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1.
Int J Mol Sci ; 23(3)2022 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-35163073

RESUMO

Soil salinity is an important environmental factor affecting physiological processes in plants. It is possible to limit the negative effects of salt through the exogenous application of microelements. Silicon (Si) is widely recognized as an element improving plant resistance to abiotic and biotic stresses. The aim of the research was to determine the impact of foliar application of Si on the photosynthetic apparatus, gas exchange and DNA methylation of barley (Hordeum vulgare L.) grown under salt stress. Plants grown under controlled pot experiment were exposed to sodium chloride (NaCl) in the soil at a concentration of 200 mM, and two foliar applications of Si were made at three concentrations (0.05%, 0.1% and 0.2%). Measurements were made of relative chlorophyll content in leaves (CCl), gas exchange parameters (Ci, E, gs, and PN), and selected chlorophyll fluorescence parameters (Fv/Fm, Fv/F0, PI and RC/ABS). Additionally, DNA methylation level based on cytosine methylation within the 3'CCGG 5' sequence was analyzed. Salinity had a negative effect on the values of the parameters examined. Exogenous application of Si by spraying leaves increased the values of the measured parameters in plants. Plants treated with NaCl in combination with the moderate (0.1%) and highest (0.2%) dose of Si indicated the lowest methylation level. Decrease of methylation implicated with activation of gene expression resulted in better physiological parameters observed in this group of barley plants.


Assuntos
Epigênese Genética , Regulação da Expressão Gênica de Plantas , Hordeum/fisiologia , Fotossíntese , Folhas de Planta/fisiologia , Proteínas de Plantas/genética , Estresse Salino , Silício/farmacologia , Antioxidantes/farmacologia , Clorofila/metabolismo , Metilação de DNA , Hordeum/efeitos dos fármacos , Hordeum/genética , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/genética , Proteínas de Plantas/metabolismo , Salinidade , Oligoelementos/farmacologia
2.
Poult Sci ; 101(3): 101647, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-34998228

RESUMO

This study was designed to evaluate effects of source of macro and trace minerals (inorganic vs. organic) in fast and slower growing broiler breeders' diets on egg and hatchling mineral content and on offspring tibia morphological, biophysical, and mechanical characteristics. After 10 wk feeding the breeders (at 30 wk of age), eggs were collected and incubated. Eggs and hatchlings were analysed on mineral content. Male chickens were assigned to 32 pens with 12 chickens per pen. At approximately 1,700 and 2,600 gram BW, three chickens per pen were slaughtered. Tibia characteristics were determined. Organic minerals in the broiler breeder diet resulted in higher Fe and Se concentration in the egg and in higher Se concentration in the hatchling. Despite effects of mineral source on mineral concentration in the eggs and hatchlings were limited, organic minerals in the slower-growing broiler breeder diet resulted in higher offspring BW (d 42, Δ = 115 g; P = 0.03) and advanced tibia development (higher thickness (∆ = 0.38 cm; P < 0.001), osseous volume (∆ = 5.1 cm3; P = 0.01), and mineral density (Δ = 0.13 g/cm3; P = 0.03) at 2,600 g BW), but this was not observed in fast-growing chickens. This suggests that 1) the difference in feed intake of the breeders between strains might affect offspring performance, which might indicate that current slower-growing breeder diets might be suboptimal in minerals or that transgenerational mineral availability in slower growing chickens appears to be more effective on bone development, which might be related to time available for bone development. 2) transgenerational mineral availability in offspring appears to play a role via other mechanisms than via absolute mineral concentrations.


Assuntos
Oligoelementos , Ração Animal/análise , Animais , Galinhas , Dieta/veterinária , Masculino , Minerais , Óvulo , Tíbia , Oligoelementos/farmacologia
3.
Behav Brain Res ; 419: 113701, 2022 02 15.
Artigo em Inglês | MEDLINE | ID: mdl-34863808

RESUMO

Exposure to the metal vanadium, in both animals and humans has been linked to various physiological consequences including respiratory and gastrointestinal conditions. Research on the neurobehavioral effects of vanadium exposure is limited. Hence, the purpose of the current study was to examine the effects of chronic low-dose vanadium administration (0.04 mg/week) on the behavior of young male rats. Four weeks following the administration of vanadium, rats were tested on the open field, object recognition, and Morris Water maze tasks. Vanadium did not affect exploration, locomotion, or anxiety-like behavior as measured by the open field task. Vanadium administration affected novel object recognition performance. Intriguingly, rats exposed to vanadium exhibited lower latency times on day 2 of the Morris Water maze. These findings suggest that vanadium's behavioral effects are complex and warrant further investigation to better understand the potential benefits and consequences of its exposure.


Assuntos
Comportamento Animal/efeitos dos fármacos , Aprendizagem/efeitos dos fármacos , Locomoção/efeitos dos fármacos , Oligoelementos/farmacologia , Vanádio/farmacologia , Fatores Etários , Animais , Masculino , Ratos , Ratos Sprague-Dawley , Oligoelementos/administração & dosagem , Vanádio/administração & dosagem
4.
Int J Mol Sci ; 22(21)2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34769436

RESUMO

New promising manganese-containing nanobiocomposites (NCs) based on natural polysaccharides, arabinogalactan (AG), arabinogalactan sulfate (AGS), and κ-carrageenan (κ-CG) were studied to develop novel multi-purpose trophic low-dose organomineral fertilizers. The general toxicological effects of manganese (Mn) on the vegetation of potatoes (Solanum tuberosum L.) was evaluated in this study. The essential physicochemical properties of this trace element in plant tissues, such as its elemental analysis and its spectroscopic parameters in electron paramagnetic resonance (EPR), were determined. Potato plants grown in an NC-containing medium demonstrated better biometric parameters than in the control medium, and no Mn accumulated in plant tissues. In addition, the synthesized NCs demonstrated a pronounced antibacterial effect against the phytopathogenic bacterium Clavibacter sepedonicus (Cms) and were proved to be safe for natural soil microflora.


Assuntos
Antibacterianos/farmacologia , Clavibacter/efeitos dos fármacos , Manganês/toxicidade , Micronutrientes/farmacologia , Nanocompostos/química , Polissacarídeos/química , Solanum tuberosum/crescimento & desenvolvimento , Carragenina/química , Galactanos/química , Micronutrientes/química , Solanum tuberosum/efeitos dos fármacos , Solanum tuberosum/metabolismo , Solanum tuberosum/microbiologia , Oligoelementos/farmacologia
5.
Int J Mol Sci ; 22(20)2021 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-34681755

RESUMO

Endometriosis is a chronic, estrogen-dependent, inflammatory condition that is defined as the presence of endometrial glands and stroma outside the uterine cavity. Despite the progress in research into the mechanisms leading to the development of endometriosis, its cause has not yet been established. It seems to be possible that the formation of oxidative stress may be one of the main causes of the development of endometriosis. There is much research that studies the potential role of trace elements in the appearance of endometrial-like lesions. Most studies focus on assessing the content of selected trace elements in the blood, urine, or peritoneal fluid in women with endometriosis. Meanwhile, little is known about the content of these elements in endometrial-like implants, which may be helpful in developing the theory of endometriosis. Investigations that are more comprehensive are needed to confirm a hypothesis that some trace elements play a role in the pathomechanism of endometriosis.


Assuntos
Biomarcadores/sangue , Endometriose/etiologia , Oligoelementos/sangue , Animais , Líquido Ascítico/metabolismo , Endometriose/diagnóstico , Endometriose/epidemiologia , Feminino , Humanos , Oligoelementos/farmacologia , Oligoelementos/toxicidade
6.
Sci Rep ; 11(1): 20329, 2021 10 13.
Artigo em Inglês | MEDLINE | ID: mdl-34645888

RESUMO

Hormesis is a dose-response phenomenon observed in numerous living organisms, caused by low levels of a large number of stressors, among which metal ions. In cities, metal levels are usually below toxicity limits for most plant species, however, it is of primary importance to understand whether urban metal pollution can threaten plant survival, or, conversely, be beneficial by triggering hormesis. The effects of Cd, Cr and Pb urban concentrations were tested in hydroponics on three annual plants, Cardamine hirsuta L., Poa annua L. and Stellaria media (L.) Vill., commonly growing in cities. Results highlighted for the first time that average urban trace metal concentrations do not hinder plant growth but cause instead hormesis, leading to a considerable increase in plant performance (e.g., two to five-fold higher shoot biomass with Cd and Cr). The present findings, show that city habitats are more suitable for plants than previously assumed, and that what is generally considered to be detrimental to plants, such as trace metals, could instead be exactly the plus factor allowing urban plants to thrive.


