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1.
Clín. investig. ginecol. obstet. (Ed. impr.) ; 46(4): 148-153, oct.-dic. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-184302

RESUMO

Introducción: La solicitud de semen donado ha aumentado en los últimos años, lo que ha llevado a evaluar con mayor rigurosidad las muestras de semen de los donantes. Sin embargo, estas muestras presentan una variabilidad debido a su biología, a la abstinencia sexual, a la actividad de las glándulas accesorias, a los errores analíticos, entre otros. Por lo tanto, el objetivo de este estudio fue evaluar la variación de los parámetros seminales de 15 voluntarios pertenecientes a un programa de donación del Centro de Medicina Reproductiva CONCEVIDAS. Materiales y métodos: Se analizaron los parámetros seminales convencionales de 384 muestras de semen provenientes de 15 donantes. Resultados: Se encontró una mayor variabilidad tanto intra como interindividuo en la concentración total de espermatozoides, en contraste, la viabilidad y la movilidad progresiva fueron los parámetros con menor variación. Conclusión: Este trabajo reafirma la variabilidad biológica del análisis seminal convencional en un grupo de individuos aparentemente sanos


Introduction: The request for donated semen has increased in recent years, which has led to a more rigorous evaluation of donor semen samples. However, the parameters of these samples vary due to their biology, sexual abstinence, the activity of accessory glands, and analytical errors, among others. Therefore, the objective of this study was to evaluate the variation in the seminal parameters of 15 volunteers of a sperm donation program from the reproductive medicine centre CONCEVIDAS. Materials and methods: An analysis was performed on the conventional seminal parameters of 384 semen samples from 15 donors. Results: A wide variation was found in the total sperm concentration. In contrast, viability and sperm progressive motility were the parameters with the least variation. Conclusion: This work confirms the biological variability of conventional seminal analysis in a group of apparently healthy individuals


Assuntos
Humanos , Masculino , Adulto , Sêmen/fisiologia , Análise do Sêmen/estatística & dados numéricos , Espermatozoides/fisiologia , Motilidade Espermática/fisiologia , Técnicas Reprodutivas/estatística & dados numéricos , Sêmen/metabolismo , Fertilização In Vitro
2.
Reprod Domest Anim ; 54 Suppl 3: 22-28, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31512320

RESUMO

The biological nature of age-related declines in fertility in males of any species, including stallions, has been elusive. In horses, the economic costs to the breeding industry are frequently extensive. Mitochondrial function in ejaculated sperm, which is essential for sperm motility, is reflected by adenosine triphosphate production, mitochondrial oxidative efficiency and production of reactive oxygen species, and that this balance may become compromised in ageing stallions and during the process of cryopreservation. This presentation will focus on mitochondrial integrity and function as an avenue for understanding the pathophysiology of sperm when undergoing cryopreservation and male ageing. We discuss the importance of understanding the differences and similarities of sperm mitochondria to that of somatic cells regarding structure and mitochondrial biochemistry relating to sperm function. The roles of oxidative phosphorylation and glycolysis in sperm mitochondria are outlined as is the method of determining oxygen consumption and calcium homoeostasis in sperm mitochondria. Further, we outline the role of oxidative stress and reactive oxygen species.


Assuntos
Cavalos/fisiologia , Mitocôndrias/fisiologia , Espermatozoides/fisiologia , Envelhecimento/fisiologia , Animais , Criopreservação/veterinária , Fertilidade/fisiologia , Glicólise , Masculino , Mitocôndrias/metabolismo , Fosforilação Oxidativa , Técnicas de Reprodução Assistida/veterinária , Motilidade Espermática/fisiologia , Espermatozoides/citologia , Espermatozoides/metabolismo
3.
Medicine (Baltimore) ; 98(32): e16677, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31393367

RESUMO

BACKGROUND: Male infertility has become a worldwide public health problem. However, the effect of low body mass index (BMI) is still controversial. METHODS: Relevant articles in Pubmed, Embase, Web of science, and Wanfang database published until September 2017 were searched without language restriction. We performed a meta-analysis about low BMI and semen parameters containing total sperm count, concentration, semen volume, and sperm motility (overall and progressive), including 709 men with low BMI and 14,622 men with normal BMI. RESULTS: Thirteen studies were included in this meta-analysis and a total of 15,331 individuals were accumulated. We pooled data from these articles and found standardized weighted mean differences in semen parameters (total sperm count and semen volume) showed significant difference between low BMI and normal BMI. CONCLUSIONS: This systematic review with meta-analysis has confirmed that there was a relationship between low BMI and semen quality, which suggesting low BMI may be a harmful factor of male infertility. Yet lacking of the raw data may influence the accuracy of the results. Further researches are needed to identify the role of underweight in male sterility.


Assuntos
Contagem de Espermatozoides/estatística & dados numéricos , Motilidade Espermática/fisiologia , Magreza/fisiopatologia , Índice de Massa Corporal , Estudos de Casos e Controles , Humanos , Infertilidade Masculina/etiologia , Masculino , Fatores de Risco , Magreza/complicações
4.
Anim Reprod Sci ; 208: 106126, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405480

RESUMO

The aim of this study was to compare the effect of three sugars and Equex paste in a freezing extender for donkey sperm cryopreservation. Ejaculates (n = 18) were collected from six Andalusian donkeys of proven fertility were pooled (two ejaculates per pool) and cryopreserved using a freezing extender containing three different sugars (glucose, fructose and sorbitol), with or without the addition of Equex paste. Sperm quality was assessed before and after freezing-thawing for motility, morphology, plasma membrane integrity, acrosome integrity and DNA integrity. The use of sorbitol in the freezing extender improved total and progressive sperm motility (P < 0.05) and amplitude of lateral head displacement (P < 0.01), but it reduced the values for other sperm motility variables compared with glucose (P < 0.001). The use of fructose resulted in a reduction in values for most CASA variables (P < 0.05), whereas addition of Equex paste did not have any beneficial effect on values for these variables (P > 0.05). Glucose was more effective in maintaining sperm morphology (P < 0.05), while there was no beneficial effect with the addition of Equex paste (P > 0.05). Supplementation of fructose and Equex paste in the freezing extender decreased plasma membrane integrity (P < 0.05) as compared with glucose, but there were no differences between treatments for acrosome and DNA integrity (P > 0.05), even after 24 h of incubation. The use of different sugar sources in the extender could affect the in vitro post-thaw quality of cryopreserved donkey spermatozoa, with sorbitol being an interesting alternative for improving the sperm quality. Results of the present study indicate the use of Equex paste could negatively affect post-thaw outcomes for sperm viability in this species.


Assuntos
Criopreservação/veterinária , Crioprotetores/farmacologia , Equidae/fisiologia , Preservação do Sêmen/veterinária , Espermatozoides/fisiologia , Animais , DNA/química , Congelamento , Masculino , Análise do Sêmen , Preservação do Sêmen/métodos , Motilidade Espermática/fisiologia
5.
Soft Matter ; 15(31): 6269-6277, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31322640

RESUMO

The trajectory of sperm in the presence of background flow is of utmost importance for the success of fertilization, as sperm encounter background flow of different magnitude and direction on their way to the egg. Here, we have studied the effect of an unbounded simple shear flow as well as a Poiseuille flow on the sperm trajectory. In the presence of a simple shear flow, the sperm moves on an elliptical trajectory in the reference frame advecting with the local background flow. The length of the major-axis of this elliptical trajectory decreases with the shear rate. The flexibility of the flagellum and consequently the length of the major axis of the elliptical trajectories increases with the sperm number. The sperm number is a dimensionless number representing the ratio of viscous force to elastic force. The sperm moves downstream or upstream depending on the strength of background Poiseuille flow. In contrast to the simple shear flow, the sperm also moves toward the centerline in a Poiseuille flow. Far away from the centerline, the cross-stream migration velocity of the sperm increases as the transverse distance of the sperm from the centerline decreases. Close to the centerline, on the other hand, the cross-stream migration velocity decreases as the sperm further approaches the center. The cross-stream migration velocity of the sperm also increases with the sperm number.


Assuntos
Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Flagelos/fisiologia , Humanos , Masculino , Modelos Biológicos , Resistência ao Cisalhamento , Estresse Mecânico
6.
Pol J Vet Sci ; 22(2): 227-235, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31269332

RESUMO

The aim of this study was to identify the proteoforms of albumin and kallikrein in stallion seminal plasma (SP), and to determine their correlations with sperm motility parameters. The experimental material consisted of ejaculates from 8 stallions, which were collected during the breeding and non-breeding seasons (BS and NBS, respectively). SP proteins were identified by 2-D PAGE and mass spectrometry (MALDI TOT/TOF MS). Sperm motility parameters were analyzed using the CASA system. Protein expression (integrated optical density-IOD) of albumin proteoforms 1 (ALB 1) and 2 (ALB 2) and kallikrein proteoforms 1 (KAL 1) and 2 (KAL 2) was correlated (p⟨0.05) with sperm motility parameters (total motility and progressive motility) during the BS. No significant correlations were found between the expression of albumin or kallikrein and sperm motility parameters during the NBS. The presence of correlations between the expression of ALB 1, ALB 2, KAL 1, KAL 2 and selected sperm motility parameters could suggest that the analyzed components of the SP belong to the group of fertility-associated proteins (FAPs).


Assuntos
Albuminas/química , Cavalos , Calicreínas/química , Sêmen/química , Motilidade Espermática/fisiologia , Albuminas/genética , Albuminas/metabolismo , Animais , Calicreínas/genética , Calicreínas/metabolismo , Masculino , Isoformas de Proteínas
7.
Theriogenology ; 136: 143-165, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31265944

RESUMO

Fish spermatozoa acquire potential for motility in the sperm duct where they are immotile. Osmolality of the seminal plasma is a key factor to maintain spermatozoa in the quiescent state in either freshwater or marine fishes. However, potassium (K+) ions prevent spermatozoa motility in salmonid and sturgeon fishes, while CO2 inhibits spermatozoa motility in flatfishes. Once, spermatozoa are released at spawning, their motility is initiated in hypo-osmotic and hyper-osmotic environments in freshwater and marine fishes, respectively. Some substances produced by the testes (a progestin), or released from oocytes (peptides) induce spermatozoa hypermotility in some marine fishes including the Atlantic croaker and Pacific herrings, respectively. Duration of spermatozoa motility is short, lasting for a few seconds to few minutes in most fishes due to rapid depletion of energy required for the beating of the motility apparatus called axoneme. In the osmotic-activated spermatozoa, K+ and water effluxes occur in freshwater and marine fishes, respectively, which trigger spermatozoa motility signaling. In general, initiation of axonemal beating is associated with an increase in intracellular calcium (Ca2+) ions in spermatozoa of both freshwater and marine fishes and a post- or pre-increase in intracellular pH, while cyclic adenosine monophosphate (cAMP) remains unchanged. However, axonemal beating is cAMP-dependent in demembranated spermatozoa of salmonid and sturgeon fishes. Calcium from extracellular environment or intracellular stores supply required Ca2+ concentration for axonemal beating. Several axonemal proteins have been so far identified in fishes that are activated by Ca2+ and cAMP, directly or mediated by protein kinase C and protein kinase A, respectively. The present study reviews differences and similarities in complex regulatory signals controlling spermatozoa motility initiation in fishes, and notes physiological mechanisms that await elucidation.


Assuntos
Peixes/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Animais , Masculino , Transdução de Sinais/fisiologia , Especificidade da Espécie
8.
J Fish Biol ; 95(4): 1137-1144, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31355446

RESUMO

Several factors regulating activation of spermatozoon motility in Eurasian burbot, Lota lota, including osmolality, calcium (Ca2+ ) ions, and temperature were investigated. Spermatozoon motility in Eurasian burbot, Lota lota was assessed at 4 and 30°C in seminal fluid, isotonic media (with and without Ca2+ ) and hypotonic media (with and without Ca2+ ). Spermatozoa were spontaneously activated in seminal fluid at 20°C and the maximum motility was recorded at 30°C, which is out of the spawning temperature range, indicating that no risk of activation occurs during routine semen handling in artificial insemination. Initiation of spermatozoon motility in L. lota is mediated by Ca2+ and sensitivity to Ca2+ is dependent on temperature.


Assuntos
Cálcio/farmacologia , Gadiformes/fisiologia , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos , Animais , Cálcio/metabolismo , Masculino , Concentração Osmolar , Sêmen , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Temperatura Ambiente
9.
Cells ; 8(7)2019 06 27.
Artigo em Inglês | MEDLINE | ID: mdl-31252584

RESUMO

Inside the female genital tract, mammalian sperm undergo a maturation process called capacitation, which primes the sperm to navigate across the oviduct and fertilize the egg. Sperm capacitation and motility are controlled by 3',5'-cyclic adenosine monophosphate (cAMP). Here, we show that optogenetics, the control of cellular signaling by genetically encoded light-activated proteins, allows to manipulate cAMP dynamics in sperm flagella and, thereby, sperm capacitation and motility by light. To this end, we used sperm that express the light-activated phosphodiesterase LAPD or the photo-activated adenylate cyclase bPAC. The control of cAMP by LAPD or bPAC combined with pharmacological interventions provides spatiotemporal precision and allows to probe the physiological function of cAMP compartmentalization in mammalian sperm.


Assuntos
AMP Cíclico/metabolismo , Optogenética/métodos , Capacitação Espermática/fisiologia , Motilidade Espermática/fisiologia , Cauda do Espermatozoide/metabolismo , Animais , Ensaios Enzimáticos , Luz , Masculino , Camundongos , Camundongos Transgênicos , Diester Fosfórico Hidrolases/genética , Diester Fosfórico Hidrolases/metabolismo , Diester Fosfórico Hidrolases/efeitos da radiação , Análise Espaço-Temporal
10.
Theriogenology ; 136: 15-27, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31234053

RESUMO

Though bivalve mollusks are keystone species and major species groups in aquaculture production worldwide, gamete biology is still largely unknown. This review aims to provide a synthesis of current knowledge in the field of sperm biology, including spermatozoa motility, flagellar beating, and energy metabolism; and to illustrate cellular signaling controlling spermatozoa motility initiation in bivalves. Serotonin (5-HT) induces hyper-motility in spermatozoa via a 5-HT receptor, suggesting a serotoninergic system in the male reproductive tract that might regulate sperm physiology. Acidic pH and high concentration of K+ are inhibitory factors of spermatozoa motility in the testis. Motility is initiated at spawning by a Na+-dependent alkalization of intracellular pH mediated by a Na+/H+ exchanger. Increase of 5-HT in the testis and decrease of extracellular K+ when sperm is released in seawater induce hyperpolarization of spermatozoa membrane potential mediated by K+ efflux and associated with an increase in intracellular Ca2+ via opening of voltage-dependent Ca2+ channels under alkaline conditions. These events activate dynein ATPases and Ca2+/calmodulin-dependent proteins resulting in flagellar beating. It may be possible that 5-HT is also involved in intracellular cAMP rise controlling cAMP-dependent protein kinase phosphorylation in the flagellum. Once motility is triggered, flagellum beats in asymmetric wave pattern leading to circular trajectories of spermatozoa. Three different flagellar wave characteristics are reported, including "full", "twitching", and "declining" propagation of wave, which are described and illustrated in the present review. Mitochondrial respiration, ATP content, and metabolic pathways producing ATP in bivalve spermatozoa are discussed. Energy metabolism of Pacific oyster spermatozoa differs from previously studied marine species since oxidative phosphorylation synthetizes a stable level of ATP throughout 24-h motility period and the end of movement is not explained by a low intracellular ATP content, revealing different strategy to improve oocyte fertilization success. Finally, our review highlights physiological mechanisms that require further researches and points out some advantages of bivalve spermatozoa to extend knowledge on mechanisms of motility.


Assuntos
Bivalves/fisiologia , Flagelos/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/citologia , Espermatozoides/fisiologia , Animais , Metabolismo Energético , Masculino , Especificidade da Espécie
11.
Reprod Domest Anim ; 54(8): 1113-1120, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31177582

RESUMO

The objective of this study was to evaluate the accuracy of a novel, portable device (iSperm® Equine for assessing concentration and motility of stallion semen). In the first experiment, semen concentration was determined by the iSperm® Equine (Aidmics Biotechnology), Androvision® (Minitube) and NucleoCounter® SP-100™ (ChemoMetec). The total motility and progressive motility were determined by the iSperm® Equine and the Androvision® using the manufacturer's guidelines. Frozen/thawed semen samples (n = 33) at various dilutions were analysed for concentration and motility with the above-mentioned devices. There was a significant correlation between the concentrations measured with iSperm® and NucleoCounter® at all the measured dilutions. Moreover, <10% difference in concentrations was observed between the iSperm® and NucleoCounter® using the Bland-Altman test. There was also a significant correlation between iSperm® and Androvision® for total and progressive motility. In the second experiment, the parameters used in the Androvision® were modified to match those of the iSperm® . Total motility and progressive motility of frozen/thawed semen samples (n = 10) were determined, and the similarity between the Androvision® and iSperm® was confirmed by correlation studies and Bland-Altman test. The results of these experiments demonstrate that the iSperm® offers a reliable and practical alternative for the semi-automated measurement of concentration and motility of stallion semen in the field. The iSperm® enables the practitioner to obtain objective and repeatable measurements on a variety of semen types (fresh, cooled and frozen) in the field at the time of insemination and thus acquire more insight into the quantity and quality of the provided insemination doses. This mare-side diagnostic tool may help practitioners in identifying presumed subfertility problems more rapidly and act accordingly.


Assuntos
Cavalos , Análise do Sêmen/veterinária , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Animais , Criopreservação/veterinária , Masculino , Análise do Sêmen/instrumentação , Preservação do Sêmen/veterinária
12.
Theriogenology ; 135: 152-163, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31216506

RESUMO

Motility is a characteristic function of the male gamete, which allows spermatozoa to actively reach and penetrate the female gamete in organisms with internal and external fertilization. Sperm motility is acquired under the control of many extrinsic and intrinsic factors and is based on a specialized structure of the sperm flagellum called "axoneme". An overview of how the sperm flagellum is organized, and it operates to support cell motility is presented, with special focus on the molecular mechanisms and factors involved in the development, maintenance and control of motility. Data obtained in aquatic organisms with external fertilization, such as sea urchins, ascidians or fishes are critically analyzed because they constitute model species on which most of the present day understanding of sperm motility function is based. In most animal species, sperm motility is dependent on a long appendage called flagellum. Flagella are essential organelles found in most eukaryotic cells; their basic structure is the axoneme, which consists of a scaffold of microtubules and is responsible for movement in an autonomous manner if ATP-energy is present. Flagellar beat propels the cell through the medium which surrounds sperm cells and is responsible of the translational drive of spermatozoa. The present paper includes: (1) an introduction to typical sperm morphology and ultrastructure in most aquatic species, (2) the motility apparatus or axoneme of the spermatozoa: the axoneme, (3) the structural and biochemical composition of the axoneme, (4) the axonemal motor or dynein, and its operation, (5) the regulation of motility at axoneme and cell membrane levels, including several effectors such as Ca2+ ions, (6) biophysical features of the wave propagation mechanism in motile spermatozoa, (7) the energy production and consumption, and (8) the building of a flagellum. Flagellar beating in aquatic animals is illustrated using several examples in figures and video-clips. These types of data are also used for computer simulation of various aspects of the modulation of sperm motility of marine animals.


Assuntos
Organismos Aquáticos , Flagelos/fisiologia , Invertebrados/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Vertebrados/fisiologia , Animais , Masculino , Especificidade da Espécie
13.
Theriogenology ; 137: 104-112, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31230704

RESUMO

The journey that spermatozoa take following deposition in the female tract is a long and perilous one. The barriers they face within the female tract differ depending on whether they are deposited in the vagina or uterus, like spermatozoa of the ram or boar respectively. Comparative studies on the transit of spermatozoa through the ewe and sow tracts serves to highlight similarities, or differences, in the way their sperm-surface properties enable them to overcome these barriers, progress through the tract and fertilise the oocyte. The female environment contributes towards this successful transit by providing a vehicle for sperm transport, aiding the removal of dead spermatozoa and other pathogens and applying strict selection pressures to ensure only those cells with the highest quality reach the site of fertilisation. Understanding the criteria behind these natural barriers helps an understanding of the limitations to fertility associated with preserved spermatozoa, and how in vitro manipulation can alter this complex interaction between spermatozoa and the female environment. Similar mechanisms or surface coat interactions exist in both species, but each has evolved to be used for physiologically disparate functions. Here we briefly describe the sperm surface characteristics of both fresh and frozen-thawed boar and ram spermatozoa and compare how these properties equip them to survive the physical, biochemical and immune interactions within the female reproductive tract.


Assuntos
Genitália Feminina/fisiologia , Espermatozoides/fisiologia , Animais , Feminino , Masculino , Sêmen/fisiologia , Especificidade da Espécie , Motilidade Espermática/fisiologia
14.
Theriogenology ; 136: 47-59, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31247386

RESUMO

Mithun (Bos frontalis) is a unique domestic free range bovine species of North Eastern Hilly (NEH) regions of India. Effect of feed supplementation of Flaxseed oil (FSO) on semen production and its quality profiles, freezability, oxidative stress, apoptotic sperm percentage and subsequently on endocrinological profiles & scrotal and testicular biometrics in different seasons was studied in mithun. The experimental animals were divided into two groups, Gr I: Control (n = 3) and Gr II: Treatment (n = 3; Flaxseed oil @ 150 mL/day). FSO was supplemented through oral drench in the morning hours just before concentrate feeding. A total of 80 semen samples (n = 80; 20 semen samples from each season; each 10 semen samples from control and treatment groups per season) were collected, not more than twice per week in winter, spring, autumn and summer seasons. Semen quality profiles (SQPs) such as volume, sperm concentration, motility (forward progressive and total), motility & velocity profiles by computer assisted sperm analyser (CASA), viability, total sperm abnormality, acrosome integrity, plasma membrane & nuclear abnormality and apoptotic sperm percentage were estimated in fresh semen. Along with SQPs measured in fresh semen, motility in estrus bovine cervical mucus (bovine cervical mucus penetration test; BCMPT) and mitochondrial membrane potential (MMP) by JC-1 stain were determined in the post-thawed semen samples. Biochemical profiles (aspartate aminotransferase; AST, alanine aminotransferase; ALT, total cholesterol; CHO), antioxidant profiles (superoxide dismutase; SOD, catalase; CAT, glutathione; GSH, total antioxidant capacity; TAC) and oxidative stress profile (malondialdehyde; MDA) were estimated in fresh semen whereas AST, ALT, lactate dehydrogenase (LDH), TAC and MDA were estimated in the frozen thawed semen samples. Endocrinological profiles such as follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, cortisol and thyroxin and scrotal circumference (SC) & testicular biometrics were measured in both groups in different seasons. Result revealed a significant (p < 0.05) improvement in motility (total & forward progressive, motility & velocity by CASA and vanguard distance in cervical mucus), viability, intactness of acrosome & plasma membrane, MMP, antioxidant profiles and reduction in total sperm and nuclear abnormalities, reduction in leakage of intracellular enzymes and reduction in oxidative stress profile and reduction of apoptotic sperm percentage were observed in FSO supplemented than in un-supplemented control group accordingly in fresh and post thawed semen samples. Blood FSH, LH, testosterone and thyroxin concentration were significantly (p < 0.05) increased and cortisol concentration was significantly (p < 0.05) decreased in FSO supplemented group than in unsupplemented control group. Similarly, SC and testicular biometrics were increased significantly (p < 0.05) in supplemented than unsupplemented group for different seasons and significantly (p < 0.05) higher in winter and spring than in summer season in the experimental groups. It can be concluded from the study that supplementation of FSO can effectively be utilized to improve the antioxidant profiles, reduction of oxidative stress with cascading beneficial effects on SQPs and fertility status of the mithun bull.


Assuntos
Óleo de Semente do Linho/farmacologia , Análise do Sêmen/veterinária , Sêmen/efeitos dos fármacos , Administração Oral , Animais , Bovinos , Sobrevivência Celular , Criopreservação/veterinária , Processamento de Imagem Assistida por Computador , Óleo de Semente do Linho/administração & dosagem , Masculino , Motilidade Espermática/efeitos dos fármacos , Motilidade Espermática/fisiologia , Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologia
15.
Eur J Obstet Gynecol Reprod Biol ; 237: 170-174, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31063967

RESUMO

OBJECTIVE: To determine the implication of general physical activity and some specific sports in semen quality in men from infertile couples. STUDY DESIGN: This is an observational study performed in men from infertile couples (n = 454). The interventions performed involved analyzing semen quality parameters according to 2010 WHO criteria and assessing physical activity by means of an International Physical Activity Questionnaire. RESULT(S): There was no association between different levels of general physical activity and semen parameters. We neither found association with running, cycling and racquet sports. Interestingly, people who practice weightlifting more than two hours per week presented significantly lower sperm concentration (linear coefficient = -24.80) and lower total sperm count (linear coefficient = -70.87) in comparison with participants that did not practice regular exercise. CONCLUSION(S): From a reproductive point of view, there does not seem to be any reason to recommend the increase or the decrease in general physical activity in males from infertile couples. However, additional studies are needed to investigate the relationship between weightlifting and sperm quality.


Assuntos
Exercício/fisiologia , Infertilidade/fisiopatologia , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia , Adulto , Humanos , Masculino , Análise do Sêmen , Contagem de Espermatozoides
16.
J Assist Reprod Genet ; 36(7): 1401-1412, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31079268

RESUMO

PURPOSE: Increasing intracellular energy storage by chemically activating adenosine monophosphate-activated protein kinase (AMPKα) prior to sperm cryopreservation may improve post-thawed sperm function. Using the domestic cat as a biomedical model, the objectives were to (1) confirm the expression of AMPKα and its regulatory kinases in epididymal spermatozoa and (2) assess the influence of AMPK activator, 5'-aminoimidasole-4-carboxamide-1-ß-d-ribofuranoside (AICAR) on epididymal sperm function before and after cryopreservation. METHODS: In study I, sperm samples of different qualities were obtained from cauda epididymides of domestic cats and evaluated for AMPKα expression. In study II, epididymal spermatozoa were equilibrated for either 30 or 60 min in the presence of 0 (control), 0.5, 2.0, and 5.0 mM AICAR and sperm functions were assessed before and after cryopreservation. In study III, epididymal spermatozoa were treated as in study II and evaluated for AMPKα signaling protein expressions (phospho-AMPKα Thr172 and GLUT1) as well as ATP levels. RESULTS: AMPKα protein expression was higher in high-motility vs poor-motility samples. Thirty-minute equilibration with 0.5 mM AICAR improved motion characteristics and fertilizing ability of cryopreserved sperm to the control. Increased expressions of phospho-AMPKα Thr172 and GLUT1 as well as intracellular ATP level were confirmed in sperm samples equilibrated with 0.5 or 2.0 mM AICAR for 30 min. CONCLUSIONS: Presence and role of AMPKα protein in cat regulating sperm function were demonstrated before and after cryopreservation. Findings could be used to potentially enhance cryopreserved sperm function in sub-fertile men.


Assuntos
Criopreservação , Metabolismo Energético/genética , Proteínas Quinases/genética , Espermatozoides/crescimento & desenvolvimento , Animais , Gatos , Feminino , Fertilização/genética , Fertilização/fisiologia , Humanos , Masculino , Preservação do Sêmen/métodos , Motilidade Espermática/genética , Motilidade Espermática/fisiologia , Espermatozoides/metabolismo
17.
PLoS One ; 14(5): e0209950, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31086364

RESUMO

Vaginal lubricants are commonly used by couples trying-to-conceive. However, most vaginal lubricants are sperm toxic and therefore should not be used by couples trying-to-conceive. Despite this, lubricant sperm toxicity is insufficiently reported and guidance for healthcare professionals (HCPs) is absent. In this study, lubricant-related practices of fertility-based HCPs in Scotland were sampled via an online survey. Lubricants identified as being utilised in the fertility setting were subsequently incubated with prepared sperm samples to establish effects on sperm motility. HCP recommendations (n = 32) on lubricant use were varied although knowledge related to sperm toxicity was generally poor. HCPs infrequently asked about lubricant use and were unaware of guidance in this area. Aquagel, the only prescribed lubricant identified in this study, reduced sperm progressive motility to 49% of control after 10 minutes, even at concentrations as low as 5%. Vitality testing suggested the deterioration in progressive motility with Aquagel was not as a result of cell death. Conversely, Pré Vaginal Lubricant, a 'sperm-safe' lubricant, did not significantly affect any markers of sperm function assessed. Development of clinical guidance in this area is recommended to ensure HCPs deliver informed advice as lubricant use in couples trying-to-conceive may inadvertently contribute to delay in conception.


Assuntos
Fertilização , Lubrificantes/administração & dosagem , Lubrificação , Vagina , Feminino , Fertilidade , Pessoal de Saúde , Humanos , Lubrificantes/química , Lubrificação/métodos , Masculino , Concentração Osmolar , Vigilância em Saúde Pública , Análise do Sêmen , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia
18.
Indian J Med Res ; 149(1): 51-56, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31115375

RESUMO

Background & objectives: : Microsurgical reconstruction for idiopathic obstructive azoospermia is a challenging procedure, and selection of appropriate patients is important for successful outcomes. This prospective study was done to evaluate the ability of scrotal ultrasound measurements to predict the surgical feasibility and determine factors that could predict a patent anastomosis following vaso-epididymal anastomosis (VE) in men with idiopathic obstructive azoospermia. Methods: : In this prospective study, men diagnosed with idiopathic obstructive azoospermia, scheduled for a longitudinal intussusception VE, underwent a scrotal ultrasound measurement of testicular and epididymal dimensions. During surgery, site and type of anastomosis, presence of sperms in the epididymal fluid and technical satisfaction with the anastomosis were recorded. All men where VE could be performed were followed up for appearance of sperms in the ejaculate. Ultrasound parameters were compared between men who had a VE versus those with negative exploration. Predictive factors were compared between men with or without a patent anastomosis. Results: : Thirty four patients were included in the study conducted between September 2014 and August 2016 and a VE was possible in only 19 (55%) patients. Of these 19 patients, six had a patent anastomosis with one pregnancy. Preoperative ultrasound measurements could not identify patients where a VE could not be performed. Motile sperm in the epididymal fluid was the only significant predictor of a successful anastomosis. Interpretation & conclusion: : Forty five per cent of men planned for a VE for idiopathic obstructive azoospermia could not undergo a reconstruction. Ultrasound assessment of testicular and epididymal dimensions could not predict the feasibility of performing a VE. The presence of motile sperms in the epididymal fluid was the only significant predictor of a patent VE in our study.


