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1.
BMC Oral Health ; 24(1): 796, 2024 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-39010031

RESUMO

BACKGROUND: The assessment of hard and soft tissue at edentulous sites is important for subsequent implant treatment design. The aim of the present study was to explore the associations between the keratinized mucosa width (KMW) and the underlying alveolar bone dimensions at partial edentulous molar sites. METHODS: In this retrospective study, a total of 110 patients with at least one missing molar were selected. The buccal KMW of the edentulous molar sites was evaluated. Cone-beam computed tomography scans were collected, and the height discrepancy between the alveolar crest and the buccal bone plate (HC-B) as well as the alveolar bone height (ABH) were measured. The KMW was compared among the HC-B and ABH groups at both maxillary and mandibular sites. Linear regression and generalized estimation equations (GEEs) were used to explore the associations between the KMW and alveolar bone dimensions at α = 0.05. RESULTS: Among the 110 patients, 158 edentulous molar sites were analyzed. The average HC-B and ABH were significantly lower at the maxillary sites (1.26 ± 1.62 mm, 11.62 ± 3.94 mm) than at the mandibular sites (3.67 ± 2.85 mm, 14.91 ± 3.01 mm, p < 0.001). The KMW was significantly lower at sites with HC-B > 2 mm than at sites with HC-B ≤ 2 mm both in the maxilla and mandible (p < 0.001). No significant differences were found between the KMW at sites with ABH < 10 mm and sites with ABH ≥ 10 mm (p > 0.05). Linear regression and GEEs analyses revealed that the HC-B was significantly associated with the KMW (B = -0.339, p < 0.001), while the association between the KMW and the ABH was not statistically significant (B = -0.046, p = 0.352). CONCLUSIONS: The buccal KMW at edentulous molar sites was significantly associated with the HC-B. Alveolar ridges presenting with a sloped configuration were more prone to possess a narrower band of keratinized mucosa. Both hard and soft tissue augmentation should be considered for implant treatment at these sites. The correlations of dynamic changes between the KMW and alveolar bone dimensions after tooth extraction should be further investigated.


Assuntos
Processo Alveolar , Tomografia Computadorizada de Feixe Cônico , Dente Molar , Humanos , Estudos Retrospectivos , Feminino , Masculino , Processo Alveolar/diagnóstico por imagem , Processo Alveolar/patologia , Pessoa de Meia-Idade , Estudos Transversais , Dente Molar/diagnóstico por imagem , Arcada Parcialmente Edêntula/diagnóstico por imagem , Arcada Parcialmente Edêntula/patologia , Adulto , Idoso , Mucosa Bucal/diagnóstico por imagem , Mucosa Bucal/patologia , Mandíbula/diagnóstico por imagem , Mandíbula/patologia , Maxila/diagnóstico por imagem , Maxila/patologia
2.
BMC Oral Health ; 24(1): 755, 2024 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-38951844

RESUMO

BACKGROUND/PURPOSE: Oral cancer, including malignancies of the tongue, lips, floor of the mouth, cheek mucosa, gums, palate, and oropharynx, is life-threatening. Early diagnosis and appropriate treatment are crucial for long-term survival. Dentists frequently encounter oral cancers due to the nature of their work. This study aimed to evaluate the knowledge and experience of dentists in Turkey regarding oral cancers. MATERIALS AND METHODS: A total of 361 participants were included in the study, and survey questions were sent via email. The survey consisted of 16 questions measuring demographic data and knowledge about oral cancerous lesions. Participants were grouped based on their specialization and knowledge level, and differences in responses among groups were examined. RESULTS: Only 21.3% of the participants felt they had sufficient knowledge and experience about oral cancerous lesions. Overall, the correct answer rates indicated a moderate level of knowledge and experience. When grouped by specialization, oral surgeons had the highest accuracy in their responses (p < 0.05). CONCLUSION: Dentists are the professional group that most frequently encounters clinically oral cancerous lesions. Therefore, it is critically important for them to be knowledgeable and experienced to reduce morbidity and mortality through early diagnosis. This study evaluated the knowledge status of dentists in Turkey regarding oral cancer and highlighted the need for improved education.


Assuntos
Odontólogos , Neoplasias Bucais , Humanos , Turquia , Neoplasias Bucais/diagnóstico , Odontólogos/psicologia , Feminino , Masculino , Adulto , Pessoa de Meia-Idade , Conhecimentos, Atitudes e Prática em Saúde , Inquéritos e Questionários , Competência Clínica , Mucosa Bucal/patologia
3.
Technol Health Care ; 32(4): 2825-2836, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38995741

RESUMO

BACKGROUND: The radiation released at the time of dental panoramic radiographs causes genotoxic and cytotoxic effects on epithelial cells. OBJECTIVE: This research aimed to evaluate the changes in the frequencies of micronucleated cells in patients' buccal epithelial cells following dental panoramic radiography. METHODS: 74 patients were recruited for the study who were advised for panoramic radiographs. Using a wooden spatula, the buccal epithelial cells were scraped from both cheeks before to panoramic radiation exposure and ten days after the panoramic radiation exposure. Giemsa stain was used to stain the cells, and 500 cells were scored on a slide to determine the frequency of micronuclei. To determine the difference between the frequency of micronuclei before and after radiation exposure, a paired t-test was used in the statistical analysis. RESULTS: The proportion of micronuclei cells was 0.11% before radiation exposure and 0.57% following radiation exposure after 10 days. A statistically significant increase in the frequencies of micronuclei was noted after radiation exposure values. CONCLUSION: This study revealed the genotoxicity of epithelial cells with dental panoramic radiation exposure. It is advised to reduce the use of such radiographs and to use only when there is no other diagnostic tool that is helpful or when absolutely essential.


Assuntos
Células Epiteliais , Testes para Micronúcleos , Mucosa Bucal , Radiografia Panorâmica , Humanos , Radiografia Panorâmica/efeitos adversos , Mucosa Bucal/efeitos da radiação , Mucosa Bucal/diagnóstico por imagem , Mucosa Bucal/citologia , Masculino , Feminino , Células Epiteliais/efeitos da radiação , Adulto , Pessoa de Meia-Idade , Micronúcleos com Defeito Cromossômico/efeitos da radiação , Adulto Jovem
4.
Int J Mol Sci ; 25(13)2024 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-39000262

RESUMO

Radiotherapy in the head-and-neck area is one of the main curative treatment options. However, this comes at the cost of varying levels of normal tissue toxicity, affecting up to 80% of patients. Mucositis can cause pain, weight loss and treatment delays, leading to worse outcomes and a decreased quality of life. Therefore, there is an urgent need for an approach to predicting normal mucosal responses in patients prior to treatment. We here describe an assay to detect irradiation responses in healthy oral mucosa tissue. Mucosa specimens from the oral cavity were obtained after surgical resection, cut into thin slices, irradiated and cultured for three days. Seven samples were irradiated with X-ray, and three additional samples were irradiated with both X-ray and protons. Healthy oral mucosa tissue slices maintained normal morphology and viability for three days. We measured a dose-dependent response to X-ray irradiation and compared X-ray and proton irradiation in the same mucosa sample using standardized automated image analysis. Furthermore, increased levels of inflammation-inducing factors-major drivers of mucositis development-could be detected after irradiation. This model can be utilized for investigating mechanistic aspects of mucositis development and can be developed into an assay to predict radiation-induced toxicity in normal mucosa.


Assuntos
Mucosa Bucal , Humanos , Mucosa Bucal/efeitos da radiação , Raios X/efeitos adversos , Lesões por Radiação/etiologia , Lesões por Radiação/patologia , Masculino , Mucosite/etiologia , Mucosite/patologia , Feminino , Relação Dose-Resposta à Radiação , Estomatite/etiologia , Estomatite/patologia , Adulto , Pessoa de Meia-Idade
5.
J Oral Sci ; 66(3): 202-205, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39010170

RESUMO

The present work reports a case of a female patient complaining of itching and painful lesions affecting the oral mucosa for 7 months. Buccal and lip mucosa showed swelling and erythema, with serpiginous tracks. The patient was diagnosed with oral larva migrans, and the lesions resolved after ivermectin administration. At 18-month follow-up, no sign of recurrence was observed. Larva migrans can represent a pitfall in oral diagnosis and a stressful condition for the patient. Oral health care providers should be aware of this and keep this disease in mind as a possible differential diagnosis in oral mucosa lesions.


Assuntos
Larva Migrans , Humanos , Feminino , Larva Migrans/diagnóstico , Diagnóstico Diferencial , Doenças da Boca/diagnóstico , Doenças da Boca/parasitologia , Ivermectina/uso terapêutico , Adulto , Mucosa Bucal/patologia , Mucosa Bucal/parasitologia
6.
Clin Exp Dent Res ; 10(4): e932, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38973200

RESUMO

OBJECTIVES: There is a growing evidence to suggest augmenting peri-implant keratinized mucosa in the presence of ≤ 2 mm of keratinized mucosa. However, the most appropriate surgical technique and augmentation materials have yet to be defined. The aim of this systematic review and meta-analyses was to evaluate the clinical and patient-reported outcomes of augmenting keratinized mucosa around implants using free gingival graft (FGG) versus xenogeneic collagen matrix (XCM) before commencing prosthetic implant treatment. MATERIAL AND METHODS: Electronic databases were searched to identify observational studies comparing implant sites augmented with FGG to those augmented with XCM. The risk of bias was assessed using the Cochrane Collaboration's Risk of Bias tool. RESULTS: Six studies with 174 participants were included in the present review. Of these, 87 participants had FGG, whereas the remaining participants had XCM. At 6 months, sites augmented with FGG were associated with less changes in the gained width of peri-implant keratinized mucosa compared to those augmented with XCM (mean difference 1.06; 95% confidence interval -0.01 to 2.13; p = 0.05). The difference, however, was marginally significant. The difference between the two groups in changes in thickness of peri-implant keratinized mucosa at 6 months was statistically significantly in favor of FGG. On the other hand, XCM had significantly shorter surgical time, lower postoperative pain score, and higher color match compared to FGG. CONCLUSIONS: Within the limitation of this review, the augmentation of keratinized mucosa using FGG before the placement of the final prosthesis may have short-term positive effects on soft tissue thickness. XCM might be considered in aesthetically demanding implant sites and where patient comfort or shorter surgical time is a priority. The evidence support, however, is of low to moderate certainty; therefore, further studies are needed to support the findings of the present review.


Assuntos
Colágeno , Implantes Dentários , Gengiva , Humanos , Colágeno/uso terapêutico , Gengiva/transplante , Gengiva/patologia , Gengiva/cirurgia , Queratinas , Mucosa Bucal/transplante , Gengivoplastia/métodos , Implantação Dentária Endóssea/métodos , Xenoenxertos
7.
Zhonghua Bing Li Xue Za Zhi ; 53(7): 667-671, 2024 Jul 08.
Artigo em Chinês | MEDLINE | ID: mdl-38955696

RESUMO

Objective: To investigate the clinicopathological features and differential diagnosis of primary mucosal CD30-positive T-cell lymphoproliferative disorders (pmCD30+TLPD). Methods: Eight cases of pmCD30+TLPD diagnosed from 2013 to 2023 at the Department of Pathology, Beijing Friendship Hospital Affiliated to Capital Medical University and Beijing Ludaopei Hospital were retrospectively collected. The immunophenotype, EBV infection status and T-cell receptor (TCR) clonability of tumor cells were examined. The clinicopathological features were analyzed and related literatures were reviewed. Results: There were 5 females and 3 males, aged 28 to 73 years, without B symptoms, lack of trauma and autoimmune diseases. Seven cases occurred in oral mucosa and one in anal canal mucosa. Submucosal nodules with ulcerations were presented in all cases except one, which only submucosal nodule. Morphologically, there was different distribution of allotypic lymphocytes in inflammatory background. Four cases showed "kidney-shaped", "embryonic" and "horseshoe-shaped" cells, and one case resembled Hodgkin and Reed/Sternberg (HRS) cells. Allotypic lymphocytes expressed CD3 (7/8), CD4+/CD8-(7/8) and CD4-/CD8-(1/8). CD30 was uniformly strongly positive while ALK and CD56 were negative. In situ hybridization of EBER was negative in five cases (5/5). Clonal TCR gene rearrangement was positive in two cases. Four patients did not receive radiotherapy or chemotherapy. All the seven patients survived without disease except one died due to concurrent leukopenia. Conclusions: pmCD30+TLPD had a broad morphological spectrum and could be easily confused with primary cutaneous CD30+TLPD and systemic ALK-negative anaplastic large cell lymphoma involving mucosa, which may lead to misdiagnosis. Although the majority of the cases had a favorable prognosis, a few cases relapsed or progressed to lymphoma.


Assuntos
Antígeno Ki-1 , Transtornos Linfoproliferativos , Humanos , Masculino , Feminino , Idoso , Adulto , Transtornos Linfoproliferativos/patologia , Transtornos Linfoproliferativos/metabolismo , Antígeno Ki-1/metabolismo , Pessoa de Meia-Idade , Estudos Retrospectivos , Diagnóstico Diferencial , Linfócitos T/patologia , Linfócitos T/imunologia , Mucosa Bucal/patologia , Células de Reed-Sternberg/patologia , Células de Reed-Sternberg/metabolismo , Infecções por Vírus Epstein-Barr , Imunofenotipagem , Receptores de Antígenos de Linfócitos T/metabolismo , Receptores de Antígenos de Linfócitos T/genética
8.
Clin Exp Dent Res ; 10(4): e926, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38970232

RESUMO

OBJECTIVES: Electronic nicotine delivery systems (e-cigarette, pod, and vape) are currently among the tobacco consumption of adolescents and young adults. The aim is to show oral mucosa and saliva alterations related to vape. MATERIAL AND METHODS: A vape-user patient, presenting a white plaque in the posterior region of the hard palate, underwent clinical examination, sialometry, pH evaluation, and excisional biopsy of the white lesion. Molecular changes in saliva and vape liquid were analyzed by vibrational spectroscopy. RESULTS: The histopathological analyses showed hyperparakeratosis without dysplasia. Formaldehyde, ketones, and aromatic hydrocarbon species were identified in e-cig liquid by the FTIR. CONCLUSIONS: The use of vape may be related to the development of hyperkeratotic lesions in the oral mucosa as well as significantly modify the patient's salivary patterns as the vape liquid presents carcinogenic and cytotoxic components in its composition.


Assuntos
Mucosa Bucal , Saliva , Humanos , Saliva/química , Mucosa Bucal/patologia , Sistemas Eletrônicos de Liberação de Nicotina , Vaping/efeitos adversos , Masculino , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Adulto , Palato Duro/patologia , Adulto Jovem , Biópsia
9.
BMC Microbiol ; 24(1): 197, 2024 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-38849732

RESUMO

BACKGROUND: Emerging evidence emphasized the role of oral microbiome in oral lichen planus (OLP). To date, no dominant pathogenic bacteria have been identified consistently. It is noteworthy that a decreased abundance of Streptococcus, a member of lactic acid bacteria (LAB) in OLP patients has been commonly reported, indicating its possible effect on OLP. This study aims to investigate the composition of LAB genera in OLP patients by high-throughput sequencing, and to explore the possible relationship between them. METHODS: We collected saliva samples from patients with OLP (n = 21) and healthy controls (n = 22) and performed 16 S rRNA gene high-throughput sequencing. In addition, the abundance of LAB genera was comprehensively analyzed and compared between OLP and HC group. To verify the expression of Lactococcus lactis, real time PCR was conducted in buccal mucosa swab from another 14 patients with OLP and 10 HC. Furthermore, the correlation was conducted between clinical severity of OLP and LAB. RESULTS: OLP and HC groups showed similar community richness and diversity. The members of LAB, Lactococcus and Lactococcus lactis significantly decreased in saliva of OLP cases and negatively associated with OLP severity. In addition, Lactococcus and Lactococcus lactis showed negative relationship with Fusobacterium and Aggregatibacter, which were considered as potential pathogens of OLP. Similarly, compared with healthy controls, the amount of Lactococcus lactis in mucosa lesion of OLP patients was significantly decreased. CONCLUSIONS: A lower amount of Lactococcus at genus level, Lactococcus lactis at species level was observed in OLP cases and associated with disease severity. Further studies to verify the relationship between LAB and OLP, as well as to explore the precise mechanism is needed.


Assuntos
Lactobacillales , Líquen Plano Bucal , Microbiota , RNA Ribossômico 16S , Saliva , Humanos , Saliva/microbiologia , Feminino , Masculino , Líquen Plano Bucal/microbiologia , Pessoa de Meia-Idade , Lactobacillales/genética , Lactobacillales/isolamento & purificação , Lactobacillales/classificação , RNA Ribossômico 16S/genética , Adulto , Sequenciamento de Nucleotídeos em Larga Escala , Idoso , Mucosa Bucal/microbiologia , Estudos de Casos e Controles , DNA Bacteriano/genética , Lactococcus lactis/genética , Lactococcus lactis/isolamento & purificação
10.
F1000Res ; 13: 152, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38854440

RESUMO

Background: Managing recalcitrant oral lichen planus (OLP) can be challenging. Laser therapy has been suggested as an alternative to corticosteroids for treatment. Photodynamic therapy (PDT) is a non-invasive technique that enables the removal of lesions without surgery. Photobiomodulation therapy (PBMT) can promote healing and recovery of the lesions. Case presentation: The objective was to treat unresponsive bilateral OLP of the whole buccal mucosae with a combination of PDT and PBMT. Results: A 43-year-old Thai male presented with the severe painful reticular type of OLP of bilateral buccal mucosae involving upper and lower vestibular areas. The lesions were not remitted with either prednisolone systemic steroids or fluocinolone topical corticosteroids. After undergoing ten sessions of PDT with 10% 5-Aminolevulinic acid (5-ALA) in the form of thermoplastic gel and a 635 nm diode laser at 100 to 400 mW with an energy density of 20 to 30 J/cm 2 in continuous wave mode, combined with five interim-sessions of PBMT using a 635 nm diode laser at 200 to 300 mW with an energy density of 6 to 10 J/cm 2 in continuous wave, the patient reported relief of burning sensation beside remission of lesions without any complications. Conclusion: The wide-spreading recalcitrant OLP with burning sensation can be managed by combining PDT and PBMT.


Assuntos
Líquen Plano Bucal , Terapia com Luz de Baixa Intensidade , Mucosa Bucal , Fotoquimioterapia , Humanos , Masculino , Adulto , Fotoquimioterapia/métodos , Líquen Plano Bucal/tratamento farmacológico , Líquen Plano Bucal/terapia , Líquen Plano Bucal/radioterapia , Líquen Plano Bucal/patologia , Mucosa Bucal/patologia , Terapia com Luz de Baixa Intensidade/métodos , Terapia Combinada , Ácido Aminolevulínico/uso terapêutico , Ácido Aminolevulínico/análogos & derivados
11.
Swiss Dent J ; 134(3)2024 Jun 07.
Artigo em Alemão | MEDLINE | ID: mdl-38847055

RESUMO

The oral verruciform xanthoma (OVX) is a rare, benign lesion that occurs predominantly in the masticatory region of the oral cavity. The OVX is small, slow growing, and mostly free of clinical symptoms. The exact pathogenesis is unknown, and a viral etiology such as from a human papillomavirus (HPV) infection has not been proven. Although primarily observed in healthy individuals, there have been cases in patients with autoimmune diseases and with chronic graft-versus-host disease (GvHD). The treatment of choice is complete excision of the lesion. This case report showcases a successful surgical removal of an oral verruciform xanthoma on the left buccal mucosa in a 56-year-old patient with GvHD 14 years after allo-genic stem cell transplantation due to a Non-Hodgkin lymphoma.


Assuntos
Bochecha , Doença Enxerto-Hospedeiro , Xantomatose , Humanos , Pessoa de Meia-Idade , Xantomatose/diagnóstico , Xantomatose/cirurgia , Doença Enxerto-Hospedeiro/diagnóstico , Doenças da Boca/diagnóstico , Doenças da Boca/cirurgia , Doenças da Boca/terapia , Masculino , Mucosa Bucal/patologia , Diagnóstico Diferencial
12.
Curr Opin Otolaryngol Head Neck Surg ; 32(4): 239-247, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38837190

RESUMO

PURPOSE OF REVIEW: This review aims to examine the indications and anatomical circumstances for when to optimally incorporate buccal myomucosal flaps (BMFs) into palatal surgical reconstruction. RECENT FINDINGS: Studies examining outcomes following primary cleft palate repair with incorporation of BMF have demonstrated excellent speech outcomes and low rates of fistula. Furthermore, some reports cite an association of buccal flap use with reduced midface hypoplasia and the need for later orthognathic surgery. When used for secondary speech surgery, BMFs have been shown to lead to speech improvements across multiple outcome measures. Advantages of BMF techniques over conventionally described pharyngeal flap and pharyngoplasty procedures include significant lengthening of the velum, favorable repositioning of the levator muscular sling, and lower rates of obstructive sleep apnea. SUMMARY: Although the published data demonstrate excellent outcomes with use of BMFs for primary and secondary palatal surgery, there are limited data to conclude superiority over the traditional, more extensively investigated surgical techniques. The authors of this review agree with the evidence that BMF techniques can be useful in primary palatoplasty for congenitally wide clefts, secondary speech surgery for large velopharyngeal gaps, and/or in individuals with a predisposition for airway obstruction from traditional approaches.


Assuntos
Fissura Palatina , Procedimentos de Cirurgia Plástica , Retalhos Cirúrgicos , Insuficiência Velofaríngea , Humanos , Insuficiência Velofaríngea/cirurgia , Insuficiência Velofaríngea/etiologia , Fissura Palatina/cirurgia , Procedimentos de Cirurgia Plástica/métodos , Mucosa Bucal/transplante
13.
Biomed Mater ; 19(5)2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-38917837

RESUMO

Insufficient osseointegration of titanium-based implants is a factor conditioning their long-term success. Therefore, different surface modifications, such as multifunctional oxide coatings, calcium phosphates, and the addition of molecules such as peptides, have been developed to improve the bioactivity of titanium-based biomaterials. In this work, we investigate the behavior of human oral mucosal stem cells (hOMSCs) cultured on amorphous titanium oxide (aTiO2), surfaces designed to simulate titanium (Ti) surfaces, biofunctionalized with a novel sequence derived from cementum attachment protein (CAP-p15), exploring its impact on guiding hOMSCs towards an osteogenic phenotype. We carried out cell attachment and viability assays. Next, hOMSCs differentiation was assessed by red alizarin stain, ALP activity, and western blot analysis by evaluating the expression of RUNX2, BSP, BMP2, and OCN at the protein level. Our results showed that functionalized surfaces with CAP-p15 (1 µg ml-1) displayed a synergistic effect increasing cell proliferation and cell attachment, ALP activity, and expression of osteogenic-related markers. These data demonstrate that CAP-p15 and its interaction with aTiO2surfaces promote osteoblastic differentiation and enhanced mineralization of hOMSCs when compared to pristine samples. Therefore, CAP-p15 shows the potential to be used as a therapeutical molecule capable of inducing mineralized tissue regeneration onto titanium-based implants.


Assuntos
Adesão Celular , Diferenciação Celular , Proliferação de Células , Mucosa Bucal , Osteogênese , Células-Tronco , Titânio , Titânio/química , Humanos , Osteogênese/efeitos dos fármacos , Mucosa Bucal/citologia , Mucosa Bucal/metabolismo , Células-Tronco/citologia , Células-Tronco/metabolismo , Propriedades de Superfície , Células Cultivadas , Osteoblastos/citologia , Osteoblastos/metabolismo , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Sobrevivência Celular , Osseointegração/efeitos dos fármacos , Materiais Biocompatíveis/química
14.
Zhongguo Ying Yong Sheng Li Xue Za Zhi ; 40: e20240003, 2024 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-38925868

RESUMO

The buccal route has great prospects and possible benefits for the administration of drugs systemically. The present study involves designing, developing and optimising the buccal tablet formulation of Enalapril Maleate (EM) by using the QbD approach. We prepared the EM buccal tablets using the dry granulation method. In the QTPP profile, the CQAs for EM buccal tablets are Mucoadhesive strength, swelling index and drug release (dependent variables); the CMAs identified for EM buccal tablets were Carbopol 934P, HPMC-K100M and chitosan (independent variables). Diluent quantity, blending time and compression force were selected as CPPs; the Box-Behnkentdesign was used to evaluate the relationship between the CMAs and CPPs. Based on the DoE, the composition of the optimised formulation of EM BT-18 consists of 20mg of EM, 15 mg of carbopol 934p, 17 mg of HPMC-K100M, 10mg of chitosan, 30 mg of PVP K-30, 1 mg of magnesium stearate, 16 mg of Mannitol, 1 mg of aspartame, and 50 mg of Ethyl cellulose. The optimised formulation of EM BT 18 was found to have a Mucoadhesive strength of 24.32±0.30g. The swelling index was 90.74±0.25% and drug release was sustained up to 10 hours 98.4±3.62% compared to the marketed product, whose release was up to 8 hours. We attempted to design a buccal tablet of Enalapril Maleate for sustained drug release in the treatment of hypertension. Patients who cannot take oral medication due to trauma or unconscious conditions could receive the formulation. Development of a newly P.ceutical product is very time-consuming, extremely costly and high-risk, with very little chance of a successful outcome. Hence, this study showed EM tablets are already available on the market but we have chosen a buccal drug delivery system using a novel approach using QbD tools to target the quality of the product accurately.


Assuntos
Enalapril , Comprimidos , Enalapril/química , Enalapril/administração & dosagem , Administração Bucal , Mucosa Bucal , Composição de Medicamentos , Química Farmacêutica/métodos
15.
J Appl Oral Sci ; 32: e20240047, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38922243

RESUMO

OBJECTIVE: To assess the efficacy of Phyllanthus emblica extract in alleviating halitosis and reducing the inflammatory response to halitosis-related bacteria. METHODOLOGY: This investigation, using Phyllanthus emblica fruit extract (PE), involved four aspects. First, we evaluated the effect on growth and aggregation of halitosis-related bacteria, including Fusobacterium nucleatum, Porphyromonas gingivalis, and Solobacterium moorei, using a microdilution assay and scanning electron microscopy. Second, volatile sulfur compound (VSC) levels were measured on individuals with halitosis in randomized short-term (26 participants) and double-blind randomized long-term trials (18 participants in each group) after rinsing with PE for 3, 6, and 12 h, and 28 days. Third, we analyzed pro-inflammatory cytokine expression in TR146 cells using quantitative real-time PCR and enzyme-linked immunosorbent assays. Lastly, we assessed pro-inflammatory cytokine secretion and Toll-like receptor (TLR) 2 mRNA expression via the same experimental methods in a three-dimensional oral mucosal epithelial model (3D OMEM). RESULTS: PE extract dose-dependently inhibited the growth of F. nucleatum (50% inhibition concentration [IC50]=0.079%), P. gingivalis (IC50=0.65%), and S. moorei (IC50=0.07%) and effectively prevented bacterial aggregation. Furthermore, VSC contents decreased significantly at 3, 6, and 12 h after rinsing with 5% PE compared with those in the control. Long-term use of mouthwash containing 5% PE for 28 days led to a significant decrease in VSC contents. PE attenuated the F. nucleatum- or P. gingivalis-stimulated mRNA expression and protein release of interleukin (IL)-6 and IL-8 in TR146 cells. It also suppressed IL-8 and prostaglandin E2 secretion and TLR2 mRNA expression in F. nucleatum-induced OMEMs. CONCLUSION: Our findings support the use of PE in oral care products to alleviate halitosis and it may reduce inflammation.


Assuntos
Citocinas , Ensaio de Imunoadsorção Enzimática , Fusobacterium nucleatum , Halitose , Microscopia Eletrônica de Varredura , Phyllanthus emblica , Extratos Vegetais , Porphyromonas gingivalis , Reação em Cadeia da Polimerase em Tempo Real , Phyllanthus emblica/química , Halitose/tratamento farmacológico , Halitose/microbiologia , Humanos , Extratos Vegetais/farmacologia , Método Duplo-Cego , Fusobacterium nucleatum/efeitos dos fármacos , Porphyromonas gingivalis/efeitos dos fármacos , Feminino , Fatores de Tempo , Masculino , Resultado do Tratamento , Adulto , Adulto Jovem , Receptor 2 Toll-Like/efeitos dos fármacos , Frutas/química , Estatísticas não Paramétricas , Mucosa Bucal/efeitos dos fármacos , Mucosa Bucal/microbiologia , Análise de Variância , Compostos de Enxofre/farmacologia , Compostos de Enxofre/análise
16.
Artigo em Inglês | MEDLINE | ID: mdl-38865574

RESUMO

Women living with human immunodeficiency virus are at an increased risk of developing cancers related to human papillomavirus (HPV). Thus, it is important to combine clinical assessments, serological screening, and HPV data for planning prevention policies. This study aimed to identify HPV and its specific types in the cervical, anal, and oral mucosa of HIV-seropositive women, associating it with viral load and lymphocyte count. Sociodemographic characteristics, health data (CD4+ and CD8+ T cell counts and viral load), and biological samples (cervical, anal, and oral) were collected from 86 HIV-positive women undergoing antiretroviral therapy. Data were classified according to the presence or absence of HPV-DNA, HPV-DNA presence at one or more anatomic sites, and level of oncogenic risk, considering low- and high-risk oncogenic HPV-DNA groups. The presence of HPV in the cervicovaginal site was 65.9%, 63.8% in anal canal, and 4.2% in oral mucosa. A viral load ≥75 HIV copies/mL was associated with the presence of HPV-DNA. There was an association between viral load and the low-risk HPV or high-risk HPV groups. We found a high prevalence of HPV infection in HIV-seropositive women, particularly in the cervical and anal mucosa, with viral load ≥75 HIV copies/mL being associated with HPV-DNA presence.


Assuntos
Colo do Útero , DNA Viral , Infecções por HIV , Infecções por Papillomavirus , Carga Viral , Humanos , Feminino , Infecções por Papillomavirus/complicações , Adulto , Infecções por HIV/complicações , Infecções por HIV/virologia , DNA Viral/análise , Colo do Útero/virologia , Papillomaviridae/genética , Papillomaviridae/isolamento & purificação , Pessoa de Meia-Idade , Contagem de Linfócitos , Mucosa Bucal/virologia , Canal Anal/virologia , Prevalência , Estudos Transversais , Fatores Socioeconômicos , Contagem de Linfócito CD4 , Fatores de Risco , Papillomavirus Humano
17.
Cells ; 13(11)2024 Jun 06.
Artigo em Inglês | MEDLINE | ID: mdl-38891124

RESUMO

Canine oral melanoma is the most prevalent malignant tumor in dogs and has a poor prognosis due to its high aggressiveness and high metastasis and recurrence rates. More research is needed into its treatment and to understand its pathogenic factors. In this study, we isolated a canine oral mucosal melanoma (COMM) cell line designated as COMM6605, which has now been stably passaged for more than 100 generations, with a successful monoclonal assay and a cell multiplication time of 22.2 h. G-banded karyotype analysis of the COMM6605 cell line revealed an abnormal chromosome count ranging from 45 to 74, with the identification of a double-armed chromosome as the characteristic marker chromosome of this cell line. The oral intralingual and dorsal subcutaneous implantation models of BALB/c-nu mice were successfully established; Melan-A (MLANA), S100 beta protein (S100ß), PNL2, tyrosinase-related protein 1 (TRP1), and tyrosinase-related protein 2 (TRP2) were stably expressed positively in the canine oral tumor sections, tumor cell lines, and tumor sections of tumor-bearing mice. Sublines COMM6605-Luc-EGFP and COMM6605-Cherry were established through lentiviral transfection, with COMM6605-Luc-EGFP co-expressing firefly luciferase (Luc) and enhanced green fluorescent protein (EGFP) and COMM6605-Cherry expressing the Cherry fluorescent protein gene. The COMM6605-Luc-EGFP fluorescent cell subline was injected via the tail vein and caused lung and lymph node metastasis, as detected by mouse live imaging, which can be used as an animal model to simulate the latter steps of hematogenous spread during tumor metastasis. The canine oral melanoma cell line COMM6605 and two sublines isolated and characterized in this study can offer a valuable model for studying mucosal melanoma.


Assuntos
Melanoma , Mucosa Bucal , Neoplasias Bucais , Animais , Cães , Melanoma/patologia , Melanoma/genética , Melanoma/veterinária , Neoplasias Bucais/patologia , Neoplasias Bucais/genética , Neoplasias Bucais/veterinária , Linhagem Celular Tumoral , Mucosa Bucal/patologia , Mucosa Bucal/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Modelos Animais de Doenças , Camundongos Nus
18.
Lasers Med Sci ; 39(1): 152, 2024 Jun 08.
Artigo em Inglês | MEDLINE | ID: mdl-38849656

RESUMO

In clinical practice, an innovative laser technology that provides contactless preparation of soft tissues with a wavelength of 445 nm has been introduced. This study aimed to investigate the morphological changes in the oral mucosa when exposed to laser radiation at a wavelength of 445 nm in the ablation mode.An experimental study was conducted to analyze the dynamics of reparative regeneration in the wound caused by that particular type of radiation, utilizing the procedure of lower lip frenuloplasty as an illustration. 48 sexually mature male laboratory rats were chosen as the research object. The procedure of preparing the oral vestibule was executed by employing a contactless laser beam with a wavelength of 445 nm and a power of 0.7 W in continuous mode (CW) and an uninitiated fiber.Histological examination showed that 25 min after the surgery, there were large areas of coagulation necrosis in the oral mucosa in the area affected by the blue laser. In 48 h, the area of necrosis decreased both in size and depth. By the 7th day after the surgery, the necrotic masses had grown into the connective tissue, while marginal regeneration of the epithelium was noted. By the 14th day, the wound surface was completely epithelialized, represented by fibrous scar tissue. Clinically, around the mandibular incisors, there was a wide area of attached keratinized gingiva.The findings of histological examination indicate a necrosis of coagulation type in the region of tissue ablation and also show the absence of phase II of the inflammatory response (the stage of exudation), which expedites the process of epithelialization of the oral mucosa wound.


Assuntos
Mucosa Bucal , Regeneração , Cicatrização , Animais , Mucosa Bucal/efeitos da radiação , Mucosa Bucal/cirurgia , Mucosa Bucal/patologia , Masculino , Projetos Piloto , Ratos , Cicatrização/efeitos da radiação , Regeneração/efeitos da radiação , Terapia a Laser/métodos , Terapia a Laser/instrumentação , Necrose
19.
Sci Rep ; 14(1): 13066, 2024 06 06.
Artigo em Inglês | MEDLINE | ID: mdl-38844764

RESUMO

The aim of this study was to assess the surface and tissue quality of keratinized mucosa grafts (KMG) obtained using the conventional scalpel and mucotome techniques. This was an experimental in vitro/ex vivo study involving six porcine hemi-mandibles. Specimens were harvested using both the mucotome and conventional scalpel techniques, with randomization determining the choice of technique for tissue removal. The specimens were prepared following predefined laboratory protocols and subsequently subjected to optical microscopy for evaluating epithelial and connective tissue and scanning electron microscopy for topographical and 3D profilometry analysis. Tissues harvested using the mucotome exhibited a linear base and uniform thickness, along with the presence of submucosa and fibrous connective tissue, all of which are ideal for graft success. Differences in the surface characteristics of specimens obtained through the two techniques were observed during a comparative analysis of images obtained through both microscopy types. KMG obtained using the mucotome technique displayed greater uniformity and reduced undesirable cell presence compared to the scalpel technique, thereby enhancing the likelihood of success in soft tissue graft surgical procedures. This study provides valuable insights to oral healthcare professionals and may contribute to future research aimed at achieving more successful surgeries, shorter postoperative recovery times, reduced discomfort, and an overall more positive patient experience.


Assuntos
Mandíbula , Mucosa Bucal , Animais , Suínos , Mucosa Bucal/transplante , Mucosa Bucal/citologia , Mandíbula/cirurgia , Queratinas/metabolismo , Microscopia Eletrônica de Varredura , Coleta de Tecidos e Órgãos/métodos
20.
Medicine (Baltimore) ; 103(23): e38401, 2024 Jun 07.
Artigo em Inglês | MEDLINE | ID: mdl-38847720

RESUMO

Gender identification plays a pivotal role in forensic medicine. Among the various methods used for gender identification, deoxyribose nucleic acid (DNA) based methods are considered accurate. Exfoliated oral mucosal cells that are harvested from oral hygiene aids can be potentially used for gender identification using real-time polymerase chain rection (PCR). The aim of the present longitudinal study is to assess and compare the efficacy of toothbrush and miswak as potential tools to harvest exfoliated cells for gender identification. Forty healthy volunteers were recruited and asked to clean their teeth using new toothbrush and fresh miswak each day for 4 days. Toothbrush and miswak used by the participants were subjected to DNA analysis immediately, 1st, 2nd and 6th month. The absorbance of DNA samples were quantified and gender identification was done by amplification of sex determining gene-Sex determining region Y gene (SRY) and ALT1 genes using real-time PCR. The number of correct and positive identification for samples at various time points were tabulated and subjected to statistical analysis. Post hoc power analysis showed that the study had a power of 93%. Correct and positive gender identification was observed for the samples (100%) obtained using miswak, for tooth brush it reduced to 95%, 80%, and 35% at the end of 1st, 2nd, and 6th month. The differences seen at the end of 2nd month and 6th month were statistically significant. Miswak is a better tool to harvest exfoliated cells for gender identification when compared to a toothbrush. Hence, miswak can serve as a potential tool in forensic medicine for DNA extraction and subsequently victim identification.


Assuntos
Reação em Cadeia da Polimerase em Tempo Real , Escovação Dentária , Humanos , Feminino , Masculino , Estudos Longitudinais , Escovação Dentária/instrumentação , Adulto , Reação em Cadeia da Polimerase em Tempo Real/métodos , Análise para Determinação do Sexo/métodos , Adulto Jovem , Mucosa Bucal/citologia , DNA/análise , Voluntários Saudáveis
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