Assuntos
Biomassa , Monitoramento Ambiental/métodos , Hormese/efeitos dos fármacos , Metais Pesados/análise , Plantas/efeitos dos fármacos , Poluentes do Solo/análise , Oligoelementos/farmacologia , Biodegradação Ambiental , Cádmio/química , Cromo/química , Cidades , Hidroponia , Chumbo , Metais , Fotossíntese , Desenvolvimento Vegetal/efeitos dos fármacos , Raízes de Plantas , Brotos de Planta , Solo
7.
Environ Sci Pollut Res Int ; 28(40): 55888-55904, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34490568

RESUMO

A large number of studies have been conducted for clarifying toxicological mechanisms of particulate matter (PM) aimed to investigate the physicochemical properties of PM and providing biological endpoints such as inflammation, perturbation of cell cycle, oxidative stress, or DNA damage. However, although several studies have presented some effects, there is still no consensus on the determinants of biological responses. This review attempts to summarize all past research conducted in recent years on the physicochemical properties of environmental PM in different places and the relationship between different PM components and PM potential cytotoxicity on the human lung epithelial cells. Among 447 papers with our initial principles, a total of 50 articles were selected from 1986 to April 2020 based on the chosen criteria for review. According to the results of selected studies, it is obvious that cytotoxicity in human lung epithelial cells is created both directly or indirectly by transition metals (such as Cu, Cr, Fe, Zn), polycyclic aromatic hydrocarbons (PAH), and ions that formed on the surface of particles. In the selected studies, the findings of the correlation analysis indicate that there is a significant relationship between cell viability reduction and secretion of inflammatory mediators. As a result, it seems that the observed biological responses are related to the composition and the physicochemical properties of the PMs. Therefore, the physicochemical properties of PM should be considered when explaining PM cytotoxicity, and long-term research data will lead to improved strategies to reduce air pollution.


Assuntos
Poluentes Atmosféricos , Hidrocarbonetos Policíclicos Aromáticos , Oligoelementos , Poluentes Atmosféricos/análise , Células Epiteliais , Humanos , Pulmão , Material Particulado/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , Oligoelementos/farmacologia
8.
Int J Mol Sci ; 22(16)2021 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-34445238

RESUMO

The trace element selenium (Se) is an essential part of the human diet; moreover, increased health risks have been observed with Se deficiency. A sufficiently high Se status is a prerequisite for adequate immune response, and preventable endemic diseases are known from areas with Se deficiency. Biomarkers of Se status decline strongly in pregnancy, severe illness, or COVID-19, reaching critically low concentrations. Notably, these conditions are associated with an increased risk for autoimmune disease (AID). Positive effects on the immune system are observed with Se supplementation in pregnancy, autoimmune thyroid disease, and recovery from severe illness. However, some studies reported null results; the database is small, and randomized trials are sparse. The current need for research on the link between AID and Se deficiency is particularly obvious for rheumatoid arthritis and type 1 diabetes mellitus. Despite these gaps in knowledge, it seems timely to realize that severe Se deficiency may trigger AID in susceptible subjects. Improved dietary choices or supplemental Se are efficient ways to avoid severe Se deficiency, thereby decreasing AID risk and improving disease course. A personalized approach is needed in clinics and during therapy, while population-wide measures should be considered for areas with habitual low Se intake. Finland has been adding Se to its food chain for more than 35 years-a wise and commendable decision, according to today's knowledge. It is unfortunate that the health risks of Se deficiency are often neglected, while possible side effects of Se supplementation are exaggerated, leading to disregard for this safe and promising preventive and adjuvant treatment options. This is especially true in the follow-up situations of pregnancy, severe illness, or COVID-19, where massive Se deficiencies have developed and are associated with AID risk, long-lasting health impairments, and slow recovery.


Assuntos
Doenças Autoimunes/tratamento farmacológico , COVID-19/tratamento farmacológico , Sistema Imunitário/efeitos dos fármacos , Selênio/farmacologia , Oligoelementos/farmacologia , Suplementos Nutricionais , Feminino , Humanos , Gravidez
9.
Int J Mol Sci ; 22(16)2021 Aug 17.
Artigo em Inglês | MEDLINE | ID: mdl-34445558

RESUMO

Cell-based therapy is a highly promising treatment paradigm in ischemic disease due to its ability to repair tissue when implanted into a damaged site. These therapeutic effects involve a strong paracrine component resulting from the high levels of bioactive molecules secreted in response to the local microenvironment. Therefore, the secreted therapeutic can be modulated by preconditioning the cells during in vitro culturing. Herein, we investigated the potential use of magnetic resonance imaging (MRI) probes, the "iron-quercetin complex" or IronQ, for preconditioning peripheral blood mononuclear cells (PBMCs) to expand proangiogenic cells and enhance their secreted therapeutic factors. PBMCs obtained from healthy donor blood were cultured in the presence of the iron-quercetin complex. Differentiated preconditioning PBMCs were characterized by immunostaining. An enzyme-linked immunosorbent assay was carried out to describe the secreted cytokines. In vitro migration and tubular formation using human umbilical vein endothelial cells (HUVECs) were completed to investigate the proangiogenic efficacy. IronQ significantly increased mononuclear progenitor cell proliferation and differentiation into spindle-shape-like cells, expressing both hematopoietic and stromal cell markers. The expansion increased the number of colony-forming units (CFU-Hill). The conditioned medium obtained from IronQ-treated PBMCs contained high levels of interleukin 8 (IL-8), IL-10, urokinase-type-plasminogen-activator (uPA), matrix metalloproteinases-9 (MMP-9), and tumor necrosis factor-alpha (TNF-α), as well as augmented migration and capillary network formation of HUVECs and fibroblast cells, in vitro. Our study demonstrated that the IronQ-preconditioning PBMC protocol could enhance the angiogenic and reparative potential of non-mobilized PBMCs. This protocol might be used as an adjunctive strategy to improve the efficacy of cell therapy when using PBMCs for ischemic diseases and chronic wounds. However, in vivo assessment is required for further validation.


Assuntos
Movimento Celular , Fibroblastos/fisiologia , Ferro/farmacologia , Leucócitos Mononucleares/fisiologia , Neovascularização Fisiológica , Quercetina/farmacologia , Cicatrização , Adulto , Antioxidantes/farmacologia , Meios de Cultivo Condicionados/farmacologia , Fibroblastos/citologia , Humanos , Leucócitos Mononucleares/citologia , Oligoelementos/farmacologia , Adulto Jovem
10.
Nutr Res ; 92: 109-128, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34284268

RESUMO

Novel coronavirus disease 2019 (COVID-19) has spread across the globe; and surprisingly, no potentially protective or therapeutic antiviral molecules are available to treat severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. However, zinc (Zn) and copper (Cu) have been shown to exert protective effects due to their antioxidant, anti-inflammatory, and antiviral properties. Therefore, it is hypothesized that supplementation with Zn and Cu alone or as an adjuvant may be beneficial with promising efficacy and a favorable safety profile to mitigate symptoms, as well as halt progression of the severe form of SARS-CoV-2 infection. The objective of this review is to discuss the proposed underlying molecular mechanisms and their implications for combating SARS-CoV-2 infection in response to Zn and Cu administration. Several clinical trials have also included the use of Zn as an adjuvant therapy with dietary regimens/antiviral drugs against COVID-19 infection. Overall, this review summarizes that nutritional intervention with Zn and Cu may offer an alternative treatment strategy by eliciting their virucidal effects through several fundamental molecular cascades, such as, modulation of immune responses, redox signaling, autophagy, and obstruction of viral entry and genome replication during SARS-CoV-2 infection.


Assuntos
Antivirais/farmacologia , COVID-19/tratamento farmacológico , Cobre/farmacologia , Oligoelementos/farmacologia , Zinco/farmacologia , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Humanos , SARS-CoV-2
11.
Theriogenology ; 172: 307-314, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34311222

RESUMO

Two studies were conducted to evaluate the effects of trace mineral supplementation on traditional and novel measures of bull fertility. In Experiment 1, 37 mature bulls received one of three dietary supplements daily for 71 d: 1) Supplement without Cu, Zn, and Mn (CON); 2) Supplement with Cu, Zn, and Mn sulfate (SULF); and 3) Supplement with basic Cu chloride, and Zn and Mn hydroxychloride (CHLR). In Experiment 2, 128 Angus or Angus-Hereford bull calves were maintained on a growing diet for 75 d (year 1) or 119 d (year 2) in Calan gate equipped pens without mineral supplementation. Bulls (n = 32 head/treatment) received one of four trace mineral supplements daily for 84 d: 1) Zn with no Cu (ZN), 2) Cu with no Zn (CU), 3) Cu and Zn (ZNCU), or 4) no Cu or Zn (CON). Bull fertility measures included a breeding soundness evaluation (BSE) and novel fertility measures conducted using flow cytometry. In mature bulls, final liver Zn concentration was positively correlated (P = 0.02) with sperm concentration (r = 0.31) and tended (P = 0.06) to be negatively correlated with acrosome damage (r = -0.39). Peripubertal bulls receiving ZNCU had greater ADG than bulls in the CU group (P = 0.05). Each BSE and novel fertility component improved from Day 0-84 in peripubertal bulls and were not affected (P > 0.10) by mineral supplementation. Bulls that received no supplement (CON) had greater (P < 0.01) percentage of sperm with distal midpiece reflex (6.9 vs 4.0% for CON and TM supplement, respectively) and Dag or Dag-like defect (2.6 vs 1.4% for CON and TM supplement, respectively) in their ejaculates. Sperm viability after 30 min of incubation were not affected by trace mineral supplementation, but after 3 h incubation, sperm viability tended to differ (P = 0.06) between treatments and tended to be less for CON bulls (48.5%) compared to ZNCU bulls (55.1%). Among contrast comparisons, trace mineral supplemented bulls had greater (P < 0.05) percentage of viable sperm at 3 h post collection and reactive oxygen resistant sperm than CON bulls. Addition of Zn to trace mineral containing Cu (ZNCU) improved (P < 0.05) percentage of sperm in the ejaculate with high mitochondrial energy potential and viable sperm with intact acrosome membrane. In summary, it appears the homeostasis mechanisms for bull trace mineral maintenance are extremely efficient and mineral supplementation of mature and peripubertal bulls did not have major improvements in any laboratory or chute-side measures of bull fertility, however bulls exposed to breeding or in environments with diet antagonists might respond differently.


Assuntos
Oligoelementos , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Bovinos , Suplementos Nutricionais , Fertilidade , Masculino , Oligoelementos/farmacologia , Zinco/farmacologia
12.
Sci Rep ; 11(1): 12329, 2021 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-34112842

RESUMO

Minerals play an important role in animal metabolism. Knowledge of mineral requirements allows well-formulated diets to be provided, which is the main factor that affects performance. To determine the macromineral and trace element requirements for growth and maintenance, thirty-eight 2-month-old Santa Ines lambs with initial body weight (BW) of 13.0 ± 1.49 kg were distributed in a factorial design with feeding levels (ad libitum, 30% and 60% feed restriction) and sex classes [castrated (CM) and intact males (IM)]. The net mineral requirements for gain were higher (P < 0.05) with increasing BW and average daily gain, except for Ca and Na, which remained constant as the empty BW (EBW) increased. The macromineral net requirement for maintenance (g/kg EBW0.75) and the true retention coefficient (k; %) were 0.0784 and 65.2 for Ca, 0.0926 and 80.0 for P, and 0.0379 and 59.0 for K, respectively. The k of Mg was higher (P < 0.05) for IM (11.3 for IM and 7.9 for CM). Sex did not affect (P > 0.05) the maintenance requirement of the trace elements Co, Cu, Zn and Cr which were 0.0015, 0.037, 0.698, and 0.0055 (mg/kg EBW0.75), respectively. Our study indicated that the Santa Ines net mineral requirements are different from the main nutritional requirements established by committees for sheep, which may result in unbalanced diets.


Assuntos
Ração Animal , Peso Corporal/efeitos dos fármacos , Minerais/metabolismo , Oligoelementos/metabolismo , Animais , Minerais/farmacologia , Ovinos/metabolismo , Ovinos/fisiologia , Oligoelementos/farmacologia
13.
Colloids Surf B Biointerfaces ; 204: 111822, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33984616

RESUMO

Biomineralization approaches have been increasingly adopted to synthesizing advanced materials with superior properties. Nevertheless, the potential influence of inorganic trace elements on the mineralization process of collagen has been rarely reported, despite of the significant progress achieved on exploiting the critical roles of organic polymers in regulating the collagen mineralization. To this aim, the potential roles of Si, Zn and Sr in regulating the mineralization of gelatin-hydroxyapatite (HA) composite fibers have been examined in this study. The results indicated that the incorporation of trace elements not only promoted the biomineralization of gelatin, but also led to drastic change in the mineralization behavior. In particular, the gelatin-SiHA sample showed uniform mineralization predominantly inside the fibers, with nucleation and growth directions along the c-axis of the gelatin fibers. On the contrary, the gelatin-HA sample showed nucleation outside the fibers and spherical mineral crystals on top of fibers, typical structure for heterogeneous nucleation. As the mineralization process proceeded, the gelatin-ZnHA and gelatin-SrHA samples evolved into having similar structure as the gelatin-SiHA sample, despite of showing totally different mineralization behaviors at early time. Overall, the incorporation of trace elements seemed to lower the nucleation barriers, led to a more homogeneous mineralization mode within the fiber region and formation of mineralized structures closer to those in natural bone. Moreover, mineralized samples with trace elements demonstrated improved adhesion and cytoskeleton organization of osteoblastic cells. Such finding would provide important insight for understanding the mineralization process and the optimal design of advanced biological materials.


Assuntos
Durapatita , Oligoelementos , Osso e Ossos , Gelatina , Oligoelementos/farmacologia , Zinco
14.
Clin Nutr ESPEN ; 43: 39-48, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-34024545

RESUMO

BACKGROUND: The enormous health impact of the COVID-19 pandemic has refocused attention on measures to optimize immune function and vaccine response. Dietary deficiencies of micronutrients can weaken adaptive immunity. The aim of this review was to examine links between micronutrients, immune function and COVID-19 infection, with a focus on nutritional risks in subgroups of the Swiss population. METHODS: Scoping review on the associations between selected micronutrients (vitamins D and C, iron, selenium, zinc, and n-3 PUFAs) and immunity, with particular reference to the Swiss population. These nutrients were chosen because previous EFSA reviews have concluded they play a key role in immunity. RESULTS: The review discusses the available knowledge on links between sufficient nutrient status, optimal immune function, and prevention of respiratory tract infections. Because of the rapid spread of the COVID-19 pandemic, controlled intervention studies of micronutrients in the context of COVID-19 infection are now underway, but evidence is not yet available to draw conclusions. The anti-inflammatory properties of n-3 PUFAs are well established. In Switzerland, several subgroups of the population are at clear risk of nutrient deficiencies; e.g., older adults, multiple comorbidities, obesity, pregnancy, and institutionalized. Low intakes of n-3 PUFA are present in a large proportion of the population. CONCLUSION: There are clear and strong relationships between micronutrient and n-3 PUFA status and immune function, and subgroups of the Swiss population are at risk for deficient intakes. Therefore, during the COVID-19 pandemic, as a complement to a healthy and balanced diet, it may be prudent to consider supplementation with a combination of moderate doses of Vitamins C and D, as well as of Se, Zn and n-3 PUFA, in risk groups.


Assuntos
COVID-19/tratamento farmacológico , Suplementos Nutricionais , Ácidos Graxos Ômega-3/uso terapêutico , Fatores Imunológicos/uso terapêutico , Oligoelementos/uso terapêutico , Vitaminas/uso terapêutico , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Ácido Ascórbico/farmacologia , Ácido Ascórbico/uso terapêutico , COVID-19/imunologia , Comorbidade , Ácidos Graxos Ômega-3/farmacologia , Feminino , Humanos , Fatores Imunológicos/farmacologia , Masculino , Micronutrientes/farmacologia , Micronutrientes/uso terapêutico , Estado Nutricional , Pandemias , Gravidez , SARS-CoV-2 , Selênio/farmacologia , Selênio/uso terapêutico , Suíça , Oligoelementos/farmacologia , Vitamina D/farmacologia , Vitamina D/uso terapêutico , Vitaminas/farmacologia , Zinco/farmacologia , Zinco/uso terapêutico
15.
J Trace Elem Med Biol ; 67: 126789, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34044222

RESUMO

COVID-19 is a kind of SARS-CoV-2 viral infectious pneumonia. This research aims to perform a bibliometric analysis of the published studies of vitamins and trace elements in the Scopus database with a special focus on COVID-19 disease. To achieve the goal of the study, network and density visualizations were used to introduce an overall picture of the published literature. Following the bibliometric analysis, we discuss the potential benefits of vitamins and trace elements on immune system function and COVID-19, supporting the discussion with evidence from published clinical studies. The previous studies show that D and A vitamins demonstrated a higher potential benefit, while Selenium, Copper, and Zinc were found to have favorable effects on immune modulation in viral respiratory infections among trace elements. The principles of nutrition from the findings of this research could be useful in preventing and treating COVID-19.


Assuntos
Ensaios Clínicos como Assunto , Oligoelementos/farmacologia , Vitaminas/farmacologia , Bibliometria , COVID-19/epidemiologia , Humanos , Sistema Imunitário/efeitos dos fármacos
16.
BMC Vet Res ; 17(1): 151, 2021 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-33836766

RESUMO

BACKGROUND: Selenium (Se) has been recognized as an essential micronutrient for nearly all forms of life. In recent decades, broiler responses to dietary Se supplemental levels and sources have received considerable attention. On environmental grounds, organic trace mineral utilization in practical broiler feeds has been defended due to its higher bioavailability. In such feeds, trace minerals are provided simultaneously in the same supplement as inorganic salts or organic chelates, a fact commonly ignored in assays conducted to validate organic trace mineral sources. The current assay aimed to investigate growth and biochemical responses, as well as Se retention of growing chicks fed diets supplemented with organic and inorganic Se levels and where the trace minerals (zinc, copper, manganese, and iron) were provided as organic chelates or inorganic salts according to Se source assessed. In so doing, a 2 × 5 factorial arrangement was used to investigate the effects of sodium selenite (SS) and selenium-yeast (SY) supplemented in feeds to provide the levels of 0, 0.08, 0.16, 0.24, and 0.32 mg Se/kg. RESULTS: Chicks fed selenium-yeast diets had body weight (BW), and average daily gain (ADG) maximized at 0.133 and 0.130 mg Se/kg, respectively. Both Se sources linearly increased (P < 0.05) the glutathione peroxidase (GSH-Px) activity in chick blood but higher values were observed in sodium selenite fed chicks (P < 0.05). Both Se sources influenced thyroid hormone serum concentrations (P < 0.05). Chicks fed SY exhibited greater retention of Se in the feathers (P < 0.05). Relative bioavailability of selenium yeast compared with SS for the Se content in carcass, feathers, total and Se retention were, 126, 116, 125 and 125%, respectively. SY supplementation resulted in lower liver Se concentration as Se supplementation increased (P < 0.05). CONCLUSIONS: Based on performance traits, the supplemental level of organic Se as SY in organic trace minerals supplement to support the maximal growth of broiler chicks is 0.133 mg Se/kg.


Assuntos
Ração Animal , Galinhas , Dieta/veterinária , Selênio/farmacologia , Oligoelementos/farmacologia , Fenômenos Fisiológicos da Nutrição Animal , Animais , Disponibilidade Biológica , Relação Dose-Resposta a Droga , Selênio/administração & dosagem , Selênio/farmacocinética , Oligoelementos/administração & dosagem
17.
Int J Mol Sci ; 22(9)2021 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-33919241

RESUMO

Glaucoma is a heterogeneous group of chronic neurodegenerative disorders characterized by a relatively selective, progressive damage to the retinal ganglion cells (RGCs) and their axons, which leads to axon loss and visual field alterations. To date, many studies have shown the role of various elements, mainly metals, in maintaining the balance of prooxidative and antioxidative processes, regulation of fluid and ion flow through cell membranes of the ocular tissues. Based on the earlier and current research results, their relationship with the development and progression of glaucoma seems obvious and is increasingly appreciated. In this review, we aimed to summarize the current evidence on the role of trace elements in the pathogenesis and prevention of glaucomatous diseases. Special attention is also paid to the genetic background associated with glaucoma-related abnormalities of physiological processes that regulate or involve the ions of elements considered as trace elements necessary for the functioning of the cells.


Assuntos
Glaucoma/metabolismo , Oligoelementos/metabolismo , Animais , Glaucoma/induzido quimicamente , Glaucoma/prevenção & controle , Humanos , Doenças Neurodegenerativas , Oligoelementos/farmacologia
18.
Drug Metab Dispos ; 49(6): 434-441, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33762296

RESUMO

Disulfiram, an antialcoholism drug, could potentially be repurposed as an anticancer drug because of the formation of copper(II) diethyldithiocarbamate (CuET) from dithiocarb (DTC, a reduced metabolite of disulfiram) and Cu2+ CuET exhibited preferential distribution to tumor tissues. This study investigated the mechanism of CuET accumulation in tumor tissues by employing MDA-MB-231 human breast cancer cells. The concentration of CuET in cells treated with DTC and Cu2+ in acidic culture medium (pH 6.8) was significantly higher than that of the control group (pH 7.4). Subsequently, the effects of pH on the uptake of DTC, Cu2+, and CuET were investigated separately. The acidic environment significantly increased the uptake rate of DTC and Cu2+ but had no effect on CuET. MDA-MB-231 cells overexpressing copper transporter hCTR1 were constructed to evaluate its intermediate role in CuET accumulation. After treatment with CuCl2 followed by DTC for 15 minutes, the levels of CuET and Cu2+ in hCTR1-overexpressed cells were 2.5 times as much as those of vector group. In the tumors of cancer xenograft models constructed by hCTR1-MDA-MB-231 cells, the concentrations of CuET and Cu were also significantly higher than those of control group. In conclusion, the acidic microenvironment of tumors can promote the enrichment of CuET in tumors through dual action. On the one hand, it can promote transmembrane transport of DTC by converting ionic DTC into molecular state. On the other hand, it enhances Cu2+ uptake by activating hCTR1, which ultimately leads to the enrichment of CuET. SIGNIFICANCE STATEMENT: Increasing evidence suggests that the antitumor activity of disulfiram is related to the formation of a copper(II) diethyldithiocarbamate (CuET) of its reducing metabolite dithiocarb with copper(II) ion, which is preferentially distributed in tumor tissues. We showed that the acidic microenvironment, a common feature of many solid tumor tissues, could promote intracellular CuET accumulation through dual action without changing CuET uptake. This result is helpful for the formulation of clinical dosage regimens of disulfiram in cancer treatment.


Assuntos
Dissulfiram/farmacologia , Neoplasias , Distribuição Tecidual , Microambiente Tumoral , Inibidores de Acetaldeído Desidrogenases/farmacologia , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Cobre/metabolismo , Transportador de Cobre 1/metabolismo , Reposicionamento de Medicamentos , Humanos , Concentração de Íons de Hidrogênio , Neoplasias/tratamento farmacológico , Neoplasias/metabolismo , Neoplasias/patologia , Distribuição Tecidual/efeitos dos fármacos , Distribuição Tecidual/fisiologia , Oligoelementos/farmacologia , Microambiente Tumoral/efeitos dos fármacos , Microambiente Tumoral/fisiologia
19.
J Cancer Res Ther ; 17(1): 266-268, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33723167

RESUMO

Selenium is obligatory for proper functioning of body as it is the part of enzyme protection system. Its both organic and inorganic forms are thought to be active as an antitumor agent. We trialed the different dosages (0 × 106 M, 2.7 × 106 M, 5.4 × 106 M, and 8.1 × 106 M) of sodium selenite given to the acute lymphocytic leukemia cell lines incubated for 24, 48, and 72 h. The ratios of dead cells to live cells when treated with sodium selenite were very high as compared to the control with no treatment. This dosage-dependent apoptosis increased with the incubation time.


Assuntos
Antineoplásicos/farmacologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamento farmacológico , Selenito de Sódio/farmacologia , Apoptose , Linhagem Celular Tumoral , Proliferação de Células , Humanos , Leucemia-Linfoma Linfoblástico de Células Precursoras/patologia , Oligoelementos/farmacologia
20.
Nutrients ; 13(2)2021 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-33572579

RESUMO

We examined, in a rat model of moderate environmental human exposure to cadmium (Cd), whether the enhanced intake of zinc (Zn) may protect against Cd-caused destroying the oxidative/antioxidative balance and its consequences in the brain. The intoxication with Cd (5 mg/L, 6 months) weakened the enzymatic (superoxide dismutase, glutathione peroxidase, catalase) and non-enzymatic (total thiol groups, reduced glutathione) antioxidative barrier decreasing the total antioxidative status and increased the concentrations of pro-oxidants (hydrogen peroxide, myeloperoxidase) in this organ and its total oxidative status. These resulted in the development of oxidative stress and oxidative modifications of lipids and proteins. The co-administration of Zn (30 and 60 mg/L enhancing this element intake by 79% and 151%, respectively) importantly protected against Cd accumulation in the brain tissue and this xenobiotic-induced development of oxidative stress and oxidative damage to lipids and proteins. Moreover, this bioelement also prevented Cd-mediated oxidative stress evaluated in the serum. The favorable effect of Zn was caused by its independent action and interaction with Cd. Concluding, the enhancement of Zn intake under oral exposure to Cd may prevent the oxidative/antioxidative imbalance and oxidative stress in the brain and thus protect against injury of cellular macromolecules in the nervous system.


Assuntos
Encéfalo/metabolismo , Cádmio/metabolismo , Exposição Ambiental/efeitos adversos , Estresse Oxidativo/efeitos dos fármacos , Oligoelementos/administração & dosagem , Zinco/administração & dosagem , Animais , Encéfalo/efeitos dos fármacos , Cádmio/administração & dosagem , Cádmio/toxicidade , Intoxicação por Cádmio/complicações , Intoxicação por Cádmio/metabolismo , Catalase/efeitos dos fármacos , Catalase/metabolismo , Água Potável , Glutationa Peroxidase/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Peróxido de Hidrogênio/metabolismo , Metabolismo dos Lipídeos/efeitos dos fármacos , Masculino , Modelos Animais , Oxirredução , Estresse Oxidativo/fisiologia , Peroxidase/metabolismo , Proteínas/metabolismo , Ratos , Ratos Wistar , Superóxido Dismutase/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Fatores de Tempo , Oligoelementos/metabolismo , Oligoelementos/farmacologia , Zinco/metabolismo , Zinco/farmacologia
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