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1.
J Nanosci Nanotechnol ; 21(12): 6007-6015, 2021 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-34229798

RESUMO

Occupational exposure to indium oxide and indium containing particles has been associated with the development of severe lung diseases called "indium lung." According to the survey of occupational hygiene, indium oxide nanoparticles have been identified in the workplaces and the lungs of workers. To date, the potential mechanism of the pneumotoxicity has been poorly understood and no effective therapies are available against "indium lung." Our present study reported that the exposure of indium oxide nanoparticles damaged lung epithelial cells and alveolar macrophages and induced pulmonary alveolar proteinosis and inflammation in rats. In the 8-week post-exposure period, the indium oxide nanoparticles still mostly accumulated in the lungs and then persistently release indium ions in two months after exposure. In vitro, the epithelial cells show the greater potential for release of indium ions from indium oxide nanoparticles compared with the macrophages. EDTA-2Na, a metal chelating agent expected to remove the indium ions, was found to significantly reduced the cytotoxicity of indium oxide nanoparticles. Herein, the pneumotoxicity may be attributed to the slow and incremental release of indium ions from indium oxide nanoparticles primary dissolved by epithelial cells and macrophages, at least partially. The study may provide some insights to the pathogenicity mechanisms of "indium lung" and some clues against the health hazards of occupational inhaled indium oxide nanoparticles at the workplaces.


Assuntos
Índio , Nanopartículas , Animais , Células Epiteliais , Índio/toxicidade , Íons , Pulmão , Macrófagos , Nanopartículas/toxicidade , Ratos
2.
Int J Mol Sci ; 22(12)2021 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-34198694

RESUMO

Plasmonic nanoparticles are increasingly employed in several fields, thanks to their unique, promising properties. In particular, these particles exhibit a surface plasmon resonance combined with outstanding absorption and scattering properties. They are also easy to synthesize and functionalize, making them ideal for nanotechnology applications. However, the physicochemical properties of these nanoparticles can make them potentially toxic, even if their bulk metallic forms are almost inert. In this review, we aim to provide a more comprehensive understanding of the potential adverse effects of plasmonic nanoparticles in zebrafish (Danio rerio) during both development and adulthood, focusing our attention on the most common materials used, i.e., gold and silver.


Assuntos
Modelos Animais , Nanopartículas/toxicidade , Testes de Toxicidade , Animais , Tamanho da Partícula , Peixe-Zebra
3.
Analyst ; 146(15): 4945-4953, 2021 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-34259245

RESUMO

Two-photon fluorescence imaging is one of the most attractive imaging techniques for monitoring important biomolecules in the biomedical field due to its advantages of low light scattering, high penetration depth, and suppressed photodamage/phototoxicity under near-infrared excitation. However, in actual biological imaging, organic two-photon fluorescent dyes have disadvantages such as high biological toxicity and their fluorescence efficiency is easily affected by the complex environment in organisms. In this study, a novel nanoprobe platform with two-photon dye-doped silica nanoparticles was developed for FRET-based ratiometric biosensing and bioimaging, with endogenous ATP chosen as the target for detection. The nanoprobe has three components: (1) a two-photon dye-doped silica nanoparticle core, which serves as an energy donor for FRET; (2) amino-modified hairpin primers with carboxy fluorescein as an energy acceptor for FRET; (3) an aptamer acting as a recognition unit to realize the probing function. The nanoprobe showed ratiometric fluorescence responses for ATP detection with high sensitivity and high selectivity in vivo. Moreover, the nanoprobe showed satisfactory ratiometric two-photon fluorescence imaging of endogenous ATP in living cells and tissues (penetration depth of 190 nm). These results indicated that novel two-photon silica nanoparticles can be constructed by doping a two-photon fluorescent dye into silica nanoparticles, and they can effectively solve the disadvantages of two-photon fluorescent dyes. These excellent performances indicate that this novel nanoprobe platform will become a very valuable molecular imaging tool, which can be widely used in the biomedical field for drug screening and disease diagnosis and other related research.


Assuntos
Nanopartículas , Dióxido de Silício , Trifosfato de Adenosina , Transferência Ressonante de Energia de Fluorescência , Corantes Fluorescentes/toxicidade , Nanopartículas/toxicidade , Fótons , Dióxido de Silício/toxicidade
4.
Int J Mol Sci ; 22(11)2021 May 25.
Artigo em Inglês | MEDLINE | ID: mdl-34070324

RESUMO

Metal-organic frameworks (MOFs) demonstrate unique properties, which are prospective for drug delivery, catalysis, and gas separation, but their biomedical applications might be limited due to their obscure interactions with the environment and humans. It is important to understand their toxic effect on nature before their wide practical application. In this study, HKUST-1 nanoparticles (Cu-nanoMOF, Cu3(btc)2, btc = benzene-1,3,5-tricarboxylate) were synthesized by the microwave (MW)-assisted ionothermal method and characterized by X-ray powder diffraction (XRD) and transmission electron microscopy (TEM) techniques. The embryotoxicity and acute toxicity of HKUST-1 towards embryos and adult zebrafish were investigated. To gain a better understanding of the effects of Cu-MOF particles towards Danio rerio (D. rerio) embryos were exposed to HKUST-1 nanoparticles (NPs) and Cu2+ ions (CuSO4). Cu2+ ions showed a higher toxic effect towards fish compared with Cu-MOF NPs for D. rerio. Both forms of fish were sensitive to the presence of HKUST-1 NPs. Estimated LC50 values were 2.132 mg/L and 1.500 mg/L for zebrafish embryos and adults, respectively. During 96 h of exposure, the release of copper ions in a stock solution and accumulation of copper after 96 h were measured in the internal organs of adult fishes. Uptake examination of the major internal organs did not show any concentration dependency. An increase in the number of copper ions in the test medium was found on the first day of exposure. Toxicity was largely restricted to copper release from HKUST-1 nanomaterials structure into solution.


Assuntos
Cobre , Embrião não Mamífero/embriologia , Nanopartículas/toxicidade , Compostos Organometálicos , Peixe-Zebra/embriologia , Animais , Cobre/farmacocinética , Cobre/toxicidade , Compostos Organometálicos/farmacocinética , Compostos Organometálicos/toxicidade
5.
J Biomed Nanotechnol ; 17(6): 1123-1130, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34167626

RESUMO

Alzheimer's disease (AD) is strongly associated with oxidative stress which can damage neural cells. Silibinin has shown potential antioxidative effects. However, due to its low solubility in water, silibinin provides low biological activity and bioavailability. Therefore, to increase its pharmacological effects, silibilin was encapsulated into human serum albumin (HSA) nanoparticles and well-characterized by DLS and TEM techniques. The antioxidant activity of silibinin-HSA nanoparticles was evaluated on LPS-induced oxidative stress in neuron-like cells (SH-SY5Y) through MTT, antioxidant activity and apoptotic assay. It was shown that the mean diameter of HSA and silibinin-HSA nanoparticles were 88 and 105 nm, respectively with a drug loading of 24.08%, drug encapsulation rate of 94.72%, and the yield of silibinin-HSA nanoparticles of around 83.41% and the HSA nano-formulation released silibinin for 15 h. The results displayed that cell viability was reduced by LPS (10 µg/mL), who's also determined to stimulate oxidative stress and apoptosis. However, co-incubation of cells with silibinin (50 µg/mL) or silibinin-HSA nanoparticles led to the recovery of cell viability, activation of SOD and CAT, increase of GSH content, and reduction of ROS level, Caspase-3 activity and fragmentation of DNA. It was also indicated that the neuroprotective and antioxidant activities of silibinin-HAS nanoparticles was greater than free silibinin, indicating that using albumin can be a potential formulation approach for improving the antioxidant efficacy of silibinin.


Assuntos
Doença de Alzheimer , Nanopartículas , Silimarina , Albuminas , Doença de Alzheimer/tratamento farmacológico , Linhagem Celular Tumoral , Humanos , Nanopartículas/toxicidade , Estresse Oxidativo , Silibina , Silimarina/farmacologia
6.
Ecotoxicol Environ Saf ; 221: 112403, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34147863

RESUMO

The advent of the nanotechnology era offers a unique opportunity for sustainable agriculture provided that the exposure and toxicity are adequately assessed and properly controlled. The global production and application of cerium oxide nanoparticles (CeO2-NPs) in various industrial sectors have tremendously increased. Most of the nanoparticles end up in water and soil where they interact with soil microorganisms and plants. Investigating the uptake, translocation and accumulation of CeO2-NPs is critical for its safe application in agriculture. Plant uptake of CeO2-NPs may lead to their accumulation in different plant tissues and interference with key metabolic processes of plants. Soil microbes can also be affected by increasing CeO2-NPs in soil, leading to changes in the physiology and enzymatic activity of soil microorganisms. The interactions between CeO2-NPs, microbes and plants in the agricultural system need systemic research in ecologically relevant conditions. In the present review, The uptake pathways and in-planta translocation of CeO2-NPs,and their impact on plant morphology, nutritional values, antioxidant enzymes and molecular determinants are presented. The role of CeO2-NPs in modifying soil microbial community in plant rhizosphere is also discussed. Overall, the review aims to provide a comprehensive account on the behaviour of CeO2-NPs in soil-plant systems and their potential impacts on the soil microbial community and plant health.


Assuntos
Cério/toxicidade , Nanopartículas Metálicas/toxicidade , Microbiota/efeitos dos fármacos , Nanopartículas/toxicidade , Plantas/efeitos dos fármacos , Poluentes do Solo/toxicidade , Plantas/metabolismo , Microbiologia do Solo
7.
Ecotoxicol Environ Saf ; 221: 112436, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34171689

RESUMO

Nanoparticles (NPs), as a novel source of industrial materials, have been extensively used in recent years which ultimately ends up in soils and may cause toxic effects on plants. Gibberellic acid (GA), phytohormone, has ability to minimize abiotic stresses in plants. The role of GA in minimizing titanium dioxide (TiO2) NPs stress in plants is still unknown. In current study, soil was spiked with TiO2 NPs (0, 100, 200, 400, 600 mg/kg) while GA was foliar-sprayed at different concentrations during wheat growth. The findings revealed that TiO2 NPs increased the growth, chlorophyll contents, and nutrient (P, K, Fe, Mn) concentrations in tissues till 400 mg/kg and then decrease was observed at 600 mg/kg level of NPs whereas the values of these parameters were higher compared to control irrespective of NPs levels. The NPs enhanced the antioxidant activities (SOD, POD, CAT, APX) and reduced the oxidative stress (EL, H2O2, MDA) in leaves over the control. Foliar GA further improved the growth, yield, nutrients and antioxidant activities while minimized the oxidative stress compared to respective sole NPs- treatments. The interactive effects of NPs and GA were dose dependent. The results proved that studied doses of TiO2 NPs were not toxic to wheat plants except the highest level (600 mg/kg) used and GA positively affected the yield of wheat under TiO2 NPs application. The GA can be used to improve crop growth in the presence of NPs which, however, needs further investigation at higher doses of TiO2 NPs in various crops.


Assuntos
Giberelinas/farmacologia , Nanopartículas/toxicidade , Titânio/toxicidade , Triticum/efeitos dos fármacos , Antioxidantes/farmacologia , Transporte Biológico/efeitos dos fármacos , Minerais/metabolismo , Nutrientes/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Folhas de Planta/química , Folhas de Planta/metabolismo , Poluentes do Solo/toxicidade
8.
ACS Nano ; 15(6): 10640-10658, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34080832

RESUMO

Surface-modified mesoporous silica nanoparticles (MSNs) have attracted more and more attention as promising materials for biomolecule delivery. However, the lack of detailed evaluation relevant to the potential cytotoxicity of these MSNs is still a major obstacle for their applications. Unlike the bare MSNs and amino- or liposome-modified MSNs, we found that polyethylenimine-modified MSNs (MSNs-PEI) had no obvious toxicity to human umbilical vein endothelial cells (HUVECs) at the concentrations up to 100 µg/mL. However, MSNs-PEI induced autophagosomes accumulation by blocking their fusion with lysosomes, an essential mechanism for the cytotoxicity of many nanoparticles (NPs). Thus, we predicted that an alternative pathway for autophagosome clearance exists in HUVECs to relieve autophagic stress induced by MSNs-PEI. We found that MSNs-PEI prevented STX17 loading onto autophagosomes instead of influencing lysosomal pH or proteolytic activity. MSNs-PEI induced the structural alternation of the cytoskeleton but did not cause endoplasmic reticulum stress. The accumulated autophagosomes were released to the extracellular space via microvesicles (MVs) when the autophagic degradation was blocked by MSNs-PEI. More importantly, blockade of either autophagosome formation or release caused the accumulation of damaged mitochondria and excessive ROS production in the MSNs-PEI-treated HUVECs, which in turn led to cell death. Thus, we propose here that the MV-mediated autophagosome release, a compensation mechanism, allows the vascular endothelial cell survival when the degradation of autophagosomes is blocked by MSNs-PEI. Accordingly, promoting the release of accumulated autophagosomes may be a protective strategy against the endothelial toxicity of NPs.


Assuntos
Nanopartículas , Dióxido de Silício , Autofagossomos , Humanos , Lisossomos , Nanopartículas/toxicidade , Polietilenoimina , Porosidade
9.
Int J Mol Sci ; 22(10)2021 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-34065593

RESUMO

Interest in graphene oxide nature and potential applications (especially nanocarriers) has resulted in numerous studies, but the results do not lead to clear conclusions. In this paper, graphene oxide is obtained by multiple synthesis methods and generally characterized. The mechanism of GO interaction with the organism is hard to summarize due to its high chemical activity and variability during the synthesis process and in biological buffers' environments. When assessing the biocompatibility of GO, it is necessary to take into account many factors derived from nanoparticles (structure, morphology, chemical composition) and the organism (species, defense mechanisms, adaptation). This research aims to determine and compare the in vivo toxicity potential of GO samples from various manufacturers. Each GO sample is analyzed in two concentrations and applied with food. The physiological reactions of an easy model Acheta domesticus (cell viability, apoptosis, oxidative defense, DNA damage) during ten-day lasting exposure were observed. This study emphasizes the variability of the GO nature and complements the biocompatibility aspect, especially in the context of various GO-based experimental models. Changes in the cell biomarkers are discussed in light of detailed physicochemical analysis.


Assuntos
Materiais Biocompatíveis/química , Materiais Biocompatíveis/toxicidade , Grafite/química , Grafite/toxicidade , Animais , Apoptose/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Gryllidae/efeitos dos fármacos , Nanopartículas/química , Nanopartículas/toxicidade , Oxirredução/efeitos dos fármacos , Óxidos/metabolismo
10.
Langmuir ; 37(24): 7600-7610, 2021 06 22.
Artigo em Inglês | MEDLINE | ID: mdl-34115507

RESUMO

Cationic nanoparticles are known to interact with biological membranes and often cause serious membrane damage. Therefore, it is important to understand the molecular mechanism for such interactions and the factors that impact the degree of membrane damage. Previously, we have demonstrated that spatial distribution of molecular charge at cationic nanoparticle surfaces plays an important role in determining the cellular uptake and membrane damage of these nanoparticles. In this work, using diamond nanoparticles (DNPs) functionalized with five different amine-based surface ligands and small phospholipid unilamellar vesicles (SUVs), we further investigate how chemical features and conformational flexibility of surface ligands impact nanoparticle/membrane interactions. 31P-NMR T2 relaxation measurements quantify the mobility changes in lipid dynamics upon exposing the SUVs to functional DNPs, and coarse-grained molecular dynamics simulations further elucidate molecular details for the different modes of DNP-SUV interactions depending on the surface ligands. Collectively, our results show that the length of the hydrophobic segment and conformational flexibility of surface ligands are two key factors that dictate the degree of membrane damage by the DNP, while the amount of surface charge alone is not predictive of the strength of interaction.


Assuntos
Nanopartículas , Fosfolipídeos , Cátions , Ligantes , Bicamadas Lipídicas , Simulação de Dinâmica Molecular , Estrutura Molecular , Nanopartículas/toxicidade
11.
J Hazard Mater ; 415: 124907, 2021 08 05.
Artigo em Inglês | MEDLINE | ID: mdl-34088169

RESUMO

Nanoparticle-pollution has associated severe negative effects on crop productivity. Hence, methods are needed to alleviate nano-toxicity in crop plants. The present study aims to evaluate if the exogenous hydrogen sulfide (H2S) application in combination with silicon (Si) could palliate the harmful effects of copper oxide nanoparticles (CuO NPs). Fifteen day-old rice (Oryza sativa L.) seedlings were used as a model plant. The results indicate that simultaneous exogenous addition of 10 µM Si and 100 µM NaHS (as an H2S donor) provided tolerance and enhanced defence mechanism of the rice seedlings against 100 µM CuO NPs. Thus, it was observed in terms of their growth, photosynthetic pigments, antioxidant enzyme activities, the content of non-enzymatic components, chlorophyll fluorescence and up-regulation of antioxidant genes. Si and NaHS stimulated gene expression of silicon (Lsi1 and Lsi2) and auxin (PIN5 and PIN10) transporters. Taken together, data indicate that H2S underpins the beneficial Si effects in rice seedlings against the oxidative stress triggers by CuO NPs, and stimulation of enzymatic components of the ascorbate-glutathione cycle being the main factor for the beneficial effects triggered by the couple of Si and H2S. Therefore, it could be concluded that the simultaneous application of Si and H2S promote the resilience of the rice seedlings against the oxidative stress induced by CuO NPs.


Assuntos
Sulfeto de Hidrogênio , Nanopartículas , Oryza , Cobre/toxicidade , Sulfeto de Hidrogênio/toxicidade , Nanopartículas/toxicidade , Óxidos , Plântula , Silício/toxicidade
12.
Sci Total Environ ; 778: 146446, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34030365

RESUMO

The presence of extracellular polymeric substance (EPS) plays a vital role in the accumulation and toxicity of nanoparticles to microorganisms, in which the involved processes and mechanisms are still waiting to be revealed. Herein, we specifically investigated the interfacial interaction between titanium dioxide nanoparticles (nTiO2) and algae (Chlorella pyrenoidosa) with/without EPS and the effect of EPS on algal cell internalization of nTiO2. Results showed that the presence of EPS on cell surface promoted heteroaggregation between nTiO2 and algal cells, and induced more nTiO2 accumulation on algal surface; however, algal cell internalization of nTiO2 was limited by the presence of EPS. Pearson correlation analysis further proved that the presence of EPS had a positive effect on the surface accumulation of nTiO2 and a negative effect on the internalization of nTiO2. More than 60% of cell internalized nTiO2 entered algal cells through the energy dependent endocytosis pathway. It is interesting to find that anatase nTiO2 (nTiO2-A) entered algal cells mainly through the clathrin dependent endocytosis, while rutile nTiO2 (nTiO2-R) mainly through the dynamin dependent endocytosis. This difference could be due to the different affinities of nTiO2-A and nTiO2-R to the mediating receptors referring to different endocytic pathways. The removal of EPS activated the associated mediating pathways, allowing more nTiO2 to be internalized. These findings address the role of EPS on the interaction between nTiO2 and algae and promote a deeper understanding of the ecological effect of nTiO2.


Assuntos
Chlorella , Nanopartículas , Matriz Extracelular de Substâncias Poliméricas , Nanopartículas/toxicidade , Titânio/toxicidade
13.
Nanoscale ; 13(20): 9415-9435, 2021 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-34002735

RESUMO

AIM: In this study, the influence of a serum albumin (SA) and human plasma (HP) derived protein- and lipid molecule corona on the toxicity and biodegradability of different iron oxide nanoparticles (IONP) was investigated. METHODS: IONP were synthesized and physicochemically characterized regarding size, charge, and colloidal stability. The adsorbed proteins were quantified and separated by gel electrophoresis. Adsorbed lipids were profiled by ultraperformance liquid chromatography-ESI-tandem mass spectrometry. The biocompatibility was investigated using isolated erythrocytes and a shell-less hen's egg model. The biodegradability was assessed by iron release studies in artificial body fluids. RESULTS: The adsorption patterns of proteins and lipids varied depending on the surface characteristics of the IONP like charge and hydrophobicity. The biomolecule corona modified IONP displayed favorable colloidal stability and toxicological profile compared to IONP without biomolecule coronas, reducing erythrocyte aggregation and hemolysis in vitro as well as the corresponding effects ex ovo/in vivo. The coronas decreased the degradation speed of all tested IONP compared to bare particles, but, whereas all IONP degraded at the same rate for the SA corona, substantial differences were evident for IONP with HP-derived corona depending on the lipid adsorption profile. CONCLUSION: In this study the impact of the proteins and lipids in the biomolecule corona on the entire IONP application cycle from the injection process to the degradation was demonstrated.


Assuntos
Nanopartículas , Coroa de Proteína , Animais , Galinhas , Feminino , Humanos , Lipídeos , Nanopartículas Magnéticas de Óxido de Ferro , Nanopartículas/toxicidade
14.
Sci Total Environ ; 786: 147402, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-33975099

RESUMO

Heavy metal and nanoparticles (NPs) emitted in the environment have attracted worldwide attention. But the combined effect of NPs and heavy metals is still unclear. In this study, the combined effect of zinc-based NPs and Cd2+ on HepG2 cells was investigated by combining biological indicator detection methods with time-resolved inductively coupled plasma mass spectrometry (TRA-ICP-MS) single cell analysis, and the combined effect of Zn2+ and Cd2+ was also investigated for a comparison. High-dose of ZnO or ZIF-8 NPs co-exposure with Cd2+ would reduce the cell viability while low-dose of ZnO or ZIF-8 NPs co-exposure with Cd2+showed antagonism and the particle size has no remarkable effect on the combined toxicity. In the antagonism, Zn2+ would increase cellular Zn amount through increasing the expression of ZIP8 and ZIP14 transporters to manage the ROS generation, but the zinc-based NPs would decrease expression of these transporters to decrease cellular Cd amount to help maintain the cell viability. Thus, we should hold a dialectical thinking about the pollution of NPs emissions in the environment.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Óxido de Zinco , Cádmio/toxicidade , Células Hep G2 , Nanopartículas Metálicas/toxicidade , Nanopartículas/toxicidade , Zinco , Óxido de Zinco/toxicidade
15.
J Nanosci Nanotechnol ; 21(11): 5449-5458, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-33980355

RESUMO

This research aimed to prepare 166Dy2O3-iPSMA/166Ho2O3-iPSMA nanoparticles (166Dy2O3/166Ho2O3-iPSMA NPs) and assess the radiation absorbed dose produced by the nanosystem to hepatic cancer cells by using experimental in vitro and in vivo biokinetic data. Dy2O3NPs were synthesized and functionalized with the prostate-specific membrane antigen inhibitor peptide (iPSMA). Fourier transform infrared (FTIR) spectroscopy, transmission electron microscope (TEM), dynamic light scattering (DSL) and zeta potential analyses indicated the formation of Dy2O3-iPSMA NPs (46.11 ± 13.24 nm). After neutron activation, a stable 166Dy2O3/166Ho2O3- iPSMA nanosystem was obtained, which showed adequate affinity to the PSMA receptor in HepG2 cancer cells (Kd = 9.87 ± 2.27 nM). in vitro studies indicated high 166Dy2O3/166Ho2O3-iPSMA internalization in cancer cells, with high radiation doses to cell nuclei (107 Gy) and cytotoxic effects, resulting in a significant reduction in HepG2 cell viability (decreasing to 2.12 ± 0.31%). After intratumoral administration in mice, the nanosystem biokinetic profile indicated significant retention into the tumoral mass, producing ablative radiation doses (>70 Gy).


Assuntos
Nanopartículas , Animais , Linhagem Celular Tumoral , Masculino , Camundongos , Nanopartículas/toxicidade , Espectroscopia de Infravermelho com Transformada de Fourier
16.
Aquat Toxicol ; 236: 105867, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34052720

RESUMO

Cerium oxide nanoparticles (nCeO2) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO2 on tested animals, but literature data are mainly contradictory. Coating of nCeO2 can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO2. Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO2 in comparison to uncoated nCeO2 using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO2 on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO2 on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO2 on Vibrio fischeri. The coating of nCeO2 did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO2, while the crustacean's O2 consumption was not affected by any of the coated nCeO2. In summary, this study revealed that, at 200 mg L-1, uncoated and carbohydrate-coated nCeO2 are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO2. The highest production was in glucose and levan-coated nCeO2 according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri).


Assuntos
Cério/toxicidade , Nanopartículas/toxicidade , Poluentes Químicos da Água/toxicidade , Aliivibrio fischeri/efeitos dos fármacos , Animais , Organismos Aquáticos , Daphnia/efeitos dos fármacos , Frutanos/farmacologia , Glucanos , Glucose , Peixe-Zebra
17.
Sci Total Environ ; 773: 144895, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33940706

RESUMO

Titanium dioxide nanoparticles (TiO2 NPs) are widely used as nano-agrochemicals. In this study we investigated the influence of soil heterogeneity on bacterial communities exposed to TiO2 NPs over time. Clay and sandy soils with low- and high-organic matter contents were exposed to environmentally relevant concentration of TiO2 NPs (1 mg/kg) and soil bacterial communities were sampled after short-term (15 days) and long-term exposure (60 days). After short-term TiO2 NPs exposure, significant effects regarding the enzyme activity, bacterial community structure and composition, and community functioning were observed in the clay soils with high organic matter (clay-HOM) but not in other soil groups. Response alterations were observed to taxa belonging to Acidobacteria and Verrucomicrobia, and functional pathways related to carbohydrates degradation. These results indicated that soil heterogeneity play more important roles in shaping the bacterial community in soil with low clay fraction and less organic matter, while TiO2 NPs selection was the main driver in inducing the compositional and functional impacts on the soil bacterial community in the presence of clay soil with high organic matter content. As exposure time increased, the bacterial community recovered after a long-term exposure of 60 days, suggesting that the bacterial evolution and adaptation could overcome the TiO2 NPs selection after long-term exposure. Our results highlighted the importance of soil heterogeneity including clay fraction and organic matter and exposure duration in assessing the impact of nanoparticle on soil bacterial activity, community and function. By comprehensively evaluating the risks of nanoparticles on soil ecosystem and explicitly and explicitly include spatial and temporal variations, the benefit of nano-agrochemical products has the potential to be promoted in future applications.


Assuntos
Nanopartículas , Solo , Ecossistema , Nanopartículas/toxicidade , Microbiologia do Solo , Titânio
18.
Sci Total Environ ; 773: 145078, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33940715

RESUMO

Nanomaterials are increasingly used in food processing, daily necessities and other fields due to their excellent properties, and increase the environmental contamination. Human beings will inevitably come into contact with these nanomaterials through multiple exposure routes especially oral exposure. The intestine is an important organ for nutrient absorption and physiologic barrier, which may be the main target of nanoparticles (NPs) exposure. However, for a long time, research on the toxicity of NPs has mainly focused on organs such as liver, kidney and brain. There are few assessment data over the intestinal safety. Recently, as reported, NPs can be translocated to the intestinal part in mammals and would be distributed in different substructures of intestines, thus causing damage to the structure and function of the intestine, in which the gut microbiota and its metabolites play important roles. In addition, due to the special physiological environment of gut, nanomaterials will undergo complex transformations that may cause different biological effects from their original form. Therefore, this review aims to assess the potential adverse effects of NPs on intestine and its possible mechanisms through the results of in vivo mammalian experiments. In addition, the exposure pathway, biodistribution and biotransformation of NPs in the intestine are also considered. We hope this review will arouse people's attention to the intestinal nanotoxicology and provide basic information for further related studies.


Assuntos
Microbioma Gastrointestinal , Nanopartículas , Animais , Humanos , Fígado/metabolismo , Mamíferos , Nanopartículas/toxicidade , Distribuição Tecidual
19.
Sci Total Environ ; 773: 145442, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33940727

RESUMO

Cadmium (Cd) has been confirmed as an environmental contaminant, which potential threats health impacts to humans and animals. Selenium (Se) as a beneficial element that alleviates the negative effects of Cd toxicity. Se mainly exists in two forms in food nutrients including organic Se usually as (Se-enriched yeast (SeY)) and inorganic Se (sodium selenite (SSe)). Nanoparticle of Se (Nano-Se), a new form Se, which is synthesized by the bioreduction of Se species, which attracted significant attention recently. However, compared the superiority alleviation effects of Nano-Se, SeY or SSe on Cd-induced toxicity and related mechanisms are still poorly understood. The purpose of this study was to compare the superiority antagonism effects of Nano-Se, SeY and SSe on Cd-induced inflammation response via NF-kB/IκB pathway in the heart. The present study demonstrated that exposed to Cd obviously increased the accumulation of Cd, disruption of ion homeostasis and depressed the ratios of K+/Na+ and Mg2+/Ca2+ via ion chromatography mass spectrometry (ICP-MS) detecting the heart specimens. In the results of histological and ultrastructure observation, typical inflammatory infiltrate characteristics and mitochondria and nuclear structure alterations in the hearts of Cd group were confirmed. Cd treatment enhanced the inducible nitric oxide synthase (iNOS) activities and NOS isoforms expression via NF-kB/IκB pathway to promote inflammation response. However, the combined treatment of Cd-exposed animals with Nano-Se was more effective than SeY and SSe in reversing Cd-induced histopathological changes and iNOS activities increased, reducing Cd accumulation and antagonizing Cd-triggered inflammation response via NF-kB/IκB pathway in chicken hearts. Overall, Se applications, especially Nano-Se, can be most efficiently used for relieving cardiotoxicity by exposed to Cd compared to other Se compound.


Assuntos
Nanopartículas , Selênio , Animais , Cádmio/toxicidade , Humanos , Inflamação/induzido quimicamente , NF-kappa B , Nanopartículas/toxicidade , Saccharomyces cerevisiae , Selenito de Sódio
20.
Ecotoxicol Environ Saf ; 219: 112312, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33989917

RESUMO

Copper (Cu) pollution is common in the soil. Due to the widespread application of TiO2 NPs, there is a high propensity for the co-occurrence of TiO2 nanoparticles (NPs) and Cu in agricultural soils. It is therefore imperative to evaluate the joint effects of TiO2 NPs and Cu on crops. In this study, the mutual effects of TiO2 NPs and Cu on their toxicity and accumulation in soybean seedlings and on their fates in a hydroponic system were determined. When Cu was at levels of 1 and 2 mg/L, the co-occurring TiO2 NPs at a non-toxic concentration (10 mg/L) significantly enhanced the toxicity and accumulation of Cu and Ti in soybeans, and inhibited the translocation of Cu from soybean roots to shoots. However, when the Cu concentration for co-exposure was ≥ 5 mg/L, such mutual effects disappeared. The amount of Cu ions adsorbed onto TiO2 NPs after 48 h of co-exposure gradually increased from 31 to 118 mg/g when the Cu concentration was increased from 1 to 20 mg/L. The aggregation and sedimentation of TiO2 NPs were significantly increased after 48 h of co-exposure with the Cu at a concentration higher than 5 mg/L, as compared to the single TiO2 NPs exposure. The increasing aggregation and sedimentation might reduce the bioavailability of TiO2 NPs associated with the adsorbed Cu to soybeans, and consequently alleviate or even neutralize the enhanced toxicity and accumulation of Cu in soybeans exerted by the co-existing TiO2 NPs. Our results thus suggest that consideration of the impact of TiO2 NPs on the phytotoxicity of heavy metals, and specifically Cu, needs to be interpreted with care, and highlight the importance of integrating the interaction and fates of TiO2 NPs and metals into their risk assessment.


Assuntos
Cobre/metabolismo , Nanopartículas/toxicidade , Titânio/toxicidade , Adsorção , Disponibilidade Biológica , Cobre/toxicidade , Produtos Agrícolas , Fabaceae , Hidroponia , Metais Pesados/farmacologia , Raízes de Plantas/efeitos dos fármacos , Plântula/efeitos dos fármacos , Solo , Soja
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