, signaling the need for dermatologists to recognize and manage related skin diseases. We present a case of an atypical MC eruption in a patient on biologic therapy for psoriasis and an unrecognized underlying HIV infection, followed by a current review of the presentation and treatment of MC in various immunosuppressed states. With a growing population of immunosuppressed patients, it is important to recognize MC as a potential indicator of underlying immunosuppression. Testing for HIV should be offered to any patient starting immunosuppressive therapy such as biologic agents.


Assuntos
Síndrome de Imunodeficiência Adquirida/diagnóstico , Síndrome de Imunodeficiência Adquirida/imunologia , Terapia Biológica/efeitos adversos , Hospedeiro Imunocomprometido , Molusco Contagioso/etiologia , Psoríase/terapia , Infecções Oportunistas Relacionadas com a AIDS/etiologia , Adulto , Humanos , Masculino , Molusco Contagioso/imunologia
6.
JAMA Dermatol ; 154(2): 203-204, 2018 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-29282454

RESUMO

Clinical Question: How effective and safe are treatments for nongenital molluscum contagiosum in persons without immune deficiency? Bottom Line: Compared with placebo, imiquimod, 5%, cream was not more effective in clearing molluscum contagiosum and caused more local adverse effects, including application site reactions, in children aged 2 to 12 years; because high-quality evidence for other interventions is lacking, allowing for natural resolution of the infection remains a reasonable approach.


Assuntos
Imiquimode/uso terapêutico , Imunocompetência , Molusco Contagioso/tratamento farmacológico , Molusco Contagioso/imunologia , Criança , Pré-Escolar , Relação Dose-Resposta a Droga , Esquema de Medicação , Feminino , Seguimentos , Humanos , Masculino , Molusco Contagioso/diagnóstico , Ensaios Clínicos Controlados Aleatórios como Assunto , Valores de Referência , Índice de Gravidade de Doença , Creme para a Pele , Resultado do Tratamento
8.
Ann Allergy Asthma Immunol ; 119(5): 446-451, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28866311

RESUMO

BACKGROUND: Although mutations in the filaggrin (FLG) gene have been reported to predispose patients with atopic dermatitis (AD) skin infection susceptibility, to date, the data reported in the literature are still controversial. OBJECTIVE: To evaluate the role of FLG polymorphisms expression and risk of developing a concomitant Molluscum contagiosum sustained skin infection in the pediatric population with AD. METHODS: A total of 100 children with AD and 97 healthy children were enrolled. AD was diagnosed and assessed according to the validated European Task Force on Atopic Dermatitis. DNA samples of patients were analyzed for allelic variants in the promoter and coding exon of FLG. Genotyping was performed with polymerase chain reaction amplification and direct sequencing. RESULTS: Sixteen FLG variants have been detected in 29% of patients with AD: 2 synonymous (rs79808464 and rs116222149), 12 missense (rs11584340, rs113136594, rs145828067, rs374910442, rs747005144, rs145627745, rs144209313, rs74129443, rs192455877, rs150957860, rs138055273, rs147472105), 1 stop gained (rs183942200), and 1 frameshift (rs 558269137). In contrast, only 13% of the control group reported FLG mutations (22 heterozygous variants). In addition, the age at disease onset correlated significantly with FLG variants (P < .001). In addition, the AD with FLG gene variants (rs145627745, rs79808464, rs150957860, rs145828067, rs747005144, rs374910442, rs138055273, rs183942200, rs11584340, and rs113136594) reported moderate to severe Scoring Atopic Dermatitis scores. Finally, the AD group and the AD plus M contagiosum skin infection group had a significant association with FLG mutations when compared with the control group (P < .01). CONCLUSION: FLG mutations are associated with early onset of AD, more severe clinical course of disease, and a significantly increased risk of M contagiosum sustained skin infection.


Assuntos
Dermatite Atópica/genética , Grupo com Ancestrais do Continente Europeu , Proteínas de Filamentos Intermediários/genética , Mutação/genética , Pele/imunologia , Criança , Pré-Escolar , Dermatite Atópica/imunologia , Feminino , Predisposição Genética para Doença , Genótipo , Humanos , Proteínas de Filamentos Intermediários/metabolismo , Masculino , Molusco Contagioso/imunologia , Fenótipo , Polimorfismo de Nucleotídeo Único , Pele/virologia
9.
Virus Genes ; 53(4): 522-531, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28425034

RESUMO

The molluscum contagiosum virus (MCV) uses a variety of immune evasion strategies to antagonize host immune responses. Two MCV proteins, MC159 and MC160, contain tandem death effector domains (DEDs). They are reported to inhibit innate immune signaling events such as NF-κB and IRF3 activation, and apoptosis. The RxDL motif of MC159 is required for inhibition of both apoptosis and NF-κB activation. However, the role of the conserved RxDL motif in the MC160 DEDs remained unknown. To answer this question, we performed alanine mutations to neutralize the arginine and aspartate residues present in the MC160 RxDL in both DED1 and DED2. These mutations were further modeled against the structure of the MC159 protein. Surprisingly, the RxDL motif was not required for MC160's ability to inhibit MAVS-induced IFNß activation. Further, unlike previous results with the MC159 protein, mutations within the RxDL motif of MC160 had no effect on the ability of MC160 to dampen TNF-α-induced NF-κB activation. Molecular modeling predictions revealed no overall changes to the structure in the MC160 protein when the amino acids of both RxDL motifs were mutated to alanine (DED1 = R67A D69A; DED2 = R160A D162A). Taken together, our results demonstrate that the RxDL motifs present in the MC160 DEDs are not required for known functions of the viral protein.


Assuntos
Evasão da Resposta Imune , Molusco Contagioso/virologia , Vírus do Molusco Contagioso/imunologia , Proteínas Virais/química , Proteínas Virais/imunologia , Motivos de Aminoácidos , Apoptose , Humanos , Interferon beta/genética , Interferon beta/imunologia , Molusco Contagioso/genética , Molusco Contagioso/imunologia , Molusco Contagioso/fisiopatologia , Vírus do Molusco Contagioso/química , Vírus do Molusco Contagioso/genética , Domínios Proteicos , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologia , Proteínas Virais/genética
14.
Dermatol Online J ; 22(3)2016 Mar 16.
Artigo em Inglês | MEDLINE | ID: mdl-27136625

RESUMO

BACKGROUND: Molluscum contagiosum is a benign viral infection of the skin. Lesions typically present as dome-shaped, flesh-colored, umbilicated papules that range in size from 1 to 5 millimeters in diameter. They are usually asymptomatic, but can become tender or pruritic. Children and immunocompromised adults, including individuals being treated with immunosuppressive drugs, are most susceptible to infection. Single or multiple lesions most commonly appear on the extremities, face, genitals, and trunk. However, albeit rarely, molluscum contagiosum may also develop at other sites, including the eyelids. PURPOSE: We describe the clinical and pathologic findings of a man who developed molluscum contagiosum of the eyelid while receiving methotrexate. We also review the characteristics of other patients with molluscum contagiosum acquired either during treatment with methotrexate or associated with human immunodeficiency virus (HIV) infection and summarize the unusual sites of presentation for the viral lesions in these individuals. MATERIALS AND METHODS: The features of a man receiving methotrexate who developed molluscum contagiosum of the eyelid are presented. Using PubMed, the following terms were searched and relevant citations assessed: adalimumab, contagiosum, Enbrel, etanercept, Humira, infliximab, methotrexate, molluscum, Remicade, TNF alpha, and tumor necrosis factor alpha. In addition, the literature on methotrexate treatment and molluscum contagiosum is reviewed. RESULTS:  Several small papules were observed on the eyelid of a 24-year-old man who had been receiving methotrexate and adalimumab (Humira) for the treatment of Crohn disease. The lesions were removed by shave biopsy. Microscopic examination revealed epidermal hyperplasia composed of keratinocytes filled with large eosinophilic intracytoplasmic inclusions. Based on correlation of the clinical presentation and histopathologic findings, a diagnosis of molluscum contagiosum was established. The patient applied mupirocin 2% ointment to the biopsy sites, which subsequently healed without complication or recurrence. CONCLUSION: Molluscum contagiosum is a benign viral papular eruption that frequently affects children and immunocompromised adults. Patients treated with immunosuppressive agents, such as methotrexate, have a heightened risk of developing molluscum contagiosum lesions. It remains to be determined whether adjunct therapy with a tumor necrosis factor alpha inhibitor increasesthe risk of this viral infection. Diagnosis can usually be established by clinical presentation, although a biopsy is sometimesrequired to exclude other conditions. Molluscum contagiosum is generally self-limiting and often resolves spontaneously within18 months. However, topical (cantharidin) or locally destructive (curettage, cryotherapy, and/or laser) therapy may be indic tedfor patients who are concerned about persistent lesions and for children who are particularly susceptible to autoinoculation.


Assuntos
Doença de Crohn/tratamento farmacológico , Doenças Palpebrais/etiologia , Hospedeiro Imunocomprometido , Imunossupressores/efeitos adversos , Metotrexato/efeitos adversos , Molusco Contagioso/etiologia , Doenças Palpebrais/imunologia , Doenças Palpebrais/patologia , Infecções por HIV/imunologia , Humanos , Masculino , Molusco Contagioso/imunologia , Molusco Contagioso/patologia , Pele/patologia , Adulto Jovem
15.
Mult Scler ; 22(7): 969-71, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-26860987

RESUMO

Fingolimod-related viral infections have been described on several occasions since its introduction in 2010. We hereby add a report on an otherwise immunocompetent, 18-year old Caucasian man with relapsing-remitting multiple sclerosis who developed a protracted and extensive molluscum contagiosum (MC) virus infection shortly after being started on fingolimod. Wide-spread cutaneous MC infections in adult patients are considered indicative of underlying immunosuppression. Neurologists prescribing fingolimod ought to be aware of a possibly increased risk of MC, but also need to know about its relative benignity, lack of extra-cutaneous complications, and adequate treatment options.


Assuntos
Cloridrato de Fingolimode/efeitos adversos , Hospedeiro Imunocomprometido , Imunossupressores/efeitos adversos , Molusco Contagioso/induzido quimicamente , Vírus do Molusco Contagioso/imunologia , Esclerose Múltipla Recidivante-Remitente/tratamento farmacológico , Infecções Oportunistas/induzido quimicamente , Adolescente , Biópsia , Humanos , Masculino , Molusco Contagioso/diagnóstico , Molusco Contagioso/imunologia , Molusco Contagioso/virologia , Esclerose Múltipla Recidivante-Remitente/diagnóstico , Esclerose Múltipla Recidivante-Remitente/imunologia , Infecções Oportunistas/diagnóstico , Infecções Oportunistas/imunologia , Infecções Oportunistas/virologia , Fatores de Risco
16.
Pediatr Dermatol ; 32(4): e193, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25962415

RESUMO

We present a brief observational report of clearance of significant molluscum contagiosum infection in a child taking methotrexate after one dose of intramuscular zoster immunoglobulin-VF (human) and suggest that this may indicate a potential new treatment option.


Assuntos
Soros Imunes/farmacologia , Hospedeiro Imunocomprometido , Molusco Contagioso/tratamento farmacológico , Pré-Escolar , Feminino , Humanos , Molusco Contagioso/imunologia
17.
Adv Virus Res ; 92: 201-52, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25701888

RESUMO

Molluscum contagiosum virus (MCV) is the causative agent of molluscum contagiosum (MC), the third most common viral skin infection in children, and one of the five most prevalent skin diseases worldwide. No FDA-approved treatments, vaccines, or commercially available rapid diagnostics for MCV are available. This review discusses several aspects of this medically important virus including: physical properties of MCV, MCV pathogenesis, MCV replication, and immune responses to MCV infection. Sequencing of the MCV genome revealed novel immune evasion molecules which are highlighted here. Special attention is given to the MCV MC159 and MC160 proteins. These proteins are FLIPs with homologs in gamma herpesviruses and in the cell. They are of great interest because each protein regulates apoptosis, NF-κB, and IRF3. However, the mechanism that each protein uses to impart its effects is different. It is important to elucidate how MCV inhibits immune responses; this knowledge contributes to our understanding of viral pathogenesis and also provides new insights into how the immune system neutralizes virus infections.


Assuntos
Evasão da Resposta Imune , Molusco Contagioso/imunologia , Vírus do Molusco Contagioso/imunologia , Animais , Interações Hospedeiro-Patógeno , Humanos , Molusco Contagioso/virologia , Vírus do Molusco Contagioso/genética , Proteínas Virais/genética , Proteínas Virais/imunologia
18.
Arch Dermatol Res ; 307(3): 275-80, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25384437

RESUMO

Inflammation in atopic dermatitis is mediated in part by the chemokine CCL1 and its receptor, CCR8. Recombinant Molluscum contagiosum viral protein (rMC148p), a cc-chemokine homolog, inhibits CCL1-induced chemotaxis of cells expressing CCR8. rMC148p was prepared using the baculovirus/Sf9 insect cell expression system. The recombinant MC148 fusion protein (rMC148fp), rMC148-TAT-6xHis, was similarly prepared by adding base sequences onto the PCR primers to fuse TAT and 6xHis to rMC148p at the carboxyl terminus. rMC148fp retains the capacity of rMC148p to inhibit CCL1-induced chemotaxis. Furthermore, unlike rMC148p, topically applied rMC148fp penetrates stratum corneum of human neonatal foreskins and concentrates along the basal and lower spinous cell layers of the epidermis. rMC148fp may be a safe and effective agent in the treatment of atopic dermatitis and other CCR8-mediated disorders.


Assuntos
Quimiocina CCL1/metabolismo , Quimiocinas CC/administração & dosagem , Dermatite Atópica/terapia , Epiderme/efeitos dos fármacos , Imunoterapia , Receptores CCR8/metabolismo , Proteínas Recombinantes de Fusão/administração & dosagem , Administração Tópica , Animais , Baculoviridae/genética , Quimiotaxia/efeitos dos fármacos , Protocolos Clínicos , Clonagem Molecular , Dermatite Atópica/imunologia , Epiderme/imunologia , Prepúcio do Pênis/citologia , Humanos , Recém-Nascido , Masculino , Molusco Contagioso/genética , Molusco Contagioso/imunologia , Proteínas Recombinantes de Fusão/genética , Células Sf9 , Spodoptera
19.
J Dermatol ; 41(12): 1058-64, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25438641

RESUMO

Molluscum contagiosum (MC) may persist for many weeks, evading host immunity. We studied the mechanism of immune escape phenomenon in MC, and the possible inducer of apoptosis. Using tissue samples of MC, we examined the numbers of epidermal Langerhans cells (LC), the expression levels of macrophage inflammatory protein-3α (MIP-3α) and thymic stromal lymphopoietin (TSLP), and the apoptotic signals. After molluscum contagiosum virus (MCV) genotyping, we studied the expression of MCV-encoded MC148 mRNA and MC159 mRNA which correspond to viral antagonist for CCR8 and viral Fas-linked interleukin (IL)-1ß converting enzyme (FLICE)-like inhibitor protein (vFLIP), respectively. The nutlin-3-induced apoptosis in MC was observed ex vivo. The numbers of CD1a(+) or Langerin(+) epidermal LC and the expression levels of MIP-3α were markedly decreased in MC. The expression of TSLP was enhanced in the lesional epidermis of atopic dermatitis and human papillomavirus-induced warts, whereas the expression was observed locally in MC. All 14 MC samples examined harbored MCV type 1. The MC148 mRNA was detected in all 14 samples and the MC159 mRNA was detected in 13 samples. Apoptotic cells were absent or at a background level in the living layers of MC, but their numbers were increased in the molluscum bodies by overnight incubation with 5 µmol/L nutlin-3 in culture medium. In conclusion, molluscum bodies are protected from host immune responses and apoptotic signals by being surrounded by LC-depleted epidermal walls and viral immunosuppressive molecules, but could be eradicated by reagents inducing p53-dependent apoptosis.


Assuntos
Apoptose , Molusco Contagioso/imunologia , Quimiocina CCL20/metabolismo , Citocinas/metabolismo , Humanos , Imidazóis , Células de Langerhans , Molusco Contagioso/metabolismo , Molusco Contagioso/patologia , Piperazinas , Verrugas/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA