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1.
Int J Mol Sci ; 22(14)2021 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-34299003

RESUMO

Here, we designed paper sheets coated with chitosan, bacterial cellulose (nanofibers), and ZnO with boosted antibacterial and mechanical activity. We investigated the compositions, with ZnO exhibiting two different sizes/shapes: (1) rods and (2) irregular sphere-like particles. The proposed processing of bacterial cellulose resulted in the formation of nanofibers. Antimicrobial behavior was tested using E. coli ATCC® 25922™ following the ASTM E2149-13a standard. The mechanical properties of the paper sheets were measured by comparing tearing resistance, tensile strength, and bursting strength according to the ISO 5270 standard. The results showed an increased antibacterial response (assigned to the combination of chitosan and ZnO, independent of its shape and size) and boosted mechanical properties. Therefore, the proposed composition is an interesting multifunctional mixture for coatings in food packaging applications.


Assuntos
Biopolímeros/química , Biopolímeros/farmacologia , Celulose/química , Quitosana/química , Nanocompostos/química , Embalagem de Produtos/métodos , Óxido de Zinco/química , Anti-Infecciosos , Celulose/ultraestrutura , Escherichia coli , Testes Mecânicos , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Nanocompostos/ultraestrutura , Nanofibras/química , Nanofibras/ultraestrutura , Propriedades de Superfície , Resistência à Tração , Difração de Raios X
2.
Life Sci ; 282: 119602, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34217765

RESUMO

The application of electroactive scaffolds can be promising for bone tissue engineering applications. In the current paper, we aimed to fabricate an electro-conductive scaffold based on carbon nanofibers (CNFs) containing ferrous sulfate. FeSO4·7H2O salt with different concentrations 5, 10, and 15 wt%, were blended with polyacrylonitrile (PAN) polymer as the precursor and converted to Fe2O3/CNFs nanocomposite by electrospinning and heat treatment. The characterization was conducted using SEM, EDX, XRD, FTIR, and Raman methods. The results showed that the incorporation of Fe salt induces no adverse effect on the nanofibers' morphology. EDX analysis confirmed that the Fe ions are uniformly dispersed throughout the CNF mat. FTIR spectroscopy showed the interaction of Fe salt with PAN polymer. Raman spectroscopy showed that the incorporation of FeSO4·7H2O reduced the ID/IG ratio, indicating more ordered carbon in the synthesized nanocomposite. Electrical resistance measurement depicted that, although the incorporation of ferrous sulfate reduced the electrical conductivity, the conductive is suitable for electrical stimulation. The in vitro studies revealed that the prepared nanocomposites were cytocompatible and only negligible toxicity (less than 10%) induced by CNFs/Fe2O3 fabricated from PAN FeSO4·7H2O 15%. Although various nanofibrous composite fabricated with Fe NPs have been evaluated for tissue engineering applications, CNFs exhibited promising properties, such as excellent mechanical strength, biocompatibility, and electrical conductivity. These results showed that the fabricated nanocomposites could be applied as the bone tissue engineering scaffold.


Assuntos
Osso e Ossos/citologia , Carbono/química , Compostos Ferrosos/química , Nanofibras/química , Engenharia Tecidual/métodos , Tecidos Suporte/química , Linhagem Celular , Proliferação de Células , Condutividade Elétrica , Humanos , Nanofibras/ultraestrutura
3.
Nat Commun ; 12(1): 4494, 2021 07 23.
Artigo em Inglês | MEDLINE | ID: mdl-34301935

RESUMO

Self-assembling peptides have shown tremendous potential in the fields of material sciences, nanoscience, and medicine. Because of the vast combinatorial space of even short peptides, identification of self-assembling sequences remains a challenge. Herein, we develop an experimental method to rapidly screen a huge array of peptide sequences for self-assembling property, using the one-bead one-compound (OBOC) combinatorial library method. In this approach, peptides on beads are N-terminally capped with nitro-1,2,3-benzoxadiazole, a hydrophobicity-sensitive fluorescence molecule. Beads displaying self-assembling peptides would fluoresce under aqueous environment. Using this approach, we identify eight pentapeptides, all of which are able to self-assemble into nanoparticles or nanofibers. Some of them are able to interact with and are taken up efficiently by HeLa cells. Intracellular distribution varied among these non-toxic peptidic nanoparticles. This simple screening strategy has enabled rapid identification of self-assembling peptides suitable for the development of nanostructures for various biomedical and material applications.


Assuntos
Nanofibras/química , Nanoestruturas/química , Biblioteca de Peptídeos , Peptídeos/química , Dicroísmo Circular , Técnicas de Química Combinatória/métodos , Células HeLa , Ensaios de Triagem em Larga Escala/métodos , Humanos , Ligação de Hidrogênio , Microscopia Eletrônica de Transmissão , Nanofibras/ultraestrutura , Nanoestruturas/ultraestrutura , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
4.
Int J Mol Sci ; 22(14)2021 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-34299308

RESUMO

In bone surgery and orthopedics, bioresorbable materials can be helpful in bone repair and countering post-op infections. Explicit antibacterial activity, osteoinductive and osteoconductive effects are essential to achieving this objective. Nonwoven electrospun (ES) fibers are receiving the close attention of physicians as promising materials for wound dressing and tissue engineering; potentially, in high contrast with dense materials, ES mats hamper regeneration of the bone extracellular matrix to a lesser extent. The use of the compositions of inherently biodegradable polyesters (poly(ε-caprolactone) PCL, poly(lactoglycolide), etc.), calcium phosphates and antibiotics is highly prospective, but the task of forming ES fibers from such compositions is complicated by the incompatibility of the main organic and inorganic ingredients, polyesters and calcium phosphates. In the present research we report the synthesis of hydroxyapatite (HAp) nanoparticles with uniform morphology, and demonstrate high efficiency of the block copolymer of PCL and poly(ethylene phosphoric acid) (PEPA) as an efficient compatibilizer for PCL/HAp mixtures that are able to form ES fibers with improved mechanical characteristics. The materials obtained in the presence of vancomycin exhibited incremental drug release against Staphylococcus aureus (St. aureus).


Assuntos
Antibacterianos/química , Substitutos Ósseos/química , Hidroxiapatitas/química , Antibacterianos/administração & dosagem , Fenômenos Biomecânicos , Liberação Controlada de Fármacos , Humanos , Técnicas In Vitro , Teste de Materiais , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Nanofibras/química , Nanofibras/ultraestrutura , Nanopartículas/química , Nanopartículas/ultraestrutura , Poliésteres/química , Polietilenos/química , Staphylococcus aureus/efeitos dos fármacos , Vancomicina/administração & dosagem , Vancomicina/química , Difração de Raios X
5.
Int J Mol Sci ; 22(11)2021 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-34071337

RESUMO

Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering. The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities. Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route. We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite. Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm. pH levels between 8-10 led to the formation of ultrafine ZnO particles with a uniform size distribution. The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF. The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria. A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR. The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.


Assuntos
Antibacterianos/química , Arecaceae/química , Celulose/química , Nanocompostos/química , Nanofibras/química , Óxido de Zinco/química , Antibacterianos/síntese química , Antibacterianos/farmacologia , Estabilidade de Medicamentos , Concentração de Íons de Hidrogênio , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento , Microscopia Eletrônica , Nanocompostos/ultraestrutura , Nanofibras/ultraestrutura , Tamanho da Partícula , Borracha/química , Salmonella/efeitos dos fármacos , Salmonella/crescimento & desenvolvimento , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Difração de Raios X
6.
Int J Mol Sci ; 22(11)2021 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-34070436

RESUMO

The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.


Assuntos
Modelos Animais de Doenças , Células-Tronco Mesenquimais/metabolismo , Nanofibras/química , Regeneração Nervosa , Neurogênese , Traumatismos dos Nervos Periféricos/terapia , Nervo Isquiático/metabolismo , Tecidos Suporte/química , Compostos de Anilina/química , Animais , Caproatos/química , Células Cultivadas , Colágeno/química , Imuno-Histoquímica , Lactonas/química , Masculino , Teste de Materiais , Células-Tronco Mesenquimais/citologia , Microscopia Eletrônica de Varredura , Músculo Esquelético/citologia , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Nanofibras/ultraestrutura , Traumatismos dos Nervos Periféricos/metabolismo , Traumatismos dos Nervos Periféricos/patologia , Poliésteres/química , Ratos , Ratos Endogâmicos Lew , Nervo Isquiático/citologia , Nervo Isquiático/patologia , Transplante Autólogo
7.
Int J Biol Macromol ; 182: 1392-1398, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34000313

RESUMO

One of the most important environmental issues in the world today is the problem of air pollution, which includes particulate matter (PM) and greenhouse gases (mainly CO2). The production of efficient sustainable filters to overcome this concern as well as to provide an alternative to synthetic petroleum-based filters remains a demanding challenge. The purpose of this research was to first produce novel cellulose nanofibers (CNF) based nanofilter from a combination of CNF and chitosan (CS) and then evaluate its applicability for air purification. A number of structural and chemical properties as well as CO2 and PM adsorption efficiency of the modified CNF, were determined using advanced characterization techniques. After pretests, we determined the optimum loading for the CS was 1 wt%, and upon producing the samples, the CNF loadings (1, 1.5, and 2 wt%) were chosen as one variable. For particle absorption, the PM sizes (0.1, 0.3, 0.5, and 2.5 µm) were kept as other variables. Based on SEM results, we concluded the higher the concentration of CNF the higher the specific surface area and the lower the porosity and the diameter of the pores, which was confirmed by the BET test. Furthermore, the results showed that increasing the concentration of modified CNFs increases the adsorption rate of CO2 and PM and that the highest adsorption of CO2 and PM belonged to the 2% modified CNF.


Assuntos
Ar , Celulose/química , Filtração/instrumentação , Nanofibras/química , Adsorção , Análise de Variância , Dióxido de Carbono/química , Celulose/ultraestrutura , Nanofibras/ultraestrutura , Material Particulado/análise , Porosidade , Pressão , Espectrometria por Raios X , Espectroscopia de Infravermelho com Transformada de Fourier
8.
Int J Mol Sci ; 22(8)2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33921095

RESUMO

Physically crosslinked hydrogels with thixotropic properties attract considerable attention in the biomedical research field because their self-healing nature is useful in cell encapsulation, as injectable gels, and as bioinks for three-dimensional (3D) bioprinting. Here, we report the formation of thixotropic hydrogels containing nanofibers of double-hydrophobic elastin-like polypeptides (ELPs). The hydrogels are obtained with the double-hydrophobic ELPs at 0.5 wt%, the concentration of which is an order of magnitude lower than those for previously reported ELP hydrogels. Although the kinetics of hydrogel formation is slower for the double-hydrophobic ELP with a cell-binding sequence, the storage moduli G' of mature hydrogels are similar regardless of the presence of a cell-binding sequence. Reversible gel-sol transitions are demonstrated in step-strain rheological measurements. The degree of recovery of the storage modulus G' after the removal of high shear stress is improved by chemical crosslinking of nanofibers when intermolecular crosslinking is successful. This work would provide deeper insight into the structure-property relationships of the self-assembling polypeptides and a better design strategy for hydrogels with desired viscoelastic properties.


Assuntos
Elastina/química , Hidrogéis/química , Interações Hidrofóbicas e Hidrofílicas , Nanofibras/química , Peptídeos/química , Sequência de Aminoácidos , Reagentes para Ligações Cruzadas/química , Módulo de Elasticidade , Iridoides/química , Nanofibras/ultraestrutura , Reologia
9.
Molecules ; 26(7)2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33916094

RESUMO

The degradation and mechanical properties of potential polymeric materials used for green manufacturing are significant determinants. In this study, cellulose nanofibre was prepared from Schizostachyum brachycladum bamboo and used as reinforcement in the PLA/chitosan matrix using melt extrusion and compression moulding method. The cellulose nanofibre(CNF) was isolated using supercritical carbon dioxide and high-pressure homogenisation. The isolated CNF was characterised with transmission electron microscopy (TEM), FT-IR, zeta potential and particle size analysis. The mechanical, physical, and degradation properties of the resulting biocomposite were studied with moisture content, density, thickness swelling, tensile, flexural, scanning electron microscopy, thermogravimetry, and biodegradability analysis. The TEM, FT-IR, and particle size results showed successful isolation of cellulose nanofibre using this method. The result showed that the physical, mechanical, and degradation properties of PLA/chitosan/CNF biocomposite were significantly enhanced with cellulose nanofibre. The density, thickness swelling, and moisture content increased with the addition of CNF. Also, tensile strength and modulus; flexural strength and modulus increased; while the elongation reduced. The carbon residue from the thermal degradation and the glass transition temperature of the PLA/chitosan/CNF biocomposite was observed to increase with the addition of CNF. The result showed that the biocomposite has potential for green and sustainable industrial application.


Assuntos
Materiais Biocompatíveis/química , Celulose/química , Quitosana/química , Nanocompostos/química , Nanofibras/química , Poliésteres/química , Sasa/química , Algoritmos , Fenômenos Químicos , Modelos Teóricos , Nanocompostos/ultraestrutura , Nanofibras/ultraestrutura , Tamanho da Partícula , Termogravimetria
10.
Molecules ; 26(9)2021 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-33925130

RESUMO

In this research, polyvinyl-alcohol (PVA)/gelatin (GEL)/propolis (Ps) biocompatible nanofiber patches were fabricated via electrospinning technique. The controlled release of Propolis, surface wettability behaviors, antimicrobial activities against the S. aureus and P. aeruginosa, and biocompatibility properties with the mesenchymal stem cells (MSCs) were investigated in detail. By adding 0.5, 1, and 3 wt.% GEL into the 13 wt.% PVA, the morphological and mechanical results suggested that 13 wt.% PVA/0.5 wt.% GEL patch can be an ideal matrix for 3 and 5 wt.% propolis addition. Morphological results revealed that the diameters of the electrospun nanofiber patches were increased with GEL (from 290 nm to 400 nm) and Ps addition and crosslinking process cause the formation of thicker nanofibers. The tensile strength and elongation at break enhancement were also determined for 13 wt.% PVA/0.5 wt.% GEL/3 wt.% Ps patch. Propolis was released quickly in the first hour and arrived at a plateau. Cell culture and contact angle results confirmed that the 3 wt.% addition of propolis reinforced mesenchymal stem cell proliferation and wettability properties of the patches. The antimicrobial activity demonstrated that propolis loaded patches had antibacterial activity against the S. aureus, but for P. aeruginosa, more studies should be performed.


Assuntos
Anti-Infecciosos/administração & dosagem , Materiais Biocompatíveis , Ceratite/tratamento farmacológico , Ceratite/microbiologia , Nanofibras , Própole/administração & dosagem , Materiais Biocompatíveis/química , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Testes de Sensibilidade Microbiana , Nanofibras/química , Nanofibras/ultraestrutura , Álcool de Polivinil/química , Própole/química , Pseudomonas aeruginosa/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Staphylococcus aureus/efeitos dos fármacos , Propriedades de Superfície
11.
Int J Mol Sci ; 22(8)2021 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-33924640

RESUMO

Essential oils prevent superbug formation, which is mainly caused by the continuous use of synthetic drugs. This is a significant threat to health, the environment, and food safety. Plant extracts in the form of essential oils are good enough to destroy pests and fight bacterial infections in animals and humans. In this review article, different essential oils containing polymeric nanofibers fabricated by electrospinning are reviewed. These nanofibers containing essential oils have shown applications in biomedical applications and as food-packaging materials. This approach of delivering essential oils in nanoformulations has attracted considerable attention in the scientific community due to its low price, a considerable ratio of surface area to volume, versatility, and high yield. It is observed that the resulting nanofibers possess antimicrobial, anti-inflammatory, and antioxidant properties. Therefore, they can reduce the use of toxic synthetic drugs that are utilized in the cosmetics, medicine, and food industries. These nanofibers increase barrier properties against light, oxygen, and heat, thereby protecting and preserving the food from oxidative damage. Moreover, the nanofibers discussed are introduced with naturally derived chemical compounds in a controlled manner, which simultaneously prevents their degradation. The nanofibers loaded with different essential oils demonstrate an ability to increase the shelf-life of various food products while using them as active packaging materials.


Assuntos
Embalagem de Alimentos , Nanofibras/química , Óleos Voláteis/química , Polímeros/química , Animais , Composição de Medicamentos , Humanos , Nanofibras/ultraestrutura , Tecidos Suporte/química
12.
J Mater Sci Mater Med ; 32(2): 21, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33649939

RESUMO

Increasing morbidity of cardiovascular diseases in modern society has made it crucial to develop artificial small-caliber cardiovascular grafts for surgical intervention of diseased natural arteries, as alternatives to the gold standard autologous implants. Synthetic small-caliber grafts are still not in use due to increased risk of restenosis, lack of lumen re-endothelialization and mechanical mismatch, leading sometimes either to graft failure or to unsuccessful remodeling and pathology of the distal parts of the anastomosed healthy vascular tissues. In this work, we aimed to synthesize small-caliber polymeric (polycaprolactone) tissue-engineered vascular scaffolds that mimic the structure and biomechanics of natural vessels. Electrospinning was implemented to prepare microstructured polymeric membranes with controlled axis-parallel fiber alignment. Consequently, we designed small-caliber multilayer anisotropic biodegradable nanofibrous tubular scaffolds, giving attention to their radial compliance. Polycaprolactone scaffold morphology and mechanical properties were assessed, quantified, and compared with those of native vessels and commercial synthetic grafts. Results showed a highly hydrophobic scaffold material with a three-layered tubular morphology, 4-mm internal diameter/0.25 ± 0.09-mm thickness, consisting of predominantly axially aligned thin (1.156 ± 0.447 µm), homogeneous and continuous microfibers, with adequate (17.702 ± 5.369 µm) pore size, potentially able to promote cell infiltration in vivo. In vitro accelerated degradation showed a 5% mass loss within 17-25 weeks. Mechanical anisotropy was attained as a result, almost one order of magnitude difference of the elastic modulus (18 ± 3 MPa axially/1 ± 0.3 MPa circumferentially), like that of natural arterial walls. Furthermore, a desirable radial compliance (5.04 ± 0.82%, within the physiological pressure range) as well as cyclic stability of the tubular scaffold was achieved. Finally, cytotoxicity evaluation of the polymeric scaffolds revealed that the materials were nontoxic and did not release substances harmful to living cells (over 80% cell viability achieved).


Assuntos
Implantes Absorvíveis , Prótese Vascular , Poliésteres/química , Engenharia Tecidual/métodos , Tecidos Suporte/química , Materiais Biocompatíveis/química , Fenômenos Biomecânicos , Linhagem Celular , Sobrevivência Celular , Complacência (Medida de Distensibilidade) , Módulo de Elasticidade , Células Endoteliais/citologia , Humanos , Teste de Materiais , Microscopia Eletrônica de Varredura , Nanofibras/química , Nanofibras/ultraestrutura , Suturas , Resistência à Tração , Engenharia Tecidual/instrumentação , Molhabilidade
13.
Int J Nanomedicine ; 16: 1361-1376, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33658777

RESUMO

Object: High targeting and efficient cytotoxicity toward tumor cells endow NPs excellent anti-tumor activity. Herein, a peptide polymer possessing dual-targeting ability and double therapeutic activity was developed and named TGMF, which can form NPs through self-assembly. It is composed of four functional modules: 1) Active targeting peptide TMTP1 (T) deliver NPs to tumors specifically; 2) Therapeutic peptide GO-203 (G), which can significantly inhibit tumor growth by disrupting the redox balance in cells; 3) A passively targeted enzyme-responsive peptide PLGLGA (M), which can be cleaved specifically by metalloproteinase-2 (MMP-2) highly expressed in the tumor microenvironment (TME); and 4) Hexadecyl (F), which has strong hydrophobicity, can promote the self-assembly of TGMF NPs. Methods: Five modular peptide probes, namely, TGF, TMF, TGM, GMF, and TGMF were synthesized and self-assembled into NPs in solution. The characterization, enzyme reactivity, and cytotoxicity of NPs were evaluated in vitro, and the pharmacokinetics, bio-distribution, anti-tumor activity of NPs were investigated in vivo. In addition, transcriptome sequencing identified the intracellular signaling pathway-related genes involved in the anti-tumor effect of TGMF. Results: Upon enzyme cleavage, two types of nanostructure, NPs and nanofibers (NFs), were detected under TEM. Moreover, the cytotoxicity and anti-invasion activity of TGMF against tumor cells used were strongest among the five modular probes examined in vitro. TGMF increased reactive oxygen species (ROS) levels in cytoplasm and produced numerous NFs in extracellular interval and intracellular space. Transcriptome sequencing revealed that TGMF caused 446 genes' down-regulation and 270 genes' up-regulation in HeLa cells. In vivo, TGMF has a good anti-tumor effect, effectively prolonging the survival time of HeLa-tumor-bearing mice without systemic side effects. Conclusion: Integration of multiple functional modules into NPs could be a promising strategy for the future of nanomedicine design towards tumor treatment.


Assuntos
Antineoplásicos/uso terapêutico , Nanopartículas/uso terapêutico , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Animais , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/genética , Feminino , Células HeLa , Humanos , Metaloproteinase 2 da Matriz/metabolismo , Camundongos Endogâmicos BALB C , Camundongos Nus , Nanofibras/química , Nanofibras/ultraestrutura , Nanopartículas/ultraestrutura , Invasividade Neoplásica , Metástase Neoplásica , Neoplasias/genética , Peptídeos/química , Polímeros/química , Análise de Componente Principal , Espécies Reativas de Oxigênio/metabolismo , Transcriptoma/efeitos dos fármacos , Transcriptoma/genética , Microambiente Tumoral/efeitos dos fármacos
14.
Int J Nanomedicine ; 16: 1789-1804, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33688191

RESUMO

Background: SARS-COVID-2 has recently been one of the most life-threatening problems which urgently needs new therapeutic antiviral agents, especially those of herbal origin. Purpose: The study aimed to load acaciin (ACA) into the new self-assembled nanofibers (NFs) followed by investigating their possible antiviral effect against bovine coronavirus (BCV) as a surrogate model for SARS-COV-2. Methods: ACA was identified using 1H-NMR and DEPT-Q 13C-NMR spectroscopy, the molecular docking study was performed using Autodock 4 and a modification of the traditional solvent injection method was applied for the synthesis of the biodegradable NFs. Different characterization techniques were used to inspect the formation of the NFs, which is followed by antiviral investigation against BCV as well as MTT assay using MDBK cells. Results: Core/shell NFs, ranging between 80-330 nm with tiny thorn-like branches, were formed which attained an enhanced encapsulation efficiency (97.5 ± 0.53%, P<0.05) and a dual controlled release (a burst release of 65% at 1 h and a sustained release up to >24 h). The antiviral investigation of the formed NFs revealed a significant inhibition of 98.88 ± 0.16% (P<0.05) with IC50 of 12.6 µM against BCV cells. Conclusion: The results introduced a new, time/cost-saving strategy for the synthesis of biodegradable NFs without the need for electric current or hazardous cross-linking agents. Moreover, it provided an innovative avenue for the discovery of drugs of herbal origin for the fight against SARS-CoV-2 infection.


Assuntos
Coronavirus Bovino/efeitos dos fármacos , Glicosídeos/farmacologia , Nanofibras/química , SARS-CoV-2/efeitos dos fármacos , Antivirais/química , Antivirais/farmacologia , COVID-19/tratamento farmacológico , COVID-19/virologia , Linhagem Celular , Glicosídeos/química , Glicosídeos/isolamento & purificação , Glicosídeos/uso terapêutico , Humanos , Ligantes , Modelos Biológicos , Simulação de Acoplamento Molecular , Nanofibras/ultraestrutura , Solventes , Raios Ultravioleta
15.
Small ; 17(12): e2100139, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33656273

RESUMO

The novel coronavirus SARS-CoV-2 has prompted a worldwide pandemic and poses a great threat to public safety and global economies. Most present personal protective equipment (PPE) used to intercept pathogenic microorganisms is deficient in biocidal properties. Herein, we present green nanofibers with effective antibacterial and antiviral activities that can provide sustainable bioprotection by continuously producing reactive oxygen species (ROS). The superiority of the design is that the nanofibers can absorb and store visible light energy and maintain the activity under light or dark environment. Moreover, the nanofibers can uninterruptedly release ROS in the absence of an external hydrogen donor, acting as a biocide under all weather conditions. A facile spraying method is proposed to rapidly deploy the functional nanofibers to existing PPE, such as protective suits and masks. The modified PPE exhibit stable ROS production, excellent capacity for storing activity potential, long-term durability, and high bactericidal (>99.9%) and viricidal (>99.999%) efficacies.


Assuntos
Anti-Infecciosos/farmacologia , Hidrogênio/química , Luz , Nanofibras/química , Benzofenonas/química , Celulose/farmacologia , Nanofibras/ultraestrutura , Riboflavina/farmacologia
16.
Molecules ; 26(4)2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33670610

RESUMO

This paper presents a facile and low-cost strategy for fabrication lysozyme-loaded mesoporous silica nanotubes (MSNTs) by using silk fibroin (SF) nanofiber templates. The "top-down method" was adopted to dissolve degummed silk in CaCl2/ formic acid (FA) solvent, and the solution containing SF nanofibrils was used for electrospinning to prepare SF nanofiber templates. As SF contains a large number of -OH, -NH2 and -COOH groups, the silica layer could be easily formed on its surface by the Söber sol-gel method without adding any surfactant or coupling agent. After calcination, the MSNTs were obtained with inner diameters about 200 nm, the wall thickness ranges from 37 ± 2 nm to 66 ± 3 nm and the Brunauer-Emmett-Teller (BET) specific surface area was up to 200.48 m2/g, the pore volume was 1.109 cm3/g. By loading lysozyme, the MSNTs exhibited relatively high drug encapsulation efficiency up to 31.82% and an excellent long-term sustained release in 360 h (15 days). These results suggest that the MSNTs with the hierarchical structure of mesoporous and macroporous will be a promising carrier for applications in biomacromolecular drug delivery systems.


Assuntos
Fibroínas/química , Muramidase/metabolismo , Nanofibras/química , Nanotubos/química , Dióxido de Silício/química , Cloreto de Cálcio/química , Liberação Controlada de Fármacos , Formiatos/química , Nanofibras/ultraestrutura , Nanotubos/ultraestrutura , Porosidade , Silanos/química , Soluções , Espectrometria por Raios X , Espectroscopia de Infravermelho com Transformada de Fourier , Temperatura , Termogravimetria , Viscosidade
17.
Int J Biol Macromol ; 180: 590-598, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33711373

RESUMO

Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide. Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered. In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning. A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM). Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay. The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed. The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.


Assuntos
Quitosana/química , Miócitos Cardíacos/citologia , Nanofibras/química , Poliuretanos/química , Engenharia Tecidual/métodos , Tecidos Suporte/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Microscopia Eletrônica de Varredura/métodos , Microscopia Eletrônica de Transmissão , Nanofibras/ultraestrutura , Nanotubos de Carbono/química , Nanotubos de Carbono/ultraestrutura , Ratos , Análise Espectral Raman , Difração de Raios X
18.
Int J Biol Macromol ; 180: 510-522, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33745975

RESUMO

In this work, 2,2,6,6-tetramethylpiperidine-1-oxyl-oxidized cellulose nanocrystals (TOCNs) were loaded into sodium alginate/chitosan multilayer film as nanofillers to investigate the modulation of the surface charge density of TOCNs on the film properties. First, the surface charge density of TOCNs was controlled by adjusting the carboxyl content and morphological size by varying the oxidant dosage. After oxidation, TOCN with higher surface charge density was observed to display a higher crystallinity, a more open internal structure, a better dispersibility and a slightly weaker thermal stability. In addition, a 15-layer film composed of sodium alginate and chitosan, called (SA/CH)15, was constructed by layer-by-layer assembly. Both in situ deposition monitoring and free-standing multilayer film formation indicated that TOCNs relied on strong electrostatic interactions and hydrogen bonding to achieve a compact and uniform interlayer and a thinner thickness of (SA/CH)15, which was more evident at a high surface charge density. The addition of TOCNs also enhanced the mechanical properties, thermal stability, hydrophobicity, and barrier properties of (SA/CH)15. In particular, the resulting sodium alginate/chitosan multilayer film exhibited an improved packaging performance when nanocomposite was performed using TOCN with a surface charge density of 3.22 ± 0.11 e nm-2.


Assuntos
Alginatos/química , Celulose/química , Quitosana/química , Nanofibras/química , Nanopartículas/química , Celulose Oxidada , Interações Hidrofóbicas e Hidrofílicas , Fenômenos Mecânicos , Microscopia Eletrônica de Transmissão , Nanocompostos/química , Nanocompostos/ultraestrutura , Nanofibras/ultraestrutura , Nanopartículas/ultraestrutura , Oxirredução , Espalhamento a Baixo Ângulo , Espectroscopia de Infravermelho com Transformada de Fourier , Eletricidade Estática , Propriedades de Superfície , Difração de Raios X
19.
Int J Biol Macromol ; 181: 82-98, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-33771547

RESUMO

Vitamin D deficiency is now a global health problem; despite several drug delivery systems for carrying vitamin D due to low bioavailability and loss bioactivity. Developing a new drug delivery system to deliver vitamin D3 is a strong incentive in the current study. Hence, an implantable drug delivery system (IDDS) was developed from the electrospun cellulose acetate (CA) and ε-polycaprolactone (PCL) nanofibrous membrane, in which the core of implants consists of vitamin D3-loaded CA nanofiber (CAVD) and enclosed in a thin layer of the PCL membrane (CAVD/PCL). CA nanofibrous mat loaded with vitamin D3 at the concentrations of 6, 12, and 20% (w/w) of vitamin D3 were produced using electrospinning. The smooth and bead-free fibers with diameters ranged from 324 to 428 nm were obtained. The fiber diameters increased with an increase in vitamin D3 content. The controlled drug release profile was observed over 30-days, which fit with the zero-order model (R2 > 0.96) in the first stage. The mechanical properties of IDDS were improved. Young's modulus and tensile strength of CAVD/PCL (dry) were161 ± 14 and 13.07 ± 2.5 MPa, respectively. CA and PCL nanofibers are non-cytotoxic based on the results of the in-vitro cytotoxicity studies. This study can further broaden in-vivo study and provide a reference for developing a new IDDS to carry vitamin D3 in the future.


Assuntos
Celulose/análogos & derivados , Colecalciferol/farmacologia , Liberação Controlada de Fármacos , Nanofibras/química , Poliésteres/química , Varredura Diferencial de Calorimetria , Morte Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Celulose/química , Condutividade Elétrica , Humanos , Nanofibras/ultraestrutura , Temperatura , Viscosidade , Água/química
20.
Biomed Res Int ; 2021: 8868431, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33575351

RESUMO

Tissue engineering scaffolds with nanofibrous structures provide positive support for cell proliferation and differentiation in biomedical fields. These scaffolds are widely used for defective tissue repair and drug delivery. However, the degradation performance and mechanical properties of scaffolds are often unsatisfactory. Here, we successfully prepared a novel poly(3-hydroxybutyrate-4-hydroxybutyrate)/polypyrrole (P34HB-PPy) core-shell nanofiber structure scaffold with electrospinning and in situ surface polymerization technology. The obtained composite scaffold showed good mechanical properties, hydrophilicity, and thermal stability based on the universal material testing machine, contact angle measuring system, thermogravimetric analyzer, and other methods. The results of the in vitro bone marrow-derived mesenchymal stem cells (BMSCs) culture showed that the P34HB-PPy composite scaffold effectively mimicked the extracellular matrix (ECM) and exhibited good cell retention and proliferative capacity. More importantly, P34HB is a controllable degradable polyester material, and its degradation product 3-hydroxybutyric acid (3-HB) is an energy metabolite that can promote cell growth and proliferation. These results strongly support the application potential of P34HB-PPy composite scaffolds in biomedical fields, such as tissue engineering and soft tissue repair.


Assuntos
Hidroxibutiratos/química , Nanofibras/química , Poliésteres/química , Engenharia Tecidual/métodos , Tecidos Suporte/química , Teste de Materiais , Nanofibras/ultraestrutura , Espectroscopia de Infravermelho com Transformada de Fourier
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