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1.
Food Chem ; 299: 125108, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31310914

RESUMO

Macroporous resins have been employed in the effective recovery of flavonoids from plants. In this study, S8 polar resins were used to recover flavonoids and procyanidins from lotus seedpods. Adsorption kinetics, isotherms, and thermodynamics studies revealed that the adsorption process involved physico-chemical interactions, including flavonoid-resin and flavonoid-flavonoid electrostatic interactions, π-π aromatic stacking, moderate and strong hydrogen bonding, and repulsive forces. These forces worked complementarily in adsorption, except for the repulsive force, which opposed the adsorption. Further, adsorption temperature determined the adsorption behavior, with multilayer adsorption enhancing adsorption capacity. In dynamic desorption tests, an acetone/water/acetic acid mixture (58.77: 39.34: 1.89) designed by the D-optimal design method was able to desorb 95.57% and 89.85% of total flavonoids and procyanidins, respectively, using less than two bed volumes of solvent. Ultra-performance liquid chromatography triple-time of flight/mass spectrometry (UPLC-TOF/MS) analysis showed that 26 flavonoids, including 5 procyanidins, were detected after the recovery.


Assuntos
Flavonoides/isolamento & purificação , Nelumbo/química , Resinas Sintéticas/química , Sementes/química , Adsorção , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Flavonoides/química , Ligações de Hidrogênio , Espectrometria de Massas , Proantocianidinas/análise , Proantocianidinas/química , Proantocianidinas/isolamento & purificação , Eletricidade Estática
2.
J Agric Food Chem ; 67(28): 7855-7868, 2019 Jul 17.
Artigo em Inglês | MEDLINE | ID: mdl-31274310

RESUMO

Bee pollen (BP) collected from different floras possesses various potential bioactivities, but the mechanism-related research on anti-inflammatory effects is limited. Here, three types of BP originating from Camellia sinensis L. (BP-Cs), Nelumbo nucifera Gaertn. (BP-Nn), and Brassica campestris L. (BP-Bc) were assessed using molecular and metabolomics methods to determine their anti-inflammatory effects. The differences in polyphenolic abundance of three types of BP extracts were determined by HPLC-DAD/Q-TOF-MS. In vitro anti-inflammatory effects of three BP extracts were evaluated in a lipopolysaccharide (LPS)-induced RAW 264.7 cells model. BP-Cs extract with the most abundant polyphenols was found to be the most effective in reducing inflammation by downregulating inflammatory-related genes expression and blocking the activation of MAPK and NF-κB signaling pathways. Polyphenol-rich BP-Cs was further evaluated for their in vivo anti-inflammatory effect in a LPS-induced acute lung injury mouse model. An UPLC-Q-TOF/MS-based metabolomics approach was applied to analyze metabolite changes in mouse serum. Weshowed that the pretreated BP-Cs extract alleviated inflammation and regulated glycerophospholipid metabolism significantly. Our findings provide a foundation for developing and justifying BP as a potential anti-inflammatory ingredient in functional foods or nutraceutical formulations.


Assuntos
Lesão Pulmonar Aguda/tratamento farmacológico , Anti-Inflamatórios/administração & dosagem , Extratos Vegetais/administração & dosagem , Pólen/química , Lesão Pulmonar Aguda/genética , Lesão Pulmonar Aguda/imunologia , Animais , Anti-Inflamatórios/química , Abelhas , Brassica/química , Camellia sinensis/química , Cromatografia Líquida de Alta Pressão , Humanos , Lipopolissacarídeos/efeitos adversos , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Masculino , Espectrometria de Massas , Camundongos , Camundongos Endogâmicos ICR , Quinases de Proteína Quinase Ativadas por Mitógeno/genética , Quinases de Proteína Quinase Ativadas por Mitógeno/imunologia , NF-kappa B/genética , NF-kappa B/imunologia , Nelumbo/química , Extratos Vegetais/química , Polifenóis/administração & dosagem , Polifenóis/química , Células RAW 264.7
3.
Molecules ; 24(9)2019 Apr 26.
Artigo em Inglês | MEDLINE | ID: mdl-31027372

RESUMO

Nelumbo nucifera Gaertn. (lotus) is an important medicinal plant, and many parts of the plant have been investigated for their therapeutic effects. However, the therapeutic effect of receptacles of lotuses on pathological cardiomyocyte hypertrophy has not been investigated yet. Therefore, the current study aimed to determine the protective effect of lotus against angiotensin II (Ang II)-induced cardiomyocyte hypertrophy in vitro. Ang II was used to induce hypertrophy of H9c2 cells. The lotus receptacle powder (MeOH extract of receptaculum Nelumbinis; MRN) used in the experiments was prepared by MeOH extraction and subsequent evaporation. To evaluate the effect of MRN on cardiomyocyte hypertrophy, cell size, protein synthesis, and hypertrophic marker expressions were examined. The antioxidant ability of MRN was determined by using CM-H2DCFDA, a general oxidative stress indicator. Ang II-induced cardiomyocyte hypertrophy was significantly attenuated by 5 µg/mL of MRN, as confirmed by the reductions in cell size, protein synthesis, and hypertrophic marker expression. MRN also attenuated Ang II-induced excessive intracellular reactive oxygen species (ROS) production through the suppression of protein kinase C (PKC), extracellular-signal-regulated kinase (ERK), and NF-κB activation and subsequent type I angiotensin receptor (AT1R), receptor for advanced glycation end products (RAGE), and NADPH oxidase (NOX) expression. MRN exerted a significant protective effect against Ang II-induced cardiomyocyte hypertrophy through suppression of PKC-ERK signaling, and this subsequently led to attenuation of intracellular ROS production.


Assuntos
Angiotensina II/metabolismo , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Nelumbo/química , Extratos Vegetais/farmacologia , Angiotensina II/farmacologia , Animais , Biomarcadores , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Hipertrofia , Miócitos Cardíacos/patologia , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Proteína Quinase C/metabolismo , Ratos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais/efeitos dos fármacos
4.
Sci Total Environ ; 666: 865-874, 2019 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-30818210

RESUMO

Developing a highly efficient, cost-effective, easily scalable and sustainable cathode for oxygen reduction reaction (ORR) is a crucial challenge in terms of future "green" energy conversion technologies, e.g., microbial fuel cells (MFCs). In this study, a natural and widely available lotus leaf with intrinsically hierarchical structure was employed to serve as the single precursor to prepare the catalyst applied as the MFC cathode. The hierarchically particle-coated bio­carbon was self-constructed from the lotus leaf, which yielded a large specific surface area, highly porous structure and superhydrophobicity via facile pyrolysis coupling hydrothermal activation by ZnCl2/(NH4)2SO4. Electrochemical evaluation demonstrated that these natural leaf-derived carbons have an efficient ORR activity. Specifically, the HC-900 catalyst with hydrothermal activation achieved an onset potential of -0.015 V vs. Ag/AgCl, which was comparable to the commercial Pt/C catalyst (-0.010 V vs. Ag/AgCl) and was more efficient than the DC-900 catalyst through direct pyrolysis. Furthermore, the HC-900 catalyst achieved an outstanding ORR activity via a one-step and four-electron pathway, exhibiting a potential alternative to Pt/C as electrocatalyst in ORR, due to its better long-term durability and methanol resistance. Additionally, the HC-900 catalyst was applied as an effective electrocatalytic cathode in an MFC system with a maximum power density of 511.5 ±â€¯25.6 mW⋅m-2, exhibiting a superior energy harvesting capacity to the Pt/C cathode.


Assuntos
Fontes de Energia Bioelétrica , Carbono/química , Nanoestruturas/química , Nelumbo/química , Catálise , Técnicas Eletroquímicas , Folhas de Planta/química
5.
Food Chem ; 286: 449-458, 2019 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-30827632

RESUMO

The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch. The result showed that water content affected the composition of resistant starch. The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure. Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal. Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found. Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure. Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.


Assuntos
Modelos Teóricos , Nelumbo/química , Amido/química , Água/análise , Espectroscopia de Ressonância Magnética , Sementes/química , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
6.
Food Chem ; 274: 933-941, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30373030

RESUMO

Lotus seed kernel (LSK) has high starch content and possesses many bioactivities, which are mainly attributed to its phenolics. In the present study, LSK phenolics were extracted using aqueous glycerol, and the obtained phenolics and its starch properties were observed for their correlations. Response surface methodology indicated significant quadratic effect of temperature and significant linear effect of glycerol on LSK phenolics (p < 0.05). Temperature also had a significant effect on LSK starch solubility and swelling power and % leaching amylose (p < 0.05). Leaching amylose in the presence of glycerol and swelling power showed positive correlations with LSK phenolics in a linear relationship (r = 0.825, p < 0.01) below 50 °C and a monotonic relationship (rs = 0.933, p < 0.01) above 60 °C, respectively. A building block backbone model was adopted to illustrate the interaction between amylose/amylopectin-glycerol, and most importantly, their relationship to LSK phenolics.


Assuntos
Amilopectina/química , Amilose/química , Nelumbo/química , Fenóis/isolamento & purificação , Amido/química , Fracionamento Químico/métodos , Glicerol/química , Sementes/química , Solubilidade , Temperatura Ambiente
7.
Biol Pharm Bull ; 41(3): 399-408, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29491217

RESUMO

The sleep-promoting effects of the water extract of Nelumbo nucifera seeds (NNE) were investigated in an invertebrate model. The effects of NNE on the subjective nighttime activity, sleep episodes, and sleep time were determined using Drosophila melanogaster and locomotor activity monitoring systems in basal and caffeine-induced arousal conditions. The movements of fruit flies were analyzed using the Noldus EthoVision-XT system, and the levels of neuromodulators were analyzed using HPLC. Expression of neuromodulator receptors was analyzed using real-time PCR. NNE was shown to contain neurotransmission-related components; γ-aminobutyric acid (GABA) (2.33±0.22 mg/g), tryptophan (2.00±0.06 mg/g), quinidine (0.55±0.33 mg/g), and neferine (0.16±0.01 mg/g). The total activity of flies during nighttime was decreased by 52% with 1.0% NNE treatment. In the individual and collective conditions, the subjective nighttime activities (45/38%) and sleep bouts (20/14%) of flies was significantly decreased with NNE treatment, while total sleep times (10/27%) were significantly increased. This sleep-promoting effect is more pronounced in caffeine-treated conditions; the nighttime activity of flies was reduced by 53%, but total sleep time was increased by 60%. Our video-tracking analysis showed a significant decrease of the moving distance and velocity of flies by NNE. This NNE-mediated sleep-promoting effect was associated with up-regulation of GABAA/GABAB and serotonin receptors. The NNE-mediated increase of GABA content was identified in flies. These results demonstrate that NNE effectively promotes sleep in flies by regulating the GABAergic/serotonergic neuromodulators, and could be an alternative agent for sleep promotion.


Assuntos
Nelumbo/química , Extratos Vegetais/farmacologia , Sementes/química , Sono/efeitos dos fármacos , Animais , Comportamento Animal/efeitos dos fármacos , Cafeína/farmacologia , Estimulantes do Sistema Nervoso Central/farmacologia , Drosophila melanogaster , Atividade Motora/efeitos dos fármacos , Neurotransmissores/metabolismo , Receptores de GABA-A/efeitos dos fármacos , Receptores de GABA-B/efeitos dos fármacos , Receptores de Neurotransmissores/efeitos dos fármacos , Receptores de Serotonina/efeitos dos fármacos
8.
Int J Biol Macromol ; 113: 219-226, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29476856

RESUMO

The present study aimed to investigate the influence of microwave irradiation on the physical and functional properties of starch extracted from Nelumbo nucifera seed flour. The seed flour was obtained by manual grinding of seeds and irradiated at different microwave treatment time, i.e., 1, 2, 3, 4 and 5min at the low-medium intensity. The starch was extracted in distilled water and dried at room temperature using a vacuum desiccator. The morphology, crystal structure, and surface parameters of starch granules were analyzed using scanning electron microscopy, X-ray diffraction, Fourier transform infra-red spectroscopy and Brunauer, Emmett and Teller surface analysis techniques. The functional properties of starch were also determined in terms of water and oil holding capacity, swelling capacity, emulsifying activity and gelling ability. Evidently, crystallinity, surface area and pore volume of starch granules were found to be increased, while particle size and average pore size were decreased as a function of microwave treatment time. The microwave-induced variations in physical parameters significantly affected the functional properties of starch. A significant (p>0.05) exponential decrease in extraction yield and gelling ability, while an exponential increase in the functional properties of starch with increase in the microwave treatment time (R2=0.915-0.985) was recorded.


Assuntos
Farinha , Temperatura Alta , Micro-Ondas , Nelumbo/química , Sementes/química , Amido/química , Amido/isolamento & purificação , Manipulação de Alimentos , Solubilidade , Propriedades de Superfície
9.
Fitoterapia ; 125: 184-190, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29371159

RESUMO

Chemical investigation of the embryos of Nelumbo nucifera afforded four new flavone C-glycosides, named nelumbosides A-D (1-4), together with nine known ones, comprising five flavonoids (5-9) and four alkaloids (10-13). The chemical structures of the new compounds were elucidated by 1D, 2D-NMR and HR-ESI-MS techniques, together with chemical methods. Nelumbosides A-D (1-4) are rarely present in naturally occurring flavone C-glycosides featuring a 4-hydroxystyrene unit connected to the flavonoid skeleton. Compounds 2-13 were evaluated for their antioxidant activity by ABTS and DPPH radical-scavenging assay. Among them, compounds 2, 6, 7 and 11 exhibited strong scavenging activity with SC50 values ranging from 12.07 to 25.68µM compared with the positive control l-ascorbic acid.


Assuntos
Alcaloides/química , Antioxidantes/química , Flavonoides/química , Glicosídeos/química , Nelumbo/química , Alcaloides/isolamento & purificação , Antioxidantes/isolamento & purificação , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Estrutura Molecular , Sementes/química
10.
J Med Food ; 21(2): 203-206, 2018 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29356593

RESUMO

The protective activity of a mixture of aqueous and ethanolic extracts from Houttuynia cordata Thunb, Nelumbo nucifera G. leaves, and Camellia sinensis seed (HNC) was evaluated in C57BL/6 mice. Pretreatment with HNC prevented the elevation of serum aspartate aminotransferase and alanine aminotransferase caused by ethanol-induced hepatic damage. The HNC-treated mice showed significantly lower triglyceride levels, reduced CYP2E1 activity, and increased antioxidant enzyme activities and lipogenic mRNA levels. These results suggest that HNC might be a candidate agent for liver protection against ethanol-induced oxidative damage, through enhancement of antioxidant and antilipogenic activity.


Assuntos
Camellia sinensis/química , Etanol/toxicidade , Houttuynia/química , Hepatopatias/prevenção & controle , Nelumbo/química , Extratos Vegetais/administração & dosagem , Substâncias Protetoras/administração & dosagem , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Humanos , Fígado/efeitos dos fármacos , Fígado/metabolismo , Hepatopatias/sangue , Hepatopatias/etiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Extratos Vegetais/isolamento & purificação , Substâncias Protetoras/isolamento & purificação , Triglicerídeos/metabolismo
11.
Int J Biol Macromol ; 111: 660-666, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29320727

RESUMO

From the bee pollen of Nelumbo nucifera, we used hot water extraction and chromatographic fractionation on DEAE-cellulose and Sepharose CL-6B to purify polysaccharides (WNPP) to homogeneity. WNPP-2-RG (3.8 × 105 Da) consisted primarily of Rha (11.5%), GalA (12.0%), Gal (41.2%) and Ara (29.7%). Structure analysis showed that this polysaccharide is a RG-I type pectin containing AG-I and AG-II side chains comprised of 1,5-L-Ara (25.6%), t-D-Gal (12.0%), and 1,6-D-Gal (18.3%) as the primary linkage types. Immunological activity assays demonstrated that WNPP-2-RG increased lymphocyte proliferation and macrophage phagocytosis, and induced production of NO. Compared to WNPP-2-RG, its hydrolysates (RG-8H-P) had no effect on lymphocyte proliferation, although they did promote macrophage phagocytosis and decreased NO production. Our results suggest that Gal side chain residues may play a crucial role in macrophage phagocytosis, whereas Ara is unimportant for NO production and neither Gal nor Ara impact lymphocyte proliferation.


Assuntos
Pectinas/química , Pólen/química , Polissacarídeos/química , Animais , Abelhas/química , Proliferação de Células/efeitos dos fármacos , Humanos , Linfócitos/efeitos dos fármacos , Linfócitos/imunologia , Macrófagos/efeitos dos fármacos , Nelumbo/química , Nelumbo/imunologia , Pectinas/imunologia , Pectinas/farmacologia , Fagocitose/efeitos dos fármacos , Pólen/imunologia , Polissacarídeos/imunologia , Polissacarídeos/farmacologia , Relação Estrutura-Atividade
12.
Planta Med ; 84(2): 83-90, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28817840

RESUMO

This study aimed to elucidate the mechanisms of nuciferine (a main aporphine alkaloid of lotus leaf extract), which can induce relaxation in contracted tracheal rings. Under Ca2+-free and 2 mM Ca2+ conditions, we found that nuciferine had no effect on the resting muscle tone of tracheal rings. In contrast, nuciferine relaxed high K+-contracted mouse tracheal rings in a dose-dependent manner and inhibited both Ca2+ influx and voltage-dependent L-type Ca2+ channel currents induced by high K+. Similarly, nuciferine also inhibited acetylcholine-induced contractions in mouse tracheal rings in a dose-dependent manner. Meanwhile, both acetylcholine-induced intracellular Ca2+ influx and whole-cell currents of nonselective cation channels were blocked by nuciferine. Together, the results indicate that nuciferine-induced relaxation in tracheal rings mainly occurred due to the inhibition of extracellular Ca2+ influx through the blockade of voltage-dependent L-type Ca2+ channels and/or nonselective cation channels. These results suggest that nuciferine has a therapeutic effect on respiratory diseases associated with the aberrant contraction of airway smooth muscles and/or bronchospasm.


Assuntos
Aporfinas/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Relaxamento Muscular/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Nelumbo/química , Parassimpatolíticos/farmacologia , Traqueia/efeitos dos fármacos , Acetilcolina/metabolismo , Animais , Aporfinas/química , Aporfinas/metabolismo , Cálcio/metabolismo , Canais de Cálcio/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Estrutura Molecular , Músculo Liso/metabolismo
13.
J Chromatogr Sci ; 56(2): 108-114, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-29045617

RESUMO

High-speed counter-current chromatography (HSCCC) combined with macroporous resin (MR) column was successfully applied to the isolation and purification of four flavonoid glycosides from the medicinal herb Lotus plumule (LP). A polar two-phase solvent system composed of ethyl acetate-n-butanol-water (1:2:3, v/v/v) was selected by high-performance liquid chromatography (HPLC) and run on a preparative scale where the lower aqueous phase was used as the mobile phase with a head-to-tail elution mode. Quercetin-3-O-ß-D-glucopyranoside (15 mg), isorhamnetin-3-O-ß-D-glucopyranoside (13 mg), apigenin 6-C-ß-D-glucopyranosyl-8-C-α-L-arabinopyranoside (18 mg) and apigenin 6,8-di-C-ß-D-glucopyranoside (48 mg) were obtained in a one-step HSCCC separation from 240 mg of the sample. The purity of each compound was over 95% as determined by HPLC. Chemical structures of the isolated compounds were identified by electrospray ionization mass spectrometry (ESI-MS-MS) and nuclear magnetic resonance (NMR) methods. Moreover, the four compounds were isolated from LP for the first time.


Assuntos
Distribuição Contracorrente/métodos , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Nelumbo/química , Extratos Vegetais/química , Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Glicosídeos/análise , Espectroscopia de Ressonância Magnética , Caules de Planta/química , Espectrometria de Massas por Ionização por Electrospray
14.
J Sep Sci ; 41(2): 571-577, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29087605

RESUMO

An effective high-speed countercurrent chromatography method was successfully established by using ionic liquids as the modifier of the two-phase solvent system. Adding a small amount of ionic liquids significantly shortens the separation time and improves the separation efficiency. The conditions of ionic-liquid-modified high-speed countercurrent chromatography including solvent systems, types and content of added ionic liquids, and ionic liquids posttreatment were investigated. The established method was successfully applied to separate alkaloids from lotus leaves using a two-phase solvent system composed of petroleum ether/ethyl acetate/methanol/water/[C4 mim][BF4 ] (1:5:1:5:0.15, v/v/v/v/v). Four alkaloids pronuciferine (1.7 mg), N-nornuciferine (4.3 mg), nuciferine (3.1 mg), and roemerine (2.1 mg) were obtained with the purities of 90.53, 92.25, 99.86, and 98.63%, respectively, from 100 mg crude extract of lotus leaves. The results indicated that the ionic-liquid-modified high-speed countercurrent chromatography method was suitable for alkaloid separation from lotus leaves and would be a promising method for the separation of alkaloids from other natural products.


Assuntos
Alcaloides/isolamento & purificação , Nelumbo/química , Extratos Vegetais/química , Folhas de Planta/química , Aporfinas/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Distribuição Contracorrente , Líquidos Iônicos , Pós , Solventes
15.
J Cell Biochem ; 119(2): 1441-1452, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-28731223

RESUMO

Doxorubicin (DOX) induced cardiotoxicity is a major problem during chemotherapy of cancers. DOX-mediated suppression of type 1 IGF receptor (IGF-1R) signaling leads to cardiac dysfunction. Neferine, a bisbezylisoquinoline alkaloid from the seed embryos of Nelumbo nucifera Gaertn possesses a distinct range of pharmacological properties. Herewith, the present study attempts to elucidate the protective role of neferine against DOX induced toxicity in H9c2 rat cardiomyoblast cell line model. DOX-treated H9c2 cells significantly increased mitochondrial superoxide generation, depleted cellular antioxidant status, suppressed the activation of IGF-1R signaling via PI3K/Akt/mTOR and induced autophagy by the activation of ULK1, Beclin1, Atg7, and LC3B. Neferine pre-treatment activated IGF-1R signaling, improved cellular antioxidant pool, increased the expression of down-stream targets of IGF-1R, such as PI3K/Akt/mTOR, inhibited mitochondrial superoxide generation and autophagy significantly with the induction of Nrf2 translocation and expressions of HO1 and SOD1. Our study suggests the use of neferine for amelioration of DOX-mediated cardiotoxicity.


Assuntos
Benzilisoquinolinas/farmacologia , Doxorrubicina/efeitos adversos , Miócitos Cardíacos/citologia , Fator 2 Relacionado a NF-E2/metabolismo , Receptor IGF Tipo 1/metabolismo , Animais , Autofagia/efeitos dos fármacos , Linhagem Celular , Regulação da Expressão Gênica/efeitos dos fármacos , Heme Oxigenase-1/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Miócitos Cardíacos/efeitos dos fármacos , Miócitos Cardíacos/metabolismo , Nelumbo/química , Extratos Vegetais/química , Ratos , Transdução de Sinais/efeitos dos fármacos , Superóxido Dismutase-1/metabolismo
16.
J Diet Suppl ; 15(1): 11-23, 2018 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-28485986

RESUMO

Polyphenolic compounds have been suggested to be involved in the preservation of neural function via the production of neurotrophic factors in the brain. The nonedible joint part of lotus root (a rhizome of Nelumbo nucifera) has been reported to contain large amounts of polyphenolic compounds and, therefore, is expected to improve neural function by stimulating the production of brain-derived neurotrophic factor (BDNF) in glial cells. The effect of the aqueous extract prepared from the joint part of lotus root on BDNF gene expression was examined in C6 glioma cells as an in vitro model. This extract was shown to increase BDNF messenger ribonucleic acid (mRNA) levels to the elevation of HO-1 mRNA levels in the glioma cells, but failed to cause the elevation of BDNF mRNA levels in the cells pretreated with a HO-1 antisense oligodeoxynucleotide (ODN) or an HO-1 enzyme inhibitor zinc protoporphyrin (ZnPP). These findings strongly suggest that the aqueous extract prepared from the nonedible joint part of lotus root might be able to stimulate BDNF gene expression by enhancing HO-1 activity in the glioma cells, proposing the possibility that the joint part of lotus root might potentially improve neural function through the stimulation of BDNF production in glial cells.


Assuntos
Fator Neurotrófico Derivado do Encéfalo/efeitos dos fármacos , Heme Oxigenase-1/metabolismo , Nelumbo/química , Extratos Vegetais/farmacologia , Rizoma/química , Animais , Linhagem Celular Tumoral , Glioma/genética , Neuroglia/metabolismo , RNA Mensageiro/efeitos dos fármacos , Ratos
17.
Fitoterapia ; 124: 58-65, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29108933

RESUMO

A new skeleton benzylisoquinoline (BI) named neoliensinine (1) was isolated from embryos of lotus seed (Nelumbo nucifera Gaertn.), a traditional Chinese herb. The tribenzylisoquinoline (TBI) structure of 1 was confirmed by interpreting spectroscopic data of UV, IR, MS, 1D and 2D NMR. The stereo-configurations of the new compound, together with two known bisbenzylisoquinolines (BBI), neferine and isoliensinine were established by analyzing 1H NMR and 13C NMR spectra. The relaxation of 1, neferine, isoliensinine and liensinine in isolated mesenteric vascular smooth muscle (VSM) was evaluated. All the four BIs could efficiently inhibit MVSM contraction induced by 124mM KCl, with IC50 values of 2.407µM (1), 1.169µM (neferine), 3.504µM (isoliensinine) and 3.583µM (liensinine), respectively, suggesting that they were all potential relaxants for abnormal smooth muscle contractions. Interestingly, VSM treated by the three BBIs could re-contract when being stimulated by KCl after the drugs were removed, while VSM dealt with the TBI couldn't. It indicated that 1 has much high affinity with the molecular targets on relaxation of VSM contraction, which may relate to the unique skeleton with three BI groups.


Assuntos
Alcaloides/isolamento & purificação , Músculo Liso/efeitos dos fármacos , Nelumbo/química , Fármacos Neuromusculares/isolamento & purificação , Animais , Benzilisoquinolinas/isolamento & purificação , Feminino , Técnicas In Vitro , Isoquinolinas/isolamento & purificação , Masculino , Camundongos Endogâmicos C57BL , Estrutura Molecular , Fenóis/isolamento & purificação , Sementes/química
18.
Zhongguo Zhong Yao Za Zhi ; 42(8): 1525-1531, 2017 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-29071857

RESUMO

To investigate the overall intestinal permeability of multiple components in lotus leaves and make clear the interaction in composition absorption process. Rat single-pass intestinal perfusion technique was used, and the results showed that the Peff values of nuciferine, demethylanuciferine, rutin, quercetin, kaempferol from lotus leaf were greater than 0.5×10⁻4 cm•s⁻¹. In the biopharmaceutics classification system (BCS) intestinal permeability property, these ingredients were high permeable components, while the hyperin was low permeable component. However, in the multi-component environment of the lotus leaf extract, component permeation was changed. Semi quantitative analysis of the unclear components showed that under the multi-component environment, four in seven components with relatively high contents had a Peff value less than 0.5×10⁻4 cm•s⁻¹, indicating these 4 components were of low permeability, while other 3 components were of high permeability. The results could be valuable to make clear the overall intestinal permeability of multiple components in lotus leaf, and lay a foundation for studying the mechanism of the lipid-lowering effect of lotus leaf.


Assuntos
Absorção Intestinal , Intestinos/efeitos dos fármacos , Nelumbo/química , Extratos Vegetais/farmacocinética , Animais , Biofarmácia , Perfusão , Permeabilidade , Folhas de Planta/química , Ratos
19.
Carbohydr Polym ; 175: 721-727, 2017 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-28917922

RESUMO

The effects of lotus root amylopectin (LRA) on the formation of whey protein isolate (WPI) gels were investigated at the concentration range from 0 to 1.0% (w/v) by determining the texteral, thermal and rheological properties. Results exhibited these properties of WPI gels could be significantly enhanced by the addition of LRA in a concentration-dependent manner. Compared the gel with free of starch, the gel strength, water holding capacity and thermal transition temperature of WPI gel containing 1% (w/v) amylopectin were enhanced by 12.7-fold, 24.9% and 3.6°C, respectively. According to the analysis of scanning electron microscopy, colorimetric reaction and Fourier transform infrared spectroscopy measurements, it was concluded that the LRA-induced enhancement of WPI gel properties was possibly attributed to the formation of stable three-dimensional gel network, increased contents of reactive sulfhydryl group, CN bond and/or NH bond. Results suggested that LRA might be a good gel modifier.


Assuntos
Amilopectina/química , Géis , Nelumbo/química , Proteínas do Soro do Leite/química , Proteínas do Leite , Raízes de Plantas/química , Reologia , Amido
20.
Environ Toxicol ; 32(11): 2327-2340, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28804948

RESUMO

Lotus (Nelumbo nucifera Gaertn) possesses antioxidant, hepatoprotective, and anticancer potential. This study determined the protective role of aqueous extract from Nelumbo nucifera leaves (NLE) against N-diethylnitrosamine (DEN)-induced oxidative stress and hepatocellular carcinogenesis in a sample of Sprague-Dawley rats. NLE was fed orally to rats in which hepatic carcinoma was induced with DEN for 12 weeks. Five groups of 12 rats each were used for the study: Group I (control group) rats received distilled water; Group II rats were induced with DEN; Group III rats were induced with DEN and cotreated with 0.5% NLE; Group IV rats were induced with DEN and cotreated with 1.0% NLE; and Group V rats were induced with DEN and cotreated with 2.0% NLE. Clinical chemistry, organ weight, inflammatory marker, protein expression, enzyme, and antioxidant analyses were conducted. NLE administration to rats resulted in significantly decreased levels of serum alanine aminotransferase, aspartate aminotransferase, and albumin, which is indicative of hepatocellular damage, compared with the control group. DEN-induced oxidative stress was inhibited by NLE and this inhibition was paralleled by decreased lipid peroxides and increased glutathione transferase, superoxide dismutase, catalase, and glutathione peroxidase activity in liver tissues. The status of nonenzymatic antioxidants, such as reduced glutathione, was also found to be increased in NLE-administered rats. Furthermore, NLE decreased tumor size, hepatic Rac1, PKCα, and GSTπ expressions compared with the DEN-only group. Thus, supplementation of NLE reduced the adverse changes that occur because of liver cancer. These results prove that NLE protects against liver carcinogenesis induced because of treatment with DEN through blocking lipid peroxidation, hepatic cell damage, and enhancing the antioxidant defense system.


Assuntos
Antineoplásicos Fitogênicos/uso terapêutico , Carcinoma Hepatocelular/tratamento farmacológico , Dietilnitrosamina , Neoplasias Hepáticas/tratamento farmacológico , Nelumbo/química , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Lesões Pré-Cancerosas/tratamento farmacológico , Alanina Transaminase/metabolismo , Animais , Antioxidantes/metabolismo , Aspartato Aminotransferases/metabolismo , Carcinoma Hepatocelular/induzido quimicamente , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Catalase/metabolismo , Glutationa/metabolismo , Glutationa Transferase/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Neoplasias Hepáticas/induzido quimicamente , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Masculino , Lesões Pré-Cancerosas/induzido quimicamente , Lesões Pré-Cancerosas/metabolismo , Lesões Pré-Cancerosas/patologia , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismo
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