Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.416
Filtrar
1.
Dis Aquat Organ ; 141: 195-224, 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33150869

RESUMO

Recent surveys of white perch Morone americana from Chesapeake Bay, USA, revealed a high prevalence of hepatic and biliary lesions, including neoplasia, and bile duct parasites. Here, we describe lesions in the liver and gallbladder and evaluate for statistical associations among lesions, parasites, and biomarkers of chemical exposure in fish from 2 tributaries of Chesapeake Bay. Fish were collected from an estuarine site in the Choptank River (n = 122, ages 3-11), a tributary with extensive agriculture within the watershed, and the Severn River (n = 131, ages 2-16), a tributary with extensive urban development. Passive integrative samplers were deployed at the fish collection site and an upstream, non-tidal site in each river for 30 d. Intrahepatic biliary lesions observed in fish from both rivers included neoplasia (23.3%), dysplasia (16.2%), hyperplasia (46.6%), cholangitis (24.9%), and dilated ducts containing plasmodia of Myxidium sp. (24.9%). Hepatocellular lesions included foci of hepatocellular alteration (FHA, 15.8%) and neoplasia in 4 Severn River fish (2.3%). Age of fish and Myxidium sp. infections were significant risk factors for proliferative and neoplastic biliary lesions, age alone was a risk factor for FHA, and Goussia bayae infections were associated with cholangitis and cholecystitis. Lesion prevalence was higher in fish from the Severn River, which contained higher concentrations of PAHs, organochlorine pesticides, and brominated diphenyl ethers. Metabolite biomarkers indicated higher PAH exposures in Severn River fish. This study suggests Myxidium sp. as a promoter of bile duct tumors, but more data are needed to evaluate the biological effects of environmental contaminants in this species.


Assuntos
Bass , Doenças dos Peixes , Neoplasias , Parasitos , Poluentes Químicos da Água , Animais , Ductos Biliares , Monitoramento Ambiental , Doenças dos Peixes/epidemiologia , Neoplasias/veterinária , Prevalência
2.
Am J Vet Res ; 81(12): 940-949, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33251842

RESUMO

OBJECTIVE: To evaluate the effect of kernel and window settings on the assessment of small and complicated vasculature in CT angiographic (CTA) images of kidneys, jejunum with mesentery, and tumors in dogs. ANIMALS: 20 healthy dogs and 20 dogs with tumors. PROCEDURES: Images from CTA performed previously in dogs were reconstructed with 3 different combinations of kernel and window settings (soft kernel with soft tissue window, soft kernel with bone window, and sharp kernel with bone window), and reconstructed images of the left kidney and the jejunum with the mesentery in healthy dogs and tumors in affected dogs were evaluated by reviewers blinded to the settings. RESULTS: For images of kidney and jejunum with mesentery, reviewers' scores for the conspicuity of vascularity in the arterial phase and the differentiation of the organs from the adjacent structures were significantly higher when viewed in bone window (vs soft tissue window) regardless of kernel setting. For images of head and gastrointestinal tumors, reviewers' scores for differentiation of intratumoral vasculature were higher when viewed in sharp kernel with bone window versus other setting combinations. However, the conspicuity of gastrointestinal, hepatic, or splenic tumoral vessels from the adjacent structures had higher reviewer scores for images in soft kernel with soft tissue window, compared with other setting combinations. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that reconstruction of CTA images with sharp kernel combined with bone window settings might have clinical utility in evaluating and planning treatments for dogs with various tumors; however, additional research is warranted to further identify effects of various kernel and window setting combinations on assessments of small and complicated vasculature in larger and more diverse populations of dogs with and without tumors.


Assuntos
Doenças do Cão , Neoplasias , Angiografia , Animais , Angiografia por Tomografia Computadorizada/veterinária , Doenças do Cão/diagnóstico por imagem , Cães , Cabeça , Neoplasias/diagnóstico por imagem , Neoplasias/veterinária , Tomografia Computadorizada por Raios X/veterinária
3.
Arch Virol ; 165(11): 2409-2417, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32770481

RESUMO

Koala retrovirus (KoRV) is a major threat to koala health and conservation. It also represents a series of challenges across the fields of virology, immunology, and epidemiology that are of great potential interest to any researcher in the field of retroviral diseases. KoRV is a gammaretrovirus that is present in both endogenous and exogenous forms in koala populations, with a still-active endogenization process. KoRV may induce immunosuppression and neoplastic conditions such as lymphoma and leukemia and play a role in chlamydiosis and other diseases in koalas. KoRV transmission modes, pathogenesis, and host immune response still remain unclear, and a clear understanding of these areas is critical for devising effective preventative and therapeutic strategies. Research on KoRV is clearly critical for koala conservation. In this review, we provide an overview of the current understanding and future challenges related to KoRV epidemiology, transmission mode, pathogenesis, and host immune response and discuss prospects for therapeutic and preventive vaccines.


Assuntos
Gammaretrovirus/classificação , Transmissão Vertical de Doença Infecciosa , Phascolarctidae/virologia , Infecções por Retroviridae/veterinária , Sequência de Aminoácidos , Animais , Austrália/epidemiologia , Infecções por Chlamydia/veterinária , Infecções por Chlamydia/virologia , Evolução Molecular , Neoplasias/veterinária , Neoplasias/virologia , Phascolarctidae/imunologia , Infecções por Retroviridae/epidemiologia , Infecções por Retroviridae/transmissão
4.
Pesqui. vet. bras ; 40(6): 451-465, June 2020. tab, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1135646

RESUMO

Icterus (jaundice) is a yellowish pigmentation resulting from the depositing of bilirubin in tissues due to its high plasmatic concentration. The pathogenesis of icterus includes metabolic changes or obstructed bilirubin excretion and it is classified as pre-hepatic, hepatic and post-hepatic. This study aimed to evaluate and classify different causes of icterus in dogs during post mortem examination. These dogs were examined from 2014 to 2017, using macroscopic and histologic exams as well as ancillary tests. Eighty-three dogs were examined macroscopically and microscopically. They were separated into groups of icterus types: 24 (28.9%) dogs had pre-hepatic icterus, 45 (54.2%) had hepatic, 13 (15.7%) pre-hepatic and hepatic and one (1.2%) had post-hepatic icterus. Many factors were identified as a cause of icterus, including infectious agents (51/83), neoplasms (13/83), hepatic degeneration (11/83), chronic hepatic diseases (6/83), and obstructive causes (1/87). Among the infectious causes, leptospirosis, ehrlichiosis and disorders suggestive of septicemia were diagnosed. Neoplasms associated with icterus were cholangiocarcinoma, hemangiosarcoma and lymphoma. Other causes of icterus included degenerative diseases, such as lipidosis and glycogen degeneration. Hepatic fibrosis (cirrhosis) as a chronic disease and cholelithiasis also produced icterus. PCR was performed to confirm leptospirosis and ehrlichiosis. Samples of total DNA were used to amplify a fragment of a gene from Leptospira interrogans and Ehrlichia canis. In some dogs, co-infection of these agents was detected. The classification and identification of icterus etiologies in dogs is very important due to the number of diseases with this alteration, where ante mortem diagnosis is not always easily performed when some of these conditions are present.(AU)


Icterícia é a pigmentação amarelada decorrente da deposição de bilirrubina em tecidos devido à elevada concentração plasmática. A patogênese da icterícia inclui alterações no metabolismo ou na excreção de bilirrubina, sendo classificada em pré-hepática, hepática ou pós-hepática. O objetivo desse estudo foi identificar, avaliar e classificar as causas de icterícia em cães necropsiados de 2014 a 2017, associando as lesões macroscópicas, histológicas e exames complementares. Foram avaliados macro- e microscopicamente 83 cães com diferentes intensidades de icterícia. Os cães foram separados em grupos de acordo com o tipo de icterícia: 24 (28,9%) cães com icterícia pré-hepática, 45 (54,2%) cães com icterícia hepática, 13 (15,7%) com icterícia pré-hepática e hepática e um (1,2%) com icterícia pós-hepática. Foram identificadas várias etiologias associadas à icterícia, dentre elas pode-se destacar, agentes infecciosos (51/83), neoplasmas (13/83), processos degenerativos (11/83), crônicos (6/83) e obstrutivos (1/83). Dentre as causas infecciosas, destacam-se a leptospirose, a erliquiose e as lesões sugestivas de septicemia. Entre os neoplasmas associados com icterícia destacaram-se o colangiocarcinoma, hemangiossarcoma e linfoma. Outras causas de icterícia incluiriam os processos degenerativos como as degenerações gordurosa e glicogênica. Fibrose hepática (cirrose) e colelitíase foram também diagnosticados como causa de icterícia. A PCR foi utilizada para o diagnóstico confirmatório de leptospirose e erliquiose. Amostras de DNA total foram utilizadas para amplificar um fragmento dos genes de Leptospira interrogans e de Ehrlichia canis. Em alguns cães foi detectada co-infecção por estes agentes. A classificação e a identificação das causas de icterícia em cães são relevantes devido ao grande número de doenças que apresentam essa alteração, muitas vezes sem diagnóstico ante mortem.(AU)


Assuntos
Animais , Cães , Doenças do Cão , Icterícia/diagnóstico , Icterícia/etiologia , Icterícia/patologia , Icterícia/sangue , Icterícia/veterinária , Reação em Cadeia da Polimerase/veterinária , Ehrlichiose/veterinária , Sepse/veterinária , Diagnóstico Diferencial , Leptospirose/veterinária , Neoplasias/veterinária
5.
Vet Radiol Ultrasound ; 61(4): 385-393, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32400045

RESUMO

The ability to differentiate thoracic masses of mediastinal and pulmonary origins is often confounded by their complex spatial relationship. The objectives of this retrospective, observational cross-sectional study were to assess radiographic differentiation of mediastinal versus pulmonary masses, and to determine if there are any correlations with specific radiographic findings. Thoracic radiographs of 75 dogs and cats with mediastinal and/or pulmonary masses identified on CT were reviewed. Radiographic studies were anonymized, randomized, and reviewed twice by three reviewers. Reviewers categorized the origin of each mass(es) as mediastinal, pulmonary, or both. On the second review, the presence or absence of 21 different radiographic findings was recorded for each mass. Agreement between the radiographic and CT categorization of mass origin, as well as inter- and intraobserver agreement, was calculated. Overall agreement between radiographs and CT was moderate for both mediastinal (68.6%) and pulmonary masses (63%). Overall, interobserver agreement was moderate (κ = 0.50-0.74), with moderate to strong intraobserver agreement (κ = 0.58-0.93). Masses within the mediastinum were significantly more likely to displace other mediastinal structures. Alternatively, masses lateral to midline and in the caudal thorax were found to be significantly positively correlated with a pulmonary origin. The results of this study highlight the limitations of radiography for differentiation of mediastinal and pulmonary masses, with mass location and displacement of other mediastinal structures potentially useful for radiographic findings that may help improve accuracy.


Assuntos
Doenças do Gato/diagnóstico por imagem , Doenças do Cão/diagnóstico por imagem , Pneumopatias/veterinária , Doenças do Mediastino/veterinária , Neoplasias/veterinária , Animais , Gatos , Estudos Transversais , Cães , Feminino , Humanos , Pneumopatias/diagnóstico por imagem , Doenças do Mediastino/diagnóstico por imagem , Mediastino , Neoplasias/diagnóstico por imagem , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/veterinária
6.
Vet Radiol Ultrasound ; 61(4): 427-434, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32162400

RESUMO

In humans, contrast-enhanced CT (CECT) has been used to indirectly assess the antiangiogenic effects demonstrated by a number of tyrosine kinase inhibitors. This retrospective, cross-sectional study aimed to quantitatively evaluate changes in tumor contrast-enhancement (CE) using CECT in solid tumor-bearing dogs treated with toceranib phosphate (TOC). The changes in tumor size and CE were measured using the Hounsfield unit (HU) scale in CECT images before TOC treatment and between 30 and 90 days after initiating the treatment. Among the 36 dogs treated with TOC, eight (22.2%) showed a partial response, 22 (61.1%) showed stable disease, and six (16.7%) showed progressive disease. Thirty (83.3%) of 36 dogs showed a decrease in tumor CE (median: -20%, range: -1% to -48%) after initiating the treatment. The results indicated that tumor CE and size changes were observed in tumor-bearing dogs that were treated with TOC; however, tumor CE was not significantly correlated with tumor regression. We suggest that these results could serve as pilot data to evaluate the antiangiogenic effects associated with TOC.


Assuntos
Doenças do Cão/tratamento farmacológico , Indóis/uso terapêutico , Neoplasias/veterinária , Pirróis/uso terapêutico , Tomografia Computadorizada por Raios X/veterinária , Animais , Antineoplásicos/uso terapêutico , Meios de Contraste/farmacologia , Estudos Transversais , Doenças do Cão/diagnóstico por imagem , Cães , Feminino , Humanos , Masculino , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos
8.
Sci Total Environ ; 714: 135793, 2020 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-32018940

RESUMO

Recent studies suggest that oncogenic processes (from precancerous lesions to metastatic cancers) are widespread in wild animal species, but their importance for ecosystem functioning is still underestimated by evolutionary biologists and animal ecologists. Similar to what has been observed in humans, environmental modifications that often place wild organisms into an evolutionary trap and/or exposes them to a cocktail of mutagenic and carcinogenic pollutants might favor cancer emergence and progression, if animals do not up-regulate their defenses against these pathologies. Here, we compared, for the first time, the expression of 59 tumor-suppressor genes in blood and liver tissues of urban and rural great tits (Parus major); urban conditions being known to favor cancer progression due to, among other things, exposure to chemical or light pollution. Contrary to earlier indications, once we aligned the transcriptome to the great tit genome, we found negligible differences in the expression of anti-cancer defenses between urban and rural birds in blood and liver. Our results indicate the higher expression of a single caretaker gene (i.e. BRCA1) in livers of rural compared to urban birds. We conclude that, while urban birds might be exposed to an environment favoring the development of oncogenic processes, they seem to not upregulate their cancer defenses accordingly and future studies should confirm this result by assessing more markers of cancer defenses. This may result in a mismatch that might predispose urban birds to higher cancer risk and future studies in urban ecology should take into account this, so far completely ignored, hazard.


Assuntos
Neoplasias , Passeriformes , Animais , Ecossistema , Poluição Ambiental , Neoplasias/veterinária , Urbanização
9.
Transbound Emerg Dis ; 67(4): 1442-1446, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32068970

RESUMO

Reticuloendotheliosis virus (REV) has a wide range of avian hosts leading to reticuloendotheliosis, and its characteristic of vertical transmission makes it to be one of the most important diseases in breeder avian populations. Up to date, reports on neoplastic disease caused by REV in breeding ducks are few. Here, spontaneously occurring neoplastic disease caused by REV in breeding Muscovy ducks was reported in Guangdong province, China. The most significant gross lesions of sick ducks were tumour-bearing liver and enlarged spleen. Histopathological examination found proliferation of malignant lymphoreticular cells in the liver and reticuloendothelial cells in the spleen. REV strain, CH-GD2019, was successfully isolated using DF-1 cells, and the presence of REV was confirmed by PCR detection and transmission electron microscopy. The length of complete proviral genome is 8,238 nucleotides. Genetic and phylogenetic analyses revealed that CH-GD2019 was closely related to chicken-origin REV strains circulating in China. The results will provide a basic data for better understanding of REV in breeding ducks and suggest that REV from chickens may be a threat to ducks.


Assuntos
Galinhas/virologia , Neoplasias/veterinária , Doenças das Aves Domésticas/virologia , Vírus da Reticuloendoteliose/isolamento & purificação , Animais , Cruzamento , China/epidemiologia , Patos , Neoplasias/epidemiologia , Neoplasias/patologia , Neoplasias/virologia , Filogenia , Doenças das Aves Domésticas/epidemiologia , Doenças das Aves Domésticas/patologia , Vírus da Reticuloendoteliose/genética
10.
Vet Rec ; 186(1): 32-33, 2020 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-31919258
11.
Can J Vet Res ; 84(1): 60-66, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31949329

RESUMO

The urokinase plasminogen activator system (uPAS) has been poorly investigated in veterinary oncology. The aim of this study was to determine uPA serum concentrations in healthy and oncologic cats to understand the potential value of uPA as a cancer biomarker. Serum samples were collected from 19 healthy cats and 18 cats with spontaneous malignant neoplasms and uPA was measured through a specific enzyme-linked immunosorbent assay kit. The differences between uPA values and their relation with intrinsic factors and clinicopathological parameters were analyzed using an analysis of variance (ANOVA) and independent t-test. The average serum concentration of uPA in cancerous cats (0.54 ± 0.22 ng/mL) differed from that of healthy cats (1.10 ± 1.16 ng/mL) but was not significantly influenced by cats' clinicopathological parameters or by the presence of metastases. This study describes, for the first time, the serum concentrations of uPA in cats and proposes directions for future studies to uncover the relevance of uPAS in feline carcinogenesis.


Assuntos
Biomarcadores Tumorais/sangue , Doenças do Gato/sangue , Neoplasias/veterinária , Ativador de Plasminogênio Tipo Uroquinase/sangue , Análise de Variância , Animais , Estudos de Casos e Controles , Doenças do Gato/diagnóstico , Gatos , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Neoplasias/sangue , Neoplasias/diagnóstico , Estudos Prospectivos
12.
J Am Anim Hosp Assoc ; 56(2): 106-113, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31961212

RESUMO

The goal of this retrospective, cross-sectional study was to describe the different etiologies of trigeminal neuropathy based on clinical and MRI findings and to evaluate the significance of associated concomitant disorders. MRI studies of 49 dogs with trigeminal neuropathy were blindly reviewed and were classified into the following three groups: neoplasia, neuritis, or idiopathic trigeminal neuropathy (ITN). Thirty-one percent were suspected to have neoplasia (all unilateral), 16% to have neuritis (1 bilateral and 7 unilateral), and 53% to have ITN (4 unilateral and 22 bilateral). Dogs with clinical bilateral trigeminal dysfunction were most likely to have a diagnosis of ITN (predicted probability 95.7%). Unilateral clinical signs were significantly associated with neoplasia or neuritis compared with ITN (P < .001 and P = .002, respectively). Even with marked brainstem neoplastic involvement, central neurological deficits may be absent. Sensory impairment was significantly associated with either neoplasia or neuritis compared with ITN (P = .007 and P = .03, respectively). Ipsilateral noninfectious middle ear effusion was only seen in dogs with neoplasia (33%). Horner's syndrome was present in 12% of all dogs (2 dogs in each group). Dogs with neoplasia were significantly older than dogs with neuritis (P = .02) and ITN (P = .002). JAAHA-MS-6997.


Assuntos
Doenças do Cão/diagnóstico por imagem , Imagem por Ressonância Magnética/veterinária , Doenças do Nervo Trigêmeo/veterinária , Envelhecimento , Animais , Estudos Transversais , Doenças do Cão/diagnóstico , Doenças do Cão/patologia , Cães , Feminino , Imagem por Ressonância Magnética/métodos , Masculino , Neoplasias/diagnóstico por imagem , Neoplasias/veterinária , Neurite (Inflamação)/diagnóstico por imagem , Neurite (Inflamação)/veterinária , Estudos Retrospectivos , Doenças do Nervo Trigêmeo/diagnóstico por imagem , Doenças do Nervo Trigêmeo/patologia
13.
Sci Total Environ ; 712: 136512, 2020 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-31945522

RESUMO

Neoplasia and associated tissue biomarkers in benthic fishes are commonly used to characterize effects of contaminated sediments in aquatic ecosystems. However, these fish are often migratory or partially-migratory, and thus assessing the effect of location-specific contamination is challenging because the fish will have a complex exposure history. We determined liver and skin neoplasia prevalence for a benthic, partially-migratory fish, white sucker (Catostomus commersonii), and used carbon and nitrogen stable isotope ratios to determine the diet contribution associated with areas of contaminated sediments within the urbanized portion of the St. Louis River. We then tested which factors were significantly related to neoplasia prevalence, including age, sex, and the percent diet obtained from contaminated areas within the St. Louis River relative to Lake Superior, the reference area. Overall, the prevalence of contaminant-related internal and external tumors was low, <5%. For skin neoplasia prevalence, both sex and age were significant factors, whereas location-specific diet contribution based on stable isotope analysis was not a significant factor. For liver neoplasia prevalence, only age was a significant factor. Nevertheless, for all contaminants measured (polychlorinated biphenyls [PCBs], polychlorinated dibenzodioxins [PCDDs], and polychlorinated dibenzofurans [PCDFs]), there was a significant, negative correlation between liver tissue concentration and Lake Superior diet contribution, confirming that the St. Louis River is the primary source of contaminant exposure. The research highlights the complexity of exposure to location-specific contaminants and potentially infectious agents associated with neoplasia at urban, contaminated sites in the Great Lakes, and elsewhere. It also demonstrates the need to determine the full set of risk factors across life-stages, habitats, and biological endpoints.


Assuntos
Neoplasias/veterinária , Animais , Dibenzofuranos Policlorados , Ecossistema , Bifenilos Policlorados , Prevalência , Rios , Poluentes Químicos da Água
14.
Aust Vet J ; 98(4): 164-167, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31919837

RESUMO

A nine-year-old female desexed Great Dane presented with a painful, proliferative, soft red putative neoplastic vascular mass on the nictitating membrane. Three 7-day cycles of the topical cytotoxic drug mitomycin C 0.04%, applied four times daily to the lesion using a low-dose alternate-week pulse therapy, brought about rapid remission of the lesion. The lesion was still in remission at time of euthanasia some 13 months later.


Assuntos
Doenças do Cão/tratamento farmacológico , Mitomicina , Membrana Nictitante , Administração Tópica , Animais , Antibióticos Antineoplásicos/uso terapêutico , Cães , Feminino , Neoplasias/tratamento farmacológico , Neoplasias/veterinária
15.
J Vet Sci ; 21(1): e16, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31940695

RESUMO

Cancer is a major cause of death in dogs worldwide, and the incidence of cancer in dogs is increasing. The attenuated total reflection Fourier transform infrared spectroscopic (ATR-FTIR) technique is a powerful tool for the diagnosis of several diseases. This method enables samples to be examined directly without pre-preparation. In this study, we evaluated the diagnostic value of ATR-FTIR for the detection of cancer in dogs. Cancer-bearing dogs (n = 30) diagnosed by pathologists and clinically healthy dogs (n = 40) were enrolled in this study. Peripheral blood was collected for clinicopathological diagnosis. ATR-FTIR spectra were acquired, and principal component analysis was performed on the full wave number spectra (4,000-650 cm-1). The leave-one-out cross validation technique and partial least squares regression analysis were used to predict normal and cancer spectra. Red blood cell counts, hemoglobin levels and white blood cell counts were significantly lower in cancer-bearing dogs than in clinically healthy dogs (p < 0.01, p < 0.01 and p = 0.03, respectively). ATR-FTIR spectra showed significant differences between the clinically healthy and cancer-bearing groups. This finding demonstrates that ATR-FTIR can be applied as a screening technique to distinguish between cancer-bearing dogs and healthy dogs.


Assuntos
Contagem de Células Sanguíneas/veterinária , Análise Química do Sangue/veterinária , Sangue/parasitologia , Doenças do Cão/diagnóstico , Neoplasias/veterinária , Espectroscopia de Infravermelho com Transformada de Fourier/veterinária , Animais , Cães , Feminino , Masculino , Neoplasias/diagnóstico , Espectroscopia de Infravermelho com Transformada de Fourier/instrumentação , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Tailândia
16.
Funct Integr Genomics ; 20(1): 75-87, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31368028

RESUMO

Long non-coding RNA (lncRNA) was previously considered as a non-functional transcript, which now established as part of regulatory elements of biological events such as chromosome structure, remodeling, and regulation of gene expression. The study presented here showed the role of lncRNA through differential expression analysis on cancer-related coding genes in horn squamous cell carcinoma of Indian zebu cattle. A total of 10,360 candidate lncRNAs were identified and further analyzed for its coding potential ability using three tools (CPC, CPAT, and PLEK) that provide 8862 common lncRNAs. Pfam analysis of these common lncRNAs gave 8612 potential candidates for lncRNA differential expression analysis. Differential expression analysis showed a total of 59 significantly differentially expressed genes and 19 lncRNAs. Pearson's correlation analysis was used to identify co-expressed mRNA-lncRNAs to established relation of the regulatory role of lncRNAs in horn cancer. We established a positive relation of seven upregulated (XLOC_000016, XLOC_002198, XLOC_002851, XLOC_ 007383, XLOC_010701, XLOC_010272, and XLOC_011517) and one downregulated (XLOC_011302) lncRNAs with eleven genes that are related to keratin family protein, keratin-associated protein family, cornifelin, corneodesmosin, serpin family protein, and metallothionein that have well-established role in squamous cell carcinoma through cellular communication, cell growth, cell invasion, and cell migration. These biological events were found to be related to the MAPK pathway of cell cycle regulation indicating the role of lncRNAs in manipulating cell cycle regulation during horn squamous cell carcinomas that will be useful in identifying molecular portraits related to the development of horn cancer.


Assuntos
Doenças dos Bovinos/genética , Cornos , Neoplasias/veterinária , RNA Longo não Codificante/metabolismo , Animais , Bovinos , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Neoplasias/genética , RNA Longo não Codificante/fisiologia , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase em Tempo Real
17.
Vet Rec ; 186(1): 28, 2020 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-31554709

RESUMO

BACKGROUND: Neoplasia has historically been regarded as an unusual occurrence in reptiles. The aim of the present study was to evaluate the prevalence of neoplasms in routine diagnostic samples submitted to a specialist exotic animal laboratory. METHODS: Over a 10-year period, 690 lizard samples were submitted for histopathological or cytopathological examination by Royal College of Veterinary Surgeons specialists in Veterinary Pathology (Zoo and Wildlife). Records were reviewed retrospectively and non-neoplastic diagnoses excluded from further analysis. RESULTS: 158 neoplasms were diagnosed in 149 lizards, with 22.9% of submissions resulting in a diagnosis of neoplasia. The skin was the most commonly affected organ, and squamous cell carcinomas were the most common neoplasms identified (17.7% of all neoplastic diagnoses).Bearded dragons and panther chameleons had a statistically significant higher proportion of neoplasia diagnosis than the lizard population as a whole, and geckos had a statistically significantly lower proportion.Several neoplasms identified in this study have not been previously reported in lizards, including a testicular Sertoli cell tumour, testicular granulosa cell tumour, splenic haemangiosarcoma, gastric adenocarcinoma and basal cell carcinoma. CONCLUSION: The results suggest that the prevalence of neoplasia in captive lizards may be higher than previously reported, and that there is variation in prevalence between different lizard species and families. It is unclear whether these differences relate to genetic or management factors.


Assuntos
Animais de Zoológico , Lagartos , Neoplasias/diagnóstico , Neoplasias/veterinária , Animais , Neoplasias/epidemiologia , Prevalência , Estudos Retrospectivos , Reino Unido/epidemiologia
18.
Vet Surg ; 49(1): 61-69, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31290167

RESUMO

OBJECTIVE: To evaluate reporting of surgical complications and other adverse events in clinical research articles describing soft tissue and oncologic surgery in dogs and cats. STUDY DESIGN: Systematic literature review. SAMPLE: English-language articles describing soft tissue and oncologic surgeries in client-owned dogs and cats published in peer-reviewed journals from 2013 to 2016. METHODS: CAB, AGRICOLA, and MEDLINE databases were searched for eligible articles. Article characteristics relevant to complications were abstracted and summarized, including reported events, definitions, criteria used to classify events according to severity and time frame, and relevant citations. RESULTS: One hundred fifty-one articles involving 10 522 animals were included. Canine retrospective case series of dogs predominated. Ninety-two percent of articles mentioned complications in study results, but only 7.3% defined the term complication. Articles commonly described complications according to time frame and severity, but terminology and classification criteria were highly variable, conflicting between studies, or not provided. Most (58%) reported complications could have been graded with a published veterinary adverse event classification scheme, although common intraoperative complications were notable exceptions. CONCLUSION: Definitions and criteria used to classify and report soft tissue and oncologic surgical complications are often absent, incomplete, or contradictory among studies. CLINICAL SIGNIFICANCE: Lack of consistent terminology contributes to inadequate communication of important information about surgical complications. Standardization of terminology and consistency in severity scoring will improve comparative evaluation of clinical research results.


Assuntos
Doenças do Gato/cirurgia , Doenças do Cão/cirurgia , Neoplasias/veterinária , Complicações Pós-Operatórias/veterinária , Sistemas de Notificação de Reações Adversas a Medicamentos , Animais , Gatos , Cães , Neoplasias/cirurgia , Projetos de Pesquisa , Estudos Retrospectivos
19.
Mol Biol Evol ; 37(1): 11-17, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31688937

RESUMO

Despite a considerable expenditure of time and resources and significant advances in experimental models of disease, cancer research continues to suffer from extremely low success rates in translating preclinical discoveries into clinical practice. The continued failure of cancer drug development, particularly late in the course of human testing, not only impacts patient outcomes, but also drives up the cost for those therapies that do succeed. It is clear that a paradigm shift is necessary if improvements in this process are to occur. One promising direction for increasing translational success is comparative oncology-the study of cancer across species, often involving veterinary patients that develop naturally-occurring cancers. Comparative oncology leverages the power of cross-species analyses to understand the fundamental drivers of cancer protective mechanisms, as well as factors contributing to cancer initiation and progression. Clinical trials in veterinary patients with cancer provide an opportunity to evaluate novel therapeutics in a setting that recapitulates many of the key features of human cancers, including genomic aberrations that underly tumor development, response and resistance to treatment, and the presence of comorbidities that can affect outcomes. With a concerted effort from basic scientists, human physicians and veterinarians, comparative oncology has the potential to enhance the cost-effectiveness and efficiency of pipelines for cancer drug discovery and other cancer treatments.


Assuntos
Descoberta de Drogas , Neoplasias/veterinária , Animais , Humanos , Neoplasias/tratamento farmacológico
20.
Avian Pathol ; 49(2): 202-207, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31702386

RESUMO

Marek's disease (MD) is a lymphoproliferative disease caused by Gallid alphaherpesvirus 2 (GaHV-2), which primarily affects chickens. However, the virus is also able to induce tumours in turkeys, albeit less frequently than in chickens. This study reports the molecular characterization of a GaHV-2 strain detected in a flock of Italian meat-type turkeys exhibiting visceral lymphomas. Sequencing and phylogenetic analysis of the meq gene revealed that the turkey GaHV-2 has molecular features of high virulence and genetic similarity with GaHV-2 strains recently detected in Italian commercial and backyard chickens. GaHV-2 is ubiquitous among chickens despite vaccination, and chicken-to-turkey transmission is hypothesized due to the presence of broilers in neighbouring pens.RESEARCH HIGHLIGHTS A GaHV-2 strain from Italian turkeys was molecularly characterized.The turkey strain presented molecular characteristics of high virulence in its meq gene.The turkey strain was closely related to previously detected chicken strains.


Assuntos
Herpesvirus Galináceo 2 , Doença de Marek/virologia , Neoplasias/veterinária , Perus , Animais , Regulação Viral da Expressão Gênica , Herpesvirus Galináceo 2/genética , Doença de Marek/patologia , Neoplasias/virologia , Proteínas Oncogênicas Virais/isolamento & purificação , Filogenia , Reação em Cadeia da Polimerase/veterinária , Doenças das Aves Domésticas/virologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...