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2.
Hum Genet ; 139(2): 257-271, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31942643

RESUMO

Severe asthenozoospermia is a common cause of male infertility. Recent studies have revealed that SPEF2 mutations lead to multiple morphological abnormalities of the sperm flagella (MMAF) without primary ciliary dyskinesia (PCD) symptoms in males, but PCD phenotype was also found in one female individual. Therefore, whether there is a phenotypic continuum ranging from infertile patients with PCD to MMAF patients with no or low noise PCD manifestations remains elusive. Here, we performed whole-exome sequencing in 47 patients with severe asthenozoospermia from 45 unrelated Chinese families. We identified four novel biallelic mutations in SPEF2 (8.9%, 4/45) in six affected individuals (12.8%, 6/47), while no deleterious biallelic variants in SPEF2 were detected in 637 controls, including 219 with oligoasthenospermia, 195 with non-obstructive azoospermia, and 223 fertile controls. Notably, all six patients exhibited PCD-like symptoms, including recurrent airway infections, bronchitis, and rhinosinusitis. Ultrastructural analysis revealed normal 9 + 2 axonemes of respiratory cilia but consistently abnormal 9 + 0 axoneme or disordered accessory structures of sperm flagella, indicating different roles of SPEF2 in sperm flagella and respiratory cilia. Subsequently, a Spef2 knockout mouse model was used to validate the PCD-like phenotype and male infertility, where the subfertility of female Spef2-/- mice was found unexpectedly. Overall, our data bridge the link between MMAF and PCD based on the association of SPEF2 mutations with both infertility and PCD in males and provide basis for further exploring the molecular mechanism of SPEF2 during spermiogenesis and ciliogenesis.


Assuntos
Anormalidades Múltiplas/patologia , Proteínas de Ciclo Celular/genética , Cílios/patologia , Transtornos da Motilidade Ciliar/patologia , Infertilidade Masculina/patologia , Proteínas/fisiologia , Cauda do Espermatozoide/patologia , Anormalidades Múltiplas/genética , Animais , Cílios/genética , Transtornos da Motilidade Ciliar/genética , Feminino , Humanos , Infertilidade Masculina/genética , Masculino , Camundongos , Camundongos Knockout , Fenótipo , Motilidade Espermática , Cauda do Espermatozoide/metabolismo
3.
Ecotoxicol Environ Saf ; 190: 110142, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31911389

RESUMO

Cadmium (Cd) has been reported to induce reproductive toxicity. Recent study indicated that aberrant epigenetic regulation of Multidrug resistance 1b (Mdr1b) causes xenobiotic efflux failure at the blood-testis barrier (BTB). However, whether Mdr1b dysregulation is involved in Cd-mediated dyszoospermia and the underlying mechanism remain unknown. In this study, mice were intragastrically administered 0 or 2.5 mg/kg CdCl2 every other day for 2 months to investigate changes in spermatogenesis and epigenetic regulation of Mdr1b. Mouse Leydig cells TM3 were cultured to detect Mdr1b expression localization. We found that the Cd group revealed BTB disruption concomitant with obvious sperm abnormity and dynamic impairment. Hypermethylation and decreased nuclear factor Ya (Nfya) recruitment to the Mdr1b promoter were correlated with low sperm motility in response to Cd. In conclusion, these findings provide in vivo evidence that epigenetic dysregulation of Mdr1b in the BTB is a potential cause of dyszoospermia upon Cd exposure.


Assuntos
Cádmio/toxicidade , Poluentes Ambientais/toxicidade , Espermatogênese/efeitos dos fármacos , Animais , Barreira Hematotesticular/metabolismo , Fator de Ligação a CCAAT , Núcleo Celular/metabolismo , Resistência a Múltiplos Medicamentos , Epigênese Genética , Infertilidade Masculina , Masculino , Camundongos , Regiões Promotoras Genéticas , Motilidade Espermática , Espermatozoides/metabolismo , Testículo/metabolismo
4.
Life Sci ; 242: 117250, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31899225

RESUMO

BACKGROUND: Endocrine disruptor such as cadmium has been widely reported to cause testicular toxicity, which contributes to recent decline in male fertility worldwide. Glutamine, the most abundant amino acid in the body has been demonstrated to exert protective effects in cellular toxicity. However, its role in testicular toxicity is unknown. The present study is therefore aimed at investigating the effects of glutamine supplementation on cadmium-induced testicular toxicity, and the possible involvement of glucose-6-phosphate dehydrogenase (G6PD) activity. MATERIALS AND METHOD: Male Wistar rats weighing 160-190 g were allotted into 4 groups (n = 5/group): The groups received vehicle (distilled water; p.o.), glutamine (1gkg-1; p.o.), cadmium chloride (5mgkg-1p.o.) and Cadmium chloride plus glutamine respectively, daily for 30 days. Biochemical and histological analyses were performed with appropriate method. RESULTS: Administration of cadmium significantly decreased body weight, sperm count, motility and viability, as well as altered sperm morphology and progressivity. Cadmium also caused atrophy of the seminiferous tubule in addition to disrupted testicular architecture, lumen, Sertoli cells and spermatogonia. Similarly, serum and testicular aspartate transaminase, and malondialdehyde significantly increased, and G6PD, glutathione, nicotinamide adenine dinucleotide phosphate and nitric oxide significantly decreased with corresponding decrease in follicle stimulating hormone, luteinizing hormone and testosterone in cadmium-treated animals compared with control groups. However, supplementation with glutamine attenuated these alterations. CONCLUSION: The present study demonstrates that cadmium induces testicular dysfunction that is attributable to defective G6PD and accompanied by increased lipid peroxidation and impaired NO-dependent endothelial function. Interestingly, glutamine supplementation ameliorates cadmium-induced testicular dysfunction through enhancement of G6PD activity.


Assuntos
Cloreto de Cádmio/toxicidade , Glucosefosfato Desidrogenase/metabolismo , Glutamina/farmacologia , Testículo/efeitos dos fármacos , Animais , Glucosefosfato Desidrogenase/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Espermatozoides/ultraestrutura , Testículo/enzimologia
6.
Toxicol Lett ; 319: 1-10, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31689472

RESUMO

Chlorocholine chloride (CCC), a plant growth retardant, may act as an endocrine disruptor. Our previous study showed that pubertal CCC exposure in rats might decrease testosterone (T) synthesis. This study observed the changes in pubertal development and reproduction of male rats exposed to CCC and its underlying mechanisms. Rats were exposed to CCC (0, 75, 137.5 and 200 mg/kg bw/day) from postnatal day 23 to 60. The results showed that CCC treatment delayed the onset of puberty and reduced the relative organ weight of prostate. Seminiferous tubules with deciduous spermatogenic cells were observed in the 200 mg/kg bw/day group. Sexual behavior was inhibited in the 137.5 and 200 mg/kg bw/day groups. Sperm motility, litter size and normalized anogenital distance (AGD) of male pups were decreased in the 137.5 and 200 mg/kg bw/day groups. Serum kisspeptin level and serum and testicular levels of T were reduced in all CCC treated groups. Crucial hormones in hypothalamic-pituitary-testicular (HPT) axis were reduced subsequently after CCC treatment. Collectively, our results demonstrated that CCC might disturb HPT axis through suppressing the secretion of kisspeptin and subsequently lead to delayed puberty onset and impaired reproductive functions.


Assuntos
Clormequat/toxicidade , Reprodução/efeitos dos fármacos , Maturidade Sexual/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Genitália/anatomia & histologia , Genitália/efeitos dos fármacos , Genitália/crescimento & desenvolvimento , Hormônios Esteroides Gonadais/sangue , Kisspeptinas/metabolismo , Tamanho da Ninhada de Vivíparos/efeitos dos fármacos , Masculino , Tamanho do Órgão/efeitos dos fármacos , Próstata/efeitos dos fármacos , Próstata/crescimento & desenvolvimento , Ratos , Ratos Sprague-Dawley , Túbulos Seminíferos/efeitos dos fármacos , Comportamento Sexual Animal/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Espermatogênese/efeitos dos fármacos , Testosterona/sangue
7.
J Dairy Sci ; 103(1): 545-555, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31668445

RESUMO

The aim of this study was to estimate genetic parameters and genetic trends for male fertility in Norwegian Red bulls. We analyzed data on semen characteristics traits collected at the performance test station of young bulls from 1994 to 2016, in an andrology test used to ensure acceptable semen quality before being selected as an artificial insemination bull. Traits included were volume, concentration, and motility (percentage of moving sperm cells) in fresh samples and after storing for 24 and 48 h, and sperm defects. The data consisted of 14,972 ejaculates from 3,927 young (11-15 mo) Norwegian Red bulls. Genetic parameters were estimated using bivariate linear animal models that included age in months, group-year, and collection-group (main effect of the interaction between ejaculate number and interval between collections) as fixed effects, and test-day and additive genetic and permanent environment effect of the bull as random effects. Considerable genetic coefficients of variation were found for concentration and volume, with lower values for motility. Estimated heritabilities ranged from 0.02 and 0.03 (for sperm defects and motility in fresh samples) to 0.14 (volume and concentration measured on a continuous scale). All estimated genetic correlations were favorable, but the genetic correlations between volume and concentration and volume and sperm defects were not significantly different from zero. The genetic correlations between concentration and motility traits ranged from 0.53 to 0.83, and those between volume and the motility traits were between 0.24 and 0.57. All traits showed a slightly unfavorable genetic trend. Our results indicate that selection of bulls with better sperm quality is possible.


Assuntos
Bovinos/genética , Análise do Sêmen/veterinária , Sêmen , Animais , Inseminação Artificial/veterinária , Masculino , Noruega , Motilidade Espermática , Espermatozoides
8.
J Ethnopharmacol ; 246: 112240, 2020 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-31526861

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: You-Gui-Yin (YGY) is a famous Chinese traditional medicine compound that has been used to treat renal function diseases for more than 300 years. It is recorded in Jing Yue Quanshu, which was written by a famous medical scientist named Jiebing Zhang in the Ming Dynasty. AIM OF THE STUDY: Reproductive dysfunction is one of the most serious complications of chronic kidney disease (CKD). The aim of this study was to observe the effect of You-Gui-Yin (YGY) on reproductive dysfunction of male rats with adenine-induced CKD and to determine if any effects occurred via regulation of the HIF1α-STAT5 pathway. MATERIALS AND METHODS: UPLC-Q-TOF-MS was used to detect the main medicinal components and conduct quality control of YGY. A total of 60 rats were randomly divided into 2 groups: the NC group (10 rats) and the CKD model group (50 rats). The CKD model rats was established by administration of adenine 150 mg kg-1 orally for 14 days. After that, the CKD rats were randomly divided into 5 groups: the CKD group, YGY (10 g kg-1 group, 20 g kg-1 group, 40 g kg-1 group) and the GUI-LU-ER-XIAN-JIAO (GL) 10 g kg-1 group with 10 rats in each group. From the 15th day to the 45th day rats were given 150 mg kg-1 adenine orally every other day to maintain the model (except in the NC group). The YGY groups and the GL group were orally administered the relevant drug once per day for 30 days. The NC group and the CKD group were orally administered an equal volume of normal saline for 30 days. On the 45th day, the rats' sexual behavior index was tested. On the 46th day, the rats were sacrificed. Biochemical indexes, histopathological changes of the kidneys and testes, sperm morphology, sperm abnormality rate, and key proteins in the HIF1α-STAT5 pathway in the kidney and testis were detected. RESULTS: Thirteen components in the YGY extract were identified by UPLC-Q-TOF-MS for quality control of the YGY extract. The results of the biochemical and physiological tests validated the success of inducing CKD accompanied by reproductive dysfunction in rats. YGY significantly retarded the CKD progression and improved the hormone levels of male CKD rats. Sexual behavior tests showed YGY can significantly improve CKD rats' sexual function. In addition, the pathological changes of the kidney and testis, sperm abnormality rate and sperm morphological abnormalities of the CKD rats were reduced by YGY. Furthermore, decreased expression of HIF1α and EPO, and increased expression of p-EPOR (Tyr368), p-JAK2 (Tyr570) and p-STAT5 (Ser725) were observed in the kidney and the testis of the CKD rats. The YGY extract dramatically increased the expression of HIF1α and EPO, and decreased the expression of p-EPOR (Tyr368), p-JAK2 (Tyr570) and p-STAT5 (Ser725) to regulate key proteins in the HIF1α-STAT5 pathway of the kidney and testis. CONCLUSIONS: YGY has obvious reversal effects on the abnormal symptoms of adenine-induced CKD and the abnormal symptoms of rats with hypothyroidism and male reproductive hypotension. Its mechanism is related to its ability to regulate the HIF1α-STAT5 pathway.


Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Insuficiência Renal Crônica/tratamento farmacológico , Fator de Transcrição STAT5/metabolismo , Comportamento Sexual Animal/efeitos dos fármacos , Animais , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Masculino , Medicina Tradicional Chinesa , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Fator de Transcrição STAT5/genética , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
9.
Theriogenology ; 141: 16-25, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31494458

RESUMO

The possible impact of natural heat stress on animal fertility is currently a major concern for breeding companies. Here, we aimed to address this concern by determining the effects of natural heat stress on the fertility of Holstein bulls located in the Netherlands. Semen samples were collected from six bulls at two locations in March 2016 (low temperature-humidity index (THI) group; maximum THI of 51.8 and 55 at their respective locations) or August (high THI group; maximum THI of 77.9 and 80.5 during meiotic and spermiogenic stages of spermatogenesis, 42 to 14 days prior to semen collection). The effect of heat stress on semen quality was assessed by sperm morphology, motility, reactive oxygen species production, lipid peroxidation, viability, and DNA fragmentation. Moreover, we evaluated the development of embryos generated in vitro by low and high THI semen, and determined inner cell mass/trophectoderm ratio, apoptotic cell ratio, and embryonic gene expression in day-8 blastocysts. An increase in cell death (propidium iodide-positive cells; P = 0.039) was observed in the high THI group (31.5%) compared to the low THI group (27.6%). Moreover, a decrease (P < 0.001) was observed in the total blastocyst rates at day 7 post-insemination (15.3 vs 20.9%) and day 8 (23.2 vs 29.6%) in the high THI compared to the low THI group, respectively. There were no differences in the relative abundance of candidate transcripts examined. In conclusion, sperm samples from dairy bulls obtained during a period with higher THI had reduced viability and led to a decrease in blastocyst development and delayed hatching, compared to semen collected during a period with low THI.


Assuntos
Bovinos/fisiologia , Técnicas de Cultura Embrionária/veterinária , Fertilização In Vitro/veterinária , Temperatura Alta , Umidade , Espermatogênese , Animais , Bovinos/embriologia , Sobrevivência Celular , Desenvolvimento Embrionário , Regulação da Expressão Gênica , Masculino , Motilidade Espermática , Espermatozoides
10.
J Pharm Biomed Anal ; 177: 112888, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31563758

RESUMO

Metabolomics measurements of seminal plasma are widely used in diagnosis and finding of molecular mechanisms of male infertility. However, so far the limitation of metabolome coverage of analytical methods hinders comprehensive metabolite biomarker finding. Moreover, the widely used case-control comparison is not enough to unveil the detailed correlations of the metabolic changes with different sperm abnormalities. In this work, we aimed to have comprehensive metabolic profiling of seminal plasma to find the metabolomics difference between healthy controls and infertility case samples with different semen abnormities by liquid chromatography-mass spectrometry (LC-MS) detection with previously established new sample preparation procedure. Among 624 detected metabolite features, 63 potential biomarkers in various metabolite classes were found for infertility in seminal plasma by multivariate analysis. Interestingly, different infertility forms have different potential biomarkers with few in common, and most of potential biomarkers were found in oligo-astheno-teratospermia samples. To further find the association of the metabolomic changes with specific sperm abnormality, sperm parameters including sperm concentration, sperm deformity rate and sperm motility were also collected, and multivariate linear regression was used to find correlations between sperm parameters and potential biomarkers. Finally, levels of 17 metabolites were found to be significantly correlated with sperm parameters. Most of correlations agreed with previously reported mechanisms of infertility, such as correlation of acylcarnitines with sperm concentration and sperm deformity, and correlation of some antioxidants with sperm deformity rate and sperm motility. Some correlations were reported for the first time, such as negative correlations of isopentenyl pyrophosphate, 2-phosphoglyceric acid and γ-glutamyl-Se-methylselenocysteine with sperm deformity rate, and negative correlation of creatine riboside with sperm concentration. All the potential biomarkers were involved in 14 metabolic pathways playing important role in energy production, antioxidation, hormone regulation and sperm membrane. These results proved previously reported molecular mechanism (such as oxidative stress and energy production) and also gave new possible clues to the pathology of male infertility, which will benefit future etiology, diagnosis and treatment of male infertility.


Assuntos
Infertilidade Masculina/diagnóstico , Sêmen/metabolismo , Espermatozoides/patologia , Adulto , Biomarcadores/metabolismo , Estudos de Casos e Controles , Membrana Celular/patologia , Metabolismo Energético , Voluntários Saudáveis , Humanos , Infertilidade Masculina/patologia , Masculino , Metaboloma , Metabolômica/métodos , Estresse Oxidativo , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides/citologia , Espermatozoides/metabolismo , Adulto Jovem
11.
J Ethnopharmacol ; 247: 112269, 2020 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-31610261

RESUMO

ETHNOPHARMACOLOGICAL RELEVANCE: Cisplatin (CP) is the classical chemotherapeutic drug for various cancer, but it also accompanies reproductive toxicity and genotoxicity. Liuwei Dihuang Pill (LW) is the traditional Chinese medicine prescription for treating Kidney-Yin deficiency syndrome, which has been reported to prevent and treat various diseases. However, the protective effect of LW on CP-induced reproductive toxicity and genotoxicity has not been reported. AIM OF THE STUDY: To investigate the potential protective effect and mechanism of LW on CP-induced reproductive toxicity and genotoxicity in male mice. MATERIALS AND METHODS: Mice were given LW (0.4, 1.2 and 3.6 g/kg) or Vitamin C (0.1 g/kg) once daily by oral gavage for thirteen consecutive days. Then, CP (3.00 mg/kg) was given intraperitoneal injection once daily for five consecutive days starting on the ninth day. The protective effects of LW against CP-induced reproductive toxicity and genotoxicity were evaluated by body weight, testis ratio, sperm count, sperm viability, sperm abnormal morphology type, micronuclei test, testicular histopathology, serum malondialdehyde (MDA), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) level. RESULTS: The results demonstrated that LW could significantly increase CP-induced the reduction of sperm count and sperm viability, then decrease abnormal sperm type rate and micronucleus rate. Moreover, LW also could improve testicular abnormal histopathologic morphology induced by CP exposure. Meanwhile, LW decreased serum MDA level and increased T-SOD, GSH-Px and CAT level compared to CP group. CONCLUSION: our findings show that LW has protective effects on CP-induced reproductive toxicity and genotoxicity. LW decreases serum MDA level and increases T-SOD, GSH-Px and CAT level, which indicates that antioxidant activity may be the potential mechanism of LW to resist reproductive toxicity and genotoxicity.


Assuntos
Antioxidantes/farmacologia , Cisplatino/efeitos adversos , Medicamentos de Ervas Chinesas/farmacologia , Substâncias Protetoras/farmacologia , Animais , Dano ao DNA/efeitos dos fármacos , Humanos , Infertilidade/induzido quimicamente , Infertilidade/prevenção & controle , Masculino , Camundongos , Testes para Micronúcleos , Modelos Animais , Estresse Oxidativo/efeitos dos fármacos , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Espermatozoides/patologia , Testículo/efeitos dos fármacos , Testículo/patologia
12.
PLoS Biol ; 17(12): e3000559, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31877125

RESUMO

The global rise in obesity and steady decline in sperm quality are two alarming trends that have emerged during recent decades. In parallel, evidence from model organisms shows that paternal diet can affect offspring metabolic health in a process involving sperm tRNA-derived small RNA (tsRNA). Here, we report that human sperm are acutely sensitive to nutrient flux, both in terms of sperm motility and changes in sperm tsRNA. Over the course of a 2-week diet intervention, in which we first introduced a healthy diet followed by a diet rich in sugar, sperm motility increased and stabilized at high levels. Small RNA-seq on repeatedly sampled sperm from the same individuals revealed that tsRNAs were up-regulated by eating a high-sugar diet for just 1 week. Unsupervised clustering identified two independent pathways for the biogenesis of these tsRNAs: one involving a novel class of fragments with specific cleavage in the T-loop of mature nuclear tRNAs and the other exclusively involving mitochondrial tsRNAs. Mitochondrial involvement was further supported by a similar up-regulation of mitochondrial rRNA-derived small RNA (rsRNA). Notably, the changes in sugar-sensitive tsRNA were positively associated with simultaneous changes in sperm motility and negatively associated with obesity in an independent clinical cohort. This rapid response to a dietary intervention on tsRNA in human sperm is attuned with the paternal intergenerational metabolic responses found in model organisms. More importantly, our findings suggest shared diet-sensitive mechanisms between sperm motility and the biogenesis of tsRNA, which provide novel insights about the interplay between nutrition and male reproductive health.


Assuntos
Dieta/métodos , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Adulto , Humanos , Masculino , Obesidade/metabolismo , RNA/efeitos dos fármacos , RNA/genética , RNA de Transferência/efeitos dos fármacos , RNA de Transferência/genética , Motilidade Espermática/fisiologia , Espermatozoides/metabolismo , Espermatozoides/fisiologia
13.
Clín. investig. ginecol. obstet. (Ed. impr.) ; 46(4): 148-153, oct.-dic. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-184302

RESUMO

Introducción: La solicitud de semen donado ha aumentado en los últimos años, lo que ha llevado a evaluar con mayor rigurosidad las muestras de semen de los donantes. Sin embargo, estas muestras presentan una variabilidad debido a su biología, a la abstinencia sexual, a la actividad de las glándulas accesorias, a los errores analíticos, entre otros. Por lo tanto, el objetivo de este estudio fue evaluar la variación de los parámetros seminales de 15 voluntarios pertenecientes a un programa de donación del Centro de Medicina Reproductiva CONCEVIDAS. Materiales y métodos: Se analizaron los parámetros seminales convencionales de 384 muestras de semen provenientes de 15 donantes. Resultados: Se encontró una mayor variabilidad tanto intra como interindividuo en la concentración total de espermatozoides, en contraste, la viabilidad y la movilidad progresiva fueron los parámetros con menor variación. Conclusión: Este trabajo reafirma la variabilidad biológica del análisis seminal convencional en un grupo de individuos aparentemente sanos


Introduction: The request for donated semen has increased in recent years, which has led to a more rigorous evaluation of donor semen samples. However, the parameters of these samples vary due to their biology, sexual abstinence, the activity of accessory glands, and analytical errors, among others. Therefore, the objective of this study was to evaluate the variation in the seminal parameters of 15 volunteers of a sperm donation program from the reproductive medicine centre CONCEVIDAS. Materials and methods: An analysis was performed on the conventional seminal parameters of 384 semen samples from 15 donors. Results: A wide variation was found in the total sperm concentration. In contrast, viability and sperm progressive motility were the parameters with the least variation. Conclusion: This work confirms the biological variability of conventional seminal analysis in a group of apparently healthy individuals


Assuntos
Humanos , Masculino , Adulto , Sêmen/fisiologia , Análise do Sêmen/estatística & dados numéricos , Espermatozoides/fisiologia , Motilidade Espermática/fisiologia , Técnicas Reprodutivas/estatística & dados numéricos , Sêmen/metabolismo , Fertilização In Vitro
14.
Photochem Photobiol Sci ; 18(12): 2893-2900, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31681924

RESUMO

Photo-modulation with visible light has been used to induce gains in the motility of the sperms of rabbits, boars, buffalo, bulls, fish, and humans. Although different hypotheses have been proposed to explain such an effect, the origin and mechanisms by which visible light affects sperm motility are still far from being completely understood. Several groups have observed changes in the intracellular Ca2+ concentration and significant differences in the production of ROS, which are attributed to specific photosensitizers. Also, it has been reported that blue light induces nitric oxide production in sperm cells, which plays a vital role in acrosome reaction and capacitation leading to an augmentation in the fertilisation probability. In the present work, we study the effects of green light (490-540 nm) on the sperm motility of mice. Firstly, we carried out experiments at 37 °C to confirm what previous researchers have observed before using red and blue light: that the overall sperm motility increases. Secondly, we studied the effects of green light at 10 °C and found that the motility drastically diminishes. In order to understand this opposing outcome, we carried out fluorescence measurements to evaluate reactive oxygen species production induced by green light at both temperatures. Our results suggest that the balance between the use and generation of ROS at 37 °C is favorable to the cells, while at 10 °C it is harmful.


Assuntos
Luz , Motilidade Espermática/efeitos da radiação , Espermatozoides/metabolismo , Animais , Masculino , Camundongos , Espécies Reativas de Oxigênio/metabolismo , Espermatozoides/efeitos da radiação , Temperatura Ambiente
15.
Arch Esp Urol ; 72(9): 939-947, 2019 Nov.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-31697255

RESUMO

During their journey through the female reproductive tract to reach the oocyte in the ampulla of the fallopian tube, spermatozoa interact with substances and microorganisms that affect sperm quality, thus altering their fertilizing capacity. OBJECTIVES: To determine in vitro the effect of Streptococcus agalactiae, Klebsiella pneumoniae and their soluble factors on sperm parameters, and to evaluate the ability of human sperm to interact with and transport these bacteria. METHODS: The effects of S. agalactiae, K. pneumoniae and their soluble factors on the viability, sperm motility and functional sperm parameters were quantified. In addition, motile spermatozoa were incubated with decreasing concentrations of bacteria for one hour, washed and post-infection treatments were performed with trypsin and transport capacity was assessed by quantitative cultures. RESULTS: Incubation of spermatozoa with K. pneumoniae decreased progressive motility. The soluble factors of K. pneumoniae increased the number of necrotic spermatozoa and the soluble factors of S. agalactiae increased lipid peroxidation of the sperm membrane (p<0.05). A strong interaction between sperm and bacteria was observed in the transport assays even in washed trypsin-treated samples. CONCLUSION: Human spermatozoa act as vectors for infections, generating strong interactions with K. pneumoniae and S. agalactiae favoring their diffusion through the female reproductive tract. This interaction affects male fertility by altering progressive motility, increasing the number of necrotic cells and inducing apoptosis.


Assuntos
Espermatozoides , Infecções Estreptocócicas , Streptococcus agalactiae , Feminino , Humanos , Klebsiella pneumoniae/isolamento & purificação , Masculino , Motilidade Espermática , Espermatozoides/microbiologia , Infecções Estreptocócicas/transmissão , Streptococcus agalactiae/isolamento & purificação
16.
Environ Sci Pollut Res Int ; 26(34): 35253-35265, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31701422

RESUMO

Difenoconazole is a fungicide extensively used in agriculture. The aim of this study was to evaluate the effects of difenoconazole fungicide on the sperm quality of rats. Wistar rats were divided into four groups: control and exposed to 5 (D5), 10 (D10), or 50 mg-1 kg bw-1day (D50) of difenoconazole for 30 days, by gavage. Classical sperm parameters and surface-enhanced Raman scattering (SERS) were performed. Progressive motility, acrosomal integrity, and percentage of morphologically normal spermatozoa were reduced in the D10 and D50 groups in comparison with the control group. Sperm viability was reduced only in the D50 group. Sperm number in the testis and caput/corpus epididymis and daily sperm production were reduced in the three exposed groups. SERS measurements showed changes in the spectra of spermatozoa from D50 group, suggesting DNA damage. In addition, machine learning (ML) methods were used to evaluate the performance of three classification algorithms (artificial neural network-ANN, K-nearest neighbors-K-NN, and support vector machine-SVM) in the identification task of the groups exposed to difenoconazole. The results obtained by ML algorithms were very promising with accuracy ≥ 90% and validated the hypothesis of the exposure to difenoconazole reduces sperm quality. In conclusion, exposure of rats to different doses of the fungicide difenoconazole may impair sperm quality, with a recognizable classification pattern of exposure groups.


Assuntos
Dioxolanos/toxicidade , Fungicidas Industriais/toxicidade , Aprendizado de Máquina , Espermatozoides/efeitos dos fármacos , Triazóis/toxicidade , Animais , Dano ao DNA , Epididimo/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar , Análise Espectral Raman , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Máquina de Vetores de Suporte , Testículo/efeitos dos fármacos , Testes de Toxicidade
17.
Environ Sci Pollut Res Int ; 26(33): 34575-34583, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31650475

RESUMO

The aim of the present study was to investigate whether curcumin (CUR) can ameliorate cadmium-induced reproductive toxicity and its mechanism. A total of 48 male mice were equally divided into 4 groups: control, CdCl2 (2 mg/kg, intraperitoneally inject) curcumin (50 mg/kg, intraperitoneally inject), co-treatment with curcumin (50 mg/kg), and CdCl2 (2 mg/kg) for 10 days. The results demonstrated that CdCl2 reduces sperm motility, decreases the sperm density and serum testosterone content, and significantly improves the rate of sperm deformity. CdCl2 increased the level of testicular total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activity, and glutathione (GSH), and CdCl2 declined the level of malondialdehyde (MDA). However, the semen quality of the mice in the curcumin intervention group was improved. Moreover, the testosterone content and antioxidant capacity were increased. In the Cd group mice, the expression of testicular Nrf2, as well as the mRNA and protein expressions of the downstream target molecules, glutathione peroxidase (GSH-Px), and γ-glutamylcysteine synthetase (γ-GCS) of Nrf2 declined, while the above genetic expressions elevated significantly in the curcumin intervention group. Our results suggested that curcumin could protect against Cd-induced testicular injury via activating the Nrf2/ARE signaling pathway.


Assuntos
Antioxidantes/farmacologia , Cádmio/toxicidade , Curcumina/farmacologia , Testículo/fisiologia , Animais , Antioxidantes/metabolismo , Fator de Transcrição de Proteínas de Ligação GA/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Análise do Sêmen , Transdução de Sinais/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Testículo/efeitos dos fármacos , Testículo/metabolismo , Testosterona/metabolismo
18.
Int Braz J Urol ; 45(5): 1043-1054, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31626524

RESUMO

OBJECTIVE: Anacyclus Pyrethrum (AP) and Tribulus Terrestris (TT) have been reported as male infertility treatment in several studies; however, in Iranian traditional medicine these two plants are prescribed simultaneously. In this study, we aimed to determine the effects of AP and TT extracts both separately and simultaneously on the male Wistar rat fertility parameters. MATERIALS AND METHODS: 32 male Wistar rats were divided into 4 groups: Control, TT, AP, and AT treated groups. Treatment continued for 25 days and rats were weighed daily. Their testes were dissected for histological studies. Sperm analysis including sperm count, viability and motility were performed. Serum was obtained to evaluate testosterone, LH and FSH levels. Histological studies were conducted to study Leydig, and Sertoli cells, spermatogonia and spermatid cell numbers, and to measure seminiferous diameter and epithelium thickness. RESULTS: Sperm count increased in all the treatment groups. Sperm viability and motility in AT and AP groups were elevated. TT and AT groups showed signifi cantly increased testosterone level compared to control group (P=004, P=0.000, respectively) and TT, AP and AT treatment groups showed increased LH level (P=0.002, P=0.03 and P=0.000, respectively) compared to control, while only AT group showed increased FSH (p=0.006) compared to control. Histological studies showed signifi cant increase of spermatogonia, Leydig and Sertoli cell numbers and epithelial thickness in AT group compared to other groups. All the treatment groups had higher number of Leydig, spermatogonia and spermatid cells. CONCLUSION: TT and AP improved sexual parameters; however, their simultaneous administration had higher improving effects on studied parameters.


Assuntos
Chrysanthemum cinerariifolium/química , Infertilidade Masculina/tratamento farmacológico , Extratos Vegetais/uso terapêutico , Tribulus/química , Animais , Peso Corporal , Fertilidade/efeitos dos fármacos , Hormônio Foliculoestimulante/sangue , Humanos , Hormônio Luteinizante/sangue , Masculino , Tamanho do Órgão , Extratos Vegetais/farmacologia , Ratos Wistar , Valores de Referência , Reprodutibilidade dos Testes , Contagem de Espermatozoides , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Testículo/efeitos dos fármacos , Testosterona/sangue , Resultado do Tratamento
19.
Biomed Res Int ; 2019: 5397804, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31531356

RESUMO

For human infertility both male and female factors may be equally important. Searching for molecular biomarkers of male infertility, neglected for decades, and the attempts to explain regulatory mechanisms of fertilization become thus extremely important. Apart from examination of the structure and function of male gametes, also the possible importance of seminal plasma components should be considered. In this article we discuss data that indicate for the substantial significance of active seminal plasma components for conception and achievement of healthy pregnancy. Seminal plasma impact on the storage and cryopreservation of human and animal sperm and regulatory role of glycodelin on human sperm capacitation as well as hypothesized course of female immune response to allogenic sperm and conceptus has been discussed. The possible involvement of carbohydrates in molecular mechanism of fetoembryonic defense has been also mentioned.


Assuntos
Fertilização/fisiologia , Sêmen/fisiologia , Espermatozoides/fisiologia , Animais , Criopreservação/métodos , Humanos , Infertilidade Masculina/fisiopatologia , Masculino , Preservação do Sêmen/métodos , Capacitação Espermática/fisiologia , Motilidade Espermática/fisiologia
20.
Pak J Pharm Sci ; 32(3 Special): 1419-1422, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31551224

RESUMO

To observe and investigate the in vitro effect of ulipristal acetate (UPA) on human sperm parameters and function. The 20 patients with normal semen parameters and average age of (32.5±8.5) years old, who were treated in our hospital from January 2018 to August 2018, were selected as research objects. They were subjected to density gradient centrifugation, and then four groups were incubated for about an hour in a culture medium containing different concentrates of ulipristal acetate and the other two groups were set as blank control group and dimethylsulphoxide (DMSO) control group. Indicators including sperm motility, sperm hyperactivation and sperm concentration of free calcium ions of each group were tested. Under the ulipristal acetate concentration of 0.0.4 mol/L, the proportion of sperm damage was increased, the length of tail was increased, and the proportion of sperm hyperactivation was decreased, p<0.05. In addition, the acrosome reaction was inhibited, which significantly reduced the calcium concentration in the sperm, p<0.05. Ulipristal acetate can significantly inhibit acrosome reaction and hyperactivation of sperm in vitro, and can reduce the concentration of calcium ions in sperm, thus causing sperm damage.


Assuntos
Norpregnadienos/farmacologia , Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologia , Reação Acrossômica/efeitos dos fármacos , Adulto , Cálcio/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Humanos , Masculino , Progesterona/farmacologia , Motilidade Espermática/efeitos dos fármacos
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