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1.
Medicine (Baltimore) ; 98(45): e17913, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31702669

RESUMO

Nocardia is an opportunistic pathogen from environment, which is generally thought to infect immunosuppressed patients (ISPs), but recent studies showed it could also cause infections in immunocompetent patients (ICPs).The aim of this study was to compare the clinical characteristics, patients' outcome, Nocardia species' identification, and antibiotic susceptibility profiles of nocardiosis between ICPs and ISPs.The detailed clinical data were collected from all the nonrepetitive nocardiosis patients during 2011 and 2018, from a tertiary general hospital in Beijing, China. Then each Nocardia isolate was identified to species level by DNA sequencing. The antibiotic susceptibility testing was performed by E test method, and interpreted following CLSI M24 document. The clinical and microbiological characteristics between ICPs and ISPs were compared statistically.A total of 23 nonrepetitive nocardiosis patients with detailed clinical data were enrolled in this study. Among them, 9 were ICPs and 14 were ISPs. All the skin and soft tissue infections occurred in ICPs (33.3% vs 0%, P < .05). Bronchiectasis occurred more frequently in ICPs (44.4% vs 21.4%), whereas chronic kidney diseases and coinfection with aspergillosis occurred more frequently in ISPs (35.7% vs 0%, 35.7% vs 0%, respectively), although they did not reach the statistical significance. There were no significant differences in other clinical characteristics, Nocardia species' identification, and antibiotic susceptibility between ISPs and ICPs (P > .05).Nocardiosis could occur in both ISPs and ICPs. Skin and soft tissue infection and bronchiectasis occurred more frequently in ICPs. Chronic kidney diseases and co-infection with aspergillosis occurred more frequently in ISPs. These characteristics should be noticed by physicians in diagnosis of nocardiosis.


Assuntos
Imunocompetência , Nocardiose/microbiologia , Nocardia/isolamento & purificação , Adulto , Idoso , Antibacterianos/uso terapêutico , China/epidemiologia , Feminino , Humanos , Masculino , Testes de Sensibilidade Microbiana/estatística & dados numéricos , Pessoa de Meia-Idade , Nocardia/efeitos dos fármacos , Nocardiose/classificação , Nocardiose/diagnóstico , Nocardiose/epidemiologia , Estudos Retrospectivos , Centros de Atenção Terciária/estatística & dados numéricos
3.
Int J Syst Evol Microbiol ; 69(10): 3116-3120, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31339486

RESUMO

A novel actinomycete strain, designated CFH S0054T, was isolated from a soil sample collected from Xiaokongshan in Tengchong, Yunnan Province, in south-west PR China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain CFH S0054T belonged to the genus Nocardia. Based on the results of 16S rRNA gene sequence analysis, strain CFH S0054T was most closely related to Nocardia concava IFM 0354T (98.3 %), Nocardia inohanensis NBRC 100128T (97.9 %), Nocardia otitidiscaviarum NBRC 14405T (97.9 %) and Nocardia heshunensis CFH S0067T (97.8 %). Strain CFH S0054T contained C16 : 0, C18 : 1 ω9c, C18 : 0 10-methyl (TBSA) and summed feature 3 as major cellular fatty acids and MK-8 (H4ω-cycl) as the major respiratory quinone. The polar lipid profile included phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and unknown phospholipids. The cell wall contained meso-diaminopimelic acid, and the whole-cell hydrolysates were arabinose, galactose, glucose, fructose, ribose and mannose. The genome of strain CFH S0054T was 7.9 Mbp with a G+C content of 68.4 mol%, the average nucleotide identity (ANI) values between strain CFH S0054T and N. concava IFM0354T were low (ANIm<87.7 % and ANIb=84.1 %). On the basis of phylogenetic, physiological and chemotaxonomic data, strain CFHS0054T is considered to represent a novel species of the genus Nocardia, for which the name Nocardiayunnanensis sp. nov. is proposed, The type strain is CFH S0054T (DSM 46763T=JCM 30082T).


Assuntos
Nocardia/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Nocardia/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
4.
BMJ Case Rep ; 12(7)2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31300597

RESUMO

Central venous catheter-associated bacteraemia caused by Nocardia species is very rare; the diagnosis of nocardiosis in patients with cancer is challenging because its clinical presentation is varied, sometimes mimicking metastases, and the high index of clinical suspicion is required for prompt institution of therapy. Herein, we report a case of nocardial sepsis with native aortic valve endocarditis in a patient with breast cancer in whom multidisciplinary team involvement and prompt initiation of therapy have led to successful outcome.


Assuntos
Valva Aórtica/microbiologia , Neoplasias da Mama/terapia , Cateteres Venosos Centrais/microbiologia , Endocardite Bacteriana/microbiologia , Nocardiose/diagnóstico , Nocardia/isolamento & purificação , Radiografia Torácica , Sepse/microbiologia , Amicacina/uso terapêutico , Antibacterianos/uso terapêutico , Anticoagulantes/uso terapêutico , Valva Aórtica/diagnóstico por imagem , Valva Aórtica/cirurgia , Clopidogrel/uso terapêutico , Tosse , Endocardite Bacteriana/tratamento farmacológico , Endocardite Bacteriana/etiologia , Fadiga , Feminino , Cefaleia , Implante de Prótese de Valva Cardíaca , Humanos , Meropeném/uso terapêutico , Pessoa de Meia-Idade , Nocardiose/patologia , Nocardiose/terapia , Inibidores da Agregação de Plaquetas/uso terapêutico , Sepse/tratamento farmacológico , Resultado do Tratamento , Varfarina/uso terapêutico
5.
Clin Lab ; 65(6)2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-31232022

RESUMO

BACKGROUND: Invasive pulmonary aspergillosis and nocardia overlap in clinical and radiological presentations, so differentiating between nocardia and invasive pulmonary aspergillosis is confusing. Though sputum culture could distinguish between nocardia and aspergillus fumigatus, but for the ultimate diagnosis, sputum culture provided limited help. Here we report a case of a patient with positive G test and aspergillus fumigatus sputum culture mimic invasive pulmonary aspergillosis ultimately diagnosed as nocardia through bronchoalveolar lavage culture combined metagenomic next-generation sequencing (NGS). METHODS: Bronchoalveolar lavage culture combined metagenomic NGS for infectious diseases were performed for diagnosis. RESULTS: Bronchoalveolar lavage culture combined metagenomic next-generation sequencing showed Nocardia Gelsenkirchen. CONCLUSIONS: Positive G test and sputum culture were not specific, while bronchoalveolar lavage culture and NGS gave more information for a differential diagnosis between nocardia and aspergillus fumigatus.


Assuntos
Aspergillus fumigatus/isolamento & purificação , Aspergilose Pulmonar Invasiva/diagnóstico , Nocardiose/diagnóstico , Nocardia/isolamento & purificação , Escarro/microbiologia , beta-Glucanas/sangue , Aspergillus fumigatus/genética , Aspergillus fumigatus/fisiologia , Líquido da Lavagem Broncoalveolar/microbiologia , Diagnóstico Diferencial , Feminino , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Aspergilose Pulmonar Invasiva/microbiologia , Teste do Limulus , Pulmão/microbiologia , Pessoa de Meia-Idade , Nocardia/genética , Nocardia/fisiologia , Nocardiose/microbiologia , Sensibilidade e Especificidade
7.
Mycopathologia ; 184(2): 327-334, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30887177

RESUMO

Mycetoma is a chronic granulomatous infectious disease that can affect the skin, subcutaneous tissue, fascia and bone. It can be caused by filamentous bacteria or fungi and usually involves the legs and feet. Mycetoma is endemic in tropical and subtropical regions and is easily misdiagnosed in clinical practice because of its nonspecific clinical features and lack of awareness of the disease. Although mycetoma is very rare in mainland China, an increasing number of cases have been reported in recent years. Here, we report a case of mycetoma in a patient who was misdiagnosed many years before receiving the correct treatment, leading to disease progression and motion limitation. The grains that represent microorganismal colonies were important clues for diagnosis. We also reviewed reported cases of mycetoma in mainland China. The majority of cases were reported from southern regions. Actinomycetoma was more commonly reported than was eumycetoma. The causative agents of actinomycetoma included Nocardia brasiliensis, N. asteroides, N. otitidiscaviarum, N. ninae and Gordonia terrae, and the causative fungi of eumycetoma were identified as Madurella mycetomatis, Fonsecaea pedrosoi and Acremonium falciforme. Notably, the diagnosis of mycetoma was delayed from months to decades in all of the patients, likely due to a lack of clinical experience. Our literature review suggests the importance of increased awareness of mycetoma in clinical practice, especially in non-endemic regions. Further investigative studies are needed to determine the real incidence of the disease in China.


Assuntos
Micetoma/diagnóstico , Micetoma/microbiologia , Micetoma/patologia , Nocardia/isolamento & purificação , Adulto , Idoso , China/epidemiologia , Feminino , Fungos/classificação , Fungos/isolamento & purificação , Histocitoquímica , Humanos , Incidência , Masculino , Microscopia , Pessoa de Meia-Idade , Micetoma/epidemiologia , Nocardia/classificação , Pele/patologia , Adulto Jovem
8.
J Coll Physicians Surg Pak ; 29(3): 278-280, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30823959

RESUMO

Nocardia species usually cause opportunistic infections, and the frequency of these infections is increasing owing to the growing population of immunocompromised hosts. However, Nocardia may sometimes causes an infectious disease in immunocompetent hosts. Herein, we report two cases of pulmonary nocardiasis in immunocompetent individuals, whose chest computed tomography (CT) findings mimicked bronchiectasis. Samples of bronchalveolar lavage (BAL) fluid obtained by bronchoscopy showed filamentous, branching, gram-positive rods, acid-fast filamentous branching rods, and a colony of suspected Nocardia was cultured. Based on 16sRNA and hsp65 gene sequence analysis, case 1 was identified as N. cyriacigeorgica, but case 2 was not matched. The patients responded well to treatment with the combination of sulfamethoxazole and linezolid.


Assuntos
Bronquiectasia/diagnóstico por imagem , Linezolida/uso terapêutico , Nocardiose/diagnóstico por imagem , Nocardiose/tratamento farmacológico , Sulfametoxazol/administração & dosagem , Tomografia Computadorizada por Raios X/métodos , Bronquiectasia/diagnóstico , Diagnóstico Diferencial , Quimioterapia Combinada , Feminino , Seguimentos , Humanos , Imunocompetência/imunologia , Masculino , Pessoa de Meia-Idade , Nocardia/efeitos dos fármacos , Nocardia/isolamento & purificação , Nocardiose/diagnóstico , Medição de Risco , Índice de Gravidade de Doença , Resultado do Tratamento
9.
J Infect Chemother ; 25(7): 552-555, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30852105

RESUMO

Here, we present a case of disseminated nocardiosis, involving pneumonia, percutaneous abscess, and bacteremia, in a 67-year-old Japanese woman. She had also been treated for rheumatoid arthritis with prednisolone, methotrexate, and tocilizumab (interleukin-6 receptor inhibitor). Based on the 16S rRNA sequence analysis and a blast search, we identified the isolate as Nocardia brasiliensis. We discontinued methotrexate and tocilizumab on admission, and administered intravenous antimicrobial combination therapy for 6 weeks, followed by oral trimethoprim-sulfamethoxazole for 12 months, in total. Nocardia bacteremia is rare, often difficult to diagnose, and substantially fatal. However, due to our prompt diagnosis within one day of the onset of symptoms, and administration of appropriate treatment based on antimicrobial susceptibilities, this patient succeeded in surviving the infection. Not only microbiologists but also clinicians should be aware of the characteristic bacterial form of Gram/Kinyoun staining for early recognition of nocardiosis.


Assuntos
Antibacterianos/uso terapêutico , Anticorpos Monoclonais Humanizados/efeitos adversos , Bacteriemia/diagnóstico , Nocardiose/diagnóstico , Nocardia/isolamento & purificação , Idoso , Artrite Reumatoide/tratamento farmacológico , Artrite Reumatoide/imunologia , Bacteriemia/tratamento farmacológico , Bacteriemia/microbiologia , DNA Bacteriano/isolamento & purificação , Quimioterapia Combinada/métodos , Feminino , Violeta Genciana , Humanos , Nocardia/genética , Nocardiose/tratamento farmacológico , Nocardiose/microbiologia , Fenazinas , RNA Ribossômico 16S/genética , Resultado do Tratamento
11.
PLoS One ; 14(2): e0211989, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30802260

RESUMO

BACKGROUND: Transplant recipients are at risk of pulmonary nocardiosis, a life-threatening opportunistic infection caused by Nocardia species. Given the limitations of conventional diagnostic techniques (i.e., microscopy and culture), a polymerase chain reaction (PCR)-based assay was developed to detect Nocardia spp. on clinical samples. While this test is increasingly being used by transplant physicians, its performance characteristics are not well documented. We evaluated the performance characteristics of this test on bronchoalveolar lavage (BAL) fluid samples from lung transplant recipients (LTRs). METHODS: We prospectively included all BAL samples from LTRs undergoing bronchoscopy at our institution between December 2016 and June 2017 (either surveillance or clinically-indicated bronchoscopies). Presence of microbial pathogens was assessed using techniques available locally (including microscopy and 10-day culture for Nocardia). BAL samples were also sent to the French Nocardiosis Observatory (Lyon, France) for the Nocardia PCR-based assay. Transplant physicians and patients were blinded to the Nocardia PCR results. RESULTS: We included 29 BAL samples from 21 patients (18 surveillance and 11 clinically-indicated bronchoscopies). Nocardiosis was not diagnosed in any of these patients by conventional techniques. However, Nocardia PCR was positive in five BAL samples from five of the patients (24%, 95% confidence interval: 11-45%); four were asymptomatic and undergoing surveillance bronchoscopy, and one was symptomatic and was later diagnosed with influenza virus infection. None of the five PCR-positive patients died or were diagnosed with nocardiosis during the median follow-up of 21 months after the index bronchoscopy (range: 20-23 months). CONCLUSIONS: In this prospective study, Nocardia PCR was positive on BAL fluid from one fourth of the LTRs. Nocardia PCR-based assays should be used with caution on respiratory samples from LTRs because of the possible detection of airway colonization using this technique. Larger studies are required to determine the usefulness of the Nocardia PCR-based assay in transplant recipients.


Assuntos
Transplante de Pulmão/efeitos adversos , Nocardiose/diagnóstico , Nocardia/isolamento & purificação , Infecções Oportunistas/microbiologia , Adulto , Idoso , Bélgica , Líquido da Lavagem Broncoalveolar/microbiologia , Feminino , Humanos , Pessoa de Meia-Idade , Nocardia/genética , Projetos Piloto , Reação em Cadeia da Polimerase , Estudos Prospectivos , RNA Ribossômico 16S/genética , RNA Viral/genética , Sensibilidade e Especificidade
12.
Int J Infect Dis ; 81: 97-99, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30731130

RESUMO

A case of granulomatous hepatitis due to Nocardia is reported here. The case patient was a 63-year-old immunocompetent man who presented with persistent fever, weight loss, and malaise. Radiology suggested an enlarged liver with dense diffuse to multiple tiny micronodular areas of parenchymal involvement, possibly granulomatous. Liver biopsy showed necrotizing granulomas and anti-tuberculosis therapy was initiated, but the patient showed no improvement. A repeat liver biopsy showed similar histopathology; however PCR for Mycobacterium tuberculosis was negative, while MGIT 960 culture grew filamentous Gram-positive bacilli, acid-fast by 1% H2SO4, identified biochemically as Nocardia spp. 16S rRNA sequencing confirmed Nocardia spp. A diagnosis of granulomatous hepatitis due to Nocardia spp. was made. Treatment based on drug sensitivity testing was initiated, resulting in a resolution of symptoms. The patient's history revealed that stray dogs adopted by his family had skin lesions, likely canine distemper (two newborn puppies had died recently). Nocardia is known to co-infect animals with distemper. This could have been the possible source of a zoonotic infection to the case patient. Nocardia spp. are seldom reported from sites other than the lungs, skin, or brain; the current case highlights the involvement of the liver. Due to the granulomatous tissue response, it could represent a differential diagnosis of tuberculosis in such cases.


Assuntos
Antibacterianos/administração & dosagem , Doenças do Cão/microbiologia , Fígado/patologia , Nocardiose/microbiologia , Nocardia/isolamento & purificação , Zoonoses/microbiologia , Idoso , Animais , Doenças do Cão/transmissão , Cães , Evolução Fatal , Febre/microbiologia , Humanos , Masculino , Nocardia/classificação , Nocardiose/tratamento farmacológico , Nocardiose/veterinária , Reação em Cadeia da Polimerase , Perda de Peso , Zoonoses/tratamento farmacológico
13.
J Infect Public Health ; 12(2): 167-170, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30704871

RESUMO

Nocardia asteroides complex and Nocardia brasiliensis are common etiological agents of disseminated nocardiosis among immunocompromised individuals. Here we reported an uncommon case of disseminated nocardiosis with the involvement of lung, brain, soft tissue & pancreas by a rarely isolated species Nocardia asiatica in a HIV infected individual. Diagnosis was initially misinterpreted as tuberculosis based on the clinical and radiological findings. The isolate was identified to the species level with a 16S rRNA gene analysis & in vitro susceptibility was done as resistance is not uncommon among them. Clinical cure & radiological regression of lesions was observed except for brain after treatment with meropenem, amikacin & cotrimoxazole.


Assuntos
Infecções por HIV/complicações , Hospedeiro Imunocomprometido , Nocardiose/diagnóstico , Nocardiose/patologia , Nocardia/isolamento & purificação , Adulto , Amicacina/administração & dosagem , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Humanos , Masculino , Meropeném/administração & dosagem , Testes de Sensibilidade Microbiana , Nocardia/classificação , Nocardia/genética , Nocardiose/tratamento farmacológico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Resultado do Tratamento , Combinação Trimetoprima e Sulfametoxazol/administração & dosagem
14.
Intern Med ; 58(11): 1605-1611, 2019 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-30713323

RESUMO

We report a case of Nocardia exalbida (N.exalbida)-induced pneumonia in a 70-year old Japanese man with lung cancer and radiation pneumonitis. He initially received doripenem (1.5 g/day) for pneumonia treatment, and N.exalbida was identified by a clone library analysis of bronchoalveolar lavage fluid obtained from the pneumonia lesion. The doripenem dosage was therefore increased to 3.0 g/day with adjunctive trimethoprim/sulfamethoxazole, and his pneumonia improved. N. exalbida is susceptible to antibiotics; thus, in nocardiosis, N. exalbida infection might be associated with a good response to treatment, although its clinical findings are non-specific and similar to those of other Nocardia infections.


Assuntos
Neoplasias Pulmonares/complicações , Nocardiose/complicações , Nocardia/isolamento & purificação , Infecções Oportunistas/complicações , Pneumonite por Radiação/complicações , Idoso , Antibacterianos/uso terapêutico , Doripenem/uso terapêutico , Quimioterapia Combinada , Humanos , Masculino , Nocardia/classificação , Nocardiose/tratamento farmacológico , Nocardiose/microbiologia , Infecções Oportunistas/tratamento farmacológico , Combinação Trimetoprima e Sulfametoxazol/uso terapêutico
15.
Ophthalmic Surg Lasers Imaging Retina ; 50(1): 53-55, 2019 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-30640397

RESUMO

A woman with a past medical history of radiation and chemotherapy for multiple malignancies presented with bilateral, multifocal, elevated chorioretinal lesions associated with vitritis. The differential diagnosis included endogenous endophthalmitis and metastatic disease. Her extensive medical history and ill appearance at presentation in concert with vitritis and subretinal lesions was concerning for endogenous endophthalmitis. Although her vitreous sample was negative, her blood cultures were positive for Nocardia kropenstedtii and Nocardia farcinica. She was started on broad-spectrum antibiotic coverage but had brain lesions on presentation and ultimately passed away. This is the first reported case of Nocardia endophthalmitis in the setting of systemic N. kroppenstedtii infection. [Ophthalmic Surg Lasers Imaging Retina. 2019;50:53-55.].


Assuntos
Endoftalmite/diagnóstico , Infecções Oculares Bacterianas/diagnóstico , Nocardiose/diagnóstico , Nocardia/isolamento & purificação , Antibacterianos/uso terapêutico , Diagnóstico Diferencial , Endoftalmite/tratamento farmacológico , Endoftalmite/microbiologia , Infecções Oculares Bacterianas/tratamento farmacológico , Infecções Oculares Bacterianas/microbiologia , Feminino , Humanos , Imagem por Ressonância Magnética , Pessoa de Meia-Idade , Nocardiose/tratamento farmacológico , Nocardiose/microbiologia , Tomografia de Coerência Óptica
16.
Diagn Microbiol Infect Dis ; 94(2): 165-172, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30679058

RESUMO

Nocardia spp. is a pathogen responsible for a variety of clinical infections, ranging from skin and soft tissue infections, to the respiratory tract and central nervous system infections. Its epidemiological characteristics, including species distribution, clinical features, and antimicrobial susceptibility profiles, should be under surveillance for the prevention and treatment of nocardiosis. In the present study, over a 9-year period (from 2009 to 2017), 53 non-repetitive Nocardia isolates were collected from 8 tertiary general hospitals of 7 cities in China. These isolates were identified to species level by multilocus sequence analysis(MLSA). The clinical data were also reviewed. The susceptibilities to 10 commonly-used antibiotics for Nocardia were determined by E-test stripes, and the resistance rates, MIC50 and MIC90 to each antibiotic by different species were analyzed. Of 53 Nocardia isolates, N. farcinica was the most common species (24.5%, 13/53), followed by N. cyriacigeorgica (20.8%, 11/53), N. terpenica (15.1%, 8/53), N. abscessus (9.43%, 5/53), N. otitidiscaviarum (7.55%, 4/53), respectively. Furthermore, 31 Nocardia (58.5%) isolates were recovered from lower respiratory tract (sputum and BALF), 15 (28.3%) from superficial Infection, 3 (5.7%) from pleural effusion, 2 (3.8%) from CSF, and 1 from bone marrow and 1 from synovial fluid, respectively. The antibiotic resistance profiles varied between different Nocardia species. All Nocardia isolates were susceptible to linezolid, followed by imipenem and amikacin (both 92.5% susceptibility rate). N. terpenica, rarely documented elsewhere, showed a different antimicrobial susceptibility profile. In summary, herein, the clinical and antibiotic resistance features of Nocardia species reported would be helpful for understanding the diversity of Nocardia species circulating in China and for decision making in the context of empiric therapy.


Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana , Nocardiose/microbiologia , Nocardiose/patologia , Nocardia/classificação , Nocardia/efeitos dos fármacos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , China/epidemiologia , Cidades/epidemiologia , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Tipagem de Sequências Multilocus , Nocardia/genética , Nocardia/isolamento & purificação , Nocardiose/epidemiologia , Centros de Atenção Terciária , Adulto Jovem
17.
BMJ Case Rep ; 12(1)2019 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-30635309

RESUMO

The authors report a case of a lung abscess caused by Nocardia sp. in a previously healthy adolescent. A 17-year-old young man presented with tonsillitis that did not respond to ß-lactamic antibiotic, dyspnoea and thoracic pain. The X-ray revealed a cavitation in the right pulmonary upper lobe. He was admitted and completed a 14-day empirical antimicrobial therapy for a pulmonar abscess, although no clinical recovery was observed. He then underwent pulmonary biopsy of the lesion and PCR analysis of the collected pulmonary tissue, which revealed the presence of Nocardia This case emphasises the importance of considering nocardiosis in the differential diagnosis of a lung abscess, particularly if no response to empirical therapy is obtained.


Assuntos
Abscesso Pulmonar/diagnóstico por imagem , Nocardiose/diagnóstico por imagem , Nocardia/genética , Administração Intravenosa , Adolescente , Assistência ao Convalescente , Anti-Infecciosos/administração & dosagem , Anti-Infecciosos/uso terapêutico , Diagnóstico Diferencial , Humanos , Imunocompetência , Abscesso Pulmonar/tratamento farmacológico , Abscesso Pulmonar/microbiologia , Masculino , Nocardia/isolamento & purificação , Nocardiose/tratamento farmacológico , Nocardiose/microbiologia , Tomografia Computadorizada por Raios X , Tonsilite/tratamento farmacológico , Resultado do Tratamento
18.
J Basic Microbiol ; 59(3): 288-301, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30604885

RESUMO

Antagonistic haloalkaliphilic Nocardiopsis sp. AJ1 (GenBank JX575136.1), isolated and identified from the saline soil of Kovalam solar salterns was able to produce antimicrobial secondary metabolites and effectively suppressed several bacterial and fungal pathogens. The metabolite extracted from ethyl acetate precipitation suppressed the bacterial and fungal pathogens to the range between 2.14 and 20.14 mm and also controlled the shrimp killer virus WSSV by 83% than the control and significantly (p < 0.05) differed. GC-MS analysis revealed that, the ethyl acetate precipitation contains pyrrolo (1,2-A(pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-) and actinomycin C2. Non ribosomal peptide synthetase (NRPS) was amplified by PCR with the amplicon size of 750-800 bp length and further predicted the secondary structure by Iterative Threading Assembly Refinement (I-TASSER) bioinformatics approach. I-TASSER prediction helped to find out the secondary, 3-D structure, and ligand binding sites. The top ten modelling concluded that, the NRPS gene is closely similar to surfactin synthesizing gene, surfactin A synthetase C (SRFA-C). The findings revealed that, the active compounds from the secondary metabolites effectively suppressed the pathogenic bacteria, fungi, and virus and useful to develop antimicrobials.


Assuntos
Anti-Infecciosos/farmacologia , Bactérias/efeitos dos fármacos , Fungos/efeitos dos fármacos , Nocardia/química , Nocardia/isolamento & purificação , Vírus/efeitos dos fármacos , Acetatos/química , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Dactinomicina/análogos & derivados , Dactinomicina/química , Testes de Sensibilidade Microbiana , Modelos Moleculares , Nocardia/classificação , Nocardia/genética , Peptídeo Sintases/química , Peptídeo Sintases/genética , Filogenia , Pirazinas/química , Pirróis/química , RNA Ribossômico 16S/genética , Salinidade , Microbiologia do Solo
19.
Int J Syst Evol Microbiol ; 69(1): 159-164, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30489236

RESUMO

A novel actinobacterium, designated strain SYSU K10002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K10002T was most closely related to the type strains of Nocardiaaltamirensis NBRC 108246T (99.0 % sequence similarity) and Nocardiatenerifensis NBRC 101015T (98.8 %) and is therefore considered to represent a member of the genus Nocardia. DNA-DNA hybridization values between strain SYSU K10002T and the closely related type strains of the genus Nocardia were less than 70 %. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were arabinose, ribose and galactose. The major isoprenoid quinone was MK-8(H4,ω-cycl), while the major fatty acids (>10 %) were C16 : 0, C18 : 1ω9c and C18 : 0 10-methyl. The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unidentified glycolipid. Mycolic acids were present. The genomic DNA G+C content of strain SYSU K10002T was 67.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10002T represents a novel species of the genus Nocardia, for which the name Nocardiaaurea sp. nov. is proposed. The type strain is SYSU K10002T (=KCTC 39849T=DSM 103986T).


Assuntos
Cavernas/microbiologia , Nocardia/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Parede Celular/química , China , DNA Bacteriano/genética , Ácido Diaminopimélico/química , Ácidos Graxos/química , Ácidos Micólicos/química , Nocardia/isolamento & purificação , Hibridização de Ácido Nucleico , Peptidoglicano/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
20.
Ocul Immunol Inflamm ; 27(5): 762-765, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-29746787

RESUMO

Purpose: To describe a case of Nocardia subretinal abscess, which posed a diagnostic challenge due to the presence of mycobacterial genome in sample obtained from fine needle aspiration biopsy (FNAB). Methods: A retrospective chart review. Results: A 25-year-old male presented with sudden, painless onset diminution of vision of left eye and found to have placoid patch of choroiditis just temporal to the macula in right eye and showed vitritis, subretinal abscess with exudative retinal detachment and vitreous hemorrhage in left eye. Both aqueous and vitreous samples were negative microbiologically and polymerase chain reaction for various genomes. Sample obtained from FNAB was positive for mycobacterial genome and yielded Nocardia arthritidis on culture. She was treated with antitubercular therapy and intravenous and intravitreal antimicrobials. Conclusions: Nocardia subretinal abscess can be a diagnostic challenge in tuberculosis-endemic region, especially in conditions when there is co-infection with Mycobacterium tuberculosis.


Assuntos
Abscesso/microbiologia , Infecções Oculares Bacterianas/microbiologia , Nocardia/isolamento & purificação , Doenças Retinianas/microbiologia , Adulto , Antibacterianos/uso terapêutico , Antituberculosos/uso terapêutico , Humanos , Masculino
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