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1.
Nat Commun ; 10(1): 2839, 2019 06 28.
Artigo em Inglês | MEDLINE | ID: mdl-31253798

RESUMO

Immunomodulatory therapies are becoming a paradigm-shifting treatment modality for cancer. Despite promising clinical results, cancer immunotherapy is accompanied with off-tumor toxicity and autoimmune adverse effects. Thus, the development of smarter systems to regulate immune responses with superior spatiotemporal precision and enhanced safety is urgently needed. Here we report an activatable engineered immunodevice that enables remote control over the antitumor immunity in vitro and in vivo with near-infrared (NIR) light. The immunodevice is composed of a rationally designed UV light-activatable immunostimulatory agent and upconversion nanoparticle, which acts as a transducer to shift the light sensitivity of the device to the NIR window. The controlled immune regulation allows the generation of effective immune response within tumor without disturbing immunity elsewhere in the body, thereby maintaining the antitumor efficacy while mitigating systemic toxicity. The present work illustrates the potential of the remote-controlled immunodevice for triggering of immunoactivity at the right time and site.


Assuntos
Raios Infravermelhos , Nanopartículas/efeitos da radiação , Fototerapia/métodos , Animais , Linhagem Celular Tumoral , Feminino , Imunomodulação , Macrófagos , Neoplasias Mamárias Experimentais/terapia , Camundongos , Camundongos Endogâmicos BALB C , Células RAW 264.7 , Raios Ultravioleta
2.
Chem Commun (Camb) ; 55(46): 6523-6526, 2019 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-31099806

RESUMO

Cu2-xSe nanoparticles (Cu2-xSeNPs) were camouflaged with a red blood cell membrane (RBC) to create nanoparticles with improved biocompatibility, longer blood retention times, excellent absorption properties, superior photothermal conversion efficiency (67.2%) and singlet oxygen production capabilities for the synergistic photothermal and photodynamic therapy of cancer in the second near-infrared (NIR-II) window.


Assuntos
Antineoplásicos/uso terapêutico , Membrana Eritrocítica/química , Nanopartículas/uso terapêutico , Neoplasias/tratamento farmacológico , Animais , Antineoplásicos/síntese química , Antineoplásicos/efeitos da radiação , Antineoplásicos/toxicidade , Linhagem Celular Tumoral , Cobre/química , Temperatura Alta , Humanos , Hipertermia Induzida/métodos , Raios Infravermelhos , Camundongos , Nanopartículas/química , Nanopartículas/efeitos da radiação , Nanopartículas/toxicidade , Fotoquimioterapia/métodos , Células RAW 264.7 , Selênio/química , Oxigênio Singlete/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
3.
Talanta ; 201: 126-133, 2019 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-31122402

RESUMO

Upconversion nanoparticle-based lateral flow assays (UCNP-LFAs) have attracted significant attention in point-of-care testing (POCT) applications, due to the long-term photostability and enhanced signal-to-background noise ratio. The existing UCNP-LFAs generally require peripheral equipment for exciting fluorescent signals and reading out fluorescence results, which are generally bulky and expensive. Herein, we developed a miniaturized and portable UCNP-LFA platform, which is composed of a LFA detection system, an UCNP-LFA reader and a smartphone-assisted UCNP-LFA analyzer. The LFA detection system is based on three types of UCNPs for multiplexed detection. The reader has a dimension of 24.0 cm × 9.4 cm × 5.4 cm (L × W × H) and weight of 0.9 kg. The analyzer based on the custom-designed software of a smartphone (termed as UCNP-LFA analyzer) can get the quantitative analysis results in a real-time manner. We demonstrated the universality of this platform by highly sensitive and quantitative detections of several kinds of targets, including small molecule (ochratoxin A, OTA), heavy metal ion (Hg2+), bacteria (salmonella, SE), nucleic acid (hepatitis B virus, HBV) and protein (growth stimulation expressed gene 2, ST-2). Our developed UCNP-LFA platform holds great promise for applications in disease diagnostics, environmental pollution monitoring and food safety at the point of care.


Assuntos
Imunoensaio/métodos , Nanopartículas/química , Testes Imediatos , Anticorpos/química , Biomarcadores/sangue , DNA/análise , DNA/química , DNA/genética , Érbio/química , Fluoretos/química , Fluoretos/efeitos da radiação , Vírus da Hepatite B/genética , Humanos , Imunoensaio/instrumentação , Proteína 1 Semelhante a Receptor de Interleucina-1/sangue , Proteína 1 Semelhante a Receptor de Interleucina-1/imunologia , Limite de Detecção , Mercúrio/análise , Nanopartículas/efeitos da radiação , Hibridização de Ácido Nucleico , Salmonella/isolamento & purificação , Smartphone , Espectrometria de Fluorescência/métodos , Itérbio/química , Ítrio/química , Ítrio/efeitos da radiação
4.
Nano Lett ; 19(4): 2614-2619, 2019 04 10.
Artigo em Inglês | MEDLINE | ID: mdl-30848602

RESUMO

Design and engineering of highly efficient light-harvesting nanomaterial systems to emulate natural photosynthesis for maximizing energy conversion have stimulated extensive efforts. Here we present a new class of photoactive semiconductor nanocrystals that exhibit high-efficiency energy transfer for enhanced photocatalytic hydrogen production under visible light. These nanocrystals are formed through noncovalent self-assembly of In(III) meso-tetraphenylporphine chloride (InTPP) during microemulsion assisted nucleation and growth process. Through kinetic control, a series of uniform nanorods with controlled aspect ratio and high crystallinity have been fabricated. Self-assembly of InTPP porphyrins results in extensive optical coupling and broader coverage of the visible spectrum for efficient light harvesting. As a result, these nanocrystals display excellent photocatalytic hydrogen production and photostability under the visible light in comparison with the commercial InTPP porphyrin powders.


Assuntos
Catálise , Hidrogênio/química , Nanopartículas/química , Porfirinas/química , Emulsões/efeitos da radiação , Transferência de Energia/efeitos da radiação , Luz , Nanopartículas/efeitos da radiação , Nanoestruturas/química , Fotossíntese/efeitos da radiação , Porfirinas/síntese química
5.
Carbohydr Polym ; 214: 221-233, 2019 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-30925992

RESUMO

This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging. The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell. The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm. In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy. DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release. In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser. Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.


Assuntos
Antineoplásicos/farmacologia , Meios de Contraste/química , Docetaxel/farmacologia , Portadores de Fármacos/química , Ácido Hialurônico/química , Nanopartículas/química , Animais , Antineoplásicos/administração & dosagem , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Meios de Contraste/administração & dosagem , Meios de Contraste/efeitos da radiação , Meios de Contraste/toxicidade , Docetaxel/administração & dosagem , Portadores de Fármacos/administração & dosagem , Portadores de Fármacos/efeitos da radiação , Portadores de Fármacos/toxicidade , Liberação Controlada de Fármacos , Feminino , Corantes Fluorescentes/química , Humanos , Ácido Hialurônico/administração & dosagem , Ácido Hialurônico/efeitos da radiação , Ácido Hialurônico/toxicidade , Raios Infravermelhos , Injeções Intravenosas , Pulmão/patologia , Neoplasias Pulmonares/prevenção & controle , Neoplasias Pulmonares/secundário , Camundongos Endogâmicos BALB C , Nanopartículas/administração & dosagem , Nanopartículas/efeitos da radiação , Nanopartículas/toxicidade , Tamanho da Partícula , Fosfolipídeos/administração & dosagem , Fosfolipídeos/química , Fosfolipídeos/efeitos da radiação , Fosfolipídeos/toxicidade , Técnicas Fotoacústicas/métodos , Polímeros/administração & dosagem , Polímeros/química , Polímeros/efeitos da radiação , Polímeros/toxicidade , Pirróis/administração & dosagem , Pirróis/química , Pirróis/efeitos da radiação , Pirróis/toxicidade , Temperatura Ambiente , Nanomedicina Teranóstica/métodos
6.
Nat Commun ; 10(1): 1192, 2019 03 13.
Artigo em Inglês | MEDLINE | ID: mdl-30867429

RESUMO

Development of photothermal materials which are able to harness sunlight and convert it to thermal energy seems attractive. Besides carbon-based nanomaterials, conjugated polymers are emerging promising photothermal materials but their facile syntheses remain challenging. In this work, by modification of a CBT-Cys click condensation reaction and rational design of the starting materials, we facilely synthesize conjugated polymers poly-2-phenyl-benzobisthiazole (PPBBT) and its dihexyl derivative with good photothermal properties. Under the irradiation of either sunlight-mimicking Xe light or near-infrared laser, we verify that PPBBT has comparable photothermal heating-up speed to that of star material single-wall carbon nanotube. Moreover, PPBBT is used to fabricate water-soluble NPPPBBT nanoparticles which maintain excellent photothermal properties in vitro and photothermal therapy effect on the tumours exposed to laser irradiation. We envision that our synthetic method provides a facile approach to fabricate conjugated polymers for more promising applications in biomedicine or photovoltaics in the near future.


Assuntos
Hipertermia Induzida/métodos , Nanopartículas/efeitos da radiação , Neoplasias/terapia , Nanomedicina Teranóstica/métodos , Terapia Ultravioleta/métodos , Animais , Linhagem Celular Tumoral/transplante , Terapia Combinada/métodos , Modelos Animais de Doenças , Feminino , Humanos , Hipertermia Induzida/instrumentação , Lasers , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica de Transmissão , Nanopartículas/administração & dosagem , Nanopartículas/química , Nanopartículas/ultraestrutura , Polímeros/administração & dosagem , Polímeros/química , Polímeros/farmacocinética , Polímeros/efeitos da radiação , Distribuição Tecidual , Resultado do Tratamento , Terapia Ultravioleta/instrumentação
7.
Methods Appl Fluoresc ; 7(2): 022002, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30822759

RESUMO

The right choice of a fluorescent probe is essential for successful luminescence imaging and sensing and especially concerning in vivo and in vitro applications, the development of new classes have gained more and more attention in the last years. One of the most promising class are upconversion nanoparticles (UCNPs)-inorganic nanocrystals capable to convert near-infrared light in high energy radiation. In this review we will compare UCNPs with other fluorescent probes in terms of (a) the optical properties of the probes, such as their brightness, photostability and excitation wavelength; (b) their chemical properties such as the dispersibility, stability under experimental or physiological conditions, availability of chemical modification strategies for labelling; and (c) the potential toxicity and biocompatibility of the probe. Thereby we want to provide a better understanding of the advantages and drawbacks of UCNPs and address future challenges in the design of the nanocrystals.


Assuntos
Carbono/química , Corantes Fluorescentes/química , Proteínas de Fluorescência Verde/química , Elementos da Série dos Lantanídeos/química , Nanopartículas/química , Pontos Quânticos/química , Animais , Linhagem Celular Tumoral , Fluorescência , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/efeitos da radiação , Proteínas de Fluorescência Verde/síntese química , Proteínas de Fluorescência Verde/efeitos da radiação , Humanos , Elementos da Série dos Lantanídeos/efeitos da radiação , Luz , Nanopartículas/efeitos da radiação , Imagem Óptica , Pontos Quânticos/efeitos da radiação
8.
Environ Monit Assess ; 191(4): 206, 2019 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-30838462

RESUMO

Titania nanoparticles with two crystalline structures (anatase-rutile) and coated titania with different amount of tin dioxide (A-R-S#1 and A-R-S#2) are prepared through hydrolysis method. X-ray diffraction analysis (XRD) confirmed the presence of both anatase and rutile structures in the synthesized titania nanoparticles. In addition, the characteristic peak of tin dioxide in A-R-S#2 was higher than that of A-R-S#1. The photocatalytic activity of the prepared photocatalysts was evaluated using a vapor mercury lamp (150 W) for decomposition of methylene blue. The results of the photocatalytic activity variation showed that the photocatalytic activity of all the synthesized photocatalysts increases by increasing the UV irradiation time (in the range of 5 min to 20 min) and weight fraction of photocatalysts (pH = 4, pH = 8, pH = 12). The pH of suspension had negative effect on photocatalytic activity. The least and most photocatalytic activity belonged to the synthesized anatase-rutile titania and A-R-S#2, respectively. Increasing the amount of tin dioxide nanoparticles on the surface of titania leads to the enhancement of photocatalytic activity. The results of the analysis of variance showed that all the main factors and their binary and triple interactions significantly affect photocatalytic activity.


Assuntos
Azul de Metileno/química , Nanopartículas , Compostos de Estanho , Titânio , Poluentes Químicos da Água/química , Catálise , Nanopartículas/química , Nanopartículas/efeitos da radiação , Fotólise , Compostos de Estanho/química , Compostos de Estanho/efeitos da radiação , Titânio/química , Titânio/efeitos da radiação , Raios Ultravioleta
9.
J Environ Sci (China) ; 79: 213-224, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30784444

RESUMO

In this study, a baffled photocatalytic reactor was used for the treatment of colored wastewater containing the azo dye of Acid Orange 52 (AO52). A study on the active species of the photocatalytic process using TiO2 nanoparticles indicated that hydroxyl radical and superoxide have the greatest contribution to the dye degradation process respectively. Given that a level of biological oxygen demand/chemical oxygen demand (BOD5/COD) equal to 0.4 was achieved after about 5 hr from the beginning of the experiment, the reactor seems to be capable of purifying the wastewater containing AO52 dye after this time in order to discharge into a biological treatment system to continue the treatment process. The results of the liquid chromatography-mass spectrometry (LC-MS) test showed that during the first 4 hr of the experiment, with the breakdown of the azo bond, the contaminant was decomposed into the benzene annular compounds with less toxicity indicating a reduction in the toxicity of wastewater after removing the dye agent. The study on the kinetics of these reactions followed the pseudo-first-order kinetic model in all conditions and corresponded well to Langmuir-Hinshelwood model. According to the kinetic model for the simultaneous occurrence of possible pathways, the kinetic constant of production and degradation of intermediate products in optimal conditions was estimated to be between 0.0029 and 0.0391 min-1.


Assuntos
Compostos Azo/química , Corantes/química , Nanopartículas/efeitos da radiação , Titânio/efeitos da radiação , Poluentes Químicos da Água/química , Catálise , Cinética , Luz , Nanopartículas/química , Fotólise , Titânio/química , Eliminação de Resíduos Líquidos/métodos
10.
Nat Commun ; 10(1): 406, 2019 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-30679425

RESUMO

Infrared-light-induced carrier transfer is a key technology for 'invisible' optical devices for information communication systems and energy devices. However, clear and colourless photo-induced carrier transfer has not yet been demonstrated in the field of photochemistry, to the best of our knowledge. Here, we resolve this problem by employing short-wavelength-infrared (1400-4000 nm) localized surface plasmon resonance-induced electron injection from indium tin oxide nanocrystals to transparent metal oxides. The time-resolved infrared measurements visualize the dynamics of the carrier in this invisible system. Selective excitation of localized surface plasmon resonances causes hot electron injection with high efficiency (33%) and long-lived charge separation (~ 2-200 µs). We anticipate our study not only provides a breakthrough for plasmonic carrier transfer systems but may also stimulate the invention of state-of-the-art invisible optical devices.


Assuntos
Raios Infravermelhos , Nanopartículas/química , Nanopartículas/efeitos da radiação , Fotoquímica , Ressonância de Plasmônio de Superfície , Compostos de Estanho/química , Compostos de Estanho/efeitos da radiação , Cinética , Microscopia Eletrônica de Transmissão , Tamanho da Partícula , Fenômenos Físicos , Dióxido de Silício/química , Espectroscopia de Luz Próxima ao Infravermelho , Propriedades de Superfície
11.
Clin Biochem ; 66: 76-82, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30695682

RESUMO

OBJECTIVES: New user-friendly diagnostic tests for detection of individuals infected by Mycobacterium leprae (M. leprae), the causative pathogen of leprosy, can help guide therapeutic and prophylactic treatment, thus positively contributing to clinical outcome and reduction of transmission. To facilitate point-of-care testing without the presence of phlebotomists, the use of fingerstick blood (FSB) rather than whole blood-derived serum is preferred. This study is a first proof-of-principle validating that previously described rapid serum tests detecting antibodies and cytokines can also be used with FSB. METHODS: Quantitative detection of previously identified biomarkers for leprosy and M. leprae infection, anti-M. leprae PGL-I IgM antibodies (αPGL-I), IP-10 and CRP, was performed with lateral flow (LF) strips utilizing luminescent up-converting reporter particles (UCP) and a portable reader generating unbiased read-outs. Precise amounts of FSB samples were collected using disposable heparinized capillaries. Biomarker levels in paired FSB and serum samples were determined using UCP-LF test strips for leprosy patients and controls in Bangladesh, Brazil, South-Africa and the Netherlands. RESULTS: Correlations between serum and FSB from the same individuals for αPGL-I, CRP and IP-10 were highly significant (p < .0001) even after FSB samples had been frozen. The αPGL-I FSB test was able to correctly identify all multibacillary leprosy patients presenting a good quantitative correlation with the bacterial index. CONCLUSIONS: Reader-assisted, quantitative UCP-LF tests for the detection of humoral and cellular biomarkers for M. leprae infection, are compatible with FSB. This allows near-patient testing for M. leprae infection and immunomonitoring of treatment without highly trained staff. On site availability of test-result concedes immediate initiation of appropriate counselling and treatment. Alternatively, the UCP-LF format allows frozen storage of FSB samples compatible with deferred testing in central laboratories.


Assuntos
Anticorpos/sangue , Análise Química do Sangue/métodos , Proteína C-Reativa/análise , Quimiocina CXCL10/sangue , Hanseníase/diagnóstico , Resinas Acrílicas/química , Animais , Anticorpos/imunologia , Antígenos de Bactérias/imunologia , Biomarcadores/sangue , Análise Química do Sangue/instrumentação , Feminino , Cabras , Humanos , Raios Infravermelhos , Masculino , Camundongos , Mycobacterium leprae/imunologia , Nanopartículas/química , Nanopartículas/efeitos da radiação , Testes Imediatos
12.
Int J Pharm ; 557: 66-73, 2019 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-30580088

RESUMO

Graphene oxide (GO) owns huge surface area and high drug loading capacity for aromatic molecules, such as doxorubicin (DOX). However, its biocompatibility is poor and it might agglomerate in physiological conditions. Chemical modification of GO with hydrophilicpolymer, especially PEGylation, was a common method to improve its biocompatibility. But the chemical modification of GO was complicated, and its drug loading capacity might be reduced because of the occupation of its functional groups. In this study, DOX-PEG polymers with different PEG molecular weights were synthesized to modify nano graphene oxide (NGO) to simultaneously realize the solubilization of NGO and the high loading capacity of DOX. The result showed that the drug release of NGO@DOX-PEG was pH sensitive. NIR irradiation could augment the drug release, cellular uptake, cytotoxicity and nuclear translocation of nanodrugs. Among the three kinds of nanodrugs, NGO@DOX-PEG5K was superior to others. It suggested that after conjugating with PEG, the bond between DOX-PEG and NGO was weakened, which resulted in a better drug release and treatment effect. In summary, the NIR and pH dual-responsive NGO@DOX-PEG nanodrugs were developed by noncovalent modification, and it demonstrated excellent biocompatibility and photochemical therapeutic effect, presenting a promising candidate for antitumor therapy, especially NGO@DOX-PEG5K.


Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Doxorrubicina/administração & dosagem , Portadores de Fármacos/administração & dosagem , Grafite/administração & dosagem , Nanopartículas/administração & dosagem , Óxidos/administração & dosagem , Polietilenoglicóis/administração & dosagem , Antibióticos Antineoplásicos/química , Antibióticos Antineoplásicos/efeitos da radiação , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos da radiação , Doxorrubicina/química , Doxorrubicina/efeitos da radiação , Portadores de Fármacos/química , Portadores de Fármacos/efeitos da radiação , Liberação Controlada de Fármacos , Grafite/química , Grafite/efeitos da radiação , Células HeLa , Humanos , Luz , Nanopartículas/química , Nanopartículas/efeitos da radiação , Óxidos/química , Óxidos/efeitos da radiação , Fotoquimioterapia , Polietilenoglicóis/química , Polietilenoglicóis/efeitos da radiação , Espécies Reativas de Oxigênio/metabolismo , Solubilidade
13.
Int J Pharm ; 557: 182-191, 2019 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-30584908

RESUMO

Nanoparticles based on biodegradable polymers are well-known as approved carrier systems for a diversity of drugs. Despite their advantages, such as the option of an active drug targeting or the physicochemical protection of instable payloads, the controlled drug release often underlies intra- and interindividual influences and is therefore difficult to predict. To circumvent this limitation, the release behavior can be optimized using light-responsive materials for the nanoparticle preparation. The resulting light-responsive nanoparticles are able to release the embedded drug after an external light-stimulus, thereby increasing efficacy and safety of the therapy. In the present study light-responsive self-immolative polymers were used for the nanoparticle manufacturing. Light-responsive polycarbonates (LrPC) as well as PEGylated LrPC (LrPC-PEG) were synthesized via ring-opening polymerization of trimethylene carbonate-based monomers and fully physico-chemically characterized. Light-responsive nano formulations were obtained by blending LrPC or (LrPC-PEG) with the FDA-approved polymer poly(DL-lactide-co-glycolide) (PLGA). The nanoparticles were loaded with the photosensitizer 5,10,15,20-tetrakis(m-hydroxyphenyl)chlorin (mTHPC). The light-induced nanoparticle degradation was analyzed as well as the drug release behavior with and without illumination. Furthermore, biological safety of the degradation products was investigated in an in vitro cell culture study.


Assuntos
Sistemas de Liberação de Medicamentos , Luz , Mesoporfirinas/administração & dosagem , Nanopartículas , Fármacos Fotossensibilizantes/administração & dosagem , Polímeros , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Humanos , Nanopartículas/administração & dosagem , Nanopartículas/efeitos da radiação , Fotoquimioterapia , Polímeros/administração & dosagem , Polímeros/efeitos da radiação
14.
Nanoscale ; 10(38): 18124-18130, 2018 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-30255925

RESUMO

Photodynamic therapy (PDT) is a non-invasive phototherapy that has gained significant attention for cancer therapy. However, image-guided PDT still remains a considerable challenge. Herein, we developed a targeted, near-infrared (NIR) fluorescence imaging nanoprobe for cancer cells by preparing natural biomass carbon dots (NBCDs) from lychee exocarp, and loading transferrin and a photosensitizer on the NBCD surfaces for image-guided PDT of cancer cells and mouse tumors. Because the surfaces of cancer cells exhibit more transferrin receptors, the proposed NIR fluorescent nanoprobe can better penetrate cancer cells for cancer cell targetable fluorescence imaging. Thus, the dual-function nanoprobe made from natural biomass can be used as a specific agent for NIR fluorescence imaging and PDT. More importantly, the functional nanoprobe prepared from natural biomass emits NIR fluorescence, shows very low biological toxicity, and can minimize side effects on normal cells. After directly injecting the nanoprobes into tumor tissues, the photosensitizers on the surface of the NBCDs can produce singlet oxygen (1O2) through photodynamic reactions when irradiated with 650 nm light to kill cancer cells, thus inhibiting tumor growth in PDT-treated mice. Therefore, the functional fluorescent nanoprobe made from natural biomass has been employed as a PDT agent, and holds great promise in image-guided tumor PDT.


Assuntos
Biomassa , Carbono/química , Litchi/química , Nanopartículas/administração & dosagem , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fotoquimioterapia/métodos , Animais , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Feminino , Fluorescência , Humanos , Raios Infravermelhos , Camundongos , Nanopartículas/química , Nanopartículas/efeitos da radiação , Nanopartículas/uso terapêutico , Fármacos Fotossensibilizantes/administração & dosagem , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/farmacologia , Oxigênio Singlete/análise , Nanomedicina Teranóstica , Transferrina/administração & dosagem , Transferrina/química
15.
Inorg Chem ; 57(16): 10137-10145, 2018 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-30074794

RESUMO

We report herein a new ZIF-90-based PDT agent which was synthesized by in situ assembly of imidazole-2-carboxaldehyde (IcaH), Zn(NO3)2, and heavy atom iodine-attached Bodipy. The obtained 2I-BodipyPhNO2@ZIF-90 (1) host-guest photosensitive system featured low cytotoxicity, good biocompatibility, pH-driven selective cancer cell uptake and release, mitochondria targeting, and highly efficient pH-triggered 1O2 generation. Therefore, it can be used as a high-performing PDT agent to selectively kill tumor cells. In comparison to free 2I-BodipyPhNO2, 1 exhibits a much higher antitumor efficacy and selectivity, which was confirmed by in vitro cell experiments.


Assuntos
Antineoplásicos/farmacologia , Compostos de Boro/farmacologia , Estruturas Metalorgânicas/farmacologia , Nanopartículas/química , Fármacos Fotossensibilizantes/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/efeitos da radiação , Antineoplásicos/toxicidade , Apoptose/efeitos dos fármacos , Compostos de Boro/síntese química , Compostos de Boro/efeitos da radiação , Compostos de Boro/toxicidade , Linhagem Celular Tumoral , Estabilidade de Medicamentos , Humanos , Concentração de Íons de Hidrogênio , Estruturas Metalorgânicas/síntese química , Estruturas Metalorgânicas/efeitos da radiação , Estruturas Metalorgânicas/toxicidade , Mitocôndrias/metabolismo , Nanopartículas/efeitos da radiação , Nanopartículas/toxicidade , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/efeitos da radiação , Fármacos Fotossensibilizantes/toxicidade , Oxigênio Singlete/metabolismo
16.
Langmuir ; 34(34): 9974-9981, 2018 08 28.
Artigo em Inglês | MEDLINE | ID: mdl-30056720

RESUMO

A pH- and ultrasound dual-responsive drug release pattern was successfully achieved using mesoporous silica nanoparticles (MSNs) coated with polydopamine (PDA). In this paper, the PDA shell on the MSN surface was obtained through oxidative self-polymerization under the alkaline condition. The morphology and structure of this composite nanoparticle were fully characterized by a series of analyses, such as infrared (IR), transmission electron microscopy, and thermogravimetric analysis. Doxorubicin hydrochloride (DOX)-loaded composite nanoparticles were used to study the performances of responsive drug storage/release behavior, and this kind of hybrid material displayed an apparent pH response in DOX releasing under the acidic condition. Beyond that, upon high-intensity focused ultrasound exposure, loaded DOX in composite nanoparticles was successfully triggered to release from pores because of the ultrasonic cavitation effect, and the DOX-releasing pattern could be optimized into a unique pulsatile fashion by switching the on/off status. From the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, it was observed that our blank nanoparticles showed no toxicity to HeLa cells, but DOX-loaded nanoparticles could inhibit the growth of tumor cells. Furthermore, these composite nanoparticles displayed an effective near-IR photothermal conversion capability with a relatively high conversion efficiency (∼37%). These as-desired drug delivery carriers might have a great potential for future cancer treatment that combine the chemotherapy and photothermal therapy.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Doxorrubicina/farmacologia , Portadores de Fármacos/química , Indóis/química , Nanopartículas/química , Polímeros/química , Dióxido de Silício/química , Antibióticos Antineoplásicos/química , Antibióticos Antineoplásicos/toxicidade , Doxorrubicina/química , Doxorrubicina/toxicidade , Portadores de Fármacos/efeitos da radiação , Portadores de Fármacos/toxicidade , Liberação Controlada de Fármacos , Células HeLa , Humanos , Ligações de Hidrogênio , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Indóis/síntese química , Indóis/efeitos da radiação , Indóis/toxicidade , Raios Infravermelhos , Nanocompostos/química , Nanocompostos/efeitos da radiação , Nanocompostos/toxicidade , Nanopartículas/efeitos da radiação , Nanopartículas/toxicidade , Polímeros/síntese química , Polímeros/efeitos da radiação , Polímeros/toxicidade , Porosidade , Dióxido de Silício/síntese química , Dióxido de Silício/efeitos da radiação , Dióxido de Silício/toxicidade , Ondas Ultrassônicas
17.
Chem Commun (Camb) ; 54(62): 8641-8644, 2018 Aug 11.
Artigo em Inglês | MEDLINE | ID: mdl-30020279

RESUMO

Herein, we report a facile, efficient and versatile method for the photo-regulation of pH-dependent activities of artificial enzymes by incorporating flash photolysis reagents. Under light excitation, a persistent pH shift is achieved by proton release from photosensitive 2-nitrobenzaldehyde. Following such change, the controlled activation of oxidase-like activity of nanoceria is successfully demonstrated.


Assuntos
Benzaldeídos/química , Materiais Biomiméticos/química , Materiais Biomiméticos/efeitos da radiação , Cério/química , Luz , Nanopartículas/química , Nanopartículas/efeitos da radiação , Biomimética , Concentração de Íons de Hidrogênio , Estrutura Molecular , Tamanho da Partícula , Propriedades de Superfície
19.
Int J Mol Sci ; 19(6)2018 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-29914214

RESUMO

Chitosan (CS) is a natural polymer derived from chitin that has found its usage both in research and commercial applications due to its unique solubility and chemical and biological attributes. The biocompatibility and biodegradability of CS have helped researchers identify its utility in the delivery of therapeutic agents, tissue engineering, wound healing, and more. Industrial applications include cosmetic and personal care products, wastewater treatment, and corrosion protection, to name a few. Many researchers have published numerous reviews outlining the physical and chemical properties of CS, as well as its use for many of the above-mentioned applications. Recently, the cationic polyelectrolyte nature of CS was found to be advantageous for stabilizing fascinating photonic materials including plasmonic nanoparticles (e.g., gold and silver), semiconductor nanoparticles (e.g., zinc oxide, cadmium sulfide), fluorescent organic dyes (e.g., fluorescein isothiocyanate (FITC)), luminescent transitional and lanthanide complexes (e.g., Au(I) and Ru(II), and Eu(III)). These photonic systems have been extensively investigated for their usage in antimicrobial, wound healing, diagnostics, sensing, and imaging applications. Highlighted in this review are the different works involving some of the above-mentioned molecular-nano systems that are prepared or stabilized using the CS polymer. The advantages and the role of the CS for synthesizing and stabilizing the above-mentioned optically active materials have been illustrated.


Assuntos
Técnicas Biossensoriais/métodos , Quitosana/análogos & derivados , Luz , Nanopartículas/química , Óptica e Fotônica/métodos , Quitosana/efeitos da radiação , Nanocompostos/química , Nanocompostos/efeitos da radiação , Nanopartículas/efeitos da radiação
20.
Biomater Sci ; 6(7): 1735-1744, 2018 Jun 25.
Artigo em Inglês | MEDLINE | ID: mdl-29808218

RESUMO

Bacterial infection is a major concern during the wound healing process. Herein, Ag/AgBr-loaded mesoporous silica nanoparticles (Ag/AgBr/MSNs) are designed to harvest visible light for rapid sterilization and acceleration of wound healing. The Ag/AgBr nanostructure has remarkable photocatalysis ability due to the critical factor that it can generate electron-hole pairs easily after light absorption. This remarkable photocatalytic effect enhances the antibacterial activity by producing reactive oxygen species (ROS). The bacterial killing efficiency of Ag/AgBr/MSNs is 95.62% and 99.99% against Staphylococcus aureus and Escherichia coli, respectively, within 15 min under simulated solar light irradiation due to the generation of ROS. Furthermore, the composites can arrest the bacterial growth and damage the bacterial membrane through electrostatic interaction. The gradual release of Ag+ not only prevents bacterial infection with good long-term effectiveness but also stimulates the immune function to produce a large number of white blood cells and neutrophils, which favors the promotion of the wound healing process. This platform provides an effective strategy to prevent bacterial infection during wound healing.


Assuntos
Antibacterianos/farmacologia , Brometos/farmacologia , Radiossensibilizantes/farmacologia , Compostos de Prata/farmacologia , Prata/farmacologia , Ferida Cirúrgica/tratamento farmacológico , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Brometos/química , Composição de Medicamentos/métodos , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Infecções por Escherichia coli/tratamento farmacológico , Infecções por Escherichia coli/metabolismo , Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/patologia , Luz , Masculino , Nanopartículas/química , Nanopartículas/efeitos da radiação , Nanopartículas/ultraestrutura , Porosidade , Radiossensibilizantes/química , Ratos , Ratos Wistar , Dióxido de Silício/química , Prata/química , Compostos de Prata/química , Oxigênio Singlete/análise , Oxigênio Singlete/metabolismo , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/metabolismo , Infecções Estafilocócicas/microbiologia , Infecções Estafilocócicas/patologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento , Ferida Cirúrgica/metabolismo , Ferida Cirúrgica/microbiologia , Ferida Cirúrgica/patologia
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