Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 32
Filtrar
Mais filtros










Tipo de estudo
Intervalo de ano de publicação
1.
Neurol Res ; 41(4): 326-334, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30638158

RESUMO

OBJECTIVE: To verify the action of sericin associated to swim exercise with overload, on sciatic nerve repair in Wistar rats, after 22 days of nerve compression. METHODS: Forty animals been composed of five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the lesion procedure, sericin, in hydrolyzed form, applied directly to the injury in the injury-sericin and injury-sericin-swimming groups. Injury-swimming and injury-sericin-swimming groups underwent to 5 days per week for 3 weeks, with a 10% overload of the animal's body weight, and a weekly progressive evolution of swimming time, lasting 15, 20 and 25 min/day. Pre and throughout the treatment period the animals performed evaluation of sciatic functional index and pressure pain threshold with digital von Frey filament. Euthanasia was performed on the 22nd postoperative day, and two fragments of the nerve were collected and prepared for descriptive and quantitative analysis. RESULTS: The sciatic functional index assessment showed significant differences in the motor function of the control until the 14th day. Regarding the allodynia, there was revealed a significant improvement in injury-swimming performance relative to injury, injury-sericin and injury-sericin-swimming, and the number of viable and non-viable nerve fibers smaller than 4 µm in diameter was significantly higher in the injury-sericin-swimming. CONCLUSION: swimming showed a better evolution of the nociceptive threshold and allodynia. Sericin treatment had exacerbated pro-inflammatory characteristics. On the other hand, the association of sericine and swimming showed a possible regulatory effect by resting swimming exercise, with a significant increase of fibers of smaller diameter.


Assuntos
Condicionamento Físico Animal/métodos , Neuropatia Ciática/tratamento farmacológico , Neuropatia Ciática/reabilitação , Sericinas/uso terapêutico , Natação , Animais , Modelos Animais de Doenças , Medição da Dor , Limiar da Dor/efeitos dos fármacos , Limiar da Dor/fisiologia , Ratos , Ratos Wistar , Nervo Isquiático/patologia , Neuropatia Ciática/fisiopatologia , Sericinas/metabolismo , Índice de Gravidade de Doença , Estatísticas não Paramétricas , Resultado do Tratamento
2.
Medicine (Baltimore) ; 97(23): e11051, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29879074

RESUMO

RATIONALE: Peripheral neuropathy is a rare complication of carbon monoxide intoxication. Peripheral neuropathy following carbon monoxide intoxication is known to completely recover within a few months. PATIENT CONCERNS: A 40-year-old man complained of motor weakness and hypoesthesia of the right lower extremity with swelling of his right thigh after carbon monoxide intoxication resulting from a suicide attempt. DIAGNOSES: Following nerve conduction and electromyographic studies, the patient was diagnosed with sciatic neuropathy with severe axonopathy. Clinical and laboratory findings led to a diagnosis of rhabdomyolysis. INTERVENTIONS: The patient was treated conservatively for rhabdomyolysis and underwent comprehensive rehabilitation for sciatic neuropathy during hospitalization. OUTCOMES: After discharge, he underwent serial follow-up tests with nerve conduction and electromyographic studies, which showed prolonged persistence of sciatic neuropathy; however, he showed significant improvement at his 26-month post-discharge follow-up. LESSON: Patients presenting with peripheral neuropathy secondary to carbon monoxide intoxication may show variable recovery periods; however, a favorable prognosis can be expected regardless of the concomitant occurrence of rhabdomyolysis and/or compartment syndrome.


Assuntos
Intoxicação por Monóxido de Carbono/complicações , Monóxido de Carbono/toxicidade , Rabdomiólise/etiologia , Neuropatia Ciática/etiologia , Adulto , Síndromes Compartimentais/diagnóstico , Síndromes Compartimentais/etiologia , Eletromiografia/métodos , Humanos , Masculino , Condução Nervosa/fisiologia , Doenças do Sistema Nervoso Periférico/etiologia , Doenças do Sistema Nervoso Periférico/fisiopatologia , Rabdomiólise/diagnóstico , Rabdomiólise/terapia , Neuropatia Ciática/fisiopatologia , Neuropatia Ciática/reabilitação , Tentativa de Suicídio/psicologia , Resultado do Tratamento
3.
Pain ; 159(3): 437-450, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29140923

RESUMO

Peripheral nerve injury (PNI) activates the immune system, resulting in increased proinflammatory cytokines at the site of injury and in the spinal cord dorsal horn. Exercise modulates the immune system promoting an anti-inflammatory phenotype of macrophages in uninjured muscle, and increases in anti-inflammatory cytokines can promote healing and analgesia. We proposed that PNI will decrease, and treadmill exercise will increase, release of anti-inflammatory cytokines at the site of injury and in the spinal cord. We show that 2 weeks of treadmill exercise improves neuropathic pain behaviors in mice: mechanical hyperalgesia, escape and avoidance behavior, and spontaneous locomotor activity. Peripheral nerve injury reduced anti-inflammatory cytokines (interleukin-4 [IL-4], IL-1ra, and IL-5) at the site of nerve injury and in the spinal dorsal horn, whereas exercise restored IL-4, IL-1ra, and IL-5 concentrations to preinjury levels. IL4 mice and mice treated with IL-4 antibody did not develop analgesia to treadmill exercise. Using immunohistochemical staining of the sciatic nerve, treadmill exercise increased the percentage of M2 macrophages (secretes anti-inflammatory cytokines) and decreased M1 macrophages (secretes proinflammatory cytokines) when compared with sedentary mice. The increased M2 and decreased M1 macrophages in exercised mice did not occur in IL-4 mice. In the spinal cord, PNI increased glial cell activation, brain-derived neurotrophic factor and ß-nerve growth factor levels, and decreased IL-4 and IL-1ra levels, whereas treadmill exercise suppressed glial cells activation (Glial Fibrillary Acidic Protein and Iba1 immunoreactivity), reduced brain-derived neurotrophic factor and ß-nerve growth factor, and increased IL-4, IL-1ra, and IL-5 concentrations. Our results suggest that IL-4 mediates the analgesia produced by low-intensity exercise by modulating peripheral and central neuroimmune responses in mice with neuropathic pain.


Assuntos
Interleucina-4/metabolismo , Condicionamento Físico Animal/métodos , Neuropatia Ciática/patologia , Neuropatia Ciática/reabilitação , Medula Espinal/metabolismo , Animais , Anticorpos/uso terapêutico , Aprendizagem da Esquiva/fisiologia , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Citocinas/metabolismo , Modelos Animais de Doenças , Teste de Esforço , Comportamento Exploratório/fisiologia , Regulação da Expressão Gênica/fisiologia , Hiperalgesia/tratamento farmacológico , Hiperalgesia/etiologia , Interleucina-4/genética , Interleucina-4/imunologia , Masculino , Camundongos , Camundongos Knockout , Fator de Crescimento Neural/metabolismo , Neuroglia/efeitos dos fármacos , Neuroglia/metabolismo , Limiar da Dor/fisiologia , Neuropatia Ciática/tratamento farmacológico , Neuropatia Ciática/fisiopatologia , Medula Espinal/efeitos dos fármacos , Medula Espinal/patologia , Fatores de Tempo
4.
Einstein (Sao Paulo) ; 15(2): 186-191, 2017.
Artigo em Inglês, Português | MEDLINE | ID: mdl-28767917

RESUMO

Objective: To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods: Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results: The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion: The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle. Objetivo: Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos: Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados: A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão: O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.


Assuntos
Anti-Inflamatórios/farmacologia , Benzaldeídos/farmacologia , Tecido Conjuntivo/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Neuropatia Ciática/patologia , Animais , Tecido Conjuntivo/patologia , Humanos , Masculino , Modelos Animais , Fibras Musculares Esqueléticas/efeitos dos fármacos , Músculo Esquelético/patologia , Distribuição Aleatória , Ratos Wistar , Neuropatia Ciática/reabilitação
5.
Einstein (Säo Paulo) ; 15(2): 186-191, Apr.-June 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-891383

RESUMO

ABSTRACT Objective To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle.


RESUMO Objetivo Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.


Assuntos
Humanos , Animais , Masculino , Benzaldeídos/farmacologia , Músculo Esquelético/efeitos dos fármacos , Tecido Conjuntivo/efeitos dos fármacos , Neuropatia Ciática/patologia , Anti-Inflamatórios/farmacologia , Distribuição Aleatória , Ratos Wistar , Músculo Esquelético/patologia , Fibras Musculares Esqueléticas/efeitos dos fármacos , Tecido Conjuntivo/patologia , Neuropatia Ciática/reabilitação , Modelos Animais
6.
J Minim Invasive Gynecol ; 24(5): 822-826, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28445777

RESUMO

OBJECTIVE: To report neurologic follow-up of patients after laparoscopic large resection of deep infiltrating endometriosis of the sciatic nerve. DESIGN: Prospective clinical case series. SETTING: Tertiary referral unit specializing in advanced gynecologic surgery and neuropelveology. PATIENTS: All data for patients who underwent laparoscopic surgery for endometriosis of the sciatic nerve between 2004 and 2016 (n = 259) were documented prospectively. In this study, patients who underwent a large resection of the sciatic nerve (>30% of the nerve) and were followed for at least 5 years were evaluated (n = 46). All patients presented preoperatively with incapacity for normal gait and foot drop. All were suffering from intractable and constant neuropathic sciatic pain (visual analog scale [VAS] score of 9 to 10 despite strong pain medicine), with sensorimotor disorders of the affected leg. INTERVENTIONS: Laparoscopic large resection of endometriosis of the sciatic nerve. MEASUREMENTS AND MAIN RESULTS: All procedures were performed by laparoscopy. Postoperative management included medical treatment with neuroleptic agents and intensive physiotherapy. At the 5-year follow-up, all patients reported significant pain reduction, with a median VAS score of 2.1 (range, 0 to 3) and recovery of normal gait, including the ability to climb stairs. CONCLUSION: In deep infiltrating intraneural endometriosis of the sciatic nerve, patients present with motor disorders before and after surgical resection. The average VAS score was reduced from 9.33 preoperatively to 1.25 at a 3-year follow-up. When full resection of endometriosis including nerve resection is completed, sciatic nerve function recover, but recovery of a normal gait may take at least 3 years and intensive physiotherapy.


Assuntos
Endometriose/cirurgia , Procedimentos Cirúrgicos em Ginecologia/métodos , Laparoscopia , Nervo Isquiático/cirurgia , Neuropatia Ciática/cirurgia , Adulto , Endometriose/patologia , Feminino , Seguimentos , Transtornos Neurológicos da Marcha/etiologia , Transtornos Neurológicos da Marcha/reabilitação , Transtornos Neurológicos da Marcha/cirurgia , Procedimentos Cirúrgicos em Ginecologia/efeitos adversos , Humanos , Laparoscopia/efeitos adversos , Laparoscopia/métodos , Dor/etiologia , Dor/reabilitação , Doenças Peritoneais/etiologia , Doenças Peritoneais/reabilitação , Doenças Peritoneais/cirurgia , Recuperação de Função Fisiológica , Nervo Isquiático/patologia , Nervo Isquiático/fisiopatologia , Neuropatia Ciática/patologia , Neuropatia Ciática/reabilitação , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
7.
Pain Res Manag ; 2016: 6487160, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27594795

RESUMO

Background. To investigate the climb stairs resistance exercise on nociception and axonal regeneration in the sciatic nerve of rats. Methods. 24 Wistar rats were divided: control group (CG-no injury), exercise group (EG-no injury with physical exercise), lesion group (LG-injury, but without exercise), and treated group (LEG-injury and physical exercise). LG and LEG were subjected to sciatic nerve compression with hemostat. From the 3rd day after injury began treatment with exercise, and after 22 days occurs the removal of a nerve fragment for morphological analysis. Results. Regarding allodynia, CG obtained values less than EG (p = 0.012) and larger than LG and LEG (p < 0.001). Histological results showed that CG and EG had normal appearance, as LG and LEG showed up with large amounts of inflammatory infiltration, degeneration and disruption of nerve fibers, and reduction of the myelin sheath; however LEG presented some regenerated fibers. From the morphometric data there were significant differences, for nerve fiber diameter, comparing CG with LG and LEG and comparing axon diameter and the thickness of the myelin of the CG to others. Conclusion. Climb stairs resistance exercise was not effective to speed up the regenerative process of axons.


Assuntos
Artrogripose/complicações , Axônios/patologia , Terapia por Exercício , Neuropatia Hereditária Motora e Sensorial/complicações , Neuropatia Ciática , Análise de Variância , Animais , Modelos Animais de Doenças , Hiperalgesia/diagnóstico , Hiperalgesia/etiologia , Fibras Nervosas/patologia , Limiar da Dor , Condicionamento Físico Animal , Modalidades de Fisioterapia , Ratos , Ratos Wistar , Neuropatia Ciática/etiologia , Neuropatia Ciática/patologia , Neuropatia Ciática/reabilitação
8.
J Pain ; 17(5): 588-99, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-26844418

RESUMO

UNLABELLED: Physical exercise can attenuate neuropathic pain (NPP), but the exact mechanism underlying exercise-induced hypoalgesia (EIH) remains unclear. Recent studies have shown that histone hyperacetylation via pharmacological inhibition of histone deacetylases in the spinal cord attenuates NPP, and that histone acetylation may lead to the production of analgesic factors including interleukin 10. We intended to clarify whether histone acetylation in microglia in the spinal dorsal horn contributes to EIH in NPP model mice. C57BL/6J mice underwent partial sciatic nerve ligation (PSL) and PSL- and sham-runner mice ran on a treadmill at a speed of 7 m/min for 60 min/d, 5 days per week, from 2 days after the surgery. PSL-sedentary mice developed mechanical allodynia and heat hyperalgesia, but such behaviors were significantly attenuated in PSL-runner mice. In immunofluorescence analysis, PSL surgery markedly increased the number of histone deacetylase 1-positive/CD11b-positive microglia in the ipsilateral superficial dorsal horn, and they were significantly decreased by treadmill-running. Moreover, the number of microglia with nuclear expression of acetylated H3K9 in the ipsilateral superficial dorsal horn was maintained at low levels in PSL-sedentary mice, but running exercise significantly increased them. Therefore, we conclude that the epigenetic modification that causes hyperacetylation of H3K9 in activated microglia may play a role in producing EIH. PERSPECTIVE: This article presents the importance of epigenetic modification in microglia in producing EIH. The current research is not only helpful for developing novel nonpharmacological therapy for NPP, but will also enhance our understanding of the mechanisms and availability of exercise in our daily life.


Assuntos
Histonas/metabolismo , Hiperalgesia/etiologia , Hiperalgesia/patologia , Microglia/metabolismo , Condicionamento Físico Animal/efeitos adversos , Neuropatia Ciática/reabilitação , Acetilação , Animais , Antígeno CD11b/metabolismo , Modelos Animais de Doenças , Teste de Esforço , Lateralidade Funcional , Proteína Glial Fibrilar Ácida/metabolismo , Histona Desacetilase 1/metabolismo , Interleucina-10/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Medição da Dor , Fosfopiruvato Hidratase/metabolismo , Estimulação Física , Neuropatia Ciática/fisiopatologia , Estatísticas não Paramétricas
9.
Braz J Med Biol Res ; 49(2): e5124, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26871970

RESUMO

This study evaluated the effect of muscle satellite cells (MSCs) overexpressing myogenin (MyoG) on denervated muscle atrophy. Rat MSCs were isolated and transfected with the MyoG-EGFP plasmid vector GV143. MyoG-transfected MSCs (MTMs) were transplanted into rat gastrocnemius muscles at 1 week after surgical denervation. Controls included injections of untransfected MSCs or the vehicle only. Muscles were harvested and analyzed at 2, 4, and 24 weeks post-transplantation. Immunofluorescence confirmed MyoG overexpression in MTMs. The muscle wet weight ratio was significantly reduced at 2 weeks after MTM injection (67.17±6.79) compared with muscles injected with MSCs (58.83±5.31) or the vehicle (53.00±7.67; t=2.37, P=0.04 and t=3.39, P=0.007, respectively). The muscle fiber cross-sectional area was also larger at 2 weeks after MTM injection (2.63×10³±0.39×10³) compared with MSC injection (1.99×10³±0.58×10³) or the vehicle only (1.57×10³±0.47×10³; t=2.24, P=0.049 and t=4.22, P=0.002, respectively). At 4 and 24 weeks post-injection, the muscle mass and fiber cross-sectional area were similar across all three experimental groups. Immunohistochemistry showed that the MTM group had larger MyoG-positive fibers. The MTM group (3.18±1.13) also had higher expression of MyoG mRNA than other groups (1.41±0.65 and 1.03±0.19) at 2 weeks after injection (t=2.72, P=0.04). Transplanted MTMs delayed short-term atrophy of denervated muscles. This approach can be optimized as a novel stand-alone therapy or as a bridge to surgical re-innervation of damaged muscles.


Assuntos
Transplante de Células , Denervação Muscular/reabilitação , Músculo Esquelético/inervação , Atrofia Muscular/reabilitação , Miogenina/metabolismo , Células Satélites de Músculo Esquelético/transplante , Animais , Imunofluorescência , Expressão Gênica , Masculino , Atrofia Muscular/etiologia , Miogenina/genética , Tamanho do Órgão/genética , Plasmídeos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Células Satélites de Músculo Esquelético/citologia , Células Satélites de Músculo Esquelético/metabolismo , Neuropatia Ciática/reabilitação , Transfecção
10.
Muscle Nerve ; 51(1): 83-91, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24752648

RESUMO

INTRODUCTION: Different rehabilitation treatments have proven useful in accelerating regeneration. METHODS: After sciatic nerve crush in rats, we tested balance and coordination training (BCT) and endurance training (ET) through sensorimotor tests and analyzed nerve and muscle morphology. RESULTS: After BCT and ET, rats performed better in sensorimotor tests than did non-trained animals. However, only BCT maintained sensorimotor function during training. Furthermore, BCT and ET produced significantly larger muscle area than in non-trained animals. CONCLUSIONS: These findings indicate that BCT and ET, when initiated in the early phase after sciatic nerve injury, improve morphological properties of the soleus muscle and sciatic nerve, but only the task-oriented BCT maintained sensorimotor function. The success of rehabilitative strategies appears to be highly task-specific, and strategies that stimulate sensory pathways are the most effective in improving balance and/or coordination parameters.


Assuntos
Terapia por Exercício/métodos , Resistência Física/fisiologia , Equilíbrio Postural/fisiologia , Desempenho Psicomotor/fisiologia , Neuropatia Ciática/fisiopatologia , Neuropatia Ciática/reabilitação , Animais , Modelos Animais de Doenças , Locomoção/fisiologia , Masculino , Músculo Esquelético/patologia , Compressão Nervosa/métodos , Fibras Nervosas Mielinizadas/patologia , Ratos , Ratos Wistar , Recuperação de Função Fisiológica/fisiologia , Neuropatia Ciática/patologia
12.
J Emerg Med ; 43(4): 622-4, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20580878

RESUMO

BACKGROUND: Presentations of lower extremity compression palsies are rare and exist only in case reports. The true incidence of emergency department (ED) presentations of bilateral sciatic nerve palsy, "toilet bowl neuropathy," is unknown. The following is a case of a 28-year-old active duty Navy woman who presented to our ED via ambulance with bilateral sciatic nerve palsy from prolonged immobility. OBJECTIVES: This discussion reviews the historical precedence for the diagnosis and details the work-up and prognosis. CASE REPORT: The patient presented complaining of bilateral lower extremity swelling, numbness, and an inability to walk after a prolonged, medication-facilitated sleep in an unusual position. She had significant weakness on examination and was admitted for further evaluation and care. Specialized neurologic studies were consistent with bilateral sciatic nerve palsy. CONCLUSION: Bilateral sciatic nerve palsy is an uncommon cause of lower extremity weakness. In the ED, other diagnoses such as Guillain-Barré syndrome, cauda equina, disk herniation, and gluteal compartment syndrome should be considered initially. The diagnosis of toilet bowl neuropathy, however, is dependent on obtaining an accurate history. This case report adds another perspective to the literature on sciatic nerve palsy recognition, diagnosis, and prognosis.


Assuntos
Debilidade Muscular/etiologia , Pressão/efeitos adversos , Neuropatia Ciática/etiologia , Adulto , Edema/etiologia , Feminino , Humanos , Hipestesia/etiologia , Extremidade Inferior/inervação , Extremidade Inferior/fisiopatologia , Debilidade Muscular/fisiopatologia , Debilidade Muscular/reabilitação , Músculo Esquelético/inervação , Músculo Esquelético/fisiopatologia , Postura , Neuropatia Ciática/fisiopatologia , Neuropatia Ciática/reabilitação
13.
Acta ortop. bras ; 20(2): 93-97, 2012. ilus, tab
Artigo em Português | LILACS | ID: lil-622370

RESUMO

OBJETIVO: Analisar a eficiência do tratamento com a estimulação elétrica de alta voltagem (EEAV) em três diferentes locais, aplicada precocemente na regeneração do nervo ciático submetido à lesão por esmagamento, e avaliada através do índice funcional do ciático (IFC), em ratos. MÉTODO: Após o esmagamento, 57 ratos foram submetidos à EEAV catódica nos parâmetros: frequência de 50Hz, 100V de tensão, 20 minutos diários, 5 dias por semana. Os ratos foram divididos aleatoriamente em: grupo controle; grupo gânglio; grupo gânglio + músculo; grupo músculo e; grupo simulado. O IFC foi avaliado semanalmente durante sete semanas, partindo do pré-operatório até a 6ª semana pós-operatória. RESULTADOS: Em comparação ao grupo controle, os resultados mostraram desempenho significativamente superior do grupo gânglio nas três primeiras semanas, e do grupo gânglio + músculo na 3ª semana, enquanto o grupo músculo teve desempenho significativamente negativo na 4ª e 6ª semanas. CONCLUSÃO: a EEAV aplicada precocemente, foi positiva no tratamento da região da medula e gânglio da raiz nervosa do ciático com o eletrodo dispersivo na região lombar contralateral ou no músculo gastrocnêmio. Porém, proporcionou efeitos negativos no tratamento com eletrodo ativo no músculo gastrocnêmio e dispersivo na coxa contralateral. Nível de evidência II, Estudo prospectivo comparativo.


OBJECTIVE: To analyze the efficiency of high voltage pulsed current (HVPC) with early application in three different sites, in the regeneration of the sciatic nerve in rats submitted to crush injury, the sciatic functional index (SFI) was used to assess the functional recovery. METHODS: After crushing of the nerve, 57 animals were submitted to cathodal HVPC at frequency of 50Hz and voltage of 100V, 20 minutes per day, 5 days per week. The rats were divided into five groups: control group; ganglion group; ganglion + muscle group; muscle group; and sham group. The SFI was determined weekly for seven weeks, from the preoperative period to the 6th postoperative week. RESULTS: Compared with the control group, the results showed a significantly better performance of group 2 for the first 3 weeks; group 3 showed significantly better performance in the third week; and group 4 showed a significantly negative performance during the 4th and 6th weeks. CONCLUSION: Early application of HVPC had a positive effect in the treatment of the spinal cord region and the sciatic nerve root ganglion with a dispersive electrode on the contralateral lumbar region or on the gastrocnemius. However, HVPC had a negative effect in the treatment with an active electrode on the gastrocnemius and a dispersive electrode on the contralateral thigh. Level of evidence II, Prospective comparative study.


Assuntos
Masculino , Ratos , Região Lombossacral , Nervo Isquiático/lesões , Neuropatia Ciática/reabilitação , Medula Espinal , Estimulação Elétrica Nervosa Transcutânea , Regeneração Nervosa , Ratos Wistar
14.
Behav Brain Res ; 225(2): 562-73, 2011 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-21875621

RESUMO

Walking analysis in the rat is increasingly used to assess functional recovery after peripheral nerve injury. Here we assess the sensitivity and specificity of hindlimb joint kinematics measures during the rat gait early after sciatic nerve crush injury (DEN), after twelve weeks of recovery (REINN) and in sham-operated controls (Sham) using discriminant analysis. The analysis addressed gait spatiotemporal variables and hip, knee and ankle angle and angular velocity measures during the entire walking cycle. In DEN animals, changes affected all studied joints plus spatiotemporal parameters of gait. Both the spatiotemporal and ankle kinematics parameters recovered to normality within twelve weeks. At this time point, some hip and knee kinematics values were still abnormal when compared to sham controls. Discriminant models based on hip, knee and ankle kinematics displayed maximal sensitivity to identify DEN animals. However, the discriminant models based on spatiotemporal and ankle kinematics data showed a poor performance when assigning animals to the REINN and Sham groups. Models using hip and knee kinematics during walking showed the best sensitivity to recognize the reinnervated animals. The model construed on the basis of hip joint kinematics was the one combining highest sensitivity with robustness and high specificity. It is concluded that ankle joint kinematics fails in detecting minor functional deficits after long term recovery from sciatic nerve crush and extending the kinematic analysis during walking to the hip and knee joints improves the sensitivity of this functional test.


Assuntos
Fenômenos Biomecânicos/fisiologia , Marcha/fisiologia , Membro Posterior/fisiologia , Articulações/fisiologia , Compressão Nervosa/reabilitação , Recuperação de Função Fisiológica/fisiologia , Neuropatia Ciática/fisiopatologia , Animais , Análise Discriminante , Masculino , Modelos Estatísticos , Ratos , Ratos Sprague-Dawley , Neuropatia Ciática/diagnóstico , Neuropatia Ciática/reabilitação , Sensibilidade e Especificidade , Fatores de Tempo
15.
Muscle Nerve ; 44(1): 55-62, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21488054

RESUMO

INTRODUCTION: Numerous therapeutic interventions have been tested to enhance functional recovery after peripheral nerve injuries. METHODS: After sciatic nerve crush in rats we tested balance and coordination and motor control training in sensorimotor tests and analyzed nerve and muscle histology. RESULTS: The balance and coordination training group and the sham group had better results than the sedentary and motor control groups in sensorimotor tests. The sham and balance and coordination groups had a significantly larger muscle area than the other groups, and the balance and coordination group showed significantly better values than the sedentary and motor control groups for average myelin sheath thickness and g-ratio of the distal portion of the nerve. CONCLUSIONS: The findings indicate that balance and coordination training improves sciatic nerve regeneration, suggesting that it is possible to revert and/or prevent soleus muscle atrophy and improve performance on sensorimotor tests.


Assuntos
Terapia por Exercício , Equilíbrio Postural/fisiologia , Desempenho Psicomotor/fisiologia , Recuperação de Função Fisiológica/fisiologia , Neuropatia Ciática/fisiopatologia , Neuropatia Ciática/reabilitação , Animais , Terapia por Exercício/métodos , Masculino , Distribuição Aleatória , Ratos , Ratos Wistar
16.
Rev. bras. med. esporte ; 17(2): 115-118, mar.-abr. 2011. ilus
Artigo em Português | LILACS | ID: lil-591376

RESUMO

OBJETIVO: Avaliar a eficácia do exercício físico (natação e salto) atuando na redução do quadro álgico de ratos submetidos a um modelo experimental de ciatalgia. MÉTODOS: 24 ratos Wistar foram divididos em quatro grupos: grupo Placebo (GP), grupo Natação (GN), grupo Natação 10 por cento (GN10) e grupo Salto (GS). Todos os grupos foram submetidos ao modelo de ciatalgia e avaliados em relação à dor pós-exercício pelo teste de incapacidade funcional e pelo filamento de Von Frey. RESULTADOS: Na comparação intragrupos houve diferenças significativas, para todos os grupos, nos momentos pós-lesão comparados com o pré-lesão, por ambos os instrumentos de avaliação. Com o filamento de Von Frey observou-se diferença significativa nos grupos GN10 e GS nos momentos finais de avaliação. Na comparação intergrupos não houve diferenças significativas com nenhum instrumento de avaliação. CONCLUSÃO: O tratamento com exercício físico não foi eficaz para reduzir o quadro álgico de ratos submetidos à ciatalgia.


OBJECTIVE: To evaluate the efficacy of physical exercise (swimming and jumping), with and without overload, working in reducing the pain of rats undergone to an experimental model of sciatica. METHODS: 24 rats were divided into four groups: Placebo (GP), Swimming Group (NG) Swimming 10 percent Group (GN10) and Leap Group (GS). All groups were submitted to the experimental sciatica model and assessed for pain post-exercise for the Functional Disability Test and the Von Frey filament. RESULTS: In comparison within groups there were significant differences in the moments after injury with the pre-injury, for both assessment instruments. With Von Frey filament was observed a significant difference in group GN10 and GS in the final moments of evaluation. In comparisons between groups were not statistically significant differences obtained with any assessment instrument. CONCLUSION: Treatment with physical exercise was not effective in reducing pain in rats subjected to experimental sciatica model.


Assuntos
Animais , Ratos , Analgesia/métodos , Modelos Animais de Doenças , Terapia por Exercício , Compressão Nervosa , Neuropatia Ciática/reabilitação , Medição da Dor , Ratos Wistar
17.
Conf Proc IEEE Eng Med Biol Soc ; 2010: 1523-6, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-21096372

RESUMO

The channel bridging, signal regenerating, and functional rebuilding of injured nerves is one of the most important issues in life science research. In recent years, some progresses in the research area have been made in repairing injured nerves with microelectronic neural bridge. Based on the previous work, this paper presents a neural signal detection and functional electrical stimulation (FES) driving system with using high performance operational amplifiers, which has been realized. The experimental results show that the designed system meets requirements. In animal experiments, sciatic nerve signal detection, regeneration and function rebuilding between two toads have been accomplished successfully by using the designed system.


Assuntos
Amplificadores Eletrônicos , Materiais Biomiméticos , Terapia por Estimulação Elétrica/instrumentação , Eletrodiagnóstico/instrumentação , Neuropatia Ciática/diagnóstico , Neuropatia Ciática/reabilitação , Terapia Assistida por Computador/instrumentação , Animais , Bufo bufo , Desenho de Equipamento , Análise de Falha de Equipamento , Processamento de Sinais Assistido por Computador/instrumentação , Integração de Sistemas
18.
Neurorehabil Neural Repair ; 24(8): 736-45, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20702391

RESUMO

BACKGROUND: Electrical stimulation (ES) can enhance the regenerative capacity of axotomized motor and sensory neurons. However, the impact of ES on axonal regeneration and functional recovery has not been investigated in an animal model of a lengthy peripheral nerve defect. OBJECTIVE: To determine whether ES accelerates axonal regeneration and functional recovery of a 15-mm sciatic nerve defect in rats. METHODS: A 15-mm excision of the sciatic nerve was bridged with a chitosan scaffold with longitudinally or randomly oriented pores or with autologous grafting of the segment. In half of the animals with chitosan grafts, the proximal nerve stump was electrically stimulated for 1 hour at 20 Hz immediately after the nerve repair with the scaffolds. Axonal regeneration was investigated by retrograde labeling and morphometric analysis. The rate of motor functional recovery was evaluated by electrical nerve stimulation, behavioral tests of stepping, and histological appearance of the target muscles. RESULTS: Axonal regeneration and motor functional recovery were improved by ES in animals that received longitudinal pore grafts as compared with others. The maximal number of axons that regenerated across the longitudinal graft was achieved 2 to 4 weeks earlier in rats with ES. In addition, the latency of compound muscle action potentials (CMAPs), the peak amplitude of CMAPs, and nerve conduction velocity were improved by ES. Stepping indices were better, with less atrophy of target muscle in ES rats managed with longitudinal pores. CONCLUSION: Brief ES may accelerate axonal regeneration and motor recovery after focal peripheral nerve transection when repaired with optimally tissue-engineered grafts.


Assuntos
Estimulação Elétrica , Regeneração Tecidual Guiada/métodos , Atividade Motora , Regeneração Nervosa , Recuperação de Função Fisiológica , Nervo Isquiático/fisiopatologia , Neuropatia Ciática/reabilitação , Neuropatia Ciática/cirurgia , Tecidos Suporte , Potenciais de Ação , Animais , Axônios/patologia , Materiais Biocompatíveis/química , Quitosana/química , Modelos Animais de Doenças , Estimulação Elétrica/métodos , Eletrofisiologia , Regeneração Tecidual Guiada/instrumentação , Masculino , Ratos , Ratos Sprague-Dawley , Nervo Isquiático/lesões , Nervo Isquiático/patologia , Nervo Isquiático/cirurgia , Neuropatia Ciática/patologia , Neuropatia Ciática/fisiopatologia , Fatores de Tempo , Resultado do Tratamento , Caminhada
19.
Neurorehabil Neural Repair ; 24(8): 730-5, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20705804

RESUMO

BACKGROUND: The timing of electrical stimulation (ES) after peripheral nerve transection may enhance axonal regeneration and functional recovery. OBJECTIVE: The authors examined whether percutaneous ES at 1 mA and 2 Hz affects regeneration between the proximal and distal nerve stumps. METHODS: Four groups of adult rats were subjected to sciatic nerve section followed by repair using silicone rubber conduits across a 10-mm gap. All groups received ES for 15 minutes every other day for 2 weeks. Stimulation was initiated on day 1 following the nerve repair for group A, day 8 for group B, and day 15 for group C. The control group D received no ES. RESULTS: At 6 weeks after surgery in groups B and C, histological evaluations showed a significantly higher number of regenerated myelinated fibers in the sciatic nerve, and the electrophysiological results showed higher levels of reinnervation with relatively larger mean values of amplitudes, durations, and areas of compound muscle action potentials compared with A and D. CONCLUSION: A short delay in the onset of ES may improve the recovery of a severe peripheral nerve injury, which should be considered as a way of augmenting rehabilitative approaches.


Assuntos
Estimulação Elétrica/métodos , Músculo Esquelético/fisiopatologia , Regeneração Nervosa , Recuperação de Função Fisiológica , Nervo Isquiático/fisiopatologia , Neuropatia Ciática/reabilitação , Potenciais de Ação , Animais , Modelos Animais de Doenças , Eletrofisiologia , Músculo Esquelético/inervação , Bainha de Mielina/patologia , Ratos , Ratos Sprague-Dawley , Nervo Isquiático/lesões , Nervo Isquiático/patologia , Neuropatia Ciática/patologia , Neuropatia Ciática/fisiopatologia , Fatores de Tempo , Resultado do Tratamento
20.
Funct Neurol ; 25(1): 33-43, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20626995

RESUMO

The present study examined whether transplantation of mesenchymal stem cells (MSCs) in combination with exercise would have synergistic effects leading to functional recovery that is greater than exercise alone. Sprague-Dawley rats received a sciatic nerve transection and were divided into four groups: denervated (control), denervated + exercise (control+Ex), denervated + MSC transplantation (MSC), and denervated + MSC transplantation + exercise (MSC+Ex). A volume of 1 x 105 of MSCs was injected into the lesion site in the MSC-treated groups, and culture medium in the control animals. Twelve hours after surgery, a swimming exercise regime was begun: 30 minutes/day for seven days in the MSC+Ex and control+Ex groups. Functional assessments including sciatic function index (SFI), vertical locomotor activity (VA), ankle activity (AA), and electrophysiological studies were performed to monitor the functional recovery. Histological analysis was performed to assess nerve continuity and myelination. No significant differences in SFI, VA, AA and electrophysiological studies were found between the MSC+Ex and control+Ex groups. Also, a morphological study revealed prominent axonal degeneration in the injured nerves of all animals. The results revealed that any synergistic effect of MSC transplantation on functional recovery of swimming exercise-treated transected nerve that may have existed was negligible.


Assuntos
Transplante de Células-Tronco Mesenquimais/métodos , Condicionamento Físico Animal/métodos , Recuperação de Função Fisiológica/fisiologia , Neuropatia Ciática/reabilitação , Neuropatia Ciática/cirurgia , Animais , Comportamento Animal , Modelos Animais de Doenças , Masculino , Atividade Motora , Condução Nervosa/fisiologia , Ratos , Ratos Sprague-Dawley , Natação/fisiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA