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1.
Nat Ecol Evol ; 4(3): 288-289, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32127684
2.
Geneva; World Health Organization; 2020-03-10.
em Inglês | WHO IRIS | ID: who-331450
3.
Parasitol Int ; 74: 101961, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31437553

RESUMO

Natural resources are recognized as important sources of potential drugs for treating various infections, and microorganisms are a rich natural source of diverse compounds. Among the world's microorganisms, actinomycetes, which are abundant in soil and marine, are the well-known producers of a wide range of bioactive secondary metabolites and antibiotics. In the present study, four actinomycetes (samples N25, N6, N18, and N12) were isolated from soil samples in Mongolia. Phylogenetic analysis of these isolates revealed that they share the highest similarity with Streptomyces canus (N25), S. cirratus (N6), S. bacillaris (N18) and S. peucetius (N12), based on 16S rRNA gene sequencing. Crude extracts were obtained from them using ethyl acetate, and the crude fractions were separated by thin layer chromatography. The fractions were then evaluated for their cytotoxicities and their anti-Toxoplasma and antimalarial activities in vitro. The S. canus (N25) crude extract was selected for further chemical characterization based on its antiprotozoal activities. Using liquid chromatography-high resolution mass spectrometry, phenazine-1-carboxylic acid (PCA) was detected and identified in the active fractions of the metabolites from strain N25. We next confirmed that commercially available PCA possesses antiprotozoal activity against T. gondii (IC50: 55.5 µg/ml) and Plasmodium falciparum (IC50: 6.4 µg/ml) in vitro. The results of this study reveal that soil actinomycetes are potential sources of antiprotozoal compounds, and that PCA merits further investigation as an anti-protozoal agent.


Assuntos
Antiprotozoários/química , Antiprotozoários/isolamento & purificação , Microbiologia do Solo , Streptomyces/química , Streptomyces/classificação , Mongólia , Filogenia , RNA Ribossômico 16S/genética , Streptomyces/isolamento & purificação
4.
Parasitol Int ; 74: 101996, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31634631

RESUMO

Toxoplasmosis is caused by the protozoan parasite Toxoplasma gondii. Consumption of raw or undercooked meat is the main risk factor for acquiring T. gondii infection in humans. Meat and meat products derived from goats and sheep are mainly consumed in Mongolia; however, there is limited epidemiological information on T. gondii infection in small ruminants in this country. The main objective of the present study was to investigate the seroprevalence of T. gondii in sheep and goats in Mongolia. The seroprevalence of T. gondii IgG antibodies was determined by an indirect enzyme-linked immunosorbent assay based on the recombinant antigens of dense granule protein 7 of T. gondii. A total of 1078 goat and 882 sheep blood samples were collected from 17 of 21 provinces and the capital city of Mongolia. Overall, the seroprevalence of T. gondii among the goat and sheep samples was 32% and 34.8%, respectively. The seroprevalence among goat samples was significantly higher in western (42.7%) and eastern (45.6%) regions compared with other regions (24%). Additionally, the seroprevalence among sheep was significantly higher in eastern regions (55.4%) compared with other regions (26%-33%). Age, but not sex, was considered a risk factor for T. gondii seropositivity in goats, whereas no statistically significant differences were observed in sheep for age or sex. In conclusion, the present study demonstrates the high seroprevalence of T. gondii in small ruminants in Mongolia. Our results highlight that country-wide control measures are required to minimize infections in livestock.


Assuntos
Anticorpos Antiprotozoários/sangue , Cabras/parasitologia , Ovinos/parasitologia , Toxoplasmose Animal/epidemiologia , Fatores Etários , Animais , Feminino , Geografia , Doenças das Cabras/epidemiologia , Doenças das Cabras/parasitologia , Gado/imunologia , Gado/parasitologia , Masculino , Mongólia/epidemiologia , Fatores de Risco , Estudos Soroepidemiológicos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/parasitologia , Toxoplasma , Toxoplasmose Animal/imunologia
5.
J Headache Pain ; 20(1): 114, 2019 Dec 16.
Artigo em Inglês | MEDLINE | ID: mdl-31842733

RESUMO

BACKGROUND: In the ongoing Global Campaign endeavour to improve knowledge and awareness of headache prevalence worldwide, Mongolia is a country of interest. It sits between Russia and China, in which prevalence is, respectively, much higher and much lower than the estimated global mean. We conducted a population-based study in Mongolia both to add to knowledge and to inform local health policy. METHODS: Using standardized methodology with cluster random sampling, we selected Mongolian adults (aged 18-65 years) from five regions reflecting the country's diversities. They were interviewed by trained researchers, cold-calling at their homes, using the Headache-Attributed Restriction, Disability, Social Handicap and Impaired Participation (HARDSHIP) structured questionnaire following pilot-testing. ICHD-3 beta diagnostic criteria were applied. RESULTS: N = 2043 (mean age 38.0 [±13.4] years, 40% urban-dwelling and 60% rural), with a non-participation proportion of 1.7%. Males were somewhat underrepresented, for which corrections were made. The crude 1-year prevalence of any headache was 66.1% (95% CI: 64.0-68.2%), with a strong female preponderance (OR: 2.2; p < 0.0001). Age- and gender-adjusted prevalences were: migraine 23.1% (for females, OR = 2.2; p < 0.0001); tension-type headache (TTH) 29.1% (no gender difference); probable medication-overuse headache (pMOH) 5.7% (trending towards higher in females); other headache on ≥15 days/month 5.0% (for females, OR = 2.2; p = 0.0008). Unclassified cases were only 35 (1.7%). Any headache yesterday was reported by 410 (20.1%; for females, OR = 2.4; p < 0.0001). Only pMOH showed a strong association with age, peaking in middle years with a 5-fold increase in prevalence. Migraine showed a consistent association with educational level, while pMOH showed the reverse, and was also more common among other groups than among participants who were single (never married). Migraine was less common among rural participants than urban (OR: 0.80; p = 0.0326), while pMOH again showed the reverse (OR: 2.4; p < 0.0001). Finally, pMOH (but not migraine or TTH) was significantly associated with obesity (OR: 1.8; p = 0.0214). CONCLUSION: Headache disorders are common in Mongolia, with, most notably, a very high prevalence of headache on ≥15 days/month corroborated by the high prevalence of headache yesterday. The picture is very like that in Russia, and dissimilar to China. There are messages for national health policy.


Assuntos
Transtornos da Cefaleia Primários/diagnóstico , Transtornos da Cefaleia Primários/epidemiologia , Vigilância da População , População Rural , População Urbana , Adolescente , Adulto , Idoso , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mongólia/epidemiologia , Projetos Piloto , Vigilância da População/métodos , Prevalência , Distribuição Aleatória , Inquéritos e Questionários , Adulto Jovem
6.
Artigo em Japonês | MEDLINE | ID: mdl-31875632

RESUMO

Asian dust is a phenomenon involving the long-range transport of atmospheric pollutants originating from the desert areas of China and Mongolia. In recent years, the health effects of Asian dust have raised public concerns. Numerous studies on the health effects of Asian dust have been published since the last review in 2010. Thus, a literature review was conducted to shed light on the latest epidemiologic findings. PubMed and Science Direct databases were used for the review of epidemiologic studies published between June 2009 and April 2018. We identified 53 epidemiologic studies. Mortality, ambulance transportation, hospitalization/medical examination, changes in symptomatic, functional, and examination findings, as well as birth outcomes have been reported as outcomes. When the outcomes were categorized by disease, the effects of Asian dust on respiratory, cardiovascular, and allergic diseases raised concerns. The common evidences of causation between Asian dust and these diseases were the consistency of findings and temporal sequence of association. As results of research on dose-response relationships have become available, and the possibility that the health effects of Asian dust may vary depending on its chemical composition has been pointed out, further research using the exposure level indicators of Asian dust or its chemical composition should be conducted. Furthermore, with focus on the crucial issue of reducing exposure, research related to prevention and raising awareness should be further promoted.


Assuntos
Poluentes Atmosféricos/efeitos adversos , Doenças Cardiovasculares/epidemiologia , Doenças Cardiovasculares/etiologia , Poeira , Exposição Ambiental/efeitos adversos , Hipersensibilidade/epidemiologia , Hipersensibilidade/etiologia , Doenças Respiratórias/epidemiologia , Doenças Respiratórias/etiologia , Areia , Poluentes Atmosféricos/análise , Poluentes Atmosféricos/química , China , Exposição Ambiental/análise , Exposição Ambiental/prevenção & controle , Humanos , Mongólia , Fatores de Tempo
7.
Zootaxa ; 4567(3): zootaxa.4567.3.11, 2019 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-31715891

RESUMO

Following examination of type material of Pherbellia shatalkini Rozkosný, two specimens of snail-killing flies (Sciomyzidae) from Mongolia that initially had been determined as an undescribed species were confirmed as P. shatalkini, the first documentation of this species from Mongolia. The male genitalia are illustrated and redescribed in great detail.


