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1.
J Photochem Photobiol B ; 202: 111685, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31810035

RESUMO

Surface tailored GaAu loaded mesoporous silica nanoparticles are considered as an important nanomaterial for biomedical applications such as diagnosis and cancer treatment. In this study, we used GaAu loaded mesoporous silica nanoparticles (Ga-Au@mSiO2) for the photothermal treatment of two prostate cancer cell lines. We systematically examined the nanocomposite form by various spectroscopic (UV-Vis, TGA and DTA) and electroscopic techniques (TEM and SEM including the elemental mapping analysis). After careful evaluation of the nanocomposite form, we performed cancer cell growth inhibition properties of the prostate cancer cell lines (DU145 and LNCaP). Also, we performed the photothermal effects of these nanocomposites on cell proliferation and apoptosis using different biochemical staining and flow cytometry. Our in vitro investigational datas are established Ga-Au@mSiO2 effectively exhibited and also with Ga-Au@mSiO2 + NIR the photothermal conversion therapy improved prostate cancer cells abolishing the prostate cancer cells. Interestingly, Ga-Au@mSiO2 + NIR was found to surpass the activity of Ga-Au@mSiO2 in all the cancer cells tested a topnotches. Hence, our current results demonstrated that surface tailored GaAu loaded mesoporous silica nanoparticles significantly inhibited the growth of prostate cancer cell lines and shown prominent antitumor effect in vitro. Thus, our study suggests that Ga-Au@mSiO2 + NIR could be used as impending anticancer candidate for photothermal ablation of prostate cancer cells. Further examinations of the mechanism indicated that anticancer activity was accomplished by inducing apoptosis in cancer cells, which is suggesting that these Ga-Au@mSiO2 + NIR nanocomposite can be used as promising candidates for nursing care cancer therapy.


Assuntos
Gálio/química , Ouro/química , Raios Infravermelhos , Nanocompostos/química , Neoplasias da Próstata/terapia , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Pontos de Checagem do Ciclo Celular/efeitos da radiação , Linhagem Celular Tumoral , Dano ao DNA/efeitos dos fármacos , Dano ao DNA/efeitos da radiação , Humanos , Masculino , Nanocompostos/uso terapêutico , Nanocompostos/toxicidade , Cuidados de Enfermagem , Fototerapia , Porosidade , Neoplasias da Próstata/patologia , Dióxido de Silício/química
2.
J Photochem Photobiol B ; 201: 111651, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31683166

RESUMO

Designing biomimetic biomaterials influenced by the common complex structure of hard tissues is yet a test these days. The control of bio-mineralization procedure onto biomaterials should be assessed before the use in medical applications. Coming to the bone rejuvenation applications, this work assessed the in vitro antibacterial activity and interacting between osteoblast cells (MG63) on poly (hydroxypropyl methacrylate) (PHPMA) cryogel consolidated with Zn/Ce substituted hydroxyapatite (MHAp) nanocomposite (PHPMA/MHAp). Osteoblast cell multiplication, morphology, and metabolic action were assessed through various conventions. The functional group, texture, mechanical properties, and protein adsorption profiles of the fabricated nanocomposite were analyzed by the FTIR, XRD, SEM, and mechanical examinations, respectively. The bacterial activity of nanocomposites was additionally assessed against E. coli and S. aureus microorganisms, individually. Nanocomposite advanced endo-chondral ossification at the messed up parts of the bone deformity than cryogel did. These results recommend that PHPMA/MHAp nanocomposites joined the good innate properties of each polymer and bioceramic, giving a mechanically powerful, cell-responsive, and permeable stage for hard tissue applications.


Assuntos
Criogéis/química , Durapatita/química , Fraturas do Fêmur/terapia , Nanocompostos/química , Nanopartículas/química , Animais , Regeneração Óssea/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Cério/química , Modelos Animais de Doenças , Escherichia coli/efeitos dos fármacos , Fraturas do Fêmur/patologia , Nanocompostos/uso terapêutico , Nanocompostos/toxicidade , Polímeros/química , Ratos , Staphylococcus aureus/efeitos dos fármacos , Engenharia Tecidual , Zinco/química
3.
Carbohydr Polym ; 226: 115281, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31582066

RESUMO

In this work, tumor acidity and CD44 dual targeting hyaluronic acid-coated gold nanorods (AuNRs) are investigated for combined chemo- and photothermal cancer therapy. Low molecular weight hyaluronic acid (LMWHA) is conjugated with pH-sensitive groups for pH-induced aggregation and lipoic acid for coating of AuNRs. By changing pH-sensitive groups with different pKa values, pH-sensitivity of modified LMWHA can be tuned. After coating modified LMWHA onto AuNRs, biocompatibility of the AuNRs is significantly improved. These LMWHA-coated AuNRs can gradually aggregate under slightly acidic conditions, making them favorable for accumulation at acidic tumor sites. Surface LMWHA allows the nanocomposites to be selectively uptaken by CD44-expressing cancer cells, and AuNRs endows the nanocomposites with excellent photothermal ability. Loading of doxorubicin, a chemical drug, provides the LMWHA-coated AuNRs synergistic cancer cell-killing (in vitro) and tumor growth inhibiting (in vivo) ability. Taken together, these results demonstrate that this multifunctional nanosystem with pH-induced aggregation and CD44 targeting has potential for combined chemo- and photothermal cancer therapy.


