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1.
Medicine (Baltimore) ; 99(2): e18499, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31914019

RESUMO

BACKGROUND: Previous studies have reported the association between Mycoplasma fermentans (M. fermentans) and the risk of human immunodeficiency virus 1 (HIV-1) infection, but the results were inconsistent. The present study aims to systematically review reported studies on M. fermentans and its association with HIV-1 infection, as well as to summarize the findings using a meta-analysis. METHODS: Studies meeting the inclusion criteria in the PubMed, Embase, China National Knowledge Infrastructure, WanFang Data, and Chongqing VIP databases up to March 2019 were identified. Cochran Q and I statistics were used to assess heterogeneity. Additionally, pooled odds ratio (OR) with 95% confidence intervals (CI) were calculated and displayed by Forest plots. Also, the funnel plot, Begg test, and Egger test were used to evaluate potential publication bias. In addition, the source of heterogeneity was investigated by subgroup and sensitivity analyses. RESULTS: A total of 11 studies comprising 1028 HIV-1-positive patients and 1298 controls were ultimately included in this meta-analysis. Our results indicated that M. fermentans could increase the risk of HIV-1 infection among humans (OR = 3.66, 95%CI 1.26-10.64). Subgroup analysis showed that the risk of HIV-1 infection associated with M. fermentans was, based on the geographical distribution, 1.19 (95%CI 0.33-4.33) in Europe, 2.83 (95%CI 0.94-8.52) in United States, 11.92 (95%CI 3.93-36.15) in Asia; based on the source of the sample, 2.97 (95%CI 0.89-9.95) in blood samples, 4.36 (95%CI 1.63-11.68) in urine samples; based on the detection method, 2.80 (95%CI 0.72-10.96) with the polymerase chain reaction method, 5.54 (95%CI 1.21-25.28) with other detection methods; based on the source of controls, 1.91 (95%CI 0.53-6.89) in sexually transmitted diseases individuals, and 8.25 (95%CI 2.16-31.60) in health individuals. CONCLUSION: Our study revealed evidence of the association between M. fermentans and HIV-1 infection. Considering the heterogeneity, further studies are warranted to understand the relationship between M. fermentans and HIV-1 infection.


Assuntos
Infecções por HIV/etiologia , Soropositividade para HIV/diagnóstico , Infecções por Mycoplasma/complicações , Mycoplasma fermentans/metabolismo , Ásia/epidemiologia , Europa (Continente)/epidemiologia , Feminino , Infecções por HIV/diagnóstico , Soropositividade para HIV/complicações , Soropositividade para HIV/virologia , HIV-1/genética , HIV-1/isolamento & purificação , Humanos , Masculino , Infecções por Mycoplasma/microbiologia , Mycoplasma fermentans/isolamento & purificação , Fatores de Risco , Estados Unidos/epidemiologia
2.
Epidemiol Infect ; 143(15): 3327-34, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25792346

RESUMO

Mycoplasma infections are most frequently associated with disease in the urogenital or respiratory tracts and, in most cases, mycoplasmas infect the host persistently. In HIV-infected individuals the prevalence and role of genital mycoplasmas has not been well studied. To investigate the six species of Mycoplasma and the risk factors for infection in Jiangsu province, first-void urine and venous blood samples were collected and epidemiological questionnaires were administered after informed consent. A total of 1541 HIV/AIDS patients were recruited in this study. The overall infection rates of six Mycoplasma species were: Ureaplasma urealyticum (26·7%), Mycoplasma hominis (25·3%), M. fermentans (5·1%), M. genitalium (20·1%), M. penetrans (1·6%) and M. pirum (15·4%). The Mycoplasma infection rate in the unmarried group was lower than that of the married, divorced and widowed groups [adjusted odds ratio (aOR) 1·432, 95% confidence interval (CI) 1·077-1·904, P < 0·05]. The patients who refused highly active antiretroviral therapy (HAART) had a much higher risk of Mucoplasma infection (aOR 1·357, 95% CI 1·097-1·679, P < 0·05). Otherwise, a high CD4+ T cell count was a protective factor against Mycoplasma infection (aOR 0·576, 95% CI 0·460-0·719, P < 0·05). Further research will be required to confirm a causal relationship and to identify risk factors for Mycoplasma infection in HIV/AIDS populations.


Assuntos
Terapia Antirretroviral de Alta Atividade/estatística & dados numéricos , Infecções por HIV/epidemiologia , Estado Civil/estatística & dados numéricos , Infecções por Mycoplasma/epidemiologia , Infecções por Ureaplasma/epidemiologia , Adolescente , Adulto , Idoso , China/epidemiologia , Coinfecção/epidemiologia , Estudos Transversais , Infecções por HIV/tratamento farmacológico , HIV-1 , Humanos , Masculino , Pessoa de Meia-Idade , Mycoplasma/genética , Mycoplasma/isolamento & purificação , Infecções por Mycoplasma/microbiologia , Mycoplasma fermentans/genética , Mycoplasma fermentans/isolamento & purificação , Mycoplasma genitalium/genética , Mycoplasma genitalium/isolamento & purificação , Mycoplasma hominis/genética , Mycoplasma hominis/isolamento & purificação , Mycoplasma penetrans/genética , Mycoplasma penetrans/isolamento & purificação , Reação em Cadeia da Polimerase , Prevalência , Fatores de Risco , Ureaplasma urealyticum/genética , Ureaplasma urealyticum/isolamento & purificação , Adulto Jovem
3.
BMC Res Notes ; 6: 9, 2013 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-23298636

RESUMO

BACKGROUND: Mycoplasma fermentans has been associated with respiratory, genitourinary tract infections and rheumatoid diseases but its role as pathogen is controversial. The purpose of this study was to probe that Mycoplasma fermentans is able to produce respiratory tract infection and migrate to several organs on an experimental infection model in hamsters. One hundred and twenty six hamsters were divided in six groups (A-F) of 21 hamsters each. Animals of groups A, B, C were intratracheally injected with one of the mycoplasma strains: Mycoplasma fermentans P 140 (wild strain), Mycoplasma fermentans PG 18 (type strain) or Mycoplasma pneumoniae Eaton strain. Groups D, E, F were the negative, media, and sham controls. Fragments of trachea, lungs, kidney, heart, brain and spleen were cultured and used for the histopathological study. U frequency test was used to compare recovery of mycoplasmas from organs. RESULTS: Mycoplasmas were detected by culture and PCR. The three mycoplasma strains induced an interstitial pneumonia; they also migrated to several organs and persisted there for at least 50 days. Mycoplasma fermentans P 140 induced a more severe damage in lungs than Mycoplasma fermentans PG 18. Mycoplasma pneumoniae produced severe damage in lungs and renal damage. CONCLUSIONS: Mycoplasma fermentans induced a respiratory tract infection and persisted in different organs for several weeks in hamsters. This finding may help to explain the ability of Mycoplasma fermentans to induce pneumonia and chronic infectious diseases in humans.


Assuntos
Modelos Animais de Doenças , Infecções por Mycoplasma/microbiologia , Mycoplasma fermentans/isolamento & purificação , Infecções Respiratórias/microbiologia , Animais , Sequência de Bases , Cricetinae , Primers do DNA , Infecções por Mycoplasma/fisiopatologia , Reação em Cadeia da Polimerase , Infecções Respiratórias/fisiopatologia
4.
Nucleic Acid Ther ; 22(1): 58-68, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22229275

RESUMO

Artificial RNA reagents such as small interfering RNAs (siRNAs) and aptamers often must be chemically modified for optimal effectiveness in environments that include ribonucleases. Mycoplasmas are common bacterial contaminants of mammalian cell cultures that are known to produce ribonucleases. Here we describe the rapid degradation of nuclease-stabilized RNA oligonucleotides in a human embryonic kidney 293 (HEK) cell culture contaminated with Mycoplasma fermentans, a common species of mycoplasma. RNA with 2'-fluoro- or 2'-O-methyl- modified pyrimidines was readily degraded in conditioned media from this culture, but was stable in conditioned media from uncontaminated HEK cells. RNA completely modified with 2'-O-methyls was not degraded in the mycoplasma-contaminated media. RNA zymogram analysis of conditioned culture media and material centrifuged from the media revealed several distinct protein bands (ranging from 30 to 68 kDa) capable of degrading RNA with 2'-fluoro- or 2'-O-methyl-modified pyrimidines. Finally, the mycoplasma-associated nuclease was detected in material centrifuged from the contaminated culture supernatants in as little as 15 minutes with an RNA oligo-containing 2'-O-methyl-modified pyrimidines and labeled with a 5'-fluorescein amidite (FAM) and 3'-quencher. These results suggest that mycoplasma contamination may be a critical confounding variable for cell culture experiments involving RNA-based reagents, with particular relevance for applications involving naked RNA (e.g., aptamer-siRNA chimeras).


Assuntos
Meios de Cultura , Mycoplasma fermentans/isolamento & purificação , Linhagem Celular , Humanos , Reação em Cadeia da Polimerase , Proteólise
5.
BMC Musculoskelet Disord ; 10: 97, 2009 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-19650889

RESUMO

BACKGROUND: Increasing evidence incriminates bacteria, especially Mycoplasma fermentans, as possible arthritogenic agents in humans. The purpose of this study was to investigate M. fermentans in the bloodstream of patients with rheumatoid arthritis. METHODS: Two hundred and nineteen blood samples from patients with rheumatoid arthritis, systemic lupus erythematosus, antiphospholipid syndrome, and healthy individuals were screened by bacterial culture and direct PCR in order to detect mycoplasmas; IgM and IgG against M. fermentans PG18 were also detected by ELISA and Immunoblotting assays in patients with rheumatoid arthritis and healthy individuals. RESULTS: Blood samples from patients with antiphospholipid syndrome and healthy individuals were negative for mycoplasma by culture or direct PCR. In blood samples from patients with systemic lupus erythematosus were detected by direct PCR M. fermentans in 2/50 (2%), M. hominis in 2/50 (2%) and U. urealyticum in 1/50 (0.5%). In patients with RA M. fermentans was detected by culture in 13/87 blood samples and in 13/87 by direct PCR, however, there was only concordance between culture and direct PCR in six samples, so M. fermentans was detected in 20/87(23%) of the blood samples from patients with RA by either culture or PCR. Antibody-specific ELISA assay to M. fermentans PG18 was done, IgM was detected in sera from 40/87 patients with RA and in sera of 7/67 control individuals, IgG was detected in sera from 48/87 RA patients and in sera from 7/67 healthy individuals. Antibody-specific immunoblotting to M. fermentans PG18 showed IgM in sera from 35/87 patients with RA and in sera from 4/67 healthy individuals, IgG was detected in sera from 34/87 patients and in sera from 5/67 healthy individuals. CONCLUSION: Our findings show that only M. fermentans produce bacteremia in a high percentage of patients with RA. This finding is similar to those reported in the literature. IgM and IgG against M. fermentans PG18 were more frequent in patients with RA than healthy individuals.


Assuntos
Grupo com Ancestrais Nativos do Continente Americano , Anticorpos Antibacterianos/sangue , Artrite Reumatoide/microbiologia , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Mycoplasma fermentans/imunologia , Adulto , Idoso , Síndrome Antifosfolipídica/etnologia , Síndrome Antifosfolipídica/microbiologia , Artrite Reumatoide/tratamento farmacológico , Artrite Reumatoide/etnologia , Estudos de Casos e Controles , DNA Bacteriano/sangue , Ensaio de Imunoadsorção Enzimática , Humanos , Lúpus Eritematoso Sistêmico/etnologia , Lúpus Eritematoso Sistêmico/microbiologia , México , Pessoa de Meia-Idade , Mycoplasma fermentans/genética , Mycoplasma fermentans/isolamento & purificação , Mycoplasma hominis/imunologia , Reação em Cadeia da Polimerase , Ureaplasma urealyticum/imunologia
6.
Antimicrob Agents Chemother ; 53(5): 2139-41, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19258276

RESUMO

MICs were determined for an investigational ketolide, CEM-101, and azithromycin, telithromycin, doxycycline, levofloxacin, clindamycin, and linezolid against 36 Mycoplasma pneumoniae, 5 Mycoplasma genitalium, 13 Mycoplasma hominis, 15 Mycoplasma fermentans, and 20 Ureaplasma isolates. All isolates, including two macrolide-resistant M. pneumoniae isolates, were inhibited by CEM-101 at < or = 0.5 microg/ml, making CEM-101 the most potent compound tested.


Assuntos
Antibacterianos/farmacologia , Cetolídeos/farmacologia , Mycoplasma/efeitos dos fármacos , Ureaplasma/efeitos dos fármacos , Farmacorresistência Bacteriana , Humanos , Testes de Sensibilidade Microbiana , Mycoplasma/classificação , Mycoplasma/isolamento & purificação , Infecções por Mycoplasma/microbiologia , Mycoplasma fermentans/efeitos dos fármacos , Mycoplasma fermentans/isolamento & purificação , Mycoplasma genitalium/efeitos dos fármacos , Mycoplasma genitalium/isolamento & purificação , Mycoplasma pneumoniae/efeitos dos fármacos , Ureaplasma/classificação , Ureaplasma/isolamento & purificação , Infecções por Ureaplasma/microbiologia
7.
Antimicrob Agents Chemother ; 52(10): 3776-8, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18663020

RESUMO

The in vitro susceptibilities of 151 unique clinical isolates of Mycoplasma pneumoniae, Mycoplasma hominis, Mycoplasma fermentans, Mycoplasma genitalium, and Ureaplasma species to DC-159a, an investigational fluoroquinolone, in comparison with those to other agents were determined. Macrolides were the most active agents against M. pneumoniae and M. genitalium, whereas clindamycin was most active against M. hominis. DC-159a MICs were or=99.9% of the inoculum at 24 h for 2 isolates. The excellent in vitro activity of DC-159a demonstrates its potential for use in the treatment of infections due to mycoplasmas and ureaplasmas.


Assuntos
Aminopiridinas/farmacologia , Antibacterianos/farmacologia , Fluoroquinolonas/farmacologia , Mycoplasma/efeitos dos fármacos , Ureaplasma/efeitos dos fármacos , Humanos , Técnicas In Vitro , Testes de Sensibilidade Microbiana , Mycoplasma/isolamento & purificação , Infecções por Mycoplasma/tratamento farmacológico , Infecções por Mycoplasma/microbiologia , Mycoplasma fermentans/efeitos dos fármacos , Mycoplasma fermentans/isolamento & purificação , Mycoplasma genitalium/efeitos dos fármacos , Mycoplasma genitalium/isolamento & purificação , Mycoplasma hominis/efeitos dos fármacos , Mycoplasma hominis/isolamento & purificação , Mycoplasma pneumoniae/efeitos dos fármacos , Mycoplasma pneumoniae/isolamento & purificação , Ureaplasma/isolamento & purificação , Infecções por Ureaplasma/tratamento farmacológico , Infecções por Ureaplasma/microbiologia
8.
Biologicals ; 36(2): 117-21, 2008 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17892949

RESUMO

A panel of 30 putative Mycoplasma fermentans strains, isolated from various sources including human, ovine and cell lines, were tested by a previously described polymerase chain reaction (PCR) to confirm their identity by amplification of a conserved 206 bp region of the insertion sequence IS1550. In addition, the application of another PCR based on the major part of the IS1550 element showed one or two products of different length (1144 and 1341 bp) enabling M. fermentans strains to be divided into two types designated as Type A and Type B. A PCR, which amplifies the macrophage activating lipopeptide gene (malp), supported the identification of all the strains as M. fermentans. Thirteen other species of Mycoplasma from human sources gave negative results in these tests, with the exception of Mycoplasma orale, which was detected by both IS1550-PCRs based on the major part and the conserved 206 bp region of the IS1550 element. This study suggests that all M. fermentans isolates possess both the IS1550 element and the malp gene. In contrast to the IS1550, the malp gene is shown to be species-specific and the use of a malp PCR described here could prove to be a useful adjunct to IS1550 detection as confirmation of the presence of M. fermentans in clinical material.


Assuntos
Mycoplasma fermentans/genética , Mycoplasma fermentans/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Animais , Linhagem Celular , DNA Bacteriano/genética , DNA Ribossômico/genética , Humanos , Mycoplasma fermentans/classificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Ovinos/microbiologia
9.
Immunology ; 123(2): 187-96, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17680797

RESUMO

The aim of this work was to discover if Mycoplasma fermentans, which is known to infect B cells, could be the cause of the raised ecto-5'-nucleotidase observed in the synovial fluid of rheumatoid arthritis patients. The ecto-5'-nucleotidase activity in the patients' serum has been shown to correlate with the erythrocyte sedimentation rate and DNA from the mycoplasma has been found in the synovial fluid. B lymphoblastoid cell lines were exposed to 16 strains of Mycoplasma fermentans and their ecto-5'-nucleotidase, CD73, was measured both biochemically and by mouse antibodies to human ecto 5'-nucleotidase using the fluorescence activated cell sorter. The type strain, PG 18, did not grow with the B cells. Some of the mycoplasma strains (9/15) increased the cellular ecto-5'-nucleotidase activity from twice to 17 fold, and usually showed 5'-nucleotidase activity themselves. At least one strain, M106, induced human 5'-nucleotidase on the normally 5'-nucleotidase negative Daudi and Raji Burkitt's lymphoma cell lines, and increased sevenfold the 5'-nucleotidase on the monocyte/macrophage cell line THP-1. Growing the cells in aged medium increased the level of mycoplasma infection. This mycoplasma-induced enzyme showed a conformational change and an increase in activity with a glycosylation change involving mannose groups. The other group of strains, mostly of respiratory or cell culture origin, usually did not have any 5'-nucleotidase of their own and decreased the B-cell enzyme activity by about half. Electron microscopy and flow cytometry showed that the strain M106 was filamentous and could be found inside the B-cells. The 5'-nucleotidase-inducing strains of M. fermentans may be important in the aetiology of rheumatoid arthritis.


Assuntos
5'-Nucleotidase/metabolismo , Artrite Reumatoide/microbiologia , Linfócitos B/microbiologia , Infecções por Mycoplasma/complicações , Mycoplasma fermentans/isolamento & purificação , Artrite Reumatoide/enzimologia , Artrite Reumatoide/imunologia , Linfócitos B/imunologia , Linfócitos B/ultraestrutura , Linhagem Celular , Células Cultivadas , Humanos , Microscopia Eletrônica , Infecções por Mycoplasma/enzimologia , Infecções por Mycoplasma/imunologia , Mycoplasma fermentans/classificação , Mycoplasma fermentans/ultraestrutura , Células Tumorais Cultivadas
12.
Prog. obstet. ginecol. (Ed. impr.) ; 48(5): 239-246, mayo 2005. tab
Artigo em Espanhol | IBECS | ID: ibc-036885

RESUMO

Objetivo: Investigar la prevalencia serológica de Mycoplasmas genitalium, M. fermentans y M. penetrans en mujeres con esterilidad, y compararla con la de mujeres fértiles. Material y métodos: Estudiamos a 55 mujeres estériles por factor tuboperitoneal y a 55 mujeres clínicamente fértiles, evaluando la prevalencia serológica dentro de cada grupo y comparándolas. Resultados: La prevalencia, comparando grupo estéril y fértil fue, respectivamente: IgM: M. genitalium (27,27 y 30,91%; p = 0,152), M. fermentans (83,64 y 61,82%; p = 0,006), M. penetrans (38,18 y 49,09%; p = 0,079); IgA: M. genitalium (5,45 y 1,82%; p = 0,250), M. fermentans (0,00 y 12,73%; p = 0,006), M. penetrans (36,36 y 3,64%; p < 0,001), e IgG: M. genitalium (92,73 y 92,73%; p = 0,284), M. fermentans (65,45 y 40,00%; p = 0,004), M. penetrans (96,36 y 9,09%; p < 0,001). Conclusión: Para M. genitalium no hubo diferencia estadística entre ambos grupos. Las IgG están significativamente más elevadas en el grupo estéril que en el grupo fértil para M. fermentans y M. penetrans, lo que los relaciona con una mayor probabilidad etiológica o factores de riesgo de enfermedad inflamatoria pelviana y consecuentemente a esterilidad


Objectives: To investigate the serological prevalence of Mycoplasmas genitalium, M. fermentans and M. penetrans in infertile women compared with fertile women. Material and methods: We studied 55 women with infertility due to peritoneal-tubal factors and 55 fertile women. The serological prevalence in each group was evaluated and the results were compared. Results: The prevalence of IgM in the infertile and fertile groups was, respectively: M. genitalium (27.27% vs 30.91%; p = 0.152), M. fermentans (83.64% vs 61.82%; p = 0.006), M. penetrans (38.18% vs 49.09%; p = 0.079). IgA: M. genitalium (5.45% vs 1.82%; p = 0.250), M. fermentans (0.00% vs 12.73%; p = 0.006), M. penetrans (36.36% vs 3.64%; p < 0.001). IgG: M. genitalium (92.73% vs 92.73%; p = 0.284), M. fermentans (65.45% vs 40.00%; p = 0.004), M. penetrans (96.36% vs 9.09%; p < 0.001). Conclusions: No statistically significant differences were found in the prevalence of M. genitalium between the infertile and the fertile groups. IgG for M. fermentans and M. penetrans were significantly higher in the infertile group than in the fertile group, suggesting that these microorganisms could be the cause of, or risk factors for, pelvic inflammatory disease and female infertility


Assuntos
Feminino , Adulto , Humanos , Mycoplasma genitalium/patogenicidade , Infecções por Mycoplasma/epidemiologia , Infertilidade Feminina/microbiologia , Mycoplasma fermentans/isolamento & purificação , Mycoplasma penetrans/isolamento & purificação , Mycoplasma genitalium/isolamento & purificação , Estudos de Casos e Controles , Doença Inflamatória Pélvica/microbiologia , Estudos Transversais
13.
Beijing Da Xue Xue Bao Yi Xue Ban ; 37(2): 207-10, 2005 Apr 18.
Artigo em Chinês | MEDLINE | ID: mdl-15841158

RESUMO

OBJECTIVE: To detect the rate of Mycoplasma infection in cell lines and further determine its types. METHODS: We performed nest PCR amplification of Mycoplasma's conserved regions (16S-23S) and sequenced the spacer with different length between conserved regions. RESULTS: Within the tested 22 cell lines, 17 (77.3%) showed Mycoplasma infections, of which 5 had two or more types of Mycoplasma. M. fermentans and hyorhinis were more frequently detected within the types of infected Mycoplasma. CONCLUSION: The high rate of Mycoplasma infection in cell lines makes it necessary for researchers to pay more attention to its influence on research data when using cell lines as models. Establishment of detection and classifying techniques make it possible to further study the pathogenesis of different types of Mycoplasma.


Assuntos
Mycoplasma/classificação , Mycoplasma/isolamento & purificação , Sequência de Bases , Linhagem Celular , Humanos , Dados de Sequência Molecular , Infecções por Mycoplasma/microbiologia , Infecções por Mycoplasma/patologia , Mycoplasma fermentans/isolamento & purificação , Mycoplasma hyorhinis/isolamento & purificação , Reação em Cadeia da Polimerase , RNA Ribossômico 16S/genética , RNA Ribossômico 23S/genética
14.
Lik Sprava ; (5-6): 28-32, 2005.
Artigo em Ucraniano | MEDLINE | ID: mdl-16396287

RESUMO

The study of persistence in mononuclear leukocytes (ML) of blood and synovial fluid of 218 patients with rheumatoid arthritis (RA) Cytornegalovirus (CMV), the 1-st and 2-nd types of Herpes virus simplex (VH), Epstain-Barr virus (VEB), Mycoplasma arthritidis (Ma), Mycoplasma fermentans (Mf), Ureaplasma urealiticum (U), Chlamidia trachomatis (Ct), viruses of Hepatitis B and C was carry out by direct and indirect immunofruorescence, immunoenzymatic analysis and polymerase chain reaction. An increased frequency of contamination of blood ML with infectious agents in patients with RA was established (57,4% compared with 16,7% in control group). The following infectious agents were revieled more frequently: in ML of blood and synovial fluid the Ma (relatively 20,5% and 15,9%), Mf (15,6% and 13,2%), Ct (18,4% and 13,2%), VH (27,1% and 10,5%), VEB (12,7% and 5,3%) and CMV (11,2% and 7,9%). Types of frequency dynamics of ML contamination with these infectious agents in different time phases of RA were determined.


Assuntos
Artrite Reumatoide/sangue , Leucócitos Mononucleares/microbiologia , Líquido Sinovial/microbiologia , Anticorpos Antibacterianos/análise , Antígenos de Bactérias/análise , Antígenos Virais/análise , Chlamydia trachomatis/imunologia , Chlamydia trachomatis/isolamento & purificação , Citomegalovirus/imunologia , Citomegalovirus/isolamento & purificação , DNA Bacteriano/análise , DNA Viral/análise , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Hepacivirus/imunologia , Hepacivirus/isolamento & purificação , Vírus da Hepatite B/imunologia , Vírus da Hepatite B/isolamento & purificação , Herpes Simples/classificação , Herpes Simples/imunologia , Herpesvirus Humano 4/imunologia , Herpesvirus Humano 4/isolamento & purificação , Humanos , /virologia , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/virologia , Masculino , Mycoplasma arthritidis/imunologia , Mycoplasma arthritidis/isolamento & purificação , Mycoplasma fermentans/imunologia , Mycoplasma fermentans/isolamento & purificação , Reação em Cadeia da Polimerase , RNA Viral/análise , Estudos Retrospectivos , Líquido Sinovial/imunologia , Líquido Sinovial/virologia , Ucrânia/epidemiologia , Ureaplasma urealyticum/imunologia , Ureaplasma urealyticum/isolamento & purificação
15.
Cancer Sci ; 95(4): 311-5, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15072588

RESUMO

We aimed to determine whether mycoplasmas are present in Korean chronic gastritis, and to understand their roles in gastric cancer tumorigenesis, because mycoplasmas resemble Helicobacter pylori in terms of ammonia production and induction of inflammatory cytokines in immune and non-immune cells. The presence and identity of mycoplasmas were assessed by semi-nested PCR and sequencing, and the results were compared with pathologic data. Fifty-six samples collected from Korean chronic gastritis patients were used for this study. Twenty-three (41.1%) were positive for mycoplasmas. Eighteen sequenced samples contained a single human mycoplasma or two mycoplasmas, which were identified as Mycoplasma faucium (13/18), M. fermentans (3/18), M. orale (1/18), M. salivarium (2/18), and M. spermatophilum (1/18). Mycoplasma-infected chronic gastritis samples showed significantly more severe neutrophil infiltration than non-infected samples (P = 0.0135). Mycoplasma profiles in the oral cavity (M. salivarium is major) and stomach were different, and the presence of significant proinflammatory responses in mycoplasma-positive patients suggests that the mycoplasmas are not simply contaminants. Further studies are required to understand whether mycoplasmas play a role in gastric tumorigenesis.


Assuntos
DNA Bacteriano/isolamento & purificação , Gastrite/microbiologia , Infecções por Mycoplasma/microbiologia , Mycoplasma/isolamento & purificação , Neoplasias Gástricas/etiologia , Doença Crônica , Gastrite/metabolismo , Gastroscopia , Humanos , Coreia (Geográfico)/epidemiologia , Dados de Sequência Molecular , Boca/microbiologia , Mycoplasma/genética , Mycoplasma/patogenicidade , Infecções por Mycoplasma/epidemiologia , Infecções por Mycoplasma/genética , Mycoplasma fermentans/genética , Mycoplasma fermentans/isolamento & purificação , Mycoplasma fermentans/patogenicidade , Mycoplasma salivarium/genética , Mycoplasma salivarium/isolamento & purificação , Mycoplasma salivarium/patogenicidade , Especificidade de Órgãos , Antro Pilórico/microbiologia , Análise de Sequência de DNA , Estômago/microbiologia
17.
Rev. cuba. med ; 42(3): s.p, may.-jun. 2003. tab, graf
Artigo em Espanhol | CUMED | ID: cum-23106

RESUMO

Se realizó un estudio prospectivo en 60 pacientes VIH/SIDA con manifestaciones respiratorias, en el período comprendido entre marzo y julio de 1998; se encontró que 23 (38,3 por ciento) tenían resultados positivos para micoplasmas y que la forma clínica que predominó en estos pacientes fue la infección respiratoria superior. Se les dio de alta mejorados al 56,6 por ciento y 8 pacientes fallecieron (34,7 por ciento). El conteo de linfocitos CD4+ menor de 200 cél/mm3 se presentó en el 69,6 por ciento. El infiltrado bronconeumónico fue el hallazgo radiológico más frecuente. La coinfección con otras bacterias estuvo presente en 15 de los casos. Mycoplasma fermentans fue la especie encontrada con mayor frecuencia y los pacientes infectados con esta especie estaban severamente inmunodeprimidos, además de colonizados por otros gérmenes, entre los cuales predominó la Pseudomona aeruginosa(AU)


Assuntos
Humanos , Masculino , Feminino , Infecções por Mycoplasma/complicações , Síndrome de Imunodeficiência Adquirida/complicações , Infecções Respiratórias/complicações , Infecções Oportunistas Relacionadas com a AIDS , Mycoplasma fermentans/isolamento & purificação
19.
Rheumatology (Oxford) ; 40(12): 1355-8, 2001 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11752504

RESUMO

OBJECTIVES: To search for evidence that Mycoplasma fermentans is involved in the pathogenesis of some forms of human arthritis by testing for the presence of mycoplasmal DNA in joint material. METHODS: M. fermentans DNA was detected by the identification of a 104-base pair amplification product of the polymerase chain reaction (PCR). RESULTS: M. fermentans DNA was detected in synovial fluid samples from six (17%) of 35 patients with rheumatoid arthritis (RA) and 18 (21%) of 85 patients with seronegative arthritis. These detection rates were significantly greater than in samples from patients with osteoarthritis or crystal synovitis, none of 26 of these being positive. CONCLUSIONS: M. fermentans could be involved in the pathogenesis of some forms of inflammatory arthritis and this possibility is worthy of further study.


Assuntos
Artrite Infecciosa/epidemiologia , Artrite Reumatoide/epidemiologia , Infecções por Mycoplasma/epidemiologia , Mycoplasma fermentans/isolamento & purificação , Doença Aguda , Adulto , Idoso , Artrite Gotosa/epidemiologia , Artrite Gotosa/microbiologia , Artrite Infecciosa/microbiologia , Artrite Reumatoide/microbiologia , DNA Bacteriano/análise , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mycoplasma fermentans/genética , Osteoartrite/epidemiologia , Osteoartrite/microbiologia , Prevalência , Líquido Sinovial/microbiologia , Sinovite/epidemiologia , Sinovite/microbiologia
20.
Zhonghua Yi Xue Za Zhi ; 81(24): 1516-8, 2001 Dec 25.
Artigo em Chinês | MEDLINE | ID: mdl-16200779

RESUMO

OBJECTIVE: To study if infection of new type pathogenic mycoplasmas, Mycoplasma penetrans (Mpe), Mycoplasma fermentans (Mf), and Mycoplasma pirum (Mpi), exists in the genital tract of gynecologic patients with genital infection in China so as to provide information for clinical diagnosis and therapy. METHODS: Cervical secretions from 172 patients with infectious diaseses of gentital tract and 172 healthy controls were collected and inoculated in improved SP-4 culture medium. Biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR) assay, and DNA sequencing were employed to identify the isolated microorganisms. RESULTS: One strain of Mycoplasma penetrans (0.58%) and two strains of Mycoplasma fermentans (1.16%) were isolated from the cervical secretions of patients, but none was found in healthy controls. CONCLUSION: Mpe infection and Mf infection exist in female patients with genital infection in Nanjing.


Assuntos
Infecções por Mycoplasma/microbiologia , Mycoplasma fermentans/isolamento & purificação , Mycoplasma penetrans/isolamento & purificação , Cervicite Uterina/microbiologia , Vaginite/microbiologia , Adulto , Feminino , Humanos , Pessoa de Meia-Idade
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