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1.
Chem Commun (Camb) ; 55(67): 9955-9958, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31364619

RESUMO

A silver nanocluster-based ratiometric fluorescent nanosensor was developed for the determination of ATP in the cerebrospinal fluid of a mouse brain. Using this useful tool with good stability and high selectivity as well as a wide linear detection range, it was found that the ATP concentration in a mouse brain with Alzheimer's disease was 2300-fold higher than that in a normal one.


Assuntos
Trifosfato de Adenosina/líquido cefalorraquidiano , Química Encefálica , DNA/química , Corantes Fluorescentes/química , Nanopartículas Metálicas/química , Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Animais , Encéfalo/citologia , Encéfalo/patologia , Córtex Cerebral/química , Hipocampo/química , Camundongos , Conformação de Ácido Nucleico , Prata/química , Espectrometria de Fluorescência/métodos
2.
Chem Pharm Bull (Tokyo) ; 67(8): 733-771, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31366825

RESUMO

Transition-metal nanoparticles (NPs) catalysts supported on solid material represent one of the most important subjects in organic synthesis due to their reliable carbon-carbon or carbon-heteroatom bond-forming cross-coupling reactions. Therefore methodologically and conceptually novel immobilization methods for nonprecious transition-metal NPs are currently required for the development of organic, inorganic, green, materials, and medicinal chemistry. We discovered a self-assembled Au-supported Pd NPs catalyst (SAPd(0)) and applied it as a catalyst to Suzuki-Miyaura coupling, Buchwald-Hartwig reaction, Carbon(sp2 and sp3)-Hydrogen bond functionalization, double carbonylation, removal of the allyl protecting groups of allyl esters, and redox switching. SAPd(0) comprises approximately 10 layers of self-assembled Pd(0) NPs, whose size is less than 5 nm on the surface of a sulfur-modified Au. The Pd NPs are wrapped in a sulfated p-xylene polymer matrix. We thought that the self-assembled Au-supported Pd NPs could be made by in situ metal NP and nanospace simultaneous organization (PSSO). This methodology involves 4 kinds of simultaneous procedures: i) reduction of a higher valence metal salt, ii) growth of metal NPs with appropriate size, iii) growth of a matrix with appropriate pores, and iv) wrapping of the metal NPs by matrix nanopores. This methodology is different from previously reported metal NPs-immobilizing methods, which use solid supports with preformed pores or coordination sites. We also applied the in situ PSSO method to prepare various immobilized transition-metal NPs, including base metals. For example, the in situ PSSO method can be applicable to easily prepare Ni, Ru, and Fe NPs with good recyclability and low metal leaching for use in organic synthesis.


Assuntos
Descoberta de Drogas , Nanopartículas Metálicas/química , Compostos Orgânicos/síntese química , Elementos de Transição/química , Catálise , Estrutura Molecular , Compostos Orgânicos/química
3.
Phys Chem Chem Phys ; 21(33): 18352-18362, 2019 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-31402363

RESUMO

Graphene coated gold nanoparticles (GGNPs) have attracted great attention in recent years because of their high thermal stability and unique optical properties. In this paper, we study photothermal properties of GGNPs using the Mie and Gans theories combined with the Pennes bioheat equation. The effect of various sizes and different shapes of GGNPs such as nanosphere, nanorod and nanodisc are taken into account. The extinction efficiency and temperature distribution in tumor tissue show that graphene coated gold nanorods, because of the high temperature rise during laser irradiation, are more suitable candidates for photothermal therapy (PTT) applications. Also, we show that the extinction peak of graphene coated gold nanorods can be adjusted in the biological windows by increasing the graphene shell thickness and/or by changing their aspect ratio. Finally, we investigated the effect of the number of graphene layers upon the temperature rise in the tumor and found that the temperature rise increases with increasing number of graphene layers. Our findings introduce a new class of nanoagents which can be used in PTT applications.


Assuntos
Ouro/química , Grafite/química , Nanopartículas Metálicas/química , Nanotubos/química , Fototerapia/métodos
4.
Chem Commun (Camb) ; 55(66): 9865-9868, 2019 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-31364624

RESUMO

Au hydrogel with a well-defined nanowire network was rationally designed through one-step dopamine-induced self-assembly. Due to the porous nanostructure along with the polydopamine induced accumulation and interfacial electron transfer effects, the resulting Au hydrogel nanozyme exhibits enhanced glucose oxidase-like activity and peroxidase-like activity, with a biomimetic cascade catalysis.


Assuntos
Biomimética , Dopamina/química , Glucose Oxidase/química , Ouro/química , Hidrogéis/química , Nanopartículas Metálicas/química , Peroxidase/química , Catálise , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Oxirredução
5.
J Photochem Photobiol B ; 197: 111541, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31272033

RESUMO

Here, we report the novel fabrication of ZnO nanoparticles using the Costus igneus leaf extract. Gas chromatography-mass spectrometry (GC-MS) and proton nuclear magnetic resonance (1H NMR) spectroscopy to determine the bioactive components present in the plant extract. The synthesis of Ci-ZnO NPs (C. igneus- coated zinc oxide nanoparticles) was accomplished using a cost-effective and simple technique. Ci-ZnO NPs were specified using UV-visible spectroscopy, FTIR, XRD, and TEM. Ci-ZnO NPs was authenticated by UV-Vis and exhibited a peak at 365 nm. The XRD spectra proved the crystalline character of the Ci-ZnO NPs synthesized as hexagonal wurtzite. The FTIR spectrum illustrated the presence of possible functional groups present in Ci-ZnO NPs. The TEM micrograph showed evidence of the presence of a hexagonal organization with a size of 26.55 nm typical of Ci-ZnO NPs. The α-amylase and α-glucosidase inhibition assays demonstrated antidiabetic activity of Ci-ZnO NPs (74 % and 82 %, respectively), and the DPPH [2,2-diphenyl-1-picrylhydrazyl hydrate] assay demonstrated the antioxidant activity of the nanoparticles (75%) at a concentration of 100 µg/ml. The Ci-ZnO NPs exhibited promising antibacterial and biofilm inhibition activity against the pathogenic bacteria Streptococcus mutans, Lysinibacillus fusiformis, Proteus vulgaris, and Vibrio parahaemolyticus. Additionally, the Ci-ZnO NPs showed biocompatibility with mammalian RBCs with minimum hemolytic activity (0.633 % ±â€¯0.005 %) at a concentration of 200 µg/ml.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/química , Biofilmes/efeitos dos fármacos , Nanopartículas Metálicas/química , Extratos Vegetais/química , Óxido de Zinco/química , Antibacterianos/síntese química , Antibacterianos/química , Materiais Biocompatíveis/química , Materiais Biocompatíveis/farmacologia , Costus/química , Costus/metabolismo , Eritrócitos/citologia , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Negativas/fisiologia , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/fisiologia , Química Verde , Hemólise/efeitos dos fármacos , Humanos , Insulina/química , Nanopartículas Metálicas/toxicidade , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Extratos Vegetais/metabolismo , Folhas de Planta/química , Folhas de Planta/metabolismo , alfa-Amilases/antagonistas & inibidores , alfa-Amilases/metabolismo
6.
J Photochem Photobiol B ; 197: 111540, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31276926

RESUMO

Protein aggregation can lead to several incurable amyloidosis diseases. The full aggregation pathway is not fully understood, creating the need for new methods of studying this important biological phenomenon. Lysozyme is an amyloidogenic protein which is often used as a model protein for studying amyloidosis. This work explores the potential of employing Lysozyme encapsulated gold nanoclusters (Ly-AuNCs) to study the protein's aggregation. The fluorescence emission properties of Ly-AuNCs were studied in the presence of increasing concentrations of native lysozyme and as a function of pH, of relevance in macromolecular crowding and inflammation-triggered aggregation. AuNC fluorescence was observed to both redshift and increase in intensity as pH is increased or when native lysozyme is added to a solution of Ly-AuNCs at pH 3. The long (µs) fluorescence lifetime component of AuNC emission was observed to decrease under both conditions. Interestingly it was found via Time-Resolved Emission Spectra (TRES) that both AuNC fluorescence components increase in intensity and redshift with increasing pH while only the long lifetime component of AuNC was observed to change when adding native lysozyme to solution; indicating that the underlying mechanisms for the changes observed are fundamentally different for each case. It is possible that the sensitivity of Ly-AuNCs to native lysozyme concentration could be utilized to study early-stage aggregation.


Assuntos
Ouro/química , Nanopartículas Metálicas/química , Muramidase/química , Animais , Galinhas , Concentração de Íons de Hidrogênio , Muramidase/metabolismo , Agregados Proteicos/fisiologia , Espectrometria de Fluorescência
7.
J Cancer Res Clin Oncol ; 145(9): 2199-2209, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31309302

RESUMO

PURPOSE: Radiofrequency (RF) ablation therapy is of great interest in cancer therapy as it is non-ionizing radiation and can effectively penetrate into the tissue. However, the current RF ablation technique is invasive that requires RF probe insertion into the tissue and generates a non-specific heating. Recently, RF-responsive nanomaterials such as gold nanoparticles (AuNPs) and iron oxide nanoparticles (IONPs) have led to tremendous progress in this area. They have been found to be able to absorb the RF field and induce a localized heating within the target, thereby affording a non-invasive and tumor-specific RF ablation strategy. In the present study, for the first time, we used a hybrid core-shell nanostructure comprising IONPs as the core and AuNPs as the shell (IO@Au) for targeted RF ablation therapy. Due to the magnetic core, the nanohybrid can be directed toward the tumor through a magnet. Moreover, IONPs enable the nanohybrid to be used as a magnetic resonance imaging (MRI) contrast agent. RESULTS: In vitro cytotoxicity experiment showed that the combination of IO@Au and 13.56-MHz RF field significantly reduced the viability of cancer cells. Next, during an in vivo experiment, we demonstrated that magnetically targeting of IO@Au to the tumor and subsequent RF exposure dramatically suppressed the tumor growth. CONCLUSION: Therefore, the integration of targeting, imaging, and therapeutic performances into IO@Au nanohybrid could afford the promise to improve the effectiveness of RF ablation therapy.


Assuntos
Ablação por Cateter/métodos , Compostos Férricos/química , Ouro/química , Hipertermia Induzida/métodos , Nanopartículas de Magnetita/uso terapêutico , Neoplasias/cirurgia , Ablação por Radiofrequência/métodos , Animais , Compostos Férricos/uso terapêutico , Ouro/uso terapêutico , Imagem por Ressonância Magnética/métodos , Nanopartículas de Magnetita/química , Masculino , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Camundongos , Camundongos Endogâmicos BALB C , Terapia de Alvo Molecular/métodos , Nanocompostos/química , Nanocompostos/uso terapêutico , Nanoconchas/química , Nanoconchas/uso terapêutico , Neoplasias/patologia , Células Tumorais Cultivadas
8.
Chem Commun (Camb) ; 55(65): 9697-9700, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31347623

RESUMO

An ultra-high sensitivity sensor with the function of chiral signal amplification has been proposed for the enantiomer discrimination of various amino acid enantiomers based on charge transfer (CT)-induced SERS spectroscopy. The introduced TiO2 in this sensor improves the CT behavior and discrimination efficiency distinctly and enantiomeric discrimination is realized even at low concentration.


Assuntos
Compostos de Anilina/química , Nanopartículas Metálicas/química , Compostos de Sulfidrila/química , Triptofano/análise , beta-Ciclodextrinas/química , Ligações de Hidrogênio , Fenilalanina/análise , Fenilalanina/química , Prata/química , Análise Espectral Raman/métodos , Estereoisomerismo , Titânio/química , Triptofano/química , Tirosina/análise , Tirosina/química
10.
Chem Commun (Camb) ; 55(61): 8935-8938, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31286126

RESUMO

The Eubacterium saburreum serine protease inhibitor from the human gut microbiota inhibits the eukaryotic pancreatic elastase associated with acute pancreatitis. Interestingly, the inhibition efficiency and stability are markedly increased by the para-sulphonato-calix[8]arene capped silver nanoparticles. Moreover, this enzyme is distinguishable by its high inhibitory effect at broad pH range between 2-10 and temperatures from 10 to 40 °C, in the presence of para-sulphonato-calix[8]arene capped silver nanoparticles the enzyme remains active even at 70 °C.


Assuntos
Calixarenos/química , Nanopartículas Metálicas/química , Elastase Pancreática/antagonistas & inibidores , Serpinas/química , Prata/química , Sequência de Aminoácidos , Animais , Ensaios Enzimáticos , Eubacterium/química , Concentração de Íons de Hidrogênio , Estabilidade Proteica , Alinhamento de Sequência , Serpinas/isolamento & purificação , Ácidos Sulfônicos/química , Suínos , Temperatura Ambiente
11.
Chem Commun (Camb) ; 55(61): 9019-9022, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31290863

RESUMO

DNA nanohydrogel assembled AuNPs were proposed as a high-throughput multidimensional sensing strategy for small molecule reductant profiling in rat brain. The equilibrium among AuNPs, DNA nanohydrogel and targets produced a unique fingerprint-like pattern for differentiating the reducing capacity.


Assuntos
Encéfalo/metabolismo , DNA de Cadeia Simples/química , Ouro/química , Hidrogéis/química , Nanopartículas Metálicas/química , Substâncias Redutoras/análise , Animais , Colorimetria/métodos , Análise Discriminante , Glutationa/líquido cefalorraquidiano , Oxirredução , Tamanho da Partícula , Ratos
12.
Chem Commun (Camb) ; 55(61): 9015-9018, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31290867
13.
Chem Commun (Camb) ; 55(61): 9039-9042, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31292589
14.
J Nanobiotechnology ; 17(1): 79, 2019 Jul 05.
Artigo em Inglês | MEDLINE | ID: mdl-31277668

RESUMO

BACKGROUND: Protein nanocages have emerged as popular nanocarriers for either drug delivery or biotemplates for the preparation of nanomaterials. However, only three interfaces, namely exterior surface, intersubunit and inner cavity, have been used as reaction sites for the above purposes with all known protein nanocages. On the other hand, how to control the site of Au NCs formed within a targeted protein template while maintaining the functionality of protein itself remains challenging. RESULTS: In this work, inspired by compartmentalization in living systems, we firstly come up with the conception of "intrasubunit interfaces", located within subunit of protein nanocage. We built a new, specific compartment for fabrication of gold nanoclusters by genetic modification of the inherent ferroxidase center located within four-α-helix bundle of each ferritin subunit. This newly built compartment not only realizes the site-directed synthesis of gold nanoclusters but also has no effect on the functionality of ferritin itself such as encapsulation by its inner cavity. These redesigned composites can be further applied as fluorescent imaging agent and carriers for preparation of hybrid nanomaterials. CONCLUSIONS: The designing strategy of intrasubunit interfaces opens a new way for future applications of cage-like proteins.


Assuntos
Portadores de Fármacos/química , Ferritinas/química , Ouro/química , Nanopartículas Metálicas/química , Animais , Caenorhabditis elegans/metabolismo , Escherichia coli/genética , Ferritinas/genética , Corantes Fluorescentes/química , Células Hep G2 , Humanos , Mutação , Imagem Óptica
15.
J Agric Food Chem ; 67(28): 7886-7897, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31283218

RESUMO

Hard, medium, and soft wheat proteins, based on gluten content, were studied for their important roles in nanometallic surface chemistry. In situ synthesis of Au nanoparticles (NPs) was followed to determine the surface adsorption behavior of wheat protein based on the gluten contents. A greater amount of gluten contents facilitated the nucleation to produce Au NPs. X-ray photoelectron spectroscopy (XPS) surface analysis clearly showed the surface adsorption of protein on nanometallic surfaces which was almost equally prevalent for the hard, medium, and soft wheat proteins. Wheat protein conjugated NPs were highly susceptible to phase transfer from aqueous to organic phase that was entirely related to the amount of gluten contents. The presence of higher gluten content in hard wheat protein readily enabled the hard wheat protein conjugated NPs to move across the aqueous-organic interface followed by medium and soft wheat protein conjugated NPs. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS page) analysis allowed us to determine molar masses of nanometallic surface adsorbed protein fractions. Only two protein fractions of high molar masses (74 and 85 kDa) from SDS solubilized hard, medium, and soft wheat proteins preferred to adsorb on nanometallic surfaces out of more than 15 protein fractions of pure wheat protein. This made the surface adsorption of wheat protein highly selective and closely related to gluten content. Cetyltrimethylammonium bromide (CTAB) solubilized wheat protein conjugated NPs demonstrated their strong antimicrobial activities against both Gram negative and Gram positive bacteria making them suitable for their applications in food industry.


Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Glutens/química , Ouro/química , Nanopartículas Metálicas/química , Triticum/química , Adsorção , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Dureza
16.
J Phys Chem Lett ; 10(16): 4505-4510, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31310141

RESUMO

Owing to their excellent surface plasmonic properties, Au nanobranches have drawn increasing attention in various bioapplications, such as contrast agents for photoacoustic imaging, nanomedicines for photothermal therapy, and carriers for drug delivery. The monodispersity and plasmonic bandwidth of Au nanobranches are of great importance for the efficacy of those bioapplications. However, it is still a challenge to accurately synthesize size- and shape-controlled Au nanobranches. Here we report a facile seed-mediated growth method to synthesize monodisperse Au nanotetrapods (NTPs) with tunable and ultranarrow plasmonic bands. The NTPs have a novel D2d symmetry with four arms elongated in four ⟨110⟩ directions. The growth mechanism of NTPs relies on the delicate kinetic control of deposition and diffusion rates of adatoms. Upon laser irradiation, the PEGylated NTPs possess remarkable photothermal conversion efficiencies and photoacoustic imaging properties. The NTPs can be applied as a multifunctional theranostic agent for both photoacoustic imaging and image-guided photothermal therapy.


Assuntos
Ouro/química , Nanopartículas Metálicas/química , Nanomedicina Teranóstica , Polietilenoglicóis/química , Temperatura Ambiente
17.
J Photochem Photobiol B ; 197: 111539, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31301638

RESUMO

Treatment of burn injury is clinically challenging one, therefore several steps and noteworthy approaches have been taken to improve wound mechanisms. Citrus pectin plays a stabilizing agent to synthesis of ZnO nanoparticles (ZnO NPs). The present study is focused on ZnO loaded collagen/chitosan nanofibrous were synthesized by electrospinning method using ZnO NPs. The chemical structure, phase purity and morphological observation were investigated under spectroscopic and mircoscopic techniques and demonstrated their suitable properties as a wound healing material. In addition, that prepared nanoparticles loaded biopolymeric fibrous nanomaterial showed suitable antibacterial activity against S. aureus and E. coli bacterial pathogens and also in vitro studies was confirmed the enhanced proliferation, cell viability and biocompatibility. In vitro evaluations have been exhibited acceptable cell proliferation is observed throughout the ZnO loaded Coll/CS nanofibrous within 3 days, which was comparable to the control material. In vivo wound healing ability was monitored on the rat wound experimental model. From the in vivo observations, revealed that the loaded of ZnO NPs with Coll/CS nanofibrous can effectively quicken wound healing mechanism, expressed in the initial stage healing process. These results suggest that ZnO loaded collagen/chitosan nanofibrous is a potential candidate for wound healing applications with enhanced biological properties.


Assuntos
Queimaduras/patologia , Quitosana/química , Colágeno/química , Nanopartículas Metálicas/química , Nanofibras/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Bacteriemia/prevenção & controle , Queimaduras/veterinária , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Humanos , Nanofibras/uso terapêutico , Nanofibras/toxicidade , Ratos , Pele/efeitos dos fármacos , Pele/patologia , Staphylococcus aureus/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Óxido de Zinco/química
18.
J Photochem Photobiol B ; 197: 111556, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31326842

RESUMO

Facile green synthesis of copper nanoparticles from different biological procedures has been indicated, but among all, biosynthesis of copper nanoparticles from medicinal plants is considered as the most suitable method. The use of medicinal plant material increases the therapeutical effects of copper nanoparticles. The aim of this study was green synthesis of copper nanoparticles from aqueous extract of Falcaria vulgaris leaf (CuNPs) and assessment of their cytotoxicity, antioxidant, antifungal, antibacterial, and cutaneous wound healing properties. These nanoparticles were characterized by X-ray diffraction (XRD), fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV), transmission electron microscopy (TEM), and field emission scanning electron microscopy (FE-SEM) analysis. The synthesized CuNPs had great cell viability dose-dependently (Investigating the effect of the CuNPs on human umbilical vein endothelial cell (HUVEC) line) and indicated this method was nontoxic. Also, 2,2-diphenyl-1-picrylhydrazyl (DPPH) test was done to assess the antioxidant activities, which indicated similar antioxidant potentials for CuNPs and butylated hydroxytoluene. In part of cutaneous wound healing property of CuNPs, after creating the cutaneous wound, the rats were randomly divided into six groups: treatment with 0.2% CuNPs ointment, treatment with 0.2% CuSO4 ointment, treatment with 0.2% F. vulgaris ointment, treatment with 3% tetracycline ointment, treatment with Eucerin basal ointment, and untreated control. These groups were treated for 10 days. Treatment with CuNPs ointment remarkably increased (p ≤ .01) the wound contracture, vessel, hexosamine, hydroxyl proline, hexuronic acid, fibrocyte, and fibrocytes/fibroblast rate and substantially reduced (p ≤ .01) the wound area, total cells, neutrophil, and lymphocyte compared to other groups. In antibacterial and antifungal parts of this research, the concentration of CuNPs with minimum dilution and no turbidity was considered minimum inhibitory concentration (MIC). To determine minimum fungicidal concentration (MFC) and minimum bactericidal concentration (MBC), 60 µL MIC and three preceding chambers were cultured on Sabouraud Dextrose Agar and Muller Hinton Agar, respectively. The minimum concentration with no fungal and bacterial growth were considered MFC and MBC, respectively. CuNPs inhibited the growth of all fungi at 2-4 mg/mL concentrations and removed them at 4-8 mg/mL concentrations (p ≤ .01). In case of antibacterial effects of CuNPs, they inhibited the growth of all bacteria at 2-8 mg/mL concentrations and removed them at 4-16 mg/mL concentrations (p ≤ .01). The results of XRD, FT-IR, UV, TEM, and FE-SEM confirm that the aqueous extract of F. vulgaris leaf can be used to yield copper nanoparticles with notable amount of antioxidant, antifungal, antibacterial, and cutaneous wound healing potentials without any cytotoxicity. Further clinical trials are necessary for confirmation these therapeutical effects of CuNPs in human.


Assuntos
Apiaceae/química , Cobre/química , Nanopartículas Metálicas/química , Extratos Vegetais/química , Animais , Antibacterianos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Antifúngicos/síntese química , Antifúngicos/química , Antifúngicos/farmacologia , Antioxidantes/química , Apiaceae/metabolismo , Candida/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Química Verde , Células Endoteliais da Veia Umbilical Humana , Humanos , Masculino , Nanopartículas Metálicas/toxicidade , Testes de Sensibilidade Microbiana , Folhas de Planta/química , Folhas de Planta/metabolismo , Ratos , Pele/efeitos dos fármacos , Pele/patologia , Cicatrização/efeitos dos fármacos
19.
J Photochem Photobiol B ; 197: 111536, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31326846

RESUMO

The latent utilization of biomaterials that are osteo-conducive in the advancement of healing bone fracture has fascinated extensive consideration. This work includes the synthesis of silver nanoparticles (AgNPs) with the help of a Bauhinia acuminate plant flower extract through an ecofriendly synthetic process without any use of harmful reductants. In the fabrication of AgNPs, Bauhinia acuminate plant flower extract bio constituents acts as both stabilizing and reducing agent. The studies of Fourier transform infrared (FTIR) and X-ray diffraction (XRD) techniques confirmed the formation of AgNPS. TEM images revealed that AgNPs are uniform with average particle size of 17 nm. Further, this work explored if silver nanoparticles (AgNPs) might endorse the osteogenesis and proliferation of mesenchymal stem cells (MSCs) and advance the curing of bone fractures. We also exhibited that the prepared AgNPs could promote the in -vitro osteogenic differentiation and proliferation of MSCs'. Also, the prepared AgNps could stimulate the proliferation of mMSCs at specific concentrations of 6-20 µM. Further, cell viability studies showed that AgNPs exhibited no reduction in mouse mesenchymal stem cell viability at <4 µM. Further, these results indicated the induction effects of AgNPs on osteogenic differentiation and proliferation on MSCs, as well as the advancement of meniscus injury healing.


Assuntos
Bauhinia/química , Nanopartículas Metálicas/química , Osteogênese , Extratos Vegetais/química , Prata/química , Animais , Bauhinia/metabolismo , Diferenciação Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Flores/química , Flores/metabolismo , Consolidação da Fratura/efeitos dos fármacos , Química Verde , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/metabolismo , Nanopartículas Metálicas/toxicidade , Camundongos , Microscopia Eletrônica de Transmissão , Osteogênese/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier
20.
Anal Bioanal Chem ; 411(21): 5437-5444, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31300858

RESUMO

To meet the need for high-throughput immunoassays, many multiplex fluorescent immunoassays have been proposed. Most of them need different kinds of fluorescent label indicators during the test. In this work, a novel indirect ELISA-inspired dual-channel fluorescent immunoassay based on 3-mercaptopropionic acid capped CdTe/ZnS quantum dots (QDs) was constructed. The ELISA wells were coated with two kinds of antigen-QD complex. When the primary antibodies were present in a sample, they mediated the binding of a secondary antibody-DNA-gold nanoparticle complex to the antigen-QD complex. Then the gold nanoparticles quenched the fluorescence of the QDs and a decrease in fluorescence intensity was observed. Thus, the amount of primary antibody could be estimated from the decrease of fluorescence intensity. Owing to the wide absorption range and the relatively narrow emission band of the QDs, the dual-channel fluorescent immunoassay system could work at the same excitation wavelength and the emission wavelengths of each channel had no interference. As a result, two different kinds of primary antibody could be detected at the same time in one ELISA well, which simplified the operation and greatly improved the efficiency. Besides, only one type of secondary antibody needs to be added to the prepared microtiter plates, which further simplified the operation during the detection procedure. This dual-channel fluorescent immunoassay system will provide new insights into high-throughput immunodetection. Graphical abstract.


Assuntos
Compostos de Cádmio/química , Ensaio de Imunoadsorção Enzimática/métodos , Imunofluorescência/métodos , Pontos Quânticos , Sulfetos/química , Telúrio/química , Compostos de Zinco/química , Nanopartículas Metálicas/química , Microscopia Eletrônica de Transmissão
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