Assuntos
Azoospermia/cirurgia , Epididimo/cirurgia , Infertilidade Masculina/cirurgia , Testículo/cirurgia , Adulto , Anastomose Cirúrgica/métodos , Azoospermia/fisiopatologia , Epididimo/fisiopatologia , Estudos de Viabilidade , Feminino , Humanos , Infertilidade Masculina/fisiopatologia , Masculino , Gravidez , Motilidade Espermática/fisiologia , Testículo/patologia
19.
J Trace Elem Med Biol ; 54: 142-149, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31109604

RESUMO

BACKGROUND: Metal ions are essential for numerous life processes. This study aims to investigate the relationship between seminal quality and ion levels in seminal plasma. BASIC PROCEDURES: A total of 205 semen samples were collected and seminal plasma ion levels were examined with inductively-coupled plasma-mass spectrometry. The nickel function was demonstrated by in vitro assay and cell growth. MAIN FINDINGS: The low sperm motility group showed distinctively reduced nickel concentration in seminal plasma compared with the normal sperm motility group. However, arsenic, sulfur, selenium, magnesium and zinc were negatively associated with sperm quality. No significant relationship between other examined cations and semen quality was observed. In vitro assay suggested low concentration of nickel significantly increased sperm total motility and progressive motility. Cell growth assay further confirmed nickel promoted eukaryotic yeast cell growth. Nickel level in seminal plasma may play important functions to determine sperm quality. PRINCIPAL CONCLUSIONS: Our study reveals a strong correlation between S, Mg, Se, Zn, As, Ni and seminal quality as well as discovers a novel functional role of nickel in sperm motility and eukaryotic cell growth. These findings may provide a potential avenue for assessment of sperm quality and treatment of reproduction disorders.


Assuntos
Níquel/farmacologia , Motilidade Espermática/efeitos dos fármacos , Células Cultivadas , Células Eucarióticas/efeitos dos fármacos , Células Eucarióticas/metabolismo , Humanos , Masculino , Níquel/metabolismo , Estresse Oxidativo/fisiologia , Selênio/metabolismo , Sêmen/química , Motilidade Espermática/fisiologia , Espermatozoides/efeitos dos fármacos , Espermatozoides/metabolismo , Oligoelementos/metabolismo , Zinco/metabolismo
20.
Theriogenology ; 132: 164-171, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31029847

RESUMO

Characteristics of frozen sperm associated with fertility and aging have not been fully determined in dogs. The aim of this study was to determine the relationship between fresh and post-thaw sperm quality, age, among the factors of motility, viability, morphology and oxidative stress in a group of fertile stud dogs with extensive breeding records and in dogs with reported subfertility problems. Sperm parameters from 39 fertile dogs were measured in fresh semen and frozen thawed semen. Additionally, frozen semen from 9 subfertile dogs was thawed and analyzed. Subfertile dogs were defined by referring veterinarians solely on the basis of owner history; breeding records were not available to this study. Evaluation included total motility (TM) and progressive motility (PM), average path velocity (VAP), viability, morphology and presence of sperm reactive oxygen species (ROS). Fertile males' ages ranged from 1 to 10 years, subfertile males' ages ranged from 4 to 14 years. All dogs were assigned to age groups according to age as young (1-3 years) middle (4-6 years) and senior (>7 years). The effect of sperm state (fresh vs. frozen-thawed), age, and fertility history (fertile vs. subfertile) on the measured endpoints were determined using a mixed effects model. TM showed a negative correlation with age in the frozen-thawed semen. Additionally, TM in fresh semen was higher in all age groups compared to post thaw semen (P < 0.05). PM was higher in all age dogs of fresh semen compared to frozen-thawed semen (P < 0.05). As such, TM and PM in post thaw semen statistically decreased compared to fresh semen regardless of age of stud male (P < 0.05). Differences in VAP were only observed between young and senior age groups in fresh semen (P < 0.05) while young and middle age dogs from fresh semen were different in all age groups for frozen-thawed semen (P < 0.05). ROS was higher in the young frozen-thawed semen compared to the young fresh semen (P < 0.05). When comparing TM, PM and VAP of fertile vs. subfertile dogs, middle aged fertile dogs are different from middle aged and senior subfertile dogs (P < 0.05). No differences were found in measures of ROS between fertile and subfertile. For sperm viability and morphology, differences were observed between all age groups of fertile dogs in comparison to all ages of subfertile dogs (P < 0.05). Sperm motility appeared to be the most affected parameter by freezing damage than any other parameter measured in this study, while we were not able to determine a significant association between ROS production and fertility status.


Assuntos
Envelhecimento , Criopreservação/veterinária , Cães , Preservação do Sêmen/veterinária , Sêmen/fisiologia , Animais , Fertilidade , Masculino , Espécies Reativas de Oxigênio , Motilidade Espermática/fisiologia , Espermatozoides/fisiologia
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