Assuntos
Dípteros , Animais , Genitália Masculina , Masculino , Mongólia
8.
Zootaxa ; 4671(2): zootaxa.4671.2.11, 2019 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-31716062

RESUMO

Barsbold (1981) erected Ingenia yanshini Barsbold, 1981 for a nearly complete oviraptorid skeleton from the Upper Cretaceous (Campanian-Maastrichtian) Barun Goyot Formation in Ömnögov, southern Mongolia. Moreover, he erected a subfamily-level clade Ingeniinae Barsbold, 1981 to distinguish Ingenia yanshini from other oviraptorids such as Oviraptor philoceratops Osborn, 1924 (Barsbold, 1981). When Barsbold first erected the subfamily, he considered this subfamily as monotypic (Barsbold, 1981) but now this subfamily comprisesmultiple taxa such as Machairasaurus leptonychus Longrich, Currie, and Dong, 2010 and Heyuannia huangi Lü, 2002.


Assuntos
Dinossauros , Animais , Fósseis , Mongólia , Crânio
9.
Zootaxa ; 4664(3): zootaxa.4664.3.3, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31716665

RESUMO

Two new species of Casinaria Holmgren, C. camura sp. nov. and C. scalaris sp. nov. and the first record of C. kriechbaumeri (Costa) are recorded from Mongolia. Venturia aquila sp. nov. is described from Ghana. The previously unknown female of V. crassicaput (Morley), an Afrotropical species, is described here for the first time. First records of Venturia anatolica Horstmann from Romania and V. canescens (Gravenhorst) from Mongolia are reported, and an identification key to the Western Palaearctic species of Venturia Schrottky is provided.


Assuntos
Ascomicetos , Himenópteros , Animais , Feminino , Gana , Mongólia , Romênia
10.
Zootaxa ; 4658(2): zootaxa.4658.2.6, 2019 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-31716746

RESUMO

The genus Daphnia O.F. Müller (Crustacea: Cladocera: Daphniidae) has a long history of its study, it attracts maximum attention of evolutionary biologists as compared to other cladoceran taxa. But still there are many unresolved problems concerning the genus taxonomy. We redescribe forgotten Daphnia (D.) turbinata Sars, 1903 which has a relatively narrow distribution in the Altay-Sayan mountain region (southern Siberia and Mongolia). Diagnostic characters of the taxon are discussed as well as possibilities of morphological discrimination of taxa within the D. (D.) longispina species group.


Assuntos
Cladóceros , Animais , Evolução Biológica , Daphnia , Mongólia , Sibéria
11.
Zootaxa ; 4603(2): zootaxa.4603.2.7, 2019 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-31717231

RESUMO

Four new genera and five new species of anaxyelids are described from the Cretaceous of Asia: Mangus magnus gen. et sp. nov. from the Aptian of Mongolia (Bon-Tsagan), Urosyntexis undosa sp. nov., Parasyntexis khasurtensis gen. et sp. nov., both from the Early Cretaceous of Transbaikalia (Khasurty), Dolichosyntexis transbaikalicus gen. et sp. nov. from the Hauterivian-Barremian of Transbaikalia (Baissa), and Curiosyntexis magadanicus gen. et sp. nov. from the mid-Upper Cretaceous of NE Siberia (Obeshchayushchiy). Early Cretaceous is the time of the highest diversity of Anaxyelidae. In this epoch these sawflies are represented with all four subfamilies comprising the group. In Late Cretaceous anaxyelid diversity and abundance rapidly decrease and they are represented with the only subfamily Syntexinae. The anaxyelid collection of Palaeontological Institute (Moscow), the most representative collection of fossil anaxyelids in the world, has been completely revised, identified and described. The list of identifications is provided.


Assuntos
Himenópteros , Animais , Ásia , Fósseis , Mongólia , Moscou , Sibéria
12.
Environ Health Prev Med ; 24(1): 66, 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31775603

RESUMO

BACKGROUND: Children in Ulaanbaatar are exposed to air pollution, but few epidemiological studies have been conducted on the effects of environmental risk factors on children's health. Also, no studies have yet examined the prevalence of respiratory symptoms in children in suburban areas, where air quality-monitoring stations have not yet been installed. This cross-sectional study evaluated the associations between outdoor air pollution and respiratory symptoms among schoolchildren in urban and suburban districts of Ulaanbaatar. METHODS: The ATS-DLD-78 C questionnaire was used to investigate the respiratory symptoms of schoolchildren aged 6-12 years (n = 1190) who lived in one of three urban districts or a suburban district of Ulaanbaatar. In each district, the outdoor concentrations of nitrogen dioxide (NO2) and sulfur dioxide (SO2) were measured at two sites (at ≤100 m and > 100 m from the nearest major road) in the 2-year period from 2015 to 2016. The associations between health outcomes and exposure to air pollutants were estimated using the multinomial logistic regression method. RESULTS: The outdoor concentration of SO2 was significantly associated with persistent cough symptom (OR = 1.12, 95% CI 1.04-1.22). Furthermore, the outdoor concentration of NO2 was significantly associated with the current wheezing symptom (OR = 1.33, 95% CI 1.01-1.75) among children in urban and suburban. CONCLUSIONS: The prevalence of persistent cough symptom was markedly high among the schoolchildren in urban/suburban districts of Ulaanbaatar. Overall, the increases in the prevalence of respiratory symptoms among children might be associated with ambient air pollution in Ulaanbaatar.


Assuntos
Poluentes Atmosféricos/efeitos adversos , Exposição Ambiental/efeitos adversos , Dióxido de Nitrogênio/efeitos adversos , Transtornos Respiratórios/etiologia , Dióxido de Enxofre/efeitos adversos , Poluentes Atmosféricos/análise , Criança , Carvão Mineral/análise , Estudos Transversais , Exposição Ambiental/análise , Monitoramento Ambiental , Feminino , Humanos , Masculino , Mongólia/epidemiologia , Dióxido de Nitrogênio/análise , Prevalência , Transtornos Respiratórios/epidemiologia , Dióxido de Enxofre/análise , Emissões de Veículos/análise
13.
Zootaxa ; 4695(1): zootaxa.4695.1.3, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31719364

RESUMO

Plebejus chrisreai sp. n. and P. anikini azhbogdo ssp. n. are described from the Dzhungarian Gobi desert and the Transaltajan Gobi desert respectively. External distinctions as well as distinctions of the male genitalia are given for the new taxa and two related species known from Mongolia: Plebejus anikini Yakovlev, 2012 and P. germani Yakovlev, 2012. Genitalia of all the taxa mentioned are illustrated, the aedeagus and valva bear the most valuable taxonomic characters. Females of the new species have an unusual whitish colour of the upperside not known for the christophi species complex before. The distribution of Mongolian taxa and relatives from East Kazakhstan is discussed.


Assuntos
Borboletas , Animais , Meio Ambiente , Feminino , Genitália , Cazaquistão , Masculino , Mongólia
14.
Zootaxa ; 4688(2): zootaxa.4688.2.2, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31719447

RESUMO

Three new species of Parasyrisca Schenkel, 1963 belonging to the potanini-group are described from Western Mongolia: P. platnicki sp. n., P. polchaninovae sp. n. and P. szinetari sp. n. Each species description is based on both sexes. Both the male and female of P. platnicki sp. n. possess some copulatory organ characters unknown in other species of the genus. The previously unknown female and the poorly known male of P. khubsugul Ovtsharenko, Platnick et Marusik, 1995 are described and redescribed respectively, the descriptions being based on the specimens found in Arkhangai and Bayankhongor Aimags. The structures of copulatory organs in Parasyrsca potanini-group are briefly discussed.


Assuntos
Aranhas , Distribuição Animal , Estruturas Animais , Animais , Tamanho Corporal , Feminino , Masculino , Mongólia , Tamanho do Órgão
15.
Environ Sci Pollut Res Int ; 26(31): 32420-32431, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31612414

RESUMO

Mongolia has been a pristine environment without much pollution. Our objective is to study a section of the Tuul River to evaluate the present condition of this pristine environment. Sediment metal (Al, Fe, Mn, Cu, Zn, Pb, Ni, Cd, Hg, and Cr) concentrations and Pb-210 were sampled and analyzed. Results showed that metal concentrations are much higher at areas near the capital city and municipal sewage outlet, with enrichment factor values up to 18 for Cu, and 26 for Cr. Higher copper concentrations were found at sites about ~ 50 km downstream from the source, an indication that pollutions are spreading further down the river. Vertical metal concentration profiles indicated that pollutions could be traced back to the 1960s. Inefficient sewage treatment plants and poorly managed power plant ash ponds were major sources of metals leaking into the Tuul River. Sewage wastewater is carrying metals through Tuul River to the lower river basin. Dusts from ash ponds are airborne and transport to greater area. These findings indicate that new and alternative measures have to be enforced to prevent further pollution entering the Tuul River drainage basin and airborne dust to other broader regions of the Asia and ocean.


Assuntos
Sedimentos Geológicos/química , Radioisótopos de Chumbo/análise , Mercúrio/análise , Metais Pesados/análise , Poluentes Químicos da Água/análise , Ásia , Poluição Ambiental , Metais Pesados/química , Mongólia , Rios , Esgotos , Poluentes Químicos da Água/química
16.
Biol Pharm Bull ; 42(10): 1713-1719, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31582659

RESUMO

Asian dust events are caused by dust storms originating from deserts in Mongolia and northern China, and these events are observed in Japan, mainly in spring. To explore the effect of Asian dust events on atmospheric endotoxin and protein levels, we collected the total suspended particles (TSP) in the spring months (March, April, and May) of 2015 in Sasebo and Kyoto, Japan, and assessed the levels of biological elements at both locations. At both locations, the daily concentrations of TSP, water-soluble Ca2+ (an indicator mineral of soil in dust), endotoxins, and proteins were found to be high during and after Asian dust events recorded by the Japan Meteorological Agency. The concentration of Ca2+ showed a strong positive correlation with the concentrations of TSP and endotoxin, while the concentration of protein was moderately positively correlated with Ca2+ in both Sasebo and Kyoto. There were large concentrations of endotoxins, and the fluctuation ranges were higher in Sasebo than in Kyoto. In contrast, protein concentrations showed low levels of fluctuation, and no major differences were found in the concentration at each location.


Assuntos
Poluentes Atmosféricos/análise , Poeira/análise , Endotoxinas/análise , Proteínas/análise , Cálcio/análise , China , Monitoramento Ambiental , Japão , Mongólia , Estações do Ano , Vento
17.
Zhongguo Zhong Yao Za Zhi ; 44(15): 3162-3169, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31602868

RESUMO

This paper investigates and counts the ethnic medicines of the Ewenki,Daur and Oroqen ethnic groups,which are known as the " Three Minorities" in Inner Mongolia. Through the methods of literature collection,interview investigation,and resource investigation,different ethnic medicines were collected on the main diseases,drug varieties,drug-injection sites,and drug administration methods. Through data statistics and SPSS analysis,the similarities and individual differences between the three ethnic groups were clarified. The results indicated the predicament of the current national medicine,which is helpful for the protection and inheritance of ethnic medicine.


Assuntos
Grupos Étnicos , Etnofarmacologia , China , Humanos , Mongólia
18.
Environ Sci Pollut Res Int ; 26(32): 33494-33506, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31529344

RESUMO

Unegt subbasin in Dornogobi Province, southeastern Mongolia, contains the Dulaan Uul uranium deposit, for which development for commercial mining has been conducted as of 2015. Zuunbayan is a commune located close to the Dulaan Uul uranium deposit, and residents of Zuunbayan and their livestock can easily approach the uranium deposit area, including an aboveground dump site, which was created as a result of the mining development. The present study measured and analyzed the gamma dose rate (absorbed dose rate in air) distribution in Unegt subbasin using data collected from a car-borne measurement survey. The gamma dose rate increased from the northern (45-65 nGy/h) to the central (50-69 nGy/h, including Zuunbayan) and the southern (54-195 nGy/h, including Dulaan Uul) parts of the study area. The gamma dose rates (up to 195 nGy/h) around the dump site in Dulaan Uul were significantly higher than the background level (< 80 nGy/h) at several points. Additional in-situ measurements showed that the gamma dose rates reached up to 450 nGy/h at these locations, which was primarily attributed to the gamma radiation emitted by 238U series elements. Spatial distribution of gamma dose rates around the dump site revealed that the gamma radiation did not originate from the dump, but from the ground, at the measurement points. Analysis of collected soil samples showed that 238U and 226Ra were concentrated in deeper soil. These results indicate that the gamma dose rates higher than the background level were not associated with the aboveground mine dump; rather, they were very probably caused by presence of uranium deposits close to the ground surface.


Assuntos
Radiação de Fundo , Raios gama , Monitoramento de Radiação , Poluentes Radioativos do Solo/análise , Mineração , Mongólia , Radioisótopos de Potássio/análise , Doses de Radiação , Solo , Tório/análise , Urânio/análise
19.
J Biosci ; 44(4)2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31502575

RESUMO

The parasitic nematode Haemonchus contortus is one of the world's most important parasites of small ruminants that causes significant economic losses to the livestock sector. The population structure and selection in its various strains are poorly understood. No study so far compared its different populations using genome-wide data. Here, we focused on different geographic populations of H. contours from China (Tibet, TB; Hubei, HB; Inner Mongolia, IM; Sichuan, SC), UK and Australia (AS), using genome-wide population-genomic approaches, to explore genetic diversity, population structure and selection. We first performed next-generation high-throughput 2b RAD pool sequencing using Illumina technology, and identified single-nucleotide polymorphisms (SNPs) in all the strains. We identified 75,187 SNPs for TB, 82,271 for HB, 82,420 for IM, 79,803 for SC, 83,504 for AS and 78,747 for UK strain. The SNPs revealed low-nucleotide diversity (pi= 0.0092-0.0133) within each strain, and a significant differentiation level (average Fst = 0.34264) among them. Chinese populations TB and SC, along with the UK strain, were more divergent populations. Chinese populations IM and HB showed affinities to the Australian strain. We then analysed signature of selection and detected 44 (UK) and 03 (AS) private selective sweeps containing 49 and 05 genes, respectively. Finally, we performed the functional annotation of selective sweeps and proposed biological significance to signature of selection. Our data suggest that 2b-RAD pool sequencing can be used to assess the signature of selection in H. contortus.


Assuntos
Resistência a Medicamentos/genética , Haemonchus/genética , Doenças Parasitárias/genética , Animais , Austrália/epidemiologia , China/epidemiologia , Variação Genética , Genótipo , Haemonchus/patogenicidade , Sequenciamento de Nucleotídeos em Larga Escala , Mongólia/epidemiologia , Doenças Parasitárias/epidemiologia , Doenças Parasitárias/parasitologia , Polimorfismo de Nucleotídeo Único/genética , Tibet/epidemiologia , Reino Unido/epidemiologia
20.
J Toxicol Sci ; 44(9): 633-641, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31474744

RESUMO

Asian Sand Dust-Particulate Matter (ASD-PM) aerosol brings large amounts of wind-eroded soil particles containing high concentrations of metallic components caused by industrialization and vehicles. Proinflammatory and cytotoxic cytokines trigger local inflammatory responses and cause a systematically high incidence of cardiovascular and other diseases. Tenascin C (Tn-C) is known to be expressed in damaged tissue or in a developmental stage of tissue. In this study, we examined the expression of Tn-C and Fibronectin in human cancer-cell lines and in liver tissue of mice treated with ASD-PM to investigate the inflammatory and cell-damage effects of ASD-PM. In our in vivo study, mice were intratracheally instilled with saline suspensions of ASD-PM particles. Instillation of these particles was repeated twice a week for 12 weeks and the liver tissues were stained with hematoxylin, eosin, and Masson's trichrome, and we carried out an IF. Tn-C expression in liver tissues was detected by RT-PCR and western blot analysis. In the results, the expression of Tn-C increased in a dose-dependent manner in both RNA and Immunofluorescence assay (IF). In our in vitro study, A549 and Hep3B cell lines were incubated in culture media with Transforming Growth Factor-Beta1(TGF-ß1) and ASD-PM. Immunofluorescence microscopy images showed a two times stronger expression of fluorescence in the ASD-treated group than in that treated with TGF-ß1. They also showed a stronger expression of Tn-C in proportion to the concentration of ASD-PM. We confirmed that ASD-PM when inhaled formally migrated to other organs and induced Tn-C expression. ASD-PM containing metals causes expression of Tn-C in liver tissue in proportion to the concentration of ASD-PM.


Assuntos
Carcinoma Hepatocelular/metabolismo , Poeira , Exposição Ambiental/efeitos adversos , Neoplasias Hepáticas/metabolismo , Fígado/metabolismo , Material Particulado/toxicidade , Areia , Tenascina/metabolismo , Aerossóis , Animais , Linhagem Celular Tumoral , China , Fibronectinas/genética , Fibronectinas/metabolismo , Expressão Gênica , Humanos , Fígado/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mongólia , Material Particulado/metabolismo , Tenascina/genética
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