Assuntos
Doxorrubicina/administração & dosagem , Sistemas de Liberação de Medicamentos/métodos , Receptores de Hialuronatos/metabolismo , Animais , Linhagem Celular Tumoral , Quimioterapia Combinada , Ouro , Humanos , Ácido Hialurônico/uso terapêutico , Camundongos , Camundongos Endogâmicos BALB C , Nanocompostos/uso terapêutico , Nanotubos , Neoplasias/terapia , Fototerapia
4.
Nanoscale ; 11(40): 18845-18853, 2019 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-31595915

RESUMO

A multifunctional nanosensor of CPs@MnO2-AgNPs was constructed for sensitive and selective sensing of GSH and cancer theranostics in this work. The CPs@MnO2 nanocomposite was synthesized by capping MnO2 onto carbon nanoparticles through an in situ redox reaction under ultrasonication. AgNPs with fluorescence were obtained through a silver-mirror-like reaction using BSA as both a template and reductant and further anchored onto the surface of CPs@MnO2 through electrostatic interaction to construct the CPs@MnO2-AgNP nanocomposite. The fluorescence of AgNPs was effectively quenched by MnO2 through an inner filter effect and a static quenching effect and further recovered by GSH owing to the unique redox reaction between GSH and MnO2. Therefore, a novel fluorescent turn-on nanosensor was established for GSH sensing in vitro and in vivo. For GSH sensing, a satisfactory linear range of 0.8-80 µM with a detection limit of 0.55 µM was obtained under optimal conditions. Moreover, by integrating the GSH-responsive fluorescence imaging capacity, the photothermal activity of carbon nanoparticles and the anticancer effect of AgNPs, the CPs@MnO2-AgNP nanocomposite was successfully applied for cancer theranostics. The fluorescence recognition of cancer was achieved by overexpressing GSH in cancer, meanwhile the photothermal therapy from CPs and chemotherapy from AgNPs jointly produced an enhanced therapeutic effect. This redox-responsive nanocomposite of CPs@MnO2-AgNPs improves the MnO2 nanomaterial-based applications in GSH sensing and cancer theranostics.


Assuntos
Glutationa/metabolismo , Compostos de Manganês , Nanopartículas Metálicas , Nanocompostos , Neoplasias , Imagem Óptica , Óxidos , Prata , Nanomedicina Teranóstica , Linhagem Celular Tumoral , Humanos , Compostos de Manganês/química , Compostos de Manganês/farmacologia , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Nanocompostos/química , Nanocompostos/uso terapêutico , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Neoplasias/metabolismo , Óxidos/química , Óxidos/farmacologia , Prata/química , Prata/farmacologia
5.
Nanoscale ; 11(39): 18209-18223, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31560010

RESUMO

Rheumatoid arthritis (RA) is a degenerative joint disease caused by autoimmunity; for the effective treatment of RA while avoiding the side effects of conventional drugs, we have proposed a new therapeutic strategy to eliminate the inflammatory response in RA by regulating the immune system that promotes the transformation of M1-type macrophages to M2-type macrophages. Herein, we designed and synthesized a core-shell nanocomposite (QRu-PLGA-RES-DS NPs), which showed an effective therapeutic effect on RA by accurately inducing the polarization of M2 macrophages. In this system, the quadrilateral ruthenium nanoparticles (QRuNPs) with a photothermal effect were utilized as a core and the thermosensitive molecular poly (lactic-co-glycolic acid) (PLGA) modified with the targeted molecule dextran sulfate (DS) was employed as a shell. Then, the nanocarrier QRu-PLGA-DS NPs effectively improved the water solubility and targeting of resveratrol (RES) through self-assembly. Therefore, the QRu-PLGA-RES-DS NPs significantly enhanced the ability of RES to reverse the M1 type macrophages to the M2 type macrophages through an accurate release. In vivo experiments further demonstrated that the QRu-PLGA-RES-DS NPs could effectively accumulate in the lesion area with an exogenous stimulus, and this significantly enhanced the transformation of the M2 type macrophages and decreased the recruitment of the M1 type macrophages. Furthermore, the QRu-PLGA-RES-DS NPs effectively treated RA by eliminating the inflammatory response; in addition, photoacoustic imaging (PA) of the QRu NPs provided image guidance for the distribution and analysis of nanomedicine in inflammatory tissues. Hence, this therapeutic strategy promotes the biological applications of Ru-based nanoparticles in disease treatment.


Assuntos
Hipertermia Induzida , Macrófagos/metabolismo , Nanocompostos , Fototerapia , Resveratrol , Febre Reumática/terapia , Animais , Células Endoteliais da Veia Umbilical Humana , Humanos , Macrófagos/patologia , Camundongos , Nanocompostos/química , Nanocompostos/uso terapêutico , Ácido Poliglicólico/química , Ácido Poliglicólico/farmacocinética , Ácido Poliglicólico/farmacologia , Células RAW 264.7 , Resveratrol/farmacocinética , Resveratrol/farmacologia , Febre Reumática/metabolismo , Febre Reumática/patologia , Rutênio/química , Rutênio/farmacocinética , Rutênio/farmacologia
6.
Mater Sci Eng C Mater Biol Appl ; 105: 110055, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546416

RESUMO

Designing of mechanically tough elastomeric materials encompassed with intrinsic surface hydrophobicity, antistatic and antimicrobial attributes is in skyrocketing demands, especially to protect the instruments which are submerged in water. Herein, the authors depicted the fabrication of interpenetrating polymer network-based nanocomposites containing different doses of octadecylamine capped Cu/RGO nanohybrid. The structures and morphologies of the synthesized nanohybrid and the fabricated nanocomposites were characterized by using FTIR, XRD, XPS, TGA, FESEM and TEM analyses. Most interestingly the nanocomposites showed good hydrophobicity (static contact angle: 119.2°-129.3°), low surface resistivity (~107 Ω m) and strong antimicrobial activity towards Gram negative (Pseudomonas aeruginosa and Yersinia pestis) and Gram positive (Bacillus cereus) bacterial strains. The fabricated nanocomposites also exhibited antifungal (Candida albicans) activity. In addition, the fabricated nanocomposites showed excellent mechanical properties including high tensile strength (14.03-20.9 MPa), outstanding flexibility (1887-2470%), excellent toughness (249.89-510.1 MJ.m-3), high scratch resistance (>10 kg) and high thermostability (281-288 °C). Therefore, the fabricated nanocomposites can be used as an effective thin film for many advanced applications.


Assuntos
Aminas , Anti-Infecciosos , Substâncias Antieletricidade Estática , Bactérias/crescimento & desenvolvimento , Candida albicans/crescimento & desenvolvimento , Cobre , Grafite , Nanocompostos , Aminas/química , Aminas/farmacologia , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Substâncias Antieletricidade Estática/química , Substâncias Antieletricidade Estática/farmacologia , Cobre/química , Cobre/farmacologia , Grafite/química , Grafite/farmacologia , Interações Hidrofóbicas e Hidrofílicas , Nanocompostos/química , Nanocompostos/uso terapêutico , Oxirredução
7.
Molecules ; 24(18)2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31546842

RESUMO

In thrombolytic therapy, plasminogen activators (PAs) are still the only group of drug approved to induce thrombolysis, and therefore, critical for treatment of arterial thromboembolism, such as stroke, in the acute phase. Functionalized nanocomposites have attracted great attention in achieving target thrombolysis due to favorable characteristics associated with the size, surface properties and targeting effects. Many PA-conjugated nanocomposites have been prepared and characterized, and some of them has been demonstrated with therapeutic efficacy in animal models. To facilitate future translation, this paper reviews recent progress of this area, especially focus on how to achieve reproducible thrombolysis efficacy in vivo.


Assuntos
Sistemas de Liberação de Medicamentos , Ativadores de Plasminogênio/farmacologia , Terapia Trombolítica , Animais , Modelos Animais de Doenças , Humanos , Nanocompostos/uso terapêutico
8.
ACS Appl Mater Interfaces ; 11(38): 34707-34716, 2019 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-31482705

RESUMO

It is of great significance to develop biocompatible and degradable gene carriers with stimuli-enhanced gene therapy and imaging function. In this work, low-cytotoxic polycation PGEA (ethanolamine-functionalized poly(glycidyl methacrylate))-functionalized dextran-quantum dot (QD) nanohybrids (DQ-PGEA) were proposed as safe and efficient gene carriers via a facile and feasible method. The highly water-soluble dextran gives the carrier good stability, biocompatibility, and abundant modification sites, while QDs allow fluorescence (FL) imaging. Taking advantage of the pH-responsive self-destruction characteristic introduced by Schiff base linkages, DQ-PGEA nanohybrids could not only result in enhanced gene release but also contribute to the elimination of the carriers. Reduced (nondegradable) DQ-PGEA-R nanohybrids were also synthesized as counterparts to reveal the superiority of the responsive DQ-PGEA carriers. The effectiveness of the as-prepared gene delivery systems was verified adopting the antioncogene p53 in the mouse model of breast cancer. As expected, DQ-PGEA nanohybrids demonstrated a superior gene transfection performance and antitumor inhibition compared with their counterparts. Meanwhile, the gene delivery processes could be tracked in real time to visualize the therapeutic processes and realize FL imaging-guided gene therapy. The current multifunctional stimuli-responsive nanoplatforms with the self-destruction feature are intriguing candidates to achieve enhanced gene therapy for tumor treatment.


Assuntos
Dextranos , Técnicas de Transferência de Genes , Terapia Genética , Neoplasias Mamárias Experimentais , Nanocompostos , Pontos Quânticos , Proteína Supressora de Tumor p53 , Animais , Linhagem Celular Tumoral , Dextranos/química , Dextranos/farmacologia , Células HEK293 , Humanos , Concentração de Íons de Hidrogênio , Neoplasias Mamárias Experimentais/genética , Neoplasias Mamárias Experimentais/metabolismo , Neoplasias Mamárias Experimentais/patologia , Neoplasias Mamárias Experimentais/terapia , Camundongos , Nanocompostos/química , Nanocompostos/uso terapêutico , Ácidos Polimetacrílicos/química , Ácidos Polimetacrílicos/farmacologia , Pontos Quânticos/química , Pontos Quânticos/uso terapêutico , Proteína Supressora de Tumor p53/biossíntese , Proteína Supressora de Tumor p53/genética
9.
Nanoscale ; 11(32): 14993-15003, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31380525

RESUMO

Quantum dots (QDs) have extensive application prospects in the fields of optics, magnetism, catalysis, and biomedicine. New carbon-doped QDs are currently being used in these research fields. Graphitic carbon nitride QDs (g-CNs) composed of only carbon and nitrogen have attracted attention because of their unique optical and catalytic properties. g-CNs have numerous electronic properties and can be used as photocatalytic modifiers in a wide range of applications in electrochemistry. Additionally, g-CNs also have biological potential and due to their chemical composition have extremely low toxicity; their blue light emission can be applied to biological imaging, and their appropriate energy level (2.7 eV) allows electrons to be deposited on their surface, which allows g-CNs to be used as photosensitizers in optical therapy. Finally, g-CNs can be combined with other nanomaterials to form composite materials, which can result in new advantages not seen in either of the materials alone. In this manuscript, we thoroughly report the most recent findings regarding the synthesis of g-CNs and their respective properties. We report the advantages of g-CNs conferred by their unique properties and their advantages for application in current biology and medicines.


Assuntos
Grafite/química , Nanocompostos/química , Compostos de Nitrogênio/química , Animais , Técnicas Biossensoriais/métodos , Humanos , Nanocompostos/uso terapêutico , Neoplasias/tratamento farmacológico , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Pontos Quânticos/química , Cicatrização/efeitos dos fármacos
10.
Int J Nanomedicine ; 14: 4529-4539, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31417256

RESUMO

Purpose: Here, we fabricated two plasmonic 2D Ti3C2Tx-based nanocomposites (Au/MXene and Au/Fe3O4/MXene) with similarly high anti-cancer photothermal therapy (PTT) capabilities, but with less in vivo toxicity than a pure MXene. Methods: Au/MXene was synthesized by in situ reduction of tetrachloroauric acid using NaBH4 on Ti3C2Tx flakes. For targeted PTT, magnetic Au/Fe3O4/MXene was synthesized via a reaction between freshly prepared magnetite Fe3O4 NPs and MXene solution, followed by in situ integration of gold nanoparticles (AuNPs). Results: Morphological characterization by XRD, SEM, and TEM revealed the successful synthesis of Au/MXene and Au/Fe3O4/MXene. Both new composites exhibited a significant in vitro dose-dependent PTT effect against human breast cancer cells MCF7. Interestingly, in vivo acute toxicity assays using zebrafish embryos indicated that Au/MXene and Au/Fe3O4/MXene had less embryonic mortality (LC50 ≫ 1000 µg/mL) than pure MXene (LC50=257.46 µg/mL). Conclusion: Our new Au/MXene and Au/Fe3O4/MXene nanocomposites could be safer and more suitable than the pure MXene for biomedical applications, especially when targeted PTT is warranted.


Assuntos
Hipertermia Induzida , Nanocompostos/uso terapêutico , Fototerapia , Titânio/química , Testes de Toxicidade Aguda , Animais , Sobrevivência Celular/efeitos dos fármacos , Embrião não Mamífero/efeitos dos fármacos , Humanos , Células MCF-7 , Nanocompostos/ultraestrutura , Teratogênios/toxicidade , Difração de Raios X , Peixe-Zebra
11.
ACS Appl Mater Interfaces ; 11(37): 33725-33733, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31449386

RESUMO

Wound healing is a dynamic, interactive, and complex process, including multiple stages. Although various nanomaterials are applied to accelerate the wound healing process through exhibiting antibacterial activity or promoting cell proliferation, only a single stage is promoted during the process, lowering healing efficacy. It is necessary to develop programmable nanosystems for promoting multiple wound healing stages in sequence. Herein, arginine-loaded and detachable ceria-graphene nanocomposites (ACG NCs) were designed to achieve this purpose. Ceria NPs and graphene were linked by base-cleavable N-hydroxysuccinimide ester. At inflammation stage, ACG NCs could effectively generate reactive oxygen species (ROS) and kill bacteria under white light irradiation due to their efficient electron-hole separation between ceria NPs and graphene. At proliferation stage, ceria NPs could be detached from ACG NCs and taken up by cells to scarify intracellular ROS and promote cell proliferation, while the separated graphene could act as a scaffold to promote fibroblast migration to wound site. A series of in vitro and in vivo assessments demonstrated that ACG NCs could effectively accelerate wound healing process.


Assuntos
Proliferação de Células/efeitos dos fármacos , Cério , Fibroblastos/metabolismo , Nanocompostos , Nanopartículas , Cicatrização/efeitos dos fármacos , Células 3T3 , Animais , Arginina/química , Arginina/farmacologia , Cério/química , Cério/farmacologia , Fibroblastos/patologia , Grafite/química , Grafite/farmacologia , Camundongos , Nanocompostos/química , Nanocompostos/uso terapêutico , Nanopartículas/química , Nanopartículas/uso terapêutico , Células RAW 264.7 , Espécies Reativas de Oxigênio/metabolismo
12.
J Colloid Interface Sci ; 556: 258-265, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31450020

RESUMO

Layered double hydroxide nanoparticles (LDHs) have shown the excellent capability and good adjuvant function as a nanocarrier for protein antigen delivery to enhance the immune response. Furthermore, LDHs have good biocompatibility and low cytotoxicity. However, their oral vaccine delivery efficiency is limited due to acidic/enzyme degradation in the stomach and low bioavailability in the small intestine. To overcome these challenges, alginate-chitosan coated LDHs nanocomposites (ALG-CHT-LDH) have been developed and used as a carrier for oral protein vaccine delivery. The physicochemical properties of ALG-CHT-LDH have been determined by dynamic light scattering (DLS), transmission electron microscopy (TEM), and ultraviolet visible (UV-Vis) spectroscopy. Protein release properties of LDHs with/without polymer coating have been investigated at various pHs. The protein release profile of ALG-CHT-LDH nanocomposites indicated that ALG-CHT coating could partially protect protein release at the acidic condition (pH 1.2). The cellular uptake efficiency of protein delivered by ALG-CHT-LDH for the intestine cells and macrophages were studied. After alginate layer falls from ALG-CHT-LDH nanocomposite, flow cytometry analysis (FACS) data suggest that chitosan-coated LDHs significantly enhance the internalization of proteins at the Caco2 and macrophage cells.


Assuntos
Alginatos , Quitosana , Portadores de Fármacos , Hidróxidos , Nanocompostos , Vacinas , Administração Oral , Alginatos/química , Alginatos/farmacologia , Animais , Células CACO-2 , Quitosana/química , Quitosana/farmacologia , Portadores de Fármacos/química , Portadores de Fármacos/farmacologia , Células HT29 , Humanos , Hidróxidos/química , Hidróxidos/farmacologia , Camundongos , Nanocompostos/química , Nanocompostos/uso terapêutico , Células RAW 264.7 , Vacinas/química , Vacinas/farmacologia
13.
ACS Appl Mater Interfaces ; 11(37): 34268-34281, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31454217

RESUMO

A multifunctional diagnosis and treatment integration platform is crucial in cancer treatments. Here, we show that by integrating Gd-doped silicon nanoparticles (Si-Gd NPs), chlorine e6 (Ce6), doxorubicin (DOX), zeolitic imidazolate framework-8 (ZIF-8), poly(2-(diethylamino)ethyl methacrylate) polymers (HOOC-PDMAEMA-SH), and folic acid-poly(ethylene glycol)-maleimide (MaL-PEG-FA) into one single nanoplatform by a self-assembly method, novel multifunctional MOFs (named FZIF-8/DOX-PD-FA) are synthesized with great biocompatibility and tumor targeting as well as pH responsiveness and no drug leakage for drug delivery. In the design, Si-Gd NPs and Ce6 embedded in the nanocomposites are used for magnetic resonance and fluorescence dual-modal imaging, respectively. DOX loaded by the FZIF-8/DOX-PD-FA porous structure is used for chemotherapy, while Ce6 is excited by near-infrared radiation (NIR) for photodynamic therapy. In addition, the pH-responsive ability of HOOC-PDMAEMA-SH to effectively prevent drug leakage is demonstrated by drug release studies in vitro. From the results of confocal microscopy imaging in vitro and fluorescence/magnetic resonance imaging in vivo, FZIF-8/DOX-PD-FA showed a targeting effect on MCF-7 cancer cells. More importantly, the results of treatment experiments on tumor-bearing mice showed that the tumor volume of the FZIF-8/DOX-PD-FA + NIR group is decreased the most compared to the original volume. Owing to the unique dual-modal imaging capability and excellent chemo-/photodynamic combinational cancer therapy effect, the present hybrid nanocarrier provides a new research platform for a new generation of theranostic nanoparticles.


Assuntos
Doxorrubicina , Portadores de Fármacos , Imagem por Ressonância Magnética , Nanocompostos , Neoplasias Experimentais , Imagem Óptica , Fotoquimioterapia , Células A549 , Animais , Doxorrubicina/química , Doxorrubicina/farmacocinética , Doxorrubicina/farmacologia , Portadores de Fármacos/química , Portadores de Fármacos/farmacocinética , Portadores de Fármacos/farmacologia , Humanos , Concentração de Íons de Hidrogênio , Células MCF-7 , Camundongos , Camundongos Nus , Nanocompostos/química , Nanocompostos/uso terapêutico , Neoplasias Experimentais/diagnóstico por imagem , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Experimentais/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
14.
ACS Appl Mater Interfaces ; 11(35): 31671-31680, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31397149

RESUMO

Hypoxia and overexpression of glutathione (GSH) are typical characteristics of the tumor microenvironment, which severely hinders cancer treatments. Here, we design a novel biodegradable therapeutic system, O2-Cu/ZIF-8@Ce6/ZIF-8@F127 (OCZCF), to simultaneously achieve GSH depletion and O2-enhanced combination therapy. Notably, the doped Cu2+ doubles the O2 storage capacity of the ZIF-8 matrix, which makes OCZCF an excellent pH-sensitive O2 reservoir for conquering tumor hypoxia, enhancing the photodynamic therapy (PDT) efficiency of chlorin e6 (Ce6) under 650 nm laser irradiation. Moreover, the released Cu2+ can act as a smart reactive oxygen species protector by consuming intracellular GSH. The byproduct Cu+ will undergo highly efficient Fenton-like reaction to achieve chemodynamic therapy (CDT) in the presence of abundant H2O2. The accompanying O2 will further alleviate hypoxia. The in vitro and in vivo experimental data indicate that OCZCF could cause remarkable tumor inhibition through enhanced synergetic PDT and CDT, which may open up a new path for cancer therapy.


Assuntos
Nanocompostos , Neoplasias Experimentais , Fotoquimioterapia , Polietilenos , Polipropilenos , Porfirinas , Microambiente Tumoral/efeitos dos fármacos , Animais , Linhagem Celular Tumoral , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacocinética , Preparações de Ação Retardada/farmacologia , Feminino , Camundongos , Camundongos Endogâmicos BALB C , Nanocompostos/química , Nanocompostos/uso terapêutico , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Experimentais/metabolismo , Neoplasias Experimentais/patologia , Polietilenos/química , Polietilenos/farmacocinética , Polietilenos/farmacologia , Polipropilenos/química , Polipropilenos/farmacocinética , Polipropilenos/farmacologia , Porfirinas/química , Porfirinas/farmacocinética , Porfirinas/farmacologia
15.
ACS Appl Mater Interfaces ; 11(37): 33535-33547, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31369233

RESUMO

Engineering bioscaffolds for improved cutaneous tissue regeneration remains a healthcare challenge because of the increasing number of patients suffering from acute and chronic wounds. To help address this problem, we propose to utilize alfalfa, an ancient medicinal plant that contains antibacterial/oxygenating chlorophylls and bioactive phytoestrogens, as a building block for regenerative wound dressings. Alfalfa carries genistein, which is a major phytoestrogen known to accelerate skin repair. The scaffolds presented herein were built from composite alfalfa and polycaprolactone (PCL) nanofibers with hydrophilic surface and mechanical stiffness that recapitulate the physiological microenvironments of skin. This composite scaffold was engineered to have aligned nanofibrous architecture to accelerate directional cell migration. As a result, alfalfa-based composite nanofibers were found to enhance the cellular proliferation of dermal fibroblasts and epidermal keratinocytes in vitro. Finally, these nanofibers exhibited reproducible regenerative functionality by promoting re-epithelialization and granulation tissue formation in both mouse and human skin, without requiring additional proteins, growth factors, or cells. Overall, these findings demonstrate the potential of alfalfa-based nanofibers as a regenerative platform toward accelerating cutaneous tissue repair.


Assuntos
Derme , Queratinócitos , Medicago sativa/química , Nanocompostos , Nanofibras , Cicatrização/efeitos dos fármacos , Linhagem Celular , Derme/lesões , Derme/metabolismo , Derme/patologia , Humanos , Queratinócitos/metabolismo , Queratinócitos/patologia , Nanocompostos/química , Nanocompostos/uso terapêutico , Nanofibras/química , Nanofibras/uso terapêutico , Poliésteres/química
16.
ACS Appl Mater Interfaces ; 11(28): 24959-24970, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31265222

RESUMO

Inorganic nanomaterial (INM)-based combination cancer therapies have been extensively employed over the past two decades because of their benefits over traditional chemo- and radiotherapies. However, issues regarding the toxicity and accumulation of INMs in the body have arisen. This problem may be improved through the use of biodegradable or disintegrable nanosystems such as black phosphorus (BP). Challenges to the manufacture of fully nanodimensional BP remain. In addition, improvements in recently developed cancer immunotherapies require their incorporation with drugs, targeting agents, and delivery vehicles. With these needs in mind, this study develops a method for instant in-flight manufacture of nanodimensional BP using plug-and-play devices for subsequent assembly of photoimmunotherapeutic core@shell composites containing mutated B-raf inhibitors (dabrafenib), immune checkpoint inhibitors (PD-L1), and cancer-targeting antibodies (CXCR4). The resulting nanocomposites exhibited cancer targetability and regulatability of PD-L1 expression both in vitro and in vivo. These activities were further increased upon near-infrared irradiation due to the incorporation of a phototherapeutic component. These results suggest that these nanocomposites are promising as a new class of advanced cancer therapeutic agents.


Assuntos
Antineoplásicos , Imidazóis , Imunoterapia , Melanoma Experimental/terapia , Nanocompostos , Oximas , Fototerapia , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Imidazóis/química , Imidazóis/farmacologia , Melanoma Experimental/imunologia , Melanoma Experimental/patologia , Camundongos , Nanocompostos/química , Nanocompostos/uso terapêutico , Oximas/química , Oximas/farmacologia
17.
J Cancer Res Clin Oncol ; 145(9): 2199-2209, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31309302

RESUMO

PURPOSE: Radiofrequency (RF) ablation therapy is of great interest in cancer therapy as it is non-ionizing radiation and can effectively penetrate into the tissue. However, the current RF ablation technique is invasive that requires RF probe insertion into the tissue and generates a non-specific heating. Recently, RF-responsive nanomaterials such as gold nanoparticles (AuNPs) and iron oxide nanoparticles (IONPs) have led to tremendous progress in this area. They have been found to be able to absorb the RF field and induce a localized heating within the target, thereby affording a non-invasive and tumor-specific RF ablation strategy. In the present study, for the first time, we used a hybrid core-shell nanostructure comprising IONPs as the core and AuNPs as the shell (IO@Au) for targeted RF ablation therapy. Due to the magnetic core, the nanohybrid can be directed toward the tumor through a magnet. Moreover, IONPs enable the nanohybrid to be used as a magnetic resonance imaging (MRI) contrast agent. RESULTS: In vitro cytotoxicity experiment showed that the combination of IO@Au and 13.56-MHz RF field significantly reduced the viability of cancer cells. Next, during an in vivo experiment, we demonstrated that magnetically targeting of IO@Au to the tumor and subsequent RF exposure dramatically suppressed the tumor growth. CONCLUSION: Therefore, the integration of targeting, imaging, and therapeutic performances into IO@Au nanohybrid could afford the promise to improve the effectiveness of RF ablation therapy.


Assuntos
Ablação por Cateter/métodos , Compostos Férricos/química , Ouro/química , Hipertermia Induzida/métodos , Nanopartículas de Magnetita/uso terapêutico , Neoplasias/cirurgia , Ablação por Radiofrequência/métodos , Animais , Compostos Férricos/uso terapêutico , Ouro/uso terapêutico , Imagem por Ressonância Magnética/métodos , Nanopartículas de Magnetita/química , Masculino , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Camundongos , Camundongos Endogâmicos BALB C , Terapia de Alvo Molecular/métodos , Nanocompostos/química , Nanocompostos/uso terapêutico , Nanoconchas/química , Nanoconchas/uso terapêutico , Neoplasias/patologia , Células Tumorais Cultivadas
18.
ACS Appl Mater Interfaces ; 11(35): 31615-31626, 2019 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-31359757

RESUMO

Phototherapy, including photothermal and photodynamic therapy, has attracted extensive attention due to its noninvasive nature, low toxicity, and high anticancer efficiency. The charge-separation mechanism of plasmon-induced resonance energy transfer (PIRET) has been increasingly employed to design nanotheranotic agents. Herein, we developed a novel and smart PIRET-mediated nanoplatform for enhanced, imaging-guided phototherapy. Prussian blue (PB) was incorporated into a Au@Cu2O nanostructure, which was then assembled with poly(allylamine) (PAH)-modified black phosphorus quantum dots (Au@PB@Cu2O@BPQDs/PAH nanocomposites). The hybrid nanosystem exhibited great absorption in the near-infrared region, as well as the ability to self-supply O2 by catalyzing hydrogen peroxide and convert O2 into singlet oxygen (1O2) under 650 nm laser light (0.5 W/cm2) irradiation. In vitro and in vivo assays showed that the generated heat and toxic 1O2 from Au@PB@Cu2O@BPQDs/PAH nanocomposites could effectively kill the cancer cells and suppress tumor growth. Moreover, the unique properties of the PB-modified nanosystem allowed for synergistic therapy with the aid of T1-weighed magnetic resonance imaging (T1-weighted magnetic resonance imaging) and photoacoustic imaging. This study presented a suitable way to fabricate smart PIRET-based nanosystems with enhanced photothermal therapy/photodynamic therapy efficacy and dual-modality imaging functionality. The great biocompatibility and low toxicity ensured their high potential for use in cancer therapy.


Assuntos
Hipertermia Induzida , Imagem por Ressonância Magnética , Nanocompostos , Neoplasias Experimentais , Técnicas Fotoacústicas , Fototerapia , Pontos Quânticos , Animais , Cobre/química , Cobre/farmacologia , Feminino , Ferrocianetos/química , Ferrocianetos/farmacologia , Ouro/química , Ouro/farmacologia , Células HeLa , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Nanocompostos/química , Nanocompostos/uso terapêutico , Neoplasias Experimentais/diagnóstico por imagem , Neoplasias Experimentais/metabolismo , Neoplasias Experimentais/terapia , Pontos Quânticos/química , Pontos Quânticos/uso terapêutico , Ensaios Antitumorais Modelo de Xenoenxerto
19.
Nanoscale ; 11(34): 15685-15708, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31355405

RESUMO

Two-dimensional (2D) nanocomposites have been widely used in biomedical applications during the past few years due to their extraordinary physicochemical properties, which has proved their importance in the field of nanomedicine. Benefiting from the excellent optical absorption in the near-infrared window and large specific surface area, many efforts have been devoted to fabricating 2D nanomaterial-based multifunctional nanoplatforms to realize photothermal therapy (PTT)-based or chemotherapy-based synergistic treatment, which exhibits obvious anti-tumor effects and significantly enhances the therapeutic efficiency of cancer compared with monotherapy. In particular, 2D nanocomposites are usually fabricated as intelligent nanoplatforms for stimuli-responsive nanocarriers, whose therapeutic effects could be specifically activated by the tumor microenvironment (TME). In addition, different fluorescent probes and functional inorganic nanomaterials could be absorbed on the surface of 2D nanomaterials to fabricate multifunctional hybrid nanomaterials with satisfactory magnetic, optical, or other properties that are widely used for multimodal imaging-guided cancer therapy. In this review, the latest development of multifunctional 2D nanocomposites for combination therapy is systematically summarized, mainly focusing on PTT-based synergistic cancer therapy, and the other forms and potential forms of synergistic cancer therapy are also simply summarized. Furthermore, the design principles of 2D nanocomposites are particularly emphasized, and the current challenges and future prospects of 2D nanocomposites for cancer theranostics are discussed simultaneously.


Assuntos
Hipertermia Induzida , Nanocompostos , Neoplasias/terapia , Fototerapia , Nanomedicina Teranóstica , Humanos , Nanocompostos/química , Nanocompostos/uso terapêutico , Neoplasias/metabolismo , Neoplasias/patologia
20.
Nanoscale ; 11(24): 11470-11483, 2019 Jun 20.
Artigo em Inglês | MEDLINE | ID: mdl-31124554

RESUMO

Sonochemotherapy is a promising strategy for inhibiting tumor growth. However, achieving highly targeted and effective sonochemotherapy is still an enormous challenge. In this study, a novel chemotherapeutic-carrying nanocomposite (HPCID) was developed, which can effectively target metastatic cancer cells and provide an enhanced therapeutic effect. In detail, HPCID was composed of hyaluronic acid (HA), carboxyl-terminated PAMAM dendrimer, fluorochrome indocyanine green (ICG), and doxorubicin hydrochloride (Dox). The efficacy of this drug delivery system (DDS) in sonochemotherapy was assessed on the CD44-overexpressing metastatic breast cancer cell line 4T1 both in vitro and in vivo. The HA modification significantly improved the cellular internalization of HPCID, and the degradation of the HA shell by hyaluronidase that is abundant in the 4T1 cells resulted in enzyme-responsive drug release. Under ultrasound (US) stimulation, HPCID produced a high amount of reactive oxidant species (ROS), which induced significant cell apoptosis when combined with chemotherapy. In addition, the administration of HPCID in 4T1 xenograft-bearing mice combined with ultrasonic exposure significantly inhibited tumor growth and pulmonary metastasis, with no systemic toxicity. Taken together, the proposed HPCID-mediated sonodynamic therapy (SDT) is a novel strategy against breast cancer progression and metastasis.


Assuntos
Doxorrubicina , Sistemas de Liberação de Medicamentos , Ácido Hialurônico , Neoplasias Mamárias Experimentais/terapia , Nanocompostos , Terapia por Ultrassom , Animais , Doxorrubicina/química , Doxorrubicina/farmacologia , Feminino , Ácido Hialurônico/química , Ácido Hialurônico/farmacologia , Neoplasias Mamárias Experimentais/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Nanocompostos/química , Nanocompostos/uso terapêutico , Metástase Neoplásica
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