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1.
Mater Sci Eng C Mater Biol Appl ; 128: 112291, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34474842

RESUMO

Gold nanoclusters (AuNCs) have attracted much attention for tumor theranostics in recent years because of their ability of renal clearance and to escape the reticuloendothelial system (RES) sequestration. In this study, we presented a novel method to synthesize 68Ga-doped (labeled) AuNCs by simultaneous reduction of 68GaCl3 and HAuCl4 by glutathione. As synthesized 68Ga-doped, glutathione-coated AuNCs (68Ga-GSH@AuNCs) were ultrasmall in size (<2 nm), highly stable under physiological conditions and renally clearable, and had high efficiency for tumor targeting. To demonstrate the universality of this 68Ga labeling method and further enhance tumor targeting efficiency, arginine-glycine-aspartate (RGD)-containing peptide was introduced as co-reductant to synthesize RGD peptide and glutathione co-coated, 68Ga-labeled AuNCs (68Ga-RGD-GSH@AuNCs). Introduction of RGD peptide did not interfere the synthesis process but significantly enhanced the tumor targeting efficiency of the AuNCs. Our study demonstrated that it was feasible to label AuNCs with gallium-68 by direct reduction of the radioisotope and HAuCl4 with reductant peptides, holding a great potential for clinical translation for PET/CT detection of tumors.


Assuntos
Nanopartículas Metálicas , Neoplasias , Radioisótopos de Gálio , Glutationa , Ouro , Humanos , Neoplasias/diagnóstico por imagem , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada
2.
Mater Sci Eng C Mater Biol Appl ; 128: 112293, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34474844

RESUMO

Due to increased requirements for precision cancer treatment, cancer chemotherapy and combination therapies have gradually developed in the direction of diagnosis and treatment integration. In this study, a non-toxic nano carrier that demonstrates integrated MRI signal enhancing performance, as well as better chemotherapy and photothermal conversion performance, was prepared and characterized. Furthermore, the carrier was used to construct an integrated system of tumor diagnosis and treatment. Our in vitro studies showed that this system has a considerable inhibition effect on tumor cells during the treatment of chemotherapy when combined with PTT, and in vivo studies showed that the system could improve the MRI signal of the tumor site with application of a safe dosage. Thus, this system based on NGO/USPIO has the potential to be a multi-functional nano drug delivery system integrating diagnosis and treatment benefits and applications that are worthy of further research.


Assuntos
Grafite , Nanopartículas de Magnetita , Neoplasias , Dextranos , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Óxidos
3.
Nanoscale ; 13(33): 14245-14253, 2021 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-34477707

RESUMO

The design of multifunctional nanoplatforms is of great importance for improving hypoxia-induced therapeutic outcomes, especially for overcoming radiotherapy (RT) tolerance. Here, two-dimensional intermetallic PtBi/Pt nanoplates (PtBi NPs) were designed as a therapeutic platform to in situ generate oxygen, and thereby overcome tumor hypoxia for boosting photothermal/radiotherapy (PTT/RT). With high X-ray attenuation coefficient, PtBi NPs exhibited outstanding radiotherapy sensitization characteristics. Moreover, the high photothermal effect of PtBi NPs could promote the catalytic activity of PtBi NPs to achieve a synergistic PTT/RT effect. PEGylated PtBi NPs (PtBi-PEG) exhibited excellent biocompatibility, prolonged blood circulation time and enhanced tumor accumulation. Finally, PtBi-PEG showed excellent trimodal contrast enhancement for infrared (IR) imaging, photoacoustic (PA) imaging and X-ray imaging, facilitating imaging-guided cancer therapy. Thus, our work highlights PtBi-PEG as a novel multifunctional theranostic nanoplatform with great potential for future multimodal imaging-guided synergistic cancer therapy.


Assuntos
Neoplasias , Técnicas Fotoacústicas , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fototerapia , Nanomedicina Teranóstica , Hipóxia Tumoral
4.
Nanoscale ; 13(31): 13410-13420, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34477746

RESUMO

Photoacoustic imaging (PAI)-guided photothermal therapy (PTT) has drawn considerable attention due to the deeper tissue penetration and higher maximum permissible exposure. However, current phototheranostic agents are greatly restricted by weak absorption in the second near-infrared (NIR-II, 1000-1700 nm) window, long-term toxicity, and poor photostability. In this report, novel organic NIR-II conjugated polymer nanoparticles (CPNs) based on narrow bandgap donor-acceptor BDT-TBZ polymers were developed for effective cancer PAI and PTT. Characterization data confirmed the high photothermal conversion efficiency, good photostability, excellent PAI performance, and superior biocompatibility of as-obtained CPNs. In addition, in vitro and in vivo tests demonstrated the efficient PTT effect of CPNs in ablating cancer cells and inhibiting tumor growth under 1064 nm laser irradiation. More importantly, the CPNs exhibited rapid clearance capability through the biliary pathway and negligible systematic toxicity. Thus, this work provides a novel organic theranostic nanoplatform for NIR-II PAI-guided PTT, which advances the future clinical translation of biocompatible and metabolizable conjugated nanomaterials in cancer diagnosis and therapy.


Assuntos
Nanopartículas , Neoplasias , Técnicas Fotoacústicas , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fototerapia , Polímeros , Medicina de Precisão , Nanomedicina Teranóstica
5.
Rofo ; 193(S 02): S74-S76, 2021 Sep.
Artigo em Alemão | MEDLINE | ID: mdl-34470076
6.
Nano Lett ; 21(16): 6914-6922, 2021 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-34428906

RESUMO

The highly up-regulated glutathione (GSH) concentration in the tumor microenvironment is generally identified to be an effective endogenous characteristic of cancerous tissues. Herein, an ultrahigh-sensitive and tumor-specific photoacoustography technique in the near-infrared (NIR-II) region based on optical writing and redox-responsive chromogenic graphic fixing is developed by introducing a self-synthesized photosensitive silver bromide modified with poly lactic-co-glycolic acid (AgBr@PLGA) nanocrystals. After they are optically triggered by external light, the NIR-transparent AgBr@PLGA nanocrystals can be reduced by the tumor-abundant GSH into strongly absorbing silver nanoparticles, significantly boosting the "turn-on" photoacoustic (PA) signal in the NIR-II region; therefore, the tumor area can be graphically fixed and developed in the photoacoustography. Experiments on both in vitro phantoms and in vivo mouse models demonstrate that the tumor area is specifically identified by the photoacoustography with the background signals effectively suppressed by dynamically modulating the exposure time. The tumor-specific photoacoustography technique prefigures great potential for high-precision cancer diagnosis and treatment monitoring.


Assuntos
Nanopartículas Metálicas , Neoplasias , Animais , Camundongos , Neoplasias/diagnóstico por imagem , Oxirredução , Prata , Microambiente Tumoral , Redação
7.
Anal Chem ; 93(32): 11275-11283, 2021 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-34342424

RESUMO

Accurate diagnosis and targeted therapy are essential to precision theranostics. However, nonspecific response of theranostic agents in healthy tissues impedes their practical applications. Here, we design an activatable DNA nanosphere for specifically in situ sensing of cancer biomarker flap endonuclease 1 (FEN1) and spatiotemporally modulating drug release. The gold nanostar-conjugated FEN1 substrate acts as spherical nucleic acid and induces a fluorescence signal upon a FEN1 stimulus for diagnosis. Guided by the nanoflare, external NIR light then triggers a controlled release of carried drugs at desired sites. This DNA nanosphere not only exhibits good stability, sensitivity, and specificity toward FEN1 assay but also serves as a precision theranostic agent for targeted and controlled drug delivery. Our study provides a reliable method for FEN1 imaging in vitro and in vivo and suggests a powerful strategy for precision medicine.


Assuntos
Neoplasias , Ácidos Nucleicos , Preparações Farmacêuticas , Sistemas de Liberação de Medicamentos , Endonucleases Flap , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico
8.
Int J Mol Sci ; 22(15)2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34360822

RESUMO

Brillouin spectroscopy has recently gained considerable interest within the biomedical field as an innovative tool to study mechanical properties in biology. The Brillouin effect is based on the inelastic scattering of photons caused by their interaction with thermodynamically driven acoustic modes or phonons and it is highly dependent on the material's elasticity. Therefore, Brillouin is a contactless, label-free optic approach to elastic and viscoelastic analysis that has enabled unprecedented analysis of ex vivo and in vivo mechanical behavior of several tissues with a micrometric resolution, paving the way to a promising future in clinical diagnosis. Here, we comprehensively review the different studies of this fast-moving field that have been performed up to date to provide a quick guide of the current literature. In addition, we offer a general view of Brillouin's biomedical potential to encourage its further development to reach its implementation as a feasible, cost-effective pathology diagnostic tool.


Assuntos
Espalhamento de Radiação , Análise Espectral/métodos , Animais , Doenças Ósseas/diagnóstico por imagem , Humanos , Neoplasias/diagnóstico por imagem
9.
Anal Chem ; 93(34): 11751-11757, 2021 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-34398599

RESUMO

Developing nanoplatforms that simultaneously integrate diagnostic imaging and therapy functions has been a promising but challenging task for cancer theranostics. Herein, we report the rational design of a smart nucleic acid-gated covalent organic framework (COF) nanosystem for cancer-specific imaging and microenvironment-responsive drug release. Cy5 dye-labeled single-stranded DNA (ssDNA) for mRNA recognition was adsorbed on the surface of doxorubicin (Dox)-loaded COF nanoparticles (NPs). Dox loaded in the pores of COF NPs could strengthen the interactions between ssDNA and COF and enhance the fluorescence quenching effect toward Cy5, while the densely coated ssDNA could prevent the leakage of Dox from COF NPs. The obtained nanosystem exhibited low fluorescence signal and Dox release in normal cells; however, the ssDNA could be released by the overexpressed TK1 mRNA in cancer cells to recover the intense fluorescence signal of Cy5, and the loaded Dox could be further released for chemotherapy. Therefore, cancer cell-specific diagnostic imaging and drug release were realized with the rationally developed nanosystem. This work offers a universal nanoplatform for cancer theranostics and a promising strategy for regulating the interaction between COFs and biomolecules.


Assuntos
Estruturas Metalorgânicas , Nanopartículas , Neoplasias , Ácidos Nucleicos , Diagnóstico por Imagem , Doxorrubicina/farmacologia , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico
11.
Radiol Clin North Am ; 59(5): 875-886, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34392924

RESUMO

Fluorodeoxyglucose (FDG) PET/CT is sensitive to metabolic, immune-related, and structural changes that can occur in tumors in cancer immunotherapy. Unique mechanisms of immune checkpoint inhibitors (ICIs) occasionally make response evaluation challenging, because tumors and inflammatory changes are both FDG avid. These response patterns and sequelae of ICI immunotherapy, such as immune-related adverse events, are discussed. Immune-specific PET imaging probes at preclinical stage or in early clinical trials, which may help guide clinical management of cancer patients treated with immunotherapy and likely have applications outside of oncology for other diseases in which the immune system plays a role, are reviewed.


Assuntos
Imunoterapia/tendências , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Tomografia por Emissão de Pósitrons combinada à Tomografia Computadorizada/tendências , Fluordesoxiglucose F18 , Humanos , Compostos Radiofarmacêuticos
12.
Methods Enzymol ; 657: 111-144, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34353484

RESUMO

In this chapter, we discuss the need for the development of enzyme-activatable probes in the field of tumor-targeted photoacoustic (PA) imaging, then we give a brief description of the innovation of designing alkaline phosphatase (ALP)-activatable probes for PA imaging. After that, we provide detailed protocols for the syntheses and characterizations of a near-infrared photoacoustic imaging probe, 1P, developed in our research group. With this tool, 1P could form nanoparticles 1-NPs under the catalysis of ALP and thus could be used to enhance PA imaging both in vitro and in vivo.


Assuntos
Nanopartículas , Neoplasias , Técnicas Fotoacústicas , Fosfatase Alcalina , Corantes , Humanos , Neoplasias/diagnóstico por imagem
13.
Methods Enzymol ; 657: 21-57, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34353488

RESUMO

Photoacoustic (PA) imaging is an emerging imaging technique, which combines high spatial resolution and deep tissue penetration of ultrasound imaging with high sensitivity of fluorescence imaging. In the past few years, PA has shown promise for noninvasive imaging of biomolecules in vivo. In this chapter, we present the synthesis and application of a tumor targeting and caspase-3 activatable PA probe (1-RGD) for real-time and noninvasive imaging of tumor apoptosis. 1-RGD can be efficiently delivered into tumor tissues and recognized by caspase-3, which triggered efficient proteolysis of DEVD substrate and subsequent intramolecular macrocyclization, followed by in situ self-assembly into nanoparticles, leading to prolonged retention in apoptotic tumors and enhanced PA signals. With 1-RGD, high-resolution 3D PA images of tumor tissues can be obtained, allowing to report on the activity and distribution of caspase-3 within DOX-treated tumors, which was helpful for early monitoring of tumor response to therapy. We provide detailed protocols for the synthesis, in vitro characterization and in vivo applications of 1-RGD.


Assuntos
Neoplasias , Técnicas Fotoacústicas , Apoptose , Caspase 3 , Humanos , Imagem Molecular , Neoplasias/diagnóstico por imagem
14.
Methods Enzymol ; 657: 89-109, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34353500

RESUMO

Matrix metalloproteinase-2 (MMP-2), which is one of MMPs family, is known as an extracellular gelatinase controlling cancer cell adhesion, growth, and metastasis. Because of the great interest in MMP-2 activity, the detailed protocols for evaluating MMP-2-responsive contrast agents, especially photoacoustic probes for in vivo use, are helpful for researchers in the field. We here describe the detailed synthetic procedure of MMP-2-activatable photoacoustic probe AlNc-pep-PEG consisting of aluminum naphthalocyanine, MMP-2-responsive peptide sequence, and poly(ethylene glycol), which has recently been developed in our research group. The detailed measurement protocol of photoacoustic signal intensity in vitro and in vivo by using in-house built photoacoustic signal measurement system and photoacoustic imaging apparatus are also summarized.


Assuntos
Metaloproteinase 2 da Matriz , Neoplasias , Alumínio , Meios de Contraste , Humanos , Imagem Molecular , Neoplasias/diagnóstico por imagem
15.
ACS Appl Mater Interfaces ; 13(33): 39112-39125, 2021 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-34384220

RESUMO

Autophagy inhibition could hinder the underlying protective mechanisms in the course of tumor treatment. The advances in autophagy inhibition have driven focus on the functionalized nanoplatforms by combining the current treatment paradigms with complementary autophagy inhibition for enhanced efficacy. Furthermore, Ca2+ overload is also a promising adjuvant target for the tumor treatment by augmenting mitochondrial damage. In this view, complementary mitochondrial Ca2+ overload and autophagy inhibition were first demonstrated as a novel strategy suitable for homing in on the shortage of photodynamic therapy (PDT). We constructed biodegradable tumor-targeted inorganic/organic hybrid nanocomposites (DPGC/OI) synchronously encapsulating IR780 and Obatoclax by biomineralization of the nanofilm method, which consists of pH-triggered calcium phosphate (CP), long circulation phospholipid block copolymers 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE)-poly(ethylene glycol) (PEG)2000-glucose (DPG). In the presence of the hydrophilic PEG chain and glucose transporter 1 (Glut-1) ligands, DPGC would become an effectively tumor-oriented nanoplatform. Subsequently, IR780 as an outstanding photosensitizer could produce increased amounts of toxic reactive oxygen species (ROS) after laser irradiation. Calcium phosphate (CP) as the Ca2+ nanogenerator could generate Ca2+ at low pH to induce mitochondrial Ca2+ overload. The dysfunction of mitochondria could enhance increased amounts of ROS. Based on the premise that autophagy would degrade dysfunctional organelles to sustain metabolism and homeostasis, which might participate in resistance to PDT, Obatoclax as an autophagy inhibitor would hinder the protective mechanism from cancer cells with negligible toxicity. Such an enhanced PDT via mitochondrial Ca2+ overload and autophagy inhibition could be realized by DPGC/OI.


Assuntos
Autofagia/efeitos dos fármacos , Fosfatos de Cálcio/química , Glucose/química , Indóis/química , Nanocompostos/química , Fosfatidiletanolaminas/química , Fármacos Fotossensibilizantes/química , Polietilenoglicóis/química , Animais , Transporte Biológico , Melhoramento Biomédico , Feminino , Humanos , Indóis/metabolismo , Indóis/farmacologia , Camundongos Endogâmicos BALB C , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Fosfolipídeos/química , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Pirróis/química , Pirróis/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais , Propriedades de Superfície , Distribuição Tecidual
16.
Int J Mol Sci ; 22(15)2021 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-34361080

RESUMO

Photoimmunotherapy (PIT) is an upcoming potential cancer treatment modality, the effect of which is improved in combination with chemotherapy. PIT causes a super-enhanced permeability and retention (SUPR) effect. Here, we quantitatively evaluated the SUPR effect using radiolabeled drugs of varying molecular weights (18F-5FU, 111In-DTPA, 99mTc-HSA-D, and 111In-IgG) to determine the appropriate drug size. PIT was conducted with an indocyanine green-labeled anti-HER2 antibody and an 808 nm laser irradiation. Mice were subcutaneously inoculated with HER2-positive cells in both hindlimbs. The tumor on one side was treated with PIT, and the contralateral side was not treated. The differences between tumor accumulations were evaluated using positron emission tomography or single-photon emission computed tomography. Imaging studies found increased tumor accumulation of agents after PIT. PIT-treated tumors showed significantly increased uptake of 18F-5FU (p < 0.001) and 99mTc-HSA-D (p < 0.001). A tendency toward increased accumulation of 111In-DTPA and 111In-IgG was observed. These findings suggest that some low- and medium-molecular-weight agents are promising candidates for combined PIT, as are macromolecules; hence, administration after PIT could enhance their efficacy. Our findings encourage further preclinical and clinical studies to develop a combination therapy of PIT with conventional anticancer drugs.


Assuntos
Anticorpos Monoclonais/administração & dosagem , Sistemas de Liberação de Medicamentos , Imunoterapia/métodos , Neoplasias/terapia , Fototerapia/métodos , Cintilografia/métodos , Animais , Apoptose , Proliferação de Células , Terapia Combinada , Humanos , Verde de Indocianina/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Neoplasias/diagnóstico por imagem , Neoplasias/metabolismo , Neoplasias/patologia , Células Tumorais Cultivadas , Ensaios Antitumorais Modelo de Xenoenxerto
17.
Methods Mol Biol ; 2350: 105-123, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34331282

RESUMO

Early detection of malignant tumors, micrometastases, and disseminated tumor cells is one of the effective way of fighting cancer. Among the many existing imaging methods like computed tomography (CT), ultrasound (US), magnetic resonance imaging (MRI), positron emission tomography (PET), and single-photon emission computed tomography (SPECT), optical imaging with fluorescent probes is one of the most promising alternatives because it is fast, inexpensive, safe, sensitive, and specific. However, traditional fluorescent probes, based on organic fluorescent dyes, suffer from the low signal-to-noise ratio. Furthermore, conventional organic fluorescent dyes are unsuitable for deep tissue imaging because of the strong visible light absorption by biological tissues. The use of fluorescent semiconductor nanocrystals, or quantum dots (QDs), may overcome this limitation due to their large multiphoton cross section, which ensures efficient imaging of thick tissue sections inaccessible with conventional fluorescent probes. Moreover, the lower photobleaching and higher brightness of fluorescence signals from QDs ensures a much better discrimination of positive signals from the background. The use of fluorescent nanoprobes based on QDs conjugated to uniformly oriented high-affinity single-domain antibodies (sdAbs) may significantly increase the sensitivity and specificity due to better recognition of analytes and deeper penetration into tissues due to small size of such nanoprobes.Here, we describe a protocol for the fabrication of nanoprobes based on sdAbs and QDs, preparation of experimental xenograft mouse models for quality control, and multiphoton imaging of deep-tissue solid tumors, micrometastases, and disseminated tumor cells.


Assuntos
Imunofluorescência/métodos , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Neoplasias/diagnóstico por imagem , Neoplasias/patologia , Pontos Quânticos , Anticorpos de Domínio Único , Linhagem Celular Tumoral , Imunofluorescência/normas , Humanos , Imunoconjugados/química , Imuno-Histoquímica/métodos , Sondas Moleculares , Imagem Multimodal/métodos , Nanopartículas , Micrometástase de Neoplasia , Imagem Óptica/métodos
18.
J Photochem Photobiol B ; 221: 112257, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34271410

RESUMO

Organic semiconductor small molecules IHIC and ITIC have been developed as solar cell materials, and because of their strong near-infrared absorption capabilities, they are promising for cancer phototherapy. This article reports the application of semiconductor small molecule IHIC/ITIC liposomes in photothermal therapy and photoacoustic imaging of tumors firstly. Experiments show that the liposome-loaded IHIC/ITIC material has good biocompatibility and can be effectively enriched in tumor sites. After being irradiated with laser, it can emit strong photoacoustic signals, and has achieved good results in the photothermal treatment of breast cancer mice. We believe that organic semiconductor small molecule IHIC/ITIC will become a promising photothermal agent with wonderful development possibilities.


Assuntos
Materiais Biocompatíveis/química , Neoplasias/tratamento farmacológico , Fármacos Fotossensibilizantes/uso terapêutico , Semicondutores , Animais , Materiais Biocompatíveis/metabolismo , Materiais Biocompatíveis/farmacologia , Materiais Biocompatíveis/uso terapêutico , Sobrevivência Celular/efeitos dos fármacos , Endocitose/efeitos dos fármacos , Endocitose/efeitos da radiação , Células HeLa , Humanos , Lasers , Lipossomos/química , Camundongos , Camundongos Nus , Microscopia Confocal , Neoplasias/diagnóstico por imagem , Neoplasias/patologia , Tamanho da Partícula , Técnicas Fotoacústicas , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/metabolismo , Fármacos Fotossensibilizantes/farmacologia , Terapia Fototérmica , Transplante Heterólogo
19.
Int J Pharm ; 606: 120905, 2021 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-34293466

RESUMO

Cancer is one of the most prevalent and deadly diseases in the world, to which conventional treatment options, such as chemotherapy and radiotherapy, have been applied to overcome the disease or used in a palliative manner to enhance the quality of life of the patient. However, there is an urgent need to develop new preventive and treatment strategies to overcome the limitations of the commonly used approaches. The field of cancer nanomedicine, and more recently the field of nanotheranostics, where imaging and therapeutic agents are combined in a single platform, provide new opportunities for the treatment and the diagnosis of cancer. This combination could bring us closer to a more personalized and cared-for therapy, in opposition to the conventional and standardized approaches. Gene therapy is a promising strategy for the treatment of cancer that requires a transport system to efficiently deliver the genetic material into the target cells. Hence, the main purpose of this work was to review recent findings and developments regarding theranostic nanosystems that incorporate both gene therapy and imaging agents for cancer treatment.


Assuntos
Nanopartículas , Neoplasias , Terapia Genética , Humanos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Medicina de Precisão , Qualidade de Vida , Nanomedicina Teranóstica
20.
Colloids Surf B Biointerfaces ; 206: 111966, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34293577

RESUMO

The release and biodistribution of drugs in the body have an important impact on tumor diagnosis and treatment. Near-infrared (NIR) fluorescent active fluorophores with good photostability are used to detect drug release and perform in vivo imaging. Here, we developed a glutathione-responsive NIR prodrug POEGMA-b-P(CPT-CyOH) (PCC) for effective cancer diagnosis and treatment, whereby the camptothecin (CPT) and NIR fluorophore CyOH in PCC are connected by disulfide bonds. In vitro experiments confirmed that PCC was quickly taken up by cells. The high concentration of tumor intracellular glutathione caused the cleavage of the PCC disulfide bonds, leading to the release of the chemotherapeutic drug CPT, indicating that PCC can promote apoptosis. Moreover, owing to the fluorescent properties of CyOH, PCC was successfully used for in vivo imaging to observe the drug penetration and enrichment capabilities in tumors. Finally, PCC successfully inhibited tumor growth, indicating that the prodrug has a good anti-tumor effect. This work provides new strategies for chemical drug delivery and precise cancer treatment.


Assuntos
Nanopartículas , Neoplasias , Pró-Fármacos , Animais , Camptotecina/farmacologia , Linhagem Celular Tumoral , Feminino , Citometria de Fluxo , Glutationa/metabolismo , Células HeLa , Humanos , Camundongos Endogâmicos BALB C , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Imagem Óptica , Pró-Fármacos/farmacologia , Distribuição Tecidual
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