Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Mais filtros

Base de dados
Tipo de estudo
Intervalo de ano de publicação
J Appl Microbiol ; 116(5): 1129-36, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24517235


AIM: To study the effects exerted by argon microwave nonthermal plasma (NTP) on cell wall-lacking Mollicutes bacteria. METHODS AND RESULTS: 10(8) CFU ml(-1) agar plated Mycoplasma hominis and Acholeplasma laidlawii were treated with the nonthermal microwave argon plasma for 30-300 s. The maximal 10- and 100-fold drop was observed for A. laidlawii and Myc. hominis, respectively. Similarly treated Escherichia coli and Staphylococcus aureus demonstrated the 10(5) and 10(3) drop, respectively. Removal of cholesterol affected resistance of A. laidlawii. 10 mmol l(-1) antioxidant butylated hydroxytoluene decreased mortality by a factor of 25-200. UV radiation alone caused 25-85% mortality in comparison with the whole NTP. Exogenously added hydrogen peroxide H2O2 did not cause mortality. NTP treatment of Myc. hominis triggered growth of microcolonies, which were several tenfold smaller than a typical colony. CONCLUSIONS: Despite the lack of cell wall, A. laidlawii and Myc. hominis were more resistant to argon microwave NTP than other tested bacteria. Mycoplasma hominis formed microcolonies upon NTP treatment. A role of UV and active species was demonstrated. SIGNIFICANCE AND IMPACT OF THE STUDY: The first study of NTP effects on Mollicutes revealed importance of a membrane composition for bacterial resistance to NTP. New specific Myc. hominis morphological forms were observed. The study confirmed importance of the concerted action of reactive oxygen species (ROS) with UV and other plasma bioactive agents for NTP bactericidal action.

Acholeplasma laidlawii/efeitos dos fármacos , Antibacterianos/farmacologia , Mycoplasma hominis/efeitos dos fármacos , Gases em Plasma/farmacologia , Argônio , Colesterol/fisiologia , Viabilidade Microbiana/efeitos dos fármacos , Micro-Ondas , Mycoplasma hominis/crescimento & desenvolvimento , Mycoplasma hominis/ultraestrutura , Oxidantes/farmacologia , Raios Ultravioleta
J Clin Microbiol ; 47(9): 3050-2, 2009 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-19641070


Mycoplasma hominis most frequently causes diseases of the genitourinary tract. Extragenital infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to trauma or surgery. We present the case of a previously well man who developed an M. hominis-associated parapharyngeal abscess following acute Epstein-Barr virus infection.

Abscesso/microbiologia , Infecções por Vírus Epstein-Barr/complicações , Infecções por Mycoplasma/diagnóstico , Mycoplasma hominis/isolamento & purificação , Faringite/microbiologia , Humanos , Masculino , Microscopia Eletrônica de Transmissão , Infecções por Mycoplasma/microbiologia , Mycoplasma hominis/ultraestrutura , Pescoço/diagnóstico por imagem , Radiografia , Tomografia , Adulto Jovem
Tsitologiia ; 51(3): 247-56, 2009.
Artigo em Russo | MEDLINE | ID: mdl-19435279


Localization of the protein FtsZ in Mycoplasma hominis cells was determined. Ultra thin sections were treated by rabbit polyclonal antibodies against FtsZ M. hominis: a conjugate of protein A with colloidal gold particles was used instead of secondary antibodies. Considerable polymorphism of cells was seen on electron microscopy pictures of M. hominis cells, which is typical for mycoplasmas. Among a wide variety of cell shapes we distinguished dumbbell-shaped dividing cells, and the cells connected with each other with the aid of thin membrane tubules (former constrictions). Dominants distribution of the label in the constriction area of dividing M. hominis cells and in the area of the thin membrane tubules was observed. We revealed the cross septum in the mycoplasma cells for the first time, as well as the gold labeling of this structure. Furthermore, in some rounded and oval cells colloidal gold particles labeled the whole plasma membrane in ring-shaped manner. Probably, the label in these cases marks a submembrane contractile ring (Z-ring). The facts mentioned above confirm that FtsZ of M. hominis plays an active role in the mycoplasma cytokinesis. In a series of cases spiral-like distribution of gold particles was observed. Probably, FtsZ protofilaments in M. hominis cells can form spiral structures similar to Z-spirals of Bacillus subtilis and Escherichia coli. Its presence in mycoplasma cells may be considered as an important argument in favour of model of Z-ring assembling through reorganization of Z-spirals. FtsZ also may participate in maintenance of mycoplasma cell shape (membrane localization).

Proteínas de Bactérias/metabolismo , Proteínas do Citoesqueleto/metabolismo , Mycoplasma hominis/metabolismo , Proteínas de Bactérias/ultraestrutura , Proteínas do Citoesqueleto/ultraestrutura , Microscopia Imunoeletrônica , Mycoplasma hominis/ultraestrutura