Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.707
Filtrar
1.
Molecules ; 25(17)2020 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-32842606

RESUMO

Presently, there are no approved drugs or vaccines to treat COVID-19, which has spread to over 200 countries and at the time of writing was responsible for over 650,000 deaths worldwide. Recent studies have shown that two human proteases, TMPRSS2 and cathepsin L, play a key role in host cell entry of SARS-CoV-2. Importantly, inhibitors of these proteases were shown to block SARS-CoV-2 infection. Here, we perform virtual screening of 14,011 phytochemicals produced by Indian medicinal plants to identify natural product inhibitors of TMPRSS2 and cathepsin L. AutoDock Vina was used to perform molecular docking of phytochemicals against TMPRSS2 and cathepsin L. Potential phytochemical inhibitors were filtered by comparing their docked binding energies with those of known inhibitors of TMPRSS2 and cathepsin L. Further, the ligand binding site residues and non-covalent interactions between protein and ligand were used as an additional filter to identify phytochemical inhibitors that either bind to or form interactions with residues important for the specificity of the target proteases. This led to the identification of 96 inhibitors of TMPRSS2 and 9 inhibitors of cathepsin L among phytochemicals of Indian medicinal plants. Further, we have performed molecular dynamics (MD) simulations to analyze the stability of the protein-ligand complexes for the three top inhibitors of TMPRSS2 namely, qingdainone, edgeworoside C and adlumidine, and of cathepsin L namely, ararobinol, (+)-oxoturkiyenine and 3α,17α-cinchophylline. Interestingly, several herbal sources of identified phytochemical inhibitors have antiviral or anti-inflammatory use in traditional medicine. Further in vitro and in vivo testing is needed before clinical trials of the promising phytochemical inhibitors identified here.


Assuntos
Antivirais/química , Betacoronavirus/efeitos dos fármacos , Catepsina L/química , Compostos Fitoquímicos/química , Inibidores de Proteases/química , Receptores Virais/química , Serina Endopeptidases/química , Sequência de Aminoácidos , Antivirais/isolamento & purificação , Antivirais/farmacologia , Betacoronavirus/patogenicidade , Sítios de Ligação , Catepsina L/antagonistas & inibidores , Catepsina L/genética , Catepsina L/metabolismo , Infecções por Coronavirus/tratamento farmacológico , Infecções por Coronavirus/enzimologia , Infecções por Coronavirus/virologia , Cumarínicos/química , Cumarínicos/isolamento & purificação , Cumarínicos/farmacologia , Expressão Gênica , Ensaios de Triagem em Larga Escala , Interações Hospedeiro-Patógeno/efeitos dos fármacos , Interações Hospedeiro-Patógeno/genética , Humanos , Índia , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Monossacarídeos/química , Monossacarídeos/isolamento & purificação , Monossacarídeos/farmacologia , Pandemias , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Plantas Medicinais/química , Pneumonia Viral/tratamento farmacológico , Pneumonia Viral/enzimologia , Pneumonia Viral/virologia , Inibidores de Proteases/isolamento & purificação , Inibidores de Proteases/farmacologia , Ligação Proteica , Conformação Proteica em alfa-Hélice , Conformação Proteica em Folha beta , Domínios e Motivos de Interação entre Proteínas , Quinazolinas/química , Quinazolinas/isolamento & purificação , Quinazolinas/farmacologia , Receptores Virais/antagonistas & inibidores , Receptores Virais/genética , Receptores Virais/metabolismo , Serina Endopeptidases/genética , Serina Endopeptidases/metabolismo , Termodinâmica , Internalização do Vírus/efeitos dos fármacos
2.
Chemosphere ; 251: 126368, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32171941

RESUMO

Mangrove sediments act as an important natural sink and a secondary source for trace metals. The main objective of this study was to investigate metal contamination and its relationship to mangrove-derived carbohydrates in mangrove sediments. Sixteen metals (Be, V, Cr, Co, Ni, Cu, Zn, Ga, As, Sr, Cd, Sn, Sb, Ba, Tl, and Pb)were analyzed in the surface sediments from four sites at different latitudes on the southeast coastline of China. The sedimentary organic matter was characterized by Rock-Eval pyrolysis, and the neutral sugars were examined by gas chromatograph mass spectrometry. Our results from the enrichment factors indicated that the mangrove sediments were no enriched by Ga, Sr, and Ba, minor enriched by Be, V, Cr, Co, Ni, Cu, Zn, As, Sn, Sb, Tl, and Pb, and moderate enriched by Cd. Litterfall was a major source of organic matter in the mangrove sediments, and the neutral sugars were mainly derived from this litterfall. Significant correlations were detected between the total organic carbon, pyrolytic parameters, neutral sugars, and enrichment factors of V, Cr, Co, Ni, Zn, and Cd, suggesting the input of neutral carbohydrates played an important role in enhancing the metal accumulation in the mangrove sediments. The mangrove litterfall itself was a major source of metals for the sediments, and the mangrove-derived organic matter enhanced the sediment's metal accumulation.


Assuntos
Monitoramento Ambiental , Metais Pesados/química , Monossacarídeos/química , China , Sedimentos Geológicos/química , Metais Pesados/análise , Oligoelementos/análise , Poluentes Químicos da Água/análise , Áreas Alagadas
3.
Org Biomol Chem ; 18(7): 1349-1353, 2020 02 19.
Artigo em Inglês | MEDLINE | ID: mdl-32037424

RESUMO

Ionic polysaccharides are part of many biological events, but lack structural characterisation due to challenging purifications and complex synthesis. Four monosaccharides bearing modifications not found in nature are used for the automated synthesis of a collection of ionic oligosaccharides. Structural analysis reveals how the charge pattern affects glycan conformation.


Assuntos
Automação , Oligossacarídeos/síntese química , Íons/química , Simulação de Dinâmica Molecular , Estrutura Molecular , Monossacarídeos/química , Oligossacarídeos/química
4.
J Am Soc Mass Spectrom ; 31(2): 249-256, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32031404

RESUMO

Identification and confirmation of known as well as unknown (bio)chemical entities in ambient mass spectrometry (MS) and MS imaging (MSI) mostly involve accurate mass determination, often in combination with MS/MS or MSn work flows. To further improve structural assignment, additional molecular information is required. Here we present an ambient hydrogen/deuterium exchange (HDX) laser ablation electrospray ionization (LAESI) MS method in which, apart from the accurate mass and MS/MS data, the number of exchangeable protons in (un)known molecules is obtained. While eventually presenting ambient HDX-LAESI-MSI, samples were not preincubated with deuterated solvents, but instead HDX occurred following fusion of ablated sample material with microdroplets generated by ESI of deuterated solvents. Therefore, the degree of HDX was first studied following ablation of nondeuterated sample solutions of melamine and monosaccharides. From these experiments, it was concluded that the set-up used could provide meaningful HDX data in support of molecular structure elucidation by significantly reducing the number of structure options from a measured elemental composition. This reduction was demonstrated with an unknown accurate m/z value obtained in the analysis of an orange slice, reducing the possible number of molecular structures having the same elemental composition by 87% due to the number of H/D exchanges observed. Next, deuterated and nondeuterated MS/MS experiments showed the number of exchangeable protons in the substructures from deuterated neutral losses in the product ion spectra, confirming the compound to be arginine. Finally, the potential of ambient HDX-LAESI-MSI was demonstrated by the imaging of (secondary) plant metabolites in a Phalaenopsis petal.


Assuntos
Medição da Troca de Deutério/métodos , Monossacarídeos/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Triazinas/química , Hidrogênio/química , Terapia a Laser , Prótons , Espectrometria de Massas em Tandem/métodos
5.
Carbohydr Polym ; 231: 115688, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31888812

RESUMO

Two new isolated polysaccharides, ARPP-40 (40 % ethanol precipitate) and ARPP-70 (70 % ethanol precipitate), were extracted from Anoectochilus roxburghii. The physicochemical properties of two polysaccharides were analyzed and the results showed the relative weight average molecular weights and contents of neutral sugar for ARPP-40 and ARPP-70 were 423 kDa and 97.4 %, 10.8 kDa and 51.4 %, respectively. In terms of monosaccharide composition, ARPP-40 contained only glucose, while ARPP-70 was composed of seven monosaccharides, of which glucose and galactose were the main components. Furthermore, the structure and conformation characteristics of ARPP-40 were systematically investigated. The results revealed that ARPP-40 was supposed to be a glucan and existed as a flexible chain with a polydispersity index of 1.02 in 0.1 M NaNO3 solution. The systematic information on structural and conformational properties of ARPP-40 was meaningful for its further application in food and medicinal industry.


Assuntos
Orchidaceae/química , Polissacarídeos/química , Açúcares/química , Antioxidantes/química , Fracionamento Químico , Galactose/química , Glucose/química , Peso Molecular , Monossacarídeos/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/ultraestrutura , Açúcares/isolamento & purificação
6.
Carbohydr Polym ; 231: 115728, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31888838

RESUMO

A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var. Wilsonii with a molecular weight of 86.04 kDa. By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of →2,4)-α-Manp-(1→ and →4)-α-GalpA-(1→ (60 % esterified), with an araban branch attached to O-2 of →2,4)-α-Manp-(1→. The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis. XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 ± 18.74 µg/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade. However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50: 268.55 ± 16.82 µg/mL) or the branches were removed by partial hydrolysis (CH50: 197.76 ± 21.81 µg/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.


Assuntos
Proteínas Inativadoras do Complemento/química , Juniperus/química , Polissacarídeos/química , Ácidos/química , Cromatografia Líquida , Complemento C3/antagonistas & inibidores , Complemento C3/química , Proteínas Inativadoras do Complemento/isolamento & purificação , Proteínas Inativadoras do Complemento/farmacologia , Carboidratos da Dieta/farmacologia , Humanos , Hidrólise , Peso Molecular , Monossacarídeos/química , Polissacarídeos/isolamento & purificação , Polissacarídeos/farmacologia , Espectrometria de Massas em Tandem , Ácidos Urônicos/química , Água/química
7.
Soft Matter ; 16(5): 1246-1258, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31912078

RESUMO

Simple sugars such as glucose and sucrose are ubiquitous in all organisms. One remarkable property of these small solutes is their ability to protect biomembranes against dehydration damage. This property, which reflects the underlying sugar-lipid interactions, has been intensely studied for lipid bilayers interacting with a single sugar at low hydration. Here, we use giant vesicles to investigate fully hydrated lipid membranes in contact with two sugars, glucose and sucrose. The vesicles were osmotically balanced, with the same total sugar concentration in the interior and exterior aqueous solutions. However, the two solutions differed in their composition: the interior solution contained only sucrose whereas the exterior one contained primarily glucose. This sugar asymmetry generated a striking variety of multispherical or "multi-balloon" vesicle shapes. Each multisphere involved only a single membrane that formed several spherical segments, which were connected by narrow, hourglass-shaped membrane necks. These morphologies revealed that the sugar-lipid interactions generated a significant spontaneous curvature with a magnitude of about 1 µm-1. Such a spontaneous curvature can be generated both by depletion and by adsorption layers of the sugar molecules arising from effectively repulsive and attractive sugar-lipid interactions. All multispherical shapes are stable over a wide range of parameters, with a substantial overlap between the different stability regimes, reflecting the rugged free energy landscape in shape space. One challenge for future studies is to identify pathways within this landscape that allow us to open and close the membrane necks of these shapes in a controlled and reliable manner. We will then be able to apply these multispheres as metamorphic chambers for chemical reactions and nanoparticle growth.


Assuntos
Membrana Celular/metabolismo , Monossacarídeos/metabolismo , Lipossomas Unilamelares/química , Adsorção , Membrana Celular/química , Glucose/análise , Glucose/metabolismo , Lipídeos/química , Monossacarídeos/química , Osmose , Lipossomas Unilamelares/metabolismo
8.
Carbohydr Polym ; 230: 115593, 2020 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-31887873

RESUMO

The aim of this study was to investigate the in vitro digestion and fermentation prebiotic properties of three released-exopolysaccharide fractions (r-EPS1, r-EPS2 and r-EPS3) from Lactobacillus delbrueckii ssp. bulgaricus SRFM-1. There were no free oligosaccharides and/or monosaccharides for r-EPS1 before and after simulated buccal, gastric and small intestinal (GSI) digestion in vitro. In contrast, r-EPS2 (13.4 %) and r-EPS3 (10.6 %) generated a few monosaccharides after digestion. Additionally, r-EPS1 and r-EPS2 seemed to present a strong bifidogenic effect comparing to inulin, as they exhibited high values of selectivity index (13.17 and 12.84, respectively). Furthermore, the fermentation with r-EPS1 produced the highest contents of acetic acid and lactic acid (56.3 mM and 44.29 mM, respectively), which resulted in the highest amounts of total short chain fatty acid (145.51 mM) followed by r-EPS2 (135.57 mM) and inulin (99.28 mM). These results indicated that r-EPS from L. delbrueckii ssp. bulgaricus SRFM-1 could be a good potential candidate for new functional food prebiotic.


Assuntos
Digestão/efeitos dos fármacos , Lactobacillus delbrueckii/química , Polissacarídeos Bacterianos/química , Polissacarídeos/química , Meios de Cultura/química , Fermentação , Humanos , Ácido Láctico/química , Lactobacillus delbrueckii/metabolismo , Monossacarídeos/química , Polissacarídeos/metabolismo , Polissacarídeos Bacterianos/metabolismo
9.
J Chromatogr A ; 1609: 460459, 2020 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-31445800

RESUMO

Sulfate (SO4-sug) and sulfonate (SO3-sug) arsenosugar standard solutions were obtained using preparative liquid chromatography. Several commercial algae samples were characterized (total contents and speciation) to select the most appropriate in relation to their arsenosugar contents. Water extracts from the selected sample (Fucus vesiculosus) were fractionated using a Hamilton PRP-X100 preparative column, and the presence of arsenic species in the isolated fractions was ascertained by IC-ICP-MS. Two of the fractions successfully presented only one arsenic species corresponding to sulfate and sulfonate arsenosugars at suitable concentrations. To unequivocally confirm the presence of both compounds, high-resolution mass spectrometry (ESI-TOF/MS) was used and the exact mass determined with errors lower than 0.5 ppm. The standard solutions obtained were successfully used to identify and quantify SO4-sug and SO3-sug in several edible algae samples purchased in local market. Total arsenic content for analyzed samples ranged from 34 to 57 mg kg-1, concentration values found for SO3-sug ranged from 5 to 36 mg As kg-1 and SO4-sug was only found in fucus with a concentration of 9.3 mg As kg-1.


Assuntos
Arseniatos/química , Arseniatos/isolamento & purificação , Monossacarídeos/química , Monossacarídeos/isolamento & purificação , Feófitas/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Cromatografia Líquida , Espectrometria de Massas , Água/química
10.
J Nat Med ; 74(1): 170-188, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31578667

RESUMO

Saposhnikoviae Radix (SR) is a commonly used crude drug that is obtained from the root and rhizome of Saposhnikovia divaricata which is distributed throughout China, Korea, Mongolia, and Russia. To evaluate the quality of Mongolian S. divaricata, metabolomic profiling of 43 plant specimens from different regions of Mongolia, as well as 8 SR samples and 2 plant specimens from China, were conducted by liquid chromatography-ion-trap-time-of-flight-mass spectrometer (LC-IT-TOF-MS). LC-MS profiles of the specimens showed uniformity and 30 compounds were tentatively identified, including 13 chromones and 17 coumarins. Among them, 16 compounds were isolated and unambiguously verified by comparing them with the spectroscopic data of standard compounds. Orthogonal partial least squares-discriminant analysis (OPLS-DA) based on LC-MS data from 7 Mongolian specimens and 8 Chinese SR samples as well as 2 plant specimens revealed that these 2 groups were clearly distinguishable and that Mongolian specimens were characterized by an abundance of prim-O-glucosylcimifugin (1). Moreover, the OPLS-DA of the Mongolian specimens showed that they can be discriminated by their growing regions based on the content of 8 chromones. The total content of dihydrofurochromones 1-3 was relatively higher in the specimens from Khalkhgol in the far eastern part of Mongolia, while contents of 10, 11, 15, and 16 were higher in those from Holonbuir in the eastern part. Based on this research, the roots of S. divaricata from Mongolia have potential as a new resource of SR in Kampo medicine.


Assuntos
Apiaceae/química , Cromonas/análise , Cromonas/química , Cumarínicos/química , Monossacarídeos/química , Xantenos/química , China , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Líquida , Medicamentos de Ervas Chinesas/química , Medicina Kampo , Mongólia , Raízes de Plantas/química , Rizoma/química , Espectrometria de Massas em Tandem/métodos
11.
Dev Comp Immunol ; 102: 103485, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31461636

RESUMO

The Fasciola hepatica/Pseudosuccinea columella interaction in Cuba involves a unique pattern of phenotypes; while most snails are susceptible, some field populations are naturally resistant to infection and parasites are encapsulated by snail hemocytes. Thus, we investigated the hemocytes of resistant (R) and susceptible (S) P. columella, in particular morphology, abundance, proliferation and in vitro encapsulation activity following exposure to F. hepatica. Compared to susceptible P. columella, hemocytes from exposed resistant snails showed increased levels of spreading and aggregation (large adherent cells), proliferation of circulating blast-like cells and encapsulation activity of the hemocytes, along with a higher expression of the cytokine granulin. By contrast, there was evidence of a putative F. hepatica-driven inhibition of host immunity, only in susceptible snails. Additionally, (pre-)incubation of naïve hemocytes from P. columella (R and S) with different monosaccharides was associated with lower encapsulation activity of F. hepatica larvae. This suggests the involvement in this host-parasite interaction of lectins and lectins receptors (particularly related to mannose and fucose sensing) in association with hemocyte activation and/or binding to F. hepatica.


Assuntos
Resistência à Doença , Fasciola hepatica/fisiologia , Hemócitos/imunologia , Interações Hospedeiro-Parasita/imunologia , Larva/fisiologia , Caramujos/imunologia , Animais , Diferenciação Celular , Proliferação de Células , Cuba , Suscetibilidade a Doenças , Expressão Gênica , Granulinas/genética , Granulinas/imunologia , Hemócitos/parasitologia , Imunidade Inata , Monossacarídeos/química , Monossacarídeos/imunologia , Fenótipo , Caramujos/parasitologia
12.
Phys Chem Chem Phys ; 21(44): 24269-24285, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31670327

RESUMO

An enormous population worldwide is presently confronted with debilitating neurodegenerative diseases. The etiology of the disease is connected to protein aggregation and the events involved therein. Thus, a complete understanding of an inhibitor at different stages in the process is imperative for the formulation of a drug molecule. This review presents a detailed summary of the current status of different cosolvents. It further develops how the complex aggregation pathway can be simplified into three steps common to all proteins and the way computer simulations can be exploited to gain insights into the ways by which known inhibitors can affect all these stages. Computation of theoretical parameters in this regard and their correlation with experimental techniques is accentuated. In addition to providing an outline of the scope of different additives, this review showcases the way by which the problem of analyzing an effect of an additive can be addressed effectively via MD simulations.


Assuntos
Proteínas/química , Antioxidantes/química , Antioxidantes/farmacologia , Desenho de Fármacos , Humanos , Simulação de Dinâmica Molecular , Monossacarídeos/química , Monossacarídeos/farmacologia , Doenças Neurodegenerativas/metabolismo , Doenças Neurodegenerativas/patologia , Agregados Proteicos/efeitos dos fármacos , Proteínas/metabolismo
13.
Molecules ; 24(20)2019 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-31615093

RESUMO

Microbial infections remains a serious challenge in food industries due to their resistance to some of the well-known antibacterial and antifungal agents. In this work, a novel monomyristoyl ester (fructosyl monomyristate) and two other derivatives (i.e., glucosyl and galactosyl monomyristates) were successfully synthesized from myristic acid and monosaccharides in two-step reactions. First, the myristic acid was converted to myristoyl chloride, and then the myristoyl chloride was reacted with fructose, glucose and galactose separately to produce the corresponding monosaccharide monomyristate derivatives. The structures of the synthesized products were confirmed by Fourier transform infrared (FTIR), proton and carbon nuclear magnetic resonance (1H- and 13C-NMR), and mass spectral (MS) data. The monomyristates esters were obtained in reaction yields of 45.80%-79.49%. The esters were then evaluated for their antimicrobial activity using the disc diffusion test. It was found that the esters exhibited a medium antibacterial activity against gram-positive bacteria; however, they showed a weak antibacterial activity against gram-negative bacteria. Amongst the esters, galactosyl myristate yielded the highest antibacterial activity against Salmonella typhimurium, Staphylococcus aureus and Bacillus subtilis, while glucosyl monomyristate exhibited the highest antibacterial activity only against Escherichia coli. Additionally, all products showed remarkable antifungal activity against Candida albicans. These findings demonstrate that monosaccharide monomyristate derivatives are promising for use as biocompatible antimicrobial agents in the future.


Assuntos
Anti-Infecciosos/química , Antifúngicos/química , Estrutura Molecular , Monossacarídeos/química , Anti-Infecciosos/farmacologia , Antifúngicos/farmacologia , Candida albicans/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Ésteres/química , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade Microbiana , Monossacarídeos/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Relação Estrutura-Atividade
14.
J Biotechnol ; 306: 185-192, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31629784

RESUMO

It has been reported that certain biomass-degrading bacteria can produce bioflocculant through directly utilizing untreated biomass as carbon source. However, little is known about the synthesis mechanism of bioflocculant in these bacteria. In this study, a biomass-degrading bacterium Pseudomonas sp. HP2 showing excellent production ability of bioflocculant was isolated from the forest soil. The HP2 strain secreted alkali-thermo-tolerant CMCase and xylanase, with the maximum activities of 0.06 and 1.07 U ml-1, respectively, when the untreated rice straw was used as carbon source. The maximum flocculating efficiency with the value of 92.5% was produced from untreated rice straw by HP2 strain. Component analysis showed that this bioflocculant was abundant in the amino acids and monosaccharides with the total contents of 384.9 and 478.3 mg g-1 dry bioflocculant, respectively. The most amino acid and monosaccharide in this bioflocculant were proline and rhamnose, which accounted for 26.5% and 33.3% of total amino acids and total monosaccharides, respectively. To explore the synthesis mechanism of bioflocculant in HP2, the genome of HP2 strain was measured by Illumina HiSeq PE150 platform. The results showed that the genome of HP2 strain possessed abundant CAZy family related genes, which may play an important role in biomass degradation and bioflocculant synthesis.


Assuntos
Glicoproteínas/metabolismo , Oryza/metabolismo , Pseudomonas/metabolismo , Aminoácidos/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Biodegradação Ambiental , Biomassa , Carbono/metabolismo , Celulase/genética , Celulase/metabolismo , Endo-1,4-beta-Xilanases/genética , Endo-1,4-beta-Xilanases/metabolismo , Floculação , Genoma Bacteriano/genética , Glicoproteínas/química , Monossacarídeos/química , Pseudomonas/enzimologia , Pseudomonas/genética , Pseudomonas/isolamento & purificação , Microbiologia do Solo
15.
Molecules ; 24(20)2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31623207

RESUMO

Two novel water soluble heteroglycan (PCp-I and PCp-II) with anti-A549 lung cancer cells activity were isolated from Psoralea corylifolia L. Their average molecular weights were 2.721 × 104 and 2.850 × 104. PCp-I and PCp-II had the same monosaccharide composition, but their molar ratios were different. Based on methylation and NMR spectroscopy, the part structure of PCp-I was identified. The results of scanning electron microscope (SEM) showed that PCp-I had an irregular porous structure and PCp-II was flaky and irregularly curved. The results of thermogravimetry-differential scanning calorimetry (TG-DSC) showed that PCp-I and PCp-II had good thermal stability. Furthermore, PCp-I and PCp-II exhibited significant anti-A549 lung cancer cells activity (IC50 = 64.84 and 126.30 µM) in vitro.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Extratos Vegetais/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Psoralea/química , Células A549 , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Fenômenos Químicos , Humanos , Espectroscopia de Ressonância Magnética , Metilação , Peso Molecular , Monossacarídeos/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Polissacarídeos/isolamento & purificação , Espectroscopia de Infravermelho com Transformada de Fourier
16.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3608-3614, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602930

RESUMO

In order to provide scientific basics for exploitation and sufficient application of Polyporus umbellatus resources and study the monosaccharide composition of P. umbellatus polysaccharides,the anthrone-sulfuric acid method was applied to compare polysaccharide content of P. umbellatus from 17 producing areas. The monosaccharides were derived by 1-phenyl-3-methyl-5-pyrazolone( PMP) and the derivatives were identified by UPLC-MS/MS and the content of each monosaccharide component was determined simultaneously. The results demonstrated that there was a certain difference in total polysaccharide content of P. umbellatus from different regions,and the content of total P. umbellatus polysaccharide from Shaanxi province and Sichuan province( 1. 15% and 1. 90%) was relatively higher than that of others areas. Polysaccharides from P. umbellatus was mainly composed of eight monosaccharides,including glucose,glucuronic acid,galactose,ribose,xylose,arabinose,mannose and fucose. The contents of glucose( 17. 65 mg·g-1) was higher than others. The ribose was the lowest( 0. 13 mg·g-1). In addition,fructose,rhamnose and galacturonic acid were also detected in some samples. Furthermore,the results of cluster analysis( CA) and principal component analysis( PCA) indicated that totally 17 batches of P. umbellatus polysaccharide could be classified into three clusters,samples collected from Wuchang in Heilongjiang province were clustered into one group separately. The study can provide a basis for rational utilization of P. umbellatus resources,and also implies the sequence of monosaccharide linking and pharmacological activity of P. umbellatus polysaccharides.


Assuntos
Monossacarídeos/química , Polyporus/química , Polissacarídeos/química , China , Cromatografia Líquida de Alta Pressão , Geografia , Espectrometria de Massas em Tandem
17.
Molecules ; 24(20)2019 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-31600890

RESUMO

In this study, an acidic polysaccharide from Codonopsis pilosula Nannf. var. modesta (Nannf.) L. T. Shen (WCP-I) and its main fragment, WCP-Ia, obtained after pectinase digestion, were structurally elucidated and found to consist of a rhamnogalacturonan I (RG-I) region containing both arabinogalactan type I (AG-I) and type II (AG-II) as sidechains. They both expressed immunomodulating activity against Peyer's patch cells. Endo-1,4-ß-galactanase degradation gave a decrease of interleukine 6 (IL-6) production compared with native WCP-I and WCP-Ia, but exo-α-l-arabinofuranosidase digestion showed no changes in activity. This demonstrated that the stimulation activity partly disappeared with removal of ß-d-(1→4)-galactan chains, proving that the AG-I side chain plays an important role in immunoregulation activity. WCP-Ia had a better promotion effect than WCP-I in vivo, shown through an increased spleen index, higher concentrations of IL-6, transforming growth factor-ß (TGF-ß), and tumor necrosis factor-α (TNF-α) in serum, and a slight increment in the secretory immunoglobulin A (sIgA) and CD4+/CD8+ T lymphocyte ratio. These results suggest that ß-d-(1→4)-galactan-containing chains in WCP-I play an essential role in the expression of immunomodulating activity. Combining all the results in this and previous studies, the intestinal immune system might be the target site of WCP-Ia.


Assuntos
Codonopsis/química , Fatores Imunológicos/farmacologia , Imunomodulação/efeitos dos fármacos , Extratos Vegetais/farmacologia , Polissacarídeos/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Hidrólise , Imunidade nas Mucosas/efeitos dos fármacos , Fatores Imunológicos/química , Camundongos , Estrutura Molecular , Monossacarídeos/química , Nódulos Linfáticos Agregados/efeitos dos fármacos , Nódulos Linfáticos Agregados/imunologia , Nódulos Linfáticos Agregados/metabolismo , Extratos Vegetais/química , Polissacarídeos/química , Análise Espectral
18.
Artigo em Inglês | MEDLINE | ID: mdl-31476578

RESUMO

A baseline dip caused by the reduction of dissolved oxygen in samples has been a source of trouble in the analysis of major monosaccharides (galactose, glucose, mannose, and fructose) in the high-performance anion-exchange chromatography with pulsed amperometric detection system. This study attempted three different methods to remove the baseline dip from the resulting chromatograms, and among the approaches, sodium sulfite was found to act as the best oxygen scavenger. Clean chromatograms were obtained by adding at least 3 mg/mL sodium sulfite to samples, which removed the baseline dip and improved the accuracy of sugar analysis. Although sodium sulfite does not influence analytical sensitivity, it can cause a reduction of sugar retention; however, retention time can be recovered by washing with 200 mM sodium hydroxide solution. Results demonstrated that sodium sulfite is an effective means either to remove the baseline dip for low concentration analysis under 1 mg/L, or to separate the target sugar from the baseline dip by retention time rearrangement.


Assuntos
Cromatografia por Troca Iônica/métodos , Monossacarídeos/análise , Oxigênio/química , Sulfitos/química , Modelos Lineares , Monossacarídeos/química , Reprodutibilidade dos Testes
19.
Nat Commun ; 10(1): 4065, 2019 09 06.
Artigo em Inglês | MEDLINE | ID: mdl-31492838

RESUMO

Unnatural monosaccharides such as azidosugars that can be metabolically incorporated into cellular glycans are currently used as a major tool for glycan imaging and glycoproteomic profiling. As a common practice to enhance membrane permeability and cellular uptake, the unnatural sugars are per-O-acetylated, which, however, can induce a long-overlooked side reaction, non-enzymatic S-glycosylation. Herein, we develop 1,3-di-esterified N-azidoacetylgalactosamine (GalNAz) as next-generation chemical reporters for metabolic glycan labeling. Both 1,3-di-O-acetylated GalNAz (1,3-Ac2GalNAz) and 1,3-di-O-propionylated GalNAz (1,3-Pr2GalNAz) exhibit high efficiency for labeling protein O-GlcNAcylation with no artificial S-glycosylation. Applying 1,3-Pr2GalNAz in mouse embryonic stem cells (mESCs), we identify ESRRB, a critical transcription factor for pluripotency, as an O-GlcNAcylated protein. We show that ESRRB O-GlcNAcylation is important for mESC self-renewal and pluripotency. Mechanistically, ESRRB is O-GlcNAcylated by O-GlcNAc transferase at serine 25, which stabilizes ESRRB, promotes its transcription activity and facilitates its interactions with two master pluripotency regulators, OCT4 and NANOG.


Assuntos
Acetilglucosamina/metabolismo , Monossacarídeos/metabolismo , Células-Tronco Embrionárias Murinas/metabolismo , Células-Tronco Pluripotentes/metabolismo , Receptores Estrogênicos/metabolismo , Animais , Azidas/química , Azidas/metabolismo , Linhagem Celular Tumoral , Autorrenovação Celular , Células Cultivadas , Glicosilação , Células HeLa , Hexosaminas/metabolismo , Humanos , Células MCF-7 , Camundongos , Monossacarídeos/química , Células-Tronco Embrionárias Murinas/citologia , Células NIH 3T3 , Células-Tronco Pluripotentes/citologia , Processamento de Proteína Pós-Traducional
20.
Chin J Nat Med ; 17(8): 600-607, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31472897

RESUMO

To recognize the potential medicinal value of the Dendrobium sonia, polysaccharide (DSP) was extracted, purified, and investigated for its immunomodulatory activity. In vitro, DSP was shown to enhance the viability (MTT assay) and phagocytosis of macrophages. In cyclophosphamide-induced immunosuppressed mice, DSP increased serum levels of TNF-α, IL-6 and IFN-γ (enzyme-linked immunosorbent assay, ELISA), and ameliorated the imbalance of the community of gut microbiota as detected by 16S ribosomal RNA gene sequencing. These results suggest that DSP might be beneficial for patients under immunosuppressed conditions.


Assuntos
Dendrobium/química , Disbiose/tratamento farmacológico , Microbioma Gastrointestinal/efeitos dos fármacos , Hospedeiro Imunocomprometido , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêutico , Animais , Ciclofosfamida/toxicidade , Citocinas/sangue , Disbiose/induzido quimicamente , Disbiose/imunologia , Fatores Imunológicos/química , Fatores Imunológicos/farmacologia , Fatores Imunológicos/uso terapêutico , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Masculino , Camundongos , Monossacarídeos/química , Fagocitose/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polissacarídeos/química , Células RAW 264.7
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA