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1.
J Chem Ecol ; 47(8-9): 755-767, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34463893

RESUMO

Banana plants are affected by various viral diseases, among which the most devastating is the "bunchy top", caused by the Banana bunchy top virus (BBTV) and transmitted by the aphid Pentalonia nigronervosa Coquerel. The effect of BBTV on attraction mechanisms of dessert and plantain banana plants on the vector remains far from elucidated. For that, attractiveness tests were carried out using a two columns olfactometer for apterous aphids, and a flight cage experiment for alate aphids. Volatile Organic Compounds (VOCs) emitted by either healthy or BBTV-infected banana plants were identified using a dynamic extraction system and gas-chromatography mass-spectrometry (GC-MS) analysis. Behavioral results revealed a stronger attraction of aphids towards infected banana plants (independently from the variety), and towards the plantain variety (independently from the infection status). GC-MS results revealed that infected banana plants produced VOCs of the same mixture as healthy banana plants but in much higher quantities. In addition, VOCs produced by dessert and plantain banana plants were different in nature, and plantains produced higher quantities than dessert banana trees. This work opens interesting opportunities for biological control of P. nigronervosa, for example by luring away the aphid from banana plants through manipulation of olfactory cues.


Assuntos
Afídeos/fisiologia , Babuvirus/fisiologia , Musa/química , Compostos Orgânicos Voláteis/análise , Animais , Comportamento Animal/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Controle de Insetos , Musa/crescimento & desenvolvimento , Musa/virologia , Doenças das Plantas/virologia , Análise de Componente Principal , Compostos Orgânicos Voláteis/farmacologia
2.
Sci Rep ; 11(1): 14592, 2021 07 16.
Artigo em Inglês | MEDLINE | ID: mdl-34272422

RESUMO

Banana, one of the most important staple fruit among global consumers is highly sterile owing to natural parthenocarpy. Identification of genetic factors responsible for parthenocarpy would facilitate the conventional breeders to improve the seeded accessions. We have constructed Protein-protein interaction (PPI) network through mining differentially expressed genes and the genes used for transgenic studies with respect to parthenocarpy. Based on the topological and pathway enrichment analysis of proteins in PPI network, 12 candidate genes were shortlisted. By further validating these candidate genes in seeded and seedless accession of Musa spp. we put forward MaAGL8, MaMADS16, MaGH3.8, MaMADS29, MaRGA1, MaEXPA1, MaGID1C, MaHK2 and MaBAM1 as possible target genes in the study of natural parthenocarpy. In contrary, expression profile of MaACLB-2 and MaZEP is anticipated to highlight the difference in artificially induced and natural parthenocarpy. By exploring the PPI of validated genes from the network, we postulated a putative pathway that bring insights into the significance of cytokinin mediated CLAVATA(CLV)-WUSHEL(WUS) signaling pathway in addition to gibberellin mediated auxin signaling in parthenocarpy. Our analysis is the first attempt to identify candidate genes and to hypothesize a putative mechanism that bridges the gaps in understanding natural parthenocarpy through PPI network.


Assuntos
Frutas/genética , Musa/genética , Partenogênese , Proteínas de Plantas/genética , Mapas de Interação de Proteínas , Transdução de Sinais , Citocininas/metabolismo , Frutas/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Giberelinas/metabolismo , Ácidos Indolacéticos/metabolismo , Musa/crescimento & desenvolvimento , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Sementes/genética
3.
PLoS One ; 16(7): e0253366, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34237070

RESUMO

The goal of this work was to evaluate changes in dietary fiber measured by the traditional enzymatic-gravimetric method (AOAC 991.43) and the more recently accepted modified enzymatic-gravimetric method (AOAC 2011.25), mono- and disaccharides, and starch as a function of assessed ripeness in a controlled study of a single lot of bananas and in bananas at the same assessed stages of ripeness from bananas purchased in retail stores, from different suppliers. Sugars, starch, and dietary fiber were analyzed in bananas from a single lot, at different stages of ripeness, and in retail samples at the same assessed stages of ripeness. Mean fiber measured by the traditional enzymatic-gravimetric method (EG) was ~2 g/100g and not affected by ripeness. Mean fiber assessed with the recently modified method (mEG) was ~18 g/100g in unripe fruit and decreased to 4-5 g/100g in ripe and ~2 g/100g in overripe bananas. Slightly ripe and ripe bananas differed by ~1.1 g/100g in the controlled single-lot study but not among retail samples. There was a large increase in fructose, glucose and total sugar going from unripe to ripe with no differences between ripe and overripe. Aside from stage of ripeness, the carbohydrate composition in retail bananas is likely affected by differences in cultivar and post-harvest handling. Results from this study demonstrate the importance of measuring dietary fiber using the mEG approach, developing more comprehensive and sensitive carbohydrate analytical protocols and food composition data, and recognizing the impact of different stages of maturity and ripeness on carbohydrate intake estimated from food composition data.


Assuntos
Fibras na Dieta/análise , Musa/química , Amido/análise , Açúcares/análise , Frutose/análise , Glucose/análise , Musa/crescimento & desenvolvimento , Supermercados
4.
Plant Signal Behav ; 16(5): 1884782, 2021 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-33793381

RESUMO

Banana as an important economic crop worldwide, often suffers from serious damage caused by Fusarium oxysporum f. sp. Cubense. Arbuscular mycorrhizal (AM) fungi have been considered as one of the promising plant biocontrol agents in preventing from root pathogens. This study examined the effect of AM fungal inoculation on plant growth and differential expressions of growth- and defense-related genes in banana seedlings. Tissue-cultured seedlings of Brazilian banana (Musa acuminate Cavendish cv. Brail) were inoculated with AM fungus (Rhizophagus irregularis, Ri), and developed good mycorrhizal symbiosis from 4 to 11 weeks after inoculation with an infection rate up to 71.7% of the roots system. Microbial abundance revealed that Ri abundance in banana roots was 1.85×106 copies/ml at 11 weeks after inoculaiton. Inoculation improved plant dry weights by 47.5, 124, and 129% for stem, leaf, and the whole plant, respectively, during phosphate depletion. Among a total of 1411 differentially expressed genes (DEGs) obtained from the transcriptome data analysis, genes related to plant resistance (e.g. POD, PAL, PYR, and HBP-1b) and those related to plant growth (e.g. IAA, GH3, SAUR, and ARR8) were up-regulated in AM plants. This study demonstrates that AM fungus effectively promoted the growth of banana plants and induced defense-related genes which could help suppress wilt disease. The outcomes of this study form a basis for further study on the mechanism of banana disease resistance induced by AM fungi.


Assuntos
Regulação da Expressão Gênica de Plantas , Musa/imunologia , Musa/microbiologia , Micorrizas/fisiologia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Plântula/genética , Plântula/microbiologia , Biomassa , Suscetibilidade a Doenças , Ontologia Genética , Musa/genética , Musa/crescimento & desenvolvimento , Metabolismo Secundário/genética , Transdução de Sinais/genética , Transcriptoma/genética
5.
BMC Plant Biol ; 21(1): 125, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33648452

RESUMO

BACKGROUND: Banana plant height is an important trait for horticultural practices and semi-dwarf cultivars show better resistance to damages by wind and rain. However, the molecular mechanisms controlling the pseudostem height remain poorly understood. Herein, we studied the molecular changes in the pseudostem of a semi-dwarf banana mutant Aifen No. 1 (Musa spp. Pisang Awak sub-group ABB) as compared to its wild-type dwarf cultivar using a combined transcriptome and metabolome approach. RESULTS: A total of 127 differentially expressed genes and 48 differentially accumulated metabolites were detected between the mutant and its wild type. Metabolites belonging to amino acid and its derivatives, flavonoids, lignans, coumarins, organic acids, and phenolic acids were up-regulated in the mutant. The transcriptome analysis showed the differential regulation of genes related to the gibberellin pathway, auxin transport, cell elongation, and cell wall modification. Based on the regulation of gibberellin and associated pathway-related genes, we discussed the involvement of gibberellins in pseudostem elongation in the mutant banana. Genes and metabolites associated with cell wall were explored and their involvement in cell extension is discussed. CONCLUSIONS: The results suggest that gibberellins and associated pathways are possibly developing the observed semi-dwarf pseudostem phenotype together with cell elongation and cell wall modification. The findings increase the understanding of the mechanisms underlying banana stem height and provide new clues for further dissection of specific gene functions.


Assuntos
Musa/crescimento & desenvolvimento , Musa/genética , Caules de Planta/crescimento & desenvolvimento , Caules de Planta/genética , Parede Celular/genética , Parede Celular/metabolismo , Giberelinas/metabolismo , Metaboloma , Fenótipo , Reguladores de Crescimento de Plantas/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Transcriptoma
6.
Microbiol Res ; 248: 126750, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33765636

RESUMO

The association of different species of endophytic bacteria with the rhizosphere of the host plants can stimulate growth, development and acclimatization, offering a greater quantity of seedlings, in addition to reducing the cycle, providing economic return to the producer. The objective of this study was to evaluate the effect of introduction four bacterial isolates through inoculation into the root system in three banana cultivars (Prata Anã, Grande Naine and BRS Princesa) in the acclimatization phase. The evaluated treatments were: control (nutrient broth without bacteria); Bacillus cereus strain 1 (BC1); Bacillus cereus strain 2 (BC2); Bacillus thuringiensis (BT); Buttiauxella agrestis (BA). The morphological characteristics related to the development of the plants (total height and pseudostem diameter) were evaluated throughout the acclimatization period. After 90 days of transplanting and acclimatization, root length, leaf number, dry root weight, pseudostem and leaf, leaf area, internal carbon concentration, stomatal conductance, photosynthesis rate, transpiration rate, leaf temperature and chlorophyll were evaluated. The bacteria showed different results in relation to the studied cultivars. Considering the morphological and physiological characteristics observed in this study, B. thuringiensis for the cultivars Prata Anã and Grande Naine and the B. agrestis for the cultivar BRS Princesa are recommended for the process of acclimatization of banana seedlings, as they stimulated growth of the plant, increasing the dry mass, besides promoting the growth of roots. In this way, they improved the physiological aspects of the plants and reduced the period of acclimatization of the banana.


Assuntos
Bacillus/fisiologia , Endófitos/fisiologia , Enterobacteriaceae/fisiologia , Musa/microbiologia , Musa/fisiologia , Adaptação Fisiológica , Inoculantes Agrícolas/fisiologia , Clorofila/metabolismo , Musa/crescimento & desenvolvimento , Fotossíntese , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/microbiologia , Raízes de Plantas/fisiologia , Plântula/crescimento & desenvolvimento , Plântula/microbiologia , Plântula/fisiologia
7.
BMC Plant Biol ; 21(1): 97, 2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33596830

RESUMO

BACKGROUND: Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana. RESULTS: In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. 'Dajiao'; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expression level of MaICE1 was significantly decreased in MaMAPK3 RNAi transgenic plants. Over-expression of MaICE1 in Cavendish banana (Musa spp. AAA group) indicated that the cold resistance of transgenic plants was superior to that of the WT plants. The POD P7 gene was significantly up-regulated in MaICE1-overexpressing transgenic plants compared with WT plants, and the POD P7 was proved to interact with MaICE1. CONCLUSIONS: Taken together, our work provided new and solid evidence that MaMAPK3-MaICE1-MaPOD P7 pathway positively improved the cold tolerance in monocotyledon banana, shedding light on molecular breeding for the cold-tolerant banana or other agricultural species.


Assuntos
Regulação da Expressão Gênica de Plantas , Proteína Quinase 3 Ativada por Mitógeno/metabolismo , Musa/fisiologia , Proteínas de Plantas/metabolismo , Fatores de Transcrição/metabolismo , Temperatura Baixa , Resposta ao Choque Frio , Proteína Quinase 3 Ativada por Mitógeno/genética , Musa/genética , Musa/crescimento & desenvolvimento , Folhas de Planta/genética , Folhas de Planta/fisiologia , Proteínas de Plantas/genética , Fatores de Transcrição/genética
8.
Mol Biol Rep ; 48(2): 1127-1138, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33492573

RESUMO

The BAHD family is involved in different biological roles in plants, including secondary metabolite synthesis, improving abiotic/biotic stress resistance, and influencing fruit quality. However, the knowledge about BAHD in banana, an important fruit crop, is limited. In this study, 46 banana BAHD genes (MaBAHDs) were identified and divided into four groups according to phylogenetic analysis. Most of the MaBAHD genes in the same group presented similar conserved motifs and genetic structures. MaBAHD genes have similar expression patterns in two banana varieties, and more genes showed high expressions in the roots. The comprehensive MaBAHD gene expression patterns obtained from two varieties of banana showed valuable information regarding their participation in fruit development, ripening, and response to abiotic/biotic stresses, suggesting that they play key roles in these processes. The systematic analysis of MaBAHD genes offered basic insight for further gene functional assays and potential applications in genetically improving banana cultivars.


Assuntos
Musa/genética , Desenvolvimento Vegetal/genética , Proteínas de Plantas/genética , Estresse Fisiológico/genética , Frutas/genética , Frutas/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Genoma de Planta/genética , Família Multigênica/genética , Musa/crescimento & desenvolvimento , Filogenia , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento
9.
Int J Mol Sci ; 21(24)2020 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-33317166

RESUMO

Harvested banana fruit ripened under warm temperatures above 24 °C remain green peel, leading to severe economic loss. E3 ubiquitin-ligases, as the major components in the ubiquitination pathway, have been implicated to play important roles in temperature-stress responses. However, the molecular mechanism underlying high temperature-triggered stay-green ripening bananas in association with E3 ubiquitin-ligases, remains largely unknown. In this study, a RING-type E3 ubiquitin ligase termed MaLUL2, was isolated and characterized from banana fruit. The MaLUL2 gene contains 1095 nucleotides and encodes a protein with 365 amino acids. The MaLUL2 protein contains a domain associated with RING2 (DAR2) and a RING domain, which are the typical characteristics of RING-type E3 ligases. MaLUL2 expression was up-regulated during high temperature-induced green ripening. Subcellular localization showed that MaLUL2 localized in the nucleus, cytoplasm, and plasma membrane. MaLUL2 displayed E3 ubiquitin ligase activity in vitro. More importantly, transient overexpression of MaLUL2 in banana fruit peel increased the level of ubiquitination in vivo and led to a stay-green phenotype, accompanying with decreased expression of chlorophyll catabolic genes. Collectively, these findings suggest that MaLUL2 might act as a negative regulator of chlorophyll degradation and provide novel insights into the regulatory mechanism of high temperature-induced green ripening bananas.


Assuntos
Frutas/genética , Musa/genética , Proteínas de Plantas/genética , Ubiquitina-Proteína Ligases/genética , Frutas/crescimento & desenvolvimento , Frutas/metabolismo , Resposta ao Choque Térmico , Musa/crescimento & desenvolvimento , Musa/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Domínios Proteicos , Transporte Proteico , Ubiquitina-Proteína Ligases/química , Ubiquitina-Proteína Ligases/metabolismo
10.
Sci Rep ; 10(1): 20840, 2020 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-33257717

RESUMO

MADS-box genes are critical regulators of growth and development in flowering plants. Sequencing of the Musa balbisiana (B) genome has provided a platform for the systematic analysis of the MADS-box gene family in the important banana ancestor Musa balbisiana. Seventy-seven MADS-box genes, including 18 type I and 59 type II, were strictly identified from the banana (Pisang Klutuk Wulung, PKW, 2n = 2x = 22) B genome. These genes have been preferentially placed on the banana B genome. Evolutionary analysis suggested that M. balbisiana MCM1-AGAMOUS-DEFICIENS-SRF (MbMADS) might be organized into the MIKCc, MIKC*, Mα, Mß, and Mγ groups according to the phylogeny. MIKCc was then further categorized into 10 subfamilies according to conserved motif and gene structure analyses. The well-defined MADS-box genes highlight gene birth and death in banana. MbMADSes originated from the same ancestor as MaMADSes. Transcriptome analysis in cultivated banana (ABB) revealed that MbMADSes were conserved and differentially expressed in several organs, in various fruit developing and ripening stages, and in stress treatments, indicating the participation of these genes in fruit development, ripening, and stress responses. Of note, SEP/AGL2 and AG, as well as other several type II MADS-box genes, including the STMADS11 and TM3/SOC1 subfamilies, indicated elevated expression throughout banana fruit development, ripening, and stress treatments, indicating their new parts in controlling fruit development and ripening. According to the co-expression network analysis, MbMADS75 interacted with bZIP and seven other transcription factors to perform its function. This systematic analysis reveals fruit development, ripening, and stress candidate MbMADSes genes for additional functional studies in plants, improving our understanding of the transcriptional regulation of MbMADSes genes and providing a base for genetic modification of MADS-mediated fruit development, ripening, and stress.


Assuntos
Proteínas de Domínio MADS/genética , Fatores de Transcrição MEF2/genética , Musa/genética , Sequência Conservada/genética , Evolução Molecular , Frutas/genética , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica de Plantas/genética , Genoma de Planta/genética , Família Multigênica/genética , Musa/crescimento & desenvolvimento , Filogenia , Proteínas de Plantas/genética , Fatores de Transcrição/genética
11.
Int J Mol Sci ; 21(21)2020 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-33114462

RESUMO

Edible banana cultivars are diploid, triploid, or tetraploid hybrids, which originated by natural cross hybridization between subspecies of diploid Musa acuminata, or between M. acuminata and diploid Musa balbisiana. The participation of two other wild diploid species Musa schizocarpa and Musa textilis was also indicated by molecular studies. The fusion of gametes with structurally different chromosome sets may give rise to progenies with structural chromosome heterozygosity and reduced fertility due to aberrant chromosome pairing and unbalanced chromosome segregation. Only a few translocations have been classified on the genomic level so far, and a comprehensive molecular cytogenetic characterization of cultivars and species of the family Musaceae is still lacking. Fluorescence in situ hybridization (FISH) with chromosome-arm-specific oligo painting probes was used for comparative karyotype analysis in a set of wild Musa species and edible banana clones. The results revealed large differences in chromosome structure, discriminating individual accessions. These results permitted the identification of putative progenitors of cultivated clones and clarified the genomic constitution and evolution of aneuploid banana clones, which seem to be common among the polyploid banana accessions. New insights into the chromosome organization and structural chromosome changes will be a valuable asset in breeding programs, particularly in the selection of appropriate parents for cross hybridization.


Assuntos
Coloração Cromossômica/métodos , Cromossomos de Plantas/genética , Musa/crescimento & desenvolvimento , Produtos Agrícolas/genética , Produtos Agrícolas/crescimento & desenvolvimento , Diploide , Evolução Molecular , Cariótipo , Musa/genética , Melhoramento Vegetal , Tetraploidia , Translocação Genética , Triploidia
12.
BMC Plant Biol ; 20(1): 489, 2020 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-33109087

RESUMO

BACKGROUND: Heterobeltiosis is the phenomenon when the hybrid's performance is superior to its best performing parent. Banana (Musa spp. AAA) breeding is a tedious, time-consuming process, taking up to two decades to develop a consumer acceptable hybrid. Exploiting heterobeltiosis in banana breeding will help to select breeding material with high complementarity, thus increasing banana breeding efficiency. The aim of this study was therefore to determine and document the level of heterobeltiosis of bunch weight and plant stature in the East African highland bananas, in order to identify potential parents that can be used to produce offspring with desired bunch weight and stature after a few crosses. RESULTS: This research found significant progressive heterobeltiosis in cross-bred 'Matooke' (highland cooking) banana hybrids, also known as NARITAs, when grown together across years with their parents and grandparents in Uganda. Most (all except 4) NARITAs exhibited positive heterobeltiosis for bunch weight, whereas slightly more than half of them had negative heterobeltiosis for stature. The secondary triploid NARITA 17 had the highest heterobeltiosis for bunch weight: 249% versus its 'Matooke' grandparent and 136% against its primary tetraploid parent. Broad sense heritability (across three cropping cycles) for yield potential and bunch weight were high (0.84 and 0.76 respectively), while that of plant stature was very low (0.0035). There was a positive significant correlation (P < 0.05) between grandparent heterobeltiosis for bunch weight and genetic distance between parents (r = 0.39, P = 0.036), bunch weight (r = 0.7, P < 0.001), plant stature (r = 0.38, P = 0.033) and yield potential (r = 0.59, P < 0.001). Grandparent heterobeltiosis for plant stature was significantly, but negatively, correlated to the genetic distance between parents (r = - 0.6, P < 0.001). CONCLUSIONS: Such significant heterobeltiosis exhibited for bunch weight is to our knowledge the largest among main food crops. Since bananas are vegetatively propagated, the effect of heterobeltiosis is easily fixed in the hybrids and will not be lost over time after the release and further commercialization of these hybrids.


Assuntos
Hibridização Genética , Musa/crescimento & desenvolvimento , Melhoramento Vegetal/métodos , Produção Agrícola , Frutas/genética , Frutas/crescimento & desenvolvimento , Musa/genética , Característica Quantitativa Herdável
13.
PLoS One ; 15(10): e0239058, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33001980

RESUMO

Magnesium (Mg) plays an irreplaceable role in plant growth and development. Mg transporters, especially CorA/MGT/MRS2 family proteins, played a vital role in regulating Mg content in plant cells. Although extensive work has been conducted in model crops, such as Arabidopsis, rice, and maize, the relevant information is scarce in tropical crops. In this study, 10 MaMRS2 genes in banana (Musa acuminata) were isolated from its genome and classified into five distinct clades. The putative physiochemical properties, chromosome location, gene structure, cis-acting elements, and duplication relationships in between these members were analyzed. Complementary experiments revealed that three MaMRS2 gene members (MaMRS2-1, MaMRS2-4, MaMRS2-7), from three distinct phylogenetic branches, were capable of restoring the function of Mg transport in Salmonella typhimurium mutants. Semi-quantitative RT-PCR showed that MaMRS2 genes were differentially expressed in banana cultivar 'Baxijiao' (Musa spp. AAA Cavendish) seedlings. The result was confirmed by real-time PCR analysis, in addition to tissue specific expression, expression differences among MaMRS2 members were also observed under Mg deficiency conditions. These results showed that Mg transporters may play a versatile role in banana growth and development, and our work will shed light on the functional analysis of Mg transporters in banana.


Assuntos
Proteínas de Transporte de Cátions/metabolismo , Magnésio/metabolismo , Musa/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Transporte de Cátions/genética , Mapeamento Cromossômico , Galactolipídeos/genética , Duplicação Gênica , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Genes de Plantas , Teste de Complementação Genética , Família Multigênica , Musa/genética , Musa/crescimento & desenvolvimento , Oryza/genética , Filogenia , Proteínas de Plantas/genética , Regiões Promotoras Genéticas , Leveduras/genética , Zea mays/genética
14.
J Agric Food Chem ; 68(40): 11309-11316, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-32907317

RESUMO

Banana (Musa cavendish) is one of the most popular fruits globally and is an important foodstuff in many regions, attributed to its high nutritional value. Contrast to its high consumption volume, relatively little research has been conducted on banana lipidome. In this study, two classic lipid extraction methods, Folch and Bligh-Dyer, were compared for studying the banana lipidome in both the peel and pulp by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Lipidomic profiles were also investigated to understand the changes of lipid molecules during three ripening stages (unripe, ripe, and overripe), and differences in lipids from different origins were also compared. This study suggested that although both Folch and Bligh-Dyer methods allow lipidome investigation, the latter demonstrated advantage in rendering higher extraction efficiency for the majority of lipid molecules in banana samples, particularly in the pulp. In peel, there were differences in the trends of each lipid classes at various stages of maturity, while the majority of lipid classes in pulp reached the highest levels with reduced desaturation at ripe stage, consistent with previous studies. Moreover, the lipidomic profiles of bananas in different habitats differed significantly according to partial least-squares discriminant analysis. This study for the first time provided comprehensive atlas of lipidomic changes of Musa cavendish during maturity and in different origins. These findings will facilitate better understanding of biochemical changes in banana and offer new tools for food chemical analyses in the understanding of mechanisms underlying lipid metabolism.


Assuntos
Fracionamento Químico/métodos , Frutas/crescimento & desenvolvimento , Lipídeos/química , Musa/química , Cromatografia Líquida , Frutas/química , Frutas/metabolismo , Metabolismo dos Lipídeos , Lipidômica , Lipídeos/isolamento & purificação , Musa/crescimento & desenvolvimento , Musa/metabolismo , Valor Nutritivo , Espectrometria de Massas em Tandem
15.
Food Funct ; 11(9): 8286-8296, 2020 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-32909591

RESUMO

Banana is an essential food resource in many tropical and subtropical countries. Metabolites in banana greatly influence its nutritional value and flavor. However, metabolic changes that occur in different developmental stages have not been comprehensively evaluated. In this study, widely targeted metabolomics based on multiple reaction monitoring was used in investigating dynamic changes in metabolites at three stages of fruit development. A total of 655 metabolites were identified in all the stages. A hierarchical cluster analysis of metabolites showed six clear expression patterns at the three developmental stages, and 69 up-regulated differential metabolites were identified in mature fruits compared with young and mature green fruits. A metabolic pathway analysis of differential metabolites showed significant enrichment of the flavonoid biosynthesis pathway and the phenylalanine, tyrosine, and tryptophan biosynthesis pathways. These results may serve as a reference for the isolation and identification of functional compounds from banana and for their sufficient utilization in the future.


Assuntos
Frutas/química , Frutas/crescimento & desenvolvimento , Musa/metabolismo , Frutas/metabolismo , Redes e Vias Metabólicas , Metabolômica , Musa/química , Musa/crescimento & desenvolvimento , Valor Nutritivo
16.
BMC Plant Biol ; 20(1): 402, 2020 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-32867686

RESUMO

BACKGROUND: Pollen formation and development is important for crop fertility and is a key factor for hybrid development. Previous reports have indicated that Arabidopsis thaliana TAPETUM DETERMINANT1 (AtTPD1) and its rice (Oryza sativa) homolog, OsTPD1-like (OsTDL1A), are required for cell specialization and greatly affect pollen formation and development. Little is known about the role of the TPD1 homolog in banana pollen development. RESULTS: Here, we report the identification and characterization of TPD1 homologs in diploid banana (Musa itinerans) and examine their role in pollen development by overexpressing the closest homolog, MaTPD1A. MaTPD1A exhibits high expression in stamen and localizes in the plasma membrane. MaTPD1A-overexpressing plants produce no pollen grains and smaller and seedless fruit compared to wild-type plants. Transcriptome analysis showed that in plant hormone, starch and sucrose metabolism, and linolenic acid metabolism-related pathways were affected by overexpression of MaTPD1A, and the expression of several key regulators, such as PTC1 and MYB80, which are known to affect anther development, is affected in MaTPD1A-overexpressing lines. CONCLUSIONS: Our results indicate that MaTPD1A plays an important role in pollen formation and fruit development in diploid banana, possibly by affecting the expression of some key regulators of pollen development.


Assuntos
Frutas/crescimento & desenvolvimento , Regulação da Expressão Gênica de Plantas , Musa/genética , Proteínas de Plantas/genética , Pólen/crescimento & desenvolvimento , Frutas/genética , Genes de Plantas , Musa/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Pólen/genética
17.
BMC Plant Biol ; 20(1): 432, 2020 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-32943012

RESUMO

Panama disease (Fusarium wilt disease) caused by Fusarium oxysporum f. sp. cubense race 4 (FOC) severely threatens banana (Musa spp.) production worldwide. Intercropping of banana with Allium plants has shown a potential to reduce Panama disease. In this study, six cultivars of Chinese chive (Allium tuberosum Rottler) were selected to compare their differences in antifungal activity and active compounds. Three cultivars Duokang Fujiu 11, Fujiuhuang 2, and Duokang Sijiqing with higher levels of antifungal compounds were further used for intercropping with banana in the pots and field to compare their effects on growth and disease incidence of banana.The six cultivars showed significant differences in antifungal activity against FOC mycelia growth in both leaf volatiles and aqueous leachates. The aqueous leachates displayed stronger antifungal activity than the volatiles. FJH cultivar showed the best inhibitory effect among all six cultivars. Contents of three main antifungal compounds dipropyl trisulfide (DPT), dimethyl trisulfide (DMT), and 2-methyl-2-pentenal (MP) in volatiles and aqueous leachates varied considerably among cultivars. Pot and field experiments showed that intercropping with three selected Chinese chive cultivars significantly improved banana vegetative growth, increased photosynthetic characteristics and yield but decreased disease incidence of Panama disease.Our results indicate that intercropping with Chinese chive shows potential to reduce banana Panama disease and selection of appropriate cultivars is vital for effective disease control.


Assuntos
Cebolinha-Francesa/microbiologia , Produção Agrícola/métodos , Musa/microbiologia , Doenças das Plantas/prevenção & controle , Cebolinha-Francesa/crescimento & desenvolvimento , Resistência à Doença , Fusarium , Musa/crescimento & desenvolvimento , Folhas de Planta/metabolismo
18.
Plant Physiol ; 184(2): 1153-1171, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32694134

RESUMO

Ethylene plays a critical regulatory role in climacteric fruit ripening, and its biosynthesis is fine-tuned at the transcriptional and posttranslational levels. Nevertheless, the mechanistic link between transcriptional and posttranslational regulation of ethylene biosynthesis during fruit ripening is largely unknown. This study uncovers a coordinated transcriptional and posttranslational mechanism of controlling ethylene biosynthesis during banana (Musa acuminata) fruit ripening. NAC (NAM, ATAF, and CUC) proteins MaNAC1 and MaNAC2 repress the expression of MaERF11, a protein previously known to negatively regulate ethylene biosynthesis genes MaACS1 and MaACO1 A RING E3 ligase MaXB3 interacts with MaNAC2 to promote its ubiquitination and degradation, leading to the inhibition of MaNAC2-mediated transcriptional repression. In addition, MaXB3 also targets MaACS1 and MaACO1 for proteasome degradation. Further evidence supporting the role of MaXB3 is provided by its transient and ectopic overexpression in banana fruit and tomato (Solanum lycopersicum), respectively, which delays fruit ripening via repressing ethylene biosynthesis and thus ethylene response. Strikingly, MaNAC1 and MaNAC2 directly repress MaXB3 expression, suggesting a feedback regulatory mechanism that maintains a balance of MaNAC2, MaACS1, and MaACO1 levels. Collectively, our findings establish a multilayered regulatory cascade involving MaXB3, MaNACs, MaERF11, and MaACS1/MaACO1 that controls ethylene biosynthesis during climacteric ripening.


Assuntos
Etilenos/biossíntese , Frutas/crescimento & desenvolvimento , Frutas/genética , Frutas/metabolismo , Musa/crescimento & desenvolvimento , Musa/genética , Musa/metabolismo , China , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Genes de Plantas
19.
Database (Oxford) ; 20202020 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-32283556

RESUMO

Improvement of edible bananas (a triploid and sterile crop) through conventional breeding is a challenging task owing to its recalcitrant nature for seed set, prolonged crop duration. In addition, the need of huge man power at different stages of progeny development and evaluation often leads to mislabeling, poor data management and loss of vital data. All this can be overcome by the application of advanced information technology source. This ensured secure and efficient data management such as storage, retrieval and data analysis and further could assist in tracking the breeding status in real time. Thus, a user-friendly web-based banana breeding tracker (BBT) has been developed using MySQL database with Hypertext Preprocessor (PHP). This BBT works on all operating systems with access to multiple users from anywhere at any time. Quick responsive (QR) code labels can be generated by the tracker, which can be decoded using QR scanner. Also for each and every updated progress in breeding stages, a new QR code can be generated, which in turn reduce labeling errors. Moreover, the tracker has additional tools to search, sort and filter the data from the data sets for efficient retrieval and analysis. This tracker is being upgraded with phenotypic and genotypic data that will be made available in the public domain for hastening the banana improvement program.


Assuntos
Bases de Dados Factuais , Musa/crescimento & desenvolvimento , Musa/genética , Melhoramento Vegetal/métodos , Humanos , Armazenamento e Recuperação da Informação/métodos , Internet , Ploidias , Interface Usuário-Computador
20.
Sci Rep ; 10(1): 6701, 2020 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-32317686

RESUMO

Saba banana, a popular fruit crop grown in Southeast Asia, is an economical source of a variety of beneficial agents. This study examined the variations in total phenolic, flavonoid, and antioxidant activities of five maturity stages of Saba banana, and their changes during simulated in vitro gastrointestinal digestion as affected by varying structural compositions. Antioxidant activities were evaluated using ferric reducing antioxidant power (FRAP), metal ion chelating (MIC) activity, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Results of DPPH and ABTS were compared in terms of TEAC (Trolox Equivalent Antioxidant Capacity) and VCEAC (Vitamin C Equivalent Antioxidant Capacity) values. Bio-properties were found to be highest in mature green stage with values slightly decreased as ripening proceeded. Simulated digestion showed a continuous increase in total phenolic with comparatively faster release in structure-less state (slurry) than samples with intact structure (cut). The trend of antioxidant activities was increased in the gastric phase and then decreased at the onset of intestinal phase, except for MIC which showed a reverse effect. Our study indicated that the bio-properties of Saba banana were affected by maturity and modifications in its physical structure and composition could influence the release behaviors of food components during simulated digestion.


Assuntos
Digestão/fisiologia , Trato Gastrointestinal/fisiologia , Musa/química , Musa/crescimento & desenvolvimento , Antioxidantes/metabolismo , Ácido Ascórbico/metabolismo , Quelantes/análise , Ferro/metabolismo , Oxirredução , Capacidade de Absorbância de Radicais de Oxigênio , Fenóis/